Publications

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the antibacterial activity and modes of li-f type antimicrobial peptides against bacillus cereus in vitro.li-fs are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (gram-positive bacteria and fungi). in this study, li-f-type antimicrobial peptides (amp-jsa9) composing of li-f03a, li-f03b, li-f04a, li-f04b and li-f05b were isolated from paenibacillus polymyxa jsa-9. to better understand the antimicrobial mechanism of amp-jsa9, the potency and action(s) of amp-jsa9 against bacillus cereus were examined.201728650559
isolation, selection, and biological characterization research of highly effective electricigens from mfcs for phenol degradation.the microbial fuel cells (mfcs) are recognized to be highly effective for the biodegradation of phenol. for isolating the phenol-degrading bacteria, the sample containing 500 mg/l phenol was collected from the mfcs. the strain (wl027) was identified basing on the 16s rrna gene analysis and phylogenetic analysis as bacillus cereus. the effects of ph, temperature, concentrations of phenol, heavy metal ions, and salt on the growth of strain as well as the degradation of phenol have been carefully s ...201728646380
enhanced antibacterial activity of ent-labdane derivatives of salvic acid (7α-hydroxy-8(17)-ent-labden-15-oic acid): effect of lipophilicity and the hydrogen bonding role in bacterial membrane interaction.in the present study, the antibacterial activity of several ent-labdane derivatives of salvic acid (7α-hydroxy-8(17)-ent-labden-15-oic acid) was evaluated in vitro against the gram-negative bacterium escherichia coli and the gram-positive bacteria staphylococcus aureus and bacillus cereus. for all of the compounds, the antibacterial activity was expressed as the minimum inhibitory concentration (mic) in liquid media and minimum inhibitory amount (mia) in solid media. structure activity relations ...201728644410
do multi-year applications of bacillus thuringiensis subsp. israelensis for control of mosquito larvae affect the abundance of b. cereus group populations in riparian wetland soils?bacillus thuringiensis subsp. israelensis (bti) is a soil-borne bacterium affiliated to the bacillus cereus group (bcg) and has been used in biocontrol products against nematoceran larvae for several decades. however, knowledge is limited on whether long-term bti application can affect the structure of indigenous communities of bcg and the overall abundance of bti. using species- and group-specific quantitative pcr assays, we measured the bcg- and bti-abundances in riparian wetlands in the river ...201728600590
a purely green synthesis of silver nanoparticles using carica papaya, manihot esculenta, and morinda citrifolia: synthesis and antibacterial evaluations.green procedure for synthesizing silver nanoparticles (agnps) is currently considered due to its economy and toxic-free effects. several existing works on synthesizing agnps using leaves extract still involve the use of physical or mechanical treatment such as heating or stirring, which consume a lot of energy. to extend and explore the green extraction philosophy, we report here the synthesis and antibacterial evaluations of a purely green procedure to synthesize agnps using carica papaya, mani ...201728597212
risk factors of catheter-related bloodstream infection caused by bacillus cereus: case-control study in 8 teaching hospitals in japan.in this multicenter, matched case-control study, patients diagnosed with catheter-related bloodstream infection (crbsi) caused by bacillus cereus (n = 108) were matched to controls (n = 269). in the multivariable analysis, administration of an amino acid preparation and an indwelling peripheral catheter were significant variables for b cereus-related crbsi.201728596019
soil acidification aggravates the occurrence of bacterial wilt in south china.soil acidification is a major problem in modern agricultural systems and is an important factor affecting the soil microbial community and soil health. however, little is known about the effect of soil acidification on soil-borne plant diseases. we performed a 4-year investigation in south china to evaluate the correlation between soil acidification and the occurrence of bacterial wilt. the results showed that the average soil ph in fields infected by bacterial wilt disease was much lower than t ...201728487678
self assembled snowball-like hybrid nanostructures comprising viburnum opulus l. extract and metal ions for antimicrobial and catalytic applications.herein, we report the production of novel organic-inorganic nanobio-catalytic and antimicrobial agents called "nanosnowball" (nsbs) with a rational design and elucidate the increase in the catalytic and antimicrobial activities. the nsbs resemble to guelder rose were formed of the extract of viburnum opulus (vo) as the organic component and copper (ii) ions (cu(2+)) as the inorganic component. the effects of the vo extract and cu(2+) concentrations on the morphology of nsbs were systematically e ...201728465062
paneth-cell-disruption-induced necrotizing enterocolitis in mice requires live bacteria and occurs independently of tlr4 signaling.necrotizing enterocolitis (nec) remains a leading cause of morbidity and mortality in premature infants. both human surgical specimens and animal models suggest a potential involvement of paneth cells in nec pathogenesis. paneth cells play critical roles in epithelial homeostasis, innate immunity and host-microbial interactions. yet, the complex interplay between paneth cell disruption, epithelial barrier dysfunction and microbial-driven inflammation remains unclear in the immature intestine. in ...201728450472
simulating intestinal growth conditions enhances toxin production of enteropathogenic bacillus cereus.bacillus cereus is a ubiquitous bacterial pathogen increasingly reported to be the causative agent of foodborne infections and intoxications. since the enterotoxins linked to the diarrheal form of food poising are foremost produced in the human intestine, the toxic potential of enteropathogenic b. cereus strains is difficult to predict from studies carried out under routine cultivation procedures. in this study, toxigenic properties of a panel of strains (n = 19) of diverse origin were compared ...201728446903
interaction of nanostructured tio2 biointerfaces with stem cells and biofilm-forming bacteria.nanostructured tio2 nanotubes (nts) of diameters from 15 to 100nm were fabricated by an electrochemical anodization process. biofilm-positive strains of bacillus cereus and pseudomonas aeruginosa behaved similarly on all tio2 nts as well as on native titanium (ti) foil. the adhesion and growth of mesenchymal stem cells (msc), embryonic stem cells (esc), and pure cardiomyocytes derived from esc exhibited significant differences. msc as well as esc were, in contrast to cardiomyocytes, able to adhe ...201728532058
