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effect of silage from ryegrass intercropped with winter or common vetch for grazing dairy cows in small-scale dairy systems in mexico.the objective was to determine the effect of including silages of annual ryegrass (lolium multiflorum) intercropped with winter vetch (vicia villosa) (arg-vv) or with common vetch (vicia sativa) (arg-vs) compared with maize silage (ms) on milk yield and milk composition of dairy cows grazing cultivated perennial ryegrass-white clover pastures with supplemented concentrate during the dry season. six holstein dairy cows with a mean yield of 19.0 kg/cow/day at the beginning of the experiment were r ...201121327716
plants growing on contaminated and brownfield sites appropriate for use in organisation for economic co-operation and development terrestrial plant growth test.the organisation for economic co-operation and development (oecd) terrestrial plant test is often used for the ecological risk assessment of contaminated land. however, its origins in plant protection product testing mean that the species recommended in the oecd guidelines are unlikely to occur on contaminated land. six alternative species were tested on contaminated soils from a former zn smelter and a metal fragmentizer with elevated concentrations of cd, cu, pb, and zn. the response of the al ...201120853450
characterization of chloroplast dna microsatellites from saccharum spp and related species.microsatellites, or simple sequence repeats (ssrs), and their flanking regions in chloroplast genomes (plastomes) of some species of the family poaceae were analyzed in silico to look for dna sequence variations. comparison of the complete chloroplast dna sequences (cpdnas) of sugarcane (saccharum hybrid cv. sp-80-3280 and s. officinarum cv. nco310) and related species, agrostis stolonifera, brachypodium distachyon, hordeum vulgare subsp vulgare, lolium perenne, oryza nivara, o. sativa sub ...201121948764
comparative evaluation of laboratory-scale silages using standard glass jar silages or vacuum-packed model silages.the objective of this study was to compare the fermentation variables of laboratory-scale silages made in glass preserving jars (glass) and vacuum-packed plastic bags (rostock model silages, romos). silages were prepared from perennial ryegrass (fresh and wilted, 151 g kg(-1) and 286 g kg(-1) dry matter (dm), respectively) and remoistened coarsely ground rye grain (650 g kg(-1) dm) either with or without the addition of a lactic acid bacteria inoculant (3+ù10(5) colony forming units (cfu) g(-1) ...201121384351
esi-mass spectrometric and hplc elucidation of a new ergot alkaloid from perennial ryegrass hay silage associated with bovine reproductive problems.this case report involves four dairies in the willamette valley, oregon, which experienced reproductive problems associated with the presence of a large, previously unidentified, peak eluting at 5 min in a standard ergovaline high-performance liquid chromatography assay of perennial ryegrass silage fed to those animals. mycotoxin analysis of the silage was negative, as was serological screening of the herds for infectious bovine rhinotracheitis, bovine diarrhea virus and leptospirosis, including ...201121506724
functional immunoglobulin e cross-reactivity between pas n 1 of bahia grass pollen and other group 1 grass pollen allergens.grass pollens are major triggers of allergic rhinitis and asthma, but the immunological relationships between pollen allergens of the subtropical bahia grass, paspalum notatum, and temperate grasses are unresolved.201121231976
energy efficiency and energy homeostasis as genetic and epigenetic components of plant performance and crop productivity.the importance of energy metabolism in plant performance and plant productivity is conceptually well recognized. in the eighties, several independent studies in lolium perenne (ryegrass), zea mays (maize), and festuca arundinacea (tall fescue) correlated low respiration rates with high yields. similar reports in the nineties largely confirmed this correlation in solanum lycopersicum (tomato) and cucumis sativus (cucumber). however, selection for reduced respiration does not always result in high ...201121411363
phytotoxicity and uptake of nitroglycerin in a natural sandy loam soil.nitroglycerin (ng) is widely used for the production of explosives and solid propellants, and is a soil contaminant of concern at some military training ranges. ng phytotoxicity data reported in the literature cannot be applied directly to development of ecotoxicological benchmarks for plant exposures in soil because they were determined in studies using hydroponic media, cell cultures, and transgenic plants. toxicities of ng in the present studies were evaluated for alfalfa (medicago sativa), b ...201121975007
allelopathic effect of bromus spp. and lolium spp. shoot extracts on some crops.allelopathy is an untapped resource for weed control in crops that could give good possibilities for environmentally sound, integrated crop production. allelopathy is defined as the direct or indirect harmful or beneficial effects of one plant on another through the production of chemical compounds, called allelochemicals, which escape into the environment. allelochemicals can be produced by weeds and affect crops, and the reverse is also true. allelopathic interactions include weed-weed, weed-c ...201122696963
toxicity of 2,4-dinitrotoluene to terrestrial plants in natural soils.the presence of energetic materials (used as explosives and propellants) at contaminated sites is a growing international issue, particularly with respect to military base closures and demilitarization policies. improved understanding of the ecotoxicological effects of these materials is needed in order to accurately assess the potential exposure risks and impacts on the environment and its ecosystems. we studied the toxicity of the nitroaromatic energetic material 2,4-dinitrotoluene (2,4-dnt) o ...201020471667
the complete chloroplast genome of tall fescue (lolium arundinaceum; poaceae) and comparison of whole plastomes from the family poaceae.in this paper, we describe the complete chloroplast genome of lolium arundinaceum. this sequence is the culmination of a long-term project completed by >400 undergraduates who took general genetics at middle tennessee state university from 2004-2007. it was undertaken in an attempt to introduce these students to an open-ended experiential/exploratory lesson to produce and analyze novel data. the data they produced should provide the necessary information for both phylogenetic comparisons and pla ...201021622366
the appreciation of mineral element accumulation level in some herbaceous plants species by icp-aes method.from the metallurgic industry zone of dambovita county, we harvested and analyzed seven herbaceous plants species (lolium perenne, festuca pratensis, stipa capillata, agrostis alba, cynodon dactylon, luzula campestris, and agrostis tenuis) to establish the heavy metal accumulation levels in these species.201020165925
uptake and transformation of soil [14c]-trinitrotoluene by cool-season grasses.this study investigated the fate and uptake of [(14)c]-tnt from soil into orchardgrass (dactylis glomerata), perennial ryegrass (lolium perenne), and tall fescue (festuca arundinacea) over a one year period in a greenhouse-controlled environment. pots (n = 4 for each grass, containing 10 mg cold tnt/kg soil + 1.2 mg [(14)c]-tnt/kg soil and controls with no tnt) were exposed to light and temperature conditions typical of june at 45 degrees n for 369 days. three plant harvests were made (63, 181, ...201020666491
