Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| thermal and chemical denaturation of bacillus circulans xylanase: a biophysical chemistry laboratory module. | a number of institutions have been, or are in the process of, modifying their biochemistry major to include some emphasis on the quantitative physical chemistry of biomolecules. sometimes this is done as a replacement for part for the entire physical chemistry requirement, while at other institutions this is incorporated as a component into the traditional two-semester biochemistry series. the latter is the model used for biochemistry and molecular biology majors at the university of richmond, w ... | 2008 | 21591232 |
| decolorization of azo dyes and simulated dye bath wastewater using acclimatized microbial consortium--biostimulation and halo tolerance. | anaerobic acclimatization of activated sludge from a textile effluent treatment plant to high concentration of rb5 could effectively decolorize rb5 dye solution. the strains viz. pseudomonas aeruginosa and bacillus circulans and other unidentified laboratory isolates (nad1 and nad6) were predominantly present in the microbial consortium. the conditions for efficient decolorization, biostimulation to increase effectiveness of microbial consortium, its tolerance to high salt concentration and non- ... | 2008 | 17566726 |
| use of metabolic and molecular methods for the identification of a bacillus strain isolated from paper affected by foxing. | foxing of paper is a deterioration phenomenon occurring in the form of brown-yellowish spots, the abiotic and/or biotic causes of which are not yet completely understood. nevertheless, microbiological infection has been recognized that may contribute to paper damage and therefore it becomes important to know the taxonomic position and the degradative activity of the potential infectious biological agents which mostly are fungi, but also bacteria and yeasts. a cellulolytic bacterial strain isolat ... | 2008 | 17686620 |
| bacillus circulans wz-12 - a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium. | a novel dichloromethane (dcm)-degrading bacterial strain named wz-12 (genbank accession no. ef100968) was isolated and identified as bacillus circulans based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16s ribosomal deoxyribonucleic acid. dcm dehalogenase from b. circulans wz-12 was purified to 8.27-fold with a yield of 34.83%. the electrophoretically homogeneous-purified enzyme exhibited a specific activity of 118.82 u/mg. s ... | 2007 | 17687552 |
| terpenoids and flavonoids from laggera pterodonta. | to study the chemical constituents of aerial parts of laggera pterodonta (dc.) benth., the air-dried aerial parts of this plant were powered and extracted with boiling water and purified by silica gel column chromatography and recrystallized. eleven compounds were obtained from l. pterodonta. they were identified as to be 6-o-beta-d-glucopyranosyl-carvotanacetone (1), pterodontic acid (2), 1beta-hydroxy pterondontic acid (3), pterodontoside a (4), pterodondiol (5), pterodontriol b (6), 5-hydroxy ... | 2007 | 17703774 |
| competitive inhibition bacteria of bovine origin against salmonella serovars. | studies were conducted to isolate bacteria inhibitory to salmonella enterica serovar typhimurium definitive type (dt) 104 in vitro from cattle not carrying salmonella and to determine the inhibitory activity of the isolated bacteria through competitive growth in cattle feces artificially contaminated with salmonella typhimurium dt104 and s. enterica serovar newport. fecal samples (108) were obtained from dairy and beef cows. s. enterica serovars were isolated from 9.25% of the samples and includ ... | 2007 | 17803135 |
| a secondary xylan-binding site enhances the catalytic activity of a single-domain family 11 glycoside hydrolase. | bacillus circulans xylanase (bcx) is a single-domain family 11 glycoside hydrolase. using nmr-monitored titrations, we discovered that an inactive variant of this enzyme, e78q-bcx, bound xylooligosaccharides not only within its pronounced active site (as) cleft, but also at a distal surface region. chemical shift perturbation mapping and affinity electrophoresis, combined with mutational studies, identified the xylan-specific secondary binding site (sbs) as a shallow groove lined by asn, ser, an ... | 2007 | 17822716 |
| production of cyclodextrin glycosyltransferase by alkaliphilic bacillus circulans in submerged and solid-state cultivation. | cyclodextrin glycosyltransferase (cgtase; e.c. 2.4.1.19) is an industrially important enzyme, which is used to produce cyclodextrins (cds). in this research, we report the use of experimental factorial design to find the best conditions of ph and temperature for cgtase production by bacillus circulans var. alkalophilus. the optimized calculated values for the tested variables were, respectively, ph 9.7 and temperature 36 degrees c, with a cgtase activity of 615 u ml(-1). the cgtase production wa ... | 2007 | 17574546 |
| effect on epidermal cell of soybean protein-degraded products and structural determination of the root hair promoting peptide. | peptide(s) produced from degraded soybean protein by an alkaline protease from bacillus circulans ha12 (degraded soybean-meal products; dsp) increased the number of both the root hair cells (trichoblasts) and hairless cells (atrichoblasts) of brassica rapa by about 4.4 times and 1.9 times, respectively. to identify the root hair-promoting peptide(s) in dsp, the origin protein of the root hair-promoting peptide(s) was identified as kunitz trypsin inhibitor (kti). the root hair-promoting peptide i ... | 2007 | 17671783 |
| cyclodextrin production by cyclodextrin glycosyltransferase from bacillus circulans df 9r. | cyclodextrins (cd) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. in this work, the conditions used to produce cd with cyclodextrin glycosyltransferase from bacillus circulans df 9r were optimized using experimental designs. the developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the cd production, using the same cassava starch as enzyme ad ... | 2007 | 17174549 |
| elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. | the proteins neo-11 and neo-18 encoded in the neomycin gene cluster (neo) of streptomyces fradiae ncimb 8233 have been characterized as glucosaminyl-6'-oxidase and 6'-oxoglucosaminyl:l-glutamate aminotransferase, respectively. the joint activity of neo-11 and neo-18 is responsible for the conversion of paromamine to neamine in the biosynthetic pathway of neomycin through a mechanism of fad-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination. neo-18 is also show ... | 2007 | 17206729 |
| characterization of the enzyme btrd from bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin. | 2007 | 17226887 | |
| mutanase from a paenibacillus isolate: nucleotide sequence of the gene and properties of recombinant enzymes. | a mutanase (alpha-1,3-glucanase)-producing microorganism was isolated from a soil sample and was identified as a relative of paenibacillus sp. the mutanase was purified to homogeneity from culture, and its molecular mass was around 57 kda. the gene for the mutanase was cloned by pcr using primers based on the n-terminal amino acid sequence of the purified enzyme. the determined nucleotide sequence of the gene consisted of 3651-bp open reading frame that encoded a predicted 1217-amino acid polype ... | 2007 | 17270351 |