syntrophic association of termite gut bacterial symbionts with bifunctional characteristics of cellulose degrading and polyhydroxyalkanoate producing bacteria.the guild between higher termites and their partnership with the diverse community of bacteria and archaea in their gut is a marvel evolutionary achievement. sustained attempts were made worldwide with a quest for identifying viable important biological macromolecule polyhydroxyalkanoate (pha) accumulating bacteria. termite gut serve as a novel source for bacteria with dual properties like pha production as well as cellulose degradation. among 40 isolates cultivated, 32.5% turned positive for pc ...201728528947
revealing the ability of a novel polysaccharide bioflocculant in bioremediation of heavy metals sensed in a vibrio bioluminescence reporter assay.a bioflocculant-producing bacterial strain, designated msi021, was isolated from the marine sponge dendrilla nigra and demonstrated 94% flocculation activity in a kaolin clay suspension. msi021 was identified as bacillus cereus based on phylogenetic affiliation and biochemical characteristics. the purified extra-cellular bioflocculant was chemically elucidated as a polysaccharide molecule. the polysaccharide bioflocculant was stable under both acidic and alkaline conditions (ph 2.0-10.0) and tem ...201728527323
diversity and probiotic potential of lactic acid bacteria isolated from horreh, a traditional iranian fermented food.the aim of this study was to evaluate the probiotic potential of lactic acid bacteria (lab) strains isolated from horreh. some probiotic properties, e.g., resistance to acid, bile tolerance, antibacterial activity, and antibiotic susceptibility, were investigated. a total of 140 gram-positive and catalase-negative isolates from horreh were subjected to identification and grouping by cultural methods and the 16s rrna sequencing. the new isolates were identified to be lactobacillus (fermentum, pla ...201728527125
epidemiological study of common diseases and their risk factors in camels in south punjab, pakistan.bacteriological study of mastitis along with common blood protozoan diseases were studied in dromedary camels in cholistan, dera ismail khan and rahim yar khan districts in south punjab, pakistan. for this purpose 300 camels were sampled randomly at different common grazing and watering point. for study of blood parasites clinically suspected and apparently healthy camels, 150 each, were sampled. an overall prevalence of 15%and 5% was recorded for trypanosomiasis and anaplasmosis respectively. t ...201728428133
infection-related complications during treatment for childhood acute lymphoblastic leukemia.comprehensive studies on neutropenia and infection-related complications in patients with acute lymphoblastic leukemia (all) are lacking.201728426102
effective thermal inactivation of the spores of bacillus cereus biofilms using microwave.microwave sterilization was performed to inactivate the spores of biofilms of bacillus cereus involved in foodborne illness. the sterilization conditions, such as the amount of water and the operating temperature and treatment time, were optimized using statistical analysis based on 15 runs of experimental results designed by the box-behnken method. statistical analysis showed that the optimal conditions for the inactivation of b. cereus biofilms were 14 ml of water, 108°c of temperature, and 15 ...201728423891
predicting genome terminus sequences of bacillus cereus-group bacteriophage using next generation sequencing data.most tailed bacteriophages (phages) feature linear dsdna genomes. characterizing novel phages requires an understanding of complete genome sequences, including the definition of genome physical ends.201728472946
release study and inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules against foodborne bacteria.the antibacterial property of thyme essential oil due to different volatile compounds, has been well documented in the literature. to overcome the high volatility of essential oil components, encapsulation has emerged as a new alternative. in this work, chitosan and thyme essential oil-loaded chitosan nanoparticles (teo-csnps) and nanocapsules (teo-csncs) were prepared by nanoprecipitation and nanoencapsulation, respectively. the morphology, encapsulation efficiency, release kinetics, and inhibi ...201728526346
the putative drug efflux systems of the bacillus cereus group.the bacillus cereus group of bacteria includes seven closely related species, three of which, b. anthracis, b. cereus and b. thuringiensis, are pathogens of humans, animals and/or insects. preliminary investigations into the transport capabilities of different bacterial lineages suggested that genes encoding putative efflux systems were unusually abundant in the b. cereus group compared to other bacteria. to explore the drug efflux potential of the b. cereus group all putative efflux systems wer ...201728472044
non-contact electromagnetic induction heating for eradicating bacteria and yeasts on biomaterials and possible relevance to orthopaedic implant infections: in vitro findings.infection of implants is a major problem in elective and trauma surgery. heating is an effective way to reduce the bacterial load in food preparation, and studies on hyperthermia treatment for cancer have shown that it is possible to heat metal objects with pulsed electromagnetic fields selectively (pemf), also known as induction heating. we therefore set out to answer the following research question: is non-contact induction heating of metallic implants effective in reducing bacterial load in v ...201728522446
spotlight on bacillus cereus and its food poisoning toxins. 201728521335
standardization of biopolymer production from seaweed associative bacteria.polyhydroxyalkanoate (pha) is the biodegradable plastic, which is shown the similar properties to the synthetic plastic. production of pha is expensive when compared with petrochemical based plastics costs and also hazardous to ecosystem. in order to overcome these drawbacks, some cheaper commercially available carbon sources like rice bran, coconut cake, palm jaggary, etc. can be used. the present study aimed to identify the potential pha producing bacterial strains from different seaweeds. bas ...201728404223
cereulide production by bacillus weihenstephanensis strains during growth at different ph values and temperatures.besides bacillus cereus, some strains of the psychrotolerant, potentially foodborne pathogen bacillus weihenstephanensis can produce the emetic toxine (cereulide). this toxin is a heat- and acid-stable cyclic dodecadepsipeptide that causes food intoxication with vomiting. however, some severe clinical cases with lethal outcomes have been described. if cereulide can be produced during refrigerated storage, it will not be inactivated by reheating food, representing an important risk of food intoxi ...201728399995
