Publications

cytochemical investigation of gastric gland component cells with high-pressure freezing followed by freeze-substitution and hydrophilic resin embedding.	gastric gland component cells were electron-microscopically and immunoelectronmicroscopically examined with high-pressure freezing followed by freeze substitution and a low-temperature embedding resin method and compared to that of the conventional chemical-fixation method. the rat gastric gland was high-pressure frozen, freeze-substituted with acetone-containing osmium or acrolein, and embedded in epon 812 or lowicryl k4m, respectively. using the high-pressure freezing method, the vitreous free ...	2002	12418087
conformational analysis of two xylose-containing n-glycans in aqueous solution by using 1h nmr roesy and noesy spectroscopy in combination with md simulations.	the conformational behavior of the synthetic hexa- and heptasaccharide methyl beta-glycosides alpha-d-manp-(1 --> 6)-[alpha-d-manp-(1 --> 3)-][beta-d-xylp-(1 --> 2)-]beta-d-manp-(1 --> 4)-beta-d-glcpnac-(1 --> 4)-beta-d-glcpnac-(1 --> ome and alpha-d-manp-(1 --> 6)-[alpha-d-manp-(1 --> 3)-][beta-d-xylp-(1 --> 2)-]beta-d-manp-(1 --> 4)-beta-d-glcpnac-(1 --> 4)-[alpha-l-fucp-(1 --> 6)-]beta-d-glcpnac-(1 --> ome, representing the xylosylated and the xylosylated alpha-(1 --> 6)-fucosylated core stru ...	2002	12433493
preparation of gc protein-derived macrophage activating factor (gcmaf) and its structural characterization and biological activities.	gc protein has been reported to be a precursor of gc protein-derived macrophage activation factor (gcmaf) in the inflammation-primed macrophage activation cascade. an inducible beta-galactosidase of b cells and neuraminidase of t cells convert gc protein to gcmaf.	2002	12553073
changes in the distribution of sugar receptors in blood vessels and cell elements of rat brain caused by photochemically induced focal ischaemia.	the lectin technique was applied to the histochemical studies of glycoconjugates comprising membranes of capillary endothelial cells and other cellular elements of the cns. in the studies we used the following lectins: concanavalin a (con a), bandeirea simplicifolia agglutinin (bs-i), helix pomatia agglutinin (hpa), ricinus communis agglutinin (rca-120), wheat germ agglutinin (wga). we noticed changes in the localisation of the glycoconjugates, which are the result of cerebral ischaemia caused b ...	2002	12572776
comparative study of blood group-recognizing lectins toward abo blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides.	binding specificities of abo blood group-recognizing lectins toward blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides were studied by lectin-blotting analysis, enzyme linked immunosorbent assay and lectin-conjugated agarose column chromatography. human serum albumin conjugated with a- and b-trisaccharides was clearly recognized by helix pomatia (hpa), phaseolus lunatus, dolichos biflorus agglutinins, and griffonia simplicifolia i agglutinin b(4), respectiv ...	2001	11342253
the detection of psilocin in human urine.	pharmacokinetic studies of psilocybin in humans have shown the rapid dephosphorylation of psilocybin to psilocin with further conversion to 4-hydroxy-tryptophole (4ht) and 4-hydroxyindole-3-acetic acid (4hiaa) in plasma. our study shows that psilocin also undergoes conjugation and can be found in the urine as the psilocin-glucuronide conjugate. recoveries after enzymatic hydrolysis of the urine with beta-glucuronidase (helix pomatia or e. coli) when compared to non-hydrolyzed urine confirmed the ...	2001	11373000
isolation, characterization, and propagation of rat conjunctival goblet cells in vitro.	to isolate, culture, and characterize goblet cells from the conjunctiva of rats.	2001	11381047
lectin binding patterns of uterine glands in mares with chronic endometrial degeneration.	to evaluate changes of glycoconjugate in uterine glands of endometrial tissues obtained from mares.	2001	11400838
galnac glycoprotein expression by breast cell lines, primary breast cancer and normal breast epithelial membrane.	over-expression of n-acetylgalactosamine glycoproteins as detected by binding of the lectin from helix pomatia (hpa), is associated with metastatic competence and poor patient prognosis in a range of human adenocarcinomas. these glycoproteins remain poorly characterised, and their functional role has yet to be elucidated. this study describes characterisation of a range of human breast/breast cancer cell lines for the expression of the n-acetylgalactosaminylated glycoproteins of interest, and th ...	2001	11592774
hydrolysis of conjugated metabolites of buprenorphine. i. the quantitative enzymatic hydrolysis of buprenorphine-3-beta-d-glucuronide in human urine.	buprenorphine, which is a powerful analgesic, a substitution drug for opioids widely used in europe, and a promising new drug currently undergoing clinical trials in the treatment of opioid dependence in the u.s., is excreted in human urine mainly as glucuronide conjugates. in gas chromatographic-mass spectrometric analysis, the urine specimens must be first hydrolyzed to release buprenorphine from its glucuronide conjugates. in order to evaluate the existing hydrolysis methods and to find the o ...	2001	11599605
proteome and glycosylation mapping identifies post-translational modifications associated with aggressive breast cancer.	changes in glycosylation of glycoproteins and glycolipids is a common feature of cancer and may influence cancer cell behaviour, perhaps by enabling cell-cell interactions which favour metastasis or by allowing cancer cells to evade immuno-surveillance. studies to identify glycosylation changes in human cancer have often used immunohistochemical techniques with lectins or antibodies and human tissue sections. whilst some detailed biochemical studies have been performed there are few clinically r ...	2001	11677781
metal concentrations in helix pomatia, helix aspersa and arion rufus: a comparative study.	in this study we evaluated the differences between concentrations of copper, iron, zinc, manganese, lead and cadmium in three terrestrial gastropods: helix pomatia, helix aspersa and arion rufus, collected in a semi-rural location in northern italy. metal concentrations in the foot and in the digestive gland were measured. in the hepatopancreas, copper and zinc did not differ significantly in the three species; the levels of copper were also similar in the foot. in comparison to helix sps., a. r ...	2001	11706793
simultaneous determination of fifteen low-dosed benzodiazepines in human urine by solid-phase extraction and gas chromatography-mass spectrometry.	a gas chromatographic-mass spectrometric method was developed for the simultaneous analysis of 15 low-dosed benzodiazepines, both parent compounds and their corresponding metabolites, in human urine. the target compounds are alprazolam, alpha-hydroxyalprazolam, 4-hydroxyalprazolam, flunitrazepam, 7-aminoflunitrazepam, desmethylflunitrazepam, flurazepam, hydroxyethylflurazepam, nitrogen-desalkylflurazepam, ketazolam, oxazepam, lormetazepam, lorazepam, triazolam and alpha-hydroxytriazolam. nitroge ...	2001	11767312