| accurate, conformation-dependent predictions of solvent effects on protein ionization constants. | predicting how aqueous solvent modulates the conformational transitions and influences the pka values that regulate the biological functions of biomolecules remains an unsolved challenge. to address this problem, we developed fdpb_mf, a rotamer repacking method that exhaustively samples side chain conformational space and rigorously calculates multibody protein-solvent interactions. fdpb_mf predicts the effects on pka values of various solvent exposures, large ionic strength variations, strong e ... | 2007 | 17360348 |
| efficient production of 2-deoxy-scyllo-inosose from d-glucose by metabolically engineered recombinant escherichia coli. | 2-deoxy-scyllo-inosose (doi) is a six-membered carbocycle formed from d-glucose-6-phosphate catalyzed by 2-deoxy-scyllo-inosose synthase (dois), a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics. doi is valuable as a starting material for the benzene-free synthesis of catechol and other benzenoids. we constructed a series of metabolically engineered escherichia coli strains by introducing a dois gene (btrc) from bacillus circulans and disrupting genes fo ... | 2007 | 17368605 |
| a novel cyclic isomaltooligosaccharide (cycloisomaltodecaose, ci-10) produced by bacillus circulans t-3040 displays remarkable inclusion ability compared with cyclodextrins. | cyclodextrans (cis) are cyclic isomaltooligosaccharides and only ci-7, ci-8, and ci-9 were known. ci-7, ci-8, and ci-9, consisting of seven, eight, and nine glucoses, respectively, bound by alpha-(1-->6) linkages, are known to be produced by t-3040 strain of bacillus circulans. however, we have found, using 13c nmr and mass spectrometry, that this strain also produces ci-10, ci-11 and ci-12. these large cis are very soluble in water and inhibit the glucan synthesis of glucansucrases to the same ... | 2007 | 17433485 |
| biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain. | butirosin, an aminoglycoside antibiotic produced by bacillus circulans, bears the unique (s)-4-amino-2-hydroxybutyrate (ahba) side chain, which protects the antibiotic from several common resistance mechanisms. the ahba side chain is advantageously incorporated into clinically valuable antibiotics such as amikacin and arbekacin by synthetic methods. therefore, it is of significant interest to explore the biosynthetic origins of this useful moiety. we report here that the ahba side chain of butir ... | 2007 | 17462573 |
| unique o-ribosylation in the biosynthesis of butirosin. | using a comparative genetics approach, one or more of the btra, btrl, btrp, and btrv proteins encoded in the butirosin biosynthetic gene cluster (btr) from bacillus circulans sank72073 were identified as being responsible for an o-ribosylation process leading to the formation of ribostamycin, a key intermediate in this, and related antibiotic biosynthetic pathways. functional analysis of the recombinantly expressed proteins revealed that both btrl and btrp were responsible for the ribosylation o ... | 2007 | 17482823 |
| biological pretreatment with two bacterial strains for enzymatic hydrolysis of office paper. | the cellulose-hydrolyzing strains, sphingomonas paucimobilis mk1 and bacillus circulans mk2, were separated from soil and were grown together in a single culture plate. growth b. circulans mk2 in liquid culture required symbiosis with s. paucimobilis mk1. biological pretreatment with the combined strain suspension after the liquid culture improved enzymatic hydrolysis of office paper from municipal wastes. sugar recovery by s. paucimobilis mk1 (51%) was 1.4 times higher than that of the untreate ... | 2007 | 17487532 |
| recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from families 10 and 11. | the contributions of enzyme-substrate hydrogen-binding interactions to catalysis by two different families of xylanases were evaluated through kinetic studies with two representative wild-type enzymes, cellulomonas fimi xylanase (cex) and bacillus circulans xylanase (bcx), on a series of monodeoxygenated and monodeoxyfluorinated p-nitrophenyl xylobioside substrates. effects of substitution in the distal (-2 subsite) sugar on kcat/km for cex were moderately large (up to 2.9 kcal mol-1), with no e ... | 2007 | 17503782 |
| role of glutamate 243 in the active site of 2-deoxy-scyllo-inosose synthase from bacillus circulans. | 2-deoxy-scyllo-inosose (doi) synthase is involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics and catalyzes the carbocyclic formation from d-glucose-6-phosphate (g-6-p) into doi. the reaction mechanism is proposed to be similar to that of dehydroquinate (dhq) synthase in the shikimate pathway, and includes oxidation of c-4, beta-elimination of phosphate, reduction of c-4, ring opening, and intramolecular aldol cyclization. to investigate the reaction mechanism ... | 2007 | 17035031 |
| mechanism of stabilization of bacillus circulans xylanase upon the introduction of disulfide bonds. | the introduction of disulfide bonds has been used as a strategy to enhance the stability of bacillus circulans xylanase. the transition temperature of the s100c/n148c (ds1), v98c/a152c (ds2), and a1gc/g187,c188 (cxl) in comparison to the wild type was increased by 5.0, 4.1 and 3.8 degrees c, respectively. interestingly, a combination of two disulfide bonds of ds1 and cxl (cds1, circular disulfide 1) led to a 12 degrees c increase in the transition temperature. importantly, an increase in the mel ... | 2007 | 17141401 |
| characterization and mechanistic study of a radical sam dehydrogenase in the biosynthesis of butirosin. | btrn encoded in the butirosin biosynthetic gene cluster possesses a cxxxcxxc motif conserved within the radical s-adenosyl methionine (sam) superfamily. its gene disruption in the butirosin producer bacillus circulans caused the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine (doia) and 2-deoxystreptamine (dos). further, in vitro assay of the overexpressed enzyme revealed that btrn catalyzed the oxidation of doia under the strictly anaerobic conditions along with consum ... | 2007 | 18001019 |
| characterization of nocardiopsis beta-1,3-glucanase with additional carbohydrate-binding domains. | beta-1,3-glucanase f (bglf) from alkaliphilic nocardiopsis sp. f96 is a single domain enzyme composed of only a catalytic domain. chimeric bglfs with some carbohydrate-binding domains were constructed and characterized. by connecting the c-terminal additional domain of beta-1,3-glucanase h from bacillus circulans iam1165 and the chitin-binding domain of chitinase j from alkaliphilic bacillus sp. j813, binding ability and hydrolyzing activity toward insoluble beta-1,3-glucans were both improved. | 2007 | 18029785 |
| antagonistic and antimicrobial activities of some bacterial isolates collected from soil samples. | thirty seven bacterial cultures isolated from soil samples obtained from different locations were tested for their antagonistic activity against some fungal pathogens, viz., sclerotium rolfsii, fusarium oxysporum and rhizoctonia solani, causal agents of collar rot of sunflower, wilts and root rots, respectively. among them, 5 bacterial strains, viz., a1 6 (bacillus sphaericus), k1 24 (pseudomonas fluorescens), m1 42 (bacillus circulans), m1 66 (bacillus brevis) and t1 22 (bacillus brevis) showed ... | 2007 | 23100644 |
| an alkaline protease from bacillus circulans bm15, newly isolated from a mangrove station: characterization and application in laundry detergent formulations. | an investigation on the properties of an alkaline protease secreted by bacillus circulans bm15 strain isolated from a mangrove sediment sample was carried out in order to characterize the enzyme and to test its potency as a detergent additive. the protease was purified to apparent homogeneity by ammonium sulphate precipitation and was a 30-kda protease as shown by sds-page and its proteolytic activity was detected by casein zymography. it had optimum activity at ph 7, was stable at alkaline ph r ... | 2007 | 23100681 |
