Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| development and application of loop-mediated isothermal amplification assays based on its-1 for rapid detection of toxoplasma gondii in pork. | the loop-mediated isothermal amplification (lamp) assay is a novel method that rapidly amplifies dna with high specificity and sensitivity under isothermal conditions. in this study, we established a lamp assay with six primers targeting a highly conserved region of toxoplasma gondii its-1 sequence. the amplification protocol completes within 30 min under isothermal condition in a 65°c water bath while specificity tests confirmed no cross-reactivity with dna templates of neospora caninum, eimeri ... | 2015 | 25624074 |
| disposition of ceftiofur and its active metabolites in fallow deer (dama dama) following single-dose intravenous and intramuscular administration. | septicemia and foot infections associated with fusobacterium necrophorum , pasturella multocida, and streptococcus suis in captive fallow deer (dama dama) are reasonably treated with ceftiofur hydrochloride. this study describes the disposition of ceftiofur after single-dose intravenous and intramuscular administration of 3.65±0.1678 mg/kg in six female adult fallow deer using a nonrandomized crossover design and a 7-day washout period. serial blood samples were collected for 12 hr postdrug admi ... | 2015 | 26056876 |
| sialic acid-dependent interactions between influenza viruses and streptococcus suis affect the infection of porcine tracheal cells. | bacterial co-infections are a major complication in influenza-virus-induced disease in both humans and animals. either of the pathogens may induce a host response that affects the infection by the other pathogen. a unique feature in the co-infection by swine influenza viruses (siv) and streptococcus suis serotype 2 is the direct interaction between the two pathogens. it is mediated by the haemagglutinin of siv that recognizes the α2,6-linked sialic acid present in the capsular polysaccharide of ... | 2015 | 26297001 |
| dynamic virus-bacterium interactions in a porcine precision-cut lung slice coinfection model: swine influenza virus paves the way for streptococcus suis infection in a two-step process. | swine influenza virus (siv) and streptococcus suis are common pathogens of the respiratory tract in pigs, with both being associated with pneumonia. the interactions of both pathogens and their contribution to copathogenesis are only poorly understood. in the present study, we established a porcine precision-cut lung slice (pcls) coinfection model and analyzed the effects of a primary siv infection on secondary infection by s. suis at different time points. we found that siv promoted adherence, ... | 2015 | 25916988 |
| multiplex pcr and microarray for detection of swine respiratory pathogens. | porcine respiratory disease complex (prdc) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. no simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with prdc. furthermore, the detection of most of the bacterial pathogens implicated in prdc currently requires time-consuming culture-based methods that can take several days to obtain results. in this study, ... | 2015 | 26662640 |
| purification and characterization of suicin 65, a novel class i type b lantibiotic produced by streptococcus suis. | bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent streptococcus suis (serotype 2). in this study, a novel bacteriocin produced by s. suis has been identified and characterized. the producing strain s. suis 65 (serotype 2) was fo ... | 2015 | 26709705 |
| impact of an experimental prrsv and streptococcus suis coinfection on the pharmacokinetics of ceftiofur hydrochloride after intramuscular injection in pigs. | this study determined the impact of porcine reproductive and respiratory syndrome virus (prrsv) and streptococcus suis coinfection on the pharmacokinetic (pk) profile of ceftiofur hydrochloride in pigs after intramuscular (i.m.) injection. eighteen clinically normal crossbred gilts were assigned by weight into a challenge group (10 pigs) and control group (eight pigs). pigs in both groups received a single i.m. injection of ceftiofur hydrochloride (excenel rtu sterile suspension; zoetis) at a 5 ... | 2015 | 25689130 |
| diadenylate cyclase evaluation of ssdaca (ssu98_1483) in streptococcus suis serotype 2. | cyclic diadenosine monophosphate is a recently identified signaling molecule. it has been shown to play important roles in bacterial pathogenesis. ssu98_1483 (ssdaca), which is an ortholog of listeria monocytogenes daca, is a putative diadenylate cyclase in streptococcus suis serotype 2. in this study, we determined the enzymatic activity of ssdaca in vitro using high-performance liquid chromatography and mass spectrometry. our results showed that ssdaca was a diadenylate cyclase that converts a ... | 2015 | 26125899 |
| multi-etiological nature of tuberculosis-like lesions in condemned pigs at the slaughterhouse. | tuberculosis-like lesions (tbl) in pigs have been associated with microorganisms other than mycobacteria. in this work a histopathological and microbiological evaluation of tbl in pigs is shown. a total of 352 samples belonging to 171 pigs totally condemned at slaughterhouse due to generalized tbl were sampled and selected for analysis. pyogranulomatous (56.2%) and granulomatous lesions (20.2%) were observed in all analysed organs. most of the granulomas observed in both lymph nodes and lungs be ... | 2015 | 26418681 |
| synthesis and antibacterial activity of novel pleuromutilin derivatives. | in this study we describe the design, synthesis, and antibacterial activity of novel pleuromutilin analogs. a series of new compounds containing piperazine and alkylamino or arylamino groups was synthesized. the new compounds were characterized via (1)h-nmr, (13)c-nmr, fourier transform (ft)-ir and ms, and were further evaluated for their in vitro activity against seven gram-positive, and one gram-negative, pathogens. antibacterial data revealed that all compounds exhibited moderate to good anti ... | 2015 | 26133714 |
| genomic comparison between pathogenic streptococcus agalactiae isolated from nile tilapia in thailand and fish-derived st7 strains. | streptococcus agalactiae, or group b streptococcus (gbs), is a highly virulent pathogen in aquatic animals, causing huge mortalities worldwide. in thailand, the serotype ia, β-hemolytic gbs, belonging to sequence type (st) 7 of clonal complex (cc) 7, was found to be the major cause of streptococcosis outbreaks in fish farms. in this study, we performed an in silico genomic comparison, aiming to investigate the phylogenetic relationship between the pathogenic fish strains of thai st7 and other st ... | 2015 | 26455417 |
| recombination between streptococcus suis icessu32457 and streptococcus agalactiae icesa2603 yields a hybrid ice transferable to streptococcus pyogenes. | integrative conjugative elements (ices) are mobile genetic elements that reside in the chromosome but retain the ability to undergo excision and to transfer by conjugation. genes involved in drug resistance, virulence, or niche adaptation are often found among backbone genes as cargo dna. we recently characterized in streptococcus suis an ice (icessu32457) carrying resistance genes [tet(o/w/32/o), tet(40), erm(b), apha, and aade] in the 15k unstable genetic element, which is flanked by two ∼1.3k ... | 2015 | 25935120 |
