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combination effect of cefuroxime and levofloxacin against bacteria isolated from the healthy conjunctival sac and endophthalmitis cases using a fractional inhibitory concentration index.intracameral cefuroxime (cxm) and antibiotic eye drops such as fluoroquinolone are widely used for preventing endophthalmitis after cataract surgery. however, few reports are available regarding their interactions. this study was conducted to examine the in vitro interaction of cxm and levofloxacin (lvfx) against bacterial isolates from the healthy conjunctival sac before cataract surgery and from endophthalmitis cases.201727906556
the pathogenic potential of proteus mirabilis is enhanced by other uropathogens during polymicrobial urinary tract infection.urinary catheter use is prevalent in health care settings, and polymicrobial colonization by urease-positive organisms, such as proteus mirabilis and providencia stuartii, commonly occurs with long-term catheterization. we previously demonstrated that coinfection with p. mirabilis and p. stuartii increased overall urease activity in vitro and disease severity in a model of urinary tract infection (uti). in this study, we expanded these findings to a murine model of catheter-associated uti (cauti ...201727895127
randomized clinical trial of preoperative skin antisepsis with chlorhexidine gluconate or povidone-iodine.skin antiseptic agents are used to prevent surgical-site infection (ssi); few trials have reported the superiority of any specific agent in clean-contaminated abdominal surgery. this rct was designed to compare the effectiveness of chlorhexidine gluconate and povidone-iodine.201727879993
synthesis of peptidoglycan fragments from enterococcus faecalis with fmoc-strategy for glycan elongation.peptidoglycan (pgn) is an essential structural component of the bacterial cell wall conferring cell shape, which can be recognized by host-recognition proteins and receptors as well as bacterial surface proteins. in this work, the pgn partial structures from enterococcus faecalis that contain a tetrasaccharide and an octasaccharide with a unique heptapeptide were synthesized via an fmoc-strategy for elongation of the glycan chains. namely, a 4'-o-fmoc-protected disaccharide was utilized as the k ...201727868361
most probable number - loop mediated isothermal amplification (mpn-lamp) for quantifying waterborne pathogens in <25min.we are reporting a most probable number approach integrated to loop mediated isothermal technique (mpn-lamp) focusing on gram-negative escherichia coli and gram-positive enterococcus faecalis bacterial cells without nucleic acids extraction. lamp assays for uida from e. coli and gele from e. faecalis were successfully performed directly on cells up to single digit concentration using a commercial real time pcr instrument. threshold time values of lamp assays of bacterial cells, heat treated bact ...201727856278
crip1, a novel immune-related protein, activated by enterococcus faecalis in porcine gastrointestinal epithelial cells.cysteine-rich intestinal protein 1 (crip1) is an important transcriptional regulation factor during the tumor development. although it was largely studied in the human or mouse, no report has provided functional evidence for it in the swine. to date, the real sequence of porcine crip1 (pocrip1) was also still unknown. in this study, clear characteristics for the pocrip1 were represented. a 552bp pocrip1 cdna was obtained from porcine brain tissue using real time reverse transcriptase pcr. the po ...201727836662
antimicrobial influence of different root canal filling techniques in experimentally infected human root canals.the aim of the present study was to evaluate the antimicrobial influence of different root canal filling techniques using gutta-percha and an epoxy resin-based sealer in experimentally infected root canals of extracted human teeth.201727834415
evaluation of bd max staph sr assay for differentiating between staphylococcus aureus and coagulase-negative staphylococci and determining methicillin resistance directly from positive blood cultures.we evaluated the performance of the bd max staphsr assay (sr assay; bd, usa) for direct detection of staphylococcus aureus and methicillin resistance not only in s. aureus but also in coagulase-negative staphylococci (cns) from positive blood cultures.201727834064
antimicrobial photodynamic therapy using indocyanine green and near-infrared diode laser in reducing entrerococcus faecalis.the use of antimicrobial photodynamic therapy (apdt) has been suggested as an adjuvant method to eliminate facultative bacteria during root canal disinfection. the purpose of this preliminary in vitro study was to determine whether the light-activated antimicrobial agent, indocyanine green (icg), could be used as photosensitizer and kill enterococcus faecalis strain under planktonic conditions when irradiated with near-infared (nir) diode laser emitting in 810nm wavelength.201727816623
investigation to test potential stereolithography materials for development of an in vitro root canal model.the aims were to compare the physico-chemical properties (zeta-potential, wettability, surface free energy) of stereolithography materials (stl) (photopolymer, accura) to dentine and to evaluate the potential of each material to develop enterococcus faecalis biofilm on their respective surfaces. eighteen samples of each test material (photopolymer, accura, dentine) were employed (total n = 54) and sectioned to 1 mm squares (5 mm x 5 mm) (n = 15) or ground into a powder to measure zeta-potential ...201727813213
