Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| evolutionary conservation of candidate osmoregulation genes in plant phloem sap-feeding insects. | the high osmotic pressure generated by sugars in plant phloem sap is reduced in phloem-feeding aphids by sugar transformations and facilitated water flux in the gut. the genes mediating these osmoregulatory functions have been identified and validated empirically in the pea aphid acyrthosiphon pisum: sucrase 1 (suc1), a sucrase in glycoside hydrolase family 13 (gh13), and aquaporin 1 (aqp1), a member of the drosophila integral protein (drip) family of aquaporins. here, we describe molecular anal ... | 2016 | 26896054 |
| a jasmonate-inducible defense trait transferred from wild into cultivated tomato establishes increased whitefly resistance and reduced viral disease incidence. | whiteflies damage tomatoes mostly via the viruses they transmit. cultivated tomatoes lack many of the resistances of their wild relatives. in order to increase protection to its major pest, the whitefly bemisia tabaci and its transmitted tomato yellow leaf curl virus (tylcv), we introgressed a trichome-based resistance trait from the wild tomato solanum pimpinellifolium into cultivated tomato, solanum lycopersicum. the tomato backcross line bc5s2 contains acylsucrose-producing type-iv trichomes, ... | 2016 | 27920785 |
| elevated o₃ and tylcv infection reduce the suitability of tomato as a host for the whitefly bemisia tabaci. | the effects of elevated atmospheric ozone (o₃) levels on herbivorous insects have been well studied, but little is known about the combined effects of elevated o₃ and virus infection on herbivorous insect performance. using open-top chambers in the field, we determined the effects of elevated o₃ and tomato yellow leaf curl virus (tylcv) infection on wild-type (wt) tomato and 35s tomato (jasmonic acid (ja) defense-enhanced genotype) in association with whitefly, bemisia tabaci gennadius biotype b ... | 2016 | 27916792 |
| the contrasting effects of elevated co2 on tylcv infection of tomato genotypes with and without the resistance gene, mi-1.2. | elevated atmospheric co2 typically enhances photosynthesis of c3 plants and alters primary and secondary metabolites in plant tissue. by modifying the defensive signaling pathways in host plants, elevated co2 could potentially affect the interactions between plants, viruses, and insects that vector viruses. r gene-mediated resistance in plants represents an efficient and highly specific defense against pathogens and herbivorous insects. the current study determined the effect of elevated co2 on ... | 2016 | 27881989 |
| the whitefly bemisia tabaci knottin-1 gene is implicated in regulating the quantity of tomato yellow leaf curl virus ingested and transmitted by the insect. | the whitefly bemisia tabaci is a major pest to agricultural crops. it transmits begomoviruses, such as tomato yellow leaf curl virus (tylcv), in a circular, persistent fashion. transcriptome analyses revealed that b. tabaci knottin genes were responsive to various stresses. upon ingestion of tomato begomoviruses, two of the four knottin genes were upregulated, knot-1 (with the highest expression) and knot-3. in this study, we examined the involvement of b. tabaci knottin genes in relation to tyl ... | 2016 | 27455309 |
| the salicylic acid-mediated release of plant volatiles affects the host choice of bemisia tabaci. | the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) causes serious crop losses worldwide by transmitting viruses. we have previously shown that salicylic acid (sa)-related plant defenses directly affect whiteflies. in this study, we applied exogenous sa to tomato plants in order to investigate the interaction between sa-induced plant volatiles and nonviruliferous b. tabaci b and q or b- and q-carrying tomato yellow leaf curl virus (tylcv). the results showed that exogenous sa caused ... | 2016 | 27376280 |
| the autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies. | macroautophagy/autophagy plays an important role against pathogen infection in mammals and plants. however, little has been known about the role of autophagy in the interactions of insect vectors with the plant viruses, which they transmit. begomoviruses are a group of single-stranded dna viruses and are exclusively transmitted by the whitefly bemisia tabaci in a circulative manner. in this study, we found that the infection of a begomovirus, tomato yellow leaf curl virus (tylcv) could activate ... | 2016 | 27310765 |
| tomato yellow leaf curl virus (tylcv-il): a seed-transmissible geminivirus in tomatoes. | tomato yellow leaf curl virus (tylcv) is one of the most well-known tomato-infecting begomoviruses and transmitted by bemisia tabaci. seed transmission has previously been reported for some rna viruses, but tylcv has not previously been described as a seed-borne virus. in 2013 and 2014, without whitefly-mediated transmission, tylcv was detected in young tomato plants germinated from fallen fruits produced from tylcv-infected tomato plants in the previous cultivation season. in addition, tylcv-is ... | 2016 | 26743765 |
| tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted by the whitefly bemisia tabaci to tomato and other crops. tylcv proteins are endangered by the host defenses. we have analyzed the capacity of the tomato plant and of the whitefly insect vector to degrade the six proteins encoded by the tylcv genome. tomato and whitefly demonstrated the highest proteolytic activity in the fractions containing soluble proteins, less-in large protein aggregates; a significant decrease of tylcv prot ... | 2016 | 26654789 |
| tomato yellow leaf curl virus differentially influences plant defence responses to a vector and a non-vector herbivore. | plants frequently engage in simultaneous interactions with diverse classes of biotic antagonists. differential induction of plant defence pathways by these antagonists, and interactions between pathways, can have important ecological implications; however, these effects are currently not well understood. we explored how tomato yellow leaf curl virus (tylcv) influenced the performance of its vector (bemisia tabaci) and a non-vector herbivore (tetranychus urticae) occurring separately or together ... | 2016 | 26436779 |
| the non-canonical tomato yellow leaf curl virus recombinant that displaced its parental viruses in southern morocco exhibits a high selective advantage in experimental conditions. | recombination events are frequently inferred from the increasing number of sequenced viral genomes, but their impact on natural viral populations has rarely been evidenced. tylcv-is76 is a recombinant (begomovirus,geminiviridae) between the israel strain of tomato yellow leaf curl virus (tylcv-il) and the spanish strain of tomato yellow leaf curl sardinia virus (tylcsv-es) that was generated most probably in the late 1990s in southern morocco (souss). its emergence in the 2000s coincided with th ... | 2016 | 27902403 |
| rna interference of an antimicrobial peptide, btdef, reduces tomato yellow leaf curl china virus accumulation in the whitefly bemisia tabaci. | the whitefly bemisia tabaci (gennadius) is considered one of the main pests for agriculture. one important problem with the whitefly is its notorious status as a vector for plant viruses, primarily begomoviruses. we have previously identified a defensin-like antimicrobial peptide, btdef, from the whitefly b. tabaci meam1. however, the function of btdef in the immune system of the insect vector and begomovirus transmission has yet to be explored. | 2016 | 27804213 |