| antioxidant activity and antibacterial effects on clinical isolated streptococcus suis and staphylococcus intermedius of extracts from several parts of cladogynos orientalis and their phytochemical screenings. | the in vitro antioxidant and antibacterial assays against clinically isolated streptococcus suis and staphylococcus intermedius of the extracts prepared by decoction and ethanolic reflux of different parts of chettaphangki (cladogynos orientalis zipp. ex span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (dpph) scavenging assay and disc diffusion method were conducted. quantitative analysis of total phenolic and total flavonoid contents in the extracts using spect ... | 2015 | 26347795 |
| live attenuated salmonella enterica serovar choleraesuis vaccine vector displaying regulated delayed attenuation and regulated delayed antigen synthesis to confer protection against streptococcus suis in mice. | salmonella enterica serotype choleraesuis (s. choleraesuis) and streptococcus suis (s. suis) are important swine pathogens. development of a safe and effective attenuated s. choleraesuis vaccine vector would open a new window to prevent and control pig diseases. to achieve this goal, the mannose and arabinose regulated delayed attenuated systems (rdas), δpmi and δpcrp::tt arac pbadcrp, were introduced into the wild type s. choleraesuis strain c78-3. we also introduced δrela::arac pbadlaci tt to ... | 2015 | 26238722 |
| prophage lysin ply30 protects mice from streptococcus suis and streptococcus equi subsp. zooepidemicus infections. | streptococcus suis and streptococcus equi subsp. zooepidemicus are capable of infecting humans and various animals, causing significant problems for the worldwide swine industry. as antibiotic resistance has increased, lysosomal enzymes encoded by phages have shown potential for use against pathogenic bacteria. in this study, a novel bacteriophage lysin, ply30, encoded by the s. suis prophage phi30c, was recombinantly expressed and purified. ply30 showed high bacteriolysis activity on s. suis an ... | 2015 | 26253669 |
| [streptococcal toxic shock-like syndrome due to streptococcus suis serotype 2 in a japanese pig farmer]. | streptococcus suis is a major swine pathogen. it has recently been recognized as an emerging zoonosis that causes mainly meningitis and sepsis in human. in particular, toxic shock-like syndrome (tsls) caused by this pathogen has a high mortality rate. however, misidentification of s. suis by conventional biochemical and commercial identification tests is not rare. the patient was a 71-year-old man who worked as a pig farmer who was admitted for fever, oliguria and subcutaneous hemorrhage. he was ... | 2015 | 26821523 |
| genetic diversity of streptococcus suis isolated from three pig farms of china obtained by acquiring antibiotic resistance genes. | acquiring antibiotic resistance genes may change an organism's genetic characteristics and the effect of antibiotics, resulting in a rapid transmission of microbial pathogens. the objectives of this experiment were to identify the features of streptococcus suis (s. suis) isolated from three pig farms in china which are geographically isolated. | 2015 | 25060787 |
| serotype- and virulence-associated gene profile of streptococcus suis isolates from pig carcasses in chiang mai province, northern thailand. | in this present study, the serotype of 40 streptococcus suis isolates from submaxillary glands of pig carcasses sold in wet markets in chiang mai province, northern thailand, was investigated. eleven serotypes, including types 2, 3, 4, 5, 7, 8, 9, 17, 21, 22 and 31, were found in the isolates by a multiplex pcr combined with serum agglutination. of the eleven serotypes present, type 3 was the most prevalent, while types 2, 4, 5 and 21 were of primary interest due to their human isolate serotype. ... | 2015 | 25367105 |
| novel variant serotype of streptococcus suis isolated from piglets with meningitis. | streptococcus suis is an emerging zoonotic pathogen causing severe infections in pigs and humans. in previous studies, 33 serotypes of s. suis have been identified using serum agglutination. here, we describe a novel s. suis strain, cz130302, isolated from an outbreak of acute piglet meningitis in eastern china. strong pathogenicity of meningitis caused by strain cz130302 was reproduced in the balb/c mouse model. the strain showed a high fatality rate (8/10), higher than those for known virulent ... | 2015 | 25416757 |
| reappraisal of the taxonomy of streptococcus suis serotypes 20, 22 and 26: streptococcus parasuis sp. nov. | in order to clarify the taxonomic position of serotypes 20, 22 and 26 of streptococcus suis, biochemical and molecular genetic studies were performed on isolates (sut-7, sut-286(t), sut-319, sut-328 and sut-380) reacted with specific antisera of serotypes 20, 22 or 26 from the saliva of healthy pigs as well as reference strains of serotypes 20, 22 and 26. comparative recn gene sequencing showed high genetic relatedness among our isolates, but marked differences from the type strain s. suis nctc ... | 2015 | 25385995 |
| construction, characterization and evaluation of the protective efficacy of the streptococcus suis double mutant strain δsspep/δsspspc as a live vaccine candidate in mice. | streptococcus suis serotype 2 (s. suis 2) causes sepsis and meningitis in piglets and humans, and results in one of the most serious bacterial diseases affecting the production of commercial pigs around the world. due to the failure of the current inactivated vaccine to protect against the disease, development of a new attenuated live vaccine against s. suis 2 by deleting essential virulence factors is urgently needed. we have previously reported the construction and characterization of an sspep ... | 2015 | 25220429 |
| identification and characterisation a surface-associated arginine peptidase in streptococcus suis serotype 2. | streptococcus suis is an important zoonotic pathogen worldwide and is responsible for disease in swine and humans. in the present study, we identified and characterised a surface-associated peptidase (abpb, amylase-binding protein b) in streptococcus suis serotype 2 (s. suis 2) that has high hydrolytic activity towards h-arg-pna, with maximum activity at ph 7.0. stimulation of raw 264.7 macrophages with purified recombinant abpb protein triggered the release of pro-inflammatory cytokines. an abp ... | 2015 | 25179447 |
| streptococcus suis sorption on agricultural soils: role of soil physico-chemical properties. | understanding pathogen sorption on natural soil particles is crucial to protect public health from soilborne and waterborne diseases. sorption of pathogen streptococcus suis on 10 agricultural soils was examined, and its correlations with soil physico-chemical properties were also elucidated. s. suis sorption isotherms conformed to the linear equation, with partition coefficients (ks) ranging from 12.7 ml g(-1) to 100.1 ml g(-1). bacteria were observed to sorb on the external surfaces of soil ag ... | 2015 | 24968305 |
