Publications

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activation of bacteroides fragilis toxin by a novel bacterial protease contributes to anaerobic sepsis in mice.bacteroides fragilis is the leading cause of anaerobic bacteremia and sepsis. enterotoxigenic strains that produce b. fragilis toxin (bft, fragilysin) contribute to colitis and intestinal malignancy, yet are also isolated in bloodstream infection. it is not known whether these strains harbor unique genetic determinants that confer virulence in extra-intestinal disease. we demonstrate that bft contributes to sepsis in mice, and we identify a b. fragilis protease called fragipain (fpn) that is req ...201627089515
a comparative evaluation of antibacterial effectiveness of sodium hypochlorite, curcuma longa, and camellia sinensis as irrigating solutions on isolated anaerobic bacteria from infected primary teeth.in endodontics, most of the commercial intra-canal medicaments have cytotoxic reactions and because of their inability to eliminate bacteria from dentinal tubules, recent medicine has turned its attention to the usage of biologic medication prepared from natural plants. the literature to testify the efficacy of natural alternatives in primary teeth is meagre and its effects as irrigating solutions need to be evaluated.201627080968
dendritic cell-specific mgat2 knockout mice show antigen presentation defects but reveal an unexpected cd11c expression pattern.zwitterionic polysaccharide antigens such as polysaccharide a (psa) from bacteroides fragilis have been shown to activate cd4(+) t cells upon presentation by class ii major histocompatibility complex (mhcii) on professional antigen presenting cells. for t cell recognition and activation, high affinity binding between mhcii and psa is required, and complex n-glycans on conserved mhcii asparagine residues play a central role in controlling this interaction. by truncating these glycans in a myeloid ...201627146521
lactate dehydrogenase activity in bacteroides fragilis group strains with induced resistance to metronidazole.the aims of this study were to induce in vitro metronidazole resistance in nim-negative bacteroides fragilis group strains and to determine the lactate dehydrogenase (ldh) activity of the induced strains. a collection of b. fragilis group strains were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof/ms). minimum inhibitory concentrations (mics) for metronidazole were determined by the agar dilution technique. the presence of nim genes was scre ...201627436459
anaerobic bacteremia: impact of inappropriate therapy on mortality.investigation on incidence and mortality of anaerobic bacteremia (ab) is clinically relevant in spite of its infrequent occurrence and not often explored, which report varies according to period and institutions. therefore, it is necessary to analyze the incidence and risk factors related to mortality and assess clinical outcomes of ab in current aspect.201627433379
efficient and regioselective synthesis of globotriose by a novel α-galactosidase from bacteroides fragilis.globotriose (galα1-4galβ1-4glc) is an important cell surface epitope that acts as the receptor for shiga-like toxins, and it is also the core structure of globo h and ssea4 that are tumor-associated glycans. hence, the enzymatic synthesis of globotriose would be necessary for the development of carbohydrate-based therapeutics for bacterial infections and cancers. here, a novel gh27 α-galactosidase gene (agabf3s), a 1521-bp dna encoding 506 amino acids with a calculated molecular mass of 57.7 kda ...201627020280
effect of oligosaccharides on the adhesion of gut bacteria to human ht-29 cells.the influence of five oligosaccharides (cellobiose, stachyose, raffinose, lactulose and chito-oligosaccharides) on the adhesion of eight gut bacteria (bifidobacterium bifidum atcc 29521, bacteroides thetaiotaomicron atcc 29148d-5, clostridium leptum atcc 29065, blautia coccoides atcc 29236, faecalibacterium prausnitzii atcc 27766, bacteroides fragilis atcc 23745, clostridium difficile atcc 43255 and lactobacillus casei atcc 393) to mucous secreting and non-mucous secreting ht-29 human epithelial ...201627018325
