Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| differential role of r-met-hu g-csf on male reproductive function and development in prepubertal domestic mammals. | the understanding of mammalian spermatogenesis niche factors active during sexual development may be leveraged to impact reproduction in farm animals. the aim of this study was to evaluate the effects of r-met-hu/g-csf (filgrastim) on prepubertal sexual development of guinea pigs (cavia porcellus) and ram lambs (ovis aries). individuals of both species were administered r-met-hu/g-csf daily for 4 days. during and after administration protocols, testicular function and development were assessed t ... | 2019 | 31557198 |
| early integration of pastoralism and millet cultivation in bronze age eurasia. | mobile pastoralists are thought to have facilitated the first trans-eurasian dispersals of domesticated plants during the early bronze age (ca 2500-2300 bc). problematically, the earliest seeds of wheat, barley and millet in inner asia were recovered from human mortuary contexts and do not inform on local cultivation or subsistence use, while contemporaneous evidence for the use and management of domesticated livestock in the region remains ambiguous. we analysed mitochondrial dna and multi-stab ... | 2019 | 31480978 |
| technical recommendations for artificial insemination in sheep. | artificial insemination (ai) was the first important biotechnology applied to improve the genetics of farm animals. it allows the rapid and massive diffusion of desirable characteristics of males with high productive potential. we describe the different types of estrus induction and synchronization techniques and the use of the ai with fresh, chilled or frozen semen. through the adequacy of the protocols of estrus synchronization and ai to the different production systems, the efficient use of r ... | 2019 | 32368257 |
| heart rate sensor validation and seasonal and diurnal variation of body temperature and heart rate in domestic sheep. | advantages of low input livestock production on large pastures, including animal welfare, biodiversity and low production costs are challenged by losses due to undetected disease, accidents and predation. precision livestock farming (plf) enables remote monitoring on individual level with potential for predictive warning. body temperature (tb) and heart rate (hr) could be used for early detection of diseases, stress or death. we tested physiological sensors in free-grazing norwegian white sheep ... | 2019 | 32734092 |
| superficial swellings in sheep (ovis aries) and goats (capra hircus): clinical and ultrasonographic findings. | this study describes the clinical presentation of superficial swellings and evaluates the utility of ultrasonography in the diagnosis of such swellings in sheep and goats. ninety-three sheep and 73 goats were included in this study on the basis of presence of superficial swellings on the head (n=56), neck (n=16), chest wall (n=3), abdominal wall (n=40), umbilicus (n=14), scrotum (n=16), testes (n=1), udder (n=7), limbs (n=2), gluteal region (n=5), tail (n=1), and penile urethra (n=5). ultrasonog ... | 2019 | 31391383 |
| antibacterial and antibiotic modifying activity evaluation of ruminants' body fat used as zootherapeutics in ethnoveterinary practices in northeast brazil. | northeast brazilian ethnoveterinary studies associated with the medicinal use of zootherapies have shown that ruminants' body fat such as sheep (ovis aries), goats (capra hircus) and cows (bos taurus) are used in diseases affecting domestic animals. | 2019 | 30592980 |
| echinococcus canadensis g8 tapeworm infection in a sheep, china, 2018. | we report a sheep infected with echinococcus canadensis g8 tapeworm in china in 2018. this pathogen was previously detected in moose, elk, muskox, and mule deer in europe and north america; our findings suggest a wider host range and geographic distribution. surveillance for the g8 tapeworm should be conducted in china. | 2019 | 31211942 |
| dna methylation and mrna expression of col6a3 in antler mesenchyme of female and male reindeer. | reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. alpha 3(vi) collagen gene (col6a3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. | 2019 | 31134592 |
| genetic structure of mycoplasma ovipneumoniae informs pathogen spillover dynamics between domestic and wild caprinae in the western united states. | spillover diseases have significant consequences for human and animal health, as well as wildlife conservation. we examined spillover and transmission of the pneumonia-associated bacterium mycoplasma ovipneumoniae in domestic sheep, domestic goats, bighorn sheep, and mountain goats across the western united states using 594 isolates, collected from 1984 to 2017. our results indicate high genetic diversity of m. ovipneumoniae strains within domestic sheep, whereas only one or a few strains tend t ... | 2019 | 31653889 |
| prevalence of anti-hepatitis e virus antibodies in domestic animal from three representative provinces of burkina faso. | four major genotypes of hepatitis e virus (hev) have been documented worldwide (1-4) with genotypes 1 and 2 found in human in sub-saharan africa. human hepatitis cases due to hev genotype 3 and 4 are zoonotic with various animal identified as possible reservoirs. recently, hev genotype 3 was found in pigs and human beings in west africa, which may change the epidemic in human. here, we assessed the prevalence of hev antibodies in various domestic and wild mammalians in burkina faso. random sampl ... | 2019 | 32734080 |
| mycoplasma ovis infection in domestic sheep (ovis aries) in the united states: prevalence, distribution, associated risk factors, and associated outcomes. | mycoplasma ovis is a hemotropic bacterium reported to infect sheep, goats, and deer species. infection in these species can result in anemia, jaundice, and ill-thrift. although of worldwide distribution, only rare reports of this bacterium in the united states exist. the objectives of this retrospective study were to identify the prevalence and distribution of m. ovis, and identify associated demographic and management risk factors, and reproductive and production outcomes associated with infect ... | 2019 | 31472359 |
| serological epidemiological investigation of tibetan sheep (ovis aries) plague in qinghai, china. | the plague, which is caused by the gram-negative coccobacillus bacterium yersinia pestis, has been classified as a reemerging infectious disease by the world health organization. the qinghai-tibet plateau natural plague focus is the largest plague focus in china, and marmota himalayana is the primary host of the plague. tibetan sheep (ovis aries) were first identified as naturally infected hosts of y. pestis based on etiological evidence in 1975, and activities such as slaughtering or skinning t ... | 2019 | 30256745 |
| evaluation of serologic testing of rams in the management of brucella ovis in a domestic sheep flock. | brucella ovis is a bacterial pathogen present in most major sheep-producing regions of the world. the pathogen is associated with ram infertility, decreased ewe conception rates, and premature lambs. twenty elisa seropositive or indeterminate rams were culled from a b. ovis-positive flock, and donated to the wyoming state veterinary laboratory for evaluation of infection. tissues from each ram were collected at autopsy for additional testing, including bacterial culture, pcr, and histopathology. ... | 2019 | 30541385 |