localized infusion of tunicamycin in rat hemimandibles: alteration of the basal lamina associated with maturation stage ameloblasts.	at the beginning of the maturation stage of amelogenesis, ameloblasts deposit a basal lamina (bl) at the interface between their apical surface and maturing enamel. this structure is rich in glycoconjugates and is proposed to exhibit adhesive and/or filtering functions. to clarify its role, we have applied a recently developed surgical window model to locally administer tunicamycin (tm), an antibiotic that interferes with n-glycosylation, in the rat hemimandible using an osmotic minipump. male w ...	2001	11156685
analysis of glycoconjugate patterns of normal and hormone-induced dysplastic noble rat prostates, and an androgen-independent noble rat prostate tumor, by lectin histochemistry and protein blotting.	alteration of the expression of glycoconjugates is frequently observed in tumors. however, studies on the changes of cellular glycosylation in the early premalignant stage of prostate carcinogenesis are scarce.	2001	11170128
helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer.	predicting long-term outcome after breast-cancer diagnosis remains problematic, particularly for patients with clinically small, axillary lymph node- negative tumours. evidence suggests that the lectin helix pomatia agglutinin (hpa) identifies oligosaccharides associated with poor-prognosis cancer. our aim was to identify oligosaccharides that bind hpa in aggressive breast cancers. breast-cancer cell lines (mcf-7, bt-549 and bt-20) and a cell line from human milk (hbl-100), which showed a range ...	2001	11241316
n-acetylgalactosamine, n-acetylglucosamine and sialic acid expression in primary breast cancers.	binding of the lectin from helix pomatia (hpa), which recognises n-acetylgalactosamine and n-acetylglucosamine glycans, is a predictor of metastasis and poor prognosis in a number of human adenocarcinomas, including breast cancer. the glycoproteins to which it binds in these tumours have been only partially characterised, and the mechanisms underlying their biosynthesis remain unknown. in this study, 111 primary breast cancers were assessed for binding of hpa and labelling characteristics were c ...	2001	11252626
isolation and characterization of an alpha-macroglobulin from the gastropod mollusc helix pomatia with tetrameric structure and preserved activity after methylamine treatment.	a proteinase inhibitor with m(r) 697000 and 20.3% (w/w) carbohydrate was isolated from the haemolymph of the snail helix pomatia and characterized. it was shown to have a tetrameric structure with subunits disulphide linked by two. it inhibited the activity of several types of proteinases against large substrates but not that of trypsin against n-alpha-benzoyl-dl-arginine-4-nitroanilide. this indicated a nonspecific and steric hindrance mode of inhibition. the ratio of trypsin molecules inactiva ...	2001	11342036
lectin histochemistry to detect altered glycosylation in cells and tissues.	a lectin is "a protein or glycoprotein of non-immune origin, not an enzyme, that binds to carbohydrates and agglutinates cells" (1). lectins are naturally occurring substances, most commonly derived from plant or sometimes invertebrate sources, that can be exploited in the laboratory to detect and reveal carbohydrate structures in or on the surface of cells in very much the same way that antibodies can be used to reveal specific antigens. lectins can detect very subtle alterations in cellular gl ...	2001	21340890
kinetics of the bohr effect in the reaction of helix pomatia beta-hemocyanin with oxygen.		2001	29614
immunological and electrophoretic comparison of the haemocyanins of helix pomatia [proceedings].		2001	92959
relaxation kinetics of helix pomatia alpha-hemocyanin. whole-half molecule reaction at ph 5.7 in 0.4 m nacl.		2000	18098
some properties and the axonal transport of choline acetyltransferase in nervous tissue of the snail helix pomatia.		2000	87198
galactan biosynthesis in snails: a comparative study of beta-(1--> 6) galactosyltransferases from helix pomatia and biomphalaria glabrata.	adult snails synthesize in their albumen glands a polysaccharide which is composed exclusively of d- or d- and l-galactose (gal) residues which are interglycosidically linked by 1 --> 3 and 1 --> 6 bonds. it is the only carbohydrate source for embryos and freshly hatched snails. two galactosyltransferases are described in this study which are most likely involved in the biosynthesis of this polysaccharide. one identified in helix pomatia acts on oligosaccharides and could be used to synthesize a ...	2000	11192266
topographic organization of efferent neurons with different neurochemical characters in the cerebral ganglia of the snail helix pomatia.	this study provides a description of the organization of neurons efferent to different head areas in the cerebral ganglia of helix pomatia, revealed by simultaneous ni-lysine and co-lysine back-filling of different pairs of cerebral nerves. the backfills show that labeled cerebral neurons that innervate the head areas are concentrated in seven representation foci distributed in different parts of the cerebral ganglia. almost each head area is represented in each focus. at a gross level, the repr ...	2000	10862109
enzymatic hydrolysis of conjugated steroid metabolites: search for optimum conditions using response surface methodology.	the optimum conditions for hydrolysing conjugated metabolites of steroid hormones in bovine urine were performed with helix pomatia juice, beta-glucuronidase from bovine liver and preparations of limpets and abalone entrails using response surface methodology. the experimental design and empirical modelling used allowed us to assess the main effects of factors (time, temperature, ph and enzyme quantity) and to predict the optimum conditions for each enzyme preparation. confirmatory experiments w ...	2000	11219062
the rational design of semisynthetic peroxidases.	a semisynthetic peroxidase was designed by exploiting the structural similarity of the active sites of vanadium dependent haloperoxidases and acid phosphatases. incorporation of vanadate ion into the active site of phytase (e.c. 3.1.3.8), which mediates in vivo the hydrolysis of phosphate esters, leads to the formation of a semisynthetic peroxidase, which catalyzes the enantioselective oxidation of prochiral sulfides with h(2)o(2) affording the s-sulfoxide, e.g. in 66% ee at 100% conversion for ...	2000	10581439
the involvement of helix pomatia lectin (hpa) binding n-acetylgalactosamine glycans in cancer progression.	the lectin from helix pomatia, the roman snail (hpa), recognises terminal alpha n-acetylgalactosamine residues. a large number of lectin histochemical studies have demonstrated that expression of hpa-binding glycoproteins by cancer cells to be a marker of metastatic competence and poor prognosis in a range of common human adenocarcinomas, including those of breast, stomach, ovary, oesophagus, colorectum, thyroid and prostate. around 80% of metastases arising from primary breast cancer are predic ...	2000	10668205
effects of valproate derivatives i. antiepileptic efficacy of amides, structural analogs and esters.	derivatives of the antiepileptic drug valproate (vpa, 2-propylpentanoic acid) have been synthesized and tested in order to improve the intracellular availability of vpa. the buccal ganglia of helix pomatia were used as a test nervous system and antiepileptic efficacies were reconfirmed using rat cortex in vivo. epileptiform activities consisted of typical paroxysmal depolarization shifts (pds) which appeared in the identified neuron b3 with application of pentylenetetrazol. epileptiform activiti ...	2000	10670421
