Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| detection of plant dna in the bronchoalveolar lavage of patients with ventilator-associated pneumonia. | hospital-acquired infections such as nosocomial pneumonia are a serious cause of mortality for hospitalized patients, especially for those admitted to intensive care units (icus). despite the number of the studies reported to date, the causative agents of pneumonia are not completely known. herein, we found by molecular technique that vegetable and tobacco dna may be detected in the bronchoalveolar lavage from patients with ventilator-associated pneumonia (vap). | 2010 | 20585574 |
| rapid detection of squash leaf curl virus by loop-mediated isothermal amplification. | a loop-mediated isothermal amplification (lamp) assay was employed to develop a simple and efficient system for the detection of squash leaf curl virus (slcv) in diseased plants of squash (cucurbita pepo) and melon (cucumis melo). completion of lamp assay required 30-60 min under isothermal conditions at 65 degrees c by employing a set of four primers targeting slcv. although the sensitivity of the lamp assay and the polymerase chain reaction (pcr) assay was comparable at high virus concentratio ... | 2010 | 20603151 |
| protective role of three vegetable peels in alloxan induced diabetes mellitus in male mice. | the hitherto unknown glucose regulating role of three vegetable peels from cucurbitaceae family was evaluated. in a preliminary study, effects of ethanolic extracts of cucurbita pepo, cucumis sativus and praecitrullus fistulosus peels were studied at 250 and 500 mg kg(-1) d(-1) for 15 days in the alterations in serum glucose and in hepatic lipid peroxidation (lpo) in male mice. in the pilot experiment, the effective and safe concentration of each peel was administered (p.o.) for 10 consecutive d ... | 2010 | 20614191 |
| cytotoxicity of hydro-alcoholic extracts of cucurbitapepo and solanum nigrum on hepg2 and ct26 cancer cell lines. | plants are used worldwide for the treatment of diseases, and novel drugs continue to be developed through research from plants. there are more than 20,000 species of plants used in traditional medicines, and these are all potential reservoirs for new drugs. cucurbitapepo has been used in traditional folk medicine to treat cold and alleviate ache. previous pharmacological tests have shown that it possesses antiviral, anti-inflammatory, and analgesic effects. also, solanum nigrum has been used as ... | 2010 | 20931075 |
| suppressive effect of pectic polysaccharides from cucurbita pepo l. var. styriaca on citric acid-induced cough reflex in guinea pigs. | several water-soluble pectic polysaccharides were isolated from the pumpkin fruit biomass and characterized by composition, structural features and molecular properties. the pectic polysaccharides were tested for antitussive activity by studying the effects of citric acid-induced cough reflex in guinea pigs and reactivity of the airway smooth muscle in vivo conditions in comparison to the narcotic drug codeine. oral administration of all pectic polysaccharides from pumpkin inhibited the number o ... | 2010 | 21062638 |
| influence of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. | three studies were conducted to determine the effect of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. in all three studies, samples of wireworm populations were taken from the soil by using oat, avena sativa l., baits. treatments were defined by the previous year's crop and were chosen to reflect common crop rotations in the region. across all three studies, eight wireworm species were recovered from the baits: conoderus amplicollis (gyllen ... | 2010 | 21309229 |
| effects of cucumber mosaic virus infection on vector and non-vector herbivores of squash. | plant chemicals mediating interactions with insect herbivores seem a likely target for manipulation by insectvectored plant pathogens. yet, little is currently known about the chemical ecology of insect-vectored diseases or their effects on the ecology of vector and nonvector insects. we recently reported that a widespread plant pathogen, cucumber mosaic virus (cmv), greatly reduces the quality of host-plants (squash) for aphid vectors, but that aphids are nevertheless attracted to the odors of ... | 2010 | 21331245 |
| [an extracellular ph changes registration by confocal microscopy in higher plant at the excitation potentials generation]. | confocal microscopy technique was applied for registration of apoplast ph changes in the cucurbita pepo seedling stem during generation of action potential and variation potential. fluorescent dye fitc-dextran was used for ph-changes registration. analysis of fluorescence images and fluorescence spectra showed, that fitc-dextran was localized in cell walls. propagation of action potential and variation potential was accompanied by transient increase of fluorescence intensity, which indicated alk ... | 2010 | 20799619 |
| inheritance of p,p'-dde phytoextraction ability in hybridized cucurbita pepo cultivars. | cucurbita pepo ssp pepo (zucchini) has been shown to uniquely phytoextract percent level amounts of dichlorodiphenyldichloroethylene (dde) and other organic contaminants from soil. since c. pepo ssp ovifera (squash) does not have this ability, a three-year field trial was conducted to follow the inheritance pattern of dde accumulation for cross pollinated c. pepo cultivars. parental zucchini and squash cultivars (3 each) had stem-to-soil bioconcentration factors (bcf, contaminant ratio of stem t ... | 2010 | 20507162 |
| understanding the physiological and molecular mechanism of persistent organic pollutant uptake and detoxification in cucurbit species (zucchini and squash). | cucurbita pepo ssp pepo (zucchini) roots phytoextract significant amounts of persistent organic pollutants (pops) from soil, followed by effective translocation to aboveground tissues. the closely related c. pepo ssp ovifera (squash) does not have this ability. in a dde-contaminated field soil, zucchini roots and stems contained 3.6 and 6.6-fold greater contaminant than did squash tissues, respectively, and zucchini phytoextracted 12-times more dde from soil than squash. in batch hydroponics, sq ... | 2010 | 20507062 |
| bryonolic acid: a large-scale isolation and evaluation of heme oxygenase 1 expression in activated macrophages. | bryonolic acid (ba) is a triterpenoid found in the cucurbitaceae family of plants. our interests in the immunomodulatory effects of this class of natural products led us to discover that ba induces a marked increase in the expression of a phase 2 response enzyme, heme oxygenase 1 (ho-1), in a dose-dependent manner. this phenotype has translational implications in malarial disease progression, and consequently we developed a large-scale isolation method for ba that will enable future in vitro and ... | 2010 | 20481554 |
| mobility and half-life of bensulide in agricultural soil. | environmentally and economically viable agriculture requires the use of cultivation practices that maximize agrochemical efficacy while minimizing their off-site movement. bensulide [o, o-diisopropyl s-2-phenylsulfonylaminoethyl phosphorodithioate] is one of the few herbicides from the organophosphate group used for control of weeds that threaten numerous crops. a field study was conducted on a silty-loam soil of 10% slope at kentucky state university research farm to monitor off-site movement a ... | 2010 | 20390925 |