predicting molybdenum toxicity to higher plants: estimation of toxicity threshold values.four plant species (oilseed rape, brassica napus l.; red clover, trifolium pratense l.; ryegrass, lolium perenne l.; and tomato, lycopersicon esculentum l.) were tested on ten soils varying widely in soil properties to assess molybdenum (mo) toxicity. a larger range (66-fold-609-fold) of added mo concentrations resulting in 50% inhibition of yield (ed50) was found among soils than among plant species (2-fold-38-fold), which illustrated that the soils differed widely in the expression of mo toxic ...201020656390
effect of amendments on phytoavailability and fractionation of copper and zinc in a contaminated soil.the ability of amendments to modify the soil properties and influence plants to immobilise cu and zn was studied in a naturally contaminated, additionally spiked podzolic soil. lolium perenne l (perennial rye grass), festuca rubra l (creeping red fescue) and poa pratensis l (kentucky blue grass) were tested in a pot study in the presence of soil amendments (lime, phosphate, and compost, individually and in combination) to assess the effect of soil-plant-amendment interaction on phytostabilisatio ...201021166277
comparison of planted soil infiltration systems for treatment of log yard runoff.treatment of log yard runoff is required to avoid contamination of receiving watercourses. the research aim was to assess if infiltration of log yard runoff through planted soil systems is successful and if different plant species affect the treatment performance at a field-scale experimental site in sweden (2005 to 2007). contaminated runoff from the log yard of a sawmill was infiltrated through soil planted with alnus glutinosa (l.) gärtner (common alder), salix schwerinii x viminalis (willow ...201020669729
platanus pollen allergen, pla a 1: quantification in the atmosphere and influence on a sensitizing population.the allergic response in susceptible patients does not always coincide with the presence and magnitude of airborne pollen counts. the prevalence of allergy to platanus is currently moderate, although the percentage of monosensitized patients is low. this hinders accurate interpretation of the relationship between the amount of pollen inhaled and the patient's symptoms.201020813013
which allergen extract for grass pollen immunotherapy? an in vitro study.grass pollen is a major cause of allergy throughout the world. the only treatment targeting the causes and not only the symptoms of allergy is specific immunotherapy (it). a number of controlled trials demonstrated the efficacy of it in grass pollen allergic subjects, most using extracts of multiple grasses but some using extracts of a single grass. the optimal grass extract for it has not yet been established.201020653430
transcriptional responses of italian ryegrass during interaction with xanthomonas translucens pv. graminis reveal novel candidate genes for bacterial wilt resistance.xanthomonas translucens pv. graminis (xtg) causes bacterial wilt, a severe disease of forage grasses such as italian ryegrass (lolium multiflorum lam.). in order to gain a more detailed understanding of the genetic control of resistance mechanisms and to provide prerequisites for marker assisted selection, the partial transcriptomes of two italian ryegrass genotypes, one resistant and one susceptible to bacterial wilt were compared at four time points after xtg infection. a cdna microarray devel ...201020976589
conservation characteristics of wilted perennial ryegrass silage made using biological or chemical additives.the effects of 7 additive treatments on the fermentation and aerobic stability characteristics of wilted grass silage were studied under laboratory conditions. treatments included no additive applied (untreated control), ammonium tetraformate at 3 and 6 l/t, homofermentative lactic acid bacteria alone ((ho)lab), a mixture of lactobacillus buchneri plus homofermentative lactic acid bacteria ((he+ho)lab), and an antimicrobial mixture of sodium benzoate, sodium propionate, sodium nitrite, and hexam ...201020105535
phytoavailability and fractionation of lead and manganese in a contaminated soil after application of three amendments.studies were conducted to determine the best management practice for immobilisation of toxic pb and mn in soil and the interaction of these metal contaminants with the associated plants. the research protocol comprises addition of soil amendments to accelerate physico-chemically driven sorption processes and growth of appropriate plant species to reduce physiologically driven uptake of pb and mn. lolium perenne l (perennial rye grass), festuca rubra l (creeping red fescue) and poa pratensis l (k ...201020219365
a comparison between holstein-friesian and jersey dairy cows and their f(1) hybrid on milk fatty acid composition under grazing conditions.the objective of this study was to investigate the effect of 2 breeds, holstein and jersey, and their f(1) hybrid (jersey x holstein) on milk fatty acid (fa) concentrations under grazing conditions, especially conjugated linoleic acid (cla) and n-3 polyunsaturated fatty acids because of their importance to human health. eighty-one cows (27 per breed grouping) were allocated a predominantly perennial ryegrass pasture. samples were collected over 2 periods (june and july). breed affected dry matte ...201020412933
identification of abc transporters from lolium perenne l. that are regulated by toxic levels of selenium.selenium is an essential micronutrient for animals and humans, but can be toxic at higher levels. manipulation of se metabolism in plants may enable plants to be tailored to enhance se content for human and animal consumption and to decontaminate se polluted soils. here, we generated subtracted cdna libraries from perennial ryegrass roots and leaves, enriched for genes which expression is enhanced under toxic levels of selenium. the libraries were sequenced using next generation sequencing techn ...201020063009
forage as a primary source of mycotoxins in animal diets.the issue of moulds and, thus, contamination with mycotoxins is very topical, particularly in connexion with forages from grass stands used at the end of the growing season. deoxynivalenol (don), zearalenone (zea), fumonisins (fum) and aflatoxins (afl) are among the most common mycotoxins. the aim of the paper was to determine concentrations of mycotoxins in selected grasses (lolium perenne, festulolium pabulare, festulolium braunii) and their mixtures with festuca rubra an/or poa pratensis duri ...201021318013
rhinitis is also common in infants with asthma.the aim of this study was to assess the frequency of rhinitis in asthmatic infants. a cross-sectional study was conducted using clinical data obtained from a standardized allergy work-up form that includes specific questions on common allergic diseases. asthmatic patients were seen at the first visit to the pediatric allergy unit, from january 2001 to january 2006, were selected for analysis. diagnosis of allergic rhinitis was based on the presence of two or more nasal symptoms (sneezing, itchin ...201020548130
