Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| detection of the dna of borrelia afzelii, anaplasma phagocytophilum and babesia canis in blood samples from dogs in warsaw. | each month, from march 2003 to february 2004, 34 blood samples from dogs were randomly selected from the blood samples delivered to two veterinary laboratories in warsaw and tested for the dna of borrelia burgdorferi sensu lato, anaplasma phagocytophilum, babesia canis and hepatozoon canis. borrelia dna was detected in seven of the 408 dogs, a phagocytophilum dna was found in two, and b canis dna was found in 48 (11.8 per cent). the dna of h canis was not found in any of the blood samples. seque ... | 2009 | 19363228 |
| use of chimeric dna-rna primers in quantitative pcr for detection of ehrlichia canis and babesia canis. | to overcome the problem of nonspecific by-products in quantitative pcr (qpcr) assays, we constructed dna-rna chimeric primers and evaluated their use in the detection and quantification of the ehrlichia canis 16s rrna, babesia canis hsp70, and canine beta-actin genes. several rna bases were incorporated into specific positions in the dna primers, while no rna stretches were allowed. qpcr reactions were carried out without preamplification steps. this resulted in decreased formation of undesirabl ... | 2009 | 19633128 |
| hemoprotozoa of domestic animals in france: prevalence and molecular characterization. | very limited information is available on epizootiology of haematozoan infections in french domestic animals. in an attempt to address this issue, prevalence of piroplasmida was studied in carnivores and ruminants, whereas prevalence of hepatozoon spp. was only investigated in carnivores. in total, 383 animals were included in the survey (namely 116 cats, 108 dogs, 91 sheep and 68 cows). parasite diagnosis was carried out using molecular methods such as pcr and sequencing of the 18s rrna gene. in ... | 2009 | 19013719 |
| pcr survey of vectorborne pathogens in dogs living in and around barcelona, an area endemic for leishmaniasis. | blood samples from 153 dogs living in and around barcelona were assayed for leishmania infantum and ehrlichia, anaplasma, rickettsia, bartonella, hepatozoon, babesia and theileria species by pcr amplification of dna, and the amplicons obtained were sequenced. the prevalence of the infectious agents was l infantum (29.4 per cent), ehrlichia and anaplasma species (4.0 per cent), hepatozoon canis (3.3 per cent), babesia canis vogeli (2.0 per cent), babesia gibsoni (2.0 per cent), babesia canis cani ... | 2009 | 19168881 |
| systemic inflammatory responses in dogs experimentally infected with babesia canis; a haematological study. | a detailed haematological study of dogs that were infected with low, moderate or high numbers of babesia canis-infected red blood cells was performed in an attempt to elucidate the pathogenesis early after b. canis infection. results showed that upon infection the c-reactive protein (crp) level in plasma increased prior to the detection of parasites in the blood indicative of an acute phase reaction. the response was further characterised by fever, fibrinogenaemia, thrombocytopenia and leucopoen ... | 2009 | 19269099 |
| comparison of babesia rossi and babesia canis isolates with emphasis on effects of vaccination with soluble parasite antigens: a review. | babesia canis and b. rossi are large babesia species that infect dogs and cause clinical disease. the spectrum of disease is highly diverse with either parasite, but upon evaluation of field cases it has been suggested that in general b. rossi is more virulent than b. canis. this difference was also found in experimental infections using b. canis and b. rossi isolates and appeared to be related to a difference in parasitaemia. whether this difference reflects the essential difference between b. ... | 2009 | 19831266 |
| detection of a large unnamed babesia piroplasm originally identified in dogs in north carolina in a dog with no history of travel to that state. | a 12-year-old 46-kg (101.2-lb) sexually intact male labrador retriever was evaluated because of lymphadenomegaly. the dog resided in texas, and its travel history included many southeastern and eastern shore states but not north carolina. | 2009 | 19793016 |
| alterations in some blood coagulation parameters in naturally occurring cases of canine babesiosis. | changes in coagulation parameters were studied in dogs naturally infected with babesia canis canis (n = 30), and haemostasis was evaluated and compared to values obtained from healthy dogs (n = 29). to date, there have not been any studies examining the dynamics of thrombin-antithrombin complex formation in cases of canine babesiosis. coagulation parameters evaluated before (day 0) and on days 1, 2, and 3 after treatment with imidocarb (6 mg/kg inj. s.c.) included the determination of platelet c ... | 2009 | 19584042 |
| canine piroplasmosis in italy: epidemiological aspects in vertebrate and invertebrate hosts. | in italy, canine piroplasmosis is believed to be widespread, but few data are available on its presence in most areas. in 2005 and 2006, vertebrate and invertebrate hosts were investigated in central and northern regions of the country. microscopy on blood smears, molecular tools and serological tests were applied to 420 blood samples collected from dogs, in order to evaluate the presence of these protozoa and to identify possible risk factors. moreover, ticks were analyzed by molecular techniqu ... | 2009 | 19632786 |
| diversity ofbabesia and theileria species in symptomatic and asymptomatic dogs in croatia. | babesiosis, the disease caused by tick-borne hematozoan parasites of the genus babesia, is particularly common in dogs, and is caused by several "large" species ofbabesia, as well as by an increasing number of "small" species of babesia, some of which appear to be more closely related to members of the genus theileria. in this work, blood samples were collected from 848 randomly selected, asymptomatic dogs and from 81 symptomatic dogs, microscopically positive for babesia, and characterized by p ... | 2009 | 19640468 |
| detection of erythrocyte binding igm and igg by flow cytometry in sick dogs with babesia canis canis or babesia canis vogeli infection. | the aim of this study was to examine by means of flow cytometry immunophenotyping (fci) if sick dogs infected with babesia canis canis (b. c. canis) or babesia canis vogeli (b. c. vogeli) had anti-erythrocyte membrane binding igg and/or igm at the time of diagnosis. diagnosis of babesia infection was assessed by blood smear and by pcr-restriction fragment length polymorphism analysis in 30 sick dogs. signalment, clinical history, physical examination and laboratory tests of b. c. canis (n=24) an ... | 2009 | 19269745 |
| molecular analysis of the rrna genes of babesia spp and ehrlichia canis detected in dogs from ribeirão preto, brazil. | the partial dna sequences of the 18s rrna gene of babesia canis and the 16s rrna gene of ehrlichia canis detected in dogs from ribeirão preto, brazil, were compared to sequences from other strains deposited in genbank. the e. canis strain circulating in ribeirão preto is identical to other strains previously detected in the region, whereas the subspecies babesia canis vogeli is the main babesia strain circulating in dogs from ribeirão preto. | 2009 | 24031351 |