| identification of novel laminin- and fibronectin-binding proteins by far-western blot: capturing the adhesins of streptococcus suis type 2. | bacterial cell wall (cw) and extracellular (ec) proteins are often involved in interactions with extracellular matrix (ecm) proteins such as laminin (ln) and fibronectin (fn), which play important roles in adhesion and invasion. in this study, an efficient method combining proteomic analysis and far-western blotting assays was developed to screen directly for bacterial surface proteins with ln- and fn-binding capacity. with this approach, fifteen potential ln-binding proteins and five potential ... | 2015 | 26636044 |
| targeting trem-1 signaling in the presence of antibiotics is effective against streptococcal toxic-shock-like syndrome (stsls) caused by streptococcus suis. | streptococcus suis (s.suis), a major swine pathogen, is also a severe threat to human health. infection with highly virulent strains of s. suis can cause human streptococcal toxic-shock-like syndrome (stsls), which is associated with high serum pro-inflammatory cytokine levels and a high mortality rate. our previous study indicated that highly virulent s. suis infection could activate the trem-1 signaling pathway, which promotes host clearance of s. suis during early infection. however, it remai ... | 2015 | 26618144 |
| hyaluronate lyase activity of streptococcus suis serotype 2 and modulatory effects of hyaluronic acid on the bacterium's virulence properties. | streptococcus suis serotype 2 is a major swine pathogen and zoonotic agent worldwide causing mainly meningitis and septicemia. hyaluronate lyases are enzymes that degrade hyaluronic acid, a major constituent of animal tissues, and have been reported as virulence factors in various bacterial species. since the hyaluronate lyase of s. suis has been considered ambiguously as a virulence factor, we screened 50 isolates from the three major clonal complexes found in north america (sequence type [st] ... | 2015 | 26611338 |
| whole genome investigation of a divergent clade of the pathogen streptococcus suis. | streptococcus suis is a major porcine and zoonotic pathogen responsible for significant economic losses in the pig industry and an increasing number of human cases. multiple isolates of s. suis show marked genomic diversity. here, we report the analysis of whole genome sequences of nine pig isolates that caused disease typical of s. suis and had phenotypic characteristics of s. suis, but their genomes were divergent from those of many other s. suis isolates. comparison of protein sequences predi ... | 2015 | 26583006 |
| draft genome sequences of nine streptococcus suis strains isolated in the united states. | streptococcus suis is a swine pathogen responsible for economic losses to the pig industry worldwide. additionally, it is a zoonotic agent that can cause severe infections in those in close contact with infected pigs and/or who consume uncooked or undercooked pork products. here, we report nine draft genome sequences of s. suis. | 2015 | 26543125 |
| two novel regulators of n-acetyl-galactosamine utilization pathway and distinct roles in bacterial infections. | bacterial pathogens can exploit metabolic pathways to facilitate their successful infection cycles, but little is known about roles of d-galactosamine (galn)/n-acetyl-d-galactosamine (galnac) catabolism pathway in bacterial pathogenesis. here, we report the genomic reconstruction of galn/galnac utilization pathway in streptococci and the diversified aga regulons. we delineated two new paralogous agar regulators for the galn/galnac catabolism pathway. the electrophoretic mobility shift assays exp ... | 2015 | 26540018 |
| a novel endolysin disrupts streptococcus suis with high efficiency. | streptococcus suis serotype 2 (s. suis 2) is a zoonotic pathogen that exhibits high-level resistance and multi-drug resistance to classic antibiotics and causes serious human casualties and heavy economic losses in the swine industry worldwide. therefore, alternative therapies or novel antibacterial agents need to be developed to combat this pathogen. a novel endolysin derived from the s. suis temperate phage phi7917, termed ly7917, was identified, which had broad lytic activity against s. suis ... | 2015 | 26534896 |
| streptococcus suis vaccines: candidate antigens and progress. | streptococcus suis is a major swine pathogen and an emerging zoonotic agent of human meningitis and streptococcal toxic shock-like syndrome. s. suis is a well-encapsulated pathogen and multiple serotypes have been described based on the capsular polysaccharide antigenic diversity. in addition, high genotypic, phenotypic and geographic variability exits among strains within the same serotype. besides, s. suis uses an arsenal of virulence factors to evade the host immune system. together, these ch ... | 2015 | 26468755 |
| emodin affects biofilm formation and expression of virulence factors in streptococcus suis atcc700794. | streptococcus suis (s. suis) is a swine pathogen and also a zoonotic agent. in this study, the effects of subinhibitory concentrations (sub-mics) of emodin on biofilm formation by s. suis atcc700794 were evaluated. as quantified by crystal violet staining, biofilm formation by s. suis atcc700794 was dose-dependently decreased after growth with 1/2 mic, 1/4 mic, or 1/8 mic of emodin. by scanning electron microscopy, the structural architecture of the s. suis atcc700794 biofilms was examined follo ... | 2015 | 26446827 |
| interaction of fibrinogen and muramidase-released protein promotes the development of streptococcus suis meningitis. | muramidase-released protein (mrp) is as an important virulence marker of streptococcus suis (s. suis) serotype 2. our previous works have shown that mrp can bind human fibrinogen (hfg); however, the function of this interaction in s. suis meningitis is not known. in this study, we found that the deletion of mrp significantly impairs the hfg-mediated adherence and traversal ability of s. suis across human cerebral microvascular endothelial cells (hcmec/d3). measurement of the permeability to luci ... | 2015 | 26441928 |
| third case of streptococcus suis infection in greece. | streptococcus suis is a facultative anaerobic, gram-positive coccus that can cause severe disease to both pigs and humans. its zoonotic potential was first recognized in 1968 when the first human case of meningitis was reported in denmark. since then, over 1600 human cases have been reported worldwide, the vast majority of which originated in southeast asia, and, thus, s. suis has been fairly characterized as an emerging pathogen. infection in humans presents most commonly as bacteremia and/or m ... | 2015 | 26435860 |
| antibacterial effect of porcine ptx3 against streptococcus suis type 2 infection. | pentraxin 3 (ptx3), a soluble pattern recognition receptor, plays an important role in innate immunity and has been implicated to be a candidate resistance gene against streptococcus suis 2 infection. to discover the antibacterial effect of porcine ptx3 against s. suis 2, the 42-kda ptx3 protein was expressed by chinese hamster ovary cells (cho), and an additional eukaryotic expression vector pvax-ptx3 was constructed. the expressed porcine ptx3 mediated a range of antibacterial activities inclu ... | 2015 | 26427882 |
| clonal distribution of streptococcus suis isolated from diseased pigs in the central region of chile. | the characteristics of 29 chilean field strains of streptococcus suis recovered between 2007 and 2011 from pigs with clinical signs at different farms were studied. serotyping with use of the coagglutination test revealed that all but 1 strain belonged to serotype 6; the remaining strain was serotype 22. all the serotype-6 strains were suilysin (hemolysin)-negative; in addition, they were found to be genotypically homogeneous by enterobacterial repetitive intergenic consensus sequence-based poly ... | 2015 | 26424917 |