bioassay and use in irrigation of untreated and treated wastewaters from phosphate fertilizer industry.wastewater from phosphate fertilizer industry that contains essentially a significant amount of both fluoride and phosphate was treated by separative precipitation of fluoride ions with hydrated lime. thus, a phosphate-rich effluent with low content of fluoride was obtained. the microtoxicity of the treated wastewater was then monitored by lumistox and its phytotoxicity was investigated on tomato (lycopersicon esculentum), wheat (triticum aestivum), maize (zea mays), ryegrass (lolium perenne), a ...201020061024
effects of maternal protein nutrition and subsequent grazing on chicory (cichorium intybus) on parasitism and performance of lambs.forty-eight 4- to 5-yr-old blackface x bluefaced leicester (mule) ewes and their 24-d-old twin lambs were used to assess the effects of maternal protein nutrition and subsequent grazing on chicory (cichorium intybus) on performance and parasitism. the experiment consisted of 2 grazing periods: safe pasture period and experimental pasture period. during an adaptation period of 66 d, ewes were infected through oral dosing with teladorsagia circumcincta infective larvae (3 d per wk) and were supple ...201020023143
sensitization to inhaled ryegrass pollen by collateral priming in a murine model of allergic respiratory disease.mouse models of asthma suffer from the necessity to prime the animals by injections before respiratory exposure. our aim was to develop a mouse model that mimics the progression of human allergic disease upon low-dose inhaled allergen exposure.201020150741
ecotoxicological study on sediments of mai po marshes, hong kong using organisms and biomarkers.sediments from mai po ramsar site, hong kong were in general shown to be highly toxic based on the results of four toxicity tests (microtox solid-phase test, daphnia mortality test, algal [microcystis aeruginosa] growth inhibition test and ryegrass [lolium perenne] seed germination/root elongation test). sediment of the mudflat (which is open to deep bay, i.e., the pollution source) was the most toxic while sediment of gei wai 24g (an enclosed freshwater pond) was the least toxic. results of bio ...201020153057
clinical significance of cross-reactivity between tobacco and latex.allergen cross-reactivity between tobacco and other species of solanaceae family (tomato, potato, aubergine and egg plant) have been reported. we have recently studied ige response to tobacco in asthmatic patients sensitised to lolium perenne (perennial rye grass pollen) and have found that 30% of the tobacco responsive patients also have latex sensitisation.201020409631
bee pollen: a dangerous food for allergic children. identification of responsible allergens.bee pollen has been proposed as a food supplement, but it can be a dangerous food for people with allergy. we study an allergic reaction after ingestion of bee pollen in a 4-year-old boy who had developed rhinitis in the last spring and autumn.201020447747
heavy metal concentrations in plants and different harvestable parts: a soil-plant equilibrium model.a mathematical interaction model, validated by experimental results, was developed to modeling the metal uptake by plants and induced growth decrease, by knowing metal in soils. the model relates the dynamics of the uptake of metals from soil to plants. also, two types of relationships are tested: total and available metal content. the model successfully fitted the experimental data and made it possible to predict the threshold values of total mortality with a satisfactory approach. data are tak ...201020605666
selecting tolerant grass seedlings and analyzing the possibility for using aged refuse as sward soil.in order to test the possibility for recycling use of aged refuse as sward soil, the study determined the responses of lolium perenne l. (perennial ryegrass), festuca arundinacea (tall fescue), and poa annua (annual bluegrass) to its leaching. the growth of three seedlings was significantly inhibited after treatment, especially for longer treatment duration and higher concentration leaching; however, with the better growth and chlorophyll content for shorter time and lower concentration, tall fe ...201020036424
towards a better understanding of the generation of fructan structure diversity in plants: molecular and functional characterization of a sucrose:fructan 6-fructosyltransferase (6-sft) cdna from perennial ryegrass (lolium perenne).the main storage compounds in lolium perenne are fructans with prevailing β(2-6) linkages. a cdna library of l. perenne was screened using poa secunda sucrose:fructan 6-fructosyltransferase (6-sft) as a probe. a full-length lp6-sft clone was isolated as shown by heterologous expression in pichia pastoris. high levels of lp6-sft transcription were found in the growth zone of elongating leaves and in mature leaf sheaths where fructans are synthesized. upon fructan synthesis induction, lp6-sft tran ...201021196473
consistency of crown rust evaluation in ryegrass cultivars.crown rust (puccinia coronata) is the most important leaf disease in forage ryegrasses (lolium spp.). in order to evaluate the stability in space and time of crown rust resistance a multisite rust evaluation trial was established by the european breeders association eucarpia ( fodder crops section). the same seed lots of 33 perennial ryegrass (lolium perenne) and 18 italian ryegrass (lolium multiflorum) varieties were sown in april 2001, 2004 and 2007 at 27 european sites. this paper reports the ...201021534471
ice recrystallization inhibition proteins of perennial ryegrass enhance freezing tolerance.ice recrystallization inhibition (iri) proteins are thought to play an important role in conferring freezing tolerance in plants. two genes encoding iri proteins, lpiri-a and lpiri-b, were isolated from a relatively cold-tolerant perennial ryegrass cv. caddyshack. amino acid alignments among the iri proteins revealed the presence of conserved repetitive iri-domain motifs (nxvxxg/nxvxg) in both proteins. quantitative reverse transcriptase pcr (qrt-pcr) analysis indicated that lpiri-a was up-regul ...201020379831
fungal endophytes of native grasses decrease insect herbivore preference and performance.endophytic fungal symbionts of grasses are well known for their protective benefit of herbivory reduction. however, the majority of studies on endophyte-grass symbioses have been conducted on economically important, agricultural species-particularly tall fescue (lolium arundinaceum) and perennial ryegrass (lolium perenne)-raising the hypothesis that strong benefits are the product of artificial selection. we examined whether fungal endophytes found in natural populations of native grass species ...201020585809
transforming a fructan:fructan 6g-fructosyltransferase from perennial ryegrass into a sucrose:sucrose 1-fructosyltransferase.fructosyltransferases (fts) synthesize fructans, fructose polymers accumulating in economically important cool-season grasses and cereals. fts might be crucial for plant survival under stress conditions in species in which fructans represent the major form of reserve carbohydrate, such as perennial ryegrass (lolium perenne). two ft types can be distinguished: those using sucrose (s-type enzymes: sucrose:sucrose 1-fructosyltransferase [1-sst], sucrose:fructan 6-fructosyltransferase) and those usi ...200918952861