| molecular characterization of peste des petits ruminants viruses in the marmara region of turkey. | recent outbreaks of peste des petits ruminants (ppr) in the marmara region of turkey including the european part of thrace is important due to its proximity to europe (greece and bulgaria) and the potential threat of spread of ppr into mainland europe. in order to investigate the circulation of pprv in the region suspect clinical and necropsy samples were collected from domestic sheep (n = 211) in the marmara region of turkey between 2011 and 2012. ppr virus (pprv) genome was detected in 10.4% ( ... | 2019 | 30525310 |
| comprehensive transcriptional profiling of the gastrointestinal tract of ruminants from birth to adulthood reveals strong developmental stage specific gene expression. | one of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. using a subset of rna-seq data from our high-resolution atlas of gene expression in sheep (ovis aries) we have provided the first comprehensive characterization of transcription of the entire gastrointestinal (gi) tract during the transition from pre-ruminant to ruminant. the dataset comprises 164 tissue samples from sheep at four different time points (birth ... | 2019 | 30530642 |
| genetic and morphometric categorization of taenia ovis from sheep in iran. | little is known about the genetic and morphological characters of taenia ovis. the purpose of the present study was to characterize sheep isolates of t. ovis using rostellar hook morphometry as well as mitochondrial genes sequence analysis. ninety sheep specimens of cysticercus ovis were collected from 18 slaughterhouses in iran. the mean ± s.d. for total length of large and small hooks were 174.1 ± 6.4 and 116.7 ± 5.4 µm, respectively. co1 and 12s rrna sequence analysis showed 11 and nine haplo ... | 2019 | 30419971 |
| allelic nomenclature for the duplicated mhc class ii dq genes in sheep. | the principal mhc class ii molecules involved in the presentation of peptides to the antigen specific receptors on cd4+ t cells genes in sheep are derived from dr and dq genes. allelic nomenclature systems for the drb1 and its partner dra loci are available for ovid's; however, no official nomenclature is available for the dq genes which creates ambiguity within the research community. ovine mhc haplotypes include at least two pairs of dqa and dqb genes, termed dqa1, dqb1 and dqa2, dqb2 and both ... | 2019 | 30415411 |
| traditional halal slaughter without stunning versus slaughter with electrical stunning of sheep (ovis aries). | thirty nellore crossbred male sheep (ovis aries) were divided into two groups of 15 animals each and subjected to either pre-slaughter electrical stunning followed by slaughter (st) or traditional halal slaughter without stunning (nst) to investigate the changes in blood biochemical parameters, meat quality and proteomic profile. higher (p < .05) ph, water holding capacity and warner-bratzler shear force were observed in meat from stunned sheep. quantitative proteomic approach using dige was emp ... | 2019 | 30388477 |
| studies on clinical anatomy of the maxillofacial and mandibular regions of the madras red sheep (ovis aries) in india. | the present study aimed to study the clinical anatomy of the maxillofacial and mandibular regions of the madras red sheep through the morphometric analysis of some clinically important parameters pertaining to the regional anaesthesia. | 2019 | 30371934 |
| differences in the thermal sensitivity and seminal quality of distinct ovine genotypes raised in tropical conditions. | for different ovine breeds to maximize their reproductive capacity in countries with tropical climate, it is important to evaluate their potential for thermal resilience and consequences on their reproductive traits. therefore, the objective of this study was to evaluate the effect of thermal environment temperatures of climate seasons in a tropical climate region on the surface temperatures of the scrotum, testicular biometric characteristics, seminal quality and serum testosterone concentratio ... | 2019 | 30292856 |
| climatic influences on the mortality of sheep during long-distance sea transport. | research on the causes of sheep death in sea voyages from australia to the middle east is limited, in particular little is known about the influence of climatic factors. mortality data from 417 shipments of sheep exported over an 11-year period (november 2004 to june 2015) were modelled retrospectively to determine associated climatic factors. the statistical analysis were performed for both the full data set with 417 voyages based on actual and estimated departure and arrival dates and a restri ... | 2019 | 30280681 |
| first report of bovine viral diarrhea virus and mycobacterium avium subspecies paratuberculosis infection in tibetan sheep (ovis aries) in tibetan plateau, china. | bovine viral diarrhea virus (bvdv) and mycobacterium avium subspecies paratuberculosis (map) are important pathogens, which cause serious disease in animals. however, information about bvdv and map infection in tibetan sheep in china is limited. two thousand one hundred and eighty-seven blood samples were collected from tibetan sheep between april 2013 and march 2014 from the tibetan plateau and tested for bvdv and map antibodies using commercial elisa kits. the overall seroprevalence of bvdv an ... | 2019 | 30269235 |
| comparative mrna and mirna expression in european mouflon (ovis musimon) and sheep (ovis aries) provides novel insights into the genetic mechanisms for female reproductive success. | prolific breeds of domestic sheep (ovis aries) are important genetic resources due to their reproductive performance, which is characterized by multiple lambs per birth and out-of-season breeding. however, the lack of a comprehensive understanding of the genetic mechanisms underlying the important reproductive traits, particularly from the evolutionary genomics perspective, has impeded the efficient advancement of sheep breeding. here, for the first time, by performing rna-sequencing we built a ... | 2019 | 29784930 |
| serological survey for antibodies against pestiviruses in wyoming domestic sheep. | pestiviruses including bovine viral diarrhea virus type 1 (bvdv-1), bvdv-2 and border disease virus (bdv) have been reported in both sheep and cattle populations, together with the hobi-like, an emerging group of pestiviruses. pestivirus control programs in the united states have focused on the control of bvdv-1 and 2. the incidence of pestivirus infection in sheep in the united states and the risk of transmission between cattle and sheep populations are unknown. the aim of this study was to per ... | 2018 | 29778211 |
| genome-wide association study reveals novel genes for the ear size in sheep (ovis aries). | variations in ear size can be observed in livestock such as sheep; however, the genetic basis of variable ear size in sheep is still poorly understood. to investigate causative genes associated with ear size in sheep, a genome-wide association study was performed in 115 adult duolang sheep with different-sized floppy ears using the ovine infinium hd beadchip. we found 38 significant snps at the genome-wide or chromosome-wise 5% significance level after bonferroni correction. the most significant ... | 2018 | 29774586 |
| the tail in tropical hair ewes (ovis aries) that are in estrus is used as a proceptive signal and favors ram' copulation. | two experiments were performed to determine if the tail has a role: 1) in proceptive behavior, and 2) in facilitating copulation in hair sheep. the movements and position of the tail of estrous and non-estrous ewes in response to male courtship were compared in the first study. in the second study, the courtship of rams and mating behavior directed towards tailed or tail-docked, in estrus or diestrus ewes, were compared. both experiments were conducted with restrained ewes during 3 min assessmen ... | 2018 | 29764712 |