| sequencing and comparison of the rickettsia genomes from the whitefly bemisia tabaci middle east asia minor i. | the whitefly, bemisia tabaci, harbors the primary symbiont 'candidatus portiera aleyrodidarum' and a variety of secondary symbionts. among these secondary symbionts, rickettsia is the only one that can be detected both inside and outside the bacteriomes. infection with rickettsia has been reported to influence several aspects of the whitefly biology, such as fitness, sex ratio, virus transmission and resistance to pesticides. however, mechanisms underlying these differences remain unclear, large ... | 2016 | 27273750 |
| effects of isaria fumosorosea on tylcv (tomato yellow leaf curl virus) accumulation and transmitting capacity of bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is transmitted by the bemisia tabaci pest middle east-asia minor 1 (meam1) in china. isaria fumosorosea is a fungal pathogen of b. tabaci. however, the effects of fungal infection on tylcv expression and transmission by meam1 are unclear. in this study, potted tomatoes containing second instar nymphs of meam1 were treated with i. fumosorosea ifb01 strain and the relationship between fungal infection in meam1 and its tylcv transmission capacity was investigat ... | 2016 | 27716852 |
| recombinant fungal entomopathogen rnai target insect gene. | rna interference (rnai) technology is considered as an alternative for control of pests. however, rnai has not been used in field conditions yet, since delivering exogenous ds/sirna to target pests is very difficult. the laboratory methods of introducing the ds/sirna into insects through feeding, micro feeding / dripping and injecting cannot be used in fields. transgenic crop is perhaps the most effective application of rnai for pest control, but it needs long-time basic researches in order to r ... | 2016 | 27715447 |
| a bemisia tabaci midgut protein interacts with begomoviruses and plays a role in virus transmission. | begomoviruses are a major group of plant viruses, transmitted exclusively by bemisia tabaci (gennadius) in a persistent circulative non-propagative manner. the information regarding molecular and cellular basis underlying begomovirus - whitefly interaction is very scarce. evidences have suggested that the insect gut possesses some crucial protein receptors that allow specific entry of virus into the insect haemolymph. we have performed yeast two hybrid gut cdna expression library screening again ... | 2016 | 26488331 |
| use of posttranscription gene silencing in squash to induce resistance against the egyptian isolate of the squash leaf curl virus. | squash leaf curl virus (sqlcv) is a bipartite begomovirus affecting squash plants. it is transmitted by whitefly bemisia tabaci biotype b causing severe leaf curling, vein banding, and molting ending by stunting. in this study full-length genomic clone of sqlcv egyptian isolated and posttranscriptional gene silencing (ptgs) has been induced to develop virus resistance. the noubaria sqlcv has more than 95% homology with jordon, israel, lebanon, palestine, and cairo isolates. two genes fragment fr ... | 2016 | 27034922 |
| transmission of squash vein yellowing virus to and from cucurbit weeds and effects on sweetpotato whitefly (hemiptera: aleyrodidae) behavior. | since 2003, growers of florida watermelon [citrullus lanatus (thunb.) matsum. and nakai] have periodically suffered large losses from a disease caused by squash vein yellowing virus (sqvyv), which is transmitted by the whitefly middle east-asia minor 1 (meam1), formerly bemisia tabaci (gennadius) biotype b. common cucurbit weeds like balsam apple (momordica charantia l.) and smellmelon [cucumis melo var. dudaim (l.) naud.] are natural hosts of sqvyv, and creeping cucumber (melothria pendula l.) ... | 2016 | 27400705 |
| infectivity, effects on helper viruses and whitefly transmission of the deltasatellites associated with sweepoviruses (genus begomovirus, family geminiviridae). | begomoviruses (family geminiviridae) are whitefly-transmitted viruses with single-stranded dna genomes that are frequently associated with dna satellites. these satellites include non-coding satellites, for which the name deltasatellites has been proposed. although the first deltasatellite was identified in the late 1990s, little is known about the effects they have on infections of their helper begomoviruses. recently a group of deltasatellites were identified associated with sweepoviruses, a g ... | 2016 | 27453359 |
| antioxidant enzyme responses induced by whiteflies in tobacco plants in defense against aphids: catalase may play a dominant role. | bemisia tabaci meam1 (middle east-asia minor 1) feeding alters antioxidative enzyme activity in some plant species. infestation of b. tabaci nymphs decreases myzus persicae performance on systemic, but not local leaves of tobacco plants. however, it is unclear if b. tabaci nymphs induced antioxidant activities contributing to the aphid resistance. | 2016 | 27788203 |
| bensulfuron-methyl treatment of soil affects the infestation of whitefly, aphid, and tobacco mosaic virus on nicotiana tabacum. | bensulfuron-methyl (bsm) is widely used in paddy soil for weed control. bsm residue in the soil has been known to inhibit the growth of sensitive crop plants. however, it is unknown whether bsm residue can affect the agrosystem in general. in this study, we have found significant effects of bsm on the infestation of bemisia tabaci, myzus persicae, and tobacco mosaic virus (tmv) in nicotiana tabacum. the soil was treated with bsm before the pest inoculation. the herbicide-treated tobaccos showed ... | 2016 | 28083007 |
| tomato infection by whitefly-transmitted circulative and non-circulative viruses induce contrasting changes in plant volatiles and vector behaviour. | virus infection frequently modifies plant phenotypes, leading to changes in behaviour and performance of their insect vectors in a way that transmission is enhanced, although this may not always be the case. here, we investigated bemisia tabaci response to tomato plants infected by tomato chlorosis virus (tocv), a non-circulative-transmitted crinivirus, and tomato severe rugose virus (tosrv), a circulative-transmitted begomovirus. moreover, we examined the role of visual and olfactory cues in ho ... | 2016 | 27529271 |
| effects of foliar and systemic insecticides on whitefly transmission and incidence of cucurbit yellow stunting disorder virus. | cucurbit yellow stunting disorder virus (cysdv) is a cosmopolitan viral disease transmitted by bemisia tabaci that infects cucurbit crops. cantaloupe production in the southwestern usa has been confronted by epidemics of cysdv since 2006 when it was first identified in arizona and california. as a phloem-limited virus that is vectored in a semi-persistent manner by b. tabaci, cysdv has transmission characteristics that may be suppressed by select insecticide applications. | 2016 | 27862891 |
| the draft genome of whitefly bemisia tabaci meam1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance. | the whitefly bemisia tabaci (hemiptera: aleyrodidae) is among the 100 worst invasive species in the world. as one of the most important crop pests and virus vectors, b. tabaci causes substantial crop losses and poses a serious threat to global food security. | 2016 | 27974049 |
| effects of host plant factors on the bacterial communities associated with two whitefly sibling species. | although discrepancy in the specific traits and ecological characteristics of bemisia tabaci between species are partially attributed to the b. tabaci-associated bacteria, the factors that affect the diversity of b. tabaci-associated bacteria are not well-understood. we used the metagenomic approach to characterize the b. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria. | 2016 | 27008327 |