analysis of kudoa septempunctata as a cause of foodborne illness and its associated differential diagnosis.recently kudoa septempuctata in olive flounders is suggested as a cause of food poisoning, however whether kudoa septempuctata can affect human gastrointestinal systems is controversial and its pathogenecity remains unclear. in view of the field epidemiology, food poisonings caused by kudoa septempuctata should be distinguished from those caused by staphylococcus aureus and bacillus cereus.201728395399
decontamination of multiple heavy metals-containing effluents through microbial biotechnology.to decontaminate heavy metal-containing waste water, a microbial biotechnology was developed by using the synergy between sulfate reducing bacteria (srb), bacillus cereus (b. cereus) and camellia oleifera cake (coc). in this process the coc degradation assisted by b.cereus, created an anoxic environment and provided energy and nutrition for srb. both of b. cereus and srb played significant roles through biosorption, bioaccumulation and biosurfactant production. meanwhile, a flotation technology ...201728521206
evaluation of two standard and two chromogenic selective media for optimal growth and enumeration of isolates of 16 unique bacillus species.the genus bacillus is a group of gram-positive endospore-forming bacteria that can cause food poisoning and diarrheal illness in humans. a wide range of food products have been linked to foodborne outbreaks associated with these opportunistic pathogens. the u.s. food and drug administration recommends (in their bacteriological analytical manual) the use of bacara or mannitol egg yolk polymyxin (myp) agar plates and the most-probable-number (mpn) method for enumeration and confirmation of bacillu ...201728467187
a novel technique for sterilization using a power self-regulated single-mode microwave cavity.in this paper, a novel technique to achieve precise temperatures in food sterilization has been proposed. an accurate temperature profile is needed in order to reach a commitment between the total removal of pathogens inside the product and the preservation of nutritional and organoleptic characteristics. the minimal variation of the target temperature in the sample by means of a monitoring and control software platform, allowing temperature stabilization over 100 °c, is the main goal of this wo ...201728590423
evaluation of cytotoxicity of hematite nanoparticles in bacteria and human cell lines.metal nanoparticles have a great impact and even change the composition of soil microbial communities. this poses the risk of their accumulation in the ecosystem, which may call on health hazard. statistical techniques such as plackett-burman design, response surface methodology were used for optimizing medium constituents for bacillus cereus svk1 and other critical variables responsible for the production of biomolecules and biosynthesis of hematite nanoparticles. the effect of hematite nanopar ...201728586726
antibacterial and antioxidative activity of o-amine functionalized chitosan.cinnamaldehyde was immobilized to o-amine functionalized chitosan via a coupling reaction. fourier transform infrared spectroscopy confirmed n-cinnamyl substitution. wetting analyses demonstrate more hydrophobicity in the n-cinnamyl substituted o-amine functionalized chitosan compared to chitosan or unsubstituted o-amine functionalized chitosan. thermal gravimetric analysis and differential scanning calorimetry demonstrates that the prepared n-cinnamyl substituted o-amine functionalized chitosan ...201728504167
effect of glucose and olive oil as potential carbon sources on production of phas copolymer and tercopolymer by bacillus cereus fa11.in this study, the influence of different physicochemical parameters on the yield of polyhydroxyalkanoates (phas) produced by bacillus cereus fa11 is investigated. the physicochemical factors include ph, temperature, time, inoculum size and its age, agitation speed and composition of the glucose rich peptone deficient (grpd) medium. during two-stage fermentation, b. cereus fa11 produced a significantly high (p < 0.05) yield (80.59% w/w) of phas copolymer using grpd medium containing glucose (15  ...201728501987
lateral flow assay-based bacterial detection using engineered cell wall binding domains of a phage endolysin.the development of a cost-effective and efficient bacterial detection assay is essential for diagnostic fields, particularly in resource-poor settings. although antibodies have been widely used for bacterial capture, the production of soluble antibodies is still expensive and time-consuming. here, we developed a nitrocellulose-based lateral flow assay using cell wall binding domains (cbds) from phage as a recognition element and colloidal gold nanoparticles as a colorimetric signal for the detec ...201728494369
cyclic diarylheptanoids from corylus avellana green leafy covers: determination of their absolute configurations and evaluation of their antioxidant and antimicrobial activities.the methanol extract of the leafy covers of corylus avellana, source of the italian pgi (protected geographical indication) product "nocciola di giffoni", afforded two new cyclic diarylheptanoids, giffonins t and u (2 and 3), along with two known cyclic diarylheptanoids, a quinic acid, flavonoid-, and citric acid derivatives. the structures of giffonins t and u were determined as highly hydroxylated cyclic diarylheptanoids by 1d and 2d nmr experiments. their relative configurations were assigned ...201728520428
comparative genomic analysis reveals genetic features related to the virulence of bacillus cereus forc_013.bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. in the present study, we sequenced the complete genome of b. cereus forc_013 isolated from fried eel in south korea. to extend our understanding of the genomic characteristics of forc_013, we conducted a comparative analysis with the published genomes of other b. cereus strains.201728515790
former food products safety: microbiological quality and computer vision evaluation of packaging remnants contamination.the use of alternative feed ingredients in farm animal's diets can be an interesting choice from several standpoints, including safety. in this respect, this study investigated the safety features of selected former food products (ffps) intended for animal nutrition produced in the framework of the izs plv 06/14 rc project by an ffp processing plant. six ffp samples, both mash and pelleted, were analysed for the enumeration of total viable count (tvc) (iso 4833), enterobacteriaceae (iso 21528-1) ...201728513297
bacteriophage exclusion (brex): a novel anti-phage mechanism in the arsenal of bacterial defense system.the arm race between bacteria and phages leads to the development of many phage resistance mechanisms. bacteria are perpetually changing their strategy to avert phage infection and killing. recently, a study published that shed light on a novel bacterial defense system called bacteriophage exclusion (brex). brex system is a six-gene cassette in bacillus cereus which provides complete phage resistance to a broad range of phages, including lytic and temperate ones. it is a novel defense strategy w ...201728444888