| conceptus loss in santa inês ewes carrying twin pregnancies by natural mating or embryo transfer. | commercial application of reproductive biotechnologies such as multiple ovulation and embryo transfer depends on its overall efficiency. sheep embryo transfer is gradually gaining wider adoption, but pregnancy rates after embryo transfer remain lower than those derived from natural mating for most breeds. the work was aimed to evaluate embryonic and fetal losses in santa inês ewes carrying twin pregnancies by natural mating or embryo transfer. ewes were subjected to synchronized natural mating b ... | 2018 | 29742481 |
| single nucleotide polymorphisms in the hira gene affect litter size in small tail han sheep. | maintenance of appropriate levels of fecundity is critical for efficient sheep production. opportunities to increase sheep litter size include identifying single gene mutations with major effects on ovulation rate and litter size. whole-genome sequencing (wgs) data of 89 chinese domestic sheep from nine different geographical locations and ten australian sheep were analyzed to detect new polymorphisms affecting litter size. comparative genomic analysis of sheep with contrasting litter size detec ... | 2018 | 29734691 |
| tissue residue depletion of moxidectin in lambs (ovis aries) following subcutaneous administration. | to date, a tissue depletion study of moxidectin (mox) in lambs is not available. thus, considering that lamb meat is of great commercial interest in the world, the aim of the present study was to determine the residue levels of mox in lamb target-tissues (muscle, liver, kidney and fat) and subsequently calculate the mox withdrawal period. for this purpose, the target-tissues were analysed by ultra-high-performance liquid chromatography-tandem mass spectrometry. method validation was performed ba ... | 2018 | 29718774 |
| genome-wide association analyses highlight the potential for different genetic mechanisms for litter size among sheep breeds. | reproduction is an important trait in sheep breeding as well as in other livestock. however, despite its importance the genetic mechanisms of litter size in domestic sheep (ovis aries) are still poorly understood. to explore genetic mechanisms underlying the variation in litter size, we conducted multiple independent genome-wide association studies in five sheep breeds of high prolificacy (wadi, hu, icelandic, finnsheep, and romanov) and one low prolificacy (texel) using the ovine infinium hd be ... | 2018 | 29692799 |
| the use of a mechanical non-penetrating captive bolt device for the euthanasia of neonate lambs. | a non-penetrating captive bolt device, powered by a 1-grain 0.22″ cartridge delivering a calculated kinetic energy of 47 joules was tested as a euthanasia method on 200 neonate lambs (ovis aries) of 4.464 kg (sd (standard deviation) ± 1.056) mean dead weight, to assess effectiveness and shot position. every lamb (n = 200) was effectively stunned when the weapon was applied powered by a brown, 1-grain cartridge but 10/200 (5%) of the lambs displayed rhythmic or agonal breathing and were subsequen ... | 2018 | 29614834 |
| an independent validation study of loci associated with nematode resistance in sheep. | gastrointestinal nematode infection is a constraint on sheep production worldwide. selective breeding programmes to enhance resistance to nematode infection are currently being implemented in a number of countries. identification of loci associated with resistance to infection or causative mutations for resistance would enable more effective selection. loci associated with indicator traits for nematode resistance has been identified in previous studies. in this study, scottish blackface, texel a ... | 2018 | 29570808 |
| genomic selection signatures in sheep from the western pyrenees. | the current large spectrum of sheep phenotypic diversity results from the combined product of sheep selection for different production traits such as wool, milk and meat, and its natural adaptation to new environments. in this study, we scanned the genome of 25 sasi ardi and 75 latxa sheep from the western pyrenees for three types of regions under selection: (1) regions underlying local adaptation of sasi ardi semi-feral sheep, (2) regions related to a long traditional dairy selection pressure i ... | 2018 | 29566643 |
| melatonin decreases pulmonary vascular remodeling and oxygen sensitivity in pulmonary hypertensive newborn lambs. | background: chronic hypoxia and oxidative stress during gestation lead to pulmonary hypertension of the neonate (phn), a condition characterized by abnormal pulmonary arterial reactivity and remodeling. melatonin has strong antioxidant properties and improves pulmonary vascular function. here, we aimed to study the effects of melatonin on the function and structure of pulmonary arteries from phn lambs. methods: twelve lambs (ovis aries) gestated and born at highlands (3,600 m) were instrumented ... | 2018 | 29559926 |
| seroprevalence and risk factors of neospora caninum infection among domestic sheep in henan province, central china. | this study aimed to determine the frequency of antibodies to neospora caninum in domestic sheep raised in henan province, central china. serum samples from 779 domestic sheep were collected from march 2015 to may 2016, and antibodies to n. caninum were evaluated using an enzyme-linked immunosorbent assay (elisa). the results showed an overall igg positive rate of 7.32% (57/779). the risk factors significantly related to seropositivity to n. caninum in sheep were the age, the presence of dogs, an ... | 2018 | 29557776 |
| quantification and decomposition of environment-selection relationships. | in nature, selection varies across time in most environments, but we lack an understanding of how specific ecological changes drive this variation. ecological factors can alter phenotypic selection coefficients through changes in trait distributions or individual mean fitness, even when the trait-absolute fitness relationship remains constant. we apply and extend a regression-based approach in a population of soay sheep (ovis aries) and suggest metrics of environment-selection relationships that ... | 2018 | 29518255 |
| sheep genome functional annotation reveals proximal regulatory elements contributed to the evolution of modern breeds. | domestication fundamentally reshaped animal morphology, physiology and behaviour, offering the opportunity to investigate the molecular processes driving evolutionary change. here we assess sheep domestication and artificial selection by comparing genome sequence from 43 modern breeds (ovis aries) and their asian mouflon ancestor (o. orientalis) to identify selection sweeps. next, we provide a comparative functional annotation of the sheep genome, validated using experimental chip-seq of sheep t ... | 2018 | 29491421 |
| identification and characterization of micrornas in the intestinal tissues of sheep (ovis aries). | sheep are small ruminants, and their long intestines exhibit high digestive and absorptive capacity in many different rearing conditions; however, the genetic bases of this characteristic remains unclear. micrornas (mirnas) play a major role in maintaining both intestinal morphological structure as well as in regulating the physiological functions of this organ. however, no study has reported on the mirna expression profile in the intestinal tissues of sheep. here, we analyzed and identified the ... | 2018 | 29489866 |