| infectious clones of the crinivirus cucurbit chlorotic yellows virus are competent for plant systemic infection and vector transmission. | cucurbit chlorotic yellows virus (ccyv), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. ccyv is naturally transmitted only by whitefly bemisia tabaci. here we constructed full-length cdna clones of ccyv (rna1 and rna2) fused to the t7 rna polymerase promoter and the cauliflower mosaic virus 35s promoter. ccyv replicated and accumulated efficiently in cucumis sativus protoplasts transfected with in vitro transcripts. without rna2, rna1 replicated efficientl ... | 2016 | 26982585 |
| cellular and molecular remodelling of a host cell for vertical transmission of bacterial symbionts. | various insects require intracellular bacteria that are restricted to specialized cells (bacteriocytes) and are transmitted vertically via the female ovary, but the transmission mechanisms are obscure. we hypothesized that, in the whitefly bemisia tabaci, where intact bacteriocytes (and not isolated bacteria) are transferred to oocytes, the transmission mechanism would be evident as cellular and molecular differences between the nymph (pre-adult) and adult bacteriocytes. we demonstrate dramatic ... | 2016 | 27358364 |
| members of wrky group iii transcription factors are important in tylcv defense signaling pathway in tomato (solanum lycopersicum). | transmitted by the whitefly bemisia tabaci, tomato yellow leaf curly virus (tylcv) has posed serious threats to plant growth and development. plant innate immune systems against various threats involve wrky group iii transcription factors (tfs). this group participates as a major component of biological processes in plants. | 2016 | 27717312 |
| a transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters. | the first generation transgenic crops used strong constitutive promoters for transgene expression. however, tissue-specific expression is desirable for more precise targeting of transgenes. moreover, piercing/sucking insects, which are generally resistant to insecticidal bacillus thuringiensis (bt) proteins, have emerged as a major pests since the introduction of transgenic crops expressing these toxins. phloem-specific promoters isolated from banana bunchy top virus (bbtv) were used for the exp ... | 2016 | 27708374 |
| a peptidoglycan recognition protein acts in whitefly (bemisia tabaci) immunity and involves in begomovirus acquisition. | peptidoglycan recognition proteins (pgrps) are multifunctional pattern recognition proteins. here, we report that a pgrp gene, btpgrp, encodes a pgrp from the whitefly bemisia tabaci (meam1) that binds and kills bacteria in vitro. we analyzed btpgrp transcriptional profiling, and the distribution of the cognate protein within the midgut. fungal infection and wasp parasitization induced expression of btpgrp. silencing btpgrp with artificial media amended with dsrna led to reduced expression of a ... | 2016 | 27892529 |
| deep sequencing-based transcriptome analysis reveals the regulatory mechanism of bemisia tabaci (hemiptera: aleyrodidae) nymph parasitized by encarsia sophia (hymenoptera: aphelinidae). | the whitefly bemisia tabaci is a genetically diverse complex with multiple cryptic species, and some are the most destructive invasive pests of many ornamentals and crops worldwide. encarsia sophia is an autoparasitoid wasp that demonstrated high efficiency as bio-control agent of whiteflies. however, the immune mechanism of b. tabaci parasitization by e. sophia is unknown. in order to investigate immune response of b. tabaci to e. sophia parasitization, the transcriptome of e. sophia parasitize ... | 2016 | 27332546 |
| rnai-mediated mortality of the whitefly through transgenic expression of double-stranded rna homologous to acetylcholinesterase and ecdysone receptor in tobacco plants. | the whitefly bemisia tabaci (genn.) is a pest and vector of plant viruses to crop and ornamental plants worldwide. using rna interference (rnai) to down regulate whitefly genes by expressing their homologous double stranded rnas in plants has great potential for management of whiteflies to reduce plant virus disease spread. using a tobacco rattle virus-derived plasmid for in planta transient expression of double stranded rna (dsrna) homologous to the acetylcholinesterase (ache) and ecdysone rece ... | 2016 | 27929123 |
| biotype characterization, developmental profiling, insecticide response and binding property of bemisia tabaci chemosensory proteins: role of csp in insect defense. | chemosensory proteins (csps) are believed to play a key role in the chemosensory process in insects. sequencing genomic dna and rna encoding csp1, csp2 and csp3 in the sweet potato whitefly bemisia tabaci showed strong variation between b and q biotypes. analyzing csp-rna levels showed not only biotype, but also age and developmental stage-specific expression. interestingly, applying neonicotinoid thiamethoxam insecticide using twenty-five different dose/time treatments in b and q young adults s ... | 2016 | 27167733 |
| small rnas from bemisia tabaci are transferred to solanum lycopersicum phloem during feeding. | the phloem-feeding whitefly bemisia tabaci is a serious pest to a broad range of host plants, including many economically important crops such as tomato. these insects serve as a vector for various devastating plant viruses. it is known that whiteflies are capable of manipulating host-defense responses, potentially mediated by effector molecules in the whitefly saliva. we hypothesized that, beside putative effector proteins, small rnas (srna) are delivered by b. tabaci into the phloem, where the ... | 2016 | 27933079 |
| a primary screening and applying of plant volatiles as repellents to control whitefly bemisia tabaci (gennadius) on tomato. | with the goal of finding a new way to reduce population densities of bemisia tabaci biotype q in greenhouses, seven repellent volatile chemicals and their combinations were screened. the mixture of dlco (d-limonene, citral and olive oil (63:7:30)) had a better cost performance(sc50 = 22.59 mg/ml)to repel whiteflies from settling than the other mixtures or single chemicals. in the greenhouse, in both the choice test and the no-choice tests, the number of adult whiteflies that settled on 1% dlco-t ... | 2016 | 26907368 |
| microrna profiling of the whitefly bemisia tabaci middle east-aisa minor i following the acquisition of tomato yellow leaf curl china virus. | the begomoviruses are the largest and most economically important group of plant viruses exclusively vectored by whitefly (bemisia tabaci) in a circulative, persistent manner. during this process, begomoviruses and whitefly vectors have developed close relationships and complex interactions. however, the molecular mechanisms underlying these interactions remain largely unknown, and the microrna profiles for viruliferous and nonviruliferous whiteflies have not been studied. | 2016 | 26837429 |
| tomato yellow leaf curl virus: no evidence for replication in the insect vector bemisia tabaci. | begomovirus ssdna plant virus (family geminiviridae) replication within the bemisia tabaci vector is controversial. transovarial transmission, alteration to whitefly biology, or detection of viral transcripts in the vector are proposed as indirect evidence of replication of tomato yellow leaf curl virus (tylcv). recently, contrasting direct evidence has been reported regarding the capacity of tylcv to replicate within individuals of b. tabaci based on quantitave pcr approaches. time-course exper ... | 2016 | 27476582 |