electrospray ionization mass spectrometry of zinc, cadmium, and copper metallothioneins: evidence for metal-binding cooperativity.	electrospray ionization (esi) mass spectra of both well-characterized and novel metallothioneins (mts) from various species were recorded to explore their metal-ion-binding modes and stoichiometries. the esi mass spectra of the zinc- and cadmium-binding mts showed a single main peak corresponding to metal-to-protein ratios of 4, 6, or 7. these findings combined with data obtained by other methods suggest that these mts bind zinc or cadmium in a single predominant form and are consistent with the ...	2000	10716192
lectin ligands on human dendritic cells and identification of a peanut agglutinin positive subset in blood.	as only a few cell surface markers for dendritic cells (dc) have been identified to date, this study examined the expression of ligands for lectin on different human dc populations. the ability of concanavalin a (con a), wheat germ agglutinin (wga), peanut agglutinin (pna), and helix pomatia (hpa) to bind to cell lines and pbmc and dc populations was analyzed by flow cytometry and specificity of binding confirmed using inhibitory and noninhibitory sugars. the cell lines showed non-lineage-restri ...	2000	10716881
effects of lectins on calcification by vesicles isolated from aortas of cholesterol-fed rabbits.	advanced vascular calcification in atherosclerosis weakens arterial walls, thereby imposing a serious rupturing effect. however, the mechanism of dystrophic calcification remains unknown. although accumulating morphological and biochemical evidence reveals a role for calcifiable vesicles in plaque calcification, the mechanism of vesicle-mediated calcification has not been fully explored. to study whether vesicles' membrane components, such as carbohydrates, may have a role in vesicle-mediated ca ...	2000	10727613
histological disorders related to the focal disappearance of the epiphyseal growth plate in rats induced by high dose of vitamin a.	the histological disorders related to the focal disappearance of the epiphyseal growth plate were examined histochemically in the proximal tibia of rats administered a high dose of vitamin a. animals were given 100,000 iu/100 g body weight/day of vitamin a for 5 days from 4 weeks after birth (va rats) or given deionized water as control and sacrificed on day 12 and 19 of the experiment. tibiae were examined by immunohistochemistry for type i, ii and x collagens, lectin-histochemistry for helix p ...	2000	10770602
characterisation of lectin binding patterns of mouse bronchiolar and rat alveolar epithelial cells in culture.	lung epithelial cell differentiation pathways remain unclear. this is due in part to the plasticity of these cells and the lack of markers which accurately reflect their differentiation status. the aim of this study was to determine if lectin binding properties are useful determinants of functional differentiation status in vitro. mouse clara cells were cultured for 5 days. during this time, no alteration in differentiation was evident by electron microscopy. no significant alteration in binding ...	2000	10805383
stability and enzymatic hydrolysis of quaternary ammonium-linked glucuronide metabolites of drugs with an aliphatic tertiary amine-implications for analysis.	quaternary ammonium-linked glucuronide (n+-glucuronide) metabolites formed at aliphatic tertiary amine functional groups of xenobiotics have not been previously systematically studied with respect to their stability over a wide ph range and the ease of enzymatic hydrolysis by beta-glucuronidase from various sources. three and four n+-glucuronide metabolites were respectively studied regarding their non-enzymatic and enzymatic stabilities where the metabolites were quantified by hplc procedures. ...	2000	10815723
development of conjunctival goblet cells and their neuroreceptor subtype expression.	to investigate expression of muscarinic, cholinergic, and adrenergic receptors on developing conjunctival goblet cells.	2000	10892854
time-dependent actions of aluminates on membrane and action potentials of snail neurons.	aluminum (al) is one of the elements, which is frequently subjected to experiments, however, the neurological observations with it are rather conflicting. the cause of this controversiality is not known but relates to some human disorders such as alzheimer's disease and others as well. we studied the time-dependent actions of alcl3 base solutions on resting membrane potential (em), input resistance (rin) and spike shape in giant neurons of the snail helix pomatia l. at ph 7.7 and room temperatur ...	2000	11034155
cloning and sequencing of cdnas encoding for a novel copper-specific metallothionein and two cadmium-inducible metallothioneins from the blue crab callinectes sapidus.	metallothioneins (mts) are cysteine-rich metal-binding proteins found in micro-organisms, plants and all invertebrate and vertebrate animals. unicellular eukaryotes such as yeast have a copper-mt whose synthesis is induced by a copper-activated transcription factor. most higher organisms have two major cadmium/zinc mt isoforms, whose synthesis is controlled by a zinc-activated transcription factor. the blue crab, callinectes sapidus, has two cadmium-inducible isoforms, cdmt-i and cdmt-ii, and a ...	2000	11790353
abalone (haliotis tuberculata) hemocyanin type 1 (hth1). organization of the approximately 400 kda subunit, and amino acid sequence of its functional units f, g and h.	we have identified two separate hemocyanin types (hth1 and hth2) in the european abalone haliotis tuberculata. hth1/hth2 hybrid molecules were not found. by selective dissociation of hth2 we isolated hth1 which, as revealed by electron microscopy and sds/page, is present as didecamers of a approximately 400 kda subunit. immunologically, hth1 and hth2 correspond to keyhole limpet hemocyanin (klh)1 and klh2, respectively, the two well-studied hemocyanin types of the closely related marine gastropo ...	1999	10447670
[caragana arborescens lectin--a marker of myoepitheliocytes of salivary glands].	submaxillary salivary gland preparations of a horse, a cow, a pig, a rabbit and white outbred rats were stained with peroxidase-labeled lectins of different carbohydrate specificity. it was found that n-acetyl-d-galactosamine-specific lectins (caragana arborescens lam., phaseolus unatus seeds, helix pomatia sails) bind selectively with myoepitheliocytes. best marker of the latter proved to be caragana arborescens lectin, consisting of two components which differ in their affinity to n-acetyl-d-g ...	1999	10486811
lectins in drug delivery: a study of the acute local irritancy of the lectins from solanum tuberosum and helix pomatia.	lectins are proteins or glycoproteins of non-immune origin capable of binding to one or more specific sugar residues. the potential for using lectins as a means of 'anchoring' a drug delivery system to the mucosal surfaces of the eye has been investigated in previous work, with the lectins from solanum tuberosum and helix pomatia showing particular promise. in this study the acute local dermal irritancy of these lectins, in terms of their potential to cause inflammation and tissue necrosis, was ...	1999	10494002