| in silico investigation of the prion protein glycosylation profiles in relation to scrapie disease resistance in domestic sheep (ovis aries). | the prion protein is a membrane-bound glycoprotein which consists mainly α-helix structure. in contrast, the infectious prion protein shows the beta-sheet structure. the prion-associated diseases are all lethal neurodegenerative abnormalities, called transmissible spongiform encephalopathies. scrapie is the most common type of these illnesses affecting sheep, goats, and moufflon. the vrq, ahq, arr and n146s polymorphisms in the sheep prion gene have been found to be associated with resistance to ... | 2018 | 30261281 |
| microsatellite-based genetic structure and diversity of local arabian sheep breeds. | the genetic diversity of the sheep breeds in the arab countries might be considered to be a mirror of the ecology of the region. in this study, the genetic structure and diversity of sheep breeds from saudi arabia (harri, najdi, naemi, arb, and rufidi) and awassi sheep from jordan as an out-group were investigated using 19 microsatellites. all the breeds had high intra-population genetic diversity expressed as allelic number (7.33) and richness (2.9) and, expected heterozygosity (0.77). structur ... | 2018 | 30319690 |
| determinants of blood water δ18o variation in a population of experimental sheep: implications for paleoclimate reconstruction. | mammalian body, blood and hard tissue oxygen isotope compositions (δ18o values) reflect environmental water and food sources, climate, and physiological processes. for this reason, fossil and archaeological hard tissues, which originally formed in equilibrium with body chemistry, are a valuable record of past climate, landscape paleoecology, and animal physiology and behavior. however, the environmental and physiological determinants of blood oxygen isotope composition have not been determined e ... | 2018 | 30504966 |
| sarcocystis species in wild and domestic sheep (ovis ammon and ovis aries) from china. | sarcocystis species are intracellular protozoan parasites that can pose a threat to animal health and food safety. the aim of this study was to investigate the prevalence of infection with sarcocystis infection in sheep from china. | 2018 | 30509255 |
| apelin system in mammary gland of sheep reared in semi-natural pastures of the central apennines. | sheep are the most bred species in the central italy apennine using the natural pastures as a trophic resource and grazing activity is fundamental to maintain the grassland biodiversity: this goal can be reached only ensuring an economical sustainability to the farmers. this study aimed to investigate the apelin/apelin receptor system in ovine mammary gland and to evaluate the differences induced by food supplementation, in order to shed light on this system function. a flock of 15 comisana x ap ... | 2018 | 30486490 |
| respiratory pathogens and their association with population performance in montana and wyoming bighorn sheep populations. | respiratory disease caused by mycoplasma ovipneumoniae and pasteurellaceae poses a formidable challenge for bighorn sheep (ovis canadensis) conservation. all-age epizootics can cause 10-90% mortality and are typically followed by multiple years of enzootic disease in lambs that hinders post-epizootic recovery of populations. the relative frequencies at which these epizootics are caused by the introduction of novel pathogens or expression of historic pathogens that have become resident in the pop ... | 2018 | 30475861 |
| unpacking multi-trophic herbivore-grass-endophyte interactions: feedbacks across different scales in vegetation responses to soay sheep herbivory. | grazing can induce changes in both plant productivity and nutritional quality, which may subsequently influence herbivore carrying capacity. while research on soay sheep (ovis aries l.) dynamics on hirta island in the st. kilda archipelago has elucidated the complexity of population drivers, including parasites, the role of herbivore-generated feedbacks as an intrinsic regulating factor remains unclear. the sheep lack large predators and every 3-9 years undergo population crashes (overcompensato ... | 2018 | 30460621 |
| diversity of indigenous sheep of an isolated population. | because of the influence of genetics on animal production and the risk of losing genetic diversity of naturally adapted breeds, this study evaluated the genetic diversity of sheep of the morada nova breed belonging to an animal science institute in brazil. the herd in question is one of the country's most representative of the breed. samples of dna extracted from the plasma of 61 animals were used for later analysis of the genotypes using microsatellite molecular markers. | 2018 | 30445945 |
| determination of hematological and serum biochemical reference values for indigenous sheep (ovies aries) in dhaka and chittagong districts of bangladesh. | the study was aimed to determine the reference values of most commonly used hematological and biochemical parameters of indigenous sheep, reared under semi-intensive backyard farms in dhaka and chittagong district, bangladesh. | 2018 | 30250368 |
| shifts in ovine cardiopulmonary microrna expression in late gestation and the perinatal period. | micrornas (mirnas) have been identified as important contributors to the regulation of early fetal cardiopulmonary development. however, mirna expression profiles during late gestation and the early neonatal period are not fully elaborated in large mammals such as sheep (ovis aries). the aim of this study was to sequence mirna from cardiopulmonary tissues in late gestation and neonate sheep to identify changes in mirna expression. | 2018 | 30231073 |
| angiopoietin expression in ovine corpora lutea during the luteal phase: effects of nutrition, arginine and follicle stimulating hormone. | the aim of this study was to evaluate angiopoietin (angpt) 1 and 2, and tyrosine-protein kinase receptor 2 (tie2) expression in the corpora lutea (cl) of fsh-treated, or non-treated sheep administered arginine (arg) or vehicle (saline, sal), and fed a control (c), excess (o) or restricted (u) diet. ewes from each dietary group were treated with arg or sal (experiment 1), and with fsh (experiment 2). luteal tissues were collected at the early-, mid- and/or late-luteal phases of the estrous cycle. ... | 2018 | 30195024 |
| morphometric development of sheep (ovis aries) lymph nodes in fetal period. | immune system plays crucial role in body and lymph nodes are essential parts of this system for combating pathogens. however, no study has ever been conducted on morphometric development of sheep lymph nodes in fetal period. thus, this study attempted to examine the morphometric characteristics of a number of important lymph nodes of some lymphocenters of sheep during fetal period. to this end, 60 pieces of sheep fetuses collected from ahvaz slaughterhouse were fixated in 10% formalin and then d ... | 2018 | 30065800 |
| influence of omega-3 incorporation in sperm preservation medium on physical and kinematic properties of chilled and cryopreserved ram spermatozoa. | two experiments were carried out to investigate the efficiency of supplementing sperm preservation medium with omega-3 polyunsaturated fatty acids on improving liquid-chilled storage and cryopreservation capacity of ram spermatozoa. ejaculates (n = 100) were collected from five adult rams, ovis aries, by an artificial vagina twice weekly throughout the period february-april, 2017. after initial evaluation, ejaculates of each collection session from the same males were pooled, diluted (1:10) with ... | 2018 | 30054952 |