surveillance of bacillus cereus isolates in korea from 2012 to 2014.to investigate the prevalence and toxin production characteristics of non-emetic and emetic bacillus cereus strains isolated via the laboratory surveillance system in korea.201728443227
aptamer-based trapping: enrichment of bacillus cereus spores for real-time pcr detection.aptamer-based trapping techniques are in general suitable to replace common antibody-based enrichment approaches. a time-consuming isolation or clean-up is often necessary during sample preparation, e.g. for the detection of spores. for the development of bioanalytical routine approaches, aptamers with a high affinity to b. cereus spores were applied for the establishment and validation of an aptamer-based trapping technique in milk with fat contents between 0.3 and 3.5%. thereby, enrichment fac ...201728478557
the phosphotransferase system gene ptsi in bacillus cereus regulates expression of soda2 and contributes to colonization of wheat roots.plant growth-promoting rhizobacteria effectively enhance plant growth and root colonization by the bacteria is a prerequisite during the process. bacillus cereus 905, a rhizosphere bacterium originally isolated from wheat roots, colonizes the wheat rhizosphere with a large population size. we previously showed that a manganese-containing superoxide dismutase (mnsod2), encoded by the soda2 gene, plays an important role in colonization of the wheat rhizosphere by b. cereus 905. in this study, we i ...201728478075
green synthesis, characterisation and bioactivity of plant-mediated silver nanoparticles using decalepis hamiltonii root extract.consistent search of plants for green synthesis of silver nanoparticles (snps) is an important arena in nanomedicine. this study focuses on synthesis of snps using bioreduction of silver nitrate (agno3) by aqueous root extract of decalepis hamiltonii. the biosynthesis of snps was monitored by uv-vis analysis at absorbance maxima 432 nm. the fluorescence emission spectra of snps illustrated the broad emission peak 450-483 nm at different excitation wavelengths. the surface characteristics were st ...201728476981
indole derivatives produced by the metagenome genes of the escherichia coli-harboring marine sponge discodermia calyx.three indole derivatives, a novel benzoxazine-indole hybrid (1) and two known indole trimers (2, 3), were isolated from the metagenomic library of the marine sponge discodermia calyx based on functional screening. their structures were elucidated by extensive spectroscopic analysis and comparison of their nmr data to that of known compounds. the antibacterial assay indicated that only compound 2 displayed significant antibacterial activity against bacillus cereus, with approximately 20 mm diamet ...201728441364
seasonal outbreak of bacillus bacteremia associated with contaminated linen in hong kong.a high seasonal incidence of bacillus bacteremia was associated with the use of contaminated hospital linens.201728475782
genome-wide investigation of biofilm formation in bacillus cereus.bacillus cereus is a soil-dwelling gram-positive bacterium capable of forming structured multicellular communities, or biofilms. however, the regulatory pathways controlling biofilm formation are less well understood in b. cereus in this work, we developed a method to study b. cereus biofilms formed at the air-liquid interface. we applied two genome-wide approaches, random transposon insertion mutagenesis to identify genes that are potentially important for biofilm formation, and transcriptome a ...201728432092
the spo0a-sini-sinr regulatory circuit plays an essential role in biofilm formation, nematicidal activities, and plant protection in bacillus cereus ar156.the rhizosphere bacterium bacillus cereus ar156 is capable of forming biofilms, killing nematodes, and protecting plants. however, the underlying molecular mechanisms of these processes are not well understood. in this study, we found that the isogenic mutants δbcspo0a and δbcsini have significantly reduced colonization and nematicidal activity in vitro and biological control efficacy on the tomato plant under greenhouse conditions. we further investigated the role of the spo0a-sini-sinr regulat ...201728430084
effects of culture conditions on the size, morphology and wet density of spores of bacillus cereus 569 and bacillus megaterium qm b1551.the influence of variable culture conditions on the size and wet density of spores of bacillus cereus and bacillus megaterium were examined in this work. culture temperature and initial ph was shown to have a significant impact on the size of both species, with increasingly alkaline culture media and elevated culture temperatures resulting in spores that were, on average, up to 25% reduced in volume. increasing concentrations of inorganic salts in sporulation media exerted differing effects on e ...201728419596
virulence analysis of bacillus cereus isolated after death of preterm neonates, nice, france, 2013.after the deaths of 2 preterm neonates with bacillus cereus systemic infection in the same intensive care unit, we investigated the pathogenic potential of this bacterium. genetic and virulence analysis indicated the neonates were infected with 2 different strains with a virulence potential similar to environmental strains, indicating likely patient immune response failure.201728418291
crystal structure of the flagellar chaperone flis from bacillus cereus and an invariant proline critical for flis dimerization and flagellin recognition.flis is a cytoplasmic flagellar chaperone for the flagellin, which polymerizes into filaments outside of the flagellated bacteria. cytoplasmic interaction between flis and flagellin is critical to retain the flagellin protein in a monomeric form, which is transported from the cytoplasm through the flagellar export apparatus to the extracellular space for filament assembly. defects in the flis protein directly diminish bacterial motility, pathogenicity, and viability. although the overall structu ...201728414127
antibacterial and antibiotic potentiating activities of tropical marine sponge extracts.increasing prevalence of antibiotic resistance has led research to focus on discovering new antimicrobial agents derived from the marine biome. although ample studies have investigated sponges for their bioactive metabolites with promising prospects in drug discovery, the potentiating effects of sponge extracts on antibiotics still remains to be expounded. the present study aimed to investigate the antibacterial capacity of seven tropical sponges collected from mauritian waters and their modulat ...201728392375
genetic diversity and population structure of the bacillus cereus group bacteria from diverse marine environments.the phylogenetic diversity of marine bacteria belonged to the bacillus cereus group has not been well investigated. here, we present the genetic diversity and population structure of 71 bacteria from diverse marine environments, using a multilocus sequence typing (mlst) approach and the analyses of digital dna-dna hybridization (dddh) and average nucleotide identity (ani) based on some representative genomic sequences. the mlst analysis demonstrated that these isolates were highly diverse and a ...201728386130