the dysregulation of polyamine metabolism in colorectal cancer is associated with overexpression of c-myc and c/ebpβ rather than enterotoxigenic bacteroides fragilis infection.colorectal cancer is one of the most common cancers in the world. it is well known that the chronic inflammation can promote the progression of colorectal cancer (crc). recently, a number of studies revealed a potential association between colorectal inflammation, cancer progression, and infection caused by enterotoxigenic bacteroides fragilis (etbf). bacterial enterotoxin activates spermine oxidase (smo), which produces spermidine and h2o2 as byproducts of polyamine catabolism, which, in turn, ...201627433286
strain competition restricts colonization of an enteric pathogen and prevents colitis.the microbiota is a major source of protection against intestinal pathogens; however, the specific bacteria and underlying mechanisms involved are not well understood. as a model of this interaction, we sought to determine whether colonization of the murine host with symbiotic non-toxigenic bacteroides fragilis could limit acquisition of pathogenic enterotoxigenic b. fragilis we observed strain-specific competition with toxigenic b. fragilis, dependent upon type vi secretion, identifying an effe ...201627432285
efficient and regioselective synthesis of β-galnac/glcnac-lactose by a bifunctional transglycosylating β-n-acetylhexosaminidase from bifidobacterium bifidum.β-n-acetylhexosaminidases have attracted interest particularly for oligosaccharide synthesis, but their use remains limited by the rarity of enzyme sources , low efficiency, and relaxed regioselectivity of transglycosylation. in this work, genes of 13 β-n-acetylhexosaminidases, including 5 from bacteroides fragilis atcc 25285, 5 from clostridium perfringens atcc 13124, and 3 from bifidobacterium bifidum jcm 1254, were cloned and heterogeneously expressed in escherichia coli the resulting recombi ...201627422836
differential proteomic analysis of outer membrane enriched extracts of bacteroides fragilis grown under bile salts stress.bacteroides fragilis is the most commonly isolated anaerobic bacteria from infectious processes. several virulence traits contribute to the pathogenic nature of this bacterium, including the ability to tolerate the high concentrations of bile found in the gastrointestinal tract (git). the activity of bile salts is similar to detergents and may lead to membrane permeabilization and cell death. modulation of outer membrane proteins (omps) is considered a crucial event to bile salts resistance. the ...201626948242
heterogeneity in resistant fecal bacteroides fragilis group collected from healthy people.normal nonpathogenic flora would represent a constant lake of resistance genes potentially transferable to human pathogens. to assess the prevalence of resistance genes and genetic variability of bacteroides fragilis group (bfg) from normal flora, 177 bacteroides isolates obtained from the fecal samples of healthy individuals. these isolates were subjected to antibiotic susceptibility testing and pulsed field gel electrophoresis (pfge). the isolates were further tested for the presence of ermf, ...201626945562
[how some commensal bacteria would exacerbate colorectal carcinogenesis?].the gut microbiota maintains a relationship with its host with strong mutual benefits. changes in the composition of the intestinal microbiota have been detected in colorectal cancer patients to the extent that it is now considered as a real contributing factor in this pathology. in this review, we focus on three commensal bacterial species, namely bacteroides fragilis, fusobacterium nucleatum, and escherichia coli, which seem to emerge as pathogens and to contribute to colorectal carcinogenesis ...201626936175
reduction of murine colon tumorigenesis driven by enterotoxigenic bacteroides fragilis using cefoxitin treatment.chronic inflammation and composition of the colon microbiota have been associated with colorectal cancer in humans. the human commensal enterotoxigenic bacteroides fragilis (etbf) is linked to both inflammatory bowel disease and colorectal cancer and, in our murine model, causes interleukin 17a (il-17a)-dependent colon tumors. in these studies, we hypothesized that persistent colonization by etbf is required for tumorigenesis.201626908749
multidrug-resistant bacteroides fragilis bacteremia in a us resident: an emerging challenge.we describe a case of bacteroides fragilis bacteremia associated with paraspinal and psoas abscesses in the united states. resistance to b-lactam/b-lactamase inhibitors, carbapenems, and metronidazole was encountered despite having a recent travel history to india as the only possible risk factor for multidrug resistance. microbiological cure was achieved with linezolid, moxifloxacin, and cefoxitin.201627418986