| cloning, sequencing, and expression of the genes encoding an isocyclomaltooligosaccharide glucanotransferase and an alpha-amylase from a bacillus circulans strain. | the gene for a novel glucanotransferase, isocyclomaltooligosaccharide glucanotransferase (igty), involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}] from starch, was cloned from the genome of b. circulans am7. the igty gene, designated igty, consisted of 2,985 bp encoding a signal peptide of 35 amino acids and a mature protein of 960 amino ac ... | 2006 | 17090949 |
| crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. | we report here on crystallization and preliminary x-ray analysis of the catalytic domain of chitinase d from bacillus circulans wl-12. the native crystals of this domain were found to belong to the orthorhombic space group p2(1)2(1)2(1). to elucidate the structure of the catalytic domain by the multiple isomorphous replacement method, 30 kinds of derivatized crystals were prepared by soaking the native crystals into a mother liquor containing salts of heavy metal atoms. difference patterson maps ... | 2006 | 17100652 |
| production of lewis x tetrasaccharides by metabolically engineered escherichia coli. | two tetrasaccharides carrying the trisaccharidic lewis x motif on a glcnac or a gal residue were produced on the gram-scale by high-cell-density cultures of metabolically engineered escherichia coli strains that overexpressed the helicobacter pylori futa gene for alpha-3 fucosyltransferase and the neisseria meningitidis lgtb gene for beta-4 galactosyltransferase. the first compound galbeta-4(fucalpha-3)glcnacbeta-4glcnac was produced by glycosylation of chitinbiose, which was endogenously genera ... | 2006 | 16381046 |
| enrichment and isolation of a mixed bacterial culture for complete mineralization of endosulfan. | in the present study, we isolated three novel bacterial species, namely, staphylococcus sp., bacillus circulans-i, and bacillus circulans-ii, from contaminated soil collected from the premises of a pesticide manufacturing industry. batch experiments were conducted using both mixed and pure cultures to assess their potential for the degradation of aqueous endosulfan in aerobic and facultative anaerobic condition. the influence of supplementary carbon (dextrose) source on endosulfan degradation wa ... | 2006 | 16393897 |
| identification of the substrate interaction region of the chitin-binding domain of streptomyces griseus chitinase c. | chitinase c from streptomyces griseus hut6037 was discovered as the first bacterial chitinase in family 19 other than chitinases found in higher plants. chitinase c comprises two domains: a chitin-binding domain (chbd(chic)) for attachment to chitin and a chitin-catalytic domain for digesting chitin. the structure of chbd(chic) was determined by means of 13c-, 15n-, and 1h-resonance nuclear magnetic resonance (nmr) spectroscopy. the conformation of its backbone comprised two beta-sheets composed ... | 2006 | 16567413 |
| anti-apoptotic activity of laminarin polysaccharides and their enzymatically hydrolyzed oligosaccharides from laminaria japonica. | laminarin polysaccharides (lp1) were prepared from laminaria japonica, a marine brown alga with potential biological activities, by hot water extraction, ultrafiltration and gel chromatography; the molecular weights of the lp1s were between 5 and 10 kda. laminarin oligosaccharides (lo) derived by hydrolyzing lp1 with an endo-beta-(1-->3)-glucanase from bacillus circulans were mainly di- and penta-oligosaccharides. treatment of mouse thymocytes with lo or lp1 (1-4 mg ml(-1)) suppressed apoptotic ... | 2006 | 16614911 |
| enzymatic production of highly soluble myricitrin glycosides using beta-galactosidase. | myricitrin, a botanical flavonol glycoside, could be a useful ingredient of functional foods, cosmetics, and medicines because of its high anti-oxidative activity. however, due to its insolubility in water, it has a limited range of use. to improve this solubility, we glycosylated myricitrin by an enzymatic transglycosylate reaction. myricitrin was galactosylated by beta-galactosidase from bacillus circulans using lactose as a sugar donor. the reaction product was 480 times more soluble than myr ... | 2006 | 16636462 |
| bioremediation of endosulfan contaminated soil and water -- optimization of operating conditions in laboratory scale reactors. | a mixed bacterial culture consisted of staphylococcus sp., bacillus circulans-i and -ii has been enriched from contaminated soil collected from the vicinity of an endosulfan processing industry. the degradation of endosulfan by mixed bacterial culture was studied in aerobic and facultative anaerobic conditions via batch experiments with an initial endosulfan concentration of 50mg/l. after 3 weeks of incubation, mixed bacterial culture was able to degrade 71.58+/-0.2% and 75.88+/-0.2% of endosulf ... | 2006 | 16730891 |
| bacillus anthracis virulent plasmid px02 genes found in large plasmids of two other bacillus species. | in order to cause the disease anthrax, bacillus anthracis requires two plasmids, px01 and px02, which carry toxin and capsule genes, respectively, that are used as genetic targets in the laboratory detection of the bacterium. clinical, forensic, and environmental samples that test positive by pcr protocols established by the centers for disease control and prevention for b. anthracis are considered to be potentially b. anthracis until confirmed by culture and a secondary battery of tests. we rep ... | 2006 | 16825351 |
| cloning and expression of an alpha-1,3-glucanase gene from bacillus circulans ka-304: the enzyme participates in protoplast formation of schizophyllum commune. | a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune has an activity to form protoplasts from s. commune mycelia, and a combination of alpha-1,3-glucanase and chitinase i, which were isolated from the filtrate, brings about the protoplast-forming activity. the gene of alpha-1,3-glucanase was cloned from b. circulans ka-304. it consists of 3,879 nucleotides, which encodes 1,293 amino acids including a putative signal peptide (31 amino acid resi ... | 2006 | 16861810 |
| crystal structures of the plp- and pmp-bound forms of btrr, a dual functional aminotransferase involved in butirosin biosynthesis. | the aminotransferase (btrr), which is involved in the biosynthesis of butirosin, a 2-deoxystreptamine (2-dos)-containing aminoglycoside antibiotic produced by bacillus circulans, catalyses the pyridoxal phosphate (plp)-dependent transamination reaction both of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and of amino-dideoxy-scyllo-inosose to 2-dos. the high-resolution crystal structures of the plp- and pmp-bound forms of btrr aminotransferase from b. circulans were solved at resolutions o ... | 2006 | 16894611 |
| structure of full-length bacterial chitinase containing two fibronectin type iii domains revealed by small angle x-ray scattering. | chitinase a1 (chia1) from bacillus circulans wl-12 consists of an n-terminal catalytic domain, two fibronectin type iii domains (fniiids), and a c-terminal chitin-binding domain. the full-length structure of chia1 was studied by small angle x-ray scattering. the obtained low-resolution structure showed that chia1 is an elongated molecule with a length of approximately 145 a composed of a large globular head and a rod-like tail. combination with known high-resolution structures of individual chia ... | 2006 | 16899221 |