determination of urinary 18 beta-glycyrrhetinic acid by gas chromatography and its clinical application in man.	a sensitive and quantitative gas chromatographic assay for the determination of 18 beta-glycyrrhetinic acid (18 beta-ga), the main metabolite of glycyrrhizin after oral licorice consumption in human urine, has been developed and validated. for the extraction of 18 beta-ga from urine two sep-pak c18 extractions, hydrolysis with helix pomatia and three liquid-liquid extractions were performed, using 18 alpha-glycyrrhetinic acid (18 alpha-ga) as internal standard. both 18 beta-ga and internal stand ...	1999	10510787
development of a simple method for the determination of genistein, daidzein, biochanin a, and formononetin (biochanin b) in human urine.	a simple method was developed for the determination of free and/or total isoflavones daidzein, genistein, and their respective 4'-methoxy derivatives biochanin a and formononetin (biochanin b) at low levels in human urine. a solid-phase extraction on octadecyl silica (c(18)) columns was used for the isolation of the phytoestrogens from the matrix. an extraction on a chemelut 1010 column connected on-line to a florisil cartridge by a teflon stopcock was used for effective eluate purification. a m ...	1999	10552674
muc5ac mucin is a component of the human precorneal tear film.	mucins are important structural and functional components of the precorneal tear film, yet little is known of their composition and synthesis. the mrnas of muc1, muc4, and muc5ac have previously been identified in human conjunctiva. of these, only muc5ac mrna appears to be associated with goblet cells. the purpose of the this study was to quantify muc5ac transcript levels, to identify muc5ac protein in conjunctiva, tears, and goblet cells and to determine whether this mucin is secreted in respon ...	1999	9888425
innate immunity in insects: the role of multiple, endogenous serum lectins in the recognition of foreign invaders in the cockroach, blaberus discoidalis.	unlike vertebrates, insects do not have an ab-based nonself recognition system, and must rely totally on innate immunity to defend themselves from microbial invaders. the most likely candidates for recognizing foreign material in insects are the lectins, which have already been shown to be important in mammalian innate immunity. the hemolymph of the cockroach, blaberus discoidalis, contains multiple lectins, designated bdl1, bdl2, bdl3, and gsl (beta-1,3-glucan-specific lectin), two of which, na ...	1999	9973417
the use of the lectin helix pomatia agglutinin (hpa) as a prognostic indicator and as a tool in cancer research.	progress in treatment for cancer has enabled extension of the disease-free interval, and of the quality of life for patients, but there has been very little improvement in overall survival rates. the main reason for this has been the ineffectiveness of current therapies to kill all the cancer cells once they have spread to distant sites to form metastatic deposits. one marker which has proved to be useful in identifying those cancers which have the potential to spread is the lectin helix pomatia ...	1999	9987666
a two-component affinity chromatography purification of helix pomatia arylsulfatase by tyrosine vanadate.	the inhibition of helix pomatia arylsulfatase by the synergistic combination of n-acetyl-l-tyrosine ethyl ester and vanadate has been extended to affinity chromatography for purification. in the presence of vanadate, l-tyrosine ethyl ester (tee), immobilized on ch-sepharose 4b retained arylsulfatase from the digestive juice or lyophilized powder of h. pomatia. no enzyme was retained without vanadate or with arsenate or phosphate. arylsulfatase was eluted from the column matrix by removing the va ...	1999	10049673
glycoconjugate expression in follicle-associated epithelium (fae) covering the nasal-associated lymphoid tissue (nalt) in specific pathogen-free and conventional rats.	we examined lectin-histochemically the glycoconjugate expression in the follicle-associated epithelium (fae) covering the nasal-associated lymphoid tissue (nalt) in the rat under specific pathogen-free (spf) and conventional (cv) conditions and compared the results for spf and cv rats as well as for membranous (m) cells and adjacent ciliated respiratory epithelial (cre) cells in fae. n-acetylgalactosamine-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), glycine max (sba) and vicia ...	1999	10067202
[the bronchorelaxant effect of helicidine, a helix pomatia extract, interferes with prostaglandin e2].	helicidine is a biological extract prepared from the snail helix pomatia l. and used in man as an anti-tussive agent. however, its mechanisms of action are not fully defined. in this study, we have investigated a possible relaxant effect of helicidine on guinea-pig airway smooth muscle and evaluated the role of prostanoids and airway epithelium in this relaxation. h. pomatia extract (0.001-1 mg/ml) induced a dose-dependent relaxation of guinea-pig trachea pre-contracted with histamine both in th ...	1999	10081783
s100 calcium binding protein affects neuronal electrical discharge activity by modulation of potassium currents.	s100 calcium binding protein has been associated with a variety of intra- and extracellular calcium-mediated functions, including learning and memory. we have previously localized s100-immunoreactive neurons correlated with spontaneous discharge activity in the central nervous system of the mollusc, helix pomatia. in this study, we further investigated the effects of s100 (s100b and s100a1) on electrical discharge activity and membrane currents of helix neurons, using current- and voltage-clamp ...	1999	10215154
[use of estrone sulfate determination in goat blood for the detection of pregnancy and prediction of fetal number].	the study was carried out on 50 goats of polish white breed. blood samples were taken on day 60 after mating from v. jugularis. pregnancy diagnosis was made using transcutan ultrasound scanning (pie medical scanner 2000 with a 5 mhz curved array probe). the estrone sulphate level was determined by ria method after extraction and hydrolysis with arylsulphatase/glucuronidase from helix pomatia. as a threshold value for differentiation of pregnant from nonpregnant goats 1 ng/ml was assumed. in eigh ...	1999	10326236
intracellular transport and maturation pathway of human herpesvirus 6.	a peculiar characteristic of cells infected with human herpesvirus 6 (hhv6) is the absence of viral glycoproteins on the plasma membrane, which may reflect an atypical intracellular transport of the virions and/or the viral glycoproteins, different from that of the other members of the herpesvirus family. to investigate the maturation pathway of hhv-6 in the human t lymphoid cell line hsb-2, we used lectin cytochemistry and immunogold labeling combined with several electron microscopical techniq ...	1999	10329556
lectin binding sites in parotid acinar secretory granules of normal and isoproterenol treated rat.	lectin staining patterns in secretory granules of rat parotid gland acinar cell of untreated and isoproterenol-injected animals were examined by electron microscopy. we used four lectin-gold complexes: ulex europaeus agglutinin i (uea-i), helix pomatia agglutinin (hpa), wheat germ agglutinin (wga), glycine max agglutinin (sba). specimens were low temperature embedded in the hydrophilic lowicryl k4m resin. the normal acinar cells produced glycoconjugates which were positive for all of the lectins ...	1999	10363359