| cloning and differential expression analyses of cdc42 from sheep. | serological diagnosis of brucellosis is still a great challenge due to the infeasibility of discriminating infected animals from vaccinated ones, so it is necessary to search for diagnostic biomarkers for differential diagnosis of brucellosis. | 2018 | 29978136 |
| evaluation of glycoprotein ov8 as a potential antigen for an ovhv-2-specific diagnostic assay. | gammaherpesviruses in the genus macavirus establish clinically unapparent persistent infections in reservoir species. transmission of some of these viruses, including alcelaphine herpesvirus 1 (alhv-1) and ovine herpesvirus 2 (ovhv-2), to clinically susceptible species in the order artiodactyla can result in malignant catarrhal fever (mcf), a usually fatal lymphoproliferative disease. serology can be used to identify mcf virus (mcfv)-infected carrier animals. however, all current serological ass ... | 2018 | 29966004 |
| expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep. | melatonin is an important factor involved in regulating reproduction; it is synthesized enzymatically by the sequential action of melatonin-synthesizing enzymes, arylalkylamine n-acetyltransferase (aanat) and hydroxyindole-o-methyltransferase (hiomt), and exerts its biological functions mainly through receptor-mediated action. to evaluate the expression of melatonin, two melatonin-synthesizing enzymes (hiomt and aanat), and membrane receptors (mt1 and mt2) in oestrous corpus luteum (cl) and cl v ... | 2018 | 29943511 |
| efficacy of administered mesenchymal stem cells in the initiation and co-ordination of repair processes by resident disc cells in an ovine (ovis aries) large destabilizing lesion model of experimental disc degeneration. | forty percent of low back pain cases are due to intervertebral disc degeneration (ivdd), with mesenchymal stem cells (mscs) a reported treatment. we utilized an ovine ivdd model and intradiscal heterologous mscs to determine therapeutic efficacy at different stages of ivdd. | 2018 | 31463452 |
| orf virus infection in alaskan mountain goats, dall's sheep, muskoxen, caribou and sitka black-tailed deer. | the zoonotic orf virus (orfv; genus parapoxvirus, poxviridae family) occurs worldwide and is transmitted between sheep and goats, wildlife and man. archived tissue samples from 16 alaskan wildlife cases, representing mountain goat (oreamnos americanus, n = 8), dall's sheep (ovis dalli dalli, n = 3), muskox (ovibos moschatus, n = 3), sitka black-tailed deer (odocoileus hemionus sitkensis, n = 1) and caribou (rangifer tarandus granti, n = 1), were analyzed. | 2018 | 29467004 |
| linkage disequilibrium in brazilian santa inês breed, ovis aries. | for genomic selection to be successful, there must be sufficient linkage disequilibrium between the markers and the causal mutations. the objectives of this study were to evaluate the extent of ld in ovine using the santa inês breed and to infer the minimum number of markers required to reach reasonable prediction accuracy. in total, 38,168 snps and 395 samples were used. the mean ld between adjacent marker pairs measured by r2 and |d'| were 0.166 and 0.617, respectively. ld values between adjac ... | 2018 | 29892085 |
| isolation and enrichment of putative spermatogonial stem cells from ram (ovis aries) testis. | the present study aimed to isolate and enrich putative sscs from ram testes, which are positive for promyelocytic leukaemia zinc-finger protein (plzf). the putative sscs were isolated using a combination of enzymes with different concentrations, collagenase (1 and 2 mg/ml), hyaluronidase (1 mg/ml) and trypsin (0.25 and 0.5 mg/ml). the isolated sscs were purified using an extracellular matrix such as laminin (20 μg/ml), dsa-lectin (5 μg/ml) and gelatin (0.2%) in combination with bsa (0.5 mg/ml). ... | 2018 | 29861343 |
| contrasting patterns of genomic diversity reveal accelerated genetic drift but reduced directional selection on x-chromosome in wild and domestic sheep species. | analyses of genomic diversity along the x chromosome and of its correlation with autosomal diversity can facilitate understanding of evolutionary forces in shaping sex-linked genomic architecture. strong selective sweeps and accelerated genetic drift on the x-chromosome have been inferred in primates and other model species, but no such insight has yet been gained in domestic animals compared with their wild relatives. here, we analyzed x-chromosome variability in a large ovine data set, includi ... | 2018 | 29790980 |
| microstructure and mechanical properties of different keratinous horns. | animal horns play an important role during intraspecific combat. this work investigates the microstructure and mechanical properties of horns from four representative ruminant species: the bighorn sheep (ovis canadensis), domestic sheep (ovis aries), mountain goat (oreamnos americanus) and pronghorn (antilocapra americana), aiming to understand the relation between evolved microstructures and mechanical properties. microstructural similarity is found where disc-shaped keratin cells attach edge-t ... | 2018 | 29875283 |
| molecular signature of aluminum hydroxide adjuvant in ovine pbmcs by integrated mrna and microrna transcriptome sequencing. | there have been few in vivo studies on the effect of aluminum hydroxide adjuvant and its influence on the immune response to vaccination. in this study, lambs received a parallel subcutaneous treatment with either commercial vaccines containing aluminum hydroxide or an equivalent dose of this compound only with the aim of identifying the activated molecular signature. blood samples were taken from each animal at the beginning and at the end of the experiment and pbmcs isolated. total rna and mir ... | 2018 | 30405610 |
| the ability of magnetic field sensors to monitor feeding in three domestic herbivores. | the rate at which animals ingest food is a fundamental part of animal ecology although it is rarely quantified, with recently-developed animal-attached tags providing a potentially viable approach. however, to date, these methods lack clarity in differentiating various eating behaviours, such as 'chewing' from 'biting'. the aims of this study were to examine the use of inter-mandibular angle sensors (imasens), to quantify grazing behaviour in herbivores including cattle (bos taurus), sheep (ovis ... | 2018 | 30225163 |
| beta-hydroxybutyrate infusion identifies acutely differentially expressed genes related to metabolism and reproduction in the hypothalamus and pituitary of castrated male sheep. | to identify molecular pathways that couple metabolic imbalances and reproduction, we randomly assigned 10 castrated male sheep to be centrally injected into the lateral ventricle through intracerebroventricular cannulas with 1 ml of β-hydroxybutyric acid sodium salt solution (bhb; 12,800 µmol/l) or saline solution (con; 0.9% nacl). approximately 2 h postinjection, sheep were humanely euthanized, and hypothalamus and pituitary tissues were harvested for transcriptome characterization by rna seque ... | 2018 | 29625019 |
| genome-wide variation, candidate regions and genes associated with fat deposition and tail morphology in ethiopian indigenous sheep. | variations in body weight and in the distribution of body fat are associated with feed availability, thermoregulation, and energy reserve. ethiopia is characterized by distinct agro-ecological and human ethnic farmer diversity of ancient origin, which have impacted on the variation of its indigenous livestock. here, we investigate autosomal genome-wide profiles of 11 ethiopian indigenous sheep populations using the illumina ovine 50 k snp beadchip assay. sheep from the caribbean, europe, middle ... | 2018 | 30687385 |