| the clinical course of babesiosis in 76 dogs infected with protozoan parasites babesia canis canis. | the aim of the study was to trace the clinical course of babesiosis in 76 dogs infected with babesia canis protozoa and to assess the usefulness of pcr method in the routine diagnosis of the disease. the investigations were conducted in three successive seasons of the biological activity of ticks on dogs displaying possible clinical signs of babesiosis, the latter assigned individual numbers from 001 to 076. all the animals underwent routine clinical examinations and blood was collected for haem ... | 2009 | 19459444 |
| diversity of babesia and theileria species in symptomatic and asymptomatic dogs in croatia. | babesiosis, the disease caused by tick-borne hematozoan parasites of the genus babesia, is particularly common in dogs, and is caused by several "large" species of babesia, as well as by an increasing number of "small" species of babesia, some of which appear to be more closely related to members of the genus theileria. in this work, blood samples were collected from 848 randomly selected, asymptomatic dogs and from 81 symptomatic dogs, microscopically positive for babesia, and characterised by ... | 2009 | 19367832 |
| canine babesiosis caused by babesia canis vogeli in rural areas of the state of minas gerais, brazil and factors associated with its seroprevalence. | this epidemiological survey on canine babesiosis was carried out in three distinct rural regions (lavras, belo horizonte and nanuque) of the state of minas gerais, brazil. ticks and blood samples were collected during a dry season (lavras, n=92; belo horizonte, n=50; nanuque, n=102) and the subsequent rainy season (lavras, n=71; belo horizonte, n=28; nanuque, n=66) from dogs living on farms. plasma samples were analyzed by the indirect fluorescent antibody test for detection of anti-babesia cani ... | 2009 | 18723199 |
| new localities of dermacentor reticulatus tick (vector of babesia canis canis) in central and eastern poland. | dermacentor reticulatus tick is a vector and final host of babesia canis canis, protozoan parasite of the dog. in poland and other european countries, endemic regions for canine babesiosis caused by b. canis canis are the same as endemic regions for d. reticulatus. in many of these regions, canine babesiosis is the most prevalent tick-borne disease in dogs. in europe, increasing range of geographical distribution of d. reticulatus is observed. a consequence of this fact may be increasing range o ... | 2009 | 20169932 |
| [occurrence and molecular characterization of babesia species in a canine hospital population in the londrina region, parana state, brazil]. | canine babesiosis is a worldwide disease caused by the protozoan of babesia genus. babesia canis and b. gibsoni are both species that naturally infect dogs. the objective of this study was to evaluate the infection of babesia species in dogs attended at the londrina state university veterinary teaching hospital (hv-uel). it was selected 282 dogs seen at the londrina state university veterinary teaching hospital (hv-uel) between april of 2005 and may of 2006. they presented anemia (packed cell vo ... | 2008 | 20059862 |
| babesia canis canis and babesia canis vogeli clinicopathological findings and dna detection by means of pcr-rflp in blood from italian dogs suspected of tick-borne disease. | the aims of this study were to determine the presence of babesia spp. in blood samples from italian dogs with clinical signs compatible with tick-borne diseases by means of pcr-restriction fragment length polymorphism (rflp) and describe the clinicopathological findings of dogs with babesia infection. we evaluated the majority of canine babesiosis cases by means of clinical history, physical examination, hematological, biochemical, serum electrophoresis, urinalysis and hemostatic tests. forty-fi ... | 2008 | 18789581 |
| molecular characterization of babesia kiwiensis from the brown kiwi (apteryx mantelli). | to further investigate the recently described avian piroplasm, babesia kiwiensis, blood samples were collected from 13 wild-caught and 8 zoo-captive brown kiwi (apteryx mantelli) and screened for the presence of piroplasm dna using a nested-polymerase chain reaction (pcr) targeting the 18s rrna gene of most members of piroplasmida. all captive birds gave a negative pcr result, while 12 wild-caught birds were pcr positive. the nearly full-length 18s rrna gene for b. kiwiensis was sequenced. upon ... | 2008 | 18564765 |
| prevalence of babesia canis, borrelia afzelii, and anaplasma phagocytophilum infection in hard ticks removed from dogs in warsaw (central poland). | the purposes of this study were to specify the occurrence and prevalence of babesia canis, borrelia burgdorferi sensu lato, and anaplasma phagocytophilum in ticks removed from dogs in warsaw, and to determine the borrelia species occurring in ixodes ricinus ticks. among 590 collected ticks, 209 were identified as i. ricinus, and 381 as dermacentor reticulatus. dna of b. canis was detected in 11% of d. reticulatus ticks. we found that 6.2% of i. ricinus ticks harbored b. burgdorferi s.l. specific ... | 2008 | 18328630 |
| the scintigraphic evaluation of the pulmonary perfusion pattern of dogs hospitalised with babesiosis. | the possibility of coagulopathy in babesia canis rossi infections in the canine patient has been suggested in the literature, but minimal work has been done to evaluate the clinicopathological nature of it in further detail. pulmonary thromboembolism (pte) has not yet been implicated in canine babesiosis (cb), but may also be one of the causes of the sudden dyspnoea and tachypnoea that are frequently seen in complicated cb patients. the objective of this study was to prospectively evaluate the s ... | 2008 | 18846852 |
| [vector-borne parasites of dogs on the islands of cabo verde]. | during an animal welfare campaign on the islands of cape verde (in the capital praia on the island of santiago) a total of 57 dogs were examined for ectoparasites and blood parasites. 84% of the animals were infested with arthropods, mostly ticks. haemotropic parasites were examined via blood smear, polymerase chain reaction (pcr) and/or serology. 23 dogs had single infections, five multiple infections with haemoparasites. in eight dogs ehrlichia canis and in one amaplasma phagocytophilum were d ... | 2008 | 19066773 |
| [arthropod-borne parasites of dogs, especially leishmania, in the kosovo and albania]. | currently no information is available regarding canine arthropod-borne parasites in albania and the kosovo, especially the zoonotic protozoan leishmania infantum. presumably autochtonous cases of human leishmaniosis have been described for some areas (kosovo: ferizaj, gjakovo, pec, malisevo; albania: tirana, durres, elbasan, shkodra, vlore). in order to investigate the infection status of dogs of different origin sera from 272 animals (151 from albania - tirana, kamza and durres; 121 from the ko ... | 2008 | 19066774 |
| the application of pcr and reverse line blot hybridization to detect arthropod-borne hemopathogens of dogs and cats in trinidad. | arthropod-borne diseases are important causes of morbidity and mortality of companion animals in trinidad. as clinical signs are vague, more sensitive methods to diagnose these diseases based on the polymerase chain reaction (pcr) followed by reverse line blot hybridization (rlb) of amplified products are being developed. an rlb of 14 oligonucleotide probes coupled with polymerase chain-amplified regions of 16s rrna or 18s rrna genes of hemoparasites from cats and dogs detected ehrlichia canis, ... | 2008 | 19120209 |