| implication of the whitefly bemisia tabaci cyclophilin b protein in the transmission of tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv) is a single-stranded (ssdna) begomoviruses that causes severe damage to tomato and several other crops worldwide. tylcv is exclusively transmitted by the sweetpotato whitefly, bemisia tabaci in a persistent circulative and propagative manner. previous studies have shown that the transmission, retention, and circulation of tylcv in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a prot ... | 2016 | 27895657 |
| deciphering the biology of deltasatellites from the new world: maintenance by new world begomoviruses and whitefly transmission. | deltasatellites are small noncoding dna satellites associated with begomoviruses. the study presented here has investigated the biology of two deltasatellites found in wild malvaceous plants in the new world (nw). infectious clones of two nw deltasatellites (from malvastrum coromandelianum and sidastrum micranthum) and associated begomoviruses were constructed. infectivity in nicotiana benthamiana and their natural malvaceous hosts was assessed. the nw deltasatellites were not able to spread aut ... | 2016 | 27400152 |
| high ozone (o3) affects the fitness associated with the microbial composition and abundance of q biotype bemisia tabaci. | ozone (o3) affects the fitness of an insect, such as its development, reproduction and protection against fungal pathogens, but the mechanism by which it does so remains unclear. here, we compared the fitness (i.e., the growth and development time, reproduction and protection against beauveria bassiana (b. bassiana) of q biotype whiteflies fumigated under ho3 (280 ± 20 ppb) and control o3 (50 ± 10 ppb) concentrations. moreover, we determined that gene expression was related to development, repro ... | 2016 | 27799921 |
| survey of indigenous entomopathogenic fungi and evaluation of their pathogenicity against the carmine spider mite, tetranychus cinnabarinus (boisd.), and the whitefly, bemisia tabaci (genn.) biotype b. | the carmine spider mite, tetranychus cinnabarinus, and the silverleaf whitefly, bemisia tabaci, are serious pests of both field- and greenhouse-grown crops in south-western turkey. control of these pests has been heavily dependent upon chemical pesticides. the objectives of this study were to investigate the occurrence of indigenous entomopathogenic fungi (epf) in field populations of t. cinnabarinus and b. tabaci, and to evaluate their pathogenicity against these pests. for this purpose, a surv ... | 2016 | 26929052 |
| how to start with a clean crop: biopesticide dips reduce populations of bemisia tabaci (hemiptera: aleyrodidae) on greenhouse poinsettia propagative cuttings. | (1) global movement of propagative plant material is a major pathway for introduction of bemisia tabaci (hemiptera: aleyrodidae) into poinsettia greenhouses. starting a poinsettia crop with high pest numbers disrupts otherwise successful biological control programs and widespread resistance of b. tabaci against pesticides is limiting growers' options to control this pest; (2) this study investigated the use of several biopesticides (mineral oil, insecticidal soap, beauveria bassiana, isaria fumo ... | 2016 | 27681741 |
| improved shelf life of dried beauveria bassiana blastospores using convective drying and active packaging processes. | the yeast form (blastospore) of the dimorphic insect-pathogenic fungus beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. in this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of b. bassiana e ... | 2016 | 27198727 |
| tri-tek (petroleum horticultural oil) and beauveria bassiana: use in eradication strategies for bemisia tabaci mediterranean species in uk glasshouses. | the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a pest of global importance on both outdoor and glasshouse crops. to date, b. tabaci has not become established in the uk. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. mediterranean species is now the most prevalent bemisia species entering the uk. increasing neonicotinoid resistance is becoming increasingly widesp ... | 2015 | 26463071 |
| glucose concentration alters dissolved oxygen levels in liquid cultures of beauveria bassiana and affects formation and bioefficacy of blastospores. | the filamentous fungus beauveria bassiana is an economically important pathogen of numerous arthropod pests and is able to grow in submerged culture as filaments (mycelia) or as budding yeast-like blastospores. in this study, we evaluated the effect of dissolved oxygen and high glucose concentrations on blastospore production by submerged cultures of two isolates of b. bassiana, esalq1432 and gha. results showed that maintaining adequate dissolved oxygen levels coupled with high glucose concentr ... | 2015 | 25947245 |
| liquid culture fermentation for rapid production of desiccation tolerant blastospores of beauveria bassiana and isaria fumosorosea strains. | a major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. previous attempts in producing beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8days) and produced cells that had poor survival after desiccation and storage. in this study, isolates of beauveria bassiana and isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and ... | 2015 | 25497914 |
| estimation of the whitefly bemisia tabaci genome size based on k-mer and flow cytometric analyses. | whiteflies of the bemisia tabaci (hemiptera: aleyrodidae) cryptic species complex are among the most important agricultural insect pests in the world. these phloem-feeding insects can colonize over 1000 species of plants worldwide and inflict severe economic losses to crops, mainly through the transmission of pathogenic viruses. surprisingly, there is very little genomic information about whiteflies. as a starting point to genome sequencing, we report a new estimation of the genome size of the b ... | 2015 | 26463411 |
| flow cytometry and k-mer analysis estimates of the genome sizes of bemisia tabaci b and q (hemiptera: aleyrodidae). | the genome sizes of the b- and q-types of the whitefly bemisia tabaci (gennnadius) were estimated using flow cytometry (drosophila melanogaster as the dna reference standard and propidium iodide (pi) as the fluorochrome) and k-mer analysis. for flow cytometry, the mean nuclear dna content was 0.686 pg for b-type males, 1.392 pg for b-type females, 0.680 pg for q-type males, and 1.306 pg for q-type females. based on the relationship between dna content and genome size (1 pg dna = 980 mbp), the ha ... | 2015 | 26042041 |
| only a minority of broad-range detoxification genes respond to a variety of phytotoxins in generalist bemisia tabaci species. | generalist insect can utilize two different modes for regulating their detoxification genes, the constitutive mode and the induced mode. here, we used the bemisia tabaci sibling species meam1 and med, as a model system for studying constitutive and induced detoxification resistance and their associated tradeoffs. b. tabaci adults were allowed to feed through membranes for 24 h on diet containing only sucrose or sucrose with various phytotoxins. quantitative real-time pcr analyses of 18 detoxific ... | 2015 | 26655836 |
| transcription analysis of neonicotinoid resistance in mediterranean (med) populations of b. tabaci reveal novel cytochrome p450s, but no nachr mutations associated with the phenotype. | bemisia tabaci is one of the most damaging agricultural pests world-wide. although its control is based on insecticides, b. tabaci has developed resistance against almost all classes of insecticides, including neonicotinoids. | 2015 | 26573457 |