| ameliorating the adverse cardiorespiratory effects of chemical immobilization by inducing general anaesthesia in sheep and goats: implications for physiological studies of large wild mammals. | chemical immobilization is necessary for the physiological study of large wild animals. however, the immobilizing drugs can adversely affect the cardiovascular and respiratory systems, yielding data that do not accurately represent the normal, resting state. we hypothesize that these adverse effects can be ameliorated by reversing the immobilizing agent while holding the animal under general anaesthesia. we used habituated sheep ovis aries (n = 5, 46.9 ± 5.3 kg body mass, mean ± sem) and goats c ... | 2018 | 30232543 |
| surface temperature of ewes during estrous cycle measured by infrared thermography. | the use of infrared thermography has not been used previously to assist sheep reproduction. the aim of this study was evaluate the viability of infrared thermography to identify body surface temperature patterns during ewes' estrous cycle. the estrous cycles of 20 santa ines ewes were synchronized through hormone treatment (progesterone implant). the ewes were submitted to measurements of body temperature (rectal and vaginal) with a digital thermometer and surface temperature of the anus, vulva, ... | 2018 | 30059884 |
| genome-wide snp analysis unveils genetic structure and phylogeographic history of snow sheep (ovis nivicola) populations inhabiting the verkhoyansk mountains and momsky ridge (northeastern siberia). | insights into the genetic characteristics of a species provide important information for wildlife conservation programs. here, we used the ovinesnp50 beadchip developed for domestic sheep to examine population structure and evaluate genetic diversity of snow sheep (ovis nivicola) inhabiting verkhoyansk range and momsky ridge. a total of 1,121 polymorphic snps were used to test 80 specimens representing five populations, including four populations of the verkhoyansk mountain chain: kharaulakh rid ... | 2018 | 30250679 |
| mechanical adaptation of trabecular bone morphology in the mammalian mandible. | alveolar bone, together with the underlying trabecular bone, fulfils an important role in providing structural support against masticatory forces. diseases such as osteoporosis or periodontitis cause alveolar bone resorption which weakens this structural support and is a major cause of tooth loss. however, the functional relationship between alveolar bone remodelling within the molar region and masticatory forces is not well understood. this study investigated this relationship by comparing mamm ... | 2018 | 29740057 |
| light and scanning electron microscopy of the olfactory mucosa in the rufous sengi (elephantulus rufescens). | sengis are eutherian insectivores belonging to superorder afrotheria, a recently defined clade of mammals that diverged from other placentals over 100 million years ago. in this study, a histological and ultrastructural analysis was carried out on the olfactory mucosa (om) of the rufous sengi (elephantulus rufescens) and the data were compared with those reported earlier in the dog (canis familiaris) and the sheep (ovis aries), whose dietary lifestyles are carnivorous and herbivorous, respective ... | 2018 | 29460316 |
| association of textile industry effluent with mutagenicity and its toxic health implications upon acute and sub-chronic exposure. | complex industrial discharges pose certain risks to the ecosystem. this study was aimed at identifying acute and sub-chronic toxicological effects of the textile industry wastewater. the textile wastewater was evaluated for the metals and organic pollutants by atomic absorption spectrophotometer and gc-ms respectively. in vitro genotoxicity and mutagenicity were assessed by comet assay in peripheral lymphocytes isolated from ovis aries and ames test in salmonella typhimurium strains ta-100 and 1 ... | 2018 | 29492685 |
| clusters of dcx+ cells "trapped" in the subcortical white matter of early postnatal cetartiodactyla (tursiops truncatus, stenella coeruloalba and ovis aries). | the cytoskeletal protein doublecortin (dcx) is a marker for neuronal cells retaining high potential for structural plasticity, originating from both embryonic and adult neurogenic processes. some of these cells have been described in the subcortical white matter of neonatal and postnatal mammals. in mice and humans it has been shown they are young neurons migrating through the white matter after birth, reaching the cortex in a sort of protracted neurogenesis. here we show that dcx+ cells in the ... | 2018 | 29980931 |
| the first case of diarrhoea in tibetan sheep, ovis aries, caused by balantidium coli in the qinghai tibetan plateau area, china. | this study was carried out to determine the pathogen-causing diarrhoea in sheep ovis aries in the qinghai tibetan plateau area, china. a trophozoite was identified as species of ciliate alveolates infecting the sheep based on morphological characteristics examined by microscope. it was mostly spherical, colourless and transparent, with many vesicles. macronucleus and contractile vacuoles could not be distinguished. size of the trophozoite was 80-180 × 70-150 μm and its surface was covered with c ... | 2018 | 30630282 |
| how grazing affects soil quality of soils formed in the glaciated northeastern united states. | historically, much of the new england landscape was converted to pasture for grazing animals and harvesting hay. both consumer demand for local sustainably produced food, and the number of small farms is increasing in ri, highlighting the importance of characterizing the effects livestock have on the quality of pasture soils. to assess how livestock affect pasture on charlton and canton soils series in ri, we examined soil quality in farms raising beef cattle (bos taurus), sheep (ovis aries), an ... | 2018 | 29468318 |
| declining home range area predicts reduced late-life survival in two wild ungulate populations. | demographic senescence is increasingly recognised as an important force shaping the dynamics of wild vertebrate populations. however, our understanding of the processes that underpin these declines in survival and fertility in old age remains limited. evidence for age-related changes in foraging behaviour and habitat use is emerging from wild vertebrate studies, but the extent to which these are driven by within-individual changes, and the consequences for fitness, remain unclear. using longitud ... | 2018 | 29656580 |
| morphological variations of the interatrial septum in ovine heart. | smooth septum interatrial septum, patent foramen ovale (pfo) channel and atrial septal pouches (sps) are commonly described variants in humans. recent discoveries on the clinical significance of left-sided sp may encourage the creation of new strategies and devices for the management of sps. however, these strategies may first be tested in the ovine model before implementation in humans. unfortunately, little is known about the presence of sps in ovine. in this study a total of 60 ovine (ovis ar ... | 2018 | 30566482 |