demonstration of pathotype specificity in stem rust of perennial ryegrass.rust diseases cause significant damage in forage and seed crops of perennial ryegrass (lolium perenne), which is highly heterozygous and heterogeneous and thus presents difficulty in genetic analysis. there has been no definitive demonstration of the existence of pathotypes for stem rust or other rusts of perennial ryegrass, although experiments with crown rust (puccinia coronata) of this host are strongly suggestive of pathotype specificity. we made single-pustule isolates of p. graminis subsp. ...200919740032
phosphate addition and plant species alters microbial community structure in acidic upland grassland soil.agricultural improvement (addition of fertilizers, liming) of seminatural acidic grasslands across ireland and the uk has resulted in significant shifts in floristic composition, soil chemistry, and microbial community structure. although several factors have been proposed as responsible for driving shifts in microbial communities, the exact causes of such changes are not well defined. phosphate was added to grassland microcosms to investigate the effect on fungal and bacterial communities. plan ...200918581037
a novel nanoparticulate adjuvant for immunotherapy with lolium perenne.specific immunotherapy implies certain drawbacks which could be minimized by the use of appropriate adjuvants, capable of amplifying the right immune response with minimal side effects. in this context, previous studies of our group have demonstrated the adjuvant capacity of gantrez an nanoparticles, which can effectively enhance the immune response. in this work, two types of nanoparticles (with and without lps of brucella ovis as immunomodulator) with encapsulated lolium perenne extract are te ...200919545572
evaluation of the efficiency of a phytostabilization process with biological indicators of soil health.a phytostabilization process that combined the addition of a synthetic (calcinit + urea + pk14% + calcium carbonate) or organic (cow slurry) amendment with lolium perenne l. growth was used to remediate a mine soil moderately contaminated with zn, pb, and cd. the reduced toxicity caused by both amendments allowed the establishment of a healthy l. perenne vegetation cover that had a positive influence on soil properties, increasing the biomass, activity, and functional diversity of the soil micro ...200919704147
evaluation of molecular basis of cross reactivity between rye and bermuda grass pollen allergens.allergenic cross reactivity between the members of the pooids (lolium perenne, phleum pratense, and poa pratensis) and chloridoids (cynodon dactylon and paspalum notatum) is well established. studies using crude extracts in the past have demonstrated limited cross reactivity between the pooids and the chloridoids suggesting separate diagnosis and therapy. however, little is known regarding the molecular basis for the limited cross reactivity observed between the 2 groups of grasses. the present ...200919776679
rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts.within this study, we investigated whether the polyunsaturated fatty acids (pufa)-rich nature of rumen protozoa is a consequence of ingestion of pufa-rich chloroplasts. four hereford x friesian steers were offered hay [low 18:3 (n-3) and low chlorophyll concentration] followed by freshly cut perennial ryegrass [high 18:3 (n-3) and high chlorophyll concentration] for 16 days. on the 14th and 16th days, rumen protozoa as well as attached and planktonic bacteria were fractionated 1 h before (-1 h), ...200919583786
restricting time at pasture: effects on dairy cow herbage intake, foraging behavior, hunger-related hormones, and metabolite concentration during the first grazing session.this study investigated the effect of restricting grazing time on circulating concentrations of ghrelin, nonesterified fatty acids (nefa), and glucose before, and foraging behavior of dairy cows during, the first grazing session of the day (gs, 0800-1200 h). forty-eight holstein-friesian cows (470 +/- 47 kg of bw; 35 +/- 9 d in milk) were strip-grazed on a perennial ryegrass pasture for either 4 h after each milking (2 x 4), 8 h between milkings (1 x 8), or the 24-h period excluding milking time ...200919700720
restricting daily time at pasture at low and high pasture allowance: effects on pasture intake and behavioral adaptation of lactating dairy cows.in pasture-based dairy systems, daily time at pasture is restricted during several periods of the year. the aim of this experiment was to evaluate the effect of restricting time at pasture on milk yield, pasture dry matter (dm) intake, and grazing behavior of dairy cows according to pasture allowance (pa), which partly defines pasture availability. the experiment was carried out in spring on strip-grazed perennial ryegrass pastures. the 6 treatments consisted of 3 durations of daily time at past ...200919528610
earthworm and belowground competition effects on plant productivity in a plant diversity gradient.diversity is one major factor driving plant productivity in temperate grasslands. although decomposers like earthworms are known to affect plant productivity, interacting effects of plant diversity and earthworms on plant productivity have been neglected in field studies. we investigated in the field the effects of earthworms on plant productivity, their interaction with plant species and functional group richness, and their effects on belowground plant competition. in the framework of the jena ...200919526252
effect of flow parameters of rumen digesta on effective degradability and microbial yield in sheep.the aim of this experiment was to examine the effect of rumen digesta flow parameters on effective degradability (edg) and microbial nitrogen (mbn) yield in sheep fed diets of identical provision of both metabolizable energy and rumen degradable nitrogen (rdn). ruminal degradation parameters of early-harvested perennial ryegrass hay (eh), late-harvested perennial ryegrasses hay (lh) and winter sown barley straw (bs) were determined by a nylon bag technique. subsequently, three experimental diets ...200920163598
intercropped oats (avena sativa) - common vetch (vicia sativa) silage in the dry season for small-scale dairy systems in the highlands of central mexico.small-scale dairy systems in the highlands of central mexico require feeding strategies based on quality home-grown forage that may reduce high concentrate costs. eight holstein cows paired by parity and date of calving were used in a split-plot experiment to evaluate supplementing 6 kg dm/cow/d of oat-vetch silage (ovs) in comparison to maize silage (ms) as dry season feeding, for a more intensive use of the land through an oat-vetch catch crop. cows had 9 h/d access to continuous grazing of pe ...200919002599
organic residues as immobilizing agents in aided phytostabilization: (ii) effects on soil biochemical and ecotoxicological characteristics.sewage sludge (ss), municipal solid waste compost, and garden waste compost (gwc) were used as immobilizing agents in aided phytostabilization of an acid metal-contaminated soil affected by mining activities. the organic residues were applied at 25, 50 and 100 mg ha(-1) (dry weight basis) and perennial ryegrass (lolium perenne l.) was used as a "green cover". we studied the effects of the treatments on soil phytotoxicity and enzymatic activities, and on the composition and toxicity of the soil l ...200919091381