| streptococcus suis in employees and the environment of swine slaughterhouses in são paulo, brazil: occurrence, risk factors, serotype distribution, and antimicrobial susceptibility. | streptococcus suis is an important pathogen in the swine industry. this article is the first to report the occurrence, risk factors, serotype distribution, and antimicrobial susceptibility of s. suis recovered from employees and environmental samples of swine slaughterhouses in brazil. tonsillar swabs from all 139 pig-slaughtering employees and 261 environmental swabs were collected for detection of s. suis and serotyping by monoplex and multiplex polymerase chain reaction, respectively. antimic ... | 2015 | 26424907 |
| characterization of five zoonotic streptococcus suis strains from germany, including one isolate from a recent fatal case of streptococcal toxic shock-like syndrome in a hunter. | a streptococcus suis isolate from a german hunter with streptococcal toxic shock-like syndrome (stsls) and four additional zoonotic isolates were genotyped as mrp(+) epf* (variant 1890) sly(+) cps2(+). all five zoonotic german strains were characterized by high multiplication in human blood samples ex vivo, but induction of only low levels of proinflammatory cytokines compared to a chinese stsls strain. | 2015 | 26424844 |
| first human case report of sepsis due to infection with streptococcus suis serotype 31 in thailand. | streptococcus suis is a zoonotic pathogen that causes invasive infections in humans and pigs. it has been reported that s. suis infection in humans is mostly caused by serotype 2. however, human cases caused by other serotypes have rarely been reported. this is the first report of a human case of infection with s. suis serotype 31 in thailand. | 2015 | 26420029 |
| complex population structure and virulence differences among serotype 2 streptococcus suis strains belonging to sequence type 28. | streptococcus suis is a major swine pathogen and a zoonotic agent. serotype 2 strains are the most frequently associated with disease. however, not all serotype 2 lineages are considered virulent. indeed, sequence type (st) 28 serotype 2 s. suis strains have been described as a homogeneous group of low virulence. however, st28 strains are often isolated from diseased swine in some countries, and at least four human st28 cases have been reported. here, we used whole-genome sequencing and animal i ... | 2015 | 26375680 |
| assessing the metabolic diversity of streptococcus from a protein domain point of view. | understanding the diversity and robustness of the metabolism of bacteria is fundamental for understanding how bacteria evolve and adapt to different environments. in this study, we characterised 121 streptococcus strains and studied metabolic diversity from a protein domain perspective. metabolic pathways were described in terms of the promiscuity of domains participating in metabolic pathways that were inferred to be functional. promiscuity was defined by adapting existing measures based on dom ... | 2015 | 26366735 |
| expression, purification, crystallization and structure determination of the n terminal domain of fhb, a factor h binding protein from streptococcus suis. | fhb is a surface virulence protein from streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor h to inactivate c3b deposited on bacterial surface in blood. here we successfully expressed and purified the n terminal domain of fhb (n-fhb) and obtained crystals of the n-fhb by sitting-drop vapor diffusion method with a resolution of 1.50 å. the crystals belong to space group c2 with unit cell parameters a = 127.1 å, b = 77.3 å, ... | 2015 | 26365348 |
| emergence of streptococcus suis serotype 9 infection in humans. | 2015 | 26362754 | |
| assessment of maldi-tof ms as alternative tool for streptococcus suis identification. | the accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) for identifying streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a pcr-based identification assay as the gold standard. in addition, maldi-tof ms was compared with the commercial multi-tests rapid id 32 strep system. from the 129 s. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score val ... | 2015 | 26347858 |
| virulence genotyping and population analysis of streptococcus suis serotype 2 isolates from china. | streptococcus suis is one of the most important swine pathogens worldwide. in this study, a total of 22 virulence-related genes in 101 strains of s. suis serotype 2 (ss2) were detected by pcr, namely, mrp, epf, sly, fbps, rgg, ofs, srta, pgda, gapdh, iga, endod, ciarh, salkr, mann, purd, rgg, dppiv, neub, dlta, smu_61-like, spym3_0908 (permease) and the sspa gene. the distribution of virulence-related genes among isolates was visualized using bionumerics software to study similarities among the ... | 2015 | 26303637 |
| catabolite control protein a has an important role in the metabolic regulation of streptococcus suis type 2 according to itraq-based quantitative proteomic analysis. | the catabolite control protein a (ccpa) regulates the carbon metabolism in streptococcus suis type 2 and has pleiotropic regulatory functions in bacterial virulence and transcription. the present study systematically investigated ccpa activity in streptococcus suis type 2 using isobaric tag for relative and absolute quantification (itraq) liquid chromatography‑tandem mass spectrometry‑based proteomics. gene ontology and kyoto encyclopedia of genes and genomes analyses demonstrated that ccpa is a ... | 2015 | 26299628 |
| [correlation between type iv secretion system component vird4 and virulence for streptococcus suis 2]. | in order to study the role of ss2 type iv secretion system vird4 in evasion of the host innate immune killing, we constructed a knockout mutant avird4. then we studied its biological activity and virulence. | 2015 | 26259489 |
| msmk, an atpase, contributes to utilization of multiple carbohydrates and host colonization of streptococcus suis. | acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. different bacteria can uptake different lines of carbohydrates via abc transporters, in which atpase subunits energize the transport though atp hydrolysis. some abc transporters possess their own atpases, while some share a common atpase. here we identified msmk, an atpase from streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. genet ... | 2015 | 26222651 |
| [lytic phages and prophages of streptococcus suis--a review]. | streptococcus suis (s. suis) is an important zoonosis and pathogen that can carry prophages. in this review, we focus on the recent advances in our understanding of lytic phage and lysogenic phage of s. suis, including the morphology of s. suis lytic phage, the functions of lysin and terminase large subunit encoded by s. suis lytic phage, comparative genomics of s. suis prophages, lysogenic. conversion between s. suis lytic phage and prophage. furthermore, prospective evolution of interactions b ... | 2015 | 26211312 |