exopolysaccharide dispelled by calcium hydroxide with volatile vehicles related to bactericidal effect for root canal medication.enterococcus faecalis is the dominant microbial species responsible for persistent apical periodontitis with ability to deeply penetrate into the dentin. exopolysaccharides (eps) contribute to the pathogenicity and antibiotic resistance of e. faecalis. our aim was to investigate the antimicrobial activity of calcium hydroxide (ch), camphorated parachlorophenol (cmcp), and chlorhexidine (chx) against e. faecalis in dentinal tubules.201727812619
biofilm behavior on sulfonated poly(ether-ether-ketone) (speek).poly(ether-ether-ketone) (peek) has also shown to be very attractive for incorporating therapeutic compounds thanks to a sulfonation process which modifies the material structure resulting in a sulfonated-peek (speek). concerning biomedical applications, the objective of this work was to evaluate the influence of different sulfonation degree of speek on the biofilm growth. peek samples were functionalized by using sulphuric acid (98%) and then dissolved into dimethyl-sulfoxide. a dip coating tec ...201727770916
intratubular disinfection with tri-antibiotic and calcium hydroxide pastes.the aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes.201727866468
effectiveness of final decontamination protocols against enterococcus faecalis and its influence on bond strength of filling material to root canal dentin.the aim of this study was to evaluate the effectiveness of final decontamination protocols against enterococcus faecalis and their influence on bond strength of filling material to root canal dentin.201727866000
triterpene glycosides from the aerial parts of gouania longipetala.six previously undescribed triterpenoid saponins, gouaniaside i-vi, were isolated from the aerial parts of gouania longipetala hemsl. (rhamnaceae), in addition to four known triterpenes. the structure elucidation of these compounds was based on analyses of spectroscopic data including 1d- and 2d-nmr and hr-esi-ms techniques. the inhibitory activity of isolated compounds against promyelocytic leukemia hl60 and human erythromyeloblastoid leukemia k562 cell lines was evaluated and jujuboside i exhi ...201727865442
the clinical, radiological, microbiological, and molecular profile of the skin-penetration site of transfemoral amputees treated with bone-anchored prostheses.the breach of the skin barrier is a critical issue associated with the treatment of individuals with transfemoral amputation (tfa) using osseointegrated, percutaneous titanium implants. thirty tfa patients scheduled for abutment exchange or removal were consecutively enrolled. the aims were to determine the macroscopic skin signs, the presence of bacteria and the gene expression in abutment-adherent cells and to conduct correlative and comparative analyses between the different parameters. redne ...201727750392
method for bacterial growth and ammonia production and effect of inhibitory substances in disposable absorbent hygiene products.the purpose of this study was to evaluate a pragmatic laboratory method to provide a technique for developing incontinence products better able to reduce malodor when used in the clinical setting.201727749743
abnormal vaginal flora in symptomatic non-pregnant and pregnant women in a greek hospital: a prospective study.bacterial vaginosis (bv), candidiasis, and trichomoniasis were the three established types of vaginal conditions until aerobic vaginitis (av) was defined in the early 2000s. we sought to study the prevalence of abnormal vaginal flora (avf) with inflammation in our hospital and to correlate it with av. we prospectively collected vaginal smear specimens originated from symptomatic women who were examined at iaso obstetrics, gynecology and children's hospital of athens from april 2014 until septemb ...201727738857
prgu: a suppressor of sex pheromone toxicity in enterococcus faecalis.upon sensing of the peptide pheromone ccf10, enterococcus faecalis cells carrying pcf10 produce three surface adhesins (prga, prgb or aggregation substance, prgc) and the prg/pcf type iv secretion system and, in turn, conjugatively transfer the plasmid at high frequencies to recipient cells. here, we report that ccf10 induction is highly toxic to cells sustaining a deletion of prgu, a small orf located immediately downstream of prgb on pcf10. upon pheromone exposure, these cells overproduce the ...201727785854
vancomycin-resistant enterococci in neonatal stool as a cause of septicemia: challenges for infection control practices.the increasing reports of vancomycin-resistant enterococci (vre) as a cause of neonatal septicemia are of recent interest. however, in majority of the cases, the source of vre could not be located. as a consequence, the real importance of vre and its control measures is undermined. herein, we report a case of neonatal septicemia due to vre (enterococcus faecalis) of vana genotype with vre carriage in stool of the neonates as a possible source of sepsis. the report put forwards some lacunae in th ...201727721296
effect of chitosan-ethylenediamine tetraacetic acid on enterococcus faecalis dentinal biofilm and smear layer removal.the objective of the study was to evaluate the effectiveness of chitosan and chitosan-ethylenediamine tetraacetic acid (edta) (3:1,1:1,1:3) in comparison with 5.2% sodium hypochlorite (naocl) in disinfecting enterococcus faecalis biofilm on root canal dentin and in the removal of smear layer with minimal erosion.201727656070
vitality of enterococcus faecalis inside dentinal tubules after five root canal disinfection methods.to compare the vitality of enterococcus faecalis within dentinal tubules after subjected to five root canal disinfection methods.201727656064
long-term clonal dynamics of enterococcus faecium strains causing bloodstream infections (1995-2015) in spain.to investigate the population structure of enterococcus faecium causing bloodstream infections (bsis) in a tertiary spanish hospital with low glycopeptide resistance, and to enhance our knowledge of the dynamics of emergence and spread of high-risk clonal complexes.201727655856
