Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems.human tissue plasminogen activator was the first recombinant therapy protein that successfully produced in chinese hamster ovary cells in 1986 and approved for clinical use. since then, more and more therapeutic proteins are being manufactured in mammalian cells, and the technologies for recombinant protein production in this expression system have developed rapidly, with the optimization of both upstream and downstream processes. one of the most promising strategies is expression vector cassett ...202032372203
one-step enrichment of intact glycopeptides from glycoengineered chinese hamster ovary cells.recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level. a rapid method to enrich intact glycopeptides is essential for the analysis of glycoproteins, especially for biopharmaceutical proteins. in this study, we established a one-step method for the rapid capture of intact glycopeptides for analysis by mass spectrometry. compared to the conventional sequential enrichment me ...202032363175
an efficient screening system in yeast to select a hyperactive piggybac transposase for mammalian applications.as non-viral transgenic vectors, the piggybac transposon system represents an attractive tool for gene delivery to achieve a long-term gene expression in immunotherapy applications due to its large cargo capacity, its lack of a trace of transposon and of genotoxic potential, and its highly engineered structure. however, further improvements in transpose activity are required for industrialization and clinical applications. herein, we established a one-plasmid effective screening system and a two ...202032357554
kcnq1 antibodies for immunotherapy of long qt syndrome type 2.patients with long qt syndrome (lqts) are predisposed to life-threatening arrhythmias. a delay in cardiac repolarization is characteristic of the disease. pharmacotherapy, implantable cardioverter-defibrillators, and left cardiac sympathetic denervation are part of the current treatment options, but no targeted therapy for lqts exists to date. previous studies indicate that induced autoimmunity against the voltage-gated kcnq1 k+ channels accelerates cardiac repolarization.202032354382
dynamic modeling of cho cell metabolism using the hybrid cybernetic approach with a novel elementary mode analysis strategy.chinese hamster ovary (cho) cell culture has a major importance on the production of biopharmaceuticals, including recombinant therapeutic proteins such as monoclonal antibodies (mab). mathematical modeling of biological systems can successfully assess metabolism complexity while providing logical and systematic methods for relevant genetic target and culture parameter identification toward cell growth and productivity improvements. most modeling approaches on cho cells have been performed under ...202032351947
subphysiological temperature induces pervasive alternative splicing in chinese hamster ovary cells.rna sequencing (rnaseq) has been widely used to associate alterations in chinese hamster ovary (cho) cell gene expression with bioprocess phenotypes; however, alternative messenger rna (mrna) splicing, has thus far, received little attention. in this study, we utilized rnaseq for transcriptomic analysis of a monoclonal antibody (mab) producing cho k1 cell line subjected to a temperature shift. more than 2,465 instances of differential splicing were observed 24 hr after the reduction of cell cult ...202032346860
a doubly auxotrophic cho-k1 cell line for the production of recombinant monoclonal antibodies.chinese hamster ovary (cho) cells are the most widely used mammalian hosts for recombinant protein production due to their hardiness, ease of transfection, and production of glycan structures similar to those in natural human monoclonal antibodies. to enhance the usefulness of cho-k1 cells we developed a new selection system based on double auxotrophy. we used crispr-cas9 to knockout the genes that encode the bifunctional enzymes catalyzing the last two steps in the de novo synthesis of pyrimidi ...202032346859
antimicrobial activity, toxicity and accumulated hard-tissue debris (ahtd) removal efficacy of several chelating agents.to evaluate the antimicrobial, toxicity and cleaning effectiveness of ethylenediaminetetraacetic acid (edta) and maleic acid (ma) alone and combined with cetrimide (ctr).202032344451
optimization of parameters affecting on cho cell culture producing recombinant erythropoietin.several factors may affect erythropoietin (epo) sugar structures including designing cell culture procedure, ph, concentration of additives, dissolved oxygen, and other physicochemical parameters. in this study, we investigated the influence of changes in effective parameters and compounds on the growth rate of chinese hamster ovary cell (cho) cells producing recombinant epo. cell culture was performed at different temperature, buffering conditions, and varied concentrations of additives such as ...202032336186
naturally occurring variants in the transmembrane and cytoplasmic domains of the human coxsackie- and adenovirus receptor have no impact on virus internalisation.the coxsackie- and adenovirus receptor (car) mediates homophilic cell-cell contacts and susceptibility to both human pathogenic viruses through its membrane-distal immunoglobulin domain. in the present study, we screened five missense variants of the human car gene for their influence on adenovector or coxsackievirus entry into chinese hamster ovary cells. the car variants facilitated virus internalisation to a similar extent as wild type car. this underlines car's presumed invariance and essent ...202032334832
short communication: the essential role of n-glycosylation in the transport activity of bovine peptide transporter 2.bovine peptide transporter 2 (bpept2), which mediates the absorption of di- and tripeptides in the bovine mammary gland, was predicted to contain multiple putative n-glycosylation sites of asparagine residues. n-linked glycosylation is proven to be essential for the folding, stability, localization, and substrate binding of nutrient transporters and could therefore potentially have an essential role in the function of bpept2. this study investigated the effect of mutagenesis of n-glycosylation s ...202032331895
enzyme capacity-based genome scale modelling of cho cells.chinese hamster ovary (cho) cells are most prevalently used for producing recombinant therapeutics in biomanufacturing. recently, more rational and systems approaches have been increasingly exploited to identify key metabolic bottlenecks and engineering targets for cell line engineering and process development based on the cho genome-scale metabolic model which mechanistically characterizes cell culture behaviours. however, it is still challenging to quantify plausible intracellular fluxes and d ...202032330653
biological filtering and substrate promiscuity prediction for annotating untargeted metabolomics.mass spectrometry coupled with chromatography separation techniques provides a powerful platform for untargeted metabolomics. determining the chemical identities of detected compounds however remains a major challenge. here, we present a novel computational workflow, termed extended metabolic model filtering (emmf), that aims to engineer a candidate set, a listing of putative chemical identities to be used during annotation, through an extended metabolic model (emm). an emm includes not only can ...202032326153