| cloning and characterisation of two ctr1-like genes in cucurbita pepo: regulation of their expression during male and female flower development. | ethylene is an essential regulator of flower development in cucurbita pepo, controlling the sexual expression, and the differentiation and maturation of floral organs. to study the action mechanism of ethylene during the male and female flower development, we have identified two ctr1 homologues from c. pepo, cpctr1 and cpctr2, and analysed their expressions during female and male flower development and in response to external treatments with ethylene. cpctr1 and cpctr2 share a high homology with ... | 2010 | 20390430 |
| a biosensor based on zucchini (cucurbita pepo l.) homogenate as a biorecognition layer for ascorbic acid determination. | an amperometric biosensor based on zucchini (cucurbita pepo) tissue homogenate is presented. the zucchini tissue homogenate was crosslinked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. the zucchini tissue contained the enzyme ascorbate oxidase and this enzyme catalyzed the oxidation of ascorbic acid in the presence of dissolved oxygen. the principle of the measurements was based on the determination of the decrease in the dissolved oxygen level. determinations we ... | 2010 | 20384439 |
| [effects of exogenous ca2+ on morphological and photosynthetic characteristics and chlorophyll fluorescent parameters of squash seedlings under high temperature and strong light stress]. | taking squash (cucurbita pepo l.) variety alan as test object, this paper studied the effects of exogenous ca2+ on the morphological and photosynthetic characteristics and chlorophyll fluorescent parameters of squash seedlings under the cross-stress of high temperature and strong light. under the stress, applying 5-20 mmol x l(-1) of ca2+ increased the plant height, leaf area, chlorophyll and carotenoid contents, photosynthetic rate (pn), stoma conductance (gs), transpiration rate (tr), maximal ... | 2010 | 21361006 |
| cadmium induced changes in subcellular glutathione contents within glandular trichomes of cucurbita pepo l. | plants cope with cadmium (cd) stress by complexation with phytochelatins (pc), metallothioneins and glutathione and sequestration within vacuoles. especially glutathione was found to play a major role in cd detoxification as cd shows a high binding affinity towards thiols and as glutathione is a precursor for pc synthesis. in the present study, we have used an immunohistochemical approach combined with computer-supported transmission electron microscopy in order to measure changes in the subcell ... | 2010 | 19424775 |
| effects of amendments on the uptake and distribution of ddt in cucurbita pepo ssp pepo plants. | the effects of soil amendments on the phytoextraction of summation operatorddt (ddt + ddd + dde) from soil ([ summation operatorddt] approximately 1500 ng/g) by a pumpkin variety of cucurbita pepo ssp pepo were tested and the patterns of summation operatorddt storage throughout the plant shoot were examined. the soil amendments did not increase the total amount of summation operatorddt extracted into plant shoots, but new information about summation operatorddt distribution in the plants was obt ... | 2010 | 19762136 |
| the effects of planting methods and head pruning on seed yield and yield components of medicinal pumpkin (cucurbita pepo subsp. pepo convar. pepo var. styriaca) at low temperature areas. | this experiment carried out to evaluate the effects of planting methods (seed sowing and transplanting) and head pruning (no pruning, pruning after 12th node and pruning after 16th node) on yield and yield components such as number of branches (sub-branches) per plant, fruits per plant, growth, fruit size, weight of fresh fruit, weight of seeds per fruit, number of seeds per fruit and seed yield of medicinal pumpkin. the experiment was carried out based of factorial experiment with randomized co ... | 2009 | 19580007 |
| enhancing the release and plant uptake of pahs with a water-soluble purine alkaloid. | the effect of a common plant alkaloid, caffeine, on the release and plant uptake of some polycyclic aromatic hydrocarbons (pahs) in soils was investigated. cucurbita pepo (ssp. pepo cv. gold rush) was grown in pah-spiked media in the presence and absence of caffeine. solubility tests initially confirmed the ability of caffeine to dissolve pahs mixtures of anthracene, phenanthrene, pyrene, benzo[a]pyrene and benzo[ghi]perylene. extraction experiments also highlighted its potential as a pah-releas ... | 2009 | 19477480 |
| plant mitochondria electron partitioning is independent of short-term temperature changes. | we tested the hypotheses that relative activity of the less efficient alternative oxidase (aox) path changes with diurnal temperature changes, and thus changes carbon use efficiency with temperature. the activities of the alternative and cytochrome oxidase (cox) paths in plant tissues of three species were determined by measuring 18o/16o discrimination and total respiration from 17 to 36 degrees c. a new, more accurate method for calculating oxygen uptake rate from the mass spectrometry data was ... | 2009 | 19210639 |
| genes for "reverse" fruit striping in squash (cucurbita pepo). | longitudinal striping is a common fruit color pattern in cucurbita pepo and is conferred by any of several alleles at the light coloration-1 locus. normally, dark stripes appear over the fruit surface areas between the 10 main subepidermal vein tracts with the lighter background color over and adjacent to these vein tracts. less commonly, the striping is "reversed," that is, lighter than the background color. the present work was conducted to elucidate the mode of inheritance of reverse striping ... | 2009 | 19153086 |
| leaf herbivory increases floral fragrance in male but not female cucurbita pepo subsp. texana (cucurbitaceae) flowers. | mutualisms are key interactions that affect population dynamics and structure communities, but the extent to which mutualists can attract potential partners may depend on community context. many studies have shown that leaf herbivory reduces pollinator visitation and have focused on reduced floral visual display and rewards as potential mechanisms. however, olfactory display plays a critical role in mediating interactions between plants, herbivores, and pollinators. we simulated leaf damage in c ... | 2009 | 21628242 |
| influence of cover crop and intercrop systems on bemisia argentifolli (hemiptera: aleyrodidae) infestation and associated squash silverleaf disorder in zucchini. | field experiments were conducted to evaluate the effects of cover cropping and intercropping on population densities of silverleaf whitefly, bemisia argentifolli bellow and perring, and the incidence of squash silverleaf disorder (ssl) in zucchini, cucurbita pepo l., in oahu, hi. two cover crops, buckwheat (bw), fagopyrum esculentum moench, and white clover (wc), trifolium repens l., or sunn hemp (sh), crotolaria juncea l., and an intercropped vegetable, okra, abelmonchus esculentus l., were eva ... | 2009 | 19389294 |
| nanoparticle penetration and transport in living pumpkin plants: in situ subcellular identification. | in recent years, the application of nanotechnology in several fields of bioscience and biomedicine has been studied. the use of nanoparticles for the targeted delivery of substances has been given special attention and is of particular interest in the treatment of plant diseases. in this work both the penetration and the movement of iron-carbon nanoparticles in plant cells have been analyzed in living plants of cucurbita pepo. | 2009 | 19389253 |
