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further characterization of a new recombinant group of plum pox virus isolates, ppv-t, found in orchards in the ankara province of turkey.sixteen plum pox virus (ppv) isolates collected in the ankara region of turkey were analyzed using available serological and molecular typing assays. surprisingly, despite the fact that all isolates except one, which was a mix infection, were typed as belonging to the ppv-m strain in four independent molecular assays, nine of them (60%) reacted with both ppv-m specific and ppv-d specific monoclonal antibodies. partial 5' and 3' genomic sequence analysis on four isolates demonstrated that irrespe ...200919428744
direct sample preparation methods for the detection of plum pox virus by real-time rt-pcr.direct systems to process plant materials allowed high-throughput testing of plum pox virus (ppv) by real-time reverse transcription (rt)-pcr without nucleic acids purification. crude plant extracts were diluted in buffer or spotted on membranes to be used as templates. alternatively, immobilized ppv targets were amplified from fresh sections of plant tissues printed or squashed onto the same supports, without extract preparation. spot real-time rt-pcr was validated as a ppv diagnostic method in ...200919440977
bone marrow-derived endothelial progenitor cells contribute to the angiogenic switch in tumor growth and metastatic progression.emerging evidence indicates that bone marrow (bm)-derived endothelial progenitor cells (epcs) contribute to angiogenesis-mediated growth of certain tumors in mice and human. epcs regulate the angiogenic switch via paracrine secretion of proangiogenic growth factors and by direct luminal incorporation into sprouting nascent vessels. while the contributions of epcs to neovessel formation in spontaneous and transplanted tumors and to the metastatic transition have been reported to be relatively low ...200919460418
'kwanzan stunting' syndrome: detection and molecular characterization of an italian isolate of little cherry virus 1.evident stunting was observed for the first time on prunus serrulata 'kwanzan' indicator trees in southern italy during the indexing of two sour cherry accessions from cultivars 'marasca di verona' and 'spanska'. bud break and shooting were delayed and the developing leaves remained small. during the third year many kwanzan plants died, regardless of the indexed cultivar. electrophoretic analysis showed the presence of dsrna pattern in extracts of stunted kwanzan with a similar size to that of v ...200919463722
electroantennogram technique for conotrachelus nenuphar (coleoptera: curculionidae).reliable electroantennogram (eag) responses were obtained from the plum curculio, conotrachelus nenuphar (herbst), by using a whole body mount with glass electrodes filled with kcl-saturated agcl solution that penetrated the antennal club (recording electrode) and the membrane attached to the cervical sclerite between the thorax and the head (indifferent electrode). known attractive odor sources including extracts of headspace collections of 'stanley' plum and 'empire' apple tissues and a synthe ...200919508798
identifying peach and plum polyphenols with chemopreventive potential against estrogen-independent breast cancer cells.our objective was to evaluate the cancer suppression activity of extracts from a commercial variety of yellow-fleshed peach 'rich lady' (rl) and a red-fleshed plum 'black splendor' (bs) and identify the phenolic fractions that may possess potential as chemopreventive and/or chemotherapeutic natural compounds. the peach rl extract effectively inhibited the proliferation of the estrogen-independent mda-mb-435 breast cancer cell line. the concentration to inhibit 50% of cell proliferation (ic(50)) ...200919530711
[pricking blood therapy combined with ultraviolet irradiation for treatment of acute herpes zoster].to evaluate clinical therapeutic effect and the safety of pricking blood therapy combined with ultraviolet irradiation for treatment of acute herpes zoster.200919565736
observations of atmospheric nitrogen and phosphorus deposition during the period of algal bloom formation in northern lake taihu, china.cyanobacterial blooms in lake taihu occurred at the end of april 2007 and had crucial impacts on the livelihood of millions of people living there. excessive nutrients may promote bloom formation. atmospheric nitrogen (n) and phosphorus (p) deposition appears to play an important role in algal bloom formation. bulk deposition and rain water samples were collected respectively from may 1 to november 30, 2007, the period of optimal algal growth, to measure the bulk atmospheric deposition rate, wet ...200919597869
quantitative estimation of plum pox virus targets acquired and transmitted by a single myzus persicae.the viral charge acquired and inoculated by single aphids in a non-circulative transmission is estimated using plum pox virus (ppv). a combination of electrical penetration graph and taqman real-time rt-pcr techniques was used to establish the average number of ppv rna targets inoculated by an aphid in a single probe (26,750), approximately half of the acquired ones. this number of ppv targets is responsible for a systemic infection of 20% on the inoculated receptor plants. no significant differ ...200919597934
[parasitoids (hymenoptera: braconidae) of fruit flies (diptera: tephritidae) in the state of são paulo, brazil: associated plants and parasitism].a total of 3,008 braconid parasitoids in 26 species of fruit fly host plants were collected in 33 localities of são paulo state. the majority of the braconids (96.2%) belonged to four opiinae species, doryctobracon areolatus (szépligeti), doryctobracon brasiliensis (szépligeti), opius bellus (gahan) and utetes anastrephae (viereck), and the remaining 3.8%, a single species of alysiinae, asobara anastrephae (muesebeck). doryctobracon areolatus was collected on anastrepha fraterculus (wied), a. ob ...200919618046
lower limb deformity due to failed trauma treatment corrected with the ilizarov technique: factors affecting the complication rate in 52 patients.failed treatment of fractures may be corrected by the ilizarov technique but complications are common. in 52 patients with compromised healing of femoral and tibial fractures, the results of secondary reconstruction with ilizarov treatment were investigated retrospectively in order to identify the factors that contribute to the risk of complications.200919626469
induction of apoptosis by immature plum in human hepatocellular carcinoma.in the present study, the effect of an extract of immature prunus salicina lindl. cv. soldam fruit on the viability and induction of apoptosis in human hepatocellular carcinoma hepg2 cells was investigated. the results showed that in comparison with other cancer cells, the growth inhibition exerted by immature plum extracts was greatest in hepg2. apoptosis in hepg2 cells mediated by immature plums was associated with "death receptor signaling." immature plum extracts significantly increased the ...200919627199