genotoxicity of micro- and nano-particles of kaolin in human primary dermal keratinocytes and fibroblasts.kaolin is a clay mineral with the chemical composition al2si2o5(oh)4. it is an important industrial material, and is also used as a white cosmetic pigment. we previously reported that fine particles of kaolin have genotoxic potency to chinese hamster ovary cho aa8 cells, and to the lungs of c57bl/6 j and icr mice. in the present study, we evaluated the genotoxicity of different particle sizes of kaolin using primary normal human diploid epidermal keratinocytes and primary normal human diploid de ...202032322315
dna nanovaccines prepared using lema antigen protect golden syrian hamsters against leptospira lethal infection.nanoparticles (nps) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the dna vaccination strategy against leptospirosis.202032321154
multiplex secretome engineering enhances recombinant protein production and purity.host cell proteins (hcps) are process-related impurities generated during biotherapeutic protein production. hcps can be problematic if they pose a significant metabolic demand, degrade product quality, or contaminate the final product. here, we present an effort to create a "clean" chinese hamster ovary (cho) cell by disrupting multiple genes to eliminate hcps. using a model of cho cell protein secretion, we predict that the elimination of unnecessary hcps could have a non-negligible impact on ...202032313013
a high-resolution measurement of nucleotide sugars by using ion-pair reverse chromatography and tandem columns.n-linked glycosylation is a cellular process transferring sugars from glycosyl donors to proteins or lipids. biopharmaceutical products widely produced by culturing mammalian cells such as chinese hamster ovary (cho) cells are typically glycosylated during biosynthesis. for some biologics, the n-linked glycan is a critical quality attribute of the drugs. nucleotide sugars are the glycan donors and impact the intracellular glycosylation process. in current analytical methods, robust separation of ...202032300845
development of a scale-up strategy for chinese hamster ovary cell culture processes using the kl a ratio as a direct indicator of gas stripping conditions.herein, we described a scale-up strategy focused on the dissolved carbon dioxide concentration (dco2 ) during fed-batch cultivation of chinese hamster ovary cells. a fed-batch culture process for a 2000-l scale stainless steel (ss) bioreactor was scaled-up from similarly shaped 200-l scale bioreactors based on power input per unit volume (p/v). however, during the 2000-l fed-batch culture, the dco2 was higher compared with the 200-l scale bioreactor. therefore, we developed an alternative approa ...202032298540
combining lipoic acid to methylene blue reduces the warburg effect in cho cells: from tca cycle activation to enhancing monoclonal antibody production.the warburg effect, a hallmark of cancer, has recently been identified as a metabolic limitation of chinese hamster ovary (cho) cells, the primary platform for the production of monoclonal antibodies (mab). metabolic engineering approaches, including genetic modifications and feeding strategies, have been attempted to impose the metabolic prevalence of respiration over aerobic glycolysis. their main objective lies in decreasing lactate production while improving energy efficiency. although yield ...202032298377
electroceutical silk-silver gel to eradicate bacterial infection.with more than 50% of bacteria resistant to standard antibiotics, new strategies to treat bacterial infection and colonization are needed. based on the concept of targeting the bacteria synergistically on various fronts, it is hypothesized that an electrical insult associated with antibacterial materials may be a highly effective means of killing bacteria. in this work, an injectable conductive gel based on silk fibroin (sf) and silver nanoparticles (ag-nps) is synthesized, capable of coating a ...202032293155
immunogenicity risk assessment for an engineered human cytokine analogue expressed in different cell substrates.the purpose of this article is to illustrate how performance of an immunogenicity risk assessment at the earliest stage of product development can be instructive for critical early decision-making such as choice of host system for expression of a recombinant therapeutic protein and determining the extent of analytical characterization and control of heterogeneity in co- and post-translational modifications. application of a risk-based approach for a hypothetical recombinant dna analogue of a hum ...202032291556
patches and blebs: a comparative study of the composition and biophysical properties of two plasma membrane preparations from cho cells.this study was aimed at preparing and characterizing plasma membranes (pm) from chinese hamster ovary (cho) cells. two methods of pm preparation were applied, one based on adhering cells to a poly-lysine-coated surface, followed by hypotonic lysis and removal of intracellular components, so that pm patches remain adhered to each other, and a second one consisting of bleb induction in cells, followed by separation of giant plasma membrane vesicles (gpmv). both methods gave rise to pm in sufficien ...202032290157
synthesis and biological evaluation of antibody drug conjugates based on an antibody expression system: conamax.antibody production for adcs (or in general) is commonly performed by cho-based platforms and limited by volumetric productivity, expensive downstream purification, and extended optimization timelines. the conamax platform is a novel microbial-based protein production and secretion system. a suite of synthetic biology tools have enabled high volumetric productivity (>1 g/l/d) and glycoengineering to produce simple and consistent human-like post-translational modifications. conamax can be enginee ...202032280859
safety evaluation of water extract of gastrodia elata blume: genotoxicity and 28-day oral toxicity studies.water extract of gastrodia elata blume (wge) has great potential as an anti-depressant and could be developed as a functional food. this study aims to assess the safety of wge using in vitro and in vivo genotoxicity assays and a 28-day oral toxicity study. results from a bacterial reverse mutation assay (ames test) using five salmonella typhimurium strains (ta98, ta100, ta102, ta1535, and ta1537) with or without metabolic activation (s9 system) showed that wge did not induce mutagenicity. nor di ...202032278877
changes of resurgent na+ currents in the nav1.4 channel resulting from an scn4a mutation contributing to sodium channel myotonia.myotonia congenita (mc) is a rare disorder characterized by stiffness and weakness of the limb and trunk muscles. mutations in the scn4a gene encoding the alpha-subunit of the voltage-gated sodium channel nav1.4 have been reported to be responsible for sodium channel myotonia (scm). the nav1.4 channel is expressed in skeletal muscles, and its related channelopathies affect skeletal muscle excitability, which can manifest as scm, paramyotonia and periodic paralysis. in this study, the missense mu ...202032276507