production and characterization of curcumin microcrystals and evaluation of the antimicrobial and sensory aspects in minimally processed carrots.nontoxic conserving agents are in demand by the food industry due to consumers concern about synthetic conservatives, especially in minimally processed food. the antimicrobial activity of curcumin, a natural phenolic compound, has been extensively investigated but hydrophobicity is an issue when applying curcumin to foodstuff. the objective of this work was to evaluate curcumin microcrystals as an antimicrobial agent in minimally processed carrots. the antimicrobial activity of curcumin microcry ...201728406506
ribosomal background of the bacillus cereus group thermotypes.in this study we reconstructed the architecture of bacillus cereus sensu lato population based on ribosomal proteins, and identified a link between the ribosomal proteins' variants and thermal groups (thermotypes) of the bacilli. the in silico phyloproteomic analysis of 55 ribosomal proteins (34 large and 21 small subunit r-proteins) of 421 strains, representing 14 well-established or plausible b. cereus sensu lato species, revealed several ribosomal clusters (r-clusters), which in general were ...201728406161
biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications.in the present study, we report a green methodology for the synthesis of silver and gold nanoparticles, using the root extract of the herbal medicinal plant korean red ginseng. the silver and gold nanoparticles were synthesized within 1 h and 10 min respectively. the nanoparticles generated were not aggregated, and remained stable for a long time, which suggests the nature of nanoparticles. the phytochemicals and ginsenosides present in the root extract assist in reducing and stabilizing the syn ...201625706249
design, synthesis and biological evaluation of 4-benzoyl-1-dichlorobenzoylthiosemicarbazides as potent gram-positive antibacterial agents.twelve 4-benzoyl-1-dichlorobenzoylthiosemicarbazides have been tested as potential antibacterials. all the compounds had mics between 0.49 and 15.63 µg/ml toward micrococcus luteus, bacillus cereus, bacillus subtilis and staphylococcus epidermidis indicating, in most cases, equipotent or even more effective action than cefuroxime. in order to clarify if the observed antibacterial effects are universal, further research were undertaken to test inhibitory potency of two most potent compounds 3 and ...201625897586
chemical constituents and biological activities of albizia myriophylla wood.albizia myriophylla benth (leguminosae) is a medicinal plant widely used in thailand and other asian countries as a folk medicine remedy for many ailments.201625894212
investigation of an outbreak of vomiting in nurseries in south east england, may 2012.on 30 may 2012, surrey and sussex health protection unit was called by five nurseries reporting children and staff with sudden onset vomiting approximately an hour after finishing their lunch that day. over the following 24 h 50 further nurseries supplied by the same company reported cases of vomiting (182 children, 18 staff affected). epidemiological investigations were undertaken in order to identify the cause of the outbreak and prevent further cases. investigations demonstrated a nursery-lev ...201626165194
antibacterial effect of the red sea soft coral sarcophyton trocheliophorum.the marine soft corals sarcophyton trocheliophorum crude extracts possessed antimicrobial activity towards pathogenic bacterial strains, i.e. bacillus cereus, salmonella typhi, escherichia coli, staphylococcus aureus and pseudomonas aeruginosa. bioassay-guided fractionation indicated that the antimicrobial effect was due to the presence of terpenoid bioactive derivatives. further biological assays of the n-hexane fractions were carried out using turbidity assay, inhibition zone assay and minimum ...201626186031
a novel antimicrobial flavonoid from the stem bark of commiphora pedunculata (kotschy & peyr.) engl.a new flavonoid, 2-(3,5-dihydroxy-4-methoxy-phenyl)-3,5-dihydroxy-8,8-dimethyl-2,3-dihydro-8h-pyrano[3,2]chromen-4-one, together with previously reported epicatechin was isolated from the ethyl acetate soluble fraction of the methanol extract of the stem bark of commiphora pedunculata. the structures of these compounds were elucidated based on extensive analysis of their spectral data, including 1 and 2d nmr. the compounds were active against 9 out of 12 tested microorganisms including a resista ...201625978097
effects of climate change on the persistence and dispersal of foodborne bacterial pathogens in the outdoor environment: a review.according to the intergovernmental panel on climate change (ipcc), warming of the climate system is unequivocal. over the coming century, warming trends such as increased duration and frequency of heat waves and hot extremes are expected in some areas, as well as increased intensity of some storm systems. climate-induced trends will impact the persistence and dispersal of foodborne pathogens in myriad ways, especially for environmentally ubiquitous and/or zoonotic microorganisms. animal hosts of ...201625612827
evaluating the efficiency of humic acid to remove micro-organisms from denture base material.to evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials.201625523597
chemistry and biological activities of tanacetum chiliophyllum var. oligocephalum extracts.tanacetum chiliophyllum (fisch. & mey.) var. oligocephalum (d.c.) sosn. collected in turkey was subjected to phytochemical and biological evaluations in this study. pure compounds were obtained from ethyl acetate extracts of the stems of the plant material. structures of isolated compounds were determined using spectral methods. seven known flavones, i.e., 5-hydroxy-3',4',6,7-tetramethoxyflavone, eupatilin (6-hydroxyluteolin-6,3',4'-trimethylether), cirsimaritin (scuttellarin-6,7-dimethylether), ...201624645497
effect of different in vitro culture extracts of black pepper (piper nigrum l.) on toxic metabolites-producing strains.in the present study, the effect of different in vitro cultures (callus, in vitro shoots) and commercially available peppercorn extract was investigated for its activity against toxic metabolite-producing strains (escherichia coli, pseudomonas aeroginosa, salmonella typhi, bacillus subtilis, bacillus cereus, staphylococcus aureus, and candida albicans). these in vitro cultures were extracted with ethanol, hexane, and chloroform, and the antipathogenic activity was determined by well-diffusion me ...201624193053
