Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| an entamoeba histolytica adp-ribosyl transferase from the diphtheria toxin family modifies the bacterial elongation factor tu. | adp-ribosyl transferases are enzymes involved in the post-translational modification of proteins; they participate in multiple physiological processes, pathogenesis and host-pathogen interactions. several reports have characterized the functions of these enzymes in viruses, prokaryotes and higher eukaryotes, but few studies have reported adp-ribosyl transferases in lower eukaryotes, such as parasites. the locus ehi_155600 from entamoeba histolytica encodes a hypothetical protein that possesses a ... | 2016 | 27234208 |
| proteomic study of entamoeba histolytica trophozoites, cysts, and cyst-like structures. | the cyst stage of entamoeba histolytica is a promising therapeutic target against human amoebiasis. our research team previously reported the production in vitro of cyst-like structures (cls) sharing structural features with cysts, including rounded shape, size reduction, multinucleation, and the formation of a chitin wall coupled to the overexpression of glucosamine 6-phosphate isomerase, the rate-limiting enzyme of the chitin synthesis pathway. a proteomic study of e. histolytica trophozoites, ... | 2016 | 27228164 |
| diagnostic performances of antigen detection compared to conventional and nucleic acid detection of entamoeba histolytica in a non-endemic setting. | this study evaluated the immunochromatographic (ic) assay "techlab(®) e. histolytica quik chek™" analysing 36 faecal samples and 7 cultured strains. this assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. the ic assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity with other parasites and failed to detect e. histolytica antigen in frozen faeces. in our laboratory located in a non-endemic setting this assay could not rep ... | 2016 | 27196557 |
| amoebiasis vaccine development: a snapshot on e. histolytica with emphasis on perspectives of gal/galnac lectin. | amoebiasis/amebiasis is a gastrointestinal infection caused by an enteric dwelling protozoan, entamoeba histolytica. the disease is endemic in the developing world and is transmitted mainly via the faecal-oral route (e.g., in water or food) and may or may not be symptomatic. this disease of socio-economic importance worldwide involves parasite adherence and cytolysis of human cells followed by invasion that is mediated by galactose-binding (gal/galnac) surface lectin. disruption of the mucus lay ... | 2016 | 27181579 |
| cryptosporidium and other intestinal parasitic infections among hiv patients in southern ethiopia: significance of improved hiv-related care. | intestinal parasitic infections are known to cause gastroenteritis, leading to higher morbidity and mortality, particularly in people living with hiv/aids. this study aimed to determine the prevalence of cryptosporidium and other intestinal parasitic infections among hiv patients receiving care at a hospital in ethiopia where previous available baseline data helps assess if improved hiv-related care has reduced infection rates. | 2016 | 27165271 |
| detection of beta-tubulin in the cytoplasm of the interphasic entamoeba histolytica trophozoites. | it is known that the microtubules (mt) of entamoeba histolytica trophozoites form an intranuclear mitotic spindle. however, electron microscopy studies and the employment of anti-beta-tubulin (β-tubulin) antibodies have not exhibited these cytoskeletal structures in the cytoplasm of these parasites. the purpose of this work was to detect β-tubulin in the cytoplasm of interphasic e. histolytica trophozoites. activated or non-activated hmi-imss-strain e. histolytica trophozoites were used and cult ... | 2016 | 27156446 |
| species-specific immunodetection of an entamoeba histolytica cyst wall protein. | entamoeba histolytica causes intestinal disease in endemic settings throughout the world. diagnosis of e. histolytica infection would be improved by the identification of biomarkers that are expressed by cysts of e. histolytica, but not by cysts of closely related commensal species of entamoeba. herein, we describe two novel monoclonal antibodies (1a4 and 1d3) produced against a spacer region of the e. histolytica jacob2 lectin, an outer cyst wall protein. these reagents demonstrated no cross-re ... | 2016 | 27152855 |
| entamoeba histolytica induces human neutrophils to form nets. | entamoeba histolytica invades the intestine and other organs during the pathogenesis of amoebiasis. in the early stages, the host organism responds with an inflammatory infiltrate composed mostly of neutrophils. it has been reported that these immune cells, activated by e. histolytica, exert a protective role by releasing proteolytic enzymes and generating reactive oxygen/nitrogen species (ros/rns) and antimicrobial peptides. it is now known that neutrophils also produce neutrophil extracellular ... | 2016 | 27138813 |
| gastrointestinal parasitosis: histopathological insights to rare but intriguing lesions of the gastrointestinal tract. | gastrointestinal parasitosis is a significant cause of morbidity and mortality. definitive diagnosis is usually made by stool tests and/or serology but may require tissue evaluation. although pathologists are usually familiar with common parasites, it is not well established whether the diagnosis could be suspected without seeing the "parasite" itself. | 2016 | 27136106 |
| archaeological occurrences and historical review of the human amoeba, entamoeba histolytica, over the past 6000years. | understanding parasite history and the evolution of host/parasite relationships is one of the most important aspects of paleoparasitology. within the framework of this research topic, this paper focuses on the human pathogenic amoeba, entamoeba histolytica. the compilation of all the available archaeological data concerning this parasite leads to a first glimpse of the history of this parasite of current medical importance. paleoparasitological investigation into this parasite uses immunological ... | 2016 | 27130884 |
| trends in prevalence of soil-transmitted helminth and major intestinal protozoan infections among school-aged children in nepal. | to assess the trends in prevalence of soil-transmitted helminths (sths), entamoeba histolytica and giardia lamblia among school-aged children in nepal between 1990 and 2015. | 2016 | 27097973 |
| the motility of entamoeba histolytica: finding ways to understand intestinal amoebiasis. | the pathogenic amoeba entamoeba histolytica is able to migrate within various compartments of the human body. the present article reviews progress in understanding the mechanisms of cell motility in e. histolytica during human intestinal invasion and, in particular, how the three-dimensional characteristics of the environment regulate the parasite's behaviour. the amoeboid mode of migration that applies to e. histolytica's displacements on two-dimensional surfaces is also expected to apply to th ... | 2016 | 27497052 |