| identification of catalytic amino acids of cyclodextran glucanotransferase from bacillus circulans t-3040. | in glycoside hydrolase family 66 (see http://afmb.cnrs-mrs.fr/cazy/), cyclodextran glucanotransferase (citase) is the only transglycosylation enzyme, all the other family 66 enzymes being dextranases. to analyze the catalytic amino acids of citase, we modified citase chemically from the t-3040 strain of bacillus circulans with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (edc). edc inactivated the enzyme by following pseudo-first order kinetics. in addition, the substrates of an isomaltooligos ... | 2006 | 16926507 |
| a novel glucanotransferase from a bacillus circulans strain that produces a cyclomaltopentaose cyclized by an alpha-1,6-linkage. | a novel glucanotransferase, involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch, was purified to homogeneity from the culture supernatant of bacillus circulans am7. the pi was estimated to be 7.5. the molecular mass of the enzyme was estimated to be 184 kda by gel filtration and 106 kda by sds-page. these results suggest that the ... | 2006 | 16926508 |
| bacteriocins reduce campylobacter colonization and alter gut morphology in turkey poults. | campylobacter is a leading cause of food-borne illness in the united states. recent evidence has demonstrated that bacteriocins produced by bacillus circulans and paenibacillus polymyxa reduce cecal campylobacter colonization in broiler chickens infected with campylobacter jejuni. as campylobacter coli is the most prevalent campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against c. coli colonization and their inf ... | 2006 | 16977842 |
| purification, characterization, and gene analysis of cellulase (cel8a) from lysobacter sp. ib-9374. | an enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium lysobacter sp. ib-9374, a high lysyl endopeptidase-producing strain. the enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated cel8a. the purified cel8a had a molecular mass of 41 kda, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a ph optimum of 5.0 was found for the beta-1,4-glucanase activit ... | 2006 | 17031054 |
| production of isocyclomaltopentaose from starch using isocyclomaltooligosaccharide glucanotransferase. | production of a novel cyclomaltopentaose cyclized by an alpha-1,6-linkage, [icg5; cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}], from starch was performed using isocyclomaltooligosaccharide glucanotransferase (igtase) derived from bacillus circulans am7. the optimal conditions for icg5-production from partially hydrolyzed starch were as follows: substrate concentration, 1.0% (w/v); ph, 5.5; temperature, 45 degrees c; reactio ... | 2006 | 17151467 |
| characterisation of the microflora of attiéké, a fermented cassava product, during traditional small-scale preparation. | attiéké is a fermented cassava product consumed mainly in cote d'ivoire. the aim of this study was to characterise the attiéké fermentation by examining products from 15 small-scale production sites at various stages of its preparation. for the preparation of attiéké, fresh cassava is grated to a pulp and inoculated with 10% of a spontaneous traditional inoculum. the inocula contained aerobic mesophiles at mean numbers of 8.2 x 10(7) cfu/g and lactic and acetic acids at mean concentrations of 0. ... | 2006 | 16213052 |
| statistical optimization of thermo-tolerant xylanase activity from amazon isolated bacillus circulans on solid-state cultivation. | a 2(2) factorial design was performed to find the best conditions of ph and temperature for xylanolytic activity of bacillus circulans bl53 isolated from the amazon environment. solid-state cultivation was carried out on an inexpensive, abundant agro-industrial soybean residue. the central composite design (ccd) used for the analysis of treatment combinations showed that a second-order polynomial regression model was in good agreement with experimental results, with r(2) = 0.9369 (p < 0.05). the ... | 2006 | 16216495 |
| mapping of residues involved in the interaction between the bacillus subtilis xylanase a and proteinaceous wheat xylanase inhibitors. | the bacillus subtilis xylanase a was subjected to site-directed mutagenesis, aimed at changing the interaction with triticum aestivum xylanase inhibitor, the only wheat endogenous proteinaceous xylanase inhibitor interacting with this xylanase. the published structure of bacillus circulans xyna was used to target amino acids surrounding the active site cleft of b.subtilis xyna for mutation. twenty-two residues were mutated, resulting in 62 different variants. the catalytic activity of active mut ... | 2006 | 16517552 |
| engineering of cyclodextrin glucanotransferase on the cell surface of saccharomyces cerevisiae for improved cyclodextrin production. | the cyclodextrin glucanotransferase (cgtase) gene (cgt) from bacillus circulans 251 was cloned into plasmid pyd1, which allowed regulated expression, secretion, and detection. the expression of cgtase with a-agglutinin at the n-terminal end on the extracellular surface of saccharomyces cerevisiae was confirmed by immunofluorescence microscopy. this surface-anchored cgtase gave the yeast the ability to directly utilize starch as a sole carbon source and the ability to produce the anticipated prod ... | 2006 | 16517633 |
| beneficiation of iron ore slime using aspergillus niger and bacillus circulans. | studies were carried out on the removal of alumina from iron ore slime containing (%) fe(2)o(3) 75.7, al(2)o(3) 9.95, sio(2) 6.1, fe (total) 52.94 with the help of bacillus circulans and aspergillus niger. b. circulans and a. niger showed 39% and 38% alumina removal after six and 15 days of in situ leaching at 10% pulp density, respectively. culture filtrate leaching with a. niger removed 20% alumina at 2% pulp density with 13 day old culture filtrate. b. circulans was more efficient than a. nig ... | 2006 | 16531043 |
| effect of ph on the structure and stability of bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: thermodynamic and crystallographic studies. | ph is one of the key parameters that affect the stability and function of proteins. we have studied the effect of ph on the pyridoxal-5'-phosphate-dependent enzyme phosphoserine aminotransferase produced by the facultative alkaliphile bacillus circulans ssp. alkalophilus using thermodynamic and crystallographic analysis. enzymatic activity assay showed that the enzyme has maximum activity at ph 9.0 and relative activity less than 10% at ph 7.0. differential scanning calorimetry and circular dich ... | 2006 | 16532449 |
| an enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. | a bacterial strain am7, isolated from soil and identified as bacillus circulans, produced two kinds of novel cyclic oligosaccharides. the cyclic oligosaccharides were produced from amylose using a culture supernatant of the strain as the enzyme preparation. the major product was a cyclomaltopentaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. the other minor product was cyclomaltohexao ... | 2006 | 16545346 |
| bacillus circulans mh-k1 chitosanase: amino acid residues responsible for substrate binding. | to identify the amino acids responsible for the substrate binding of chitosanase from bacillus circulans mh-k1 (mh-k1 chitosanase), tyr148 and lys218 of the chitosanase were mutated to serine and proline, respectively, and the mutated chitosanases were characterized. the enzymatic activities of y148s and k218p were found to be 12.5% and 0.16% of the wild type, respectively. when the (glcn)3 binding ability to the chitosanase was evaluated by fluorescence spectroscopy and thermal unfolding experi ... | 2005 | 16272568 |