| assessment of primers designed for the subspecies-specific discrimination among babesia canis canis, babesia canis vogeli and babesia canis rossi by pcr assay. | canine babesiosis is an infectious disease caused by either babesia gibsoni or babesia canis protozoans. the latter is also classified under three different phylogenetic groups, referred to as subspecies b. canis canis, b. canis vogeli and b. canis rossi. the objective of the present study was to validate and standardize a pcr assay to discriminate the organisms at the subspecies level. first, the reference sequences of the 18s rrna, 5.8s rrna and 28s rrna genes, including the internal transcrib ... | 2008 | 18242863 |
| prevalence of ehrlichia canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, bartonella vinsonii berkhoffii, and rickettsia spp. in dogs from grenada. | to identify the tick-borne pathogens in dogs from grenada, we conducted a serologic survey for ehrlichia canis in 2004 (104 dogs) and a comprehensive serologic and molecular survey for a variety of tick-borne pathogens in 2006 (73 dogs). in 2004 and 2006, 44 and 32 dogs (42.3% and 43.8%) were seropositive for e. canis, respectively. in 2006, several tick-borne pathogens were identified by serology and pcr. dna of e. canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, and bartonella ... | 2008 | 18160223 |
| plasma insulin concentrations in hypoglycaemic dogs with babesia canis rossi infection. | hypoglycaemia has been identified as a life-threatening metabolic complication in almost 20% of severely ill dogs suffering from babesiosis due to babesia canis rossi infection, and has been correlated with mortality. hyperinsulinaemia as a result of inappropriate insulin secretion may precipitate hypoglycaemia, and has been suggested as a possible cause of hypoglycaemia in human and murine malaria. this prospective, cross-sectional, observational study, including 94 dogs with naturally occurrin ... | 2008 | 18164550 |
| detection and molecular characterization of babesia canis vogeli from naturally infected dogs and rhipicephalus sanguineus ticks in tunisia. | canine babesiosis, caused by intra-erythrocytic babesia, is a tick-borne disease of worldwide importance. no information on canine babesiosis has been documented in tunisia. detection and analysis of babesia species from naturally infected dogs and ticks recovered from dogs were attempted by reverse line blot hybridization and nucleotide sequence analysis based on 18s rrna gene. out of 180 blood samples collected from domestic dogs in 4 villages situated in different bioclimatic zones, 12 were p ... | 2008 | 18242865 |
| molecular characterization of babesia canis canis isolates from naturally infected dogs in poland. | babesia canis has generally been considered the only large babesia to infect dogs. in this study, we used pcr to detect and characterize b. canis canis isolated from naturally infected dogs in poland by amplifying and sequencing a portion of the 18s ribosomal rna (rrna) gene. venous blood samples were collected from 76 babesia-symptomatic dogs. a 559-bp fragment of the b. canis canis 18s rrna gene was amplified by pcr. the pcr products were then digested with hincii restriction enzyme, and isola ... | 2008 | 18243559 |
| the brown dog tick, rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae): from taxonomy to control. | rhipicephalus sanguineus, commonly known as the brown dog tick, is a three-host tick that feeds primarily on dogs and occasionally on other hosts, including humans. r. sanguineus ticks are widely distributed around the world and they are known vectors of pathogens, such as babesia canis, ehrlichia canis, and rickettsia conorii. the increasing number of cases of human parasitism by r. sanguineus ticks reported in the literature indicates that the interaction between humans and r. sanguineus ticks ... | 2008 | 18280045 |
| an unusual form of canine babesiosis. | an akita inu, living in belgium, was presented with unusual clinical manifestations of acute babesiosis that included neurological signs and pancytopenia. diagnosis was made by identifying babesia canis in the blood smear. neurological signs resolved after treatment with imidocarb diproprionate. normalization of hematological abnormalities was gradual over 5 months. | 2008 | 18390102 |
| babesia canis canis and babesia canis vogeli infections in dogs from northern portugal. | canine babesiosis represents an important veterinary medical problem. this study describes the molecular characterization of babesial parasites detected in eight clinically suspected dogs from northern portugal, affected by lethargy, muscle tremors, weight loss, pale mucous membranes, hyperthermia or red-coloured urine. microscopic examination of peripheral blood smears showed large intraerythrocytic piroplasms morphologically compatible with babesia canis in all eight animals. dna was extracted ... | 2008 | 18602757 |
| a survey for infection with dirofilaria immitis, ehrlichia canis, borrelia burgdorferi, and babesia canis in feral and client-owned dogs in the turks and caicos islands, british west indies. | the frequency of infection with dirofilaria immitis and babesia canis and seropositivity to ehrlichia canis and borrelia burgdorferi in feral and client-owned dogs was determined. feral dogs were 14.8 and 11.2 times more likely to be seropositive to d. immitis and e. canis, respectively, than were client-owned dogs. none of the dogs tested positive for b. burgdorferi or b. canis. | 2008 | 18624070 |
| serum cardiac troponin i concentration in dogs with ehrlichiosis. | ehrlichiosis is a multisystemic disease with the potential to cause cardiomyocyte injury in naturally infected dogs. | 2008 | 18638021 |
| a pcr-based epidemiological survey of hepatozoon canis in dogs in nigeria. | the prevalence of hepatozoon canis infections in dogs in nigeria was surveyed using molecular methods. dna was extracted from blood samples obtained from 400 dogs. a primer set that amplified the babesia canis 18s rrna gene, which has high similarity to the h. canis 18s rrna gene, was used for the pcr. as a result, samples from 81 dogs (20.3%) produced 757 bp bands, which differed from the 698 bp band that corresponded to b. canis infection. the sequence of the pcr products of 10 samples were de ... | 2008 | 18685252 |
| [dogs babesiosis--still actually problem]. | babesiosis (piroplasmosis) is a tick-borne disease with a symptoms of hemolytic anemia. for the first time babesiosis was described in dogs in united states in 1934. the etiological factor of this disease in poland is protozoa babesia canis, and its vector--dermacentor-tick. the most common symptoms of babesiosis are: icterus, hemoglobinuria, occasionally vomits and diarrhea. the biochemical examination of blood serum from sick animals can reveal the increase of activity of ast, alt, the increas ... | 2008 | 18702315 |
| a study of cross-reactivity in serum samples from dogs positive for leishmania sp., babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test. | to verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by indirect fluorescent antibody (ifat) and enzyme-linked immunosorbent assay (elisa). all serum samples from endemic areas were positive for leishmania sp., by elisa and ifat, 51% positive for babesia canis and 43% for ehrlichia canis by ... | 2008 | 18554433 |