| initial formation of an indigenous crop complex in eastern north america at 3800 b.p. | although geneticists and archaeologists continue to make progress world-wide in documenting the time and place of the initial domestication of a growing number of plants and animals, far less is known regarding the critically important context of coalescence of various species into distinctive sets or complexes of domesticates in each of the world's 10 or more independent centers of agricultural origin. in this article, the initial emergence of a crop complex is described for one of the best-doc ... | 2009 | 19366669 |
| ultraweak and induced photon emission after wounding of plants. | the ultraweak and induced photon emission were measured by a single photon counting equipment (photomultiplier hamamatsu r562) on cucurbita pepo variaca styriacae after wounding. wounding significantly changes the emission from a stationary to a nonstationary state and the shape of the decay curve obtained after light illumination. the rise in the ultraweak photon emission depends on the kind of wounding and its localization on the plant. the decay curves obtained after wounding could be better ... | 2009 | 19254235 |
| cucurbitane and hexanorcucurbitane glycosides from the fruits of cucurbita pepo cv dayangua. | phytochemical investigation of the fruits of cucurbita pepo cv dayangua has led to the isolation of two cucurbitane glycosides: cucurbitacin l 2-o-beta-d-glucopyranoside (1), cucurbitacin k 2-o-beta-d-glucopyranoside (2) and two hexanorcucurbitane glycosides: 2,16-dihydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 2-o-beta-d-glucopyranoside (3) and 16-hydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 3-o-alpha-l-rhamnopyranosyl-(1 --> 2)-beta-d-glucopyranoside (4). compounds 1 ... | 2009 | 17885839 |
| [investigation of salt-soluble proteins of pumpkin seeds (cucurbita pepo l.) by a column gradient extraction method]. | 2009 | 5870889 | |
| assay-dependent phytotoxicity of nanoparticles to plants. | the effects of five nanomaterials (multiwalled carbon nanotubes [mwcnts], ag, cu, zno, si) and their corresponding bulk counterparts on seed germination, root elongation, and biomass of cucurbita pepo (zucchini) were investigated. the plants were grown in hydroponic solutions amended with nanoparticles or bulk material suspensions at 1000 mg/l. seed germination was unaffected by any of the treatments, but cu nanoparticles reduced emerging root length by 77% and 64% relative to unamended controls ... | 2009 | 19924897 |
| spent mushroom substrates as component of growing media for germination and growth of horticultural plants. | this research work was conducted in order to investigate the possibility of using spent mushroom substrate (sms) in the production of horticultural seedlings replacing part of the peat in the growing media. three vegetable species with different salt sensitivities, the less sensitive being tomato (lycopersicon esculentum var. muchamiel), the moderately salt-sensitive being courgette (cucurbita pepo l. var. afrodite f1) and the most salt-sensitive being pepper (capsicum annum l. var. lamuyo f1) w ... | 2009 | 19409775 |
| rapid diagnosis of plant virus diseases by transmission electron microscopy. | a clear and rapid diagnosis of plant virus diseases is of great importance for agriculture and scientific experiments in plant phytopathology. even though negative staining and transmission electron microscopy (tem) are often used for detection and identification of viral particles and provide rapid and reliable results, it is necessary to examine ultrastructural changes induced by viruses for clear identification of the disease. with conventional sample preparation for tem it can take several d ... | 2009 | 19665483 |
| risk assessment of genetically engineered crops: fitness effects of virus-resistance transgenes in wild cucurbita pepo. | the development of crops genetically engineered for pathogen resistance has raised concerns that crop-to-wild gene flow could release wild or weedy relatives from regulation by the pathogens targeted by the transgenes that confer resistance. investigation of these risks has also raised questions about the impact of gene flow from conventional crops into wild plant populations. viruses in natural plant populations can play important roles in plant fecundity and competitive interactions. here, we ... | 2009 | 19688918 |
| species and genus specificity of the intergenic spacer (igs) in the ribosomal rna genes of cucurbitaceae. | the use of intergenic spacer (igs) fragments of plant ribosomal dna (rdna) for the differentiation between genera and species is tested by cross-hybridization experiments with different igs probes of two cucurbitaceae, cucurbita pepo (zucchini) and cucumis sativus (cucumber). hybridization with cloned fragments of different parts of the igs of ribosomal dna exhibit a different degree of conservation within and between the cucurbitaceae genera. in general, cucurbita species seem to be closer rela ... | 2009 | 2631730 |
| [resistance of squash genotypes to bemisia tabaci (genn.) biotype b (hemiptera: aleyrodidae)]. | field assays were performed to evaluate the attractiveness and the non-preference of whitefly bemisia tabaci (genn.) biotype b for oviposition on squash genotypes (cucurbita pepo) and to observe the susceptibility of genotypes (novita, sandy, caserta cac melhorada, novita plus, samira, bianca, af-2858 and caserta ts) to silverleaf symptoms. the sandy genotype was the least attractive to whitefly, while novita plus, af-2858 and samira were the most attractive. the caserta cac melhorada genotype w ... | 2009 | 19768274 |
| wild bee abundance and pollination service in cultivated pumpkins: farm management, nesting behavior and landscape effects. | recent declines in managed and feral honey bee populations have greatly increased interest in the current and potential role of wild pollinators in agricultural pollination. pumpkin (cucurbita pepo l.) has great potential to be served by wild pollinators because of a reliable and widespread group of bee species that are commonly associated with their flowers, including bumble bees (bombus spp.), and, in the americas, two genera of specialist ground nesting bees (peponapis and xenoglossa). we exa ... | 2009 | 19449636 |
| computer analysis of phytochrome sequences from five species: implications for the mechanism of action. | the amino acid sequences of phytochrome from avena sativa, oryza sativa, curcurbita pepo, pisum sativum and arabidopsis thaliana have been analyzed with a variety of computer programs, with a view to identifying areas of the protein which contribute to the properties of this photoreceptor. a region at the c-terminus has been shown to be amphiphilic, and by analogy with surface-seeking peptides, may be responsible for interaction of phytochrome with lipid bilayers. possible targeting sequences in ... | 2009 | 2291773 |
| effect of home freezing and italian style of cooking on antioxidant activity of edible vegetables. | in this study, we analyzed the modifications of antioxidant activity consequent to 3 typical home cooking practices (steaming, boiling, and microwave cooking) in fresh and home frozen vegetables. six different vegetable species were examined: carrots (daucus carota l.), zucchini (cucurbita pepo l.), tomatoes (solanumn lycopersicum l.), green beans (phaseolus vulgaris l.), peas (pisum sativum l.), and yellow peppers (capsicum annuum l.). all vegetables were conventional products and were analyzed ... | 2008 | 19241586 |