| efficient enzymatic synthesis of 4-methylumbelliferyl n-acetyllactosaminide and 4-methylumbelliferyl sialyl n-acetyllactosaminides employing beta-d-galactosidase and sialyltransferases. | 4-methylumbelliferyl n-acetyllactosaminide and 4-methylumbelliferyl sialyl n-acetyllactosaminides, which are used for the assay of sialytransferase, neuraminidase and fucosyltransferase, were synthesized, respectively, by the beta-d: -galactosidase from bacillus circulans and by a recombinant rat alpha2,3-(n)-sialyltransferase or rat liver alpha2,6-(n)-sialyltransferase with cmp-n-acetylneuraminic acid as donor. | 2005 | 16231217 |
| enzyme adaptation to alkaline ph: atomic resolution (1.08 a) structure of phosphoserine aminotransferase from bacillus alcalophilus. | the crystal structure of the vitamin b(6)-dependent enzyme phosphoserine aminotransferase from the obligatory alkaliphile bacillus alcalophilus has been determined at 1.08 a resolution. the model was refined to an r-factor of 11.7% (r(free) = 13.9%). the enzyme displays a narrow ph optimum of enzymatic activity at ph 9.0. the final structure was compared to the previously reported structure of the mesophilic phosphoserine aminotransferase from escherichia coli and to that of phosphoserine aminot ... | 2005 | 15608117 |
| isolation of bacillus circulans and paenibacillus polymyxa strains inhibitory to campylobacter jejuni and characterization of associated bacteriocins. | we evaluated anti-campylobacter activity among 365 bacillus and paenibacillus isolates from poultry production environments. one novel antagonistic bacillus circulans and three paenibacillus polymyxa strains were identified and further studied. cell-free ammonium sulfate precipitate (crude antimicrobial preparation) was obtained from each candidate culture. zones of campylobacter growth inhibition surrounding 10 microl of this crude antimicrobial preparation were quantified using a spot test. ca ... | 2005 | 15690798 |
| cloning and expression of a bacillus circulans ka-304 gene encoding chitinase i, which participates in protoplast formation of schizophyllum commune. | ka-prep, a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune, has an activity to form protoplasts from s. commune mycelia, and a combination of alpha-1,3-glucanase and chitinase i, isolated from ka-prep, brings about the protoplast-forming activity. the gene of chitinase i was cloned from b. circulans ka-304 into pgem-t easy vector. the gene consists of 1,239 nucleotides, which encodes 413 amino acids including a putative signal peptide (24 a ... | 2005 | 15784990 |
| chitin-binding domain based immobilization of d-hydantoinase. | chitin-binding domain (chbd) of chitinase a1 from bacillus circulans wl-12 comprises 45 amino acids and exhibits remarkably high specificity to chitin (hashimoto, m., ikegami, t., seino, s., ohuchi, n., fukada, h., sugiyama, j., shirakawa, m., watanabe, t., 2000. expression and characterization of the chitin-binding domain of chintinase a1 from b. circulans wl-12. j. bacteriol. 182, 3045-3054.). to investigate the feasibility of exploiting chbd as affinity tags to confine enzymes of interest on ... | 2005 | 15862357 |
| construction of chimeric cyclodextrin glucanotransferases from bacillus circulans a11 and paenibacillus macerans iam1243 and analysis of their product specificity. | three dna fragments of 7919 base pairs containing genes for beta-cyclodextrin glucanotransferase (cgtase, ec 2.4.1.19), an iron iii dicitrate transport protein-like protein and a partial coding sequence for putative ferrichrome abc transporter from bacillus circulans a11 were cloned and sequenced (genbank accession af302787). the dna sequence contained a cgtase open reading frame of 2139 base pairs, which encoded a polypeptide of 713 amino acid residues. the signal peptide constituted the n-term ... | 2005 | 16084934 |
| extended sequence and functional analysis of the butirosin biosynthetic gene cluster in bacillus circulans sank 72073. | butirosin produced by bacillus circulans is among the clinically important 2-deoxystreptamine containing aminoglycoside antibiotics and its unique structure is found in (s)-4-amino-2-hydroxyburyric acid substituted at c-1 of 2-deoxystreptamine. recently, the key part of the butirosin biosynthetic gene cluster has been identified from bacillus circulans sank 72073, however the whole gene for the biosynthesis awaited for identification. in the present study, we undertook extended analysis of the b ... | 2005 | 16156513 |
| rapid affinity purification processes for cyclodextrin glycosyltransferase from bacillus circulans. | two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (cgtase) from bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. the first method, optimised by a factorial design, gave an 80% cgtase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mm alpha-cyclodextrin. the purification factor was 17. aqueous two-phase partition yielded a 72% cgtase recovery in a two-s ... | 2005 | 16158259 |
| convenient enzymatic synthesis of a p-nitrophenyl oligosaccharide series of sialyl n-acetyllactosamine, sialyl le x and relevant compounds. | from the beta-d-gal-(1-->4)-beta-d-glcnac-oc6h4no2-p (1) prepared by the transglycosylation of beta-galactosidase from bacillus circulans, alpha-d-neu5ac-(2-->3)-beta-d-gal-(1-->4)-beta-d-glcnac-oc6h4no2-p (9) and alpha-d-neu5ac-(2-->6)-beta-d-gal-(1-->4)-beta-d-glcnac-oc6h4no2-p (10) were effectively synthesized with an equimolar ratio of cmp-neu5ac by recombinant rat alpha-(2-->3)-n-sialyltransferase and rat liver alpha-(2-->6)-n-sialyltransferase, respectively. the former enzyme also transfer ... | 2005 | 16169536 |
| alkaline protease production by an isolated bacillus circulans under solid-state fermentation using agroindustrial waste: process parameters optimization. | alkaline protease production using isolated bacillus circulans under solid-state fermentation environment was optimized by using taguchi orthogonal array (oa) experimental design (doe) methodology to understand the interaction of a large number of variables spanned by factors and their settings with a small number of experiments in order to economize the process optimization. the software-designed experiments with an oa worksheet of l-27 was selected to optimize fermentation (temperature, partic ... | 2005 | 16209541 |
| crystallization and x-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. | a recombinant 2-deoxy-scyllo-inosose synthase from bacillus circulans has been crystallized at 277 k using peg 4000 as precipitant. the diffraction pattern of the crystal extends to 2.30 a resolution at 100 k using synchrotron radiation at the photon factory. the crystals are monoclinic and belong to space group p2(1), with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 a, beta = 117.8 degrees. the presence of two molecules per asymmetric unit gives a crystal volume per protein weight (vm) of ... | 2005 | 16511136 |
| biological control of late leaf spot of peanut (arachis hypogaea) with chitinolytic bacteria. | abstract late leaf spot (lls), caused by phaeoisariopsis personata, is a foliar disease of groundnut or peanut (arachis hypogaea) with high economic and global importance. antifungal and chitinolytic bacillus circulans grs 243 and serratia marcescens gps 5, selected among a collection of 393 peanut-associated bacteria, were applied as a prophylactic foliar spray and tested for control of lls. chitin-supplemented application of b. circulans grs 243 and s. marcescens gps 5 resulted in improved bio ... | 2005 | 18943468 |