| first molecular characterization of babesia vogeli in two naturally infected dogs of buenos aires, argentina. | large piroplasms (>2.5microm) were detected by direct microscopical investigation in 34 out of 16,767 (0.20%) canine blood smears in the southern region of greater buenos aires. genomic dna was extracted from two parasitemic dogs and the hypervariable 18s rna gene region of the pathogen was specifically amplified, sequenced, and aligned with corresponding gene sequences available in the genbank. phylogenetic trees were constructed and compared. 18s rna gene sequences reliably segregated in three ... | 2008 | 18786765 |
| serological evidence for babesia canis infection of horses and an endemic focus of b. caballi in hungary. | in order to evaluate the seroconversion of horses to babesia caballi and b. canis in hungary, blood samples were collected from 371 animals on 23 different locations of the country. the presence of antibodies to b. caballi was screened with a competitive elisa. all 29 positive samples came from one region (the hortobágy). the prevalence of infection did not show correlation with sexes, and reached 100% in the age group of 2-5 years. babesia canis-specific antibodies were demonstrated by ifat in ... | 2007 | 18277708 |
| histological and ultrastructural studies of renal lesions in babesia canis infected dogs treated with imidocarb. | histological and electron microscopic examinations of the kidneys of 8 dogs suffering from fatal, naturally acquired babesia canis infection and nephropathy are presented. seven animals were treated with imidocarb dipropionate on average 4.5 days prior to death. severe anaemia was present only in 2 cases. degenerative histological changes observed mostly in the proximal convoluted tubules included vacuolar-hydropic degeneration, necrosis and detachment of renal tubular epithelial (rte) cells fro ... | 2007 | 18277710 |
| [a descriptive epidemiological study on canine babesiosis in the lake geneva region]. | a descriptive study was carried out in the district of the lake geneva between march 1, 2005 and august 31,2006 to assess the incidence and prevalence of canine babesiosis, to genotype the babesia species occurring, to assess the most frequently clinical signs found and to address the potential of autochthonous transmission. this included a data assessment on the different tick-populations occurring in the area and on the prevalence of babesia-dna in these ticks. a total of 56 veterinary practic ... | 2007 | 17983018 |
| molecular survey of babesia canis in dogs in nigeria. | an epidemiological study of babesia canis in dogs in nigeria was performed. four hundred blood samples collected from dogs in nigeria were investigated using nested pcr and sequence analysis. on nested pcr screening, nine samples (2.3%) produced a band corresponding to a 698-bp fragment indicative of b. canis infection. sequence analysis of the pcr products identified eight samples (2.0%) as b. canis rossi and the ninth (0.3%) as b. canis vogeli. this is the first report of the prevalence of b. ... | 2007 | 18057838 |
| pcr-rflp for the detection and differentiation of the canine piroplasm species and its use with filter paper-based technologies. | canine piroplasmosis is an emerging disease worldwide, with multiple species of piroplasm now recognised to infect dogs. a nested polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay was developed for the detection and differentiation of each of the piroplasm species currently known to infect dogs on the basis of the 18s ribosomal rna gene. the assay can potentially amplify and discriminate between theileria annae, theileria equi, babesia conradae, babesia gibsoni, ... | 2007 | 17127005 |
| babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen. | a cdna encoding the rhoptry-associated protein 1 (rap-1) homologue was obtained by immunoscreening an expression library prepared from babesia gibsoni merozoite mrna. the complete nucleotide sequence of the gene was 1740bp. computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kda. based on the sequence similarity, this putative protein was designated as the b. gibsoni rap-1 (bgrap-1). the bgrap-1 gene was ... | 2007 | 17127008 |
| epifaunistic arthropod parasites of the four-striped mouse, rhabdomys pumilio, in the western cape province, south africa. | flea, lice, mite, and tick species associated with 510 rhabdomys pumilio were collected at 9 localities in the western cape province, south africa. the aims of the study were first to quantify the species richness, prevalence, and relative mean intensity of infestation of epifaunistic arthropod species associated with r. pumilio, and second to determine temporal variations in the mean abundance of the parasitic arthropods. each mouse was examined under a stereoscopic microscope and its parasites ... | 2007 | 17436941 |
| endocrine predictors of mortality in canine babesiosis caused by babesia canis rossi. | this prospective, cross-sectional, observational study was designed to determine the association between the hormones of the pituitary-adrenal and pituitary-thyroid axes and outcome in dogs with naturally occurring babesia canis rossi babesiosis. ninety-five dogs with canine babesiosis were studied and blood samples were obtained from the jugular vein in each dog prior to treatment at admission to hospital. serum cortisol, adrenocorticotrophic hormone (acth), thyroxine, free thyroxine and thyrot ... | 2007 | 17614201 |
| coinfection with multiple tick-borne and intestinal parasites in a 6-week-old dog. | coinfection with ehrlichia canis, babesia canis, hepatozoon canis, isospora spp., giardia spp., and dipylidium caninum were detected in a 6-week-old dog. the effect of multi-pathogen infection was a fatal combination of gastrointestinal and hematologic abnormalities, including diarrhea, vomiting, anorexia, distended painful abdomen, intussusception, severe thrombocytopenia, anemia, and hypoproteinemia. | 2007 | 17616060 |
| first evidence of babesia gibsoni (asian genotype) in dogs in western europe. | canine babesiosis in europe is generally caused by babesia canis canis and babesia canis rossi. here we describe the first two autochthonous cases of babesia gibsoni (asian genotype) infection in germany. two american pit bull terriers showed clinical and hematologic signs consistent with babesiosis. polymerase chain reaction (pcr) analysis of the 18s rdna of blood samples revealed 486 bp fragments. the sequences were 100% identical to each other and 100% identical to babesia gibsoni (asian geno ... | 2007 | 17627433 |
| vaccination against large babesia species from dogs. | the original observation of sibinovic that soluble parasite antigens (spa) of b. canis could be used to protect dogs against challenge infection formed the starting point for the development of an effective vaccine. with the advent of in vitro cultivation techniques for haemoprotozoan parasites an important tool became available for the commercial production of the vaccine antigens. a first generation vaccine was developed for dogs, but it appeared that the level of protection induced was not co ... | 2007 | 17691601 |