| hplc-dad-ms(n) characterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity. | carotenoids including carotenoid esters from six apricot (prunus armeniaca l.) cultivars and from eight cultivars from three pumpkin species (cucurbita maxima duch., cucurbita pepo l., and cucurbita moschata duch.) were extracted without saponification, separated on a c-30 reversed-phase column and characterised by high-performance liquid chromatography/atmospheric pressure chemical ionisation-mass spectrometry (lc-ms). the predominant free carotenoids were quantified by hplc with diode array de ... | 2008 | 26049248 |
| ssr-based genetic linkage map of cucurbita moschata and its synteny with cucurbita pepo. | the first ssr-based genetic linkage map of cucurbita moschata was created by integrating the maps of two f2 populations with one common parent developed from the crosses waltham butternut (wb) x nigerian local (nl) and zhou (a hull-less type) x wb. the integrated c. moschata map comprises 205 ssr markers and two morphological traits (gr and n). the map is composed of 27 linkage groups with a marker density of 7 cm. comparing the c. moschata map with the published cucurbita pepo map, we found a h ... | 2008 | 18956020 |
| effect of ripe fruit pulp extract of cucurbita pepo linn. in aspirin induced gastric and duodenal ulcer in rats. | a significant decrease in alkaline phosphatase (ap) activity and mucosal thickness and increase in ulcer index (ui) was observed in aspirin treated stomach and duodenum of albino rats. however, pretreatment with c. pepo fruit pulp extract for 14 consecutive days showed increase in ap activity and mucosal thickness along with decrease in ui, suggesting gastroduodenal protective and anti-ulcerogenic properties of c. pepo. | 2008 | 18949893 |
| population size and incidence of virus infection in free-living populations of cucurbita pepo. | impact assessments of virus resistance transgene introgression into wild, free-living populations are important for determining whether these transgenes present a risk to agriculture or the environment. transgenic virus-resistant cucurbita pepo ssp. ovifera var. ovifera l. (squash) cultivars have been commercialized, and may be cultivated in close proximity to cross-compatible wild, free-living relatives (c. pepo subsp. pepo vars. ozarkana and texana). therefore, the potential impact of these vi ... | 2008 | 19081007 |
| cold tolerance and plant growth promotion potential of serratia marcescens strain srm (mtcc 8708) isolated from flowers of summer squash (cucurbita pepo). | to determine the cold tolerance and plant growth promotion potential of serratia marcescens strain srm (mtcc 8708). | 2008 | 18028329 |
| dde remediation and degradation. | ddt and its metabolites, ddd and dde, have been shown to be recalcitrant to degradation. the parent compound, ddt, was used extensively worldwide starting in 1939 and was banned in the united states in 1973. the daughter compound, dde, may result from aerobic degradation, abiotic dehydrochlorination, or photochemical decomposition. dde has also occurred as a contaminant in commercial-grade ddt. the p,p'-dde isomer is more biologically active than the o,p-dde, with a reported half-life of -5.7 ye ... | 2008 | 18069646 |
| purine-containing cucurbitane triterpenoids from cucurbita pepo cv dayangua. | phytochemical investigation of the fruits of cucurbita pepo cv dayangua led to the isolation of cucurbitaglycosides a (1) and b (2). this is the first report of cucurbitane triterpenoids with a purine unit. their structures were elucidated mainly based on interpretation of hresims results, as well as 1d and 2d nmr spectra. cucurbitaglycosides a and b showed cytotoxic activity against the human epithelial carcinoma cell line hela with ic50 of 17.2 and 28.4 microg/ml, respectively. | 2008 | 18325551 |
| microsatellites for the genus cucurbita and an ssr-based genetic linkage map of cucurbita pepo l. | until recently, only a few microsatellites have been available for cucurbita, thus their development is highly desirable. the austrian oil-pumpkin variety gleisdorfer olkürbis (c. pepo subsp. pepo) and the c. moschata cultivar soler (puerto rico) were used for ssr development. ssr-enriched partial genomic libraries were established and 2,400 clones were sequenced. of these 1,058 (44%) contained an ssr at least four repeats long. primers were designed for 532 ssrs; 500 primer pairs produced fragm ... | 2008 | 18379753 |
| sunflower (helianthus annuus l.) as a pre-columbian domesticate in mexico. | mexico has long been recognized as one of the world's cradles of domestication with evidence for squash (cucurbita pepo) cultivation appearing as early as 8,000 cal b.c. followed by many other plants, such as maize (zea mays), peppers (capsicum annuum), common beans (phaseolus vulgaris), and cotton (gossypium hirsutum). we present archaeological, linguistic, ethnographic, and ethnohistoric data demonstrating that sunflower (helianthus annuus) had entered the repertoire of mexican domesticates by ... | 2008 | 18443289 |
| pancreatic proteolytic enzymes of ostrich purified on immobilized protein inhibitors. characterization of a new form of chymotrypsin (chtr1). | four forms of chymotrypsin (chtr1, chtr2, chtr3, chtr4), one form of trypsin and one form of elastase were purified from a slightly alkaline extract of ostrich (struthio camelus) pancreas. the zymogens in the crude extract were activated with immobilized trypsin and then separated by affinity chromatography using immobilized inhibitors and ion exchange chromatography. one of the purified forms of chymotrypsin (chtr1) exhibited an unusual interaction with the highly selective protein trypsin inhi ... | 2008 | 18598777 |
| influence of compost amendment on pyrene availability from artificially spiked soil to two subspecies of cucurbita pepo. | the dissolved organic matter (dom) fraction of soil organic matter (som) may positively contribute to polycyclic aromatic hydrocarbons (pahs) bioavailability. this work investigated the effects of dom-rich and pahs-free compost amendment on the plant uptake of pyrene. two subspecies of cucurbita pepo (ssp. pepo cv. raven and ssp. texana cv. sunray) were grown for three weeks in a spiked soil containing 83.9 mg kg(-1) pyrene under four different treatments; inorganic fertilizer (if) alone, 15% (v ... | 2008 | 18632137 |
| papaya ringspot virus-p: characteristics, pathogenicity, sequence variability and control. | taxonomy: papaya ringspot virus (prsv) is an aphid-transmitted plant virus belonging to the genus potyvirus, family potyviridae, with a positive sense rna genome. prsv isolates belong to either one of two major strains, p or w. the p strains infect both papaya and cucurbits whereas the w strains infect only cucurbits. geographical distribution: prsv-p is found in all major papaya-growing areas. physical properties: virions are filamentous, non-enveloped and flexuous measuring 760-800 x 12 nm. vi ... | 2008 | 18705869 |
| an easy method using glutaraldehyde-introduced fluorescence for the microscopic analysis of plant biotrophic interactions. | the method introduced in this article makes use of the glutaraldehyde-induced auto-fluorescence of proteins after cross-linking with glutaraldehyde for the analysis of cellular and sub-cellular structures. because the interface of biotrophic interactions is rich in proteins, the method presented is particularly suitable for the analysis of such interactions; we have exemplified its usefulness by analyzing (1) the root feeding sites induced in roots from arabidopsis thaliana by the root-knot nema ... | 2008 | 18778431 |