carryover of perfluorooctanoic acid (pfoa) and perfluorooctane sulfonate (pfos) from soil to plants.within the scope of a joint project to study soil-to-plant carryover of polyfluorinated compounds (pfcs), five cultivated plants (spring wheat, oats, potatoes, maize, and perennial ryegrass) were sown or planted in mitscherlich pots. six variants per species were used, each with a different concentration level of pfoa and pfos (from 0.25 to 50 mg/kg as aqueous solution) to detect possible concentration dependence in the transfer of these two pfcs from soil to plant. pfoa and pfos were detected b ...200919112561
assessment of very long-chain fatty acids as complementary or alternative natural fecal markers to n-alkanes for estimating diet composition of goats feeding on mixed diets.this study aimed to assess the use of very long-chain fatty acids (vlcfa) as an alternative or a complement to n-alkane markers for estimation of diet composition of goats fed 7 different diets, composed of different proportions of herbaceous (lolium perenne and trifolium repens) and heathland woody species (erica umbellata, erica cinerea, calluna vulgaris, erica arborea, and ulex gallii), in a metabolism study. diet composition was estimated from vlcfa (i.e., c(22) to c(34)) and alkane (i.e., c ...200919395523
assessment of dietary ratios of red clover and grass silages on milk production and milk quality in dairy cows.twenty-four multiparous holstein-friesian dairy cows were used in a replicated 4 x 4 latin square changeover design experiment to test the effects of changing from ryegrass (lolium perenne) silage to red clover (trifolium pratense) silage in graded proportions on feed intakes, milk production, milk organoleptic qualities, and whole-body nitrogen partitioning. four dietary treatments, comprising ad libitum access to 1 of 4 forage mixtures plus a standard allowance of 4 kg/d dairy concentrates, we ...200919233807
degradation of fuel oil in salt marsh soils affected by the prestige oil spill.we assessed natural degradation of fuel oil in three marshes from galicia (spain) affected by the prestige oil spill (baldaio, barizo, and muxía). soil samples collected from polluted and unpolluted areas on four different dates were used to determine total petroleum hydrocarbon content and fuel-oil components. natural degradation was monitored by analysing changes in the proportion of saturated hydrocarbons, aromatics, asphaltenes and resins in the soils, and also by evaluating the degree of de ...200919157704
influence of nitrogen fertilizer and endophyte infection on ecophysiological parameters and mineral element content of perennial ryegrass.an experiment was designed to determine the effect of the fungal endophyte neotyphodium lolii on the growth, physiological parameters and mineral element content of perennial ryegrass (lolium perennel l.), when growing at two n supply levels. endophyte infection had a significant positive effect on both shoot and root growth of ryegrass, but this difference was only significant in the high n supply treatment. at high n supply, endophyte-infected (ei) plants accumulated more soluble sugar in the ...200919166497
indole-diterpene biosynthetic capability of epichloë endophytes as predicted by ltm gene analysis.bioprotective alkaloids produced by epichloë and closely related asexual neotyphodium fungal endophytes protect their grass hosts from insect and mammalian herbivory. one class of these compounds, known for antimammalian toxicity, is the indole-diterpenes. the ltm locus of neotyphodium lolii (lp19) and epichloë festuce (fl1), required for the biosynthesis of the indole-diterpene lolitrem, consists of 10 ltm genes. we have used pcr and southern analysis to screen a broad taxonomic range of 44 end ...200919181837
identification of the ice-binding face of a plant antifreeze protein.the antifreeze protein of lolium perenne, a perennial ryegrass, was previously modeled as a beta-roll with two extensive flat beta-sheets on opposite sides of the molecule. here we have validated the model with a series of nine site-directed steric mutations in which outward-pointing short side-chain residues were replaced by tyrosine. none of these disrupted the fold. mutations on one of the beta-sheets and on the sides of the protein retained 70% or greater activity. three mutations that clust ...200919185572
phytoremediation of metal-contaminated soil in temperate humid regions of british columbia, canada.the suitability of five plant species was studied for phytoextraction and phytostabilisation in a region with temperate maritime climate of coastal british columbia, canada. pot experiments were conducted using lolium perenne l (perennial rye grass), festuca rubra l (creeping red fescue), helianthus annuus l (sunflower), poa pratensis l (kentucky bluegrass) and brassica napus l (rape) in soils treated with three different metal (cu, pb, mn, and zn) concentrations. the bio-metric characters of pl ...200919810356
mercury contamination in the vicinity of a chlor-alkali plant and potential risks to local population.a mercury-cell chlor-alkali plant operated in estarreja (north-western portugal) for 50 years causing widespread environmental contamination. although production by this process ceased in 2002, mercury contamination from the plant remains significant. the main objective of this study was to investigate mercury impact on the nearby environment and potential risks to local population. to assess the level of contamination soil samples were collected from agricultural fields in the vicinity of the p ...200919211131
increasing amounts of crushed wheat fed with pasture hay reduced dietary fiber digestibility in lactating dairy cows.sixteen cows in mid-lactation (milk yield of 23.8 +/- 2.3 kg/d) were individually fed diets consisting of chopped perennial ryegrass hay, offered at 3 kg of dry matter (dm)/100 kg of body weight (bw), fed either alone or supplemented with amounts of crushed wheat ranging from 0.4 to 1.6 kg of dm/100 kg of bw (increasing at nominal intervals of 0.4 kg of dm/100 kg of bw; 5 nominal treatments in total). three cows were allocated to each treatment except the mid-range wheat treatment, which had 4 c ...200919448009
comparison of sensitivity of grasses (lolium perenne l. and festuca rubra l.) and lettuce (lactuca sativa l.) exposed to water contaminated with microcystins.the effects of aqueous extracts from microcysts aeruginosa strains (both microcystin-producers and non-microcystin producers) on germination and root growth were investigated for three economically important plant species: festuca rubra l., lolium perenne l., and lactuca sativa l. there was a clear inhibition of root growth for l. sativa exposed to strains containing microcystins (5.9-56.4 microg l(-1)). the strain that produced the most pronounced effects contained the lowest concentration of m ...200919436926
activation of sucrose transport in defoliated lolium perenne l.: an example of apoplastic phloem loading plasticity.the pathway of carbon phloem loading was examined in leaf tissues of the forage grass lolium perenne. the effect of defoliation (leaf blade removal) on sucrose transport capacity was assessed in leaf sheaths as the major carbon source for regrowth. the pathway of carbon transport was assessed via a combination of electron microscopy, plasmolysis experiments and plasma membrane vesicles (pmvs) purified by aqueous two-phase partitioning from the microsomal fraction. results support an apoplastic p ...200919520670