beta2-agonist abuse in food producing animals: use of in vitro liver preparations to assess biotransformation and potential target residues for surveillance.	1. the biotransformation of [3h]clenbuterol, [3h]salbutamol, [14c]salmeterol and 7-ethoxycoumarin by bovine liver was investigated by incubation with freshly prepared microsomes, suspension and monolayer cultures of isolated hepatocytes, precision-cut (250 microm) and chopped (600 microm) tissue slices. 2. radio-hplc analysis indicated that the saligenin beta2-agonists salmeterol and salbutamol were extensively metabolized by all intact cell preparations. a single major product (smm1) was eviden ...	1999	10379986
enzyme immunoassay for the measurement of 17alpha-estradiol 17-n-acetylglucosaminide in rabbit urine.	17alpha-estradiol 17-n-acetylglucosaminide (17alphae2 17nag) is an estrogen metabolite hitherto obtained only in rabbits. to gain insight into this unique conjugate, an enzyme immunoassay (eia) was established by using antiserum elicited against 3-[3-(1-carboxypropyl)] ether of 17alphae2 17nag-bovine serum albumin conjugate; horseradish peroxidase, as a label; and 3,3',5,5'-tetramethylbenzidine, as a chromogen. the method proved to be specific, and the detection range of the assay was 0.20-10.00 ...	1999	10399888
subsets of malignant lymphomas in children related to the cell phenotype.	we studied the lymphomatous cells of 39 children presenting with the classical features of malignant lymphoma. twenty-two had t lymphoblasts. we could classify these patients into three subsets: the t lymphoblasts from children group 1 displayed antigen(s) shared by a thymocyte subpopulation, had terminal deoxynucleotidyl transferase (tdt), but no affinity for peanut agglutinin (pna). the t lymphoblasts from children group 2 lacked the thymocyte antigen(s), had no tdt, but showed affinity for pn ...	1999	91395
[structuro-functional analysis of identified grape snail neurons].	the study of microanatomy of central neurons in helix pomatia showed that each identified neurone has a constant number of definitely directed processes. the branching pattern of the processes is also specific for each neurone, but in the same cell the number of the branches may vary. it is concluded, that the general morphology of the neurone is its sufficiently stable characteristic and may serve as a criterion for its identification. the possible functional role of different types of the neur ...	1999	93832
effects of calcium and calcium-chelating agents on the inward and outward current in the membrane of mollusc neurones.	1. effects of internal and external ca and ca-chelating agents, egta and edta on transmembrane ionic currents were studied in isolated, internally dialysed neurones from the molluscs, helix pomatia and limnea stagnalis.2. the possible pharmacological effect of internally applied egta was investigated on the background of constant free ca concentration (5.3 x 10(-9)m). egta had no effect on ca and na inward currents but considerably depressed the delayed k outward current. no effective removal of ...	1999	409839
polycystic disease of the kidney. evaluation and classification based on nephron segment and cell-type specific markers.	the usefulness of various segment and cell-type specific antibody, lectin and functional markers in the study of cystic renal lesions was evaluated. for this purpose, kidneys from recessive polycystic kidney disease (rpkd), thought to involve mainly the collecting ducts, and cystic kidneys of meckel's syndrome (ms), which show dilation randomly along the nephron, were studied. the segment (and differentiation-stage)-specific anti-brush-border (specific for proximal tubules) antibodies stained mo ...	1999	1690315
[effect of schizophrenic patients' serum immunoglobulins on nerve cell potentials].	with the aid of the microelectrode method, the influence of different protein fractions isolated from the blood serum of schizophrenic patients and normals on an edible snail was studied. the fractions were isolated by means of ion exchange chromatography and contained immunoglobulins (igg, iga, igm). it was established that the fraction of the blood serum in schizophrenic patients, containing igm is significantly stronger than the respective fraction in normals, depolarizing and inactivating th ...	1998	106612
changes in localization of chosen lectins in gerbil's brain submitted to 3 and 4 minute-long cns ischemia.	the gerbils brains after 3- and 4-minute-long ischemia caused by bilateral common carotid artery occlusion and 14 days survival were investigated using lectin techniques. chosen lectins, represented by synthetic plant glycoproteids, which are specifically bound to particular sugar residues (receptors) located on the cell surfaces were examined. lectins recognizing the following sugar residues have been used in our experiment: 1. n-acetyl-d-galactosyl (using helix pomatia agg., hpa) 2. alpha-d-ma ...	1998	9595860
determination of oestrogen concentrations in bovine plasma by a recombinant oestrogen receptor-reporter gene yeast bioassay.	a recombinant cell yeast bioassay (rcba) was applied to the generic measurement of bovine plasma oestrogen concentration. samples were prepared by diethyl ether extraction of plasma following addition of [3h]17 beta-oestradiol as internal standard; organic and aqueous phases were separated by freezing (recovery 97.1 +/- 0.7%) and dried extract reconstituted in culture medium (recovery 31.4 +/- 4.5%). plasma oestrogen concentrations were measured by incubation of extracts with yeast containing a ...	1998	10435304
utility of isolated hepatocytes and radio-hplc-msn for the analysis of the metabolic fate of 19-nortestosterone laurate in cattle.	the metabolic fate of 19-nortestosterone laurate in cattle was investigated to evaluate target analyte(s) appropriate to surveillance for illicit use as a growth promoting agent. bovine hepatocytes were incubated with either [3h]19-nortestosterone laurate (19-ntl; 4-estren-17 beta-laurate-3-one) or [3h]19-nortestosterone (19-nt; 4-estren-17 beta-ol-3-one; nandrolone). hepatocyte medium was extracted with solid phase c18 media and analysed by narrow bore radio-hplc-msn (lcq, finnigan) to evaluate ...	1998	10435305
interference of helix pomatia extracts on the determination of methandriol in veterinary residue control analysis.	aqueous solutions containing methandriol (mad) were incubated with succus helix pomatia (shp) or beta-glucuronidase from escherichia coli (ec). shp, used for enzymatic hydrolysis of urinary steroid conjugates for residue analysis of anabolic agents, caused transformation of mad into methyltestosterone. no conversion occurred when bacterial beta-glucuronidase from e. coli was used.	1998	10435316
invasion potential and n-acetylgalactosamine expression in a human melanoma model.	reactivity of the n-acetylgalactosamine-binding helix pomatia agglutinin (hpa) in tumours has been associated with poor prognosis and metastasis development. in our lox/femx-i human melanoma model, the binding of hpa correlates with experimental lung metastasis formation in athymic nude mice. in the present study, the metastatic potential of 2 human melanoma cell lines (lox and femx-i) was assessed in relation to carbohydrate and invasive phenotype. immunocytological and invasion assays highligh ...	1998	9466664