differences in the chemical composition of enterococcus faecalis biofilm under conditions of starvation and alkalinity.abstact this study aimed to investigate the dynamic changes that occur in the chemical composition of an enterococcus faecalis (e. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the e. faecalis biofilm to an extreme environment. this study established an in vitro e. faecalis biofilm model under starvation and in an alkaline environment. during the formation of the biofilm, the ph val ...201727689418
enterococcus faecalis and dental implants.enterococcus faecalis appears in many tooth root infections and is not eliminated by root canal therapy. it can reside in tooth root canals and the surrounding bone. this species may vegetate in bone after extraction of an infected tooth and colonize a dental implant after placement in the healed site. a colonization may cause fixture loss or marginal bone loss. these colonizations are generally multibacterial and pathogenic properties can be shared via plasmids. however, e faecalis is not detec ...201727700696
urinary microbiome of kidney transplant patients reveals dysbiosis with potential for antibiotic resistance.recent studies have established that a complex community of microbes colonize the human urinary tract; however, their role in kidney transplant patients treated with prophylactic antibiotics remains poorly investigated. our aim was to investigate the urinary microbiome of kidney transplant recipients. urine samples from 21 patients after kidney transplantation and 8 healthy controls were collected. all patients received prophylactic treatment with the antibiotic combination trimethoprim-sulfamet ...201727669488
probiotic enterococcus faecalis symbioflor(®) down regulates virulence genes of ehec in vitro and decrease pathogenicity in a caenorhabditis elegans model.enterohemorrhagic e. coli o157:h7 (ehec) shorten the lifespan of caenorhabditis elegans compared to avirulent bacteria. co-feeding ehec with enterococcus faecalis symbioflor(®) significantly increased the worms' lifespan. the transcriptome of ehec grown in vitro with or without symbioflor(®) was analyzed using rna-seq. the analysis revealed downregulation of several virulence-associated genes in the presence of symbioflor(®), including virulence key genes (e.g., lee, flagellum, quorum-sensing). ...201727655246
(13)c-metabolic flux analysis for mevalonate-producing strain of escherichia coli.mevalonate (mva) is used to produce various useful products such as drugs, cosmetics and food additives. an mva-producing strain of escherichia coli (engineered) was constructed by introducing mvaes genes from enterococcus faecalis. the engineered strain produced 1.84 mmol/gdcw/h yielding 22% (c-mol/c-mol) of mva from glucose in the aerobic exponential growth phase. the mass balance analysis revealed that the mva yield of the engineered strain was close to the upper limit at the biomass yield. s ...201727570223
indole production provides limited benefit to escherichia coli during co-culture with enterococcus faecalis.escherichia coli lives in the gastrointestinal tract and elsewhere, where it coexists within a mixed population. indole production enables e. coli to grow with other gram-negative bacteria as indole inhibits n-acyl-homoserine lactone (ahl) quorum regulation. we investigated whether e. coli indole production enhanced competition with gram-positive enterococcus faecalis, wherein quorum signaling is mediated by small peptides. during planktonic co-culture with e. faecalis, the fitness and populatio ...201727638396
comparison of the efficiency of rose bengal and methylene blue as photosensitizers in photodynamic therapy techniques for enterococcus faecalis inactivation.the aim of this in vitro study was to compare the efficiency of a photodynamic therapy (pdt) technique employing rose bengal (rb) and methylene blue (mb) as photosensitizers (pss) to reduce the viability of enterococcus faecalis, a well-known pathogen found in root canal systems. currently, in several clinical applications, including in the field of endodontics, mb is employed in association with a red laser source for the photoinactivation of pathogenic bacteria.201727617768
breaking down polystyrene through the application of a two-step thermal degradation and bacterial method to produce usable byproducts.in this work, the objective is to examine a novel approach on the biodegradability of polystyrene is examined. a two-step method for the degradation of polystyrene to chemically useful products has been devised. initially, polystyrene is liquefied via a thermal degradation procedure predominantly into styrene monomers at low temperatures and left to cool down. application of microorganisms in a second step targets the breaking of the polymer into smaller organic molecules. microorganisms tested ...201727440221
in vitro comparison of antibacterial properties of bioceramic-based sealer, resin-based sealer and zinc oxide eugenol based sealer and two mineral trioxide aggregates.our goal of the study was to evaluate the antibacterial properties of endodontic sealers against the e. faecalis.201727403055
rapid assessment of resistance to antibiotic inhibitors of protein synthesis in the gram-positive pathogens, enterococcus faecalis and streptococcus pneumoniae, based on evaluation of the lytic response.a novel assay for rapid determination of resistance to antibiotic inhibitors of protein synthesis was developed for the gram-positive pathogens, enterococcus faecalis and streptococcus pneumoniae. to this purpose, a lytic response was obtained by a brief incubation with lysozyme or a mixture of lysozyme, triton x-100, and edta for e. faecalis (n = 82) and s. pneumoniae (n = 51), respectively. lysis was quantified by visualizing the released nucleoids. antibiotic-susceptible bacteria treated with ...201727383851