| simultaneous detection of 33 streptococcus suis serotypes using the luminex xtag® assay™. | we report the development and evaluation of a serotype-specific xtag luminex assay (ssa) that allows detection of the 33 serotypes of streptococcus suis (s. suis). this assay is based on wzy gene targets directly involved in the cps biosynthesis and can be completed 40 min post-pcr amplification. the assay correctly and specifically identified the serotype of all 209 isolates tested, in comparison with two serotyping multiplex pcr methods previously developed. the sensitivity was higher than tha ... | 2015 | 26210040 |
| investigation into the role of catabolite control protein a in the metabolic regulation of streptococcus suis serotype 2 using gene expression profile analysis. | catabolite control protein a (ccpa) serves a key function in the catabolism of streptococcus suis serotype 2 (s. suis 2) by affecting the biological function and metabolic regulatory mechanisms of this bacterium. the aim of the present study was to identify variations in ccpa expression in s. suis 2 using gene expression profile analysis. using sequencing and functional analysis, ccpa was demonstrated to play a regulatory role in the expression and regulation of virulence genes, carbon metabolis ... | 2015 | 26170923 |
| toll-like receptor 4 confers inflammatory response to suilysin. | streptococcus suis serotype 2 (ss2) is an emerging human pathogen worldwide. a large outbreak occurred in the summer of 2005 in china. serum samples from this outbreak revealed that levels of the main proinflammatory cytokines were significantly higher in patients with streptococcal toxic-shock-like syndrome (stsls) than in patients with meningitis only. however, the mechanism underlying the cytokine storm in stsls caused by ss2 remained unclear. in this study, we found that suilysin (sly) is th ... | 2015 | 26167160 |
| streptococcus suis meningitis in the netherlands. | 2015 | 26165610 | |
| the type ii histidine triad protein htpsc is a novel adhesion with the involvement of streptococcus suis virulence. | streptococcal histidine triad proteins htps are widely distributed within the streptococcus genus. based on the phylogenetic relationship and domain composition, htps are classified into type i and type ii subfamilies. previous studies revealed that several pathogenic streptococci contain more than one htp gene. we found that the highly virulent strain of streptococcus suis 2 (s. suis 2), 05zyh33 encodes 3 htps, designated htpsa (previously described as htps), htpsb, and htpsc. among them, htpsc ... | 2015 | 26151575 |
| molecular diagnostics and its-based phylogenic analysis of streptococcus suis serotype 2 in central vietnam. | streptococcus suis (s. suis) serotype 2 has recently become the most prevalent cause of meningitis in adults in many areas of vietnam. this study provides data on s. suis molecular diagnosis in central vietnam using a real-time polymerase chain reaction (pcr) assay targeting the s. suis serotype 2 cps2j gene. additionally, 16s-23s rdna intragenic spacer (its)-based phylogenic analysis of strains isolated from cerebrospinal fluid (csf) in thua thien hue province, vietnam, is presented and discuss ... | 2015 | 26142672 |
| the chromosomal sezat toxin-antitoxin system promotes the maintenance of the sspi-1 pathogenicity island in epidemic streptococcus suis. | streptococcus suis has emerged as a causative agent of human meningitis and streptococcal toxic shock syndrome over the last years. the high pathogenicity of s. suis may be due in part to a laterally acquired pathogenicity island (renamed sspi-1), which can spontaneously excise and transfer to recipients. cells harboring excised sspi-1 can potentially lose this island if cell division occurs prior to its reintegration; however, attempts to cure sspi-1 from the host cells have been unsuccessful. ... | 2015 | 26138696 |
| erratum: genomic signatures of human and animal disease in the zoonotic pathogen streptococcus suis. | 2015 | 25966020 | |
| the immunoglobulin m-degrading enzyme of streptococcus suis, idessuis, is involved in complement evasion. | streptococcus (s.) suis is one of the most important pathogens in pigs causing meningitis, arthritis, endocarditis and serositis. furthermore, it is also an emerging zoonotic agent. in our previous work we identified a highly specific igm protease in s. suis, designated ide(ssuis) . the objective of this study was to characterize the function of ide(ssuis) in the host-pathogen interaction. edman-sequencing revealed that ide(ssuis) cleaves the heavy chain of the igm molecule between constant doma ... | 2015 | 25928761 |
| [epidemiological investigation of the first human case infected with streptococcus suis in shandong]. | 2015 | 25924244 | |
| molecular basis of resistance to selected antimicrobial agents in the emerging zoonotic pathogen streptococcus suis. | characterization of 227 streptococcus suis strains isolated from pigs during 2010 to 2013 showed high levels of resistance to clindamycin (95.6%), tilmicosin (94.7%), tylosin (93.8%), oxytetracycline (89.4%), chlortetracycline (86.8%), tiamulin (72.7%), neomycin (70.0%), enrofloxacin (56.4%), penicillin (56.4%), ceftiofur (55.9%), and gentamicin (55.1%). resistance to tetracyclines, macrolides, aminoglycosides, and fluoroquinolone was attributed to the tet gene, erm(b), erm(c), mph(c), and mef(a ... | 2015 | 25903569 |
| candidate proteomic biomarkers for three genogroups of the swine pathogen streptococcus suis serotype 2. | streptococcus suis, more specifically serotype 2, is a major swine pathogen and an emerging zoonotic agent that causes severe infections such as meningitis, endocarditis, and septicemia. in this study, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (seldi) was used to investigate the protein expression profiles of 45 strains of s. suis serotype 2 that had previously been clustered by multilocus sequence typing (mlst) into three sequence types (st1, st25, and st28) ... | 2015 | 25886383 |
| multilocus sequence typing of australian streptococcus suis type 2 by maldi-tof mass spectrometry analysis of pcr amplicons. | streptococcus suis serotype 2 is a ubiquitous pathogen of swine and is known to cause severe disease in humans. multilocus sequence typing (mlst) is ideal for characterising this organism because it permits isolates to be compared on a national and international scale. a novel approach to mlst using matrix-assisted laser desorption ionisation-time of flight mass spectrometry (ms-mlst) provides a more rapid alternative to dideoxy sequencing. this study used ms-mlst to define the multilocus sequen ... | 2015 | 25862280 |
| eight novel capsular polysaccharide synthesis gene loci identified in nontypeable streptococcus suis isolates. | streptococcus suis is an important pathogen of pigs and may cause serious disease in humans. serotyping is one of the important diagnostic tools and is used for the epidemiological study of s. suis. nontypeable s. suis strains have been reported in many studies; however, the capsular polysaccharide (cps) synthesis cps loci of nontypeable strains have not been analyzed. in this study, we investigated the genetic characteristics of cps loci in 78 nontypeable strains isolated from healthy pigs. eig ... | 2015 | 25862221 |