| reduction of starch granule size by expression of an engineered tandem starch-binding domain in potato plants. | granule size is an important parameter when using starch in industrial applications. an artificial tandem repeat of a family 20 starch-binding domain (sbd2) was engineered by two copies of the sbd derived from bacillus circulans cyclodextrin glycosyltransferase via the pro-thr-rich linker peptide from xyn10a from cellulomonas fimi. sbd2 and a single sbd were introduced into the amylose-free potato mutant, amf, using appropriate signal sequences. the accumulation of sbd2 into transgenic starch gr ... | 2004 | 17147616 |
| mechanism-based fluorescent labeling of beta-galactosidases. an efficient method in proteomics for glycoside hydrolases. | (4-n-5-dimethylaminonaphthalene-1-sulfonyl-2-difluoromethylphenyl)-beta-d-galactopyranoside was synthesized and successfully tested on beta-galactosidases from xanthomonas manihotis (wong-madden, s. t., and landry, d. glycobiology (1995) 5, 19-28 and taron, c. h., benner, j. s., hornstra, l. j., and guthrie, e. p. (1995) glycobiology 5, 603-610), escherichia coli (jacobson, r. h., zhang, x. j., dubose, r. f., and matthews, b. w. (1994) nature 369, 761-766), and bacillus circulans (fujimoto, h., ... | 2004 | 15308675 |
| gene cluster in micromonospora echinospora atcc15835 for the biosynthesis of the gentamicin c complex. | gentamicin is a 4,6-disubstituted aminocyclitol antibiotic complex synthesised by some members of the actinomycete genus micromonospora. in a search for the gentamicin biosynthetic gene cluster we identified, using a cosmid library approach, a region of the m. echinospora atcc15835 chromosome that encodes homologues of aminoglycoside biosynthesis genes including gntb-a close homologue of the 2-deoxy-scyllo-inosose synthase gene (btrc) from butirosin-producing bacillus circulans. insertional inac ... | 2004 | 15376556 |
| identification of an alkaline-tolerant cyclodextrin-metabolizing bacterium and characterization of its cyclodextrinase gene. | bacillus circulans a11, an alkaline-tolerant cyclodextrin-metabolizing bacterium isolated from south-east asian soil, was reidentified as paenibacillus sp. a11 based on 16s rrna gene sequence comparison, g + c content and cellular fatty acid composition. levels of similarity of the 16s rrna gene between strain a11 and the paenibacillus species were 90-99%, while similarity with bacillus circulans was only 86%. the major cellular fatty acid was anteiso-c15:0 which accounted for 59.3% of the total ... | 2004 | 15378529 |
| rhizosheath of sinai desert plants is a potential repository for associative diazotrophs. | among 42 plant species representing the flora of north sinai, two possessed sand grain sheath encasing the roots. they are panicum turgidum forssk. and stipagrostis scoparia (trin.and rupr.) dewinter. rhizosheaths, compared to surrounding free sand, accommodated higher population density of microorganisms including associative diazotrophs. isolates secured belonged to the species of bacillus circulans, paenib. macerans (bacillus macerans), enterobacter agglomerans, agrobacterium radiobacter and ... | 2004 | 15462528 |
| use of heterotrophic co2 assimilation as a measure of metabolic activity in planktonic and sessile bacteria. | we have examined whether assimilation of co2 can be used as a measure of metabolic activity in planktonic and sessile heterotrophic bacteria. co2 assimilation by environmental samples and pure cultures of heterotrophic bacteria was studied using 14co2 and 13co2 as tracers. heterotrophic growth on complex organic substrates resulted in assimilation of co2 into cell biomass by activated sludge, drinking water biofilm, and pure cultures of escherichia coli atcc 25922, es. coli atcc 13706, rhodococc ... | 2004 | 15488281 |
| bacillus circulans peritonitis in a patient treated with capd. | 2004 | 15490992 | |
| regioselectivity in beta-galactosidase-catalyzed transglycosylation for the enzymatic assembly of d-galactosyl-d-mannose. | the regioselectivity of beta-galactosidase derived from bacillus circulans atcc 31382 (beta-1,3-galactosidase) in transgalactosylation reactions using d-mannose as an acceptor was investigated. this d-mannose associated regioselectivity was found to be different from reactions using either glcnac or galnac as acceptors, not only for beta-1,3-galactosidase but also for beta-galactosidases of different origins. the relative hydrolysis rate of gal beta-pnp and d-galactosyl-d-mannoses, of various li ... | 2004 | 15502353 |
| cloning, expression, and characterization of a highly thermostable family 18 chitinase from rhodothermus marinus. | a family 18 chitinase gene chia from the thermophile rhodothermus marinus was cloned and expressed in escherichia coli. the gene consisted of an open reading frame of 1,131 nucleotides encoding a protein of 377 amino acids with a calculated molecular weight of 42,341 da. the deduced chia was a non-modular enzyme with one unique glycoside hydrolase family 18 catalytic domain. the catalytic domain exhibited 43% amino acid identity with bacillus circulans chitinase c. due to poor expression of chia ... | 2004 | 15583965 |
| over-expression of the gene (bglbc1) from bacillus circulans encoding an endo-beta-(1-->3),(1-->4)-glucanase useful for the preparation of oligosaccharides from barley beta-glucan. | an endo-beta-(1-->3),(1-->4)-glucanase gene (bglbc1) from bacillus circulans atcc21367 was modified by substituting its native promoter with a strong promoter, bj27x, to increase expression of the gene when cloned into b. subtilis rm125 and b. megaterium atcc14945. a 771-bp endo-beta-(1-->3),(1-->4)-glucanase open reading frame was inserted into a new shuttle plasmid, pblc771, by ligating the orf and pbe1, the latter of which contained the strong promoter, bj27x. b. subtilis , transformed with t ... | 2004 | 15604830 |
| improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge. | cyclodextrin glycosyltransferase (cgtase) catalyzes the formation of cyclodextrins from starch. among the cgtases with known three-dimensional structure, thermoanaerobacterium thermosulfurigenes cgtase has the highest thermostability. by replacing amino acid residues in the b-domain of bacillus circulans cgtase with those from t. thermosulfurigenes cgtase, we identified a b. circulans cgtase mutant (with n188d and k192r mutations), with a strongly increased activity half-life at 60 degrees c. as ... | 2004 | 14705029 |
| a chitinase with high activity toward partially n-acetylated chitosan from a new, moderately thermophilic, chitin-degrading bacterium, ralstonia sp. a-471. | a moderately thermophilic bacterium, strain a-471, capable of degrading chitin was isolated from a composting system of chitin-containing waste. analysis of the 16s rdna sequence revealed that the bacterium belongs to the genus ralstonia. a thermostable chitinase a ( ra-chia) was purified from culture fluid of the bacterium grown in colloidal chitin medium. purification of the enzyme was achieved mainly by exploiting its binding to the colloidal chitin. the molecular mass of the enzyme was estim ... | 2004 | 12802528 |