assessing additivity of cytotoxicity associated with disinfection byproducts in potable reuse and conventional drinking waters.recent studies used the sum of the measured concentrations of individual disinfection byproducts (dbps) weighted by their chinese hamster ovary (cho) cell cytotoxicity lc50 values to estimate the dbp-associated cytotoxicity of disinfected waters. this approach assumed that cytotoxicity was additive rather than synergistic or antagonistic. in this study, we evaluated whether this assumption was valid for mixtures containing dbps at the concentration ratios measured in authentic disinfected waters ...202032275830
the effect of amino acid supplementation in an industrial chinese hamster ovary process.the main goal in biosimilar development is to increase chinese hamster ovary (cho) viability and productivity while maintaining product quality. despite media and feed optimization during process development, depletion of amino acids still occurs. the aim of the work was to optimize an existing industrial fed batch process by preventing shortage of amino acids and to gather knowledge about cho metabolism. several process outputs were evaluated such as cell metabolism, cell viability, monoclonal ...202032274904
temperature down-shift modifies expression of upr-/erad-related genes and enhances production of a chimeric fusion protein in cho cells.low culture temperature enhances the cell-specific productivity of chinese hamster ovary (cho) cells expressing varied recombinant (r-) proteins, but the mechanisms remain unclear. regulation of unfolded protein response (upr) pathway genes, such as transcriptional regulatory factors and endoplasmic reticulum (er)-resident proteins, appear to be involved in the improvements of r-protein production under low temperature conditions. the transcriptional regulation of upr-specific targets is studied ...202032271992
a proteomic approach towards understanding crypoprotective action of me2so on the cho cell proteome.chinese hamster ovary (cho) cell lines are the most widely used in vitro cells for research and production of recombinant proteins such as rhgh, tpa, and erythropoietin. we aimed to investigate changes in protein profiles after cryopreservation using 2d-dige maldi-tof ms and network pathway analysis. the proteome changes that occur in cho cells between freshly prepared cells and cryopreserved cells with and without me2so were compared to determine the key proteins and pathways altered during rec ...202032259523
comparison of a novel homogeneous cyclic amp assay and a luciferase assay for measuring stimulating thyrotropin-receptor autoantibodies.stimulating thyrotropin-receptor antibodies (tsab) cause graves' disease (gd). we tested a novel homogeneous fluorescent 3',5' cyclic adenine monophosphate (camp) assay for the detection of tsab in a bioassay.202032257955
antiarrhythmic mechanisms of chinese herbal medicine dingji fumai decoction.dingji fumai decoction (dfd) is used to treat ventricular arrhythmia, and it has provided a very good curative effect. however, its cellular electrophysiological mechanism is unknown.202032256664
screening of new cell cycle suppressive compounds from marine-derived microorganisms in chinese hamster ovary cells.monoclonal antibodies (mabs) are active pharmaceutical ingredients in antibody drugs, produced mainly using recombinant chinese hamster ovary (cho) cells. the regulation of recombinant cho cell proliferation can improve the productivity of heterologous proteins. chemical compound approaches for cell cycle regulation have the advantages of simplicity and ease of use in industrial processes. however, cho cells have genetic and phenotypic diversity, and the effects of such compounds might depend on ...202032253091
development of magoh protein-overexpressing hek cells for optimized therapeutic protein production.in the pharmaceutical industry, the need for high levels of protein expression in mammalian cells has prompted the search for new strategies, including technologies to obtain cells with improved mechanisms that enhance its transcriptional activity, folding, or protein secretion. chinese hamster ovary (cho) cells are by far the most used host cell for therapeutic protein expression. however, these cells produce specific glycans that are not present in human cells and therefore potentially immunog ...202032249976
tailoring translational strength using kozak sequence variants improves bispecific antibody assembly and reduces product-related impurities in cho cells.optimal production of bispecific antibodies (bsab) requires efficient and tailored co-expression and assembly of two distinct heavy and two distinct light chains. here, we describe a novel technology to modulate the translational strength of antibody chains via kozak sequence variants to produce bsab in a single cell line. in this study, we designed and screened a large kozak sequence library to identify 10 independent variants that can modulate protein expression levels from approximately 0.2 t ...202032246763
clinical immunogenicity risk assessment for a fusion protein.this document highlights some relevant factors in the assessment of immunogenicity risk of fusion protein therapeutics. our aim is to highlight specific risks associated with this type of molecule, while also aligning with general risk assessment factors, through a hypothetical case study, where the therapeutic molecule of interest is a receptor-fc fusion protein (rff) expressed within a typical manufacturing process in chinese hamster ovary cells (cho). given that the components are comprised o ...202032246297
reduction of charge variants by cho cell culture process optimization.over the past decade, global interest in the development of therapeutic monoclonal antibodies (mabs) has risen rapidly. as therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (carla lucia et al. in plos one 6:e24071, 2011; donaghy in mabs 8:659-671, 2016; nasiri et al. in j cell physiol 9:6441-6457, 2018; teo et al. in cancer immunol immunother 61:2295-2309, 2012). recent advances in cell cul ...202032236800
a role of heparan sulphate proteoglycan in the cellular uptake of lipocalins ß-lactoglobulin and allergen fel d 4.lipocalins, small extracellular hydrophobic molecule carriers, can be internalized by a variety of different cells. however, to date receptors have only been identified for human lipocalins. here, we specifically investigated uptake mechanisms for lipocalins ß-lactoglobulin and fel d 4 in hela and chinese hamster ovary (cho) cells. we provide evidence that cell surface heparan sulphate proteoglycan is essential for internalization of these lipocalins. in hela cells, lipocalin uptake was inhibite ...202032229688
improved identification of host cell proteins in a protein biopharmaceutical by lc-ms/ms using the proteominer™ enrichment kit.host cell proteins (hcps) in biotherapeutics can be identified by the use of enzymatic digestion and lc-ms/ms analysis. however, the major challenge is that hcps are often present at very low levels in relation to the protein drug (low ppm-levels). in this study, the proteominer™ enrichment kit (bio-rad) was evaluated as a strategy to enable identification of hcps by lc-ms/ms by enrichment of low-abundant hcps and a simultaneous depletion of the high-abundant product protein. a recombinant prote ...202032229402
immunogenic and inflammatory responses to citrullinated proteins are enhanced following modification with malondialdehyde-acetaldehyde adducts.malondialdehyde-acetaldehyde adducts (maa) act as potent immune adjuvants and co-localize with citrullinated antigens in tissues effected by rheumatoid arthritis (ra). we sought to examine the role of maa-adducts in promoting ra-related autoimmunity and inflammation.202032224441