bacteroides fragilis metabolises exopolysaccharides produced by bifidobacteria.bacteroides fragilis is the most frequent species at the human intestinal mucosal surface, it contributes to the maturation of the immune system although is also considered as an opportunistic pathogen. some bifidobacterium strains produce exopolysaccharides (eps), complex carbohydrate polymers that promote changes in the metabolism of b. fragilis when this microorganism grows in their presence. to demonstrate that b. fragilis can use eps from bifidobacteria as fermentable substrates, purified e ...201627418149
gut microbiota imbalance and colorectal cancer.the gut microbiota acts as a real organ. the symbiotic interactions between resident micro-organisms and the digestive tract highly contribute to maintain the gut homeostasis. however, alterations to the microbiome caused by environmental changes (e.g., infection, diet and/or lifestyle) can disturb this symbiotic relationship and promote disease, such as inflammatory bowel diseases and cancer. colorectal cancer is a complex association of tumoral cells, non-neoplastic cells and a large amount of ...201626811603
occurrence of ferredoxin:nad(+) oxidoreductase activity and its ion specificity in several gram-positive and gram-negative bacteria.a ferredoxin:nad(+) oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium acetobacterium woodii. the enzyme is assumed to be encoded by the rnf genes. since these genes are present in the genomes of many bacteria, we tested for ferredoxin:nad(+) oxidoreductase activity in cytoplasmic membranes from several different gram-positive and gram-negative bacteria that have annotated rnf genes. we found this activity in clostridium tetanomorphum, clostr ...201626793417
type vi secretion systems of human gut bacteroidales segregate into three genetic architectures, two of which are contained on mobile genetic elements.type vi secretion systems (t6sss) are contact-dependent antagonistic systems employed by gram negative bacteria to intoxicate other bacteria or eukaryotic cells. t6sss were recently discovered in a few bacteroidetes strains, thereby extending the presence of these systems beyond proteobacteria. the present study was designed to analyze in a global nature the diversity, abundance, and properties of t6sss in the bacteroidales, the most predominant gram negative bacterial order of the human gut.201626768901
efficacy of vancomycin extended-dosing regimens for treatment of simulated clostridium difficile infection within an in vitro human gut model.effects of two vancomycin extended-dosing regimens on microbiota populations within an in vitro gut model of simulated clostridium difficile infection (cdi) were evaluated.201626755495
association of systemic sclerosis with a unique colonic microbial consortium.to compare colonic microbial composition in systemic sclerosis (ssc) patients and healthy controls and to determine whether certain microbial genera are associated with gastrointestinal (gi) tract symptoms in patients with ssc.201626749064
oral administration of synthetic porcine beta-defensin-2 improves growth performance and cecal microbial flora and down-regulates the expression of intestinal toll-like receptor-4 and inflammatory cytokines in weaned piglets challenged with enterotoxigenic escherichia coli.synthetic porcine beta-defensin-2 (pbd-2) was tested as an alternative to antimicrobial growth-promoters in pig production. thirty 21-day weaned piglets were challenged with enterotoxigenic escherichia coli, and orally dosed with either sterile water (con), pbd-2 (bd) or neomycin sulphate (ns) twice daily for 21 days. pbd-2 and ns led to higher growth performance, jejunum villus height and increased expression of insulin-like growth factor-i compared with the con group (p < 0.05). hemolytic e. c ...201626707031
gut microbiota, inflammation, and colorectal cancer.colorectal cancer is the second-leading cause of cancer-related deaths in the united states and fourth-leading cause of cancer-related deaths worldwide. while cancer is largely considered to be a disease of genetic and environmental factors, increasing evidence has demonstrated a role for the microbiota (the microorganisms associated with the human body) in shaping inflammatory environments and promoting tumor growth and spread. herein, we discuss both human data from meta'omics analyses and dat ...201627607555