| optimization of cyclodextrin production from sago starch. | cyclodextrin (cd) is synthesized by bacterial cyclodextrin glycosyltransferase (cgtase) and is widely used in food, pharmaceutical, cosmetic, and agricultural industries. in this study, bacillus circulans cgtase was partially purified by ammonium sulfate precipitation at 50-70% saturation. the optimum ph and temperature for cd production from sago starch were found to be in the ranges of 4.5-5.0 and 55-60 degrees c, respectively. beta-cd was the predominant product, constituting 65% of all cd pr ... | 2004 | 14643985 |
| antiamylase-pullulanase enzyme monoclonals which specifically inhibit amylase or pullulanase activity. | monoclonal antibodies against amylase-pullulanase enzyme from bacillus circulans f-2 have been produced to locate and characterize the catalytic sites of the enzyme. the antibodies have been examined for inhibition of both enzyme activities of amylase and pullulanase and then classified into four types: type i which inhibited amylase activity, type ii which inhibited pullulanase activity, type iii which inhibited both enzyme activities, and type iv which had no effect on either enzyme activity. ... | 2004 | 14984202 |
| bacillus galactosidilyticus sp. nov., an alkali-tolerant beta-galactosidase producer. | a novel bacillus isolate from raw milk and four strains from diverse origins that were identified previously as bacillus lentus, bacillus firmus and bacillus circulans showed a high degree of similarity in amplified rdna restriction analysis, sds-page and routine phenotypic tests, whilst 16s rdna sequence comparisons and dna relatedness data showed that this taxon was different from related bacillus species. on the basis of these data, bacillus galactosidilyticus sp. nov. is proposed, with the t ... | 2004 | 15023985 |
| formation of lacnac mimetics employing novel donor substrates for enzymatic beta 1-->4 galactosylation. | in examining c-6 modified 4-nitrophenyl beta-d-galactopyranosides as donor structures the beta-galactosidase (bacillus circulans) revealed an unexpectedly broad substrate specificity which allowed successful syntheses of various disaccharide components. | 2004 | 15034616 |
| tight attachment of chitin-binding-domain-tagged proteins to surfaces coated with acetylated chitosan. | several excellent procedures for trapping tagged proteins have been devised, but many of these are expensive, cannot be used outside a limited ph range, fail to work in the presence of chaotropic agents, or are difficult to use. the chitin binding domain (cbd) of bacillus circulans chitinase, which binds to chitin matrices prepared from inexpensive reagents isolated from crab shells, is an alternative tag that can be used under a variety of ph and denaturing conditions. kits based on the interac ... | 2004 | 15051546 |
| expression of chitinase-encoding genes in bacillus thuringiensis and toxicity of engineered b. thuringiensis subsp. aizawai toward lymantria dispar larvae. | chitinase genes from aeromonas hydrophila and bacillus circulans no.4.1 were cloned into the plasmid phy300plk and designated as phya2 and phyb43, respectively. both plasmids were introduced into various strains of b. thuringiensis by electroporation. plasmid phyb43 was generally structurally stable, but showed lower segregrational stability than phya2 in b. thuringiensis subsp. aizawai when grown under nonselective conditions. the production of chitinase from b. thuringiensis subsp. aizawai har ... | 2004 | 15057461 |
| mutation of active site residues in the chitin-binding domain chbdchia1 from chitinase a1 of bacillus circulans alters substrate specificity: use of a green fluorescent protein binding assay. | a fluorescent binding assay was developed to investigate the effects of mutagenesis on the binding affinity and substrate specificity of the chitin-binding domain of chitinase a1 from bacillus circulans wl-12. the chitin-binding domain was genetically fused to the n-terminus of a green fluorescent protein, and the polyhistidine-tagged hybrid protein was expressed in escherichia coli. residues likely to be involved in the binding site were mutated and their contributions to binding and substrate ... | 2004 | 15158679 |
| inhibition of the growth of ascosphaera apis by bacillus and paenibacillus strains isolated from honey. | the fungus ascosphaera apis, the causative agent of chalkbrood disease in honeybee larvae, occurs throughout the world and is found in many beekeeping areas of argentina. the potential as biocontrol agents of 249 aerobic spore-forming bacterial antagonists isolated from honey samples was evaluated. each isolate was screened against a. apis by a central disk test assay. ten bacterial strains that showed the best antagonistic effect to a. apis were selected for further study and identified as baci ... | 2004 | 15174751 |
| enzymatic synthesis of a beta-d-galactopyranosyl cyclic tetrasaccharide by beta-galactosidases. | the galactosyl transfer reaction to cyclo-[-->6)-alpha-d-glcp-(1-->3)-alpha-d-glcp-(1-->6)-alpha-d-glcp-(1-->3)-alpha-d-glcp-(1-->] (cts) was examined using lactose as a donor and beta-galactosidases from aspergillus oryzae and bacillus circulans. the a. oryzae beta-galactosidase produced three galactosyl derivatives of cts. the main galactosyl derivative produced by the a. oryzae enzyme was identified as 6-o-beta-d-galactopyranosyl-cts, cyclo-[-->6)-alpha-d-glcp-(1-->3)-[beta-d-galp-(1-->6)]-al ... | 2004 | 15183734 |
| a chitinase indispensable for formation of protoplast of schizophyllum commune in basidiomycete-lytic enzyme preparation produced by bacillus circulans ka-304. | ka-prep, a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation of schizophyllum commune, has an activity to form protoplasts from s. commune mycelia. alpha-1,3-glucanase, which was isolated from an ammonium sulfate fraction of 0-30% saturation of ka-prep, gave the protoplast-forming activity to an ammonium sulfate fraction of 30-50% saturation of ka-prep, which contained chitinase(s) and beta-glucanase(s) but was inactive in the protoplast formation. chitinase(s) and b ... | 2004 | 15215595 |
| production of autoproteolytically subunit-assembled 7-beta-(4-carboxybutanamido)cephalosporanic acid (gl-7aca) acylase from pseudomonas sp. c427 using a chitin-binding domain. | 7-beta-(4-carboxybutanamido)cephalosporanic acid (gl-7aca) acylase from pseudomonas sp. c427 is known as a proteolytically processed bacterial enzyme. gl-7aca acylase from pseudomonas sp. c427 (c427) consists of alpha- and beta-subunits that are processed from a precursor peptide by removing the spacer peptide. a chitin-binding domain (cbd) of chitinase a1 derived from bacillus circulans was genetically fused into four different positions of the c427-encoding gene. in the four enzymes thereby pr ... | 2004 | 15221226 |
| evaluation of fly ash as a carrier for diazotrophs and phosphobacteria. | fly ash and its different combinations with soil (w/w) were tested to explore its possible use as a potential carrier for diazotrophs and phosphobacteria. azotobacter chroococcum, azospirillum brasilense and bacillus circulans showed their maximum viability in fly ash alone whereas pseudomonas striata proliferated most in soil:fly ash (1:1) combination. | 2004 | 15246443 |
| enhancement of the antifungal activity of bacillus subtilis f29-3 by the chitinase encoded by bacillus circulans chia gene. | bacillus subtilis f29-3 is an antagonistic bacterium against a wide range of fungal species. in order to determine the effect of chitinase on the antifungal activity of b. subtilis f29-3, a 2.4-kb dna fragment containing the chia gene of bacillus circulans wl-12 was ligated into a shuttle vector phy300plk and transformed into b. subtilis f29-3. a bioassay conducted on the culture supernatant showed that, in comparison to the b. subtilis control strain, b. subtilis f29-3 expressing the chia gene ... | 2004 | 15284891 |