| rapid accumulation and low degradation: key parameters of tomato yellow leaf curl virus persistence in its insect vector bemisia tabaci. | of worldwide economic importance, tomato yellow leaf curl virus (tylcv, begomovirus) is responsible for one of the most devastating plant diseases in warm and temperate regions. the dna begomoviruses (geminiviridae) are transmitted by the whitefly species complex bemisia tabaci. although geminiviruses have long been described as circulative non-propagative viruses, observations such as long persistence of tylcv in b. tabaci raised the question of their possible replication in the vector. we moni ... | 2015 | 26625871 |
| metabolic coevolution in the bacterial symbiosis of whiteflies and related plant sap-feeding insects. | genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. this study of the whitefly bemisia tabaci and two bacteria (portiera aleyrodidarum and hamiltonella defensa) cohoused in each host cell investigated whether the decay of portiera metabolism genes is complemented by host and hamiltonella genes, and compared the metabolic traits of the whitefly symbiosis with other sap-feeding insects (aphids, psyllids, and mealybugs). paral ... | 2015 | 26377567 |
| differential temporal changes of primary and secondary bacterial symbionts and whitefly host fitness following antibiotic treatments. | where multiple symbionts coexist in the same host, the selective elimination of a specific symbiont may enable the roles of a given symbiont to be investigated. we treated the mediterranean species of the whitefly bemisia tabaci complex by oral delivery of the antibiotic rifampicin, and then examined the temporal changes of its primary symbiont "candidatus portiera aleyrodidarum" and secondary symbiont "ca. hamiltonella defensa" as well as host fitness for three generations. in adults treated wi ... | 2015 | 26510682 |
| whitefly genome expression reveals host-symbiont interaction in amino acid biosynthesis. | whitefly (bemisia tabaci) complex is a serious insect pest of several crop plants worldwide. it comprises several morphologically indistinguishable species, however very little is known about their genetic divergence and biosynthetic pathways. in the present study, we performed transcriptome sequencing of asia 1 species of b. tabaci complex and analyzed the interaction of host-symbiont genes in amino acid biosynthetic pathways. | 2015 | 26000976 |
| genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly bemisia tabaci. | the whitefly bemisia tabaci is an important agricultural pest with global distribution. this phloem-sap feeder harbors a primary symbiont, "candidatus portiera aleyrodidarum", which compensates for the deficient nutritional composition of its food sources, and a variety of secondary symbionts. interestingly, all of these secondary symbionts are found in co-localization with the primary symbiont within the same bacteriocytes, which should favor the evolution of strong interactions between symbion ... | 2015 | 25887812 |
| reduction of viral load in whitefly (bemisia tabaci gen.) feeding on rnai-mediated bean golden mosaic virus resistant transgenic bean plants. | the rnai concept was explored to silence the rep gene from the bean golden mosaic virus (bgmv) and a genetically modified (gm) bean immune to the virus was previously generated. we investigated if bgmv-viruliferous whiteflies would reduce viral amount after feeding on gm plants. bgmv dna amount was significantly reduced in whiteflies feeding in gm-plants (compared with insects feeding on non-gm plants) for a period of 4 and 8 days in 52% and 84% respectively. | 2015 | 26297125 |
| control of insect vectors and plant viruses in protected crops by novel pyrethroid-treated nets. | long-lasting insecticide-treated nets (llitns) constitute a novel alternative that combines physical and chemical tactics to prevent insect access and the spread of insect-transmitted plant viruses in protected enclosures. this approach is based on a slow-release insecticide-treated net with large hole sizes that allow improved ventilation of greenhouses. the efficacy of a wide range of llitns was tested under laboratory conditions against myzus persicae, aphis gossypii and bemisia tabaci. two n ... | 2015 | 25404196 |
| comparison of transmission of papaya leaf curl china virus among four cryptic species of the whitefly bemisia tabaci complex. | begomoviruses are transmitted by cryptic species of the whitefly bemisia tabaci complex, often in a species-specific manner. papaya leaf curl china virus (palcucnv) has been recorded to infect several crops including papaya, tomato and tobacco in china. to help assess the risks of spread of this virus, we compared the acquisition, retention and transmission of palcucnv among four species of whiteflies, middle east-asia minor 1 (meam1), mediterranean (med), asia 1 and asia ii 7. all four species ... | 2015 | 26486606 |
| pathogenicity of isaria fumosorosea to bemisia tabaci, with some observations on the fungal infection process and host immune response. | isaria fumosorosea is an important pathogen of whiteflies, and is used as a mycoinsecticide against this pest in many regions of the world. we quantified the pathogenicity of the chinese isolate if-1106 against different life stages of sweetpotato whitefly, bemisia tabaci, on cucumber plants, and describe the infection process and aspects of the host immunological response in the laboratory. the second instar was the most susceptible life stage to infection, with mortality rates at 10(7)conidia/ ... | 2015 | 26264671 |
| expression of dsrna in recombinant isaria fumosorosea strain targets the tlr7 gene in bemisia tabaci. | rna interference (rnai) technology shows a great potential in controlling agricultural pests, despite the difficulty of introducing exogenous dsrna/sirna into target pests. isaria fumosorosea is a common fungal pathogen of the b-biotype bemisia tabaci (whitefly), which is a widespread pest. entomopathogenic fungi directly penetrate the cuticle and invade insect hemocoel. application of i. fumosorosea expressing dsrna of whitefly immunity-related gene may aid in developing rnai technology to effe ... | 2015 | 26198409 |
| identification and characterization of two phospholipid hydroperoxide glutathione peroxidase genes from the mediterranean species of the whitefly bemisia tabaci complex. | phospholipid hydroperoxide glutathione peroxidases (phgpxs) are essential enzymes of the cellular antioxidant defense system during insect-plant interactions. however, little attention has been devoted to the functional characterization of phgxps in the whitefly bemisia tabaci. here, we report the identification and characterization of two phgpx genes, designated as btq-phgpx1 and btq-phgpx2 from the mediterranean species of the b. tabaci complex. sequence analysis indicated that the length of b ... | 2015 | 25639712 |
| control of sweet potato virus diseases. | sweet potato (ipomoea batatas) is ranked seventh in global food crop production and is the third most important root crop after potato and cassava. sweet potatoes are vegetative propagated from vines, root slips (sprouts), or tubers. therefore, virus diseases can be a major constrain, reducing yields markedly, often more than 50%. the main viruses worldwide are sweet potato feathery mottle virus (spfmv) and sweet potato chlorotic stunt virus (spcsv). effects on yields by spfmv or spcsv alone are ... | 2015 | 25591876 |
| differential profiles of direct and indirect modification of vector feeding behaviour by a plant virus. | plant viruses interact with their insect vectors directly and indirectly via host plants, and this tripartite interaction may produce fitness benefits to both the vectors and the viruses. our previous studies show that the middle east-asia minor 1 (meam1) species of the whitefly bemisia tabaci complex improved its performance on tobacco plants infected by the tomato yellow leaf curl china virus (tylccnv), which it transmits, although virus infection of the whitefly per se reduced its performance ... | 2015 | 25567524 |