| n-acetyl ornithine deacetylase is a moonlighting protein and is involved in the adaptation of entamoeba histolytica to nitrosative stress. | adaptation of the entamoeba histolytica parasite to toxic levels of nitric oxide (no) that are produced by phagocytes may be essential for the establishment of chronic amebiasis and the parasite's survival in its host. in order to obtain insight into the mechanism of e. histolytica's adaptation to no, e. histolytica trophozoites were progressively adapted to increasing concentrations of the no donor drug, s-nitrosoglutathione (gsno) up to a concentration of 110 μm. the transcriptome of no adapte ... | 2016 | 27808157 |
| identification of entamoeba histolytica and e. dispar infection in maceió, alagoas state, northeast brazil. | a cross-sectional study was carried out to assess the prevalence of e. histolytica and e. dispar by examining stool samples obtained from 1,003 students of public schools in maceió, alagoas, brazil. | 2016 | 27801380 |
| recent progress on the discovery of antiamoebic agents. | a large number of protozoans infect humans but entamoeba histolytica is the only organism responsible for causing amoebiasis, a deadly disease after malaria. numerous heterocycle-based antiamoebic agents have been previously synthesized as e. histolytica inhibitors and while some of these agents have shown moderate activity, the search for a novel and ideal antiamoebic compound is still ongoing. in this digest letter, we present the latest data on antiamoebic agents from 2011 to 2016 based on th ... | 2016 | 27707603 |
| a new nucleocytoplasmic rhogap protein contributes to control the pathogenicity of entamoeba histolytica by regulating ehracc and ehracd activity. | small gtpases are signalling molecules that regulate important cellular processes. gtpases are deactivated by gtpase-activating proteins (gaps). while human gaps have been intensively studied, no gap has yet been characterized in entamoeba histolytica. in this study, we identified and characterized a novel nucleocytoplasmic rhogap in e. histolytica termed ehrhogapnc. in silico analyses of the domain structure revealed a previously undescribed peptide region within the carboxy-terminal region of ... | 2016 | 27107405 |
| cardioprotective effects of monocyte locomotion inhibitory factor on myocardial ischemic injury by targeting vimentin. | monocyte locomotion inhibitory factor (mlif), a heat-stable pentapeptide produced by entamoeba histolytica, has anti-inflammatory function and protective effect on ischemic stroke. in this study, we evaluated the effect of mlif on myocardial ischemia. mice were subjected to ischemia/reperfusion by occlusion of the left anterior descending artery (lad). after sacrifice, the serum concentrations of cardiac troponin i (ctni), creatine kinase (ck), lactate dehydrogenase (ldh) as well as the heart in ... | 2016 | 27773717 |
| extensive transcriptome analysis correlates the plasticity of entamoeba histolytica pathogenesis to rapid phenotype changes depending on the environment. | amoebiasis is a human infectious disease due to the amoeba parasite entamoeba histolytica. the disease appears in only 20% of the infections. diversity in phenotypes may occur within the same infectious strain in the gut; for instance, parasites can be commensal (in the intestinal lumen) or pathogenic (inside the tissue). the degree of pathogenesis of clinical isolates varies greatly. these findings raise the hypothesis that genetic derivation may account for amoebic diverse phenotypes. the main ... | 2016 | 27767091 |
| role of ehrab7a in phagocytosis of type 1 fimbriated e. coli by entamoeba histolytica. | entamoeba histolytica, the causative agent of amoebic colitis and liver abscess in human, ingests the intestinal bacteria and variety of host cells. phagocytosis of bacteria by the amebic trophozoite has been reported to be important for the virulence of the parasite. here, we set out to characterize different stages of phagocytosis of type 1 e. coli and investigated the role of a set of amoebic rab gtpases in the process. the localizations of the rab gtpases during different stages of the phago ... | 2016 | 27663892 |
| entamoeba encystation: new targets to prevent the transmission of amebiasis. | amebiasis is caused by entamoeba histolytica infection and can produce a broad range of clinical signs, from asymptomatic cases to patients with obvious symptoms. the current epidemiological and clinical statuses of amebiasis make it a serious public health problem worldwide. the entamoeba life cycle consists of the trophozoite, the causative agent for amebiasis, and the cyst, the form responsible for transmission. these two stages are connected by "encystation" and "excystation." hence, develop ... | 2016 | 27764256 |
| efficiency of direct microscopy of stool samples using an antigen-specific adhesin test for entamoeba histolytica. | e. histolytica is among the common causes of acute gastroenteritis. the pathogenic species e. histolytica and the nonpathogenic species e. dispar cannot be morphologically differentiated, although correct identification of these protozoans is important for treatment and public health. in many laboratories, the screening of leukocytes, erythrocytes, amoebic cysts, trophozoites and parasite eggs is performed using native-lugol's iodine for pre-diagnosis. | 2016 | 27761283 |
| recurrent amebic liver abscesses over a 16-year period: a case report. | amebic liver abscess is a rare disease in high-income countries. recurrence of amebic liver abscess is even rarer with only a few previous reports. here we present a patient who developed three subsequent amebic liver abscesses over a sixteen-year period. | 2016 | 27756400 |
| prevalence of intestinal parasitic infections and associated risk factors among schoolchildren in the plateau central and centre-ouest regions of burkina faso. | unsafe drinking water, unimproved sanitation and lack of hygiene pose health risks, particularly to children in low- and middle-income countries. this study aimed to assess the prevalence and risk factors of intestinal parasitic infections in school-aged children in two regions of burkina faso. | 2016 | 27756339 |
| the potential of lactobacillus casei and entercoccus faecium combination as a preventive probiotic against entamoeba. | travellers' diarrhoea caused by enteric protozoa like entamoeba histolytica is among the most common protozoan diseases in developing countries. in developing countries, amoebiasis is the second most prevalent protozoan disease. this protozoan parasite is often known to coexist as a part of the normal gut microbiota. it is estimated that around 50-60 % of population in developing countries might be harbouring entamoeba in an asymptomatic manner. due to physiological perturbation or upon immuno-c ... | 2016 | 27709564 |