analysis of pesticide residues by fast gas chromatography in combination with negative chemical ionization mass spectrometry.a combination of fast gc with narrow-bore column and bench top quadrupole mass spectrometer (ms) detector in negative chemical ionization (nci) mode (with methane as reagent gas) is set up and utilized for the ultratrace analysis of 25 selected pesticides. the observed pesticides, belonging to the endocrine disrupting chemicals (edcs), were from different chemical classes. a comparative study with electron impact (ei) ionization was also carried out (both techniques in selected ion monitoring (s ...200919643423
quality of life in simultaneous pancreas-kidney transplant recipients.simultaneous pancreas-kidney transplantation (spk tx) allows dialysis and insulin therapy to be discontinued and improves the complications of diabetes mellitus type 1 (dm1). this study measure quality of life (qol) in spk transplant recipients and determine if there are differences in qol between these patients and those with dm1 in renal replacement therapy (rrt).200919674015
field efficacy of entomopathogenic fungi and nematodes targeting caged last-instar plum curculio (coleoptera: curculionidae) in michigan cherry and apple orchards.the plum curculio (conotrachelus nenuphar herbst) is a key pest of pome and stone fruit in eastern north america. we tested the efficacy of five pathogens over the course of three seasons in 10 michigan apple and cherry orchards, with introductions of larvae to caged pots containing pathogen-treated soil. the nematode steinernema riobrave was the most effective pathogen in the 2 yr it was tested, but only in soils with the highest sand content (81-88%) and when it was applied 1 h or 5 d after la ...200919689891
towards the routine use of brain imaging to aid the clinical diagnosis of disorders of consciousness.clinical audits have highlighted the many challenges and dilemmas faced by clinicians assessing persons with disorders of consciousness (vegetative state and minimally conscious state). the diagnostic decision-making process is highly subjective, dependent upon the skills of the examiner and invariably dictated by the patients' ability to move or speak. whilst a considerable amount has been learnt since jennett and plum coined the term 'vegetative state', the assessment process remains largely u ...200919710182
the determinant of potyvirus ability to overcome the rtm resistance of arabidopsis thaliana maps to the n-terminal region of the coat protein.in arabidopsis thaliana columbia (col-0) plants, the restriction of tobacco etch virus (tev) long-distance movement involves at least three dominant rtm (restricted tev movement) genes named rtm1, rtm2, and rtm3. previous work has established that, while the rtm-mediated resistance is also effective against other potyviruses, such as plum pox virus (ppv) and lettuce mosaic virus (lmv), some isolates of these viruses are able to overcome the rtm mechanism. in order to identify the viral determina ...200919737103
a new selective medium for the recovery and enumeration of monilinia fructicola, m. fructigena, and m. laxa from stone fruits.isolation of monilinia spp. from stone and pome fruit surfaces is difficult due to the presence of several fast-growing fungal species such as rhizopus, alternaria, and penicillium spp. therefore, a new selective medium (acidified potato dextrose agar [ph 3.6] amended with fosetyl-aluminum [fosetyl-al] at 500 microg/ml) (apda-f500) was developed for the recovery of monilinia propagules. the antifungal agents fosetyl-al, dichloran, ammonium molybdate, and 2-deoxy-d-glucose (2-dd-glucose) were tes ...200919740034
geographical location has greater impact on carotenoid content and bioaccessibility from tomatoes than variety.the suggested health benefits of consuming tomatoes and tomato-based products have been attributed, in part, to the carotenoids present in these foods. therefore, the objectives of the present study were to (i) analyse carotenoid content and bioaccessibility from different tomato (lycopersicon esculentum l.) types namely cherry, plum, round, and certain tomatoes-on-the-vine; and (ii) determine if geographical location (ireland vs spain) influenced the content and bioaccessibility of carotenoids ...200919757067
pesticide extraction from table grapes and plums using ionic liquid based dispersive liquid-liquid microextraction.room temperature ionic liquids (rtils) have been used as extraction solvents in dispersive liquid-liquid microextraction (dllme) for the determination of eight multi-class pesticides (i.e. thiophanate-methyl, carbofuran, carbaryl, tebuconazole, iprodione, oxyfluorfen, hexythiazox, and fenazaquin) in table grapes and plums. the developed method involves the combination of dllme and high-performance liquid chromatography with diode array detection. samples were first homogenized and extracted with ...200919779926
effectiveness of glues used for harmonic radar tag attachment and impact on survival and behavior of three insect pests.the ability of three cyanoacrylate glues to ensure a durable bond between the colorado potato beetle, leptinotarsa decemlineata (say), the plum curculio, conotrachelus nenuphar (herbst), or the corn rootworms (western corn rootworm, diabrotica virgifera virgifera leconte and northern corn rootworm, diabrotica longicornis smith and lawrence) and the harmonic radar tag without impact on behavior and survival was assessed as part of a study on the use of harmonic radar technology to track these ins ...200919791611
measurement of greenhouse gas emissions from agricultural sites using open-path optical remote sensing method.improved characterization of distributed emission sources of greenhouse gases such as methane from concentrated animal feeding operations require more accurate methods. one promising method is recently used by the usepa. it employs a vertical radial plume mapping (vrpm) algorithm using optical remote sensing techniques. we evaluated this method to estimate emission rates from simulated distributed methane sources. a scanning open-path tunable diode laser was used to collect path-integrated conce ...200919827493
a tale of two carboxypeptidases.proopiomelanocortin (pomc) neurons play a central role in energy homeostasis. despite the complexity of pomc posttranslational processing, regulation of pomc gene expression often takes center stage. complementary papers that zero in on distinct carboxypeptidases (plum et al., 2009; wallingford et al., 2009) now refocus the spotlight on regulated peptide cleavage.200919883612
curative activity of insecticides against plum curculio (coleoptera: curculionidae) in tart cherries.tart cherry, prunus cerasus l. variety montmorency, fruit were infested with plum curculio, conotrachelus nenuphar (herbst) (coleoptera: curculionidae), and treated with insecticides to target late instars, neonates, and eggs. the organophosphates azinphos-methyl and phosmet and the neonicotinoid thiamethoxam reduced larval emergence rates by >90% for all life stage targets; after >30 d, few surviving larvae were found inside fruit. acetamiprid and thiacloprid also had curative activity and yiel ...200919886451