partially hydrolysed guar gum ameliorates murine intestinal inflammation in association with modulating luminal microbiota and scfa.partially hydrolysed guar gum (phgg), a water-soluble dietary fibre produced by the controlled partial enzymatic hydrolysis of guar gum beans, has various physiological roles. this study aimed to elucidate the beneficial effects of phgg on colonic mucosal damage in a murine 2,4,6-trinitrobenzene sulfonic acid (tnbs)-induced colitis model. acute colitis was induced in male c57bl/6 mice with tnbs after 2 weeks of pre-feeding with phgg (5 %). the colonic mucosal inflammation was evaluated using mac ...201627604176
altered gut microbiota composition associated with eczema in infants.eczema is frequently the first manifestation of an atopic diathesis and alteration in the diversity of gut microbiota has been reported in infants with eczema. to identify specific bacterial communities associated with eczema, we conducted a case-control study of 50 infants with eczema (cases) and 51 healthy infants (controls). we performed high-throughput sequencing for v3-v4 hypervariable regions of the 16s rrna genes from the gut fecal material. a total of 12,386 otus (operational taxonomic u ...201627812181
sialoglycoprotein isolated from the eggs of carassius auratus prevents bone loss: an effect associated with the regulation of gut microbiota in ovariectomized rats.the anti-osteoporotic effect of sialoglycoprotein isolated from the eggs of carassius auratus (ca-sgp) has previously been demonstrated in vivo. however, the mechanism by which this macromolecular substance regulates bone metabolism remains unclear. given the correlation between gut microbiota and the homeostasis of bone metabolism, the current study applied real-time pcr and 16s rrna high-throughput sequencing to investigate the influence of ca-sgp on gut microbiota in ovariectomized rats. real ...201627808338
colon targeted drug delivery of branch-chained disulphide cross-linked polymers: design, synthesis, and characterisation studies.drug delivery directly to the colon is a very useful approach for treating localised colonic diseases such as inflammatory bowel disease, ulcerative colitis, and crohn's disease. the use of disulphide cross-linked polymers in colon targeted drug delivery systems has received much attention because these polymers are redox sensitive, and the disulphide bonds are only cleaved by the low redox potential environment in the colon. the goal of this study was to synthesise tricarballylic acid-based tri ...201627994641
sensitive quantitative analysis of the meconium bacterial microbiota in healthy term infants born vaginally or by cesarean section.for decades, babies were thought to be born germ-free, but recent evidences suggest that they are already exposed to various bacteria in utero. however, the data on population levels of such pioneer gut bacteria, particularly in context to birth mode, is sparse. we herein aimed to quantify such bacteria from the meconium of 151 healthy term japanese infants born vaginally or by c-section. neonatal first meconium was obtained within 24-48 h of delivery; rna was extracted and subjected to reverse- ...201628018325
[design of artificial foetor flatus based on bacterial volatile compounds].excessive flatulence can be a huge social problem. the purpose of this study was to design artificial flatus from bacterial volatile compounds to stimulate research into neutralizing measures.201627966414
erythritol/chlorhexidine combination reduces microbial biofilm and prevents its formation on titanium surfaces in vitro.the purpose of this in vitro study was to evaluate the antibiofilm activity of a novel air-polishing powder consisting of erythritol and chlorhexidine, assessing its ability to reduce previously grown microbial biofilm and to prevent biofilm formation on titanium surfaces.201627935124
anaerobic infections in surgical wards: a two year study.anaerobic bacteria are recognized as important pathogens in surgical infections. however, they are the most overlooked microorganisms by the clinic and the laboratory because of the tedious culture techniques with longer turn-around times. the study was aimed to analyze the frequency of anaerobic bacterial surgical infections and their predisposing factors.201627928485