molecular phylogeny of heterotrophic nitrifiers and aerobic denitrifiers and their potential role in ammonium removal.to investigate the physiology and taxonomic composition of the key players of nitrification and denitrification processes in paddy fields, culture dependent and independent studies have been carried out. a total of 28 bacterial strains have been screened in which six were capable of reducing nitrate and nitrite as well as having significant ammonium removal potential. 16s rrna-pcr-dgge-based molecular typing of enriched batch culture was done with time duration to explore and identify dominant a ...201627037833
antibacterial effects of alchornea cordifolia (schumach. and thonn.) müll. arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens.the leaves, stems and roots of alchornea cordifolia (schumach. and thonn.) müll. arg. are used as traditional medicine in many african countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds.201626724423
effects of potential probiotic bacillus cereus en25 on growth, immunity and disease resistance of juvenile sea cucumber apostichopus japonicus.this study was conducted to determine effects of potential probiotic bacillus cereus en25 (isolated from mud of sea cucumber culturing water bodies) on growth, immunity and disease resistance against vibrio splendidus infection in juvenile sea cucumbers apostichopus japonicus. animals were respectively fed diets with b. cereus en25 at 0 (control), 10(5), 10(7) and 10(9) cfu/g for 30 days. results showed that dietary b. cereus en25 had no significant effects on growth, total coelomocytes counts a ...201626723266
influence of season and type of restaurants on sashimi microbiota.in recent years, an increase in the consumption of japanese food in european countries has been verified, including in portugal. these specialities made with raw fish, typical japanese meals, have been prepared in typical and on non-typical restaurants, and represent a challenge to risk analysis on haccp plans. the aim of this study was to evaluate the influence of the type of restaurant, season and type of fish used on sashimi microbiota.201626911490
occurrence and antibacterial susceptibility pattern of bacterial pathogens isolated from diarrheal patients in pakistan.to determine the occurrence of bacterial pathogens responsible for diarrhea and to engender information regarding the effectiveness of commonly used antibiotic against diarrhea.201626905349
use of peripheral parenteral nutrition solutions as a risk factor for bacillus cereus peripheral venous catheter-associated bloodstream infection at a japanese tertiary care hospital: a case-control study.the risk factors are unclear for peripheral venous catheter-associated bloodstream infections (pvcbsis) caused by bacillus cereus. we aimed to examine for these risk factors in patients with b. cereus pvcbsi by conducting a 2-year case-control study in a large teaching hospital. we analyzed all adult cases of b. cereus pvcbsi (37 patients) and 180 controls who were randomly selected from among patients who had a pvc in place for at least 2 days. multivariate analysis using a conditional logistic ...201626902219
bacteria-based polythene degradation products: gc-ms analysis and toxicity testing.polythene degradation leads to the production of various by-products depending upon the type of degradation process. the polythene degradation products (pedp) in the culture supernatant of the two bacteria (lysinibacillus fusiformis strain vasb14/wl and bacillus cereus strain vasb1/ts) were analyzed with gc-ms technique. the major by-products in the pedp in the culture supernatant of l. fusiformis strain vasb14/wl (1,2,3,4 tetra methyl benzene) and b. cereus strain vasb1/ts (1,2,3 trimethyl benz ...201626888528
association of genotyping of bacillus cereus with clinical features of post-traumatic endophthalmitis.bacillus cereus is the second most frequent cause of post-traumatic bacterial endophthalmitis. although genotyping of b. cereus associated with gastrointestinal infections has been reported, little is known about the b. cereus clinical isolates associated with post-traumatic endophthalmitis. this is largely due to the limited number of clinical strains available isolated from infected tissues of patients with post-traumatic endophthalmitis. in this study, we report successful isolation of twenty ...201626886446
evaluation of bacterial contamination of dental unit waterlines and use of a newly designed measurement device to assess retraction of a dental chair unit.dental unit waterline (duwl) output water is delivered through instruments of a dental chair unit (dcu) to irrigate and cool teeth. however, these waterlines can be heavily contaminated with bacteria.201627000421
shelf life of donkey milk subjected to different treatment and storage conditions.the aim of this study was to investigate the effects of different treatment conditions on microbiological indicators of donkey milk hygiene and their evolution during shelf life at 4 and 12°c from 3 to 30d, simulating a farm-scale pasteurization and packing system. four treatment conditions were tested: no treatment (raw milk), pasteurization (65°c × 30 min), high-pressure processing (hpp), and pasteurization plus hpp. the microbiological quality of the raw donkey milk investigated was not optim ...201626995124
purification and characterization of plantaricin jla-9: a novel bacteriocin against bacillus spp. produced by lactobacillus plantarum jla-9 from suan-tsai, a traditional chinese fermented cabbage.bacteriocins are ribosomally synthesized peptides with antimicrobial activity produced by numerous bacteria. a novel bacteriocin-producing strain, lactobacillus plantarum jla-9, isolated from suan-tsai, a traditional chinese fermented cabbage, was screened and identified by its physiobiochemical characteristics and 16s rdna sequence analysis. a new bacteriocin, designated plantaricin jla-9, was purified using butanol extraction, gel filtration, and reverse-phase high-performance liquid chromatog ...201626985692
formation and biopharmaceutical characterization of electrospun pvp mats with propolis and silver nanoparticles for fast releasing wound dressing.antibacterial, antiviral, antifungal, antioxidant, anti-inflammatory, and anticancer activities of propolis and its ability to stimulate the immune system and promote wound healing make it a proper component for wound dressing materials. silver nanoparticles are recognized to demonstrate strong antiseptic and antimicrobial activity; thus, it also could be considered in the development of products for wound healing. combining propolis and silver nanoparticles can result in improved characteristic ...201626981531
investigation of equilibrium and kinetics of cr(vi) adsorption by dried bacillus cereus using response surface methodology.in this study, response surface methodology (rsm) based on three-variable-five-level central composite rotatable design was used to analyze the effects of combined and individual operating parameters (biomass dose, initial concentration of cr(vi) and ph) on the cr(vi) adsorption capacity of dried bacillus cereus. a quadratic polynomial equation was obtained to predict the adsorbed cr(vi) amount. analysis of variance showed that the effect of biomass dose was the key factor in the removal of cr(v ...201626877045