analysis of phenolic compounds in six norwegian plum cultivars (prunus domestica l.).six european plum cultivars ( prunus domestica l.) grown in norway have been studied with respect to phenolic composition. neochlorogenic acid was found to be the most important phenolic acid in all cultivars. together with other phenolic acids, this compound varied significantly in amount among the cultivars. cyanidin 3-rutinoside was found to account for >60% of the total anthocyanin content. minor amounts of flavonols (rutin and quercetin 3-glucoside) were detected in all cultivars. total ant ...200919888727
immunostimulatory effects of oriental plum (prunus salicina lindl.).the fruit of the plum tree (prunus salicina lindl.) has been used as a traditional medicinal food in humans to enhance immunity against infectious agents and to treat cancers. however, limited information exists on the mechanisms responsible for its immune enhancing properties. in this study, the immunostimulatory effects of a methanol extract of plum fruit following methanol evaporation and dissolving in pbs were assessed by in vitro lymphocyte proliferation, tumor cell cytotoxicity, and nitric ...200918262269
to address accuracy and precision using methods from analytical chemistry and computational physics.in this work the pesticides were determined by liquid chromatography-mass spectrometry (lc-ms). in present study the occurrence of imidacloprid in 343 samples of oranges, tangerines, date plum, and watermelons from valencian community (spain) has been investigated. the nine additional pesticides were chosen as they have been recommended for orchard treatment together with imidacloprid. the mulliken population analysis has been applied to present the charge distribution in imidacloprid. partition ...200918369729
changes in blood pesticide levels in booted eagle (hieraaetus pennatus) associated with agricultural land practices.we estimated the degree of exposure to lindane and endosulfan in the blood of booted eagle nestlings (hieraaetus pennatus) (1999-2003), in order to assess the usefulness of these samples as a unit for monitoring changes in exposure as a result of shifts in agricultural practices and the implementation of legal measures. the highest blood lindane concentrations were obtained 1 year prior to its prohibition by the european union. subsequent to that year, the drop in blood concentrations was dramat ...200918417218
dried plum polyphenols attenuate the detrimental effects of tnf-alpha on osteoblast function coincident with up-regulation of runx2, osterix and igf-i.previous studies have demonstrated that dried plums which contain high amounts of polyphenols can restore bone mass and structure, and significantly increase indices of bone formation. the purpose of this study was to determine how dried plum polyphenols influence osteoblast activity and mineralized nodule formation under normal and inflammatory conditions. mc3t3-e1 cells were plated and pretreated with dried plum polyphenols (0, 2.5, 5, 10 and 20 microg/ml) and 24 h later stimulated with tnf-al ...200918495459
dried plum polyphenols inhibit osteoclastogenesis by downregulating nfatc1 and inflammatory mediators.dried plums and their polyphenols have been shown to suppress bone resorption by downregulating receptor activator nf-kappab ligand (rankl). due to the anti-inflammatory and antioxidant properties of these compounds, this study was designed to investigate whether dried plum polyphenols exert additional, more direct effects on osteoclasts and their precursors. raw 264.7 macrophages were used as a model to study osteoclast precursors and osteoclast differentiation and activity. under inflammatory ...200818509698
biosecurity. fate of plum island animal lab still unclear. 200818511663
differential regulation of four members of the acc synthase gene family in plum.the regulation of acc synthase (acs) genes was studied in early ('early golden') and late ('shiro') japanese plum cultivars (prunus salicina l.) in order to determine the role of this gene family in fruit ripening. of the four ps-acs cdnas isolated, two (ps-acs1 and -3) showed differential expression between the two cultivars. ps-acs1 accumulated during fruit ripening of 'early golden' ('eg') and 'shiro' ('sh') in ethylene-dependent and -independent manners, respectively. ps-acs3a transcripts ac ...200818535295
alteration in the chloroplastic metabolism leads to ros accumulation in pea plants in response to plum pox virus.in this work, a recombinant plum pox virus (ppv, sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. alaska). ppv had produced chlorotic spots as well as necrotic spots in the oldest leaves at 13-15 d post-inoculation. at 15 d post-inoculation, ppv was present in the chlorotic and necrotic areas, as shown by the fluorescence signal produced by the presence of the green fluorescent protein ...200818535298
respiratory allergy to lipid transfer protein.due to unclear reasons, allergy to lipid transfer protein (ltp) is frequent in mediterranean countries but rare in northern europe.200818535391
efficient amelioration of carbon tetrachloride induced toxicity in isolated rat hepatocytes by syzygium cumini skeels extract.syzygium cumini, indian black plum or java plum, is a rich source for anthocyanins (230 mg/100g dw) showing high antioxidant activity in vitro. in the following study it is further demonstrated that s. cumini peel extract rich in anthocyanins (sca) offers considerable protection against carbon tetrachloride (ccl(4))-induced damage in rat hepatocytes. sca itself being non-toxic to primary rat hepatocytes at concentrations ranging from 50 to 500 ppm, was found to suppress ccl(4)-induced ldh leakag ...200818538978
characterization and antioxidative properties of oligomeric proanthocyanidin from prunes, dried fruit of prunus domestica l.a prune extract was separated by an antioxidant-guided assay to obtain an oligomeric proanthocyanidin fraction. the antioxidative oligomer was characterized as a procyanidin oligomer with an average polymerization degree of five and composed of epicatechin and catechin units. the antioxidative activity of the oligomer showed greater potency than chlorogenic acid which is known as an antioxidative component in prunes.200818540093
characterization of plum procyanidins by thiolytic depolymerization.the phenolic compounds of 'green gage' (gg) plums ( prunus domestica l.), "rainha claudia verde", from a 'protected designation of origin' (pdo), in portugal, were quantified in both flesh and skin tissues of plums collected in two different orchards (gg-v and gg-c). analyzes of phenolic compounds were also performed on another gg european plum obtained in france (gg-f) and two other french plums, 'mirabelle' (m) and 'golden japan' (gj). thiolysis was used for the first time in the analysis of p ...200818540618
effect of seven different pollens on bio-ecological parameters of the predatory mite typhlodromus foenilis (acari: phytoseiidae).development, immature survival, reproduction, and life table parameters of the predatory mite typhlodromus foenilis oudemans were evaluated on seven different plant pollens (almond, apple, pear, cherry, apricot, plum, and walnut). experiments were conducted in environmental chambers at 20 +/- 1 degrees c with 65 +/- 5% rh and a photoperiod of 16:8 (l:d). t. foenilis successfully completed its development on all pollen diets. female mean developmental time from egg to adult emergence ranged from ...200818419905