| real-time pcr protocols for the quantification of the begomovirus tomato yellow leaf curl sardinia virus in tomato plants and in its insect vector. | tomato yellow leaf curl sardinia virus (tylcsv) (geminiviridae) is an important pathogen, transmitted by the whitefly bemisia tabaci, that severely affects the tomato production in the mediterranean basin. here, we describe real-time pcr protocols suitable for relative and absolute quantification of tylcsv in tomato plants and in whitefly extracts. using primers and probe specifically designed for tylcsv, the protocols for relative quantification allow to compare the amount of tylcsv present in ... | 2015 | 25287496 |
| evaluating weeds as hosts of tomato yellow leaf curl virus. | bemisia tabaci (gennadius) biotype b transmits tomato yellow leaf curl virus (tylcv), which affects tomato production globally. prompt destruction of virus reservoirs is a key component of virus management. identification of weed hosts of tylcv will be useful for reducing such reservoirs. the status of weeds as alternate hosts of tylcv in florida remains unclear. in greenhouse studies, b. tabaci adults from a colony reared on tylcv-infected tomato were established in cages containing one of four ... | 2015 | 26314055 |
| replication of tomato yellow leaf curl virus in its whitefly vector, bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted exclusively by the whitefly bemisia tabaci in a persistent, circulative manner. replication of tylcv in its vector remains controversial, and thus far, the virus has been considered to be nonpropagative. following 8 h of acquisition on tylcv-infected tomato plants or purified virions and then transfer to non-tylcv-host cotton plants, the amounts of virus inside whitefly adults significantly increased (>2-fold) during the first fe ... | 2015 | 26178995 |
| transmission of tomato yellow leaf curl virus by bemisia tabaci as affected by whitefly sex and biotype. | bemisia tabaci is a serious pest of vegetables and other crops worldwide. the most damaging and predominant b. tabaci biotypes are b and q, and both are vectors of tomato yellow leaf curl virus (tylcv). previous research has shown that q outperforms b in many respects but comparative research is lacking on the ability of b and q to transmit viruses. in the present study, we tested the hypothesis that b and q differ in their ability to transmit tylcv and that this difference helps explain tylcv o ... | 2015 | 26021483 |
| recessive resistance derived from tomato cv. tyking-limits drastically the spread of tomato yellow leaf curl virus. | the tomato yellow leaf curl disease (tylcd) causes severe damage to tomato (solanum lycopersicum l.) crops throughout tropical and subtropical regions of the world. tylcd is associated with a complex of single-stranded circular dna plant viruses of the genus begomovirus (family geminiviridae) transmitted by the whitefy bemisia tabaci gennadius (hemiptera: aleyrodidae). the tomato inbred line tx 468-rg is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. tyki ... | 2015 | 26008699 |
| temporal effects of a begomovirus infection and host plant resistance on the preference and development of an insect vector, bemisia tabaci, and implications for epidemics. | persistent plant viruses, by altering phenotypic and physiological traits of their hosts, could modulate the host preference and fitness of hemipteran vectors. a majority of such modulations increase vector preference for virus-infected plants and improve vector fitness, ultimately favouring virus spread. nevertheless, it remains unclear how these virus-induced modulations on vectors vary temporally, and whether host resistance to the pathogen influences such effects. this study addressed the tw ... | 2015 | 26529402 |
| effectiveness of cyantraniliprole for managing bemisia tabaci (hemiptera: aleyrodidae) and interfering with transmission of tomato yellow leaf curl virus on tomato. | cyantraniliprole is the second xylem-systemic active ingredient in the new anthranilic diamide class. greenhouse (2006), growth chamber (2007), and field studies (2009-2010) were conducted to determine the efficacy of cyantraniliprole for managing bemisia tabaci (gennadius) biotype b and in interfering with transmission of tomato yellow leaf curl virus (tylcv) by this whitefly. cyantraniliprole applied as soil treatments (200 sc) or foliar sprays (100 od) provided excellent adult whitefly contro ... | 2015 | 26470209 |
| manipulation of host quality and defense by a plant virus improves performance of whitefly vectors. | pathogen-mediated interactions between insect vectors and their host plants can affect herbivore fitness and the epidemiology of plant diseases. while the role of plant quality and defense in mediating these tripartite interactions has been recognized, there are many ecologically and economically important cases where the nature of the interaction has yet to be characterized. the bemisia tabaci (gennadius) cryptic species mediterranean (med) is an important vector of tomato yellow leaf curl viru ... | 2015 | 26470098 |
| two host clades, two bacterial arsenals: evolution through gene losses in facultative endosymbionts. | bacterial endosymbiosis is an important evolutionary process in insects, which can harbor both obligate and facultative symbionts. the evolution of these symbionts is driven by evolutionary convergence, and they exhibit among the tiniest genomes in prokaryotes. the large host spectrum of facultative symbionts and the high diversity of strategies they use to infect new hosts probably impact the evolution of their genome and explain why they undergo less severe genomic erosion than obligate symbio ... | 2015 | 25714744 |
| host selection by the autoparasitoid encarsia pergandiella on primary (bemisia tabaci) and secondary (eretmocerus mundus) hosts. | in autoparasitoids, females are generally primary endoparasitoids of hemiptera, while males are hyperparasitoids developing in or on conspecific females or other primary parasitoids. female-host acceptance can be influenced by extrinsic and/or intrinsic factors. in this paper, we are concerned with intrinsic factors such as nutritional status, mating status, etc. we observed the behavior of encarsia pergandiella howard (hymenoptera: aphelinidae) females when parasitizing primary (3rd instar larv ... | 2015 | 24992443 |
| first report of bemisia tabaci mediterranean (q biotype) species in brazil. | the whitefly bemisia tabaci is a major cosmopolitan pest and comprises a complex of more than 36 cryptic species that cause serious damage to agricultural crops worldwide. in this study, the mediterranean species of b. tabaci, formerly known as q biotype, was identified for the first time in brazil. | 2015 | 25212515 |
| rna interference of the p450 cyp6cm1 gene has different efficacy in b and q biotypes of bemisia tabaci. | cytochrome p450 monooxygenases have been proven to be associated with high resistance in bemisia tabaci b biotype (middle east-asia minor 1 genetic group) and q biotype (mediterranean genetic group) to the neonicotinoid class of insecticides. in this study, the rna interference (rnai) effects on p450 cyp6cm1 gene expression, mortality and pesticide-detoxifying ability between b. tabaci b and q biotypes were compared in an attempt to provide a basis for potential rnai application in management of ... | 2015 | 25200527 |