nutrient supplementation strategy improves cell concentration and longevity, monoclonal antibody production and lactate metabolism of chinese hamster ovary cells.a careful selection of culture mediums and feeds has become necessary to maximize yields of recombinant proteins during bioprocesses of mammalian cells. supplements contain a variety of concentrate nutrients, and their beneficial effects vary according to recombinant cell lines. in this study, the effects of powerfeed a on growth kinetics, productivity and cellular metabolism were evaluated for two chinese hamster ovary cell lines producing a monoclonal antibody in a batch culture. supplemented ...202032223359
a new class of quadruplex dna-binding nickel schiff base complexes.we have prepared six new nickel schiff base complexes via reactions of substituted benzophenones with different diamines in the presence of nickel(ii). these new complexes were then reacted with 1-(2-choroethyl)piperidine to afford a further six novel nickel(ii) schiff base complexes bearing pendant ethylpiperidine groups. the complexes bearing the ethylpiperidine moieties had greater solubility in water, and were therefore suitable for use in dna binding experiments. esi mass spectra of solutio ...202032219227
validation and identification of reference genes in chinese hamster ovary cells for fc-fusion protein production.in order to reveal potential genotype-phenotype relationship, rt-qpcr reactions are frequently applied which require validated and reliable reference genes. with the investigation on long-term passage and fed-batch cultivation of cho cells producing an fc-fusion protein, four new reference genes-akr1a1, gpx1, aprt, and rps16, were identified from 20 candidates with the aid of genorm, normfinder, bestkeeper, and δct programs and methods. this article provided more verified options in reference ge ...202032216463
pharmacological properties of whale shark (rhincodon typus) melanocortin-2 receptor and melancortin-5 receptor: interaction with mrap1 and mrap2.in the current study, the whale shark (ws; rhincodon typus) melanocortin-2 receptor (mc2r) co-expressed with wsmrap1 in chinese hamster ovary (cho) cells could be stimulated in a dose dependent manner by acth(1-24) with an ec50 of 2.6 × 10-10 m ± 9.7 × 10-11. when the receptor was expressed alone, stimulation was only observed at [10-6 m]. a comparable increase in sensitivity to stimulation by srdes-ac-αmsh was also observed when the receptor was co-expressed with wsmrap1. in addition, co-expres ...202032213301
a metabolic network-based approach for developing feeding strategies for cho cells to increase monoclonal antibody production.chinese hamster ovary (cho) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. to date, a variety of metabolic engineering approaches have been used to improve the productivity of cho cells. while genetic manipulations are potentially laborious in mammalian cells, rational design of cho cell culture medium or efficient fed-batch strategies are more popular approaches for bioprocess optimization. in this study, ...202032211960
spike protein recognition of mammalian ace2 predicts the host range and an optimized ace2 for sars-cov-2 infection.sars-cov-2 causes the recent global covid-19 public health emergency. ace2 is the receptor for both sars-cov-2 and sars-cov. to predict the potential host range of sars-cov-2, we analyzed the key residues of ace2 for recognizing s protein. we found that most of the selected mammals including pets (dog and cat), pangolin and circetidae mammals remained the most of key residues for association with s protein from sars-cov and sars-cov-2. the interaction interface between cat/dog/pangolin/chinese h ...202032201080
branched-chain amino acid catabolism promotes thrombosis risk by enhancing tropomodulin-3 propionylation in platelets.branched-chain amino acids (bcaas), essential nutrients including leucine, isoleucine, and valine, serve as a resource for energy production and the regulator of important nutrient and metabolic signals. recent studies have suggested that dysfunction of bcaa catabolism is associated with the risk of cardiovascular disease. platelets play an important role in cardiovascular disease, but the functions of bcaa catabolism in platelets remain unknown.202032200651
cytotoxicity of halogenated tyrosyl compounds, an emerging class of disinfection byproducts.halogenated amino acids and peptides are an emerging class of disinfection byproducts (dbps), having been detected in drinking water and in washed food products. however, the toxicological significance of these emerging dbps remains unclear. in this study, the cytotoxicity of eight halogenated tyrosyl compounds was investigated in chinese hamster ovary (cho) cells using real-time cell analysis (rtca). dihalogenated tyrosyl compounds are more cytotoxic than their monohalogenated analogues. the cy ...202032200635
cell-free protein synthesis: a promising option for future drug development.proteins are the main source of drug targets and some of them possess therapeutic potential themselves. among them, membrane proteins constitute approximately 50% of the major drug targets. in the drug discovery pipeline, rapid methods for producing different classes of proteins in a simple manner with high quality are important for structural and functional analysis. cell-free systems are emerging as an attractive alternative for the production of proteins due to their flexible nature without a ...202032198631
defects in galactose metabolism and glycoconjugate biosynthesis in a udp-glucose pyrophosphorylase-deficient cell line are reversed by adding galactose to the growth medium.udp-glucose (udp-glc) is synthesized by ugp2-encoded udp-glc pyrophosphorylase (ugp) and is required for glycoconjugate biosynthesis and galactose metabolism because it is a uridyl donor for galactose-1-p (gal1p) uridyltransferase. chinese hamster lung fibroblasts harboring a hypomrphic ugp(g116d) variant display reduced udp-glc levels and cannot grow if galactose is the sole carbon source. here, these cells were cultivated with glucose in either the absence or presence of galactose in order to ...202032188137
antibody-drug conjugates using mouse-canine chimeric anti-dog podoplanin antibody exerts antitumor activity in a mouse xenograft model.antibody-drug conjugates (adcs), which consist of a monoclonal antibody (mab), a linker, and a payload, can deliver a drug to cancer tissues. we previously produced an anti-dog podoplanin (dpdpn) mab, pmab-38, which reacts with dpdpn-expressing canine melanomas and squamous cell carcinomas (sccs), but not with dpdpn-expressing canine type i alveolar cells or lymphatic endothelial cells, indicating that pmab-38 possesses cancer specificity. in this study, we developed an adc, p38b-dm1, using the ...202032182186
cell function profiling to assess clone stability.in cell line development the identification of stable chinese hamster ovary cells for production is a critical but onerous task. the stability trial focus upon high-level attributes can mask profound underlying cellular changes, leading to unstable clones mistakenly being chosen for production. the challenge is to assay underlying cell pathways and subsystems without pushing up cell line development costs. chemstress® cell function profiling is a simple, multiwell plate-based assay that uses a p ...202032181887