steroid sulphatase: expression, isolation and inhibition for active-site identification studies.	steroid sulphatase, which can hydrolyse 3-hydroxysteroid sulphates, has important roles in several physiological and pathological processes. a number of steroid sulphatase inhibitors have now been developed, of which the most potent to date is oestrone-3-o-sulphamate (emate). this inhibitor inactivates steroid sulphatase in an irreversible, time- and concentration-dependent manner. in order to be able to use a radiolabelled derivative of emate to study the active site, it will be essential to pr ...	1998	9566745
core alpha1,3-fucose is a key part of the epitope recognized by antibodies reacting against plant n-linked oligosaccharides and is present in a wide variety of plant extracts.	carbohydrates have been suggested to account for some ige cross-reactions between various plant, insect, and mollusk extracts, while some igg antibodies have been successfully raised against plant glycoproteins. a rat monoclonal antibody raised against elderberry abscission tissue (yz1/2.23) and rabbit polyclonal antiserum against horseradish peroxidase were screened for reactivity in enzyme-linked immunosorbent assay against a range of plant glycoproteins and extracts as well as neoglycoprotein ...	1998	9621106
scavestrogen sulfamates: correlation between estrone sulfatase inhibiting and antioxidant effects.	in the present study estrone sulfatase (steryl-sulfatase; ec 3.1.6.2) and phenylsulfatase (arylsulfatase b; ec 3.1.6.1) inhibiting as well as antioxidant effects exerted by ring b,c unsaturated sulfamates of estrone (j 1025), 17 beta-estradiol (j 1054, j 1059, j 1067), and 17 alpha-estradiol (j 1051, j 1064, j 1065) were examined as compared with their parent compounds, j 994, j 995, and j 1050, using six different in vitro models: (i) estrone sulfatase activity in human placental microsomes, (i ...	1998	9635147
screening and confirmatory determination of ractopamine residues in calves treated with growth promoting doses of the beta-agonist.	ractopamine (rct) is a phenethanolamine member of the family of beta-adrenergic agonists (beta-agonists). this class of compounds have become notable for their properties of enhancing the growth rates of farm animal species but are not licensed for use in europe. an elisa procedure employing a polyclonal antibody raised in a goat was developed to detect rct residues in bovine urine samples. the assay had a high sensitivity (calibration curve mid-point of 22 pg per well), allowing the analysis of ...	1998	9709494
assembly and architecture of invertebrate cytoplasmic intermediate filaments reconcile features of vertebrate cytoplasmic and nuclear lamin-type intermediate filaments.	the two major intermediate filament (if) proteins from the esophagus epithelium of the snail helix pomatia and the two major if proteins from muscle tissue of the nematode ascaris suum were investigated under a variety of assembly conditions. the lowest-order complexes from each of the four protostomic invertebrate (p-inv) if proteins are parallel, unstaggered dimers involving two-stranded alpha-helical coiled coil formation of their approximately 350 amino acid residue central rod domain (i.e. ...	1998	9737925
protostrongylid nematode infection of chamois (rupicapra rupicapra) at the bauges massif (french alps).	from 1979 to 1985, protostrongylid infections (small lungworms) of chamois were studied at the bauges hunting reserve, in the western alps. fifty-two chamois were necropsied on 24 sites and lung nematodes identified to species. from june to november 1985, a more detailed study was undertaken on four sites where chamois were the only ruminants. first-stage larvae excreted in feces and infection of snails intermediate hosts were monitored. the protostrongylid species were, arranged in decreasing o ...	1998	9746285
regulation of ocular mucin secretion by p2y2 nucleotide receptors in rabbit and human conjunctiva.	the effects of adenine analogues on secretion of high molecular weight, mucin-like glycoproteins (mucins) by conjunctival goblet cells were investigated using isolated rabbit and human conjunctiva. mucin secretion was assayed using a quantitative dot-blot assay of helix pomatia agglutinin-horseradish peroxidase binding to mucins absorbed to nitrocellulose filters. in rabbit conjunctiva, exogenous atp (10(-7)-10(-3) m) induced a concentration-dependent, four-fold increase in mucin secretion that ...	1998	9778415
detection of inflammation- and neoplasia-associated alterations in human large intestine using plant/invertebrate lectins, galectin-1 and neoglycoproteins.	commonly, plant and invertebrate lectins are accepted glycohistochemical tools for the analysis of normal and altered structures of glycans in histology and pathology. mammalian lectins and neoglycoproteins are recent additions to this panel for the detection of lectin-reactive carbohydrate epitopes and glycoligand-binding sites. the binding profiles of these three types of probes were comparatively analyzed in normal, inflamed and neoplastic large intestine. in normal colonic mucosa the intrace ...	1998	9780361
viral glycoproteins accumulate in newly formed annulate lamellae following infection of lymphoid cells by human herpesvirus 6.	ultrastructural analysis of hsb-2 t-lymphoid cells and human cord blood mononuclear cells infected with human herpesvirus 6 revealed the presence, in the cell cytoplasm, of annulate lamellae (al), which were absent in uninfected cells. time course analysis of the appearance of al following viral infection showed that no al were visible within the first 72 h postinfection and that their formation correlated with the expression of the late viral glycoprotein gp116. the requirement of active viral ...	1998	9811708
lectin histochemistry of dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats.	a lectin histochemical study was carried out on the dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats (htrs) and wistar rats (wrs) at 3, 7 and 24 weeks of age to clarify the lectinhistochemical characteristics of the skin during their development. the lectins examined were concanavalia ensiformis (con a), dolichos biflorus agglutinin (dba), griffonia simpliciolia (gs-i), helix pomatia agglutinin (hpa), arachis hypogaea (pna), glycine maximus agglutinin (sba), ulex europeus agglutinin ( ...	1998	9816494
novel forms of protein glycosylation.	a large number of new glycans, derived from glycoproteins, has been characterized in the past few years. o-linked fucose was found in epidermal growth factor-like domains of several proteins. for the n-linked glycans of helix pomatia hemocyanin, novel types of antennae were identified. the positions of noncarbohydrate substituents were established in n-glycans. c-mannosylation of a tryptophan residue was discovered in human ribonuclease 2 and is the first example of c-glycosylation in glycoprote ...	1998	9818259