comparative evaluation of antimicrobial effect of herbal root canal irrigants (morinda citrifolia, azadirachta indica, aloe vera) with sodium hypochlorite: an in vitro study.successful root canal treatment involves the complete elimination of microorganism from the root canal and the three-dimensional obturation of the canal space. enterococcus faecalis is the most commonly found bacteria in failed root canal. chemical irrigation of canals along with biomechanical preparation helps in the elimination of microorganisms. the present study was aimed to evaluate the antimicrobial effect of herbal root canal irrigants (morinda citrifolia, azadirachta indica extract, aloe ...201727382533
synthesis and biological evaluation of lipophilic teicoplanin pseudoaglycon derivatives containing a substituted triazole function.a series of lipophilic teicoplanin pseudoaglycon derivatives, including alkyl-, aryl-, calixarene- and protected sugar-containing conjugates, were prepared using azide-alkyne click chemistry. out of the conditions applied, the cuso4-ascorbate reagent system proved to be more efficient than the cu(i)i-et3n-mediated reaction. some of the new compounds have high in vitro activity against glycopeptide-resistant gram-positive bacteria, including vana-positive enterococcus faecalis. a few of them also ...201727353163
characteristics of vancomycin-resistant enterococcus strains in the west balkans: a first report.vancomycin-resistant enterococci are among the major causes of nosocomial infections and represent a growing problem in many european countries. among the most common enterococcal isolates, enterococcus faecium is considered to be the reservoir of vana and vanb-mediated resistance to glycopeptides. enterococci with vana-mediated resistance can transfer resistance genes to other enterococci and gram-positive bacteria. hence, monitoring and surveillance of vancomycin-resistant enterococci (vres) a ...201727351410
multi-drug-resistant enterococcus faecalis among egyptian patients with urinary tract infection.the prevalence of enterococcus faecalis (e. faecalis) infections among egyptians with urinary tract infection (uti), their antimicrobial susceptibility and mechanisms of resistance are under investigated. in this study, 300 urine samples were collected from uti patients to identify e. faecalis. antimicrobial susceptibility to 18 antimicrobial agents was tested. the presence of aac(6)-ie-aph(2)ia, erm(b) and mef(a/e) genes was examined by pcr. fifty-seven (19%) isolates were identified as e. faec ...201727351108
in vitro evaluation of the antimicrobial efficacy of chitosan and other endodontic irrigants against enterococcus faecalis.the success of endodontic treatment is directly enhanced by elimination of microorganisms in infected root canals. recently, chitosan, a natural, nontoxic biopolymer, has been introduced as an irrigant that has the capacity to remove the smear layer. the antimicrobial properties of chitosan as an endodontic irrigant have not yet been explored. the purpose of this study was to compare the antimicrobial efficacy of biopure mtad, 0.2% chitosan, 1% chitosan, 2% chlorhexidine gluconate, and 3% sodium ...201727599284
probiotic properties and adsorption of enterococcus faecalis psct3-7 to vermiculite.the probiotic properties of enterococcus (e.) faecalis psct3-7, a new strain isolated from the intestines of pigs fed dietary fiber containing 50% sawdust, were investigated. e. faecalis psct3-7 tolerated a ph range of 3 to 8 and 0.3% bile salts, and it inhibited the growth of salmonella typhimurium in a concentration-dependent manner. in addition, e. faecalis showed resistance to several antibacterial agents. vermiculite, a nutrient and microbial carrier, increased the bile tolerance of the str ...201727456777
the effect of berberine hydrochloride on enterococcus faecalis biofilm formation and dispersion in vitro.enterococcus faecalis (e. faecalis) is one of the major causes of biofilm infections. berberine hydrochloride (bbh) has diverse pharmacological effects; however, the effects and mechanisms of bbh on e. faecalis biofilm formation and dispersion have not been reported. in this study, 99 clinical isolates from the urine samples of patients with urinary tract infections (utis) were collected and identified. ten strains of e. faecalis with biofilm formation ability were studied. bbh inhibited e. faec ...201727242142
the prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in inner mongolia, china.this study highlights the prevalence of aminoglycoside-modifying enzyme genes and virulence determinants among clinical enterococci with high-level aminoglycoside resistance in inner mongolia, china. screening for high-level aminoglycoside resistance against 117 enterococcal clinical isolates was performed using the agar-screening method. out of the 117 enterococcal isolates, 46 were selected for further detection and determination of the distribution of aminoglycoside-modifying enzyme-encoding ...201727268115
to defend or not to defend: that's the question.enterococcus faecalis is an opportunistic pathogen and is one of the leading causes of nosocomial infections. e. faecalis harbors a number of antibiotic resistance genes, and most of these are present on mobile genetic elements (mges) that can be disseminated within the species, as well as to other members of the human microflora. in an article by price and colleagues [v. j. price et al., msphere 1(3):e00064-16, 2016, http://dx.doi.org/10.1128/msphere.00064-16], the authors demonstrated how e. f ...201727306929
crispr-cas and restriction-modification act additively against conjugative antibiotic resistance plasmid transfer in enterococcus faecalis.enterococcus faecalis is an opportunistic pathogen and a leading cause of nosocomial infections. conjugative pheromone-responsive plasmids are narrow-host-range mobile genetic elements (mges) that are rapid disseminators of antibiotic resistance in the faecalis species. clustered regularly interspaced short palindromic repeat (crispr)-cas and restriction-modification confer acquired and innate immunity, respectively, against mge acquisition in bacteria. most multidrug-resistant e. faecalis isola ...201727303749