improved production of 2'-fucosyllactose in engineered escherichia coli by expressing putative α-1,2-fucosyltransferase, wcfb from bacteroides fragilis.2'-fucosyllactose (2-fl) is one of most abundant oligosaccharides in human milk, which is involved in many biological functions for infant health. since 2-fl has a great potential in application to functional food materials and pharmaceuticals, several microbial systems for mass production of 2-fl have been developed in recent years. microbial production of 2-fl was suggested to be influenced by a number of factors including fucosylation activity of α-1,2-fucosyltransferase. in the present study ...201627919692
dna inversion regulates outer membrane vesicle production in bacteroides fragilis.phase changes in bacteroides fragilis, a member of the human colonic microbiota, mediate variations in a vast array of cell surface molecules, such as capsular polysaccharides and outer membrane proteins through dna inversion. the results of the present study show that outer membrane vesicle (omv) formation in this anaerobe is also controlled by dna inversions at two distantly localized promoters, ivp-i and ivp-ii that are associated with extracellular polysaccharide biosynthesis and the express ...201626859882
tissue-associated bacterial alterations in rectal carcinoma patients revealed by 16s rrna community profiling.sporadic and inflammatory forms of colorectal cancer (crc) account for more than 80% of cases. recent publications have shown mechanistic evidence for the involvement of gut bacteria in the development of both crc-forms. whereas, colon and rectal cancer have been routinely studied together as crc, increasing evidence show these to be distinct diseases. also, the common use of fecal samples to study microbial communities may reflect disease state but possibly not the tumor microenvironment. we pe ...201628018861
patient-specific bacteroides genome variants in pouchitis.a 2-year longitudinal microbiome study of 22 patients who underwent colectomy with an ileal pouch anal anastomosis detected significant increases in distinct populations of bacteroides during 9 of 11 patient visits that coincided with inflammation (pouchitis). oligotyping and metagenomic short-read annotation identified bacteroides populations that occurred in early samples, bloomed during inflammation, and reappeared after antibiotic treatment. targeted cultivation of bacteroides isolates from ...201627935837
role of enterotoxigenic bacteroides fragilis in children less than 5 years of age with diarrhea in tabriz, iran.diarrhea is the most frequent health problem among children in developing countries. defining the etiology of acute diarrhea is critical to disease therapy and prevention. some anaerobic bacteria such as enterotoxigenic bacteroides fragilis (etbf) strains cause diarrheal disease by production of enterotoxin in children less than 5 years old.201627635209
hydrocarbon biosynthesis in periplaneta americana. 20165097114
partial resection of the renal pelvis and lower pole of the kidney for hydronephrosis. 20165095572
[treatment of congenital clubfoot]. 20165052749
[therapeutic experience with pentazocine (sosegon)--application to severe pain due to periosteal necrosis of gangrenous rhinitis]. 20165102282
patient care appraisal. iv. 20165078691
[experiences with echoencephalography in diagnostics following skull injuries]. 20165084046
to the editor: coronary artery surgery. 20165073230
[relation between laboratory values and blood volume]. 20165093279
[the diagnosis and surgical treatment of obstructive jaundice]. 20165070355
homemade electrolyte solutions. 20165042506
ophthalmic polymers as ocular wetting agents. 20165009990
gynaecological patients and their nurses. 20165037510
[the function dependant localization of enzymes of energy metabolism in the skeletal muscle of the frog]. 20164992028
[effect of haloperidol on the central nervous system]. 20164971063
[on the fate of sulfur in the course of germination of the pea (pisum sativum l.)]. 20164967522
[ultrastructural changes of the thyrotropic cells of the distal hypophysis of the brown frog after thyroidectomy]. 20164956742
[treatment of osteoarticular manifestations of drepanocytosis with an arteriolar vasodilator agent]. 20165004284
[psychopharmacology in relation to philosophical thought]. 20164927065
[cellular pathology on the histopathological basis and in comparison with diagnostic cytology]. 20164923085
[differential diagnosis of myoclonic akinetic petit mal (lennox's syndrome) and of the myoclonic syndrome observed during fat metabolism disorders]. 20164998333