| new advances in molecular epizootiology of canine hematic protozoa from venezuela, thailand and spain. | the prevalence of hematozoan infections (hepatozoon canis and babesia sp., particularly babesia canis vogeli) in canids from venezuela, thailand and spain was studied by amplification and sequencing of the 18s rrna gene. h. canis infections caused simultaneously by two different isolates were confirmed by rflp analysis in samples from all the geographic regions studied. in venezuela, blood samples from 134 dogs were surveyed. babesia infections were found in 2.24% of the dogs. comparison of sequ ... | 2007 | 17088022 |
| evidence of an acute phase response in dogs naturally infected with babesia canis. | the erythrocyte sedimentation rate (esr), white blood cell count (wbc), haematocrit (hct) and platelet number (plt) were quantified and compared with the acute phase proteins (apps) in dogs naturally infected with babesia canis and healthy dogs. both groups were treated with imidocarb dipropionate on the day of admission and both groups were monitored for all parameters on the admission day and on the first, second, third, fourth and seventh days in order to determine the presence of an acute ph ... | 2007 | 17116368 |
| immunity against babesia rossi infection in dogs vaccinated with antigens from culture supernatants. | soluble parasite antigens (spa) from different babesia species have been shown earlier to induce protective immunity when used as vaccine. however, initial attempts to produce such vaccine against babesia rossi infection using spa from b. rossi culture supernatants were not or only partially successful. here we show that when dogs were vaccinated with a vaccine comprising spa from b. rossi combined with spa from babesia canis protective immunity against experimental challenge infection was induc ... | 2007 | 17056181 |
| animal babesiosis: an emerging zoonosis also in italy? | in italy, babesiosis is widespread in several central and southern regions, but few data are available on its presence in most italian areas. in 2004 a project was financed by the miur to investigate on the babesiosis epidemiology in vertebrate and invertebrate hosts, and on the transmission risk for humans in central and northern regions of the country. microscopy and/or molecular tools were applied to blood samples of wild animals, livestock and pets, and to 1,677 ticks collected on animals or ... | 2007 | 17691605 |
| a field trial evaluation of the prophylactic efficacy of amitraz-impregnated collars against canine babesiosis (babesia canis rossi) in south africa. | south african canine babesiosis caused by babesia canis rossi is a common clinical disease in dogs in south africa and remains a significant cause of domestic dog mortality. to determine whether tick-repellent, 9% amitraz-impregnated tick collars (preventic-virbac) could prevent tick-borne exposure to b. canis rossi, 50 dogs were assigned to two groups. group 1 (20 dogs), polymerase chain reaction (pcr)--and reverse line blot (rlb)-negative for b. canis rossi, were fitted with amitraz collars an ... | 2007 | 17941596 |
| the response of the pituitary-adrenal and pituitary-thyroidal axes to the plasma glucose perturbations in babesia canis rossi babesiosis. | this prospective, cross-sectional, interventional study was designed to determine the association between the hormones of the pituitary-adrenal and pituitary-thyroid axes and other clinical parameters with the blood glucose perturbations in dogs with naturally occurring babesia canis rossi babesiosis. thirty-six dogs with canine babesiosis were studied. blood samples were obtained from the jugular vein in each dog prior to treatment at admission to hospital and serum endogenous adrenocorticotrop ... | 2007 | 18507221 |
| [importance of pcr for the diagnostics of canine babesiosis]. | clinical standards to confirm babesiosis in dogs include the direct identification of the infectious agent in blood smears and serological assays for babesia canis-specific antibodies. here, we demonstrate in seven cases (with data on anamnesis, clinics, laboratory diagnostics, and therapeutic outcomes) that a new diagnostic procedure is required. this is the molecular-genetic identification of babesia by real time pcr allowing an unequivocal identification of the infectious agents. indeed, all ... | 2006 | 17263080 |
| clinical manifestations of canine babesiosis in hungary (63 cases). | clinical observations of babesia canis infection in 63 dogs during a 1-year period are summarised, demonstrating the pathogenicity of the babesia strain endemic in hungary. most patients had babesiosis in the spring and autumn, correlating with the seasonal activity of ticks. male animals appeared in higher numbers, probably due to an overrepresentation of outdoor dogs. uncomplicated babesiosis was diagnosed in 32 cases. the disease affected dogs of any age in this study. symptoms were similar t ... | 2006 | 17020140 |
| detection of anaplasma platys and babesia canis vogeli and their impact on platelet numbers in free-roaming dogs associated with remote aboriginal communities in australia. | to detect anaplasma platys and babesia canis vogeli infection, using polymerase chain reaction (pcr)-based assays, in free-roaming dogs associated with eight aboriginal communities in remote areas of australia and to determine the impact of infection through the assessment of platelet numbers. | 2006 | 16958629 |
| occurrence of hard ticks in dogs from warsaw area. | ticks are known as a vector of many viral, bacterial, rickettsial and protozoan infections of dogs. the threat with particular tick-transmitted disease depends very much on the tick species which feeds on the dog. in this paper we present results of research designed to identify tick species which attack dogs in the warsaw area. ticks were collected from dogs presented for medical examination in veterinary clinics in different parts of warsaw. all of 590 tick specimens found on dogs belonged to ... | 2006 | 17196014 |
| clinicopathological changes and effect of imidocarb therapy in dogs experimentally infected with babesia canis. | in this study one spleen-intact dog (a) and two splenectomised dogs (bse, cse) were infected with babesia canis. all animals developed an acute disease characterised by fever, haemoglobinuria and anaemia, the latter being more severe in the splenectomised dogs. fever and parasitised red blood cells were detected for three days after imidocarb treatment in the splenectomised animals. haematological abnormalities included regenerative anaemia, thrombocytopenia and leukopenia (due to neutropenia an ... | 2006 | 16613023 |
| capillary and venous babesia canis rossi parasitaemias and their association with outcome of infection and circulatory compromise. | this observational study of 100 dogs naturally infected with babesia canis rossi determined whether severity of parasitaemia was associated with outcome of infection and documented the relative distribution of parasitised red blood cells (prbc) in capillary and venous circulation. the association between increased parasitaemias and outcome with a clinically compromised circulation was also investigated. outcome was defined as either hospitalisation with death, or hospitalisation with eventual re ... | 2006 | 16806713 |