detection of vancomycin resistance in enterococcus species isolated from clinical samples and feces of colonized patients by phenotypic and genotypic methods.the aim of this study was to find out the clinical correlation between the presence of vancomycin-resistant genes (van a and van b) and their expression as detected by phenotypic tests in colonized patients and in clinical isolates.201727166038
efficacy of calcium oxide and calcium hydroxide nanoparticles on the elimination of enterococcus faecalis in human root dentin.the objective of this study was to compare the antibacterial effect of calcium oxide nanoparticles (conps) and calcium hydroxide nanoparticles (chnps) against enterococcus faecalis in a dentinal block model.201727129586
effect of laser-activated irrigation on biofilms in artificial root canals.to evaluate the antimicrobial effect of laser-activated irrigation (lai) on biofilms formed in simulated root canals.201727014832
molecular characterization of virulence genes in vancomycin-resistant and vancomycin-sensitive enterococci.the aim of this study was to find out the correlation between presence of virulence (gelatinase [gel e], enterococcal surface protein [esp], cytolysin a [cyl a], hyaluronidase [hyl], and aggregation substance [asa1]) and vancomycin-resistant genes (van a and van b) in enterococci, with their phenotypic expression.201727013840
pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections.it has been proposed that inactivated probiotics may modulate the host immune system and contribute to mitigation of viral infections. this study demonstrated that administration of heat-killed enterococcus faecalis, a widely used probiotic, can protect host animals against viral infections. the influenza-mediated morbidity and lung inflammation in e. faecalis-treated mice decreased significantly compared with those of the control mice. furthermore, we found that the protection is associated wit ...201727072606
light and temperature effects on bioactivity in diatoms.isolates of five pelagic north atlantic marine diatoms (bacillariophyceae): attheya longicornis, chaetoceros socialis, chaetoceros furcellatus, skeletonema marinoi and porosira glacialis were cultivated in large photobioreactors at two light and two temperature regimes to test if this affected bioactivity. we screened for bioactivity in assays representing five different therapeutic areas: diabetes ii (ptp1b), cancer (melanoma cells, a2058), anti-oxidants (frap), immunomodulation (tnfa) and anti ...201727057087
lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial.michalickova, dm, kostic-vucicevic, mm, vukasinovic-vesic, md, stojmenovic, tb, dikic, nv, andjelkovic, ms, djordjevic, bi, tanaskovic, bp, and minic, rd. lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial. j strength cond res 31(1): 62-70, 2017-to test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. thirty a ...201727100317
antimicrobial effect of three disinfecting agents on resilon cones and their effect on surface topography: an in vitro study.the objective of this study was to evaluate the effectiveness of 5.25% sodium hypochlorite (naocl), 2% chlorhexidine (chx), and 2% peracetic acid (paa) in disinfecting resilon cones and to evaluate topographical changes microscopically under scanning electron microscope (sem) after rapid chemical disinfection.201727099418
community acquired enterococcal urinary tract infections and antibiotic resistance profile in north india.urinary tract infections (utis) remain a major problem both in hospitalized and outdoor patients. multidrug-resistant enterococci are emerging as a major nosocomial pathogen with increasing frequency. however, the incidence of community-acquired enterococcal infections and species prevalent in india is not thoroughly investigated.201727013814
influence of temperature on the antibacterial activity of sodium hypochlorite.the aim of this study was to compare the antimicrobial activity of 5.25% naocl, hypoclean and chlor-xtra at 20 °c and 45 °c in bovine root dentin. one-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with enterococcus faecalis. the specimens were divided into the following groups: 1. 5.25% naocl 20 °c; 2. hypoclean 20 °c; 3. chlor-xtra 20 °c; 4. 5.25% % naocl 45 °c; 5. hypoclean 45 °c; 6. chlor-xtra 45 °c; 7. positive control; 8. negative control ...201727007342
2-hydroxyisocaproic acid is bactericidal in human dental root canals ex vivo.to compare the activity of 2-hydroxyisocaproic acid (hica), calcium hydroxide (ca(oh)2 ) and chlorhexidine digluconate (chg) against enterococcus faecalis t-75359 (root canal isolate) in the root canals of extracted human teeth.201727004733
effectiveness of n-acetyl cysteine, 2% chlorhexidine, and their combination as intracanal medicaments on enterococcus faecalis biofilm.the purpose of this study was to evaluate the antibacterial efficacies of 2% chlorhexidine (chx), n-acetyl cysteine (nac) and assess their synergistic or antagonist action as intracanal medicament.201726957787
comparative antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), mtad, and 2.5% sodium hypochlorite against enterococcus faecalis: an in vitro study.the objective of this study was to evaluate the antimicrobial efficacy of herbal alternatives (emblica officinalis, psidium guajava), biopure mtad, and 2.5% sodium hypochlorite against enterococcus faecalis.201726937369
in vitro antimicrobial evaluation of toothpastes with natural compounds.this in vitro study evaluated the antimicrobial effects of commercial toothpastes containing natural compounds.201726929699