hydrocyclones as cell retention device for cho perfusion processes in single-use bioreactors.in this study, a hydrocyclone (hc) especially designed for mammalian cell separation was applied for the separation of chinese hamster ovary cells. the effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in the recirculation loop were evaluated in batch separation tests. based on these preliminary batch tests, the hc was then integrated to 50-l disposable bioreacto ...202032181883
slowing of the time course of acidification decreases the acid-sensing ion channel 1a current amplitude and modulates action potential firing in neurons.acid-sensing ion channels (asics) are h+-activated neuronal na+ channels. they are involved in fear behavior, learning, neurodegeneration after ischemic stroke and in pain sensation. asic activation has so far been studied only with fast ph changes, although the ph changes associated with many roles of asics are slow. it is currently not known whether slow ph changes can open asics at all. here, we investigated to which extent slow ph changes can activate asic1a channels and induce action potent ...202032180707
multi-omics reveals impact of cysteine feed concentration and resulting redox imbalance on cellular energy metabolism and specific productivity in cho cell bioprocessing.chinese hamster ovary (cho) cells are currently the primary host cell lines used in biotherapeutic manufacturing of monoclonal antibodies (mabs) and other biopharmaceuticals. cellular energy metabolism and endoplasmic reticulum (er) stress are known to greatly impact cell growth, viability, and specific productivity of a biotherapeutic; but the molecular mechanisms are not fully understood. the authors previously employed multi-omics profiling to investigate the impact of a reduction in cysteine ...202032170810
evaluation of glycoprotein e subunit and live attenuated varicella-zoster virus vaccines formulated with a single-strand rna-based adjuvant.varicella-zoster virus (vzv), a human alphaherpesvirus 3, elicits both chickenpox and shingles and/or postherpetic neuralgia. a live attenuated vaccine (lav) and glycoprotein e (ge) subunit vaccine were developed to prevent vzv-induced diseases. we recently reported that single-strand rna (ssrna) based on the intergenic region of the internal ribosome entry site of cricket paralysis virus (crpv) is an effective adjuvant for protein-based and virus-like particle-based vaccines. here, chinese hams ...202032167678
review of the current methods of chinese hamster ovary (cho) cells cultivation for production of therapeutic protein.most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. the mammalian eukaryotic cells culture approaches, particularly the cho (chinese hamster ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. recent advances in cho cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. the developments of ...202032164511
effects of titanium dioxide nanoparticles on the hprt gene mutations in v79 hamster cells.the genotoxicity of anatase/rutile tio2 nanoparticles (tio2 nps, nm105 at 3, 15 and 75 µg/cm2) was assessed with the mammalian in-vitro hypoxanthine guanine phosphoribosyl transferase (hprt) gene mutation test in chinese hamster lung (v79) fibroblasts after 24 h exposure. two dispersion procedures giving different size distribution and dispersion stability were used to investigate whether the effects of tio2 nps depend on the state of agglomeration. tio2 nps were fully characterised in the previ ...202032150818
comparison of the pharmacological profiles of arginine vasopressin and oxytocin analogs at marmoset, macaque, and human vasopressin 1a receptor.arginine vasopressin (avp) and oxytocin (ot) are nonapeptides that bind to g-protein coupled receptors and influence social behaviors. consensus mammalian avp and ot (leu8-ot) sequences are highly conserved. in marmosets, an amino acid change in the 8th position of the peptide (pro8-ot) exhibits unique structural and functional properties. there is ∼85 % structural homology between the ot receptor (otr) and vasopressin 1a receptor (v1ar) resulting in significant cross-reactivity between the liga ...202032145592
influence of polymer charge on the localization and dark- and photo-induced toxicity of a potential type i photosensitizer in cancer cell models.a current trend within photo-dynamic therapy (pdt) is the development of molecular systems targeting hypoxic tumors. thus, type i pdt sensitizers could here overcome traditional type ii molecular systems that rely on the photo-initiated production of toxic singlet oxygen. here, we investigate the cell localization properties and toxicity of two polymeric anthracene-based fluorescent probes (neutral ant-phea and cationic ant-pim). the cell death and dna damage of chinese hamster ovary cancer cell ...202032138280
enhancement of sialylation in rigg in glyco-engineered chinese hamster ovary cells.since about 70% of commercial biopharmaceutical products have been produced in chinese hamster ovary (cho) cells, this cell line is undeniably a workhorse for biopharmaceuticals production. meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of igg antibodies. taking these findings together, we aimed to establish cho cell lines that highly produce sialic acid terminals by overexpressing two n-acetylneuraminic acid ...202032125558
ascorbic acid 2-glucoside pretreatment protects cells from ionizing radiation, uvc, and short wavelength of uvb.ascorbic acid 2-glucoside (aa2g), glucosylated ascorbic acid (aa), has superior properties for bioavailability and stability compared to aa. although aa2g has shown radioprotective properties similar to aa, effects for uv light, especially uvc and uvb, are not studied. aa2g was tested for cytotoxicity and protective effects against ionizing radiation, uvc, and broadband and narrowband uvb in chinese hamster ovary (cho) cells and compared to aa and dimethyl sulfoxide (dmso). pretreatment with dms ...202032106443
circulating ficolin-2 and ficolin-3 form heterocomplexes.the complement system constitutes an important part of the innate immune system. the collectins and the ficolins are soluble pattern recognition molecules that contribute to complement activation via the lectin pathway. during previous experiments with ficolin-2 and ficolin-3, we have observed that the molecules may interact. we therefore hypothesized the existence of stable ficolin-2/-3 heterocomplexes. we could demonstrate ficolin-2/-3 heterocomplexes in normal human serum and plasma by elisa ...202032094208
understanding the effect of high gas entrance velocity on chinese hamster ovary (cho) cell culture performance and its implications on bioreactor scale-up and sparger design.there are three main potential sources for cell shear damage existing in stirred tank bioreactors. one is the potential high energy dissipation in the immediate impeller zones; another from small gas bubble burst; and third is from high gas entrance velocity (gev) emitting from the sparger. while the first two have been thoroughly addressed for the scale-up of chinese hamster ovary (cho) cell culture knowing that a wide tolerable agitation range with non-damaging energy dissipation exists and th ...202032086806