phytotherapy for lower urinary tract symptoms secondary to benign prostatic hyperplasia.we examined the available data from clinical trials for certain botanicals used for lower urinary tract symptoms secondary to benign prostatic hyperplasia, including serenoa repens (saw palmetto), pygeum africanum (african plum), secale cereale (rye pollen) and hypoxis rooperi (south african star grass).200818423748
foot-and-mouth disease in north american bison (bison bison) and elk (cervus elaphus nelsoni): susceptibility, intra- and interspecies transmission, clinical signs, and lesions.there is limited information about the pathogenesis and epidemiology of foot-and-mouth disease (fmd) in north american bison (bison bison) or elk (cervus elaphus nelsoni). in these two experimental infection studies, we compared the susceptibilities of bison and elk to fmd virus (fmdv), respectively, with that of cattle; determined whether intra- and interspecies transmission could occur in bison and cattle, and elk and cattle; determined suitability of conventional available laboratory tests to ...200818436660
complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1.pineapple mealybug wilt-associated virus-1 (pmwav-1; family closteroviridae, genus ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. in this study, the complete genome of pmwav-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family. the genome encoded seven open reading frames (orfs) and was unusual for an ampelovirus due to the lack of an intergenic region between the rdrp and p6 orfs, an orf encoding a relatively ...200818283409
retention sites for xylella fastidiosa in four sharpshooter vectors (hemiptera: cicadellidae) analyzed by scanning electron microscopy.xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis (cvc), pierce's disease of grapevine, and leaf scald of coffee and plum and many other plant species. this pathogen is vectored by sharpshooter leafhoppers (hemiptera: cicadellidae: cicadellinae) and resides in the insect foregut. scanning electron microscopy was used to determine the retention sites of x. fastidiosa for the most common vector species in brazilian citrus groves, acrogonia citrina, bucephalogo ...200818288522
from dietary guidelines to daily food guide: the taiwanese experience.in early 1980's, the first edition of dietary guidelines for taiwanese was established by the department of health (doh), executive yuan. the main purposes of the guidelines were to guide nutritional professionals and dieticians to plan diet for preventing general population from diseases. besides the amount of daily cholesterol intake, the percentages of calories from carbohydrate, protein, and fat was recommended. dietary guidelines were revised concurrently as the results of national nutritio ...200818296302
in vitro inhibition of human influenza a virus infection by fruit-juice concentrate of japanese plum (prunus mume sieb. et zucc).using a plaque reduction assay, treatment of human influenza a viruses with the fruit-juice concentrate of japanese plum (prunus mume sieb. et zucc) showed strong in vitro anti-influenza activity against human influenza a viruses before viral adsorption, but not after viral adsorption, with 50% inhibitory concentration (ic50) values against a/pr/8/34 (h1n1) virus, a/aichi/2/68 (h3n2) virus and a/memphis/1/71 (h3n2) virus of 6.35+/-0.17, 2.84+/-1.98 and 0.53+/-0.10 microg/ml, respectively. the pl ...200818310920
molecular variability of hop stunt viroid: identification of a unique variant with a tandem 15-nucleotide repeat from naturally infected plum tree.for this study, 68 plum samples were collected from 12 provinces of china. low molecular weight rnas were extracted and used for dot-blot, reverse transcription-polymerase chain reaction (rt-pcr), return-polyacrylamide gel electrophoresis, and biological indexing using cucumber. results showed that 15 out of the 68 plum samples were positive for hop stunt viroid (hsvd). four positive samples were selected for cloning and sequence analysis. results indicated that most hsvd sequences from the plum ...200818324471
profiling of different bioactive compounds in functional drinks by high-performance liquid chromatography.in the present work, an hplc method is proposed to simultaneously detect and quantify water- and fat-soluble vitamins, phenolic compounds, carotenoids and chlorophylls in a single run, by using an ultradeactivated c18 column and gradient separation using trifluoroacetic acid, water and methanol. it is shown that the hplc method provides baseline separation of all these compounds with good resolution values in 40 min. moreover, other figures of merit of the method show a good linear response and ...200818325525
nondestructive determination of total soluble solid content and firmness in plums using near-infrared reflectance spectroscopy.the fruit industry requires rapid, economical, and nondestructive methods for classifying fruit by internal quality, which can be built into the processing line. total soluble solid content and firmness are the two indicators of plum internal quality that most affect consumer acceptance. these parameters are routinely evaluated using methods which involve destruction of the fruit; as a result, only control batches can be analyzed. the development of nondestructive analytical methods would enable ...200818363330
evaluation of metarhizium anisopliae (metsch) sorok. to target larvae and adults of capnodis tenebrionis (l.) (coleoptera: buprestidae) in soil and fiber band applications.the aim of this work has been to evaluate in the laboratory the potential of entomopathogenic fungi against adults and larvae of capnodis tenebrionis (l.) (coleoptera: buprestidae) through fiber band application and a potted plant bioassay with soil application, respectively. our previous findings revealed that metarhizium anisopliae eama 01/58-su isolate was the most virulent against neonate larvae of the buprestid. in the present work, m. anisopliae eama 01/58-su isolate has been also shown to ...200817961589
performance of mice in discrimination of liquor odors: behavioral evidence for olfactory attention.we examined performance of mice in discrimination of liquor odors by y-maze behavioral assays. thirsty mice were initially trained to choose the odor of a red wine in the y-maze. after successful training (>70% concordance for each trained mouse), the individual mice were able to discriminate the learned red wine from other liquors, including white wine, rosé wine, sake, and plum liqueur. however, when the mice were tested to distinguish fine differences between 2 brands of red wine, their perfo ...200818178544
identification of quantitative trait loci controlling symptom development during viral infection in arabidopsis thaliana.in compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. however, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. in a previous study on the arabidopsis thaliana-plum pox virus (ppv) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. in particular, cvi-1 (cape verde islands) accumulates viral ...200818184064