| intestinal parasitic infections and associated epidemiological drivers in two rural communities of the bolivian chaco. | in 2013 a coproparasitological survey was carried out in two rural communities of the bolivian chaco to determine the prevalence of intestinal parasitic infections (ipis) and to investigate on possible infection drivers through a questionnaire interview. | 2016 | 27694735 |
| structures of parasite calreticulins provide insights into their flexibility and dual carbohydrate/peptide-binding properties. | calreticulin (crt) is a multifaceted protein, initially discovered as an endoplasmic reticulum (er) chaperone protein, that is essential in calcium metabolism. various implications in cancer, early development and immunology have been discovered more recently for crt, as well as its role as a dominant 'eat-me' prophagocytic signal. intriguingly, cell-surface exposure/secretion of crt is among the infective strategies used by parasites such as trypanosoma cruzi, entamoeba histolytica, taenia soli ... | 2016 | 27840680 |
| proteome analysis of excretory-secretory proteins of entamoeba histolytica hm1:imss via lc-esi-ms/ms and lc-maldi-tof/tof. | excretory-secretory (es) proteins of e. histolytica are thought to play important roles in the host invasion, metabolism, and defence. elucidation of the types and functions of e. histolytica es proteins can further our understanding of the disease pathogenesis. thus, the aim of this study is to use proteomics approach to better understand the complex es proteins of the protozoa. | 2016 | 27895543 |
| recent advances in entamoeba biology: rna interference, drug discovery, and gut microbiome. | in recent years, substantial progress has been made in understanding the molecular and cell biology of the human parasite entamoeba histolytica, an important pathogen with significant global impact. this review outlines some recent advances in the entamoeba field in the last five years, focusing on areas that have not recently been discussed in detail: (i) molecular mechanisms regulating parasite gene expression, (ii) new efforts at drug discovery using high-throughput drug screens, and (iii) th ... | 2016 | 27853522 |
| amoebic liver abscess: an unusual cause for a right iliac fossa mass: a case report. | amoebic liver abscess is the most common extra intestinal manifestation of amoebiasis in tropical countries. it usually presents with right hypochondrial pain, fever and anorexia. amoebic liver abscess has gained clinical significance due to the wide variety of clinical presentations which can cause diagnostic dilemmas and high mortality in untreated cases. | 2016 | 27931200 |
| phosphorylation of eukaryotic initiation factor-2α during stress and encystation in entamoeba species. | entamoeba histolytica is an enteric pathogen responsible for amoebic dysentery and liver abscess. it alternates between the host-restricted trophozoite form and the infective environmentally-stable cyst stage. throughout its lifecycle e. histolytica experiences stress, in part, from host immune pressure. conversion to cysts is presumed to be a stress-response. in other systems, stress induces phosphorylation of a serine residue on eukaryotic translation initiation factor-2α (eif2α). this inhibit ... | 2016 | 27930733 |
| metal dependence and branched rna cocrystal structures of the rna lariat debranching enzyme dbr1. | intron lariats are circular, branched rnas (brnas) produced during pre-mrna splicing. their unusual chemical and topological properties arise from branch-point nucleotides harboring vicinal 2',5'- and 3',5'-phosphodiester linkages. the 2',5'-bonds must be hydrolyzed by the rna debranching enzyme dbr1 before spliced introns can be degraded or processed into small nucleolar rna and microrna derived from intronic rna. here, we measure the activity of dbr1 from entamoeba histolytica by using a synth ... | 2016 | 27930312 |
| effect of mass stool examination and mass treatment for decreasing intestinal helminth and protozoan infection rates in bolivian children: a cross-sectional study. | bolivia is one of the countries with a high intestinal helminth and protozoan infection rate. despite the high prevalence of the parasitic infection, nationwide preventive measures for bolivian children have not yet been implemented. we evaluated the effect of mass stool examination and treatment as a strategy for decreasing the infection rate. this study was conducted between 2013 and 2015 in children aged 2-18 years. a total of 2,033 stool samples (575 in 2013, 815 in 2014 and 642 in 2015) wer ... | 2016 | 27923058 |
| amoeboma in a saudi resident: a case report. | amoebiasis is the third most frequent cause of mortality after malaria and schistosomiasis. in developed countries, amebiasis is also seen in migrants who have travelled to endemic areas. the factors responsible for its progression from intestinal amebiasis to an amebic liver abscess are not fully understood. | 2016 | 28348756 |
| clinical study on the efficacy of amoebex (coded herbal drug) compared with metronidazole for the treatment of amoebic dysentery. | amoebiasisis an infectious disease, which originated with the single-celled parasitic protozoan entamoeba histolytica. the parasitic amoeba infects the liver and intestine and may cause mild diarrhea and serious dysentery with bloody and mucoid stool. a study was conducted to evaluate the efficacy of amoebex (400mg), a herbal formulation for the treatment of amoebiasis infections as compared to that of metronidazole (400mg). the therapeutic evaluations of these medicines were carried out on 184 ... | 2016 | 28375117 |
| a study on hla-dr/dq typing in adult malay patients with acute amoebic liver abscess. | amoebiasis is a parasitic disease caused by entamoeba histolytica that may lead to death in developing countries. few important risk factors have been identified in the development of amoebic liver abscess (ala). there are limited reports that suggest an association between antigens of the major histocompatibility complex (mhc) particularly class ii antigens and ala development. this present work aimed at studying the possible association of hla antigens with ala and disease severity. results of ... | 2016 | 28087959 |
| analysis of the bacterial diversity in liver abscess: differences between pyogenic and amebic abscesses. | several recent studies have demonstrated that virulence in entamoeba histolytica is triggered in the presence of both pathogenic and nonpathogenic bacteria species using in vitro and in vivo experimental animal models. in this study, we examined samples aspirated from abscess material obtained from patients who were clinically diagnosed with amebic liver abscess (ala) or pyogenic liver abscess (pla). to determine the diversity of bacterial species in the abscesses, we performed partial 16s rrna ... | 2016 | 26572872 |