| resistance and tolerance to herbivory changes with inbreeding and ontogeny in a wild gourd (cucurbitaceae). | herbivory is a ubiquitous component of terrestrial communities that reduces plant growth and reproduction. consequently, a goal of evolutionary ecology is to identify the causes and consequences of variation in herbivory within plant populations. this three-year study examined the effects of inbreeding on the resistance of wild gourd plants (cucurbita pepo subsp. texana) to herbivory by cucumber beetles and the impact of the timing of herbivory on reproduction. we grew families of inbred and out ... | 2008 | 21632318 |
| nanoparticles as smart treatment-delivery systems in plants: assessment of different techniques of microscopy for their visualization in plant tissues. | the great potential of using nanodevices as delivery systems to specific targets in living organisms was first explored for medical uses. in plants, the same principles can be applied for a broad range of uses, in particular to tackle infections. nanoparticles tagged to agrochemicals or other substances could reduce the damage to other plant tissues and the amount of chemicals released into the environment. to explore the benefits of applying nanotechnology to agriculture, the first stage is to ... | 2008 | 17998213 |
| characterization of esterases from cucurbita pepo cv. "eskandrani". | two of the six esterases identified in cucurbita pepo cv. "eskandrani" were purified to homogeneity using two chromatography steps: anion exchange and gel filtration. the molecular weights of c. pepo esterases eic and eii were 50,000 +/- 1500 and 68,000 +/- 1900 da from gel filtration and 47,000 and 66,000 da from sds/page, respectively, suggesting a monomeric structure for both enzymes. esterases eic and eii had k(m) values of 1.22 and 1.56 mm and ph optima at 9.0 and 8.0, respectively. the sub ... | 2008 | 17321740 |
| monitoring diabrotica v. virgifera (col.: chrysomelidae) in southeastern slovenia: increasing population trend and host spectrum expansion. | ever since the western corn rootworm (wcr) (diabrotica virgifera virgifera), an alien invasive species from north america, has been introduced into europe on at least 3 separate occasions, it spread within 15 years over the entire area of south-eastern and central europe (except denmark). until quite recently, zea mays l. was the only known host plant whereas in north america wcr also attacks members of the plant family cucurbitaceae. in august of 2006, we were able to validate these findings al ... | 2008 | 19226789 |
| differential uptake for dioxin-like compounds by zucchini subspecies. | the zucchini (cucurbita pepo) cultivars 'patty green', 'black beauty', and 'gold rush' were cultivated on weathered dioxin-contaminated soil in pots, and concentrations of the 29 dioxin-like compounds that were assigned who-tefs, three non-toxic polychlorinated dibenzo-p-dioxins/dibenzofurans (pcdd/fs), and two non-dioxin-like polychlorinated biphenyls (pcbs) were analyzed. toxic equivalent (teq) values accumulated in 'black beauty' and 'gold rush' were about 180 times higher than those in 'patt ... | 2008 | 18835616 |
| senescence progression in a single darkened cotyledon depends on the light status of the other cotyledon in cucurbita pepo (zucchini) seedlings: potential involvement of cytokinins and cytokinin oxidase/dehydrogenase activity. | darkness mediates different senescence-related responses depending on the targeting of dark treatment (whole plants or individual leaves) and on the organs that perceive the signal (leaves or cotyledons). as no data are available on the potential role of darkness to promote senescence when applied to individual cotyledons, we have investigated how darkness affects the progression of senescence in either a single or both individually darkened cotyledons of young 10-day-old cucurbita pepo (zucchin ... | 2008 | 18823328 |
| the effects of repeated planting, planting density, and specific transfer pathways on pcb uptake by cucurbita pepo grown in field conditions. | an in situ field investigation into the potential of pcb phytoextraction by cucurbita pepo ssp. pepo (pumpkin) plants was continued for a second year at a field site known to be contaminated with a mixture of aroclors 1254 and 1260 (average soil [pcb]=21 microg/g). plant stem and leaf pcb concentrations in this second field season (11 and 8.9 microg/g, respectively) were observed to increase significantly from the stem and leaf pcb concentrations reported in the previous year (5.7 and 3.9 microg ... | 2008 | 18786697 |
| differential effects of methyl jasmonate on growth and division of etiolated zucchini cotyledons. | the jasmonates are well studied in the context of plant defence but increasingly are also recognised as playing roles in development. in many systems, jasmonates antagonise the effects of cytokinins. the aim of the present work was to elucidate interactions between methyl jasmonate and cytokinin (benzyladenine) in regulating growth of zucchini (cucurbita pepo l., cv. cocozelle, var. tripolis) cotyledons, taking advantage of the ability to simultaneously quantify cell enlargement and division fro ... | 2008 | 18557907 |
| response of citrus peelminer marmara gulosa guillén and davis (lepidoptera: gracillariidae) stages to various insecticides. | egg and larval stages of citrus peelminer marmara gulosa guillén and davis infesting zucchini squash cucurbita pepo l. var 'revenue' were exposed in the laboratory to a wide range of insecticide classes used in california citrus (organophosphate, carbamate, pyrethroid, neonicotinoid, insect growth regulator, fermentation products and sulfur) to determine peelminer response with and without a non-ionic adjuvant or oil. | 2008 | 18506674 |
| ascorbic acid conjugates isolated from the phloem of cucurbitaceae. | analysis of phloem exudates from the fruit of cucurbitaceae revealed the presence of several compounds with uv-visible absorption spectra identical to that of l-ascorbic acid. in cucurbita pepo l. (zucchini), the compounds could be isolated from phloem exudates collected from aerial parts of the plant but were not detected in whole tissue homogenates. the compounds isolated from the phloem exudates of c. pepo fruit were eluted from strong anion exchange resin in the same fraction as l-ascorbic a ... | 2008 | 18472116 |
| diabrotica virgifera virgifera leconte: inconspicuous leaf beetle--formidable challenges to agriculture. | in the universe of entomology with its close to one million described and an estimated ten million undescribed species, diabrotica virgifera virgifera leconte (coleoptera: chrysometidae), the western corn rootworm (wcr), is one of the economically most important pests. it causes annual treatment costs and damages of a billion dollars in the us (metcalf 1986). similar costs are predicted for europe which the beetle invaded 15 years ago. due to lack of natural enemies it is now expanding its terri ... | 2007 | 18399421 |
| impact of daily consumption of iron fortified ready-to-eat cereal and pumpkin seed kernels (cucurbita pepo) on serum iron in adult women. | iron deficiency, anemia, is the most prevalent nutritional problem in the world today. the objective of this study was to consider the effectiveness of consumption of iron fortified ready-to-eat cereal and pumpkin seed kernels as two sources of dietary iron on status of iron nutrition and response of hematological characteristics of women at reproductive ages. eight healthy female, single or non pregnant subjects, aged 20-37 y consumed 30 g of iron fortified ready-to-eat cereal (providing 7.1 mg ... | 2007 | 18198398 |