the pulsed light inactivation of veterinary relevant microbial biofilms and the use of a rtpcr assay to detect parasite species within biofilm structures.the presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. this study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means ...201626862516
discovery and characterization of bicereucin, an unusual d-amino acid-containing mixed two-component lantibiotic.lantibiotics are a group of ribosomally synthesized and post-translationally modified peptides (ripps) exhibiting antimicrobial activity. they are characterized by the presence of the thioether-containing bisamino acids lanthionine and methyllanthionine. here, we report a two-component lantibiotic from bacillus cereus sj1 with unusual structural features that we named bicereucin. unlike all previous two-component lantibiotics, only one of the two peptides of bicereucin contains a lanthionine. th ...201627074593
rhizosphere of avicennia marina (forsk.) vierh. as a landmark for polythene degrading bacteria.due to high durability, cheap cost, and ease of manufacture, 311 million tons of plastic-based products are manufactured around the globe per annum. the slow/least rate of plastic degradation leads to generation of million tons of plastic waste per annum, which is of great environmental concern. of the total plastic waste generated, polythene shared about 64 %. various methods are available in the literature to tackle with the plastic waste, and biodegradation is considered as the most accepted, ...201627072028
uptake and levels of the antibiotic berberine in individual dormant and germinating clostridium difficile and bacillus cereus spores as measured by laser tweezers raman spectroscopy.spores of clostridium difficile and bacillus cereus are major causes of nosocomial diarrhoea and foodborne disease. our aim was to measure the dynamics of the uptake of the antibiotic berberine by individual germinating spores and the levels of berberine accumulated in germinated spores.201626861569
microbiological analysis of rice cake processing in korea.this study was conducted to evaluate the microbial contamination in rice cake materials and products during processing and in the operation environment in nonhazard analysis [and] critical control point factories. furthermore, the environmental health of the processing facilities and the bacterial and fungal contamination on the workers' hands were investigated. pour plate methods were used for enumeration of aerobic plate count (apc), yeast and molds (ym), bacillus cereus, staphylococcus aureus ...201626735044
synthesis of sic/ag/cellulose nanocomposite and its antibacterial activity by reactive oxygen species generation.we describe the synthesis of nanocomposites, based on nanofibers of silicon carbide, silver nanoparticles, and cellulose. silver nanoparticle synthesis was achieved with chemical reduction using hydrazine by adding two different surfactants to obtain a nanocomposite with silver nanoparticles of different diameters. determination of antibacterial activity was based on respiration tests. enzymatic analysis indicates oxidative stress, and viability testing was conducted using an epifluorescence mic ...201628335299
in vitro antibacterial activity of selected medicinal plants traditionally used in vietnam against human pathogenic bacteria.medicinal plants are widely used for the treatment of different infectious diseases. infectious diseases caused by bacteria have a large impact on public health. this study aimed to determine the in vitro antibacterial activity of the medicinal plants traditionally used in vietnam against the bacterial strains associated with infectious diseases.201626819218
detection and quantification of viable bacillus cereus group species in milk by propidium monoazide quantitative real-time pcr.the bacillus cereus group includes important spore-forming bacteria that present spoilage capability and may cause foodborne diseases. these microorganisms are traditionally evaluated in food using culturing methods, which can be laborious and time-consuming, and may also fail to detect bacteria in a viable but nonculturable state. the purpose of this study was to develop a quantitative real-time pcr (qpcr) combined with a propidium monoazide (pma) treatment to analyze the contamination of uht m ...201626830746
antagonistics against pathogenic bacillus cereus in milk fermentation by lactobacillus plantarum zdy2013 and its anti-adhesion effect on caco-2 cells against pathogens.lactobacillus plantarum zdy2013 is a potential probiotic isolated from fermented bean acid. in this study, we aimed to evaluate the in vitro antimicrobial activity of this organism against bacillus cereus in milk fermentation, the antiadhesion ability on intestinal epithelial cells, as well as its ability to abrogate the cytotoxic effect and expression levels of genes. we found no antimicrobial activity produced by l. plantarum once the ph was adjusted to 6.0 and 7.0. the ph decreased continuous ...201626830743
isolation and characterization of bacterial endophytes of curcuma longa l.fourteen endophytic bacterial isolates were isolated from the rhizome of curcuma longa l. were characterized on the basis of morphology, biochemical characteristics and 16s rrna gene sequence analysis. the isolates were identified to six strains namely bacillus cereus (ecl1), bacillus thuringiensis (ecl2), bacillus sp. (ecl3), bacillus pumilis (ecl4), pseudomonas putida (ecl5), and clavibacter michiganensis (ecl6). all the strains produced iaa and solubilized phosphate and only two strains produ ...201628330130
production of γ-cyclodextrin by bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system.aqueous two-phase system (atps) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. extractive bioconversion of gamma-cyclodextrin (γ-cd) from soluble starch with cyclodextrin glycosyltransferase (cgtase, ec 2.4.1.19) enzyme derived from bacillus cereus was evaluated using polyethylene glycol (peg)/potassium phosphate based on atps. the optimum condition was attained in the atps constituted of 30.0% (w/w) peg 3000 g/mol and 7. ...201626702953
comparative elimination of dimethyl disulfide by maifanite and ceramic-packed biotrickling filters and their response to microbial community.unpleasant odor emissions have traditionally occupied an important role in environmental concern. in this paper, twin biotrickling filters (btfs) packed with different packing materials, seeded with bacillus cereus gigan2, were successfully constructed to purify gaseous dimethyl disulfide (dmds). the maifanite-packed btf showed superior biodegradation capability to the ceramic-packed counterpart in terms of removal efficiency and elimination capacity under similar conditions. at an empty bed res ...201626702514