| prevalence of amoebiasis in a model research community and its confirmation using stool antigen elisa for entamoeba histolytica. | entamoeba histolytica (e. histolytica) produces an invasive disease called amoebiasis, which commonly produces diarrhea with or without blood in both children and adults, leading to high morbidity and mortality. entamoeba dispar (e. dispar) is a non invasive, non pathogenic organism. both entamoeba histolytica and entamoeba dispar look alike on microscopy and therefore cannot be differentiated unless checked on elisa, pcr or other specific method. to calculate the actual prevalence of pathogenic ... | 2016 | 27731816 |
| entamoeba histolytica and entamoeba dispar infection in mexican school children: genotyping and phylogenetic relationship. | this study aimed to determine the frequency of entamoeba histolytica and entamoeba dispar infection in school children in the community of tlaltizapan, in order to understand the dynamics of infection within the school and family spheres of this population. amoebiasis is an unsolved public health problem and an endemic disease in mexico. the incidence rate varies depending on the state; the most affected states show the highest numbers of new cases of amoebiasis per year. previously, we reported ... | 2016 | 27618874 |
| comparison of various methods in the diagnosis of entamoeba histolytica in stool and serum specimens. | entamoeba histolytica is indistinguishable from entamoeba dispar in direct microscopic examination. a definitive diagnosis of e. histolytica is important in terms of the treatment of the patient and to avoid unnecessary costs. this study's aim is to determine the prevalence of e. histolytica and to make a comparison of the different diagnostic tests in the patients specimens defined as e. histolytica/e. dispar infection. | 2016 | 27551176 |
| molecular detection of common intestinal parasites: a performance evaluation of the bd max™ enteric parasite panel. | the purpose of this study was to evaluate the level of agreement of the bd max™ enteric parasite panel (epp) with microscopy for the detection of giardia duodenalis, cryptosporidium spp. and entamoeba histolytica in stool samples. a total of 372 stool samples (partly collected on the basis of positive microscopy and partly unselected, consecutive sample submitted for parasite investigation) were tested with epp according to manufacturer's instructions and also using microscopy according to stand ... | 2016 | 27394158 |
| evaluation of five commercial methods for the extraction and purification of dna from human faecal samples for downstream molecular detection of the enteric protozoan parasites cryptosporidium spp., giardia duodenalis, and entamoeba spp. | high quality, pure dna is required for ensuring reliable and reproducible results in molecular diagnosis applications. a number of in-house and commercial methods are available for the extraction and purification of genomic dna from faecal material, each one offering a specific combination of performance, cost-effectiveness, and easiness of use that should be conveniently evaluated in function of the pathogen of interest. in this comparative study the marketed kits qiaamp dna stool mini (qiagen) ... | 2016 | 27241828 |
| frequency and molecular characterisation of entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, and entamoeba hartmanni in the context of water scarcity in northeastern brazil. | this study aimed to estimate the frequency, associated factors, and molecular characterisation of entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, andentamoeba hartmanni infections. we performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. faecal samples were processed through flotation and centrifugation methods.e. histolytica, e. dispar, e. moshkovskii, and e. hartmanni were identified by nested-polymerase chain reaction (pcr). th ... | 2016 | 26841049 |
| comparative analysis of genotypic diversity in entamoeba nuttalli isolates from tibetan macaques and rhesus macaques in china. | we have recently demonstrated the potentially virulent species entamoeba nuttalli as one of the highly prevalent parasites in rhesus macaques (macaca mulatta) in mount long-hu and gui-yang in china. tibetan macaque (macaca thibetana) is a unique species living in china. to evaluate the prevalence of entamoeba species in wild tibetan macaques, we obtained 89 stool samples in mount e-mei of si-chuan province in china. pcr analysis detected e. nuttalli, entamoeba coli, and entamoeba polecki st2 in ... | 2016 | 26723919 |
| prevalence of intestinal protozoans among schoolchildren in suburban areas near yangon, myanmar. | although intestinal protozoans are common etiologies of diarrhea, few studies have been conducted in myanmar. this study planned to investigate the prevalence of giardia lamblia, entamoeba coli, entamoeba histolytica, and endolimax nana among schoolchildren and their guardians in suburban areas near yangon, myanmar. we performed a cross-sectional survey among schoolchildren and their guardians from 7 primary schools in south dagon and hlaing thar yar districts, yangon, myanmar. stool samples wer ... | 2016 | 27417092 |
| molecular identification of entamoeba species in savanna woodland chimpanzees (pan troglodytes schweinfurthii). | to address the molecular diversity and occurrence of pathogenic species of the genus entamoeba spp. in wild non-human primates (nhp) we conducted molecular-phylogenetic analyses on entamoeba from wild chimpanzees living in the issa valley, tanzania. we compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (pcr)] and coproscopic detection (merthiolate-iodine-formaldehyde concentration) of entamoeba spp. we identified entamoeba spp. in 72 chimpanzee fecal samples ... | 2016 | 26935395 |
| high occurrence of blastocystis sp. subtypes 1-3 and giardia intestinalis assemblage b among patients in zanzibar, tanzania. | blastocystis is a common intestinal parasite with worldwide distribution but the distribution of blastocystis and its subtypes in east africa is largely unknown. in this study, we investigate the distribution of blastocystis subtypes in zanzibar, tanzania and report the prevalence of intestinal parasites using both molecular methods and microscopy. | 2016 | 27356981 |
| prevalence of dientamoeba fragilis, giardia duodenalis, entamoeba histolytica/dispar, and cryptosporidium spp in da nang, vietnam, detected by a multiplex real-time pcr. | we surveyed the prevalence of dientamoeba fragilis, giardia duodenalis, entamoeba histolytica, entamoeba dispar, and cryptosporidium spp in individuals with and without gastrointestinal (gi) symptoms residing in and around da nang city, vietnam. fecal samples were collected from children (n = 100) and adults (n = 80) with gi symptoms and from healthy individuals (n = 88) reporting no gi symptoms. parasite detection was performed by multiplex real-time pcr. overall, except for g. duodenalis, we f ... | 2016 | 27102222 |
| drug repurposing: mining protozoan proteomes for targets of known bioactive compounds. | to identify potential opportunities for drug repurposing by developing an automated approach to pre-screen the predicted proteomes of any organism against databases of known drug targets using only freely available resources. | 2016 | 23757409 |