| in vitro antioxidant properties of cucurbita pepo l. male and female flowers extracts. | total antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (dpph.), hydroxyl (ho.), scavenging activities, and total phenolic values were determined in extracts of cucurbita pepo l. female and male flowers. powdered c. pepo l. samples were extracted in aqueous ethyl acetate (ea: w1, 17:3), ethanol (e), and water (w) by agitating in magnetic stirrer for 80 degrees c, 15 min and also by in aqueous ethyl acetate (ea: w2, 17:3) at 25 degrees c, 15 min. dpph., ho. scavenging capacities and total phe ... | 2007 | 17308962 |
| in situ phytoextraction of polychlorinated biphenyl - (pcb)contaminated soil. | a pilot-scale field trial of phytoextraction of pcbs provides insight into the practical application of this technology, using the plant species cucurbita pepo ssp pepo cv. howden (pumpkin), carex normalis (sedge), and festuca arundinacea (tall fescue). this in situ trial took place at a historically contaminated field site, in soil contaminated with a mean concentration of 46 microg/g (range of 0.6 - 200 microg/g) total pcbs (aroclor 1254/1260). shoot bioaccumulation factors (where baf(shoot)=[ ... | 2007 | 17258285 |
| comparing contributions of soil versus root colonization to variations in stomatal behavior and soil drying in mycorrhizal sorghum bicolor and cucurbita pepo. | in prior studies we learned that colonization of soil can be as important as colonization of roots in determining mycorrhizal influence on the water relations of host plants. here we use a path analysis modeling approach to test (a) whether quantity of hyphae in soil contributes to variations in stomatal behavior and soil drying, and (b) whether soil colonization or root colonization has a stronger influence on these stomatal and soil drying responses. experiments were performed on sorghum bicol ... | 2007 | 17189660 |
| reactions, characterization and uptake of ammoxidized kraft lignin labeled with 15n. | ammoxidation of kraft lignin was carried out in a parr reactor using (15)nh(3) as the main nitrogen source. reaction parameters were set up until a total nitrogen content of approximately 13 wt.% in lignin was achieved, in accordance with conditions of previous studies. analytical tools such as ftir, py-gc/ms, and solid state nmr were used in this research. the nature of nitrogen bondings is discussed. the incorporation of the (15)n from ammoxidized lignin was followed in pumpkins (zucchini cucu ... | 2007 | 17161600 |
| isotopic enrichment in a phloem-feeding insect: influences of nutrient and water availability. | the isotopic enrichment between an animal and its diet can vary among and within living systems, but the sources of variation are not yet fully understood. some studies have found that diet quality or an animal's nutritional status can influence the degree of trophic enrichment, while others have dismissed nutrition as a contributing factor. we evaluated the effects of nutrient and water availability on carbon and nitrogen isotopic enrichment in a specialized plant-herbivore system. aphids are l ... | 2007 | 17124569 |
| the effect of stem pruning and nitrogen levels of on some physico-chemical characteristics of pumpkin seed (cucurbita pepo l.). | to investigate the effects of stem pruning (no heading, head pruning of stem after formation of 10 and 14 nodes) and nitrogen levels (0, 50, 100 and 200 kg ha(-1)) on physical and chemical characteristic of pumpkin seed a factorial experiment based on randomized complete block design with three replication was carried out in gorgan at 2003 and repeated in 2004 years. results showed that the stem pruning has significant effect on traits such as seed oil, linoleic acid and oleic acid content. nitr ... | 2007 | 19093491 |
| reversible change of extracellular ph at the generation of mechano-induced electrical reaction in a stem of cucurbita pepo. | as was shown recently the generation of cooling-induced action potential accompanies the reversible change of extracellular ph.1 the ph changes are linked to the ca(2+)-induced transient inhibition of the plasma membrane h(+)-atpase. generation of mechano-induced local bioelectrical reaction (lber) also accompanies the reversible change of extracellular ph. shifts of ph medium during generation of mechano-induced electrical impulse are less than during generation of cooling-induced bioelectrical ... | 2007 | 19704676 |
| surfactants differentially impact p,p'-dde accumulation by plant and earthworm species. | the effect of four surfactants (triton x-100, tween-80, rhamnolipids, cyclodextrin) at 100-1000 mg/l on p,p'-dde phytoextraction by cucurbita pepo (zucchini) under field conditions and p,p'-dde bioaccumulation by earthworm species (eisenia fetida, lumbricus terrestris) under laboratory conditions was investigated. abiotically, surfactants (except cyclodextrin) increased contaminant desorption from soil by 4-fold, with higher concentrations generally promoting greater release. cyclodextrin had no ... | 2007 | 17533859 |
| ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro. | ultrasonic treatment (0.5-2 min) stimulated multiple shoot regeneration to high levels in vitro from recalcitrant cotyledon explants of commercial squash (cucurbita pepo l.) cultivars ma'yan and bareqet, on murashige and skoog [physiol plant 15:473-497, 1962] (regeneration) medium augmented with 4.4 microm benzyladenine. at this stage, unsonicated control explants regenerated only a few very small shoots or bud-like structures. ultrasound also stimulated massive explant growth. ultrasound treatm ... | 2007 | 17021848 |
| methyl jasmonate is a more effective senescence-promoting factor in cucurbita pepo (zucchini) cotyledons when compared with darkness at the early stage of senescence. | the effects of short-term darkening and methyl jasmonate (meja) on cotyledon senescence were studied 24h after transfer of intact 7-day-old cucurbita pepo (zucchini) seedlings to darkness or spraying with 100 microm meja. the jasmonate inhibitory effect on chlorophyll content and chloroplast transcriptional activity was stronger compared with darkness. further, meja reduced the photosynthetic rate whereas darkness did not affect photosynthesis. neither stress factor affected the photochemical qu ... | 2007 | 16987568 |
| nectar resorption and translocation in cucurbita pepo l. and platanthera chlorantha custer (rchb.). | nectar resorption and sugar translocation were studied in cucurbita pepo (cucurbitaceae) and platanthera chlorantha (orchidaceae) by micro-autoradiography. in both species, nectar was resorbed in pollinated and unpollinated flowers and ovules developing into seeds were found to be the main sugar sink. in c. pepo, the mobility of resorbed sugars in pollinated female flowers was higher than in unpollinated ones; male flowers showed lower mobility of resorbed sugar. in p. chlorantha, radioactivity ... | 2007 | 16883483 |
| [lipoxidase in cucurbita pepo roots]. | 2007 | 13628795 | |
| ascorbic acid oxidase is dynamically regulated by light and oxygen. a tool for oxygen management in plants? | ascorbic acid oxidase (aao) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. in spite of extensive molecular characterization the physiological role of aao is still uncertain. abundant mrna, protein and activity of aao were observed in illuminated leaves of cucurbita pepo. aao activity was found to be proportional to light intensity. the light effect was rapidly reversed in dark and activity remained low throughout the dark period. ... | 2007 | 16343690 |