historical study of coma: looking back through medical and neurological texts.in the 1960s, two major works on coma by fisher, plum and poser were published and ushered in the beginning of a comprehensive clinical examination in coma. how these ideas matured has been rarely investigated. in this article, we describe observations and experiments that led to a better understanding of consciousness and coma in medical texts prior to that episode. we consulted medical texts published between 1640 and 1960. subject indexes and tables of contents of textbooks were reviewed for ...200818208847
[manifestation of malakoplakia in a urethral diverticulum in a female patient].we report about a rare case of malakoplakia in a female urethral diverticulum. a 25-year-old patient with a long history of recurrent urinary tract infections and a plum-sized, painful swelling on the vaginal roof presented for operative treatment. in the anamnesis the patient reported about two spontaneous perforations, emptying several millilitres of pus each time. after total operative excision using a vaginal approach the histology showed malakoplakia in a urethral diverticulum. we found the ...200818228192
specific and efficient cleavage of fusion proteins by recombinant plum pox virus nia protease.site-specific proteases are the most popular kind of enzymes for removing the fusion tags from fused target proteins. nuclear inclusion protein a (nia) proteases obtained from the family potyviridae have become promising due to their high activities and stringencies of sequences recognition. nia proteases from tobacco etch virus (tev) and tomato vein mottling virus (tvmv) have been shown to process recombinant proteins successfully in vitro. in this report, recombinant ppv (plum pox virus) nia p ...200818024078
lipid transfer proteins from rosaceae fruits share consensus epitopes responsible for their ige-binding cross-reactivity.four ige-binding epitopes have been characterized that cover a large area (40%) of the molecular surface of lipid transfer protein allergens of rosaceae (apple, peach, apricot, and plum). they mainly correspond to electropositively charged regions protruding on the molecular surface of the modeled apple (mal d 3), apricot (pru ar 3), and plum (pru d 3) allergens. two of these epitopes consist of consensus epitopes structurally conserved among the lipid transfer protein allergens from the rosacea ...200818036340
identification of plum pox virus pathogenicity determinants in herbaceous and woody hosts.plum pox virus (ppv) is a member of the genus potyvirus that is able to infect a large variety of plant species, including trees of the genus prunus, its natural host. when some ppv isolates are propagated for an extended time in herbaceous plants, their ability to infect trees is reduced. the molecular basis of this change in host infectivity is poorly understood. we report the construction of hybrid viruses from cdna clones of two d-strain isolates of ppv, ppv-d and ppv-r, which differ in thei ...200818052879
role of the 25-26 nt sirna in the resistance of transgenic prunus domestica graft inoculated with plum pox virus.the reaction of a genetically engineered plum clone (c5) resistant to plum pox virus (ppv) by graft inoculation with the virus was evaluated. the resistance in this clone has been demonstrated to be mediated through post-transcriptional gene silencing (ptgs). a single c5 plant out of 30 plants inoculated with ppv m strain by double chip-budding showed mild diffuse mosaic 'sharka' symptom at the bottom section of the scion. the upper leaves of this ppv-infected c5 plant remained symptomless and t ...200818071892
low genetic variability in the coat and movement proteins of american plum line pattern virus isolates from different geographic origins.until very recently isolates of american plum line pattern virus (aplpv) had not been reported from outside north america. the nucleotide sequences corresponding to the movement (mp) and coat (cp) proteins of eight aplpv isolates from five mediterranean countries were determined. sequence analysis showed that both mp and cp genes are highly conserved irrespective of geographic origin. the study of the distribution of synonymous and nonsynonymous changes along both open reading frames revealed th ...200818080796
determination of antioxidant activity and characterization of antioxidant phenolics in the plum vinegar extract of cherry blossom (prunus lannesiana).sakura-cha (salted cherry blossom tea) is a japanese tea that is traditionally served at celebrations such as wedding ceremonies. the production of sakura-cha includes the immersion of cherry blossom flowers in japanese plum vinegar, and through this process, the byproduct (plum vinegar extract of cherry blossom) is obtained. in this study, the antioxidant activity of the plum vinegar extract of cherry blossom was examined. the plum vinegar extract of cherry blossom had a greater superoxide anio ...200818092753
efficiency of removal of cadmium from aqueous solutions by plant leaves and the effects of interaction of combinations of leaves on their removal efficiency.removal of cadmium from aqueous solutions using 20 species of plant leaves and combinations of these leaves have been studied. several factors affecting the removal efficiency have been studied. the most efficient types of plant leaves for the removal of cadmium are those of styrax, plum, pomegranate and walnut. the interaction effect of the combined leaf samples on the efficiency of removal of cadmium has been found to be additive in combinations involving styrax plant leaves but seems to be an ...200817374434
assessment of the diversity and dynamics of plum pox virus and aphid populations in transgenic european plums under mediterranean conditions.the molecular variability of plum pox virus (ppv) populations was compared in transgenic european plums (prunus domestica l.) carrying the coat protein (cp) gene of ppv and non-transgenic plums in an experimental orchard in valencia, spain. a major objective of this study was to detect recombination between ppv cp transgene transcripts and infecting ppv rna. additionally, we assessed the number and species of ppv aphid vectors that visited transgenic and non-transgenic plum trees. test trees con ...200817605085
immunomodulatory properties of dietary plum on coccidiosis.the current study was conducted to evaluate the effect of dietary supplementation with a lyophilized powder made from plums (p) on host protective immune responses against avian coccidiosis, the most economically important parasitic disease of poultry. one-day-old white leghorn chickens were fed from the time of hatch with a standard diet either without p (control and p 0 groups) or supplemented with p at 0.5% (p 0.5) or 1.0% (p 1.0) of the diet. animals in the p 0, p 0.5, and p 1.0 groups were ...200817686519
differential induction of phenylalanine ammonia-lyase gene expression in response to in vitro callus unions of prunus spp.phenylalanine ammonia-lyase (pal) is a key enzyme in the synthesis of phenolic compounds, which play a prominent role in graft union formation, including the marked effects of their accumulation in incompatibility response. the purpose of this study was to assess changes in the abundance of pal mrna during graft union development. partial cdna clones encoding the enzyme were isolated from in vitro callus tissue in the apricot (prunus armeniaca l.) cultivar moniqui and the plum rootstock marianna ...200817910896