optimized crispr/cas9 strategy for homology-directed multiple targeted integration of transgenes in cho cells.site-specific integration has emerged as a promising strategy for precise chinese hamster ovary (cho) cell line engineering and predictable cell line development (cld). crispr/cas9 with the homology-directed repair (hdr) pathway enables precise integration of transgenes into target genomic sites. however, inherent recalcitrance to hdr-mediated targeted integration (ti) of transgenes results in low targeting efficiency, thus requiring a selection process to find a targeted integrant in cho cells. ...202032086804
identification and functional characterization of a novel splicing variant in the f8 coagulation gene causing severe hemophilia a.we have identified a synonymous f8 variation in a severe hemophilia a (ha) patient who developed inhibitors following factor viii (fviii) prophylaxis. the unreported c.6273 g > a variant targets the consensus splicing site of exon 21.202032078252
altered gene expression in cho cells following polyamine starvation.to investigate the impact of polyamine deprivation on the transcriptome of cho cells results: polyamines play a central but poorly-understood role in cell proliferation. most studies to date have utilised chemical inhibitors to probe polyamine function. here we exploit the fact that cho cells grown in serum-free medium have an absolute requirement for putrescine supplementation due to their deficiency in activity of the enzyme arginase. a gene expression microarray (affymetrix) analysis of cho-k ...202032078082
improvement of intracellular traffic system by overexpression of kdel receptor 1 in antibody-producing cho cells.the localization of soluble endoplasmic reticulum (er) chaperones in the cell organelle is mediated by the c-terminal kdel (lysine, aspartic acid, glutamic acid and leucine) motif. this motif is recognized by the kdel receptor, a seven-transmembrane protein that cycles between the er and cis-golgi to capture missorted kdel chaperones from post-er compartments in a ph-dependent manner. the kdel receptor's target chaperones have a substantial role in protein folding and assembly. in this study, th ...202032073237
[eukaryotic expression, protein purification and biological effects research of human cs1-fc fusion protein].signaling lymphocyte activation family 7 (slamf7/cs1) is a cell surface glycoprotein that is highly expressed in multiple myeloma cells. cs1 is a sensitive and specific biomarker for multiple myeloma. car-t cell immunotherapy is a new method for the treatment of multiple myeloma. cs1 car-t cell immunotherapy has good effect on relapsed refractory multiple myeloma. to detect the expression efficiency of cs1 car on cs1 car-t cells and to find an auxiliary means to car-t cell immunotherapy, we prep ...202032072787
data for engineering lipid metabolism of chinese hamster ovary (cho) cells for enhanced recombinant protein production.the data presented in this article relates to the manuscript entitled 'engineering of chinese hamster ovary cell lipid metabolism results in an expanded er and enhanced recombinant biotherapeutic protein production', published in the journal metabolic engineering [1]. in the article here, we present data examining the overexpression of the lipid metabolism modifying genes scd1 and srebf1 in cho cells by densitometry of western blots and by using mass spectrometry to investigate the impact on spe ...202032071989
the use of catechins in chinese hamster ovary cell media for the improvement of monoclonal antibody yields and a reduction of acidic species.catechin compounds have potential benefits for recombinant monoclonal antibody (mab) production as chemical additives in cell culture media. in this study, four catechin compounds catechin (cat), epicatechin (ec), gallocatechin-gallate (gcg), and epigallocatechin-gallate (egcg) were added to cell culture media (at 50 μm) and their effects on the recombinant chinese hamster ovary (cho) cell culture, specific productivity, and mab quality were assessed. the results indicate that the improvement of ...202032067358
neutralizing antibody against osteopontin attenuates non-alcoholic steatohepatitis in mice.previously, we reported that an extracellular matrix protein, osteopontin (opn), is involved in various autoimmune diseases using a neutralizing polyclonal antibody against opn generated in rabbits. however, the antibody cannot be used for long-term mouse models of chronic inflammatory disease because of the induction of antibodies against anti-opn rabbit igg. in this study, we generated a new antibody, anti-mouse opn mouse igg (35b6). 35b6 inhibited the cell adhesion of mouse and human opn to c ...202032062834
cytotoxic and genotoxic effects of e-liquids and their potential associations with nicotine, menthol and phthalate esters.in this study, we determined dna damage and chromosome breakage (indicators of genotoxicity) and cell viability (an indicator of cytotoxicity) in human lymphoblastoid tk6 and chinese hamster ovary (cho) cells treated with 33 e-liquids using in vitro single cell gel (comet), micronucleus (mn), and trypan blue assays, respectively. we also measured the contents of nicotine, five phthalate esters, and dl-menthol in the e-liquids to examine their effects on dna damage, chromosome breakage, and cell ...202032058129
codon and amino acid content are associated with mrna stability in mammalian cells.messenger rna (mrna) degradation plays a critical role in regulating transcript levels in the cell and is a major control point for modulating gene expression. in yeast and other model organisms, codon identity is a powerful determinant of transcript stability, contributing broadly to impact half-lives. general principles governing mrna stability are poorly understood in mammalian systems. importantly, however, the degradation machinery is highly conserved, thus it seems logical that mammalian t ...202032053646
single-step synthesis and in vitro anti-mycobacterial activity of novel nitrofurantoin analogues.the emergence of drug-resistant tuberculosis (dr-tb) as well as the requirement for long, expensive and toxic drug regimens impede efforts to control and eliminate tb. therefore, there's a need for effective and affordable anti-mycobacterial agents which can shorten the duration of therapy and are active against mycobacterium tuberculosis (mtb) in both active and latent phases. nitrofurantoin (nft) is a hypoxic agent with activity against a myriad of anaerobic pathogens and, like the first-line ...202032044516
profiles of plant core-fucosylated n-glycans of acid alpha-glucosidases produced in transgenic rice cell suspension cultures treated with eight different conditions.recombinant human acid alpha-glucosidase (rhgaa) from chinese hamster ovary cells is the only approved treatment for patients with pompe disease. in this study, rhgaas were produced in transgenic rice cell suspension cultures under eight different conditions; untreated, 5 μm of 2-fluoro-l-fucose (2-ff), 50 μm of 2-ff, 100 μm of 2-ff, 100 μm of 2-ff + 0.5% pluronic f-68 (pf-68), 100 μm of 2-ff + 0.05% tween 20 (tw 20), 0.5% pf-68, and 0.05% tw 20. the n-glycans of eight rhgaas were analyzed using ...202032044029