a new mechanism of post-transfer editing by aminoacyl-trna synthetases: catalysis of hydrolytic reaction by bacterial-type prolyl-trna synthetase.aminoacyl trna synthetases are enzymes that specifically attach amino acids to cognate trnas for use in the ribosomal stage of translation. for many aminoacyl trna synthetases, the required level of amino acid specificity is achieved either by specific hydrolysis of misactivated aminoacyl-adenylate intermediate (pre-transfer editing) or by hydrolysis of the mischarged aminoacyl-trna (post-transfer editing). to investigate the mechanism of post-transfer editing of alanine by prolyl-trna synthetas ...201726886480
enhancing the antibacterial activity of the gold standard intracanal medicament with incorporation of silver zeolite: an in vitro study.enterococcus faecalis is a persistent organism that plays a major role in the etiology of persistent periradicular lesions after root canal treatment has been associated with different forms of periradicular disease including primary endodontic infections and persistent infections. the present study compares the antibacterial activities of calcium hydroxide, calcium hydroxide mixed with silver zeolite, and calcium hydroxide mixed with 2% chlorhexidine against e. faecalis using direct contact tes ...201727011937
antimicrobial effect of an oxazolidinone, lantibiotic and calcium hydroxide against enterococcus faecalis biofilm: an in vitro study.the aim was to evaluate and compare the antimicrobial efficacy of an oxazolidinone (linezolid [lz]), lantibiotic (nisin), and calcium hydroxide against enterococcus faecalis biofilm formed on tooth substrate after 2 and 7 days.201726752879
antimicrobial efficacy of different root canal sealers by using real-time polymerase chain reaction: an ex vivo study.root canal sealers help to minimize leakage, provides antimicrobial activity to reduce the possibility of residual bacteria, and to resolve periapical lesion.201726752843
quantitative evaluation of apical extrusion of intracanal bacteria using rotary protaper, k3xf, twisted and hand k-file system: an ex vivo study.the aim of this study was to evaluate the number of intracanal bacteria extruded apically during root canal preparation using rotary protaper, k3xf, twisted, and hand k-file system.201726481889
effect of passive ultrasonic irrigation on enterococcus faecalis from root canals: an ex vivo study.endodontic irrigation aims to clean and disinfect the root canal system. passive ultrasonic irrigation (pui) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. the aim of this study was to evaluate, ex vivo, the effectiveness of pui in eliminating enterococcus faecalis from root canals. seventy-five extracted human single-root teeth were used. after root canal preparation, specimens were inoculated with e. faecalis and incubated at 37 °c for 21 da ...201726312969
biofilm forming capacity of enterococcus faecalis on gutta-percha points treated with four disinfectants using confocal scanning laser microscope: an in vitro study.the aim of this study was to evaluate and compare the in vitro biofilm forming capacity of enterococcus faecalis on gutta-percha points disinfected with four disinfectants.201726288622
antibiotic resistance pattern of enterococci isolates from nosocomial infections in a tertiary care hospital in eastern india.resistance to commonly used antibiotics by enterococci causing nosocomial infections is of concern, which necessitates judicious, responsible and evidence-based use of antibiotics. the present study was conducted to review the prevalence and identify therapeutic options for nosocomial enterococcal infections in our tertiary care hospital.201726283837
phytochemical screening, antioxidant and antibacterial activities of extracts prepared from different tissues of schinus terebinthifolius raddi that occurs in the coast of bahia, brazil.schinus terebinthifolius is widely used in traditional medicine by brazilian quilombola and indigenous communities for treatment of several diseases. extracts from different tissues are being used to produce creams to treat cervicitis and cervicovaginitis. however, most studies are limited to the assessment of the essential oils and extracts obtained from the leaves.201726246739
reduction of enterococcus faecalis in curved root canals after various sizes and tapers of canal preparation.the aim of this study was to evaluate the reduction of enterococcus faecalis in curved root canals after various sizes and tapers of the canal preparation.201726180416
an assessment of antibacterial activity of three pulp capping materials on enterococcus faecalis by a direct contact test: an in vitro study.the aim of this in vitro study was to evaluate antimicrobial activities of three different pulp capping materials; biodentine, mineral trioxide aggregate (mta) angelus, and dycal against enterococcus faecalis and their durability with time.201726038657
bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms.the aim of the present study was to evaluate the bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400 (peg 400), and polyethylene glycol 1000 (peg 1000) against selected microorganisms in vitro.201725992336
comparison of fluid filtration and bacterial leakage techniques for evaluation of microleakage in endodontics.apical leakage assessment is a way to compare the efficiency of a filling material to seal the apical region of the tooth. many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results. the aim of this study was to compare the accuracy of fluid filtration and bacterial leakage techniques in the assessment of the apical sealing ability of mineral trioxide aggregate (mta) and calcium enriched mixtu ...201725878674
apical extrusion of enterococcus faecalis using three different rotary instrumentation techniques: an in vitro study.to compare the apical extrusion of enterococcus faecalis after instrumentation with three different ni-ti rotary instruments- an in vitro study.201725961619