| onset and duration of immunity against babesia canis infection in dogs vaccinated with antigens from culture supernatants. | it has previously been shown that dogs can be vaccinated against heterologous babesia canis infection using a vaccine containing soluble parasite antigens (spa) from in vitro cultures of b. canis and b. rossi that are adjuvanted with saponin. in the present study the onset and duration of immunity of vaccinated dogs were studied. results showed that 3-26 weeks after initial vaccination, dogs effectively limit the level of spa in plasma upon challenge infection, which was reflected in limited dur ... | 2006 | 16504401 |
| piroplasma infection in dogs in northern spain. | babesiosis is a world-wide zoonosis caused by tick-borne hematozoan parasites of the genus babesia. canine babesidae have historically been classified as "large babesia" (babesia canis) and "small babesia" (babesia gibsoni) based on the size of their intraerythrocytic forms. genetic sequencing technology using the polymerase chain reaction (pcr) has allowed further subdivision. b. gibsoni has three strains: "asia", "california" and a recently identified small babesial-like parasite, theileria an ... | 2006 | 16513282 |
| babesia canis vogeli: a novel pcr for its detection in dogs in australia. | babesia canis vogeli is known to cause disease in dogs in australia, and the rapid detection of various subspecies would enable effective treatment and management. a 21 bp oligonucleotide, "bab-f" was proposed for the production of larger pcr products with high species specificity that would enable effective sequence analyses to yield subspecies identification. the new forward primer when paired with a previously reported "babesia common" reverse primer generated a 394 bp product which was succe ... | 2006 | 16256109 |
| the south african form of severe and complicated canine babesiosis: clinical advances 1994-2004. | babesia (canis) rossi infection is common in dogs in south africa, and frequently causes severe, life-threatening disease. acidemia, persistent hyperlactatemia, hemoconcentration, elevated creatinine, cerebral babesiosis, pulmonary edema and pancreatitis are all associated with mortality rates above 30%, compared with overall mortality of 12% in admitted cases. although half the admitted cases are severely anemic, hemoconcentration is associated with far higher mortality. cerebral babesiosis is ... | 2006 | 16503090 |
| the low seroprevalence of tick-transmitted agents of disease in dogs from southern ontario and quebec. | infectious diseases caused by pathogens transmitted by ticks and other insect vectors are an important cause of morbidity and mortality in both dogs and humans throughout north america. the purpose of this study was to determine the seroprevalence of selected vector-transmitted pathogens in southern ontario and quebec. samples submitted to the vector borne disease diagnostic laboratory (vbddl) at the north carolina state university college of veterinary medicine were evaluated for antibodies to ... | 2006 | 17217089 |
| evidence for an increased geographical distribution of dermacentor reticulatus in germany and detection of rickettsia sp. rpa4. | two studies were performed to elucidate the current distribution of the tick dermacentor reticulatus in germany. in the first one in 2003, a total of 365 dogs from 171 sites in the states of berlin and brandenburg was screened for ticks, and the corresponding outdoor sites that the dogs usually visited were searched for host-seeking ticks by the flagging method. a total of 1155 ticks was removed from the dogs. the majority were ixodes ricinus (88.5%), followed by d. reticulatus (9.1%) and i. hex ... | 2006 | 16524777 |
| genetic basis for gpi-anchor merozoite surface antigen polymorphism of babesia and resulting antigenic diversity. | glycosyl-phosphatidylinositol anchor merozoite surface antigens (gpi-anchor msa) are proposed to act in the invasion process of infective merozoites of babesia into host erythrocytes. because of their essential function in the survival of babesia parasites, they constitute good candidates for the development of vaccines against babesiosis and they have been extensively analyzed. these include babesia bovis variable msa (vmsa) and babesia bigemina gp45/gp55 proteins of the agents of bovine babesi ... | 2006 | 16551492 |
| what is your diagnosis? babesia canis and canine distemper virus. | 2006 | 16573768 | |
| [evaluation of ectoparasites and hemoparasites in dogs kept in apartments and houses with yards in the city of juiz de fora, minas gerais, brazil]. | fleas and ticks transmit various pathogens while feeding on the blood of dogs. this study sought to verify the occurrence of ectoparasitism and hemoparasitism in dogs from two urban areas in the city of juiz de fora, minas gerais, brazil. between february and august 2003, 101 dogs were studied: 50 came from apartments in the downtown region and 51 from houses with grassy yards. the ectoparasites were collected and conserved in etanol 70%. the occurrence of hemoparasites was verified by examining ... | 2006 | 16646996 |
| seroprevalence of canine babesiosis in hungary suggesting breed predisposition. | six hundred fifty-one blood samples were collected from urban and rural dogs in various parts of hungary to measure antibody levels to babesia canis with indirect fluorescent antibody test. thirty-seven (5.7%) of the sera showed positivity with titers between 1:80 and 1:10,240. seroconverted dogs were found in 13 locations of the country. it is concluded that canine babesiosis is becoming more prevalent in eastern hungary. seropositivity increased then declined with age, reaching a maximum in ca ... | 2006 | 16715235 |
| tick-transmitted diseases in dogs: clinicopathological findings. | in this article we describe the main clinicopathological findings of some tick-transmitted diseases observed in italy, due to ehrlichia canis and babesia canis, and most rarely anaplasma phagocytophilum and anaplasma platys. canine monocytic ehrlichiosis (cme) is a multisystemic disorder that is characterized by various clinical signs. acutely-infected dogs show various clinical and haematological abnormalities including fever, lymphadenopathy, anorexia, lethargy, depression and thrombocytopenia ... | 2006 | 16881415 |
| dog tick-borne diseases in sicily. | in sicily many tick borne diseases are endemic, in particular way those that see like main carrier ticks that prefer, for their vital cycle, climatic conditions characterized by high temperatures and a warmth-humid atmosphere. the more important pathologies transmitted by ticks causing diseases in dogs are babesiosis and ehrlichiosis. borrelia burgdorferi, anaplasma phagocytophilum, rickettsia conorii, coxiella burnetii and tick transmitted encephalitis virus assume particular relevance because ... | 2006 | 16881419 |
| new data on epizootiology and genetics of piroplasms based on sequences of small ribosomal subunit and cytochrome b genes. | as a continuation of our studies on molecular epizootiology of piroplasmosis in spain and other countries, we present in this contribution the finding of new hosts for some piroplasms, as well as information on their 18s rrna gene sequences. genetic data were complemented with sequences of apocytochrome b gene (whenever possible). the following conclusions were drawn from these molecular studies: theileria annulata is capable of infecting dogs, since it was diagnosed in a symptomatic animal. acc ... | 2006 | 16919391 |