two sides of a leaf blade: blumeria graminis needs chemical cues in cuticular waxes of lolium perenne for germination and differentiation.plant surface characteristics were repeatedly shown to play a pivotal role in plant-pathogen interactions. the abaxial leaf surface of perennial ryegrass (lolium perenne) is extremely glossy and wettable compared to the glaucous and more hydrophobic adaxial surface. earlier investigations have demonstrated that the abaxial leaf surface was rarely infected by powdery mildew (blumeria graminis), even when the adaxial surface was densely colonized. this led to the assumption that components of the ...200919352695
allergenicity and cross-reactivity of pine pollen.pine pollen has long been considered a non-allergenic pollen. the large size of the grain and its low levels of proteins are the main reasons invoked to explain this low allergenicity. the aim of this study was to describe the main allergenic bands of pinus radiata (pr) and its cross-reactivity with other pine species, other conifers and grass pollen.200919573163
effect of supplementation of grazing dairy ewes with a cereal concentrate on animal performance and milk fatty acid profile.this work was conducted to investigate the effect of supplementing grazing ewes on pasture with a cereal concentrate on the milk fatty acid (fa) profile. ninety assaf ewes in mid lactation were distributed in 9 lots of 10 animals each and allocated to 3 feeding regimens: 1) pasture--ewes were only allowed to graze pasture (an irrigated sward of lolium perenne, trifolium pratense, and dactylis glomerata); 2) ps--grazing ewes were supplemented with oat grain (700 g/animal and day); and 3) tmr--ewe ...200919620680
field assessment of a model for fungicide effects on intraplant spread of stem rust in perennial ryegrass seed crops.intraplant spread of stem rust (puccinia graminis subsp. graminicola) in perennial ryegrass during tiller extension is a major determinant of epidemic severity and is dominated by stem extension dynamics. simple equations for extension of inflorescence and internodes are presented and parameterized. these equations are combined with previously published equations for pathogen latent period and for postinfection efficacy of fungicides to produce a model for effects of fungicide type and timing on ...200919453228
enhanced phytoextraction of uranium and selected heavy metals by indian mustard and ryegrass using biodegradable soil amendments.the applicability of biodegradable amendments in phytoremediation to increase the uptake of uranium (u), cadmium (cd), chromium (cr), copper (cu), lead (pb) and zinc (zn) by indian mustard (brassica juncea) and ryegrass (lolium perenne) was tested in a greenhouse experiment. plants were cultivated during one month on two soils with naturally or industrially increased contaminant levels of u. treatments with citric acid, nh4-citrate/citric acid, oxalic acid, s,s-ethylenediamine disuccinic acid (e ...200919054545
effect of rumen fill on intake of fresh perennial ryegrass in young and mature dairy cows grazing or zero-grazing fresh perennial ryegrass.rumen fill may be a strong intake constraint for dairy cows fed on pasture, even though pasture is highly digestible in the grasslands of temperate climates. this constraint may also depend on the cows' maturity. moreover, indoor feeding of fresh herbage may not always be a good model for the study of intake regulation at grazing. to test these hypotheses, four mature (6.3 ± 0.72 year old) and four young (3.8 ± 0.20 year old) dairy cows were offered fresh perennial ryegrass indoors or at grazing ...200922443555
digestion during continuous culture fermentation when replacing perennial ryegrass with barley and steam-flaked corn.the objective of this study was to quantify the optimal inclusion rate of grain required to maximize nutrient digestion of a diet based on highly digestible pasture. it was hypothesized that maximum digestion would occur at a rate of grain inclusion that resulted in a culture ph of 6.0, reflecting the ph below which fiber digestion would be expected to be compromised. four dual-flow continuous culture fermenters were used to establish the effects on digestion of replacing freeze-dried, highly di ...200919109278
biodiversity effects on yield and unsown species invasion in a temperate forage ecosystem.current agricultural practices are based on growing monocultures or binary mixtures over large areas, with a resultant impoverishing effect on biodiversity at several trophic levels. the effects of increasing the biodiversity of a sward mixture on dry matter yield and unsown species invasion were studied.200919168861
cross-species amplification tests and diversity analysis using 56 pcr markers in dactylis glomerata and lolium perenne.we report results of cross-species amplification in dactylis glomerata and lolium perenne of 12 simple sequence repeats (ssrs) isolated from lolium multiflorum×festuca glaucescens, 42 ssrs from festuca arundinacea and two sequence tagged sites from oryza sativa. we compared the transferability and diversity between d. glomerata and l. perenne, which are important forage crops. while nei's gene diversity values were equivalent in both species (from 0.14 to 0.92), the mean number of allele per loc ...200921564590
[specific skin reactions induced by individual pollen preparations in hypersensitivity persons].skin tests are the most useful single modality for demonstrating an ige-mediated mechanism underlying clinical symptoms. skin reactivity to an allergen depends on person's exposure and genetic factors influencing the ige response. however, the reproducibility of allergy skin tests has been shown to be variable depending, among other things, on the allergen extract employed and the kind of technique used. these variations have led to controversy regarding clinical relevancy of allergy skin testin ...20099863408
characterization of allergenic epitopes of ory s1 protein from oryza sativa and its homologs.vaccination is the most effective technique suggested now days for allergy treatment. recombinant-based approaches are mostly focused on genetic modification of allergens to produce molecules with reduced allergenic activity and conserved antigenicity. the molecules developed for vaccination in allergy possess significantly reduced allergenicity in terms of ige binding, and therefore will not lead to anaphylactic reactions upon injection. this approach is probably feasible with every peptide all ...200920011147
feasibility of incorporating waste grass clippings (lolium perenne l.) in particleboard composites.this study investigated some of the important physical (thickness swelling) and mechanical (modulus of rupture, modulus of elasticity and internal bond) properties of single-layer particleboard panels made from eucalyptus (eucalyptus camaldulensis dehn.), waste of grass clippings (lolium perenne l.) and combinations of the two. the chemical properties (ph, holocelluse and alpha cellulose contents, and water, alcohol-benzene and 1% sodium hydroxide solubilities) of the raw materials were also det ...200918804991