| role of the capsular polysaccharide as a virulence factor for streptococcus suis serotype 14. | streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia. although serotype 2 is the most virulent type, serotype 14 is emerging, and understanding of its pathogenesis is limited. to study the role of the capsular polysaccharide (cps) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14b, a highly conserved regulatory gene, or neu14c, a gene coding for uridine diphospho-n-acetylglucosamine 2-epimerase, which ... | 2015 | 25852230 |
| the immunoglobulin m-degrading enzyme of streptococcus suis, idessuis, is a highly protective antigen against serotype 2. | streptococcus suis (s. suis) is a major porcine pathogen causing meningitis, arthritis and several other pathologies. recently, we identified a highly specific immunoglobulin m degrading enzyme of s. suis, designated idessuis, which is expressed by various serotypes. the objective of this work was to access the immunogenicity and protective efficacy of a recombinant vaccine including idessuis. vaccination with ridessuis elicited antibodies efficiently neutralizing the igm protease activity. impo ... | 2015 | 25825330 |
| genomic signatures of human and animal disease in the zoonotic pathogen streptococcus suis. | streptococcus suis causes disease in pigs worldwide and is increasingly implicated in zoonotic disease in east and south-east asia. to understand the genetic basis of disease in s. suis, we study the genomes of 375 isolates with detailed clinical phenotypes from pigs and humans from the united kingdom and vietnam. here, we show that isolates associated with disease contain substantially fewer genes than non-clinical isolates, but are more likely to encode virulence factors. human disease isolate ... | 2015 | 25824154 |
| evaluation of the protective efficacy of four novel identified membrane associated proteins of streptococcus suis serotype 2. | streptococcus suis serotype 2 (s. suis 2) is an important zoonotic pathogen that can also cause epidemics of life-threatening infections in humans. surface proteins of pathogens play a critical role in the interaction with host system or environment, as they take part in processes like virulence, cytotoxicity, adhesion, signaling or transport, etc. thus, surface proteins identified by the screening of immunoproteomic techniques are promising vaccine candidates or diagnostic markers. in this stud ... | 2015 | 25820064 |
| a novel pro-inflammatory protein of streptococcus suis 2 induces the toll-like receptor 2-dependent expression of pro-inflammatory cytokines in raw 264.7 macrophages via activation of erk1/2 pathway. | streptococcus suis 2 is an important swine pathogen and an emergent zoonotic pathogen. excessive inflammation caused by s. suis is responsible for the high levels of early mortality observed in septic shock-like syndrome cases. however, the mechanisms through which s. suis 2 (ss2) causes excessive inflammation remain unclear. thus, this study aimed to identify novel pro-inflammatory mediators that play important roles in the development of therapies against ss2 infection. in this study, the nove ... | 2015 | 25806027 |
| proteomics identification of novel fibrinogen-binding proteins of streptococcus suis contributing to antiphagocytosis. | streptococcus suis serotype 2 (ss2) induced sepsis and meningitis are often accompanied by bacteremia. however, the mechanism whereby it helps s. suis to evade pmn-mediated phagocytosis remain unclear. because of the central roles of bacteria-human fibrinogen (hfg) interaction in innate immunity, here, a proteomics based far-western blotting (pbfwb) was developed to identify the fibrinogen-binding surface proteins of s. suis (ssfbps) on a large-scale. and then thirteen potential ssfbps were iden ... | 2015 | 25789245 |
| genetic diversity and virulence of novel sequence types of streptococcus suis from diseased and healthy pigs in china. | streptococcus suis is a serious threat to swine industry and public health. in this work, a total of 62 s. suis isolates recovered from infected and healthy pigs from four provinces in northern china were classified by multilocus sequence typing into nine sequence types (sts), including six novel ones, namely, st417, st418, st419, st420, st421, and st422. the majority (64.5%) of these 62 isolates belong to serotype 2; all of these serotype 2 isolates can be assigned into st1 or st28 clonal compl ... | 2015 | 25784908 |
| genome-wide identification of allele-specific expression in response to streptococcus suis 2 infection in two differentially susceptible pig breeds. | although allele expression imbalance has been recognized in many species, and strongly linked to diseases, no whole transcriptome allele imbalance has been detected in pigs during pathogen infections. the pathogen streptococcus suis 2 (ss2) causes serious zoonotic disease. different pig breeds show differential susceptibility/resistance to pathogen infection, but the biological insight is little known. here we analyzed allele-specific expression (ase) using the spleen transcriptome of four pigs ... | 2015 | 25737137 |
| superoxide dismutase of streptococcus suis serotype 2 plays a role in anti-autophagic response by scavenging reactive oxygen species in infected macrophages. | streptococcus suis serotype 2 (ss2) causes septic shock and meningitis. however, its pathogenesis is still not well-understood. we have recently shown that superoxide dismutase soda of ss2 is a virulence factor probably by increasing resistance to oxidative stresses. reactive oxygen species (ros) are products of the respiratory burst of phagocytic cells and have been shown to activate autophagy. we wanted to know if and how ss2 explores its soda to interfere with cell autophagic responses. a sod ... | 2015 | 25726301 |
| streptococcus suis serotype 2 strains can induce the formation of neutrophil extracellular traps and evade trapping. | streptococcus suis (s. suis) ranks among the five most important porcine pathogens worldwide and occasionally threatens human health, especially in people that come into close contact with pigs or pork products. streptococcus suis serotype 2 (ss2) is considered to be the most pathogenic and prevalent capsular type. as a first line of immune defense against ss2 infection, neutrophils can eliminate the invader not only by phagocytosis but also by neutrophil extracellular traps (nets)-mediated kill ... | 2015 | 25673283 |
| identification of a novel dnase of streptococcus suis (endasuis) important for neutrophil extracellular trap degradation during exponential growth. | the porcine and human pathogen streptococcus suis induces and degrades neutrophil extracellular traps (nets) in vitro. in this study, we investigated the working hypothesis that net degradation is mediated not only by the known secreted s. suis nuclease a (ssna) but also by a so-far undescribed putative endonuclease a of s. suis (designated endasuis) homologous to the pneumococcal endonuclease a (enda). comparative analysis was conducted to identify differences in localization, expression and fu ... | 2015 | 25667008 |
| streptococcus suis infection in hospitalized patients, nakhon phanom province, thailand. | in nakhon phanom, thailand, we identified 38 hospitalized patients with streptococcus suis infection during 2006-2012. deafness developed in 12 patients; none died. thirty-five reported recent exposure to pigs/pork. annual incidence was 0.1-2.2 cases/100,000 population (0.2-3.2 in persons ≥20 years of age). clinicians should consider s. suis infection in areas where pig exposure is common. | 2015 | 25625540 |