| canine babesiosis: a brazilian perspective. | canine babesiosis is highly endemic in brazil, caused by babesia canis and babesia gibsoni, both transmitted by rhipicephalus sanguineus ticks. the present review argues for a more adequate method of characterizing the babesia species infecting dogs and cats in different brazilian endemic zones. it advocates for a comprehensive understanding of the biology of r. sanguineus ticks under brazilian conditions in order to define the more effective preventive strategies against canine babesiosis and c ... | 2006 | 16962707 |
| [dermacentor reticulatus (fabricius, 1794)--distribution, biology and vector for babesia canis in germany]. | this work deals with occurrence, distribution as well as biology and vectorial capacity of the ornate dog tick (dermacentor (d.) reticulatus). until 30 years ago this tick has only been described in a few natural foci in southern germany. its distribution has however expanded in the course of the last years. with the exception of northern germany it is now covering large areas of germany. this is particularly the case in the upper rhine valley, saarland and the mark brandenburg. however, it is e ... | 2006 | 17009718 |
| spatial distribution of dermacentor reticulatus and ixodes ricinus in hungary: evidence for change? | a survey was conducted to investigate the spatial distribution of ixodes ricinus and dermacentor reticulatus in hungary and to compare these data with the results of a previous large-scale survey. in the survey conducted in the 1950s, d. reticulatus adults were detected in two isolated areas of two counties, and the presence of these ticks in the collection was explained by accidental introduction. in the present survey, d. reticulatus became the second most common species occurring in all 16 co ... | 2005 | 15740873 |
| [autochthonous cases of canine babesiosis in the canton solothurn]. | starting in november 2003 a series of five clinical cases of canine babesiosis was registered in the region of obergösgen (canton solothurn). all presented dogs showed increased body temperature, thrombocytopenia and hemoglobinuria, and none of the dogs had been abroad or visited endemic regions in the southern or western part of switzerland so far. babesia canis was detected in the blood smears of all five patients, but only three had detectable specific antibodies against this parasite. howeve ... | 2005 | 15999636 |
| babesia dna detection in canine blood and dermacentor reticulatus ticks in southwestern siberia, russia. | babesia infection was studied in 21 blood samples of dogs with symptoms of babesiosis and among 72 dermacentor reticulatus and 70 ixodes persulcatus ticks from southwestern siberia, russia. babesia dna was detected by hemi-nested pcr based on the 18s rrna gene with subsequent direct sequencing. all of the analyzed canine blood samples and three d. reticulatus, but none from i. persulcatus ticks studied were shown to contain babesia dna. nucleotide sequences of the babesia 18s rrna gene fragment ... | 2005 | 16187898 |
| detection of babesia canis rossi, b. canis vogeli, and hepatozoon canis in dogs in a village of eastern sudan by using a screening pcr and sequencing methodologies. | babesia and hepatozoon infections of dogs in a village of eastern sudan were analyzed by using a single pcr and sequencing. among 78 dogs, 5 were infected with babesia canis rossi and 2 others were infected with b. canis vogeli. thirty-three dogs were positive for hepatozoon. hepatozoon canis was detected by sequence analysis. | 2005 | 16275954 |
| tickborne pathogen detection, western siberia, russia. | ixodes persulcatus (n = 125) and dermacentor reticulatus (n = 84) ticks from western siberia, russia, were tested for infection with borrelia, anaplasma/ehrlichia, bartonella, and babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. i. persulcatus ticks were infected with borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), anaplasma phagocytophilum (2.4% +/- 1.4%), ehrlichia muris (8.8% +/- 2.5%), and bartonella spp. (37.6% +/- 4.3%). d. re ... | 2005 | 16318722 |
| vaccination against canine babesiosis. | it has been known for several decades that the soluble parasite antigen (spa) of several babesia species can be used as a vaccine against the clinical manifestations of babesiosis. originally observed in the plasma of infected animals, spa can also be recovered from the supernatants of in vitro cultures of these parasites. variable success has been reported for vaccines against the bovine and canine babesia parasites, which seems to be related to antigenic diversity within babesia species. in th ... | 2005 | 15780840 |
| autochthonous canine babesiosis in the netherlands. | outbreaks of autochthonous babesiosis, caused by babesia canis, occurred in the netherlands in the spring and autumn of 2004 affecting 23 dogs. nineteen animals recovered after treatment, whereas four dogs died. adult dermacentor reticulatus ticks collected from these dogs indicate that canine babesiosis could become endemic in the netherlands. | 2005 | 15923085 |
| geographic distribution of babesiosis among dogs in the united states and association with dog bites: 150 cases (2000-2003). | to identify the geographic distribution of babesiosis among dogs in the united states and determine, for dogs other than american pit bull terriers (apbts), whether infection was associated with a recent dog bite. | 2005 | 16190594 |
| serum concentration of some acute phase proteins in naturally occurring canine babesiosis: a preliminary study. | serum concentrations of acute phase proteins can provide valuable diagnostic information in the detection, prognosis, or monitoring of disease. information available on the acute phase response in naturally occurring canine babesiosis is limited. | 2005 | 15902667 |
| detection and molecular characterization of a novel large babesia species in a dog. | babesia canis has generally been considered the only large babesia to infect dogs. here we describe the molecular characterization of a large babesia species that was detected in the blood and bone marrow of a dog with clinical and hematological abnormalities consistent with babesiosis. analysis of the 18s rrna genes revealed a unique sequence that shared 93.9% sequence identity with b. bigemina and 93.5% sequence identity with b. caballi, compared to 91.2-91.6% identity with b. canis canis, b. ... | 2004 | 15381295 |
| molecular fluorescent approach to assessing intraerythrocytic hemoprotozoan babesia canis infection in dogs. | the development of recent flow cytometry-based protocols for the diagnosis of canine babesiosis, babesia gibsoni in particular, has encouraged us to investigate its applicability to detect b. canis-infected erythrocytes as well as optimize the hydroethidine-flow cytometry methodology (he-fc), using peripheral blood samples from naturally and experimentally infected dogs. our data demonstrated that he at 25 microg/ml provided the most outstanding fluorescence profile, able to discriminate between ... | 2004 | 15482880 |
| canine babesiosis in slovenia: molecular evidence of babesia canis canis and babesia canis vogeli. | canine babesiosis, caused by intraerythrocytic babesia spp., is a tick-borne disease of worldwide importance. no information on canine babesiosis has been documented in slovenia. therefore, 238 dogs admitted to the small animal clinic in ljubljana from the years 2000 to 2002 were tested for the presence of babesial parasites in the blood. based on clinical, microscopic and molecular investigations, 14 dogs (5.9%) were determined as being infected with babesiae. clinical signs relating to acute h ... | 2004 | 15210084 |