drinking water quality and public health in southwestern saudi arabia: the need for a national monitoring program.the aim was to investigate the bacteriological quality of drinking water, and explore the factors involved in the knowledge of the public about the quality of drinking water in najran region, saudi arabia.201725657607
sealing ability of a novel hydrophilic vs. conventional hydrophobic obturation systems: a bacterial leakage study.comparative assessment of apical sealing ability of a novel smart-seal system, resilon, and conventional gutta-percha system using a bacterial leakage model.201725657530
antibacterial and smear layer removal capability of oregano extract solution.the aim of this in vitro study was to evaluate the antimicrobial effect of oregano extract solution (oes) against enterococcus faecalis within root canals and dentin tubules, and its effect on smear layer.201725713479
antibacterial effect of calcium hydroxide combined with chlorhexidine on enterococcus faecalis: a systematic review and meta-analysis.enterococcus faecalis (e. faecalis) is the most frequently isolated strain in failed endodontic therapy cases since it is resistant to calcium hydroxide (ch). whether a combination of ch and chlorhexidine (chx) is more effective than ch alone against e. faecalis is a matter of controversy. thus, the aim of this study was to conduct a systematic review and meta-analysis of the literature.201725466470
enterococcus faecium and enterococcus faecalis in blood of newborns with suspected nosocomial infection.enterococci are gram-positive cocci saprophyte of the human gastrointestinal tract, diners who act as opportunistic pathogens. they can cause infections in patients hospitalized for a long time or who have received multiple antibiotic therapy. enterococcus faecalis and enterococcus faecium are the most common species in human infections. to evaluate the possibility of rapid detection of these species and their occurrence in the blood of newborns with suspected nosocomial infection, blood samples ...201724553613
ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine.to assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of enterococcus faecalis in filled root canals.201723559116
comparative evaluation of antimicrobial efficacy of mtad, 3% naoci and propolis against e faecalis.the present study sought to compare the antimicrobial efficacy of 3% naocl, biopure mtad (tulsa dentsply, tulsa, ok) and brazilian ethanolic extract of propolis (eep) against enterococcus faecalis (e. faecalis).201727625552
validity of somatic cell count as indicator of pathogen-specific intramammary infections.the objective of this study was to determine whether somatic cell count (scc) was an effective test, with a sensitivity exceeding 85%, to determine species-specific bacterial infections. in addition, the relation between the scc and various udder pathogen groups was investigated. scc thresholds of greater than 200 000 cells/ml were used in quarter and greater than 150 000 cells/ml in composite milk samples. a retrospective study was conducted on a data set for 89 635 quarter and 345 467 composit ...201728470079
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...201728725151
antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi.lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. the main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e. caloplaca pusilla, protoparmeliopsis muralis and xanthoria parietina and to evaluate antibacterial and antiproliferative activities of their acetone ex ...201728592323
inactivation of bacterial biofilms by visible light-activated unmodified zno nanorods.various zinc oxide (zno) nanostructures are widely used for photocatalytic antibacterial applications. since zno possess a wide bandgap, it is believed that only uv light may efficiently assist bacterial inactivation and diverse crystal lattice modifications should be applied in order to narrow the bandgap for efficient visible light absorption. in this work we show that even unmodified zno nanorods grown by aqueous chemical growth technique were found to possess intrinsic defects which can be a ...201728628486
efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces.air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ...201728803189
physicochemical characterization of water-soluble chitosan derivatives with singlet oxygen quenching and antibacterial capabilities.new water-soluble chitosan derivatives (wsch) were obtained by maillard reaction (mr) between glucosamine (ga) with both low and medium molecular weight chitosans (ch). the wsch showed larger solubility than the respective ch, while their deacetylation degree (dd) decreased by approximately 12%. infrared spectroscopy experiments of wsch confirmed the formation of imine bonds after mr with intensified pyranose structure, and sugar molecules as polymer branches. however, a 6-times reduction of the ...201728396271
do quaternary ammonium monomers induce drug resistance in cariogenic, endodontic and periodontal bacterial species?antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (qams) would induce drug resistance in oral bacteria. the objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (dmahdm) and dimethylaminododecyl methacrylate (dmaddm) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first tim ...201728755761
the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells.six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ...201728811611
detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization.simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses.201728713514
high level bacterial contamination of secondary school students' mobile phones.while contamination of mobile phones in the hospital has been found to be common in several studies, little information about bacterial abundance on phones used in the community is available. our aim was to quantitatively determine the bacterial contamination of secondary school students' mobile phones.201728626737