| species and endosymbiont diversity of bemisia tabaci (homoptera: aleyrodidae) on vegetable crops in senegal. | bemisia tabaci-transmitted geminiviruses are one of the major threats on cassava and vegetable crops in africa. however, to date, few studies are available on the diversity of b. tabaci and their associated endosymbionts in africa. more than 28 species have been described in the complex of b. tabaci cryptic species; among them, 2 are invasive pests worldwide: med and meam1. in order to assess the species diversity of b. tabaci in vegetable crops in senegal, several samplings in different localit ... | 2015 | 24789572 |
| detection of genetically isolated entities within the mediterranean species of bemisia tabaci: new insights into the systematics of this worldwide pest. | the taxonomy of the species complex bemisia tabaci, a serious agricultural pest worldwide, is not well resolved yet, even though species delimitation is critical for designing effective control strategies. based on a threshold of 3.5% mitochondrial (mtcoi) sequence divergence, recent studies have identified 28 putative species. among them, mitochondrial variability associated with particular symbiotic compositions (=cytotypes) can be observed, as in med, which raises the question of whether it i ... | 2015 | 24863547 |
| movement of cyantraniliprole in plants after foliar applications and its impact on the control of sucking and chewing insects. | given the physical properties of insecticides, there is often some movement of these compounds within crop plants following foliar application. in this context, movement of two formulations of cyantraniliprole, an anthranilic diamide, was characterized for translocation to new growth, distribution within a leaf and penetration through the leaf cuticle. | 2015 | 24771486 |
| amblyseius swirskii: what made this predatory mite such a successful biocontrol agent? | the predatory mite amblyseius swirskii quickly became one of the most successful biocontrol agents in protected cultivation after its introduction into the market in 2005 and is now released in more than 50 countries. there are several key factors contributing to this success: (1) it can control several major pests including the western flower thrips, frankliniella occidentalis, the whiteflies bemisia tabaci and trialeurodes vaporariorum and the broad mite, polyphagotarsonemus latus, simultaneou ... | 2015 | 25524511 |
| non-invasive delivery of dsgst is lethal to the sweet potato whitefly, bemisia tabaci (g.) (hemiptera: aleyrodidae). | the sweet potato whitefly, bemisia tabaci (g.) biotype b (hemiptera: aleyrodidae), is one of the most economically important pest, by being a dreaded vector of geminiviruses, and also causes direct damage to the crops by sucking phloem sap. glutathione s-transferase (gst) is a large family of multifunctional enzymes that play pivotal roles in the detoxification of secondary allelochemical produced by the host plants and in insecticide resistance, thus regulates insect growth and development. the ... | 2015 | 25480347 |
| optimal strategies for interception, detection, and eradication in plant biosecurity. | the introduction of invasive species causes damages from the economic and ecological point of view. interception of plant pests and eradication of the established populations are two management options to prevent or limit the risk posed by an invasive species. management options generate costs related to the interception at the point of entry, and the detection and eradication of established field populations. risk managers have to decide how to allocate resources between interception, field det ... | 2015 | 25263711 |
| cross-resistance and baseline susceptibility of mediterranean strains of bemisia tabaci to cyantraniliprole. | the whitefly bemisia tabaci gennadius is a severe pest in many field and greenhouse crops worldwide and has developed resistance to insecticides from most chemical classes. the ease with which this pest develops resistance makes it essential to incorporate new compounds with different modes of action and no cross-resistance with those previously used into insecticide resistance management strategies. to that end, the systemic effect of the new diamide cyantraniliprole was tested with multiresist ... | 2015 | 25138719 |
| foraging behaviour of the parasitoid eretmocerus eremicus under intraguild predation risk by macrolophus pygmaeus. | intraguild predation (igp), predation between species that use a common resource, can affect the populations of a pest, of the pest's natural enemy (ig prey) and of the predator of the pest's natural enemy (ig predator). in this study, we determined whether the parasitoid eretmocerus eremicus (hymenoptera: aphelinidae) (ig prey), modifies its foraging behaviour under the risk of igp by macrolophus pygmaeus (hemiptera: miridae) (ig predator). parasitoid behaviour was analysed using two bioassays ... | 2015 | 25377901 |
| dynamics of the endosymbiont rickettsia in an insect pest. | a new heritable bacterial association can bring a fresh set of molecular capabilities, providing an insect host with an almost instantaneous genome extension. increasingly acknowledged as agents of rapid evolution, inherited microbes remain underappreciated players in pest management programs. a rickettsia bacterium was tracked sweeping through populations of an invasive whitefly provisionally described as the "b" or "meam1" of the bemisia tabaci species complex, in the southwestern usa. in this ... | 2015 | 25626393 |
| the dynamics and environmental influence on interactions between cassava brown streak disease and the whitefly, bemisia tabaci. | cassava brown streak disease (cbsd) is currently the most significant virus disease phenomenon affecting african agriculture. in this study, we report results from the most extensive set of field data so far presented for cbsd in africa. from assessments of 515 farmers' plantings of cassava, incidence in the coastal zone of tanzania (46.5% of plants; 87% of fields affected) was higher than in the lake zone (22%; 34%), but incidences for both zones were greater than previous published records. th ... | 2015 | 25585059 |
| potential of two populations of amblyseius swirskii (acari: phytoseiidae) for the control of bemisia tabaci biotype b (hemiptera: aleyrodidae) in brazil. | predatory mites of the family phytoseiidae are commercialized in european and north american countries for the control of whiteflies (insecta: aleyrodidae). amblyseius swirskii athias-henriot (acari: phytoseiidae) is one of the predators used for that purpose. this predator is not found in brazil and in many other countries, but its introduction could promote biological control of bemisia tabaci (gennadius) biotype b (silverleaf whitefly) in those countries. the aim of this study was to compare ... | 2015 | 26387112 |
| feeding of whitefly on tobacco decreases aphid performance via increased salicylate signaling. | the feeding of bemisia tabaci nymphs trigger the sa pathway in some plant species. a previous study showed that b. tabaci nymphs induced defense against aphids (myzus persicae) in tobacco. however, the mechanism underlying this defense response is not well understood. | 2015 | 26381273 |
| [the effects of transgenic cry1ac+cry2ab cotton on cotton bollworm control and functional response of predators on whitefly]. | in this study, we detected and clarified the roles of transgenic cry1ac+cry2ab cotton "639020" in controlling cotton bollworm (helicoverpa armigera) during critical periods of bud stage (second generation of bollworm), flowering stage (third generation of bollworm) and bolling stage (fourth generation of bollworm) as well as the influences of 639020 cotton on functional response of the main predators (chrysopa sinica larvae, propylaea japonica, orius and erigonidium graminicola ) on whitefly usi ... | 2015 | 26351054 |