cornpicker's pupil: jimson weed mydriasis. 20164935919
[experimental study on the relationship between lobectomy and circular resection with plastic reconstruction of the main bronchi]. 20164894039
[cyanosis and anoxia in congenital heart defects]. 20164883796
perception bibliography: lx. psychological abstracts, 1941, volume 15, first half. 20164879479
the cleveland clinic foundation. 20164876462
["rapid" isotopic exploration with iron-59 (najean's technic)]. 20164925910
[letter: tonic water and liver damage]. 20164836077
[structure and function of the nuclear membrane]. 20164899533
[cardiopulmonary reanimation]. 20165053825
[a program for cardiologic screening in gävle]. 20165072455
cysts of the adrenal gland. 20164815108
spleen colony formation in radiation chimeras treated with allopurinol and ampicillin. 20164904575
[psychosomatic aspects of the climacteric]. 20164813281
about ballistocardiographic abnormalities noticed during the evolution of a ventricular aneurysm. 20164802384
dissociation of geographical and histiogenic development of arteries. 20164783014
a scanner for the absorption optical system of the analytical ultracentrifuge based on a vidicon television camera. 20164850289
imipramine in the treatment of minimal brain dysfunction in children. 20164794958
[evaluation of selective coronary angiography]. 20164768707
health planning in the u.s.s.r.: are there lessons to be learned? 20164829075
echocardiographic features of mitral starr-edwards paraprosthetic regurgitation. 20164716046
[report on the occupational environment]. 20164782487
identification of a new lipoprotein export signal in gram-negative bacteria.bacteria of the phylum bacteroidetes, including commensal organisms and opportunistic pathogens, harbor abundant surface-exposed multiprotein membrane complexes (sus-like systems) involved in carbohydrate acquisition. these complexes have been mostly linked to commensalism, and in some instances, they have also been shown to play a role in pathogenesis. sus-like systems are mainly composed of lipoproteins anchored to the outer membrane and facing the external milieu. this lipoprotein localizatio ...201627795390
comparative genomic analysis identifies structural features of crispr-cas systems in riemerella anatipestifer.riemerella anatipestifer infection is a contagious disease that has resulted in major economic losses in the duck industry worldwide. this study attempted to characterize crispr-cas systems in the disease-causing agent, riemerella anatipestifer (r. anatipestifer). the crispr-cas system provides adaptive immunity against foreign genetic elements in prokaryotes and crispr-cas loci extensively exist in the genomes of archaea and bacteria. however, the structure characteristics of r. anatipestifer c ...201627577199
immunity to intracellular bacteria. 20164870271
outpatient surgery. 20164728657
[adenocarcinoma and leukemia induced by mammary tumor culture media in vitro. ultrastructural control]. 20164985230
[luhacovice and the fate of janácek]. 20164936053
half nose. 20164718450
[influence of various analgesics (pethidine, pentazocine and piritramide) on respiration and blood circulation]. 20165050524
queen anne's favourite physician, john arbuthnot, m.d. (1667-1735). 20164860040
coronary arteries--old and new. 20165087496
[rib changes in rheumatoid arthritis]. 20165051949
[evaluation of the sight organ in children born of mothers showing positive reactions to toxoplasmosis]. 20165086476
fractures of the cervical spine with minimal or no symptoms. 20165069413
[vacuolation of skeletal muscle fibers. v. origin of vacuoles during spreading degeneration]. 20165035509
tumor-induced hypercalcemia. 20165033985
rates of oxygen uptake in proliferating and differentiating neuroblastoma cells. 20165025651
infantile handling differentially effects inter-strain dominance interactions in mice. 20165063993
eye movements evoked by stimulation of frontal eye fields. 20164980022
[on dosimetry on the 17-mev betatron]. 20164973539
complications and toxic manifestations of surgical adjuvant chemotherapy for breast cancer. 20164973320
[prostaglandins in the physiology of reproduction]. 20164940397
[gilbert robin (1893-1967)]. 20164876565
[25th anniversary of the institute for psychogenic diseases of the general health insurance agency of berlin]. 20164931636
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