c1q/tnf-related protein 6 is a pattern recognition molecule that recruits collectin-11 from the complement system to ligands.c1q/tnf-related protein (ctrp) 6 is a member of the ctrp protein family associated with the regulation of cellular and endocrine processes. ctrp6 contains collagen and globular structures, resembling the pattern recognition molecules (prms) of the classical and lectin complement pathways. we expressed human ctrp6 in chinese hamster ovary cells and investigated the binding to different putative ligands (acetylated bsa [acbsa], zymosan, mannan, and lps from escherichia coli and salmonella as well ...202032041782
engineering mammalian cells to produce plant-specific n-glycosylation on proteins.protein n-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells. yeast, plants and mammalian cells, however, produce n-glycans with distinct structural features. these species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines. here, we used reverse cros ...202032039452
the epilepsy of infancy with migrating focal seizures: identification of de novo mutations of the kcnt2 gene that exert inhibitory effects on the corresponding heteromeric kna1.1/kna1.2 potassium channel.the epilepsy of infancy with migrating focal seizures (eimfs; previously called malignant migrating partial seizures of infancy) are early-onset epileptic encephalopathies (eoee) that associate multifocal ictal discharges and profound psychomotor retardation. eimfs have a genetic origin and are mostly caused by de novo mutations in the kcnt1 gene, and much more rarely in the kcnt2 gene. kcnt1 and kcnt2 respectively encode the kna1.1 (slack) and kna1.2 (slick) subunits of the sodium-dependent vol ...202032038177
inhibition of cholesteryl ester synthesis by polyacetylenes from atractylodes rhizome.using activity guided purification, four known compounds, sesquiterpene atractylenolide iii (1), and the polyacetylenes 14-acetoxy-12-senecioyloxytetradeca-2e,8e,10e-trien-4,6-diyn-1-ol (2), 14-acetoxy-12-α-methylbutyl-2e,8e,10e-trien-4,6-diyn-1-ol (3), and 14-acetoxy-12-β -methylbutyl-2e,8e,10e-trien-4,6-diyn-1-ol (4), were isolated from a traditional herbal medicine, atractylodes rhizome. structurally similar 3 and 4 (3/4 mixture) were obtained as a mixture. in intact chinese hamster ovary (ch ...202032035699
protective effects of compounds from cimicifuga dahurica against amyloid beta production in vitro and scopolamine-induced memory impairment in vivo.cimicifuga dahurica has traditionally been used as an antipyretic, analgesic, and anti-inflammatory agent and as a treatment for uterine and anal prolapse. this study has investigated the potential beneficial effects of this medicinal plant and its components on alzheimer's disease (ad) with a focus on amyloid beta (aβ) production and scopolamine-induced memory impairment in mice. an ethanol extract from c. dahurica roots decreased aβ production in app-cho cells [chinese hamster ovarian (cho) ce ...202032031796
glucose monitoring in cell culture with online ultrasound-assisted near-infrared spectroscopy.near-infrared (nir) spectroscopic analysis for suspensions often requires a pretreatment by dilution or filtering of suspended solids to maintain the sensitivity of the measurements. an online ultrasound-assisted spectroscopy (uas) unit enabling pretreatment-free and noncontact analysis for bioprocessing is proposed and evaluated with a model suspension containing 3-μm-diameter polystyrene latex particles (psls) with the density of 5.1 × 108 counts/ml and chinese hamster ovary (cho) cell culture ...202032023047
differential effects of 2-hydroxypropyl-cyclodextrins on lipid accumulation in npc1-null cells.niemann-pick disease type c (npc) is an autosomal recessive disorder characterized by abnormal accumulation of free cholesterol and sphingolipids in lysosomes. the iminosugar miglustat, which inhibits hexosylceramide synthesis, is used for npc treatment, and 2-hydroxypropyl-β-cyclodextrin (hp-β-cd), a cyclic oligosaccharide derivative, is being developed to treat npc. moreover, therapeutic potential of 2-hydroxypropyl-γ-cyclodextrin (hp-γ-cd) was shown in npc models, although its mechanism of ac ...202032019132
3-quinuclidinyl-α-methoxydiphenylacetate: a multi-targeted ligand with antimuscarinic and antinicotinic effects designed for the treatment of anticholinesterase poisoning.racemic 3-quinuclidinyl-α-methoxydiphenylacetate (mb266) was synthesised. its activity at muscarinic acetylcholine receptors (machrs), and muscle and neuronal nicotinic acetylcholine receptors (nachrs), was compared to that of atropine and racemic 3-quinucidinyl benzilate (qnb) using a functional assay based on agonist-induced elevation of intracellular calcium ion concentration in cn21, chinese hamster ovary (cho) and shsy5y human cell lines. mb266 acted as an antagonist at acetylcholine recept ...202032017982
analysis of chemometric models applied to raman spectroscopy for monitoring key metabolites of cell culture.the food and drug administration (fda) initiative of process analytical technology (pat) encourages the monitoring of biopharmaceutical manufacturing processes by innovative solutions. raman spectroscopy and the chemometric modeling tool partial least squares (pls) have been applied to this aim for monitoring cell culture process variables. this study compares the chemometric modeling methods of support vector machine radial (svmr), random forests (rf), and cubist to the commonly used linear pls ...202032012476
feeding tricarboxylic acid cycle intermediates improves lactate consumption and antibody production in chinese hamster ovary cell cultures.media components play an important role in modulating cell metabolism and improving product titer in mammalian cell cultures. to sustain cell productivity, highly active oxidative metabolism is desired. here we explored the effect of tricarboxylic acid (tca) cycle intermediates supplementation on lactate metabolism and productivity in chinese hamster ovary fed-batch cultures. direct addition of 5 mm alpha-ketoglutarate (α-kg), malic acid, or succinic acid in the basal medium did not have any sig ...202032012447
structure-function relationships of the soluble form of the antiaging protein klotho have therapeutic implications for managing kidney disease.the fortuitously discovered antiaging membrane protein αklotho (klotho) is highly expressed in the kidney, and deletion of the klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (ckd). klotho functions as a co-receptor for fibroblast growth factor 23 (fgf23) signaling, whereas its shed extracellular domain, soluble klotho (sklotho), carrying glycosidase activity, is a humoral factor that regulates renal health. low sklotho in ckd is associated with diseas ...202032005658
anti-d monoclonal antibodies from 23 human and rodent cell lines display diverse igg fc-glycosylation profiles that determine their clinical efficacy.anti-d immunoglobulin (anti-d ig) prophylaxis prevents haemolytic disease of the fetus and newborn. monoclonal igg anti-ds (mab-ds) would enable unlimited supplies but have differed in efficacy in fcγriiia-mediated adcc assays and clinical trials. structural variations of the oligosaccharide chains of mab-ds are hypothesised to be responsible. quantitative data on 12 fc-glycosylation features of 23 mab-ds (12 clones, 5 produced from multiple cell lines) and one blood donor-derived anti-d ig were ...202032001734