oligonucleotide microarray-based detection and genotyping of plum pox virus.plum pox virus (ppv) is the most damaging viral pathogen of stone fruits. the detection and identification of its strains are therefore of critical importance to plant quarantine and certification programs. existing methods to screen strains of ppv suffer from significant limitations such as the simultaneous detection and genotyping of several strains of ppv in samples infected with different isolates of the virus. a genomic strategy for ppv screening based on the viral nucleotide sequence was d ...200817920703
trans-specific s-rnase and sfb alleles in prunus self-incompatibility haplotypes.self-incompatibility in the genus prunus is controlled by two genes at the s-locus, s-rnase and sfb. both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. deduced polypeptide sequences of three myrobalan and three domestic plum s-rnases showed over 97% identity with s-rnases from other prunus species, including almond, sweet cherry, japanese apricot and japanese plum. the second intron, which is generally highly polymorphic bet ...200817989997
separation of dna for molecular markers analysis from leaves of the vitis vinifera.in the present study, three dna extraction procedures were examined to determine which might yield dna from grape leaves suitable for molecular analysis for rapd, ssr. aflp and etc analysis. the three methods examined were: the miniprep procedure and the modified ctab for difficult species and protocol ctab. only the modified ctab method consistently yielded dna suitable for polymerase chain reaction (pcr) amplification, regardless of plant growing conditions or leaf age. the quality and quantit ...200818817243
effect of plasticizer type and amount on hydroxypropyl methylcellulose-beeswax edible film properties and postharvest quality of coated plums (cv. angeleno).the effect of the composition of hydroxypropyl methylcellulose (hpmc)-beeswax (bw) edible coatings on stand-alone film properties and on postharvest quality of coated 'angeleno' plums was studied. glycerol (g) and mannitol (m) were tested as plasticizers at two different plasticizer/hpmc ratios (100:1 and 300:1 molar basis). bw content was 20 or 40% (dry basis). an increase in g content increased film flexibility and vapor permeability (wvp), whereas an increase in m content enhanced film brittl ...200818823125
analysis of cyanogens with resorcinol and picrate.the total cyanogenic potential of various substrates (flax seed, stones of peach, plum, nectarine and apricot as well as apple seeds, and various model compounds) was investigated by using the acid hydrolysis method, picrate method, and a novel method based on the reaction of cyanide liberated from plants with resorcinol and picrate. the hydrocyanic acid liberated from cyanogens was trapped by using a 1% sodium bicarbonate. then, 1 ml of extract was mixed with 1 ml of working reagent containing ...200818824068
odor-baited trap trees: a novel management tool for plum curculio (coleoptera: curculionidae).the plum curculio, conotrachelus nenuphar (herbst) (coleoptera: curculionidae), one of the most important pests of apple (malus spp.) in eastern and central north america, historically has been managed in new england apple orchards by three full block insecticide applications. efforts to reduce insecticide inputs against plum curculio include perimeter row sprays, particularly after petal fall, to control immigrating adults. the odor-baited trap tree approach represents a new reduced input strat ...200818767740
inhibitory effects of methanol extract of plum (prunus salicina l., cv. 'soldam') fruits against benzo(alpha)pyrene-induced toxicity in mice.this study was carried out to investigate the chemopreventive effects of immature plum extracts. the methanol extract of immature plums (plum 1), that are picked at 20-40 days before final harvest, has remarkably inhibited the growth of hepatoma hepg2 cells. the effects of immature plum extracts on hepatotoxicity in benzo(alpha)pyrene (b(alpha)p, carcinogen)-treated mice were investigated. male icr mice were pretreated with immature plum extracts (2.5 or 5 g/kg bw/day, for 5 days, i.p.) before t ...200818786596
host-specific effect of p1 exchange between two potyviruses.the potyviruses plum pox virus (ppv) and tobacco vein mottling virus (tvmv) have distinct host ranges and induce different symptoms in their common herbaceous hosts. to test the relevance of the p1 protein in host compatibility and pathogenicity, hybrid viruses were constructed in which the p1 coding sequence of ppv was completely or partially replaced by the corresponding sequences from tvmv. infections induced by these chimeric viruses revealed that the tvmv p1 and a ppv/tvmv hybrid p1 protein ...200818705848
influences of cross pollination on pollen tube growth and fruit set in zuili plums (prunus salicina).zuili plum (prunus salicina l.) trees usually set fruit poorly, although they produce high quality fruit. to elucidate the causes of the poor fruit set, pollen tube growth into pistils and fruit set percentage were investigated after cross-, self- and open-pollination. ovule development in zuili pistils was also investigated. pollen tube penetration into the ovules via the obturator and micropyle was best when zuili pistils were pollinated by cv. black amber (p. domestica) pollen grains, althoug ...200818713443
inhibition of growth and induction of differentiation of colon cancer cells by peach and plum phenolic compounds.the action of extracts from anthocyanin-enriched plums and peaches on growth and differentiation was studied with human colon cancer cells. growth inhibitory effects were observed in caco-2, sw1116, ht29 and ncm460 cells. in caco-2 cells but not in the other cells studied there was evidence for increased differentiation as judged by increased activity of alkaline phosphatase and dipeptidyl peptidase. a differentiating effect on caco-2 cells was not seen with cyanidin or cyanidin-3-glucoside but ...200818751377
the promiscuous evolutionary history of the family bromoviridae.recombination and segment reassortment are important contributors to the standing genetic variation of rna viruses and are often involved in the genesis of new, emerging viruses. this study explored the role played by these two processes in the evolutionary radiation of the plant virus family bromoviridae. the evolutionary history of this family has been explored previously using standard molecular phylogenetic methods, but incongruences have been found among the trees inferred from different ge ...200818559945
plum pox virus variability detected by the advanced analytical methods.plum pox virus (ppv) infects stone-fruit trees with important economical impact mainly in europe and mediterranean region. the data about ppv intra-species variability accumulated markedly in the last two decades. six ppv strains have been recognized using different approaches including serology, protein analysis, specific amplification, and genome sequencing. reliable and sensitive diagnostics is the most important requirement for application of early control and safety measures. therefore, man ...200818564894