interception of spray drift by border structures. part 2: field experiments.this research studied the effect of drift-intercepting structures surrounding the field borders, like artificial screens and natural hedges, which are not yet a part of the drift mitigation measures for field crop sprayers in belgium. drift-interception experiments were performed in a grassland (lolium perenne) with various interception structures: artificial screens with heights of 1, 1.5 and 2 m and screen open areas of 16, 36 and 63% and a row of fagus sylvatica trees with a height of 1.5 m a ...200819226820
effects, uptake, and fate of 2,4,6-trinitrotoluene aged in soil in plants and worms.the present study was aimed at providing data to be used at predicting exposure-based effects of 2,4,6-trinitrotoluene (tnt) aged in soil on endpoint organisms representing two trophic levels. these data can be used to define criteria or reference values for environmental management and conducting specific risk assessment. long-term exposure tests were conducted to evaluate sublethal toxicity and uptake of aged soil-based explosives, with tnt as the main contaminant. in these tests, plants were ...200818620472
e/z-thesinine-o-4'-alpha-rhamnoside, pyrrolizidine conjugates produced by grasses (poaceae).based on direct infusion mass spectrometry we identified a novel alkaloid as a major component of perennial ryegrass (lolium perenne). initial mass spectral data suggested it to be a pyrrolizidine conjugate. as this class of alkaloids has not been described before from grasses, we isolated it to elucidate its structure. the isolated alkaloid proved to be a mixture of two stereoisomers. the structures of the two compounds as determined by 1d and 2d nmr spectroscopy, were e-thesinine-o-4'-alpha-rh ...200818466931
nitrate losses, nutrients and heavy metal accumulation from substrates assembled for urban soils reconstruction.urban soils may suffer mild to severe degradation as a result of physical and chemical alterations. to reconstruct these soils, a new upper horizon must be created, usually through the application of organic matter, one source of which is biosolids. different soil mixtures were evaluated with regard to loss of nitrates in percolates and the uptake and incorporation of nutrients and heavy metals into plant tissues. the experiment was conducted in trays; treatments were mixtures of biosolids and a ...200817904270
grazing behaviour and performance of lactating suckler cows, ewes and goats on partially improved heathlands.the foraging behaviour (grazing time and diet composition), live-weight (lw) changes and parasitic infection (faecal egg counts of gastrointestinal nematodes) of 12 beef cows, 84 ewes and 84 goats suckling their offspring, managed in mixed grazing on heathlands with 24% of improved pasture of perennial ryegrass-white clover, were studied during the years 2003 and 2006. the results showed that goats tended to graze for a longer time, and utilised significantly more heathland vegetation than cattl ...200822444089
direct fermentation of fodder maize, chicory fructans and perennial ryegrass to hydrogen using mixed microflora.this study examined the feasibility of producing hydrogen by direct fermentation of fodder maize, chicory fructooligosaccharides and perennial ryegrass (lolium perenne) in batch culture (ph 5.2-5.3, 35 degrees c, heat-treated anaerobically digested sludge inoculum). gas was produced from each substrate and contained up to 50-80% hydrogen during the peak periods of gas production with the remainder carbon dioxide. hydrogen yields obtained were 62.4+/-6.1ml/g dry matter added for fodder maize, 218 ...200818513959
conventional crops and organic amendments for pb, cd and zn treatment at a severely contaminated site.the ability of selected plants and amendments to treat pb, cd and zn accumulations from a metalliferous waste disposal site was studied both in the greenhouse and field. spinach (spinacea oleracea), cabbage (brassica oleracea), and a grass-legume mix (red fescue, festuca rubra; ryegrass, lolium perenne); and bean (vicia faba) were grown in the greenhouse on blast furnace slag or baghouse dust amended with composted peat (cp). all plant species accumulated pb, cd and zn to varying degrees. total ...200817475483
phytotoxic effects of sewage sludge extracts on the germination of three plant species.in order to evaluate the ability of three types of extracts to explain the ecotoxicological risk of treated municipal sewage sludges, the oecd 208a germination test was applied using three plants (lolium perenne l., brassica rapa l., and trifolium pratense l.). three equivalent batches of sludge, remained as dewatered sludge, composted with plant remains and thermally dried, from an anaerobic waste water treatment plant were separated. samples from these three batches were extracted in water, me ...200818651217
distribution, biology and pathology of anguina pacificae.anguina pacificae is distributed along a narrow strip on the pacific coast of northern california where it forms galls on the shoots of poa annua and causes significant damage to golf course greens. methods were developed for the continuous propagation of a. pacificae on p. annua in growth chambers, and they were used to examine the life cycle and host-parasite relationships of the nematode. at a mean temperature of 20 degrees c (22 degrees c day/18 degrees c night) the life cycle was completed ...200819440264
phytoremediation of oil-sludge-contaminated soil.the aim of this research was to select plant species that could be effective in the phytoremediation ofa former oil-sludge pit. seven crop plants (triticum aestivum l., secale cereale l., avena sativa l., hordeum vulgare, sorghum bicolor l moench, panicum miliaceum l, and zea mays l.),five wild grasses (lolium perenne l., bromopsis inermis, agropyron cristatum l., agropyrum tenerum l., and festuca pratensis huds.), and three legumes (medicago sativa l., trifolium pratense l., and onobrychis anta ...200819260228
short communication: effect of postgrazing residual pasture height on milk production.in grazing systems, dry matter intake (dmi) and milk production have been reported to increase with increasing pasture allowance (pa). this has often led to greater postgrazing residual heights being associated with a well-fed cow. however, in previous studies pastures were often managed to be homogeneous pretreatment, confounding the effect of postgrazing height and pa because high pa led to high postgrazing height. the objective of this study was to determine whether postgrazing height affects ...200818946136
trophic cascades initiated by fungal plant endosymbionts impair reproductive performance of parasitoids in the second generation.variation in plant quality can transmit up the food chain and may affect herbivores and their antagonists in the same direction. fungal endosymbionts of grasses change the resource quality by producing toxins. we used an aphid-parasitoid model system to explore how endophyte effects cascade up the food chain and influence individual parasitoid performance. we show that the presence of an endophyte in the grass lolium perenne has a much stronger negative impact on the performance of the parasitoi ...200818636277