| cloning, identification, and functional analysis of bone marrow stromal cell antigen-2 from sika deer (cervus nippon). | bst-2(tetherin/cd317/hm1.24) has been identified as a cellular antiviral factor that inhibits the release of a wide range of enveloped viruses from infected cells. orthologs of bst-2 have been identified in several species including humans, monkeys, cows, sheep, pigs, and mice. in this study, we cloned the gene and characterized the protein of the bst-2 homolog from sika deer (cervus nippon). cnbst-2 shares 37.8% and 74.2% identity with the bst-2 homologs from homo sapiens and ovis aries, respec ... | 2018 | 29621585 |
| occurrence of blastocystis sp. and pentatrichomonas hominis in sheep and goats in china. | global data regarding the molecular epidemiology of blastocystis sp. and pentatrichomonas hominis in sheep and goats are sparse. china has one of the largest sheep and goat populations in the world. in this study we investigated the occurrence of blastocystis sp. and p. hominis in domestic sheep and goats in china, and analyzed the genetic characterization of these two parasite species. | 2018 | 29454366 |
| den site selection by male brown bears at the population's expansion front. | brown bears (ursus arctos) spend about half of the year in winter dens. in order to preserve energy, bears may select denning locations that minimize temperature loss and human disturbance. in expanding animal populations, demographic structure and individual behavior at the expansion front can differ from core areas. we conducted a non-invasive study of male brown bear den sites at the male-biased, low-density western expansion front of the scandinavian brown bear population, comparing den loca ... | 2018 | 30161161 |
| a fast and reliable real-time pcr method for detection of ten animal species in meat products. | species substitution in meat products is a common problem reported worldwide. this type of food fraud is, typically, an intentional act for economic gain, using sources of low-priced meats in high-value meat products. consequences include economic, health, and religious concerns. highly sensitive and efficient techniques are thus required to detect meat species. this paper describes a method based on real-time pcr to detect 10 animal species (bos taurus, sus scrofa, ovis aries, capra hircus, gal ... | 2018 | 29377112 |
| tear production, intraocular pressure and conjunctival bacterial flora in selected captive wild ruminants. | evaluation of tear production (schirmer's tear test, stt) and measurement of intraocular pressure (iop) were performed in a population of captive wild ungulates in a slovenian zoo during routine annual health check. | 2018 | 28493440 |
| effects of high condensed-tannin substrate, prior dietary tannin exposure, antimicrobial inclusion, and animal species on fermentation parameters following a 48-h in vitro incubation. | condensed tannins (ct), prior dietary ct exposure, animal species, and antimicrobial inclusion effects on 48-h extent of in vitro fermentation were measured in an experiment with a 3 × 2 × 2 × 3 factorial arrangement of treatments. treatments included: species of inoculum donor (bos taurus, ovis aries, or capra hircus; n = 3/species); prior adaptation to dietary ct (not adapted or adapted); culture substrate (low-ct or high-ct); and antimicrobial additive (none, bacterial suppression with penici ... | 2018 | 29365124 |
| infectious keratoconjunctivitis in semi-domesticated eurasian tundra reindeer (rangifer tarandus tarandus): microbiological study of clinically affected and unaffected animals with special reference to cervid herpesvirus 2. | infectious keratoconjunctivitis (ikc) is one of the most common ocular diseases in ruminants worldwide. in addition to keratitis and conjunctivitis, animals with ikc can develop uveitis, corneal ulcer, and in severe cases, blindness. the bacteria moraxella spp. has been described as the primary causative agent of infectious bovine keratoconjunctivitis (ibk) in cattle (bos taurus), while chlamydia spp. and mycoplasma conjunctivae are considered the main causative agents of ikc in sheep (ovis arie ... | 2018 | 29338721 |
| metabolic effects of fecb gene on follicular fluid and ovarian vein serum in sheep (ovis aries). | thefecbgene has been discovered as an important gene in sheep for its high relationship with the ovulation rate, but its regulatory mechanism remains unknown. in the present study, liquid chromatography-mass spectrometry (lc-ms) and gas chromatography-mass spectrometry (gc-ms) techniques were adopted to detect the metabolic effects offecbgene in follicular fluid (ff) and ovarian vein serum (ovs) in small tail han (sth) sheep. anova and random forest statistical methods were employed for the iden ... | 2018 | 29439449 |
| first report of taenia ovis infection in danish sheep (ovis aries). | we report taenia ovis infection in danish sheep for the first time. in spring 2016, the metacestode stage of t. ovis was at slaughter observed in heart muscles, diaphragm and skeletal muscles from approx. a third of all sheep from one specific farm localised in south jutland. the diagnosis was confirmed by molecular typing of the mitochondrial cytochrome c oxidase i (cox1) gene. three newly imported dogs were suspected but the definitive host was unidentifiable. the finding is not regulated in t ... | 2018 | 29426473 |
| complete mitogenomes from kurdistani sheep: abundant centromeric nuclear copies representing diverse ancestors. | the geographical centre of domestication and species diversity for sheep (ovis aries) lies around the kurdistan region of northern iraq, within the 'fertile crescent'. from whole genome sequence reads, we assembled the mitochondrial genomes (mtdna or mitogenome) of five animals of the two main kurdistani sheep breeds hamdani and karadi and found they fitted into known sheep haplogroups (or matrilineages), with some snps. haplotyping 31 animals showed presence of the main asian (hpga) and europea ... | 2018 | 29385875 |
| faecal glucocorticoid metabolites and body temperature in australian merino ewes (ovis aries) during summer artificial insemination (ai) program. | reproductive wastage is a key issue for sheep producers, both regionally and globally. the reproductive output of farm animals can be influenced by physiological and environmental factors. rapid and reliable quantification of physiological stress can provide a useful tool for designing and testing on-farm management interventions to improve farm animal welfare and productivity. in this study, we quantified physiological stress non-invasively using faecal glucocorticoid metabolites-fgms analysis ... | 2018 | 29381759 |
| integrated ovarian mrna and mirna transcriptome profiling characterizes the genetic basis of prolificacy traits in sheep (ovis aries). | the highly prolific breeds of domestic sheep (ovis aries) are globally valuable genetic resources for sheep industry. genetic, nutritional and other environmental factors affect prolificacy traits in sheep. to improve our knowledge of the sheep prolificacy traits, we conducted mrna-mirna integrated profiling of ovarian tissues from two pure breeds with large (finnsheep) vs. small (texel) litter sizes and their f1 crosses, half of which were fed a flushing diet. | 2018 | 29378514 |
| genomic diversity and population structure of three autochthonous greek sheep breeds assessed with genome-wide dna arrays. | in the present study, genome-wide genotyping was applied to characterize the genetic diversity and population structure of three autochthonous greek breeds: boutsko, karagouniko and chios. dairy sheep are among the most significant livestock species in greece numbering approximately 9 million animals which are characterized by large phenotypic variation and reared under various farming systems. a total of 96 animals were genotyped with the illumina's ovinesnp50k microarray beadchip, to study the ... | 2018 | 29372305 |