the prehistory of potyviruses: their initial radiation was during the dawn of agriculture.potyviruses are found world wide, are spread by probing aphids and cause considerable crop damage. potyvirus is one of the two largest plant virus genera and contains about 15% of all named plant virus species. when and why did the potyviruses become so numerous? here we answer the first question and discuss the other.200818575612
antioxidant properties of dried plum ingredients in raw and precooked pork sausage.raw pork sausages with no antioxidant (control), 3% or 6% dried plum puree (dp), 3% or 6% dried plum and apple puree (dpa), or 0.02% butylated hydroxytoluene and butylated hydroxyanisole (bha/bht) were (1) stored raw in chubs at 4 degrees c (rr) and evaluated weekly over 28 d, (2) cooked as patties, vacuum packaged, and stored at 4 degrees c (pr) for weekly evaluation over 28 d, or (3) cooked, vacuum packaged, and stored at -20 degrees c (pf) and evaluated monthly over 90 d. dp at 3% or 6% level ...200818576997
altered gene expression changes in arabidopsis leaf tissues and protoplasts in response to plum pox virus infection.virus infection induces the activation and suppression of global gene expression in the host. profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. in this study, the arabidopsis affymetrix ath1 array was used to assess global gene expression changes in arabidopsis thaliana plants infected with plum pox virus (ppv). to identify early genes in response to ppv infection, an arab ...200818613973
a rapid and effective method for rna extraction from different tissues of grapevine and other woody plants.rna quality and integrity are critical for many studies in plant molecular biology. high-quality rna extraction from grapevine and other woody plants is problematic due to the presence of polysaccharides, polyphenolics and other compounds that bind or co-precipitate with the rna.200818618437
addition of fructooligosaccharides and dried plum to soy-based diets reverses bone loss in the ovariectomized rat.dietary bioactive components that play a role in improving skeletal health have received considerable attention in complementary and alternative medicine practices as a result of their increased efficacy to combat chronic diseases. the objectives of this study were to evaluate the additive or synergistic effects of dried plum and fructooligosaccharides (fos) and to determine whether dried plum and fos or their combination in a soy protein-based diet can restore bone mass in ovarian hormone defic ...200818955356
scale insects and mealy bugs (homoptera: coccoidea) attacking deciduous fruit trees in the western north coast of alexandria, egypt.this investigation covered a survey of scale insects and mealy bugs infesting ten growing species of deciduous fruit trees in three localities in alexandria govemorate. these localities were merghem, burg el-arab, and el-nahda about 50 km. west of alexandria under both rain-fed and irrigation system conditions. the common inspected fruit trees were fig, white mulberry, pomegranate, apple, pear, apricot, european plum, peach, almond, and persimmon. it was shown that a group of twenty scale insect ...200819226793
[light competition and productivity of agroforestry system in loess area of weibei in shaanxi].agroforestry is the most effective way for the restoration of disturbed land on loess plateau and the development of poorly local economy. taking the tree-based intercropping systems of walnut or plum with soybean or pepper in the loess area of weibei as test objects, the photosynthesis, growth, and yield of soybean (qindou 8) and pepper (shanjiao 981) in the systems were studied. the results showed that the photosynthetic active radiation (par), net photosynthetic rate (pn), growth, and yield o ...200819238840
[measurement of fruit maturity based on laser-induced photoluminescence spectrum].grounding on the concepts of biophotonics measurement, the authors first used a red semiconductor laser (655 nm) to irradiate fruits. compared with other kinds of illuminating sources, the red semiconductor laser is less expensive and takes little space. the laser-induced photoluminescence spectrums could be detected by coupling fibre-optics probe when the fruits are illuminated by laser. and the spectrum has a distinct peak of relative intensity around the 685 nm wavelength that varies with the ...200819248480
quantitative resistance traits and suitability of woody plant species for a polyphagous scarab, popillia japonica newman.the japanese beetle, popillia japonica newman, has an unusually broad host range among deciduous woody plants, yet it feeds only sparingly, or not at all, on certain species in the field. we evaluated beetles' preference, survival over time and fecundity on eight woody plant species historically rated as susceptible or resistant and, after verifying those ratings, tested whether resistance is correlated with so-called quantitative defense traits including leaf toughness, low nutrient content (wa ...200819161699
abiotic factors and trap design modulate the performance of traps used to monitor the plum curculio.all published studies on effects of abiotic factors on plum curculio, conotrachelus nenuphar (hersbt), adults have taken a retrospective approach. here, we present the results of experiments where factors and their levels were determined and controlled a priori. we compared the effectiveness of miniature pyramidal traps (45 by 20 by 20 cm) constructed of four kind of materials--wood, geotextile, nylon screening, and corrugated plastic--to monitor overwintered and summer adults of univoltine plum ...200819133465
trace element levels in some dried fruit samples from turkey.trace element levels in nine different dried fruit samples from kayseri, turkey were determined by flame atomic absorption spectrometry after dry and wet digestion methods. good accuracy was assured by the analysis of standard reference material (nist-srm 1515-apple leaves). the contents of trace elements in the samples were determined in the ranges of 1.68-4.52, 0.6-9.4, 5.5-12.4, 6.76-64.1, 4.74-25.5, 0.2-1.78, 0.8-6.17 and 0.1-0.81 microg/g for copper, nickel, lead, iron, manganese, cobalt, c ...200818991103
evaluation of reproductive compatibility of interstrain matings among plum curculio populations in the eastern united states.reproductive compatibility of interstrain matings in plum curculio, conotrachelus nenuphar (herbst), was studied during the summers of 2004 and 2006. there was a unidirectional incompatibility in the cross-matings along a rough north to south transect of the range of plum curculio (4 x 4 two-factorial design). there was significantly lower fertility in west virginia males mated with new york and virginia females (40 and 29%, respectively), and florida males had a significantly lower fertility wi ...200819036199