animal performance and economic comparison of novel and toxic endophyte tall fescues to cool-season annuals.increased costs of annual establishment of small grain pasture associated with fuel, machinery, and labor are eroding the profitability of stocker cattle enterprises. interest has therefore increased in development of cool-season perennial grasses that are persistent and high quality. this study occurred on 24 ha (divided into thirty 0.81-ha paddocks) located at the university of arkansas division of agriculture livestock and forestry branch station, near batesville. two tall fescue (festuca aru ...200818310498
effects of increasing days of exposure to prepartum transition diets on milk production and milk composition in dairy cows.to evaluate the effects of length of exposure to prepartum transition diets on milk yield, fat and protein production.200818782415
the molecular mechanism of "ryegrass staggers," a neurological disorder of k+ channels."ryegrass staggers" is a neurological condition of unknown mechanism that impairs motor function in livestock. it is caused by infection of perennial ryegrass pastures by an endophytic fungus that produces neurotoxins, predominantly the indole-diterpenoid compound lolitrem b. animals grazing on such pastures develop uncontrollable tremors and become uncoordinated in their movement. lolitrem b and the structurally related tremor inducer paxilline both act as potent large conductance calcium-activ ...200818801945
characterization of a family of ice-active proteins from the ryegrass, lolium perenne.five genes coding for ice-active proteins were identified from an expressed sequence tag database of lolium perenne cdna libraries. each of the five genes were characterized by the presence of an n-terminal signal peptide, a region enriched in hydrophilic amino acids and a leucine-rich region in four of the five genes that is homologous with the receptor domain of receptor-like protein kinases of plants. the c-terminal region of all five genes contains sequence homologous with lolium and triticu ...200818835384
screening for soil streptomycetes from north jordan that can produce herbicidal compounds.a total of 231 different soil streptomyces isolates were recovered from 16 different locations in north jordan. they were assessed for their phytotoxic activity on seeds of cucumber (cucumis sativus l.) and ryegrass (lolium perenne l.) placed adjacent to a 2 cm wide streptomyces culture strips grown at 28c degrees for 3 weeks on starch casein nitrate (scn) agar. phytotoxicity was ascertained on the basis of suppressed seed germination, discoloration of the root tip, reduced root and the shoot gr ...200819275043
plant-endophyte-herbivore interactions: more than just alkaloids?a recent paper by rasmussen et al., (new phytol 2007; 173:787-97) describes the interactions between lolium perenne cultivars with contrasting carbohydrate content and the symbiotic fungal endophyte neotyphodium lolii at different levels of nitrogen supply. in a subsequent study undertaken by rasmussen et al., (plant physiol 2008; 146:1440-53) 66 metabolic variables were analysed in the same material, revealing widespread effects of endophyte infection, n supply and cultivar carbohydrate content ...200819704424
metabolic profiles of lolium perenne are differentially affected by nitrogen supply, carbohydrate content, and fungal endophyte infection.lolium perenne cultivars differing in their capacity to accumulate water soluble carbohydrates (wscs) were infected with three strains of fungal neotyphodium lolii endophytes or left uninfected. the endophyte strains differed in their alkaloid profiles. plants were grown at two different levels of nitrogen (n) supply in a controlled environment. metabolic profiles of blades were analyzed using a variety of analytical methods. a total of 66 response variables were subjected to a principle compone ...200818218971
maldi-tof ms analysis of labile lolium perenne major allergens in mixes.it is well known that allergen extracts used for specific therapy of allergic disorders are commonly stored as mixtures, causing an alteration of its stability.200818503569
toxic effects of digested, composted and thermally-dried sewage sludge on three plants.in order to evaluate potential toxic effects of stabilized sewage sludge that are currently applied to agricultural soils, three types of municipal sewage sludge and one pig slurry were subjected to phytotoxicity assays using three plants (brassica rapa, lolium perenne and trifolium pratense). equivalent batches of aerobically and anaerobically-digested sludge (f) from two municipal wastewater treatment plants, were composted (c) or thermally dried (t). in addition, one anaerobically-digested an ...200818280152
antigenic similarity among group 1 allergens from grasses and quantitation elisa using monoclonal antibodies to phl p 1.group 1 allergens elicit a specific ige response in about 90% of grass pollen-allergic patients. the aim of this work was to study the antigenic similarity among group 1 allergens from different grasses and to develop a monoclonal antibody (mab)-based quantitation elisa.200818004069
dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen.anaerobic fungi (neocallimastigales) are active degraders of fibrous plant material in the rumen. however, only limited information is available relating to how quickly they colonize ingested feed particles. the aim of this study was to determine the dynamics of initial colonization of forage by anaerobic fungi in the rumen and the impact of different postsampling wash procedures used to remove loosely associated microorganisms. neocallimastigales-specific molecular techniques were optimized to ...200818673390
nano-aluminum: transport through sand columns and environmental effects on plants and soil communities.nano-aluminum is being used in increasing quantities as energetic material. this research addresses the transport of two types of nanosized aluminum particles (with aluminum oxide, or carboxylate ligand coating, alex and l-alex, respectively) through sand columns along with associated environmental impacts on soil systems. surface phenomena and ph are variables controlling the transport of nano-aluminum particles through porous media. ph environment controls solubility and electrostatic interact ...200817537426
advanced data-mining strategies for the analysis of direct-infusion ion trap mass spectrometry data from the association of perennial ryegrass with its endophytic fungus, neotyphodium lolii.direct-infusion mass spectrometry (ms) was applied to study the metabolic effects of the symbiosis between the endophytic fungus neotyphodium lolii and its host perennial ryegrass (lolium perenne) in three different tissues (immature leaf, blade, and sheath). unbiased direct-infusion ms using a linear ion trap mass spectrometer allowed metabolic effects to be determined free of any preconceptions and in a high-throughput fashion. not only the full ms(1) mass spectra (range 150-1,000 mass-to-char ...200818287492
natural enemies act faster than endophytic fungi in population control of cereal aphids.1. fast-growing populations of phytophagous insects can be limited by the presence of natural enemies and by alkaloids that are produced by symbiotic associations of many temperate grass species with endophytic fungi. it is unclear if and how acquired plant defences derived from endophytic fungi interact with natural enemies to affect phytophagous insect populations. 2. to assess the relative importance of endophytic fungi compared to that of natural enemies on the population dynamics of phytoph ...200818312338
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