| multicenter evaluation of bd max enteric parasite real-time pcr assay for detection of giardia duodenalis, cryptosporidium hominis, cryptosporidium parvum, and entamoeba histolytica. | common causes of chronic diarrhea among travelers worldwide include protozoan parasites. the majority of parasitic infections are caused by giardia duodenalis, entamoeba histolytica, cryptosporidium parvum, and cryptosporidium hominis similarly, these species cause the majority of parasitic diarrhea acquired in the united states. detection of parasites by gold standard microscopic methods is time-consuming and requires considerable expertise; enzyme immunoassays and direct fluorescent-antibody ( ... | 2016 | 27535690 |
| is real-time pcr-based diagnosis similar in performance to routine parasitological examination for the identification of giardia intestinalis, cryptosporidium parvum/cryptosporidium hominis and entamoeba histolytica from stool samples? evaluation of a new commercial multiplex pcr assay and literature review. | microscopy is the reference standard for routine laboratory diagnosis in faecal parasitology but there is growing interest in alternative methods to overcome the limitations of microscopic examination, which is time-consuming and highly dependent on an operator's skills and expertise. compared with microscopy, dna detection by pcr is simple and can offer a better turnaround time. however, pcr performances remain difficult to assess as most studies have been conducted on a limited number of posit ... | 2016 | 26548509 |
| prevalence of intestinal parasites in referred individuals to the medical centers of tonekabon city, mazandaran province. | the aim of the present study was to determine the prevalence of intestinal parasites and their relation with socio-demographic data in referred individuals to the medical centers in tonekabon, mazandaran province, 2015. | 2016 | 28224032 |
| cryptosporidium parvum among coprolites from la cueva de los muertos chiquitos (600-800 ce), rio zape valley, durango, mexico. | : in the present study, 90 coprolites from la cueva de los muertos chiquitos (cmc) were subjected to enzyme-linked immunosorbent assay (elisa) tests for 3 diarrhea-inducing protozoan parasites, entamoeba histolytica , giardia duodenalis , and cryptosporidium parvum , to determine whether these parasites were present among the people who utilized this cave 1,200-1,400 yr ago. these people, the loma san gabriel, developed as a culture out of the archaic los caracoles population and lived througho ... | 2016 | 27098916 |
| structure of methionine γ-lyase from clostridium sporogenes. | methionine γ-lyase (mgl) is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the γ-elimination reaction of l-methionine. the enzyme is a promising target for therapeutic intervention in some anaerobic pathogens and has attracted interest as a potential cancer treatment. the crystal structure of mgl from clostridium sporogenes has been determined at 2.37 å resolution. the fold of the protein is similar to those of homologous enzymes from citrobacter freundii, entamoeba histolytica, pseudo ... | 2016 | 26750487 |
| nitroimidazole carboxamides as antiparasitic agents targeting giardia lamblia, entamoeba histolytica and trichomonas vaginalis. | diarrhoeal diseases caused by the intestinal parasites giardia lamblia and entamoeba histolytica constitute a major global health burden. nitroimidazoles are first-line drugs for the treatment of giardiasis and amebiasis, with metronidazole 1 being the most commonly used drug worldwide. however, treatment failures in giardiasis occur in up to 20% of cases and development of resistance to metronidazole is of concern. we have re-examined 'old' nitroimidazoles as a foundation for the systematic dev ... | 2016 | 27236016 |
| study of the gastrointestinal parasitic fauna of captive non-human primates (macaca fascicularis). | the aim of this study was to examine helminths and protozoans in cynomolgus macaques (macaca fascicularis) imported from registered breeding facilities in china and their relation to health risks for non-human primate handlers in biomedical research centers and in breeding facilities. fresh fecal samples were collected from a total of 443 m. fascicularis and analyzed by copromicroscopical analysis, immunoenzymatic, or molecular assays. as to helminths, whose eggs were shed in 2.03% of the sample ... | 2016 | 26374536 |
| effect of sanitation and water treatment on intestinal protozoa infection: a systematic review and meta-analysis. | pathogenic intestinal protozoa infections are responsible for substantial mortality and morbidity, particularly in settings where people lack improved sanitation and safe drinking water. we assessed the relation between access to, and use of, sanitation facilities and water treatment and infection with intestinal protozoa. | 2016 | 26404667 |
| distribution of parasites detected in stool samples of patients admitted to our parasitology laboratory during a three-year period between 2012 and 2014. | parasitic diseases are among the major public health issues worldwide. a number of tests are available for diagnosis, but the sentivity and specifity of these tests are assumed to be insufficient. nevertheless, the most common diagnostic method is microscopic examination. in this study, we aimed to introduce the distribution of parasites detected in stool samples of patients admitted to our laboratory on the basis of parameters such as, age, and gender during a 3-year period between 2012 and 201 ... | 2016 | 27905282 |
| prevalence and risk factors of intestinal protozoan infections: a population-based study in rural areas of boyer-ahmad district, southwestern iran. | parasitic infections are still a significant health problem in rural areas in developing countries including iran. there is no recent population-based data about the prevalence of human intestinal parasites in most rural areas of iran. the current study aimed to determine the prevalence of intestinal protozoan infection in inhabitants of rural areas of boyer-ahmad district, southwestern iran. | 2016 | 27884121 |
| multiplex real-time pcr assay targeting eight parasites customized to the korean population: potential use for detection in diarrheal stool samples from gastroenteritis patients. | intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. we developed a multiplex real-time pcr assay for detection of eight intestinal parasites and prospectively evaluated it for patients with gastroenteritis. the assay targeted cryptosporidium parvum, giardia lamblia, entamoeba histolytica, blastocystis hominis, dientamoeba fragilis, clonorchis sinensis, metagonimus yokogawai, an ... | 2016 | 27861635 |