altered anionic gbm components in monoclonal antibody against slit diaphragm-injected proteinuric rats.	we previously reported that monoclonal antibody (mab) 5-1-6 bound to renal filtration slits induces massive proteinuria without causing ultrastructural changes in the glomerulus. this study evaluated the underlying mechanisms of the increase in glomerular permeability.	1998	9844125
comparison of four immunoassays for the detection of lorazepam in urine.	four healthy patient subjects were each given a single, 1-mg lorazepam tablet. urine samples from all patient subjects were collected at 12 intervals (0-2, 2-5, 5-8, 8-11, 11-14, 14-24, 24-26, 26-29, 29-32, 32-35, 35-38, and 38-48 hours). an aliquot from each urine collection was screened using cloned enzyme donor immunoassay (cedia), enzyme-multiplied immunoassay technique (emit) ii, emit dau, and fluorescence polarization immunoassay (fpia) without and with hydrolysis using beta-glucuronidase. ...	1998	9853986
comparative lectin-histochemistry on the pre-epithelial mucus layer in the distal colon of conventional and germ-free rats.	the mucin composition of the rat distal colonic pre-epithelial mucus layer (pml) was studied by lectin histochemistry in conventional (cv), and germ-free (gf) rats to define effects exerted by the gut flora. no peanut agglutinin (pna) binding was observed in the pml of gf rats, while the pml of their cv counterparts showed a considerable pna linkage, indicating terminal gal-beta1,3-galnac residues. soybean agglutinin (sba) and helix pomatia agglutinin (hpa) stained the pml mucins in cv and in gf ...	1998	11253810
developmental appearance and distribution of bone sialoprotein and osteopontin in human and rat cementum.	bone sialoprotein (bsp) and osteopontin (opn), two major noncollagenous proteins (ncps) in collagen-based mineralized tissues, have been implicated in mineral deposition and cell- and matrix-matrix interactions during root development. however, their role in cementogenesis is still a subject of debate. since distribution of proteins is indicative of function, we have analyzed their temporo-spatial appearance in relation to that of cementum collagen.	1998	9458064
do hpa and pha-l have the same binding pattern in metastasizing human breast and colon cancers?	glycoconjugates on the tumour cell surface are functionally important for the interaction of the tumour cell with its environment. several studies have demonstrated that particular carbohydrate residues on primary cancers are associated with metastasis. identification of such residues is possible using lectins, including helix pomatia agglutinin (hpa) which has a nominal monosaccharide specificity for n-acetyl galactosamine (galnac) and phaseolus vulgaris leucoagglutinin (pha-l) which recognizes ...	1998	9461027
lectin histochemical studies of mouse granulated metrial gland cells.	a lectin histochemical study has been carried out on mouse granulated metrial gland cells, the major leucocyte population that differentiates in the uterine wall in pregnancy. the binding characteristics of 26 lectins were examined using light microscopical methods. fourteen of the lectins, with affinities ranging through n-acetylgalactosamine, galactose, n-acetylglucosamine, mannose and sialic acid residues, bound to the cytoplasmic granules of granulated metrial gland cells, and each appeared ...	1997	9466156
experimental evidence of secretion by mouse granulated metrial gland cells.	granulated metrial gland cells are cytotoxic cells found in the uterine wall in pregnancy. in this study, the involvement of the cytoplasmic granules of mouse granulated metrial gland cells during cytolysis of fibrosarcoma wehi 164 cells was investigated in vitro. using the lectin helix pomatia agglutinin which binds to the granule membrane, but not to the cell membrane, of mouse granulated metrial gland cells, the limiting membrane of one or more cytoplasmic granules became incorporated into th ...	1997	9250711
galactose-deficient iga1 in sera of iga nephropathy patients is present in complexes with igg.	iga1 proteins from sera of patients with iga nephropathy (igan) are galactosylated to a lesser degree than those from healthy controls. the increased reactivity of intact or de-sialylated serum iga1 with n-acetylgalactosamine (galnac)-specific lectins, helix aspersa (haa) and caragana arborescens (caa) and de-sialylated iga1 with helix pomatia (hpa) and bauhinia purpurea (bpa) indicated that the gal deficiency is in glycans located in the hinge region of iga1 molecules. de-sialylated iga from se ...	1997	9264010
comparative abilities and optimal conditions for beta-glycosidase enzymes to hydrolyse the glucuronide, glucoside, and n-acetylglucosaminide conjugates of bile acids.	enzymatic hydrolyses were described for three variants of glycosidic conjugated bile acids with one beta-glucuronidase (helix pomatia), three beta-glucosidase (almonds, sweet almonds, and escherichia coli), and four beta-n-acetylglucosaminidase (jack beans, bovine kidney, human placenta, and diplococcus pneumoniae) preparations. the substrates include the beta-glucuronide, beta-glucoside, and beta-n-acetylglucosaminide conjugates of bile acids related to hyodeoxycholic, murideoxycholic, chenodeo ...	1997	9300125
differential binding properties of gal/galnac specific lectins available for characterization of glycoreceptors.	differentiating the binding properties of applied lectins should facilitate the selection of lectins for characterization of glycoreceptors on the cell surface. based on the binding specificities studied by inhibition assays of lectin-glycan interactions, over twenty gal and/or galnac specific lectins have been divided into eight groups according to their specificity for structural units (lectin determinants), which are the disaccharide as all or part of the determinants and of galnac alpha 1--> ...	1997	9343930
localization of procollagen i in the lysosome/endosome system of human fibroblasts.	a significant amount of newly synthesized collagen is degraded intracellularly rather than secreted, but there is controversy about whether this process occurs in the lysosomes. we addressed this problem using confocal microscopy and immunofluorescence imaging to study the distribution of procollagen i in the golgi and the lysosome/endosome system of cultured human fibroblasts. cells were incubated under basal conditions and then permeabilized and exposed to fluorescently tagged probes for proco ...	1997	9344594
primary structure of 21 novel monoantennary and diantennary n-linked carbohydrate chains from alphad-hemocyanin of helix pomatia.	the primary structures of 21 novel monoantennary and diantennary n-glycans of the glycoprotein alphad-hemocyanin (alphad-hc) of helix pomatia have been determined. outer oligosaccharide fragments (antennae) were released from the glycoprotein by smith degradation of an alphad-hc pronase digest. the major antenna, obtained following hplc fractionation on lichrosorb-nh2, was characterized using 1h-nmr spectroscopy, fast-atom-bombardment mass spectrometry, and linkage analysis, and corresponds to a ...	1997	9363772
lectins and antibodies to blood group antigens as markers for the basal cells of the human respiratory epithelium.	we used a pattern of 30 lectins and antibodies against antigens of the abo-blood group system to find specific and sensitive markers for the basal cells of the human respiratory surface epithelium. three lectins always stained the basal cells: aaptos papillata agglutinin i (apa i), peanut agglutinin (pna), and wheat germ agglutinin (wga): other lectins and the antibodies gave positive results only in tissue of secretors (blood group antigens in secretions) and these were dependent on the abo-blo ...	1997	9376654