| aphid-induced defences in chilli affect preferences of the whitefly, bemisia tabaci (hemiptera: aleyrodidae). | the sweetpotato whitefly (wf), bemisia tabaci, is a major pest that damages a wide range of vegetable crops in malaysia. wf infestation is influenced by a variety of factors, including previous infestation of the host plant by other insect pests. this study investigated the effects of previous infestation of host chilli plants by the green peach aphid (myzus persicae) on the olfactory behavioural response of b. tabaci, using free-choice bioassay with a y-tube olfactometer. we analysed volatile o ... | 2015 | 26334135 |
| distribution of bemisia tabaci genetic groups in india. | the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a phloem-feeding, economically important pest of crops worldwide. in addition to direct damage, it also vectors a number of plant viruses belonging to the family geminiviridae. its populations differ biologically with respect to insecticide resistance, virus transmission and host range. therefore, understanding genetic variation among populations is important for management. we sequenced 850 bp of the mitochondrial coi (mtcoi) g ... | 2015 | 26314072 |
| generating within-plant spatial distributions of an insect herbivore based on aggregation patterns and per-node infestation probabilities. | most predator-prey models extrapolate functional responses from small-scale experiments assuming spatially uniform within-plant predator-prey interactions. however, some predators focus their search in certain plant regions, and herbivores tend to select leaves to balance their nutrient uptake and exposure to plant defenses. individual-based models that account for heterogeneous within-plant predator-prey interactions can be used to scale-up functional responses, but they would require the gener ... | 2015 | 26313173 |
| infection of bacterial endosymbionts in insects: a comparative study of two techniques viz pcr and fish for detection and localization of symbionts in whitefly, bemisia tabaci. | bacterial endosymbionts have been associated with arthropods and large number of the insect species show interaction with such bacteria. different approaches have been used to understand such symbiont- host interactions. the whitefly, bemisia tabaci, a highly invasive agricultural pest, harbors as many as seven different bacterial endosymbionts. these bacterial endosymbionts are known to provide various nutritional, physiological, environmental and evolutionary benefits to its insect host. in th ... | 2015 | 26287997 |
| differential expression patterns among heat-shock protein genes and thermal responses in the whitefly bemisia tabaci (meam 1). | there is convincing evidence that heat-shock proteins (hsp) are upregulated by stress conditions in insects; however, the relative contribution of each hsp gene to the heat-shock response remains unclear. here we considered the whitefly bemisia tabaci (meam 1), a phloem feeder and invasive species whose molecular stress response is an important mechanism for overcoming heat stress. we assessed the expression of the hsp23, 40, 70 and 90 genes at the mrna level when submitted to heat shocks of 40 ... | 2015 | 26267515 |
| persistent, circulative transmission of begomoviruses by whitefly vectors. | begomoviruses comprise an emerging and economically important group of plant viruses exclusively transmitted by the sweetpotato whitefly bemisia tabaci in many regions of the world. the past twenty years have witnessed significant progress in studying the molecular interactions between members of this virus group and b. tabaci. mechanisms and proteins encoded by the insect vector and its bacterial symbionts, which have been shown to be important for virus transmission, have been identified and t ... | 2015 | 26196230 |
| flupyradifurone (sivanto™) and its novel butenolide pharmacophore: structural considerations. | flupyradifurone (4-[(2,2-difluoroethyl)amino]-2(5h)-furanone), a member of the new class of butenolide insecticides, contains a novel bioactive scaffold as pharmacophore. it is very versatile in terms of application methods to a variety of crops, exhibits excellent and fast action against a broad spectrum of sucking pest insects including selected neonicotinoid resistant pest populations such as whiteflies and aphids expressing metabolic resistance mechanisms. as a partial agonist flupyradifuron ... | 2015 | 26047109 |
| development of a lateral flow test to detect metabolic resistance in bemisia tabaci mediated by cyp6cm1, a cytochrome p450 with broad spectrum catalytic efficiency. | cotton whitefly, bemisia tabaci (genn.) (homoptera: aleyrodidae) is a major sucking pest in many agricultural and horticultural cropping systems globally. the frequent use of insecticides of different mode of action classes resulted in populations resisting treatments used to keep numbers under economic damage thresholds. recently it was shown that resistance to neonicotinoids such as imidacloprid is linked to the over-expression of cyp6cm1, a cytochrome p450 monooxygenase detoxifying imidaclopr ... | 2015 | 26047106 |
| transcriptional responses of invasive and indigenous whiteflies to different host plants reveal their disparate capacity of adaptation. | the whitefly bemisia tabaci contains more than 35 cryptic species. the higher adaptability of middle east-asia minor 1 (meam1) cryptic species has been recognized as one important factor for its invasion and displacement of other indigenous species worldwide. here we compared the performance of the invasive meam1 and the indigenous asia ii 3 whitefly species following host plant transfer from a suitable host (cotton) to an unsuitable host (tobacco) and analyzed their transcriptional responses. a ... | 2015 | 26041313 |
| salicylic acid is required for mi-1-mediated resistance of tomato to whitefly bemisia tabaci, but not for basal defense to this insect pest. | plant defense to pests or pathogens involves global changes in gene expression mediated by multiple signaling pathways. a role for the salicylic acid (sa) signaling pathway in mi-1-mediated resistance of tomato (solanum lycopersicum) to aphids was previously identified and its implication in the resistance to root-knot nematodes is controversial, but the importance of sa in basal and mi-1-mediated resistance of tomato to whitefly bemisia tabaci had not been determined. sa levels were measured be ... | 2015 | 26032615 |
| prevalence and genetic diversity of endosymbiotic bacteria infecting cassava whiteflies in africa. | cassava provides over half of the dietary requirement for more than 200 million poor in africa. in recent years, cassava has been affected by an epidemic of a virus disease called cassava brown streak disease (cbsd) that is spreading in much of eastern and central africa, affecting food security and the economic development of the poor. the viruses that cause cbsd are transmitted by the insect vector whitefly (bemisia tabaci), which have increased to very high numbers in some african countries. ... | 2015 | 25933928 |
| detection of cucurbit chlorotic yellows virus from bemisia tabaci captured on sticky traps using reverse transcription loop-mediated isothermal amplification (rt-lamp) and simple template preparation. | cucurbit chlorotic yellows virus (ccyv) of the genus crinivirus within the family closteroviridae is an emerging infectious agent of cucurbits leading to severe disease and significant economic losses. effective detection and identification methods for this virus are urgently required. in this study, a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed to detect ccyv from its vector bemisia tabaci. lamp primer sets to detect ccyv were evaluated for their s ... | 2015 | 25912723 |