| a decade of intestinal protozoan epidemiology among settled immigrants in qatar. | the world health organization estimates that about 3.5 billion people worldwide are affected by intestinal parasitic infections. reports have already emphasized the role of immigrants in outbreaks of parasitic diseases in industrialized countries. with the mass influx of immigrants to qatar, patent intestinal parasitic infections have been observed. herein, the prevalence of intestinal protozoan infections was analysed in 29,286 records of subjects referred for stool examination at the hamad med ... | 2016 | 27496143 |
| irritable bowel syndrome and intestinal parasites: a view from south america. | irritable bowel syndrome (ibs) is a functional gastrointestinal disorder of uncertain etiology. several studies have proposed the possible role of intestinal parasites in the pathogenesis of ibs. we aimed to summarize the epidemiological studies that describe a possible link between intestinal parasites and ibs, with special interest in endemic areas for intestinal parasitism such as south america. a comprehensive review of the literature was conducted by using the keywords: irritable bowel synd ... | 2016 | 27409092 |
| neglected waterborne parasitic protozoa and their detection in water. | outbreak incidents raise the question of whether the less frequent aetiological agents of outbreaks are really less frequent in water. alternatively, waterborne transmission could be relevant, but the lack of attention and rapid, sensitive methods to recover and detect the exogenous stages in water may keep them under-recognized. high quality information on the prevalence and detection of less frequent waterborne protozoa, such as cyclospora cayetanensis, toxoplasma gondii, isospora belli, balan ... | 2016 | 27281375 |
| infectious diseases in immigrant population related to the time of residence in spain. | the aim of this study was to evaluate the data on the main imported infectious diseases and public health issues arising from the risk of transmission of tropical and common diseases in the immigrant population. during the period of study, 2,426 immigrants were attended in the tropical medicine unit of the hospital of poniente. for each patient, a complete screening for common and tropical diseases was performed. the prevalence and main features of intestinal and urinary parasites, microfilarias ... | 2016 | 25466580 |
| children with moderate-high infection with entamoeba coli have higher percentage of body and abdominal fat than non-infected children. | intestinal parasites, virus and bacterial infections are positively associated with obesity and adiposity in vitro and in animal models, but conclusive evidence of this relationship in humans is lacking. the aim of this cross-sectional study was to determine differences in adiposity between infected and non-infected children, with a high prevalence of intestinal parasitic infection and obesity. | 2016 | 26663860 |
| risk factors associated with parasitic infection among municipality solid-waste workers in an egyptian community. | solid-waste management is associated with several health hazards, particularly parasitic infection. the objective of the study was to determine the association between risk factors and the occurrence of intestinal parasitic infections (potentially pathogenic) among municipal waste collectors in alexandria, egypt. a cross-sectional survey was conducted in the main municipality company in alexandria. a total of 346 municipality solid-waste workers (mswws) was interviewed using an in-depth question ... | 2016 | 26606452 |
| prevalence of intestinal parasitic infections and associated risk factors among pregnant women attending antenatal care center at felege hiwot referral hospital, northwest ethiopia. | parasitic infections affect tens of millions of pregnant women worldwide, and directly or indirectly lead to a spectrum of adverse maternal and fetal/placental effects. the objective of this study was to assess the prevalence of intestinal parasite infections and associated risk factors among pregnant women attending antenatal care center in felege hiwot referral hospital, bahir dar city, northwest ethiopia. | 2016 | 27716099 |
| parasitic contamination of fresh vegetables sold at central markets in khartoum state, sudan. | fresh vegetables are considered as vital nutrients of a healthy diet as they supply the body with essential supplements. the consumption of raw vegetables is the main way for transmission of intestinal parasitic organisms. this study was aimed at detecting the parasitic contamination in fresh vegetables sold in two central open-aired markets in khartoum state, sudan. | 2016 | 26968696 |
| surveillance for parasites in unaccompanied minor refugees migrating to germany in 2015. | in 2015, most of the refugees arriving in germany originated from countries with poor hygienic and sanitary conditions. stool samples of 1,230 minor refugees unaccompanied by adults were investigated for possible parasites. giardia lamblia was by far the most frequently detected parasite (n=165); all other parasites were considerably less frequent and encountered in the following order: hymenolepis nana (n=23), entamoeba histolytica (n=17), trichuris trichiura (n=8), and blastocystis hominis (n= ... | 2016 | 26958459 |
| risk of intestinal parasitic infections in people with different exposures to wastewater and fecal sludge in kampala, uganda: a cross-sectional study. | there are health risks associated with wastewater and fecal sludge management and use, but little is known about the magnitude, particularly in rapidly growing urban settings of low- and middle-income countries. we assessed the point-prevalence and risk factors of intestinal parasite infections in people with different exposures to wastewater and fecal sludge in kampala, uganda. | 2016 | 26938060 |
| marked seasonality of cyclospora cayetanensis infections: ten-year observation of hospital cases, honduras. | document seasonality occurrence and epidemiologic characteristics of cyclospora cayetanensis infections during a 10-year period from patients consulting at the university hospital, honduras. | 2016 | 26847438 |
| prevalence and factors associated with intestinal parasitic infections among food handlers of southern ethiopia: cross sectional study. | globally about one third of the total population is estimated to be infected with intestinal parasites, of which, the majority are people living in tropical and sub-tropical parts of the world. cases of intestinal parasitosis are also highly abundant in ethiopia and hence the aim of present study was to assess prevalence and predictors of intestinal parasitic infections among food handlers working in arba minch university students' cafeteria, south ethiopia. | 2016 | 26831331 |