| artificial elevation of glutathione affects symptom development in zymv-infected cucurbita pepo l. plants. | styrian oil pumpkin seedlings (cucurbita pepo l. subsp. pepo var. styriaca greb: .) were treated for 48 h with 1 mm otc (l-2-oxothiazolidine-4-carboxylic acid) in order to artificially increase cellular glutathione content. they were inoculated with zucchini yellow mosaic virus (zymv) 10 days later. the effects of otc treatment and zymv infection on glutathione levels were examined at the subcellular level by immunogold labeling of glutathione using a transmission electron microscope (tem). thes ... | 2007 | 17143780 |
| virus-induced changes in the subcellular distribution of glutathione precursors in cucurbita pepo (l.). | changes in glutathione contents occur in plants during environmental stress situations, such as pathogen attack, as the formation of reactive oxygen species leads to the activation of the antioxidative defence system. as glutathione is synthesized out of its constituents cysteine, glycine, and glutamate the availability of these components will limit glutathione synthesis in plants especially during stress situations and therefore the ability of the plant to fight oxidative stress. to gain a dee ... | 2007 | 17143806 |
| membrane associated qualitative differences in cell ultrastructure of chemically and high pressure cryofixed plant cells. | membrane contrast can sometimes be poor in biological samples after high pressure freezing (hpf) and freeze substitution (fs). the addition of water to the fs-medium has been shown to improve membrane contrast in animal tissue and yeast. in the present study we tested the effects of 1% and 5% water added to the fs-medium (2% osmium with 0.2% uranyl acetate in anhydrous acetone) on the quality and visibility of membranes in high pressure frozen leaf samples of cucurbita pepo l. plants and compare ... | 2007 | 17270463 |
| ethnomedicines used in trinidad and tobago for reproductive problems. | throughout history women have tried to control or enhance their fertility using herbal remedies, with various levels of societal support. caribbean folk medicine has been influenced by european folk medicine, either through the early spanish and french settlers or through the continuous immigration of spanish-speaking peoples from venezuela. some folk uses are ancient and were documented by galen and pliny the elder. | 2007 | 17362507 |
| chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated. | chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. to study the role and regulation of chlorophyllase (chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (citrus sinensis) chlase in two heterologous plant systems: (1) squash (cucurbita pepo) plants using a viral vector expression system; ... | 2007 | 17369368 |
| physiological and metabolic changes of cucurbita pepo leaves in response to zucchini yellow mosaic virus (zymv) infection and salicylic acid treatments. | the changes of some physiological and biochemical parameters in pumpkin (cucurbita pepo cv eskandarani) leaves associated with zucchini yellow mosaic virus (zymv) infection and the effect of exogenous application of salicylic acid (sa) were studied in this paper. in comparison to the untreated leaves, zymv infected leaves showed many symptoms, including severe mosaic, size reduction, stunting and deformation. results from analysis of physiological parameters indicated that viral infection and sa ... | 2007 | 17466528 |
| ethnoveterinary medicines used to treat endoparasites and stomach problems in pigs and pets in british columbia, canada. | this paper documents the medicinal plants used to treat endoparasites and stomach problems in dogs, cats and pigs in british columbia, canada. ethnoveterinary data was collected over a 6-month period in 2003. the majority of the information on pets came from 2 naturopaths, 10 herbalists, 5 dog trainers, breeders and pet shop owners, 9 holistic veterinarians and 6 of 27 organic farmers. two pig farmers joined the study in the final stages. the following plants were used as anthelmintics: artemisi ... | 2007 | 17628343 |
| content of nutrient and antinutrient in edible flowers of wild plants in mexico. | nutrient and antinutritional/toxic factors present in some edible flowers consumed in mexico were determined. the edible flowers were: agave salmiana, aloe vera, arbutus xalapensis, cucurbita pepo (cultivated), erythrina americana, erythrina caribaea, euphorbia radians benth and yucca filifera. the nutrient content in the flowers studied is similar to that of the edible leaves and flowers studied mainly in africa. the moisture content of the flowers varied from 860 to 932 g kg(-1). crude protein ... | 2007 | 17768684 |
| zucchini yellow mosaic virus: insect transmission and pathogenicity -the tails of two proteins. | summary taxonomy: zucchini yellow mosaic virus (zymv) is a member of genus potyvirus, family potyviridae. zymv is a positive-strand rna virus. physical properties: virions are flexuous filaments of 680-730 nm in length and 11-13 nm in diameter, composed of about 2000 subunits of a single 31-kda protein (calculated). the genome rna size is 9.6 kb covalently linked to a viral-encoded protein (the vpg) at the 5' end, and with a 3' poly a tail. the 5' end of the sequence is au-rich (69%). viral prot ... | 2007 | 20507486 |
| identification and characterization of a novel whitefly-transmitted member of the family potyviridae isolated from cucurbits in florida. | abstract a novel whitefly-transmitted member of the family potyviridae was isolated from a squash plant (cucurbita pepo) with vein yellowing symptoms in florida. the virus, for which the name squash vein yellowing virus (sqvyv) is proposed, has flexuous rod-shaped particles of approximately 840 nm in length. the experimental host range was limited to species in the family cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the fami ... | 2007 | 18944369 |
| qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo. | squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ... | 2007 | 17444652 |
| differences in p,p'-dde bioaccumulation from compost and soil by the plants cucurbita pepo and cucurbita maxima and the earthworms eisenia fetida and lumbricus terrestris. | two plant species, cucurbita pepo and cucurbita maxima, and two earthworm species, eisenia fetida and lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-dde contamination. pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in c. pepo. bioaccumulation by e. fetida was approximately 6- and 3-fold higher than that by l. terrestris in the soil and compost, respectively. for all species, p,p'-dde uptake was significantly greater fr ... | 2007 | 17241722 |
| uptake and translocation of p,p'-dichlorodiphenyldichloroethylene supplied in hydroponics solution to cucurbita. | field studies show shoots of zucchini (cucurbita pepo l.) accumulate various hydrophobic contaminants from soil, although many other plants do not, including cucumber (cucumis sativus l.). to investigate the mechanism for this uptake, we presented p,p'-dichlorodiphenyldichloroethylene (dde) to these two species in hydroponics solution. a mixture of dde bound to tenax beads stirred with a solution of water passing through a reservoir provided a flowing solution containing dde at approximately 2 m ... | 2007 | 18020671 |