induction of cryptic and bioactive metabolites through natural dietary components in an endophytic fungus colletotrichum gloeosporioides (penz.) sacc.grape skin and turmeric extracts having the major components resveratrol and curcumin, respectively, were used for the induction of cryptic and bioactive metabolites in an endophytic fungus colletotrichum gloeosporioides isolated from syzygium cumini. the increase in total amount of crude compounds in grape skin and turmeric extract treated cultures was 272.48 and 174.32%, respectively, compared to the untreated control. among six human pathogenic bacteria tested, the maximum inhibitory activity ...201728674526
use of whey as a culture medium for bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity.the use of whey as a natural culture medium to produce hydrolysates with antimicrobial from bacillus clausii is the objective of this research. the fermentation process was carried out at 25 ℃ for five days, where proteolytic activity, soluble peptides quantification, and antimicrobial activity using the disc diffusion method were determined every day. the fermented whey reached values ​​of proteolytic activity between 90 and 260 u ml(-1) and a production of soluble peptides between 100 and 2070 ...201728816527
ultraviolet-c light as a means of disinfecting anesthesia workstations.anesthesia workstations (aws) are a reservoir for pathogenic organisms potentially associated with surgical site infections. this study examined the effectiveness of the tru-d smartuvc device (tru-d llc, nashville, tn) on bioburden reduction (br) on aws.201728431851
phytochemical screening, antimicrobial and cytotoxicity studies of ethanol leaf extract of aphaniasenegalensis (sapindaceae).aphania senegalensis (sapindaceae) is commonly used in senegalese traditional medicine to treat pain, inflammation, asthenia, bacterial and fungal infections. the aim of this study was to determine the type of phytochemical constituents present in the ethanol leaf extract and its antimicrobial activity against selected bacterial and fungal pathogens.201728638876
the curious case of achromobacter eurydice, a gram-variable pleomorphic bacterium associated with european foulbrood disease in honeybees.honeybees are prone to parasite and pathogen infestations/infections due to their social colony life. bacterial pathogens in particular lead to destructive infections of the brood. european foulbrood is caused by the bacterium melissococcus plutonius in combination with several other gram-positive bacteria (achromobacter eurydice, bacillus pumilus, brevibacillus laterosporus, enterococcus faecalis, paenibacillus alvei, paenibacillus dendritiformis) involved as secondary invaders following the in ...201728634639
1,1ꞌ-carbonyldiimidazole (cdi) mediated facile synthesis, structural characterization, antimicrobial activity and in-silico studies of coumarin-3-carboxamide derivatives.despite of the availability of variety of antibacterial agents, re-emergance of pathogenic bacteria is still a serious medical concern. so, identification of new, safer, and selective antibacterial agents is the key interest in the medicinal chemistry research.201728641525
cytotoxic and antimicrobial activities of cantharellus cibarius fr. (cantarellaceae).antibacterial and cytotoxic activities of cyclohexane, dichloromethane, methanol, and aqueous extracts of cantharellus cibarius were tested. broth microdilution assay was performed against 10 bacterial strains (staphylococcus aureus, s. epidermidis, micrococcus luteus, bacillus subtilis, enterococcus feacalis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella abony), with emphasis on helicobacter pylori. methanol extract was the most active against h. pylori strains wit ...201728613963
safety evaluation of enterocin producer enterococcus sp. strains isolated from traditional turkish cheeses.the purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. enterococcus strains were isolated from 33 different cheeses. enterococcus faecium (6 strains) and enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16s rdna analyses. structural genes enta, entb, entp and entx were detected in some isolates. mu ...201728735309
isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india.a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ...201728720951
characterization of high exopolysaccharide-producing lactobacillus strains isolated from mustard pickles for potential probiotic applications.the aim of this study was to characterize high exopolysaccharide (eps)-producing lactic acid bacteria (lab) isolated from mustard pickles in taiwan for potential probiotic applications. among 39 collected lab strains, four most productive eps-producing strains were selected for further analysis. comparative analyses of 16s rdna genes rpoa and phes sequences demonstrated that these strains were members of lactobacillus plantarum-group (lpg). ncd 2, nld 4, slc 13, and nld 16 showed survival rates ...201728617525
seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium.seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ...201728733150
investigation of effect of 1,8-cineole on antimicrobial activity of chlorhexidine gluconate.chlorhexidine belongs to a group of medicines called antiseptic antibacterial agents. chlorhexidine is commonly used for the care and clean off the skin, hands, and wounds. in recent years, medicinal and aromatic plants have been used for prevention of disease, maintaining health, and improving disease in traditional and modern medicine as a medicament. according to recent research, cineole is the isolated active agent of eucalyptus oil and possesses antimicrobial activity. it was demonstrated t ...201728827963
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