| prevalence and risk factors of hypoglycemia in virulent canine babesiosis. | hypoglycemia is a common complication of virulent canine babesiosis. a study was conducted to determine the prevalence of and potential risk factors for hypoglycemia in canine babesiosis from babesia canis rossi. plasma glucose concentration was measured at presentation in 250 dogs with babesiosis, of which 111 were admitted to hospital. the prevalence of hypoglycemia (<60 mg/dl) was 9% (23/250). twenty-two hypoglycemic dogs required admission, making the prevalence of hypoglycemia in admitted d ... | 2004 | 15188810 |
| serodiagnosis of canine babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant p50 expressed in escherichia coli. | the entire p50 gene encoding a surface protein of babesia gibsoni was cloned into the bacteria expression vector pgex-4t-3 and subsequently expressed in escherichia coli as a glutathione s-transferase fusion protein. the purified recombinant p50 was evaluated in an enzyme-linked immunosorbent assay (elisa) for the serological diagnosis of b. gibsoni infection in dogs. elisa was able to differentiate clearly among b. gibsoni-infected, babesia canis-infected, and uninfected dog sera. the antibody ... | 2004 | 15165064 |
| free light-chain proteinuria and normal renal histopathology and function in 11 dogs exposed to lleishmania infantum, ehrlichia canis, and bbabesia canis. | the purpose of this retrospective study was to investigate the relationship among proteinuria consisting of immunoglobulin free light chains (flcs), renal histopathologic findings, and routine markers of renal function in 11 dogs exposed to leishmania infantum (n = 8), ehrlichia canis (n = 2), and babesia canis (n = 1). flc proteinuria was suspected based on identification of a 22- to 27-kda band by sodium dodecyl sulfate-agarose gel electrophoresis (sds-age) and later confirmed by immunofixatio ... | 2004 | 15515575 |
| retrospective study (1998-2001) on canine babesiosis in belo horizonte, minas gerais, brazil. | the present work describes a retrospective study of clinical cases of babesiosis in dogs examined at the veterinary hospital (universidade federal de minas gerais) from march 1998 to september 2001. from the clinical records of dogs with laboratory-confirmed babesia canis infections, we analyzed: demography (age, breed, sex, time of year, and origin; clinical manifestations (concomitant infections, body temperature, presence of ticks, and clinical signs); and hematological alterations. from 194 ... | 2004 | 15604486 |
| [ehrlichia and babesia infections in dogs in the netherlands]. | a retrospective study was performed at the department of clinical sciences of companion animals at utrecht university amongst 75 dogs diagnosed with a babesia canis and/or an ehrlichia canis infection. the majority of the dogs had visited an endemic area (most often the mediterranean area or the dutch antilles), but two dogs became infected with babesia in the netherlands. babesia infections were associated with a stay in an endemic area and an incubation period that are both significantly short ... | 2004 | 15622893 |
| are urea and creatinine values reliable indicators of azotaemia in canine babesiosis? | serum urea and creatinine are extensively used as parameters to screen for azotaemia. their reciprocal plots roughly correlate with glomerular filtration rate (gfr). they are, however, subject to influence by non-renal factors and to increase their specificity they are often tested concurrently. in renal disease they are expected to behave similarly, with both parameters increasing as gfr decreases. haemolysis, as it occurs in canine babesiosis, may cause non-renal elevations in serum urea, poss ... | 2004 | 15628803 |
| babesia canis canis in dogs from hungary: detection by pcr and sequencing. | canine babesiosis in hungary has always been a severe and frequent disease, attributed to infection with babesia canis transmitted by dermacentor reticulatus. identification of the disease agent has been based merely on size and morphology of the intraerythrocytic parasites and no evidence has been found concerning the subspecies (genotype) of b. canis. therefore, a molecular survey on natural babesia infection of dogs in hungary using pcr and sequence analysis was attempted to clarify the subsp ... | 2004 | 15710522 |
| electron microscopic study of canine babesia gibsoni infection. | canine babesiosis is a tick-borne parasitic disease caused by the intraerythrocytic parasites, babesia canis and babesia gibsoni. a lethargic, weak, american staffordshire terrier (pit bull) dog, which had regenerative, normocytic, normochromic anemia, was shown by polymerase chain reaction analysis to be infected with b. gibsoni. transmission electron microscopy of ethylenediamine tetraacetic acid-treated blood disclosed many well-preserved, intraerythrocytic babesia trophozoites. four morpholo ... | 2004 | 15152839 |
| infection with a proposed new subspecies of babesia canis, babesia canis subsp. presentii, in domestic cats. | parasitemia with a large babesia species was identified in two domestic cats from israel. one cat, also coinfected with feline immunodeficiency virus and "candidatus mycoplasma haemominutum," had profound icterus and anemia which resolved after therapy, whereas a second cat was an asymptomatic carrier. amplification and sequencing of the 18s rrna gene, followed by phylogenetic analyses, indicated that infection was caused by babesia canis. however, the sequences of the internal transcribed and 5 ... | 2004 | 14715738 |
| identification of a new type of babesia species in wild rats (bandicota indica) in chiang mai province, thailand. | a new type of rodent babesia, which resembled babesia microti but was phylogenetically placed closest, with the highest level of statistical support, to babesia canis, a canine babesia, was identified in thai bandicota indica in thai provinces to which malaria is endemic. close watch should be kept on human babesiosis in thailand. | 2004 | 14766871 |
| confirmation of occurrence of babesia canis vogeli in domestic dogs in south africa. | the prevalence of babesia infections in domestic dogs in south africa was studied using reverse line blot hybridization and 18s sequence analysis. babesia canis vogeli was confirmed for the first time in domestic dogs in south africa. out of a total of 297 blood samples collected from domestic dogs in bloemfontein, east london, johannesburg, durban and from the onderstepoort veterinary academic hospital, 31 were positive for babesia canis rossi, whereas b. c. vogeli was detected in 13 dogs. none ... | 2004 | 15177716 |
| infection and blood transfusion: a guide to donor screening. | in recent years, blood-component therapy has become more accessible in veterinary practice. as with human medicine, care must be taken to minimize the risk of disease transmission from donor to recipient. determining the appropriate diseases to screen for is complicated by regional variations in disease incidence, the existence of chronic carrier states for some diseases, the difficulty in screening-test selection, and testing cost. the feline diseases considered include retroviral infections, f ... | 2004 | 15179926 |