lipid catabolism in starved yak is inhibited by intravenous infusion of β-hydroxybutyrate.lipid is the chief energy source for starved animals. β-hydroxybutyrate (bhba) is the main ketone body produced by lipid decomposition. in chinese hamster ovary (cho) cell experiment, it was found that bhba could be used not only as an energy substance, but also as a ligand of gpr109a for regulating lipid metabolism. however, whether bhba can regulate lipid metabolism of yaks, and its effective concentration and signal pathway are not clear. this study investigated the effects and mechanism of s ...202031952136
electron scattering in conventional cell flask experiments and dose distribution dependency.electron beam therapy (ebt) is commonly used for treating superficial and subdermal tumors. previous cellular radiosensitivity research using ebt may be underestimating the contribution from flask wall scattering and the corresponding dose distribution. single cell suspensions of chinese hamster ovary (cho) cells were plated on flasks and irradiated with 3, 4, 7, 9, and 18 mev energy electron beams from two different institutions, and the spatial locations of surviving colonies were recorded. ga ...202031949176
correction of the aprt gene using repair-polypurine reverse hoogsteen hairpins in mammalian cells.in this study, we describe the correction of single-point mutations in mammalian cells by repair-polypurine reverse hoogsteen hairpins (repair-pprhs). these molecules consist of (1) a pprh hairpin core that binds to a polypyrimidine target sequence in the double-stranded dna (dsdna), producing a triplex structure, and (2) an extension sequence homologous to the dna sequence to be repaired but containing the wild-type nucleotide instead of the mutation and acting as a donor dna to correct the mut ...202031945727
photoacoustic effect applied on model membranes and living cells: direct observation with multiphoton excitation microscopy and long-term viability analysis.the photoacoustic effect is generated when a variable light interacts with a strongly light-absorbing material. in water, it may produce hot bubbles and shock waves that could affect the integrity of nearby cellular membranes, opening transient pores (photoporation). in this study, we have evaluated the effect of pulsed laser-irradiated carbon nanoparticles (cnp) on model membranes and on chinese hamster ovary (cho) cells. fluorescence lifetime measurements of calcein-loaded liposomes support th ...202031941922
effective temperature shift strategy development and scale confirmation for simultaneous optimization of protein productivity and quality in chinese hamster ovary cells.temperature shifts to lower culture temperatures are frequently employed in the manufacturing of protein therapeutics in mammalian cells to improve productivity, viability, or quality attributes. the direction and extent to which a temperature shift affects productivity and quality may vary depending on the expression host and characteristics of the expressed protein. we demonstrated here that two chinese hamster ovary (cho) clones expressing different human monoclonal antibodies responded diffe ...202031930722
attenuating apoptosis in chinese hamster ovary cells for improved biopharmaceutical production.chinese hamster ovary (cho) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion usd in global sales. cho cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. genetic engineering strategies to reduce apoptosis in cho cells have been investigated with ...202031930480
kinetic modeling of chinese hamster ovary cell culture: factors and principles.as a host for therapeutic protein expression, chinese hamster ovary (cho) cells are widely utilized in the mainstream biopharmaceutical industry. cell culture process development plays an important role in transitioning laboratory research to manufacturing. among different mathematic tools, kinetic modeling is commonly achieved through analyzing cell culture data to design process parameters, optimize media, and scale up bioreactors. in this review, we examine key factors for upstream process de ...202031928250
targeted optical imaging of the glucagonlike peptide 1 receptor using exendin-4-irdye 800cw.the treatment of choice for insulinomas and focal lesions in congenital hyperinsulinism (chi) is surgery. however, intraoperative detection can be challenging. this challenge could be overcome with intraoperative fluorescence imaging, which provides real-time lesion detection with a high spatial resolution. here, a novel method for targeted near-infrared (nir) fluorescence imaging of glucagonlike peptide 1 receptor (glp-1r)-positive lesions, using the glp-1 agonist exendin-4 labeled with irdye 8 ...202031924726
different localization of lysosomal-associated membrane protein 1 (lamp1) in mammalian cultured cell lines.lysosomal-associated membrane protein 1 (lamp1) mainly localizes to lysosomes and late endosomes. we herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines. rat lamp1 fused to enhanced green fluorescent protein (egfp) mostly accumulated at a particular cytoplasmic area and barely co-localized with lysotracker® red dnd-99 in golden hamster kidney bhk-21 cells and chinese hamster ovary cho-k1 cells. golden hamster, chinese hamster, a ...202031907597
choice of host cell line is essential for the functional glycosylation of the fc region of human igg1 inhibitors of influenza b viruses.abs are glycoproteins that carry a conserved n-linked carbohydrate attached to the fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes. the host cell line used to produce igg plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final igg-fc glycosylation profile. in this study, we compare tw ...202031907284
direct optical detection of cell density and viability of mammalian cells by means of uv/vis spectroscopy.the critical process parameters cell density and viability during mammalian cell cultivation are assessed by uv/vis spectroscopy in combination with multivariate data analytical methods. this direct optical detection technique uses a commercial optical probe to acquire spectra in a label-free way without signal enhancement. for the cultivation, an inverse cultivation protocol is applied, which simulates the exponential growth phase by exponentially replacing cells and metabolites of a growing ch ...202031897554
genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion.in mammalian cells, >25% of synthesized proteins are exported through the secretory pathway. the pathway complexity, however, obfuscates its impact on the secretion of different proteins. unraveling its impact on diverse proteins is particularly important for biopharmaceutical production. here we delineate the core secretory pathway functions and integrate them with genome-scale metabolic reconstructions of human, mouse, and chinese hamster ovary cells. the resulting reconstructions enable the c ...202031896772
summation of disinfection by-product cho cell relative toxicity indices: sampling bias, uncertainty, and a path forward.the cyto- and genotoxic potencies of disinfection by-products (dbps) have been evaluated in published literature by measuring the response of exposed chinese hamster ovary cells. in recent publications, dbp concentrations divided by their individual toxicity indices are summed to predict the relative toxicity of a water sample. we hypothesized that the omission or inclusion of certain dbps over others is equivalent to statistical sampling bias and may result in biased conclusions. to test this h ...202031894210
Refresh
firstprev
12345678910...
nextlast
Displaying items 201 - 300 of 26566