| phytoextraction of excess soil phosphorus. | in the search for a suitable plant to be used in p phytoremediation, several species belonging to legume, vegetable and herb crops were grown in p-enriched soils, and screened for p accumulation potentials. a large variation in p concentrations of different plant species was observed. some vegetable species such as cucumber (cucumis sativus) and yellow squash (cucurbita pepo var. melopepo) were identified as potential p accumulators with >1% (dry weight) p in their shoots. these plants also disp ... | 2007 | 16904249 |
| assessment of attractiveness of plants as roosting sites for the melon fly, bactrocera cucurbitae, and oriental fruit fly, bactrocera dorsalis. | the use of toxic protein bait sprays to suppress melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), populations typically involves application to vegetation bordering agricultural host areas where the adults seek shelter ("roost"). although bait spray applications for suppression of oriental fruit fly, bactrocera dorsalis (hendel), populations have traditionally been applied to the host crop, rather than to crop borders, roosting by oriental fruit flies in borders of some crop ... | 2007 | 20334596 |
| [relationships between host preference of liriomyza huidobrensis (blanchard) and nutrient and chlorophyll contents in host foliage]. | the laboratory study with no free choice means showed that the host preference of liriomyza huidobrensis (blanchard) adult differed significantly with test plants. phaseolus vulgaris, chrysanthemum coronarium and cucurbita pepo were the most preferred, while lycopersicum esculenturn and brassica oleracea were the least. correlative analysis indicated that the host preference of l. huidobrensis adult had a significant positive correlation with the content of soluble sugar, but less correlation wi ... | 2007 | 17552217 |
| protein inhibitors of trypsin from the seeds of cucurbitaceae plants. | seven trypsin inhibitors were isolated from the seeds of cucurbitaceae plants: two from cucumber (cucumis sativus) and red bryony (bryonia diotica) and one from figleaf gourd (cucurbita ficifolia), spaghetti squash (cucurbita pepo var. (vegetable spaghetti) and water melon (citrullus vulgaris). the inhibitors were purified by fractionation with ammonium sulphate, followed by ion-exchange chromatography and affinity chromatography using immobilized trypsin or anhydro-trypsin. the homogeneous inhi ... | 2007 | 3450102 |
| molecular cloning and characterization of patellin1, a novel sec14-related protein, from zucchini (cucurbita pepo). | a full-length patellin1 (patl1) cdna was cloned and characterized from zucchini (cucurbita pepo). patl1, originally discovered in the higher plant arabidopsis thaliana, is a plant sec14-related protein that localizes to the cell plate during the late stages of cytokinesis. patl1 is related in sequence to other eukaryotic proteins involved in membrane trafficking and is thought to participate in vesicle trafficking events associated with cell plate maturation. the zucchini patl1 (cppatl1) cdna pr ... | 2006 | 16542754 |
| influence of citric acid amendments on the availability of weathered pcbs to plant and earthworm species. | a series of small and large pot trials were conducted to assess the phytoextraction potential of several plant species for weathered polychlorinated biphenyls (pcbs) in soil (105 microg/g arochlor 1268). in addition, the effect of citric acid on pcb bioavailability to both plants and earthworms was assessed. under small pot conditions (one plant, 400 g soil), three cucurbits (cucurbita pepo ssp pepo [zucchini] and ssp ovifera [nonzucchini summer squash], cucumis sativus, cucumber) accumulated up ... | 2006 | 16615308 |
| accumulation of weathered polycyclic aromatic hydrocarbons (pahs) by plant and earthworm species. | experiments were conducted to assess the bioavailability of polyclycic aromatic hydrocarbons (pahs) in soil from a manufactured gas plant site. three plant species were cultivated for four consecutive growing cycles (28 days each) in soil contaminated with 36.3 microg/g total pah. during the first growth period, cucurbita pepo ssp. pepo (zucchini) tissues contained significantly greater quantities of pahs than did cucumis sativus (cucumber) and cucurbita pepo ssp. ovifera (squash). during the fi ... | 2006 | 16337258 |
| inbreeding effects on blossom volatiles in cucurbita pepo subsp. texana (cucurbitaceae). | self-pollination by plants gives rise to inbreeding depression. there is increasing recognition that plant inbreeding can have significant implications for interactions between plants and other organisms, including insects and pathogens. many of these interactions are mediated by plant-derived volatiles, but the effects of inbreeding on volatile production have not previously been investigated. we examined variation in flower volatile production by the wild gourd cucurbita pepo subsp. texana as ... | 2006 | 21642122 |
| detection by rapd-pcr of polymorphism in populations of bemisia tabaci (genn.) collected on four host plants from iran. | the sweetpotato whitefly, bernisia tabaci (genn.) (hom: aleyrodidae) is a major pest of field crops, vegetables and ornamentals in iran. in this study, the infested leaves of cucumber (cucurnis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from iran, and were transferred to the laboratory. the newly emerged males and females of each population were released separately into a large cage set ... | 2006 | 17385530 |
| extracts from pygeum africanum and other ethnobotanical species with antiandrogenic activity. | extracts from pygeum africanum, serenoa repens and cucurbita pepo are used in the treatment of benign prostatic hyperplasia (bph) and prostate cancer (pca). the activity of the androgen receptor (ar) is known to control growth of the prostate. here, we examined extracts of these plants for their antiandrogenic activity using an ar responsive reporter gene assay for drug discovery. a selective dichloromethane extract from the stem barks of pygeum africanum revealed the highest antiandrogenic effe ... | 2006 | 16783690 |
| in vitro antioxidative activity of pumpkin seed (cucurbita pepo) protein isolate and its in vivo effect on alanine transaminase and aspartate transaminase in acetaminophen-induced liver injury in low protein fed rats. | the antioxidative effects of pumpkin seed protein isolate (cucurbita pepo) were investigated in vitro. the isolate exhibited about 80% radical scavenging activity, chelating activity of approximately 64% on fe2+ ions and an inhibition of approximately 10% of xanthine oxidase. subsequently the effects of the isolate on the plasma activity levels of alanine transaminase and aspartate transaminase against acetaminophen induced acute liver injury in low-protein fed male sprague-dawley rats were asce ... | 2006 | 16807884 |
| inhibition of testosterone-induced hyperplasia of the prostate of sprague-dawley rats by pumpkin seed oil. | the oil from the pumpkin (cucurbita pepo) seed is claimed to be useful in the management of benign prostatic hyperplasia. this investigation seeks to examine the effect of pumpkin seed oil on testosterone-induced hyperplasia of the prostate of rats. hyperplasia was induced by subcutaneous administration of testosterone (0.3 mg/100 g of body weight) for 20 days. simultaneous oral administration of either pumpkin seed oil (2.0 and 4.0 mg/100 g of body weight) or corn oil (vehicle) was also given f ... | 2006 | 16822218 |