| household and personal factors are sources of heterogenity in intestinal parasite clearance among mexican children 6-15 months of age supplemented with vitamin a and zinc. | a randomised, double-blind, placebo-controlled trial was carried out among mexico children aged 6-15 months to determine how household characteristics modify vitamin a and zinc supplementation efficacy on ascaris lumbricoides, giardia intestinalis and entamoeba histolytica/e. dispar infection durations. children assigned to receive vitamin a every 2 months, a daily zinc supplement, a combined vitamin a-zinc supplement or a placebo were followed for 1 year. parametric hazard models were fit to in ... | 2016 | 26772449 |
| etiology of diarrhea in children younger than 5 years attending the bengo general hospital in angola. | diarrheal disease is among the leading causes of death in children younger than 5 years, especially in developing countries. the aim of this study was to investigate the most frequent etiological agents of diarrhea and its associated factors in children younger than 5 years attending the bengo general hospital in angola. | 2016 | 26761347 |
| migrant workers in malaysia: current implications of sociodemographic and environmental characteristics in the transmission of intestinal parasitic infections. | a cross-sectional study of intestinal parasitic infections amongst migrant workers in malaysia was conducted. a total of 388 workers were recruited from five sectors including manufacturing, construction, plantation, domestic and food services. the majority were recruited from indonesia (n = 167, 43.3%), followed by nepal (n = 81, 20.9%), bangladesh (n = 70, 18%), india (n = 47, 12.1%) and myanmar (n = 23, 5.9.2%). a total of four nematode species (ascaris lumbricoides, trichuris trichiura, ente ... | 2016 | 27806046 |
| prevalence of intestinal parasite infections among patients in local public hospitals of hail, northwestern saudi arabia. | to evaluate the prevalence of intestinal parasites among patients in hail, northwestern saudi arabia. | 2016 | 26851785 |
| multi-parallel qpcr provides increased sensitivity and diagnostic breadth for gastrointestinal parasites of humans: field-based inferences on the impact of mass deworming. | although chronic morbidity in humans from soil transmitted helminth (sth) infections can be reduced by anthelmintic treatment, inconsistent diagnostic tools make it difficult to reliably measure the impact of deworming programs and often miss light helminth infections. | 2016 | 26813411 |
| the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china. | acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ... | 2016 | 27965649 |
| a study of parasitic and bacterial pathogens associated with diarrhea in hiv-positive patients. | diarrhea is a common complication of acquired immune deficiency syndrome (aids), occurring in almost 90% of aids patients in developing countries like india. the present study was aimed to determine the prevalence and microbiological profile of pathogens associated with diarrhea in human immunodeficiency virus (hiv) positive patients and their relation to cd4 counts. | 2016 | 27795876 |
| evaluation of the nanochip® gastrointestinal panel (gip) test for simultaneous detection of parasitic and bacterial enteric pathogens in fecal specimens. | infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. current laboratory stool testing has a long turnaround time (tat) and demands highly qualified personnel and multiple techniques. the need for high throughput and the number of possible enteric pathogens compels the implementation of a molecular approach which uses multiplex technology, without compromising pe ... | 2016 | 27447173 |
| entamoeba histolytica trophozoites and lipopeptidophosphoglycan trigger human neutrophil extracellular traps. | neutrophil defense mechanisms include phagocytosis, degranulation and the formation of extracellular traps (net). these networks of dna are triggered by several immune and microbial factors, representing a defense strategy to prevent microbial spread by trapping/killing pathogens. this may be important against entamoeba histolytica, since its large size hinders its phagocytosis. the aim of this study was to determine whether e. histolytica and their lipopeptidophosphoglycan (ehlppg) induce the f ... | 2016 | 27415627 |
| comparison of nested-multiplex, taqman & sybr green real-time pcr in diagnosis of amoebic liver abscess in a tertiary health care institute in india. | amoebiasis is a common parasitic infection caused by entamoeba histolytica and amoebic liver abscess (ala) is the most common extraintestinal manifestation of amoebiasis. the aim of this study was to standardise real-time pcr assays (taqman and sybr green) to detect e. histolytica from liver abscess pus and stool samples and compare its results with nested-multiplex pcr. | 2016 | 26997014 |
| identification of repressive and active epigenetic marks and nuclear bodies in entamoeba histolytica. | in human hosts, entamoeba histolytica cysts can develop into trophozoites, suggesting that the life cycle of this parasite are regulated by changes in gene expression. to date, some evidence has suggested that epigenetic mechanisms such as dna methylation and histone modification are involved in the regulation of gene expression in entamoeba. some post-translational modifications (ptms) at the n-terminus of e. histolytica's histones have been reported experimentally, including tri-methylation in ... | 2016 | 26767976 |
| ehnpc1 and ehnpc2 proteins participate in trafficking of exogenous cholesterol in entamoeba histolytica trophozoites: relevance for phagocytosis. | entamoeba histolytica, the highly phagocytic protozoan causative of human amoebiasis lacks the machinery to synthesize cholesterol. here, we investigated the presence of npc1 and npc2 proteins in this parasite, which are involved in cholesterol trafficking in mammals. bioinformatics analysis revealed one ehnpc1 and two ehnpc2 genes. ehnpc1 appeared as a transmembrane protein and both ehnpc2 as peripheral membrane proteins. molecular docking predicted that ehnpc1 and ehnpc2 bind cholesterol and i ... | 2016 | 28002502 |
| discordances between serology and culture for strongyloides in an ethiopian adopted child with multiple parasitic infections: a case report. | infectious diseases screening of international adoptees is complex because of the concurrence of different pathogens in a child at same time. we describe an international adopted child born at ethiopia infected by 5 different pathogens (hymenolepis nana, giardia intestinalis, entamoeba histolytica, strongyloides stercoralis, and trichuris trichiura), 2 of them s. stercoralis and e. histolytica with a capacity to develop severe clinical complications if not detected promptly with appropriate diag ... | 2016 | 26962825 |
| discovery of ppi-type phosphoenolpyruvate carboxykinase genes in eukaryotes and bacteria. | phosphoenolpyruvate carboxykinase (pepck) is one of the pivotal enzymes that regulates the carbon flow of the central metabolism by fixing co2 to phosphoenolpyruvate (pep) to produce oxaloacetate or vice versa. whereas atp- and gtp-type pepcks have been well studied, and their protein identities are established, inorganic pyrophosphate (ppi)-type pepck (ppi-pepck) is poorly characterized. despite extensive enzymological studies, its protein identity and encoding gene remain unknown. in this stud ... | 2015 | 26269598 |