| two-step purification of bacillus circulans chitinase a1 expressed in escherichia coli periplasm. | a protein purification procedure was developed to efficiently and effectively purify the target enzyme, chitinase a1 of bacillus circulans wl-12, from escherichia coli dh5alpha carrying the chia gene with its natural promoter in the plasmid pntu110. chitinase a1 was purified to apparent homogeneity from e. coli periplasm with a final recovery of 90.6%. two main steps were included in this protein purification procedure, ammonium sulfate precipitation (40% saturation) and anion-exchange chromatog ... | 2004 | 15294277 |
| enzymatic synthesis of n-acetyllactosamine in aqueous-organic reaction media. | beta-galactosidase from bacillus circulans was used for the biocatalytic transfer of d-galactose (d-gal) from o-nitrophenyl-beta-d-galactoside (onpg) to the o-4 position of 2-acetamido-2-deoxy-d-glucopyranose (d-glcnac) forming the disaccharide n-acetyllactosamine (lacnac, beta-d-gal-(1 --> 4)-d-glcnac). in order to investigate the potential of this biocatalytic synthesis, first the optimal reactant ratio in an aqueous buffer system was determined. on the basis of these standard conditions we th ... | 2003 | 14703960 |
| overproduction and secretion of bacillus circulans endo-beta-1,3-1,4-glucanase gene (bglbc1) in b. subtilis and b. megaterium. | a gene coding for endo-beta-1,3-1,4-glucanase (lichenase) containing a recombinant plasmid, pll200k, was transferred from bacillus circulans into a new shuttle plasmid, plls920, by ligating linearized dnas of pll200k and pub110. b. subtilis rm125 and b. megaterium atcc14945 transformed with plls920 produced the endo-beta-1,3-1,4-glucanase. the enzyme was produced during active growth with maximum activity. the b. subtilis (plls920) enzyme was 83 times (8522 mu ml(-1)) more active than that of th ... | 2003 | 14514048 |
| occurrence of a specific protein in basidiomycete-lytic enzyme preparation produced by bacillus circulans ka-304 inductively with a cell-wall preparation of schizophyllum commune. | ka-prep, a culture filtrate of bacillus circulans ka-304 grown on a cell-wall preparation (cwp) of schizophyllum commune, has been reported to have an activity to form protoplasts from s. commune mycelia. the sds-polyacrylamide gel electrophoreses described here demonstrated that a specific proteinous component (molecular weight: 150,000) occurred in ka-prep. the protein (p150t) was also formed in culture filtrates with cwp of several basidiomycetes, which could release the protoplasts, suggesti ... | 2003 | 14519984 |
| growth dynamics of bacillus circulans colony. | we have investigated the growth dynamics of bacillus circulans colony exhibiting the knotted-branching pattern by swarming on a hard agar medium. the knotted-branching pattern consists of many circular clusters, so-called subcolonies, and their trajectories. we analysed the processes of a subcolony because they are presumably the key elements for the formation of knotted-branching pattern. it was found that a subcolony has three processes, i.e. "generation", "growth", and "migration" by microsco ... | 2003 | 14559062 |
| increased production of antioxidative sesaminol glucosides from sesame oil cake through fermentation by bacillus circulans strain yus-2. | bacillus circulans strain yus-2 was isolated as the strongest antioxidant-producer in fermentation of sesame oil cake (soc, defatted residue yielded from sesame seed oil production). two major strong antioxidants from fermented soc were purified and identified as known sesaminol triglucoside and sesaminol diglucoside, however, our results demonstrated that the fermentation process with b. circulans yus-2 was highly effective to gain the extraction efficiency of the sesaminol glucosides. | 2003 | 14586130 |
| real-time molecular beacon nasba reveals hblc expression from bacillus spp. in milk. | nucleic acid sequence-based amplification (nasba) was applied in combination with a fluorescein-conjugated molecular beacon specific for a sequence flanked by transcript-specific primers in order to monitor hblc enterotoxin gene expression in real-time from milk separately contaminated with bacillus amyloliquefaciens, bacillus cereus, and bacillus circulans. maximal enterotoxin expression was noted following 16, 15, and 16 h, respectively, when grown in artificially contaminated nonfat dried mil ... | 2003 | 14592426 |
| generation of an affinity column for antibody purification by intein-mediated protein ligation. | coupling an antigenic peptide to a solid support is a crucial step in the affinity purification of a peptide-specific antibody. conventional methods for generating reactive agarose, cellulose or other matrices for peptide conjugation are laborious and can result in a significant amount of chemical waste. in this report, we present a novel method for the facile production of a peptide affinity column by employing intein-mediated protein ligation (ipl) in conjunction with chitin affinity chromatog ... | 2003 | 14604539 |
| phylogenetic relationships between bacillus species and related genera inferred from comparison of 3' end 16s rdna and 5' end 16s-23s its nucleotide sequences. | the nucleotide sequences of the 3' end of the 16s rdna and the 16s-23s internal transcribed spacer (its) of 40 bacillaceae species were determined. these included 21 bacillus, 9 paenibacillus, 6 brevibacillus, 2 geobacillus, 1 marinibacillus and 1 virgibacillus species. comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16s rrna coding region and a conserved 70 bp sequence located at the 5' end of the 16s-23s its of the 40 sp ... | 2003 | 12807189 |
| conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: the role of alanine 230 in acceptor subsite +1. | cyclodextrin glycosyltransferase (cgtase) preferably catalyzes transglycosylation reactions, whereas many other alpha-amylase family enzymes are hydrolases. despite the availability of three-dimensional structures of several transglycosylases and hydrolases of this family, the factors that determine the hydrolysis and transglycosylation specificity are far from understood. to identify the amino acid residues that are critical for the transglycosylation reaction specificity, we carried out error- ... | 2003 | 12809508 |
| self-organized pattern formation of a bacteria colony modeled by a reaction diffusion system and nucleation theory. | self-organized pattern formation is observed in bacterial colony growth. the recently reported knotted-branching pattern of the bacillus circulans colony consists of the trajectories of aggregates which grow, move, and reproduce simultaneously. we modeled these processes by combining a reaction diffusion system of nutrient dynamics, nucleation theory for aggregate generation, and individual based dynamics of motion and growth of aggregates. the branching pattern produced by computer simulation s ... | 2003 | 12857171 |
| metal tolerance and biosorption capacity of bacillus circulans strain eb1. | a heavy-metal-resistant bacterium bacillus sp., strain eb1 was isolated from heavy-metal-contaminated soil in the southeast region of turkey. based on 16s ribosomal dna sequencing, the microorganism was closely related to bacillus circulans. minimal inhibitory concentrations of metals (mics) for the bacterium were determined. bacillus eb1 exhibited high mic values for metals and a large spectrum of antibiotic resistance. the order of toxicity of the metals to the bacterium was cd=co>cu>ni>zn>mn ... | 2003 | 12892847 |