lectin histochemical hpa-binding pattern of human breast and colon cancers is associated with metastases formation in severe combined immunodeficient mice.	metastasis formation is a major clinical problem in cancer treatment, and no significant progress in the treatment of metastatic spread has been made. this apparent lack of progress is partly caused by the absence of clinically relevant animal models of metastases. the binding of the lectin helix pomatia agglutinin (hpa) has been associated with a poor prognosis in breast and colon cancer patients. hpa-positive and -negative human breast and colon cancer cell lines were transplanted into severe ...	1997	9413741
lectin binding sites on cd34+ human haematopoietic stem cells and lymphocytes from peripheral blood: an ultrastructural post-embedding study.	this study was performed to obtain a better insight into the glycosylation pattern of human cd34+ haematopoietic stem cells and lymphocytes from peripheral blood using an ultrastructural post-embedding technique. lectins applied were derived from canavalia ensiformis (con a), triticum vulgare (wga), lycopersicon esculentum (lea), limulus polyphemus (lpa), ulex europaeus-i (uea-i), bauhinia purpurea (bpa), glycine max (sba), helix pomatia (hpa), arachis hypogaea (pna) and erythrina cristagalli (e ...	1997	9413743
lectin histochemistry of the rat brain following thioacetamide-induced hepatic failure.	biotinyl derivatives of several lectins were used to study the localization of glycoconjugates in the cerebral microcapillaries and various brains of rats given at 24-h intervals two i.p. administrations of a hepatotoxin-thioacetamide (taa) and examined 21 d posttreatment. at this time, the rats were asymptomatic with regard to hepatic encephalopathy but showed specific and selective changes in the blood-brain-barrier (bbb) transport of basic amino acid, but no bbb damage, and region-specific ne ...	1997	9437665
evidence for a cysteine-histidine thioether bridge in functional units of molluscan haemocyanins and location of the disulfide bridges in functional units d and g of the betac-haemocyanin of helix pomatia.	in functional units d and g from the betac-haemocyanin of the gastropod helix pomatia, aminoacid analysis in the presence of dimethyl sulfoxide showed the occurrence of seven cysteine residues. titration with 5,5'-dithiobis(2-nitrobenzoic acid), however, did not reveal any free thiol group. pepsinolysis at ph 2.0 followed by amino acid analysis and partial sequencing of the cysteine-containing peptides showed that six cysteine residues are involved in the formation of three disulfide bridges at ...	1997	9342242
[the monoamines in molluscs. ii. dopamine and neurotransmission. cardiac dopaminergic innervation in helix pomatia (author's transl)].	in the molluscs, dopamine is very probably a chemical transmitter at the level of both the central nervous system and certain peripheral structures. the heart of helix pomatia does not have any intrinsic innervation, but it receives extrinsic innervation from fibres coming from the visceral nerve. formaldehyde fluorescence histochemistry localizes the cardiac catecholamines in some of these fibres and in their endings. however, dopamine, which dominates, does not seem to be a transmitter involve ...	1997	232723
metallothionein research in terrestrial invertebrates: synopsis and perspectives.	while most of metallothionein research during the past years has been carried out on mammals or vertebrates, only relatively few studies have been directed towards invertebrates. even fewer investigations have focussed on terrestrial invertebrates. the best studied metallothioneins and/or metallothionein genes among terrestrial invertebrates are those from an insect species (drosophila melanogaster), a nematode (caenorhabditis elegans) and some terrestrial gastropods (helix pomatia, arianta arbu ...	1996	8646613
changes in keratinocyte differentiation during accelerated repopulation of the irradiated mouse epidermis.	epidermal stem cells accelerate their repopulation rate during fractionated irradiation. to study the changes in keratinocyte differentiation associated with radiation-induced repopulation, we investigated the expression of a panel of 14 monoclonal or polyclonal antibodies against keratins, involucrin and others, as well as the expression of 15 lectins in the irradiated mouse leg skin. tissue samples were collected after 1, 2, and 3 weeks of daily irradiation with 3 gy per fraction. abnormal mor ...	1996	8691028
characterization of the mucins produced by normal human colonocytes in primary culture.	mature goblet cells filled with mucin ready for secretion represent about one third of the cells in primary cultures of human colonocytes. in the present study characterization of the mucins produced by cultured human colonocytes was made by histochemical methods by lectin and monoclonal antibody binding. two monoclonal antibodies and three lectins (dolichos biflorus (dba), helix pomatia (hpa) and arachis hypogea (pna) recognizing epitopes or sugar haptens characteristic of different stages of m ...	1996	8739831
the expulsion of echinostoma trivolvis from c3h mice: differences in glycoconjugates in mouse versus hamster small intestinal mucosa during infection.	mucosal glycoconjugates were examined in c3h mice and in hamster small intestines infected with echinostoma trivolvis and in uninfected rodents, using periodic-acid schiff (pas) and high-iron diamine-alcian blue (hid-ab) staining and three different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), helix pomatia agglutinin (hpa) and griffonia simplicifolia agglutinin (gsa-ii). lectin-labelling by electron microscopy was also undertaken with wga and hpa lectin-gold probes. hid-a ...	1996	8960206
does the lectin helix pomatia agglutinin bind to hyaluronic acid in breast and colon cancer?	the lectin helix pomatia agglutinin (hpa) has been successfully used as an indicator of metastatic spread in a number of epithelial neoplasms including breast, colorectal, gastric, prostate and oesophageal cancers. despite its use, the binding partners of hpa in tissue sections have not been defined at the molecular level. hpa has two main binding specificities: 1) n-acetylgalactosamine (galnac) and 2) n-acetylglucosamine (glucnac). in order to determine whether hpa binds to hyaluronic acid (a g ...	1996	8960307
transplantation of a human ovarian cystadenocarcinoma into severe combined immunodeficient (scid) mice--formation of metastases without significant alteration of the tumour cell phenotype.	human ovarian papillary cystadenocarcinoma cells were injected intraperitoneally into severe combined immunodeficient (scid) mice. after intraperitoneal application the cells, designated sotü, grew well in vivo, lodged on to the peritoneum, formed local metastatic deposits, led to the development of ascites in the mice and formed distant metastases in the lungs. if lodged in the ovary, the morphology of the sotü tumour remarkably resembled that of the primary tumour in the patient. in contrast, ...	1996	8977374