| quantitative proteomic analysis of sub-mic erythromycin inhibiting biofilm formation of s. suis in vitro. | streptococcus suis (s. suis) is a swine pathogen and also a zoonotic agent. biofilms of s. suis may cause persistent infections by the host immune system and antibiotics. sub-minimal inhibitory concentration (sub-mic) of erythromycin can inhibit biofilm formation in bacteria. here, we performed comparative proteomic analyses of cells at two different conditions: sub-mic erythromycin treated and nontreated cells. using itraq strategy, we found some novel proteins that involved in biofilm formatio ... | 2015 | 25579403 |
| characterization of the pivotal carbon metabolism of streptococcus suis serotype 2 under ex vivo and chemically defined in vitro conditions by isotopologue profiling. | streptococcus suis is a neglected zoonotic pathogen that has to adapt to the nutritional requirements in the different host niches encountered during infection and establishment of invasive diseases. to dissect the central metabolic activity of s. suis under different conditions of nutrient availability, we performed labeling experiments starting from [(13)c]glucose specimens and analyzed the resulting isotopologue patterns in amino acids of s. suis grown under in vitro and ex vivo conditions. i ... | 2015 | 25575595 |
| dnaj of streptococcus suis type 2 contributes to cell adhesion and thermotolerance. | to examine if the molecular chaperone dnak operon proteins of streptococcus suis type 2 (ss2) are involved in adhesion to host cells, the abundance values of these proteins from the surface of two ss2 strains of different adhesion capability were compared. their roles in growth and adhesion to human laryngeal epithelial cell line hep-2 cells were investigated on ss2 strain ha9801 and its mutants with dnak operon genes partially knocked-out (pko mutant) under heat stress. the major difference was ... | 2015 | 25537722 |
| prioritization of zoonotic diseases of public health significance in vietnam. | prioritization of zoonotic diseases is critical as it facilitates optimization of resources, greater understanding of zoonotic diseases and implementation of policies promoting multisectoral collaboration. this study aimed to establish strategic priorities for zoonotic diseases in vietnam taking a key stakeholder approach. | 2015 | 26719937 |
| antimicrobial resistance profile and genotypic characteristics of streptococcus suis capsular type 2 isolated from clinical carrier sows and diseased pigs in china. | streptococcus suis serotype 2 is an important zoonotic pathogen. antimicrobial resistance phenotypes and genotypic characterizations of s. suis 2 from carrier sows and diseased pigs remain largely unknown. in this study, 96 swine s. suis type 2, 62 from healthy sows and 34 from diseased pigs, were analyzed. high frequency of tetracycline resistance was observed, followed by sulfonamides. the lowest resistance of s. suis 2 for β-lactams supports their use as the primary antibiotics to treat the i ... | 2015 | 26064892 |
| fatal case of streptococcus suis infection in a young wild boar (sus scrofa) from southwestern spain. | streptococcus suis is a recognized pathogen that may cause important diseases in pigs and humans. this microorganism has been repeatedly isolated from wild boar (sus scrofa). however, its health implications for this wild species are still unknown. this article reports a detailed description of a fatal case of septicemia by s. suis affecting a young wild boar. the affected animal, about 15 days old, was found near death and exhibiting neurologic signs at a wild boar estate in southwestern spain. ... | 2015 | 26056897 |
| development of loop-mediated isothermal amplification to detect streptococcus suis and its application to retail pork meat in japan. | we here developed a novel loop-mediated isothermal amplification (lamp) method to detect streptococcus suis in raw pork meat. this method, designated lampss, targeted the recombination/repair protein (recn) gene of s. suis and detected all serotypes of s. suis, except those taxonomically removed from authentic s. suis, i.e., serotypes 20, 22, 26, 32, 33, and 34. the specificity of lampss was confirmed and its detection limit was 5.4cfu/reaction. among the 966 raw pork meat samples examined, incl ... | 2015 | 26043307 |
| host-pathogen interaction at the intestinal mucosa correlates with zoonotic potential of streptococcus suis. | streptococcus suis has emerged as an important cause of bacterial meningitis in adults. the ingestion of undercooked pork is a risk factor for human s. suis serotype 2 (ss2) infection. here we provide experimental evidence indicating that the gastrointestinal tract is an entry site of ss2 infection. | 2015 | 25525050 |
| molecular typing of streptococcus suis isolates from iberian pigs: a comparison with isolates from common intensively-reared commercial pig breeds. | the iberian pig (ip) is a traditional spanish breed variety of the domestic pig (sus scrofa domesticus) with high economic importance because of the value of the dry-cured products in national and international markets. the genetic characteristics of tonsillar and clinical streptococcus suis isolates from the ip maintained under extensive or intensive management conditions were investigated. s. suis isolates from ip pigs were compared with s. suis isolates from intensively-farmed pigs of common ... | 2014 | 25458888 |
| stepwise visualization of membrane pore formation by suilysin, a bacterial cholesterol-dependent cytolysin. | membrane attack complex/perforin/cholesterol-dependent cytolysin (macpf/cdc) proteins constitute a major superfamily of pore-forming proteins that act as bacterial virulence factors and effectors in immune defence. upon binding to the membrane, they convert from the soluble monomeric form to oligomeric, membrane-inserted pores. using real-time atomic force microscopy (afm), electron microscopy (em), and atomic structure fitting, we have mapped the structure and assembly pathways of a bacterial c ... | 2014 | 25457051 |
| a new recombinant ssna protein combined with aluminum hydroxide protects mouse against streptococcus suis. | an experimental challenge in a mouse model was used to select the most effective adjuvant in a vaccine formulation with the surface-anchored dna-nuclease (ssna). we used a protocol based on clinical, histopathological, bacterial kinetics and immune response against s. suis serotype 2 in infected animals. the three adjuvants used, aluminum hydroxide (aloh), incomplete freund's adjuvant (fia), oil-in-water adjuvant (ow) showed a protective effect against death by s. suis serotype 2 in this mouse m ... | 2014 | 25454868 |
| identification of major streptococcus suis serotypes 2, 7, 8 and 9 isolated from pigs and humans in upper northeastern thailand. | streptococcus suis serotype 2 infections occur in many provinces of north-eastern thailand, knowledge concerning the prevalence of the common s. suis serotypes (1, 1/2, 2, 5, 7, 8, 9, 14 and 16) among healthy and diseased pigs in upper northeastern thailand remains limited. this study investigated s. suis isolates from pigs (healthy and diseased) and also from humans using 11 conventional biochemical tests, 16s rdna pcr and sequence analysis and multiplex pcr genotyping of porcine cps and gdh. t ... | 2014 | 25417521 |
| streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing. | streptococcus suis is an important pathogen causing economic problems in the pig industry. moreover, it is a zoonotic agent causing severe infections to people in close contact with infected pigs or pork-derived products. although considered sporadic in the past, human s. suis infections have been reported during the last 45 years, with two large outbreaks recorded in china. in fact, the number of reported human cases has significantly increased in recent years. in this review, we present the wo ... | 2014 | 26038745 |
| in vitro transcriptome analysis of two chinese isolates of streptococcus suis serotype 2. | the streptococcus suis serotype 2 (s. suis 2) isolates 05zyh33 and 98hah33 have caused severe human infections in china. using a strand-specific rna-seq analysis, we compared the in vitro transcriptomes of these two chinese isolates with that of a reference strain (p1/7). in the 89k genomic island that is specific to these chinese isolates, a toxin-antitoxin system showed relatively high levels of transcription among the s. suis. the known virulence factors with high transcriptional activity in ... | 2014 | 25526982 |
| control of competence for dna transformation in streptococcus suis by genetically transferable pherotypes. | here we show that s. suis, a major bacterial pathogen of pigs and emerging pathogen in humans responds to a peptide pheromone by developing competence for dna transformation. this species does not fall within any of the phylogenetic clusters of streptococci previously shown to regulate competence via peptide pheromones suggesting that more species of streptococci may be naturally competent. induction of competence was dependent on comx, a sigma factor that controls the streptococcal late compete ... | 2014 | 24968201 |
| latest developments on streptococcus suis: an emerging zoonotic pathogen: part 2. | first international workshop on streptococcus suis, beijing, china, 12-13 august 2013. this second and final chapter of the report on the first international workshop on streptococcus suis follows on from part 1, published in the april 2014, volume 9, issue 4 of future microbiology. s. suis is a swine pathogen and a zoonotic agent afflicting people in close contact with infected pigs or pork meat. although sporadic cases of human infections had been reported worldwide, deadly s. suis outbreaks e ... | 2014 | 24957086 |
| gluconate 5-dehydrogenase (ga5dh) participates in streptococcus suis cell division. | bacterial cell division is strictly regulated in the formation of equal daughter cells. this process is governed by a series of spatial and temporal regulators, and several new factors of interest to the field have recently been identified. here, we report the requirement of gluconate 5-dehydrogenase (ga5dh) in cell division of the zoonotic pathogen streptococcus suis. ga5dh catalyzes the reversible reduction of 5-ketogluconate to d-gluconate and was localized to the site of cell division. the d ... | 2014 | 24916441 |
| characterization of multi-drug tolerant persister cells in streptococcus suis. | persister cells constitute a subpopulation of dormant cells within a microbial population which are genetically identical but phenotypically different to regular cells. notably, persister cells show an elevated tolerance to antimicrobial agents. thus, they are considered to represent a microbial 'bet-hedging' strategy and are of particular importance in pathogenic bacteria. | 2014 | 24885389 |
| [clinical situation, diagnosis and prevention of a streptococcus suis serotype 7 problem on a farm]. | in an austrian piglet producing farm with 1500 sows a high incidence of meningitis, arthritis and sudden death was recorded in five to eight week old piglets. overall losses were 1.8%. streptococcus (s.) suis serotype 7 was detected with an intermediate to high specific bacterial load in all samples taken from brains and joints of 17 untreated piglets with typical clinical signs. all isolates showed an identical spectrum of virulence-associated genes (mrp+, epf-, ofs-, sly-) and expressed a rela ... | 2014 | 24881269 |
| streptococcus suis infection and malignancy in man, spain. | 2014 | 24856779 | |
| overexpression of an abc transporter and mutations of gyra, gyrb, and parc in contributing to high-level ciprofloxacin resistance in streptococcus suis type 2. | streptococcus suis is a pathogen of zoonotic diseases. moreover, the emergence of fluoro-quinolones (fqs) resistance in this pathogen has severe consequences for pigs and human health. in this study, the molecular mechanism of fqs resistance in s. suis type 2 (ss2) sensitive strains isolated from pigs was assessed after in vitro induction of resistance against the most frequently used fqs: ciprofloxacin, norfloxacin, and enrofloxacin. proteome analysis, sequencing and real-time rt-pcr results st ... | 2014 | 24815385 |
| effect of tran on virulence through regulating metabolism and stress tolerance of streptococcus suis serotype 2. | streptococcus suis (ss) is an important zoonotic pathogen causing a variety of life-threatening infections in pigs and humans. tran, a novel transcriptional regulator which was identified to be an infection-related factor in s. suis serotype 2 using suppression subtractive hybridization (ssh), has been reported by our group. in this study, a tran deletion mutant was constructed to compare with the wild-type zy05719 in some biological characteristics. it is suggested that longer chains and relati ... | 2014 | 24813303 |
| latest developments on streptococcus suis: an emerging zoonotic pathogen: part 1. | first international workshop on streptococcus suis, beijing, china, 12-13 august 2013 the first international workshop on streptococcus suis, which is an important swine pathogen and emerging zoonotic agent, took place in beijing, jointly organized by the faculty of veterinary medicine, university of montreal, canada and the national institute for communicable disease control and prevention, china cdc. the aim of the meeting was to gather together, for the first time, more than 80 researchers wo ... | 2014 | 24810343 |
| the streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid. | streptococcus suis (ss) is an important pathogen of pigs, and it is also recognized as a zoonotic agent for humans. ss infection may result in septicemia or meningitis in the host. however, little is known about genes that contribute to the virulence process and survival within host blood or cerebrospinal fluid (csf). small rnas (srna) have emerged as key regulators of virulence in several bacteria, but they have not been investigated in ss. here, using a differential rna-sequencing approach and ... | 2014 | 24759092 |
| correlation between pfge groups and mrp/epf/sly genotypes of human streptococcus suis serotype 2 in northern thailand. | streptococcus suis infection is a severe zoonotic disease commonly found in northern thailand where people often consume raw pork and/or pig's blood. the most frequent clinical presentations are meningitis, sepsis, and endocarditis with higher rate of mortality and hearing loss sequelae. to clarify the correlation between pulsed-field gel electrophoresis (pfge) groups and mrp/epf/sly genotypes of s. suis serotype 2, 62 patient and 4 healthy pig isolates from northern thailand were studied. by pf ... | 2014 | 24734186 |