isolation and characterization of spore-forming bacilli (sfb) from shepherd's purse (capsella bursa-pastoris).shepherd's purse (capsella bursa-pastoris), native to europe, is commonly consumed fresh and sometimes inadequately washed before consumption in korea. the objective of this study was to characterize isolates of spore-forming bacilli (sfb) in samples of fresh shepherd's purse. three genera were identified: bacillus (9 species), paenibacillus (3 species), and brevibacillus (1 species). none of the genes of the hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) complexes, or of the emetic toxin ...201626822957
antibacterial and synergistic activity of pentacyclic triterpenoids isolated from alstonia scholaris.(1) background: alstonia scholaris (apocynaceae) is an important medicinal plant that has been historically used in "dai" ethnopharmacy to treat infectious diseases in china. although various pharmacological activities have been reported, the antimicrobial constitutes of a. scholaris have not yet been identified. the objective of this study is to evaluate the antibacterial constitutes from the leaf extract of a. scholaris and to assess the synergistic effects of isolated compounds with antibioti ...201626821000
in vitro cytotoxicity and antibacterial activity of silver-coated electrospun polycaprolactone/gelatine nanofibrous scaffolds.the development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. in this study, polycaprolactone (pcl) and pcl/gelatine (ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. nanofibrous scaffolds were coated with silver (ag) in different concentrations of silver nitrate (agno3) aqueous solution (1.25, 2.5, 5, and 10 %) by usi ...201628330282
detection of viable enterotoxin-producing bacillus cereus and analysis of toxigenicity from ready-to-eat foods and infant formula milk powder by multiplex pcr.bacillus cereus is responsible for several outbreaks of foodborne diseases due to its emetic toxin and enterotoxin. enterotoxins, cytotoxin k (cytk), nonhemolytic enterotoxin (nhe), and hemolysin bl (hbl), have been recorded in several diarrheal cases due to food poisoning from b. cereus. the objective of this study was to develop a rapid and accurate method that combines multiplex pcr with propidium monoazide to selectively detect viable cells of enterotoxin-producing b. cereus in milk powder, ...201626686715
evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk.lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ...201626805974
characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food.a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ...201626803269
diversity of sugar acceptor of glycosyltransferase 1 from bacillus cereus and its application for glucoside synthesis.glycosyltransferase 1 from bacillus cereus (bcgt1) catalyzes the transfer of a glucosyl moiety from uridine diphosphate glucose (udp-glucose) to various acceptors; it was expressed and characterized. the specificity of acceptors was found to be broad: more than 20 compounds classified into o-, s-, and n-linkage glucosides can be prepared with bcgt1 catalysis. based on this work, we conclude that the corresponding acceptors of these compounds must possess the following features: (1) the acceptors ...201626795959
α,β-dibromochalcone derivatives--synthesis and antimicrobial activity.to obtain some chalcones and their dibrominated analogues and to evaluate their antimicrobial potential.201626793867
fabrication of an electrochemical dna-based biosensor for bacillus cereus detection in milk and infant formula.this paper describes fabrication of a dna-based au-nanoparticle modified pencil graphite electrode (pge) biosensor for detection of bacillus cereus, causative agent of two types of food-borne disease, i.e., emetic and diarrheal syndrome. the sensing element of the biosensor was comprised of gold nanoparticles (gnps) self-assembled with single-stranded dna (ssdna) of nhea gene immobilized with thiol linker on the gnps modified pge. the size, shape and dispersion of the gnps were characterized by ...201626896793
bacillus licheniformis trehalose-6-phosphate hydrolase structures suggest keys to substrate specificity.trehalose-6-phosphate hydrolase (trea) belongs to glycoside hydrolase family 13 (gh13) and catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to yield glucose and glucose 6-phosphate. the products of this reaction can be further metabolized by the energy-generating glycolytic pathway. here, crystal structures of bacillus licheniformis trea (bltrea) and its r201q mutant complexed with p-nitrophenyl-α-d-glucopyranoside (r201q-ppng) are presented at 2.0 and 2.05 å resolution, respectively. the ...201626894535
antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens.the marine environment is a proven source of structurally complex and biologically active compounds. in this study, the antimicrobial effects of a small collection of marine-derived extracts and isolates, were evaluated against 5 foodborne pathogens using a broth dilution assay. results demonstrated that algal extracts from padina and ulva species and cyanobacterial compounds antillatoxin b, laxaphycins a, b and b3, isomalyngamide a, and malyngamides c, i and j showed antimicrobial activity agai ...201626775951
genomic characterization and comparison of seven myoviridae bacteriophage infecting bacillus thuringiensis.bacillus thuringiensis kurstaki, a bacterium that is a source of biopesticides and a safe simulant for pathogenic bacillus species, was used to isolate seven unique bacteriophages. the phage genomes were sequenced and ranged in size from 158,100 to 163,019 bp encoding 290-299 genes, and the gc content of ~38% was similar to that of the host bacterium. all phages had terminal repeats 2-3 kb long. three of the phages encoded trnas and three contained a self-splicing intron in the dna polymerase ge ...201626773385
isolation, purification and characterization of antimicrobial protein from seedlings of bauhinia purpurea l.a novel antimicrobial protein was purified from the seedlings of bauhinia purpurea by sequential procedures entailing ammonium sulfate precipitation, cation exchange chromatography, preparative native-page and a yield of 2.7% was obtained from the crude extract. the purified antimicrobial protein appeared as a single protein band on sds-page with the molecular mass of 20.9 kda. purified antimicrobial protein exhibited a potent antimicrobial activity against both gram-positive and gram-negative b ...201626780468
butanol production under microaerobic conditions with a symbiotic system of clostridium acetobutylicum and bacillus cereus.one major problem of abe (acetone, butanol and ethanol) fermentation is high oxygen sensitivity of clostridium acetobutylicum. currently, no single strain has been isolated or genetically engineered to produce butanol effectively under aerobic conditions. in our previous work, a symbiotic system tsh06 has been developed successfully by our group, and two strains, c. acetobutylicum tsh1 and bacillus cereus tsh2, were isolated from tsh06.201626762531
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