| the nlrp3 inflammasome is a pathogen sensor for invasive entamoeba histolytica via activation of α5β1 integrin at the macrophage-amebae intercellular junction. | entamoeba histolytica (eh) is an extracellular protozoan parasite of humans that invades the colon to cause life-threatening intestinal and extra-intestinal amebiasis. colonized eh is asymptomatic, however, when trophozoites adhere to host cells there is a considerable inflammatory response that is critical in the pathogenesis of amebiasis. the host and/or parasite factors that trigger the inflammatory response to invading eh are not well understood. we recently identified that eh adherence to m ... | 2015 | 25955828 |
| prevalence of anal human papillomavirus infection and risk factors among hiv-positive patients in tokyo, japan. | oncogenic human papillomavirus (hpv) infection, particularly multiple hpv types, is recognized as a necessary cause of anal cancer. however, a limited number of studies have reported the prevalence of anal hpv infection in asia. we determined the prevalence, genotypes, and risk factors for anal hpv infection in japanese hiv-positive men who have sex with men (msm), heterosexual men, and women. | 2015 | 26368294 |
| structure of uridine diphosphate n-acetylglucosamine pyrophosphorylase from entamoeba histolytica. | uridine diphosphate n-acetylglucosamine pyrophosphorylase (uap) catalyzes the final step in the synthesis of udp-glcnac, which is involved in cell-wall biogenesis in plants and fungi and in protein glycosylation. small-molecule inhibitors have been developed against uap from trypanosoma brucei that target an allosteric pocket to provide selectivity over the human enzyme. a 1.8 å resolution crystal structure was determined of uap from entamoeba histolytica, an anaerobic parasitic protozoan that c ... | 2015 | 25945709 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| serological investigation to identify risk factors for post-flood infectious diseases: a longitudinal survey among people displaced by typhoon morakot in taiwan. | after typhoon morakot struck taiwan in 2009, thousands of taiwanese citizens were displaced to shelters for several weeks. others were placed in urban communities where they had family members. this study aimed to investigate serological status in both groups and identify risk factors associated with seroconversion of infectious diseases. | 2015 | 25976763 |
| molecular and biochemical characterization of entamoeba histolytica fructokinase. | entamoeba histolytica is the causative agent of amoebic dysentery and liver abscess. the medium for its axenic culture contains glucose as energy source, and we addressed the question whether e. histolytica can also use fructose instead. as the amoebic hexokinases do not phosphorylate fructose, a separate fructokinase is essential. the genome project revealed a single candidate gene encoding an e. histolytica homolog of bacterial fructokinases. this gene was cloned, and the recombinant enzyme ha ... | 2015 | 25700717 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| phenotypic and transcriptional profiling in entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance. | antimicrobial chemotherapy is critical in the fight against infectious diseases caused by entamoeba histolytica. among the drugs available for the treatment of amebiasis, metronidazole (mtz) is considered the drug of choice. recently, in vitro studies have described mtz resistance and the potential mechanisms involved. costs to fitness and adaptive responses associated with resistance, however, have not been investigated. in this study we generated an hm-1 derived strain resistant to 12 μm mtz ( ... | 2015 | 25999919 |
| identification of human intestinal parasites affecting an asymptomatic peri-urban argentinian population using multi-parallel quantitative real-time polymerase chain reaction. | in resource-limited countries, stool microscopy is the diagnostic test of choice for intestinal parasites (soil-transmitted helminths and/or intestinal protozoa). however, sensitivity and specificity is low. improved diagnosis of intestinal parasites is especially important for accurate measurements of prevalence and intensity of infections in endemic areas. | 2015 | 26183074 |
| anaerobic respiration: in vitro efficacy of nitazoxanide against mitochondriate acanthamoeba castellanii of the t4 genotype. | acanthamoeba is an opportunistic protist pathogen that is responsible for serious human and animal infection. being one of the most frequently isolated protists from the environment, it is likely that it readily encounters microaerophilic environments. for respiration under anaerobic or low oxygen conditions in several amitochondriate protists, decarboxylation of pyruvate is catalyzed by pyruvate ferredoxin oxidoreductase instead of pyruvate dehydrogenase. in support, nitazoxanide, an inhibitor ... | 2015 | 26297676 |
| a comparative study of bacterial and parasitic intestinal infections in india. | infectious diarrhea causes a major health problem in developing countries with significant morbidity and mortality. very often, rehydration therapy alone does not suffice, mandating the use of antimicrobial agents. however, rapidly decreasing antimicrobial susceptibility is complicating the matters. | 2015 | 25954615 |
| coinfection with malaria and intestinal parasites, and its association with anaemia in children in cameroon. | the purpose of this study was to determine the prevalence of coinfection with malaria and intestinal parasites, as well as to determine its association with anaemia in children aged 10 years and below in muyuka, cameroon. | 2015 | 26445484 |
| infection by intestinal parasites, stunting and anemia in school-aged children from southern angola. | intestinal parasites are responsible for morbidity in children worldwide, especially in low income countries. in the present study we determine the prevalence of intestinal parasites and explore its association with anemia and stunting in school-aged children. | 2015 | 26371758 |
| pervasiveness of intestinal protozoan and worm incursion in idp's (north waziristan agency, kpk-pakistan) children of 6-16 years. | to evaluate the prevalence of intestinal protozoan and worm infestation in children of families having migrated due to military operation. | 2015 | 26338737 |
| heavy burden of intestinal parasite infections in kalena rongo village, a rural area in south west sumba, eastern part of indonesia: a cross sectional study. | intestinal parasitic infections (ipis) are one of the major public health problems, especially in the rural area of developing countries with low socio-economic status and poor sanitation. the study was aimed to determine the prevalence of ipis among the inhabitants of a rural area in south west sumba, eastern part of indonesia. | 2015 | 26702820 |