potential of two entomopathogenic nematodes for suppression of plum curculio (conotrachelus nenuphar, coleoptera: curculionidae) life stages in northern climates.plum curculio, conotrachelus nenuphar, is a major pest of pome and stone fruits in north america. we evaluated the potential efficacy of two entomopathogenic nematode species for suppression of plum curculio in northern regions, targeting life stages that reside in soil during spring and summer. a strain of heterorhabditis bacteriophora isolated from soil infested with plum curculio in northern utah and a commercially available strain of steinernema feltiae known to tolerate cool temperatures we ...200819036207
shining light on foreign animal diseases: the story of plum island. 200819069040
[combination of acupuncture with cupping increases life quality of patients of osteoporosis].to observe therapeutic effect of "general regulation" acupuncture and cupping therapy on osteoporosis.200819127911
novel paraconiothyrium species on stone fruit trees and other woody hosts.coniothyrium-like fungi are common wood and soil inhabitants and hyperparasites on other fungi. they belong to different fungal genera within the pleosporales. several isolates were obtained on wood of different prunus species (plum, peach and nectarine) from south africa, on actinidia species from italy and on laurus nobilis from turkey. morphological and cultural characteristics as well as dna sequence data (5.8s nrdna, its1, its2, partial ssu nrdna) were used to characterise them. the isolate ...200820467483
novel phaeoacremonium species associated with necrotic wood of prunus trees.the genus phaeoacremonium is associated with opportunistic human infections, as well as stunted growth and die-back of various woody hosts, especially grapevines. in this study, phaeoacremonium species were isolated from necrotic woody tissue of prunus spp. (plum, peach, nectarine and apricot) from different stone fruit growing areas in south africa. morphological and cultural characteristics as well as dna sequence data (5.8s rdna, its1, its2, beta-tubulin, actin and 18s rdna) were used to iden ...200820467488
optimal timing of mr sialography by use of a simple method of stimulating the salivary gland: a preliminary report.the present study was performed for determining the optimal timing of mr sialography by use of the japanese pickled plum (umeboshi) for promoting secretion by the salivary glands. mr sialography was performed in four healthy male volunteers. the four volunteers were examined before and 10 min after stimulation with umeboshi. on the next examination, three volunteers were examined before and after umeboshi stimulation every 1 min up to 5 min to allow assessment of the temporal changes in duct vis ...200820821149
antioxidant properties of plum concentrates and powder in precooked roast beef to reduce lipid oxidation.boneless beef roasts (semimembranosus+adductor) were injected (20%) with a brine containing (1) no plum ingredient (control), (2) 2.5 or 5% fresh plum juice concentrate (fp), (3) 2.5 or 5% dried plum juice concentrate (dp), or (4) 2.5 or 5% spray dried plum powder (pp). whole roasts were cooked, vacuum-packaged and stored at <4.0°c for 10wk. at 2wk intervals, evaluations were performed on sliced product to determine vacuum-packaged purge, allo-kramer shear force, lipid oxidation (tbars), color s ...200822063828
phylogenetic relationships of xylella fastidiosa strains isolated from landscape ornamentals in southern california.abstract xylella fastidiosa is an insect-borne, xylem-limited pathogenic bacterium that has been associated with a rise in incidence of diseased landscape ornamentals in southern california. the objective of this study was to genetically characterize strains isolated from ornamental hosts to understand their distribution and identity. strains of x. fastidiosa isolated from ornamentals were characterized using a multiprimer polymerase chain reaction (pcr) system, random amplified polymorphic dna ...200718943935
no recombination detected in artificial potyvirus mixed infections and between potyvirus derived transgenes and heterologous challenging potyviruses.risk-assessment studies of virus-resistant transgenic plants (vrtps) focussing on recombination of a plant virus with a transgenic sequence of a different virus should include a comparison of recombination frequencies between viruses in double-infected non-transgenic plants with those observed in singly infected transgenic plants to estimate recombination incidence in vrtps. in this study, the occurrence of recombination events was investigated in non-transgenic plants double-infected with two d ...200718001687
chain of demand. a host of former triad execs are landing plum jobs in the wake of company's sale to community--who's next in line?community health system's $6.97 billion acquisition of competitor triad hospitals this past summer left many former triad executives wealthy but out of work. some are enjoying a respite while others are being plucked up by chs competitors. by letting so many triad executives go, according to management guru tim vogus, right, chs sent a clear message that its newly acquired business will be run its way.200718020042
gas chromatographic-mass spectrometric characterisation of plant gums in samples from painted works of art.this paper presents an analytical gc-ms procedure to study the chemical composition of plant gums, determining aldoses and uronic acids in one step. the procedure is based on the silylation of aldoses and uronic acids, released from plant gums by microwave assisted hydrolysis, and previously converted into the corresponding diethyl-dithioacetals and diethyl-dithioacetal lactones. using this method only one peak for each compound is obtained, thus providing simple and highly reproducible chromato ...200718023451
[skull plasty to correct congenital craniosynostosis].to investigate the technique of skull reconstruction for the congenital craniosynostosis.200717926845
effect of dips in a 1-methylcyclopropene-generating solution on 'harrow sun' plums stored under different temperature regimes.the effect of postharvest dips in a 1-methylcyclopropene-generating solution of the formulation afxrd-038 (rohm & haas) on plum fruit (prunus salicina lindell cv. 'harrow sun') quality and ripening during storage was determined. fruit weight loss, tissue firmness, soluble solids content (ssc), titratable acidity (ta), ethylene production, respiration, and the activities of the cell wall modifying enzymes polygalacturonase (pg), 1,4-beta-d-glucanase/glucosidase (egase), beta-galactosidase (beta-g ...200717663566
reduced reproductive function in wild baboons (papio hamadryas anubis) related to natural consumption of the african black plum (vitex doniana).several authors have suggested that the consumption of plant compounds may have direct effects on wild primate reproductive biology, but no studies have presented physiological evidence of such effects. here, for two troops of olive baboons (papio hamadryas anubis) at gashaka-gumti national park, nigeria, we show major seasonal increases in levels of fecal progesterone metabolites in females, and provide evidence that this is linked to the consumption of natural plant compounds. increases in fec ...200717681506
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