| expression and cellular localization of double sex and mab-3 related transcription factor 1 in testes of postnatal small-tail han sheep at different developmental stages. | double sex and mab-3 related transcription factor 1 (dmrt1), an evolutionarily conserved gene, is a sex-related gene expressed in male gonads, that is involved in the regulation of sex differentiation, testicular development and reproductive function maintenance. until now, functional studies on the dmrt1 gene in sheep (ovis aries) have been lacking. in this study, testis, heart, liver, spleen, lung, kidney and longissimus dorsi muscle tissues were collected from small-tail han sheep at 0, 2, 5, ... | 2018 | 29174386 |
| l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters crat and prdx1 expression. | l-carnitine is an antioxidant and β-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. however, few studies have evaluated its beneficial effects in embryos produced in vivo. this study aimed to evaluate the effect of l-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6-7 in vivo-produced ovine embryos. l-carnitine (3.72 mm) was added to vitrification (experiment 1) or war ... | 2018 | 28965027 |
| dynamics of social behaviour at parturition in a gregarious ungulate. | group living is the behavioural response that results when individuals assess the costs vs benefits of sociality, and these trade-offs vary across an animal's life. here we quantitatively assess how periparturient condition (mother/non-mother) and births affect the dynamics of social interactions of a gregarious ungulate, and how such can help to explain evolutionary hypotheses of the mother-offspring bond. to achieve this we used data of the individual movement of a group of scottish blackface ... | 2018 | 29360558 |
| revealing the selection history of adaptive loci using genome-wide scans for selection: an example from domestic sheep. | one of the approaches to detect genetics variants affecting fitness traits is to identify their surrounding genomic signatures of past selection. with established methods for detecting selection signatures and the current and future availability of large datasets, such studies should have the power to not only detect these signatures but also to infer their selective histories. domesticated animals offer a powerful model for these approaches as they adapted rapidly to environmental and human-med ... | 2018 | 29357834 |
| molecular characterization and differential expression analysis of interleukin 1β from ovis aries. | the interleukin-1 family is an important component of the innate immune system and plays an important role in regulating immune responses on the invasion of intracellular parasites in the acquired immune system. interleukin 1β (il-1β) is one of the members of the il-1 family that predominantly activates downstream signaling pathways to play immunological functions of stimulating t and b lymphocyte activation and promoting the various syntheses of inflammatory substances in conjunction with other ... | 2018 | 29331367 |
| detection of bovine deltapapillomavirus dna in peripheral blood of healthy sheep (ovis aries). | blood samples from 65 sheep were tested for the presence of bovine deltapapillomavirus (δpvs) dna. the sheep were divided into three groups. sheep in groups 1 and 2 were from sardinia and campania, respectively, and were in contact with cattle and grazed on lands contaminated with bracken fern. sheep in group 3 lived in closed pens and had no contact with cattle. these sheep were fed hay that did not contain bracken fern. bovine δpv e5 dna was detected in blood from 24 of 27 (89%) sheep in group ... | 2018 | 29330926 |
| proteomic analyses of ram (ovis aries) testis during different developmental stages. | male reproductive capacity is essential for animal breeding and reproduction. in males, the testes produce sperm and secrete androgen, processes which require precise regulation by multiple proteins. the composition of proteins in the ram testes has not yet been studied systematically, thus, the application of proteomics to explore differential protein regulation during ram testes development is of great significance. in the present study, ram testes were studied at five different developmental ... | 2018 | 29279200 |
| purification, structural and biophysical characterisation of the major seminal plasma protein from texel rams. | spermadhesins are a group of low molecular weight proteins present in seminal plasma. in texel rams, they represent more than 70% of the seminal plasma proteins. although their functions have not yet been fully clarified, there is much discussion about the role of these proteins in maintaining sperm viability during and after the semen freezing process. this work sought to isolate the major component of the seminal plasma from rams of the texel breed (o. aries spd2) and to evaluate its structura ... | 2018 | 29249516 |
| in-gel and offgel-based proteomic approach for authentication of meat species from minced meat and meat products. | fraudulent mislabelling of processed meat products on a global scale that cannot be detected using conventional techniques necessitates sensitive, robust and accurate methods of meat authentication to ensure food safety and public health. in the present study, we developed an in-gel (two-dimensional gel electrophoresis, 2de) and offgel-based proteomic method for authenticating raw and cooked water buffalo (bubalus bubalis), sheep (ovis aries) and goat (caprus hircus) meat and their mixes. | 2018 | 28737240 |
| whole genome sequencing of hulunbuir short-tailed sheep for identifying candidate genes related to the short-tail phenotype. | the hulunbuir short-tailed sheep (ovis aries) is a breed native to china, in which the short-tail phenotype is the result of artificial and natural selection favoring a specific set of genetic mutations. here, we analyzed the genetic differences between short-tail and normal-tail phenotypes at the genomic level. selection signals were identified in genome-wide sequences. from 16 sheep, we identified 72,101,346 single nucleotide polymorphisms. selection signals were detected based on the fixation ... | 2018 | 29208649 |
| long-term dynamics of mycoplasma conjunctivae at the wildlife-livestock interface in the pyrenees. | functional roles of domestic and wild host populations in infectious keratoconjunctivitis (ikc) epidemiology have been extensively discussed claiming a domestic reservoir for the more susceptible wild hosts, however, based on limited data. with the aim to better assess ikc epidemiology in complex host-pathogen alpine systems, the long-term infectious dynamics and molecular epidemiology of mycoplasma conjunctivae was investigated in all host populations from six study areas in the pyrenees and on ... | 2017 | 29016676 |
| nutritional and metabolic stressors on ovine oocyte development and granulosa cell functions in vitro. | the present study was undertaken to study the effect of ammonia, urea, non-esterified fatty acid (nefa), and β-hydroxybutyric acid (β-ohb) on oocyte development and granulosa cell (gc) growth parameter of ovine (ovis aries). ovine oocytes were matured in vitro in the presence of different concentration of ammonia, urea, nefa, and β-ohb for 24 h, in vitro inseminated and evaluated for cleavage and blastocyst yield. same concentrations of ammonia, urea, nefa, and β-ohb were examined on growth para ... | 2017 | 28986745 |