Publications

[the formation techniques of laminate veneers].		2012	3271146
radiographic anatomy of the calcaneus. part iii: superior surface.		2012	3430354
changes in the hair of black patients with aids.		2012	3335818
how canadians can support namda.		2012	3409113
focal dermal hypoplasia syndrome (goltz syndrome): the first dental case report.		2012	3466146
separation and identification of trifluralin metabolites by open-tubular liquid chromatography/negative chemical ionization mass spectrometry.	the utility of combined open-tubular liquid chromatography/negative ion chemical ionization (nci) mass spectrometry for the separation and analysis of trifluralin, a commonly used pre-emergence herbicide, and seven of its metabolites has been demonstrated. the nci mass spectra for these compounds are reported. some of the nci mass spectra were obtained from as little as 50 pg of analyte.	2011	3203148
[dynamics of the excitability of the cardiovascular centers in changes in disturbances of the geomagnetic field].		2011	3169412
lessons from a nursing home.		2011	3103447
[preliminary study on the diagnosis of active tuberculosis by using the abc-elisa method].		2011	3197169
foreperiod duration and the analysis of motor stages in a line-drawing task.		2011	3245475
foreperiod duration and the analysis of motor stages in a line-drawing task.		2011	3245475
[rhabdomyolysis in a severe form of parkinson's disease].		2011	3160023
cyclophosphamide-induced liver necrosis: a possible interaction with azathioprine.	cyclophosphamide is a valuable drug for the treatment of vasculitis and hepatic toxicity resulting from its use has been reported rarely. we describe four patients in whom cyclophosphamide was associated with liver injury when its administration had been preceded by azathioprine. microscopy of hepatic biopsy material in three of these cases showed liver cell necrosis. in two of the patients, cyclophosphamide had been given previously without earlier azathioprine and hepatic damage had not been n ...	2011	3060893
progress of a harlequin fetus to nonbullous ichthyosiform erythroderma.	the term harlequin fetus is used to describe the most severe form of congenital ichthyosis. all except one previously reported patient died during the first few weeks of life. the development of one such fetus to the age of 2 years 6 months is reported, along with a photographic record of progress. details of the progress of another harlequin fetus to 6 months of age are given. the clinical diagnosis of the former patient is nonbullous ichthyosiform erythroderma.	2011	3186377
nita interviews cynthia chrystal, r.n.		2011	3923406
nita interviews cynthia chrystal, r.n.		2011	3923406
investigations on the relative synthesis of globin chains in thalassaemia: a preliminary study in malaysian subjects.		2011	3241975
left aortic arch with right descending aorta.		2011	3202754
the costs of crime and the benefits of drug abuse treatment: a cost-benefit analysis using tops data.		2011	3140032
prostacyclin, edrf and atherosclerosis.		2011	3066171
serum free thyroxine and free tri-iodothyronine in normal children.	serum free thyroxine (ft4) and free tri-iodothyronine (ft3) were measured in 138 normal school children, adolescents and their younger siblings, whose ages ranged from 3 months to 18 years. mean ft4 concentration (16.8 pmol/l) was similar to the adult mean concentration of 17 pmol/l and all the values were within the adult reference range. at all ages the ft3 range was considerably higher than the adult reference range and the overall mean ft3 concentration (8.3 pmol/l) was at the upper limit of ...	2011	3232955
lectin histochemistry in colorectal polyps.	lectin histochemistry has permitted an assessment of the glycoconjugate expression in colorectal polyps (table 8). the t-antigen, a cancer-associated antigen, is expressed in colorectal adenomatous polyps. the apparent expression of t-antigen is related to the methodology used for its detection. fitc-pna does not bind to glycoconjugates in the normal colon, however it is a sensitive marker for glycoconjugates secreted by colorectal cancers. the binding of fitc-pna in adenomatous polyps is relate ...	2011	3186774
interactions of age, gender, and sensorineural hearing loss on abr latency.	the interactive effects of gender, age, and degree of sensorineural hearing loss on the absolute latency of wave v of the auditory brain stem response were analyzed in 325 subjects with cochlear loss. norms based on these data were then applied to an analysis of the wave v latencies of 87 subjects with retrocochlear loss. results in the cochlear group revealed interactions between gender and age and between gender and degree of hearing loss. the interactive effect between gender and degree of lo ...	2011	3169397
nutritional assessment in a surgical practice.		2011	3131063
intravenous indomethacin for postoperative pain. a double-blind study of ankle surgery.	intravenous indomethacin infusion (75 mg/10 h) for relief of pain after ankle surgery was compared with placebo in a double-blind study. ninety-seven patients were operated on for a malleolar fracture or ruptured ligament of the ankle under single-dose spinal anesthesia. in the indomethacin group, 14 out of 49 patients were free from pain, 4/47 in the control group. severe pain was experienced by 24/49 in the indomethacin group and by 41/47 in the control group. supplementary intramuscular oxyco ...	2011	3128054
quality assurance in hospice care.		2011	3671406
[optimization of nutrition for premature infants using essential fatty acids].		2011	3105029
[echographic and radiographic aspects of a case of burkitt's lymphoma].		2011	3060909
biased adverse effects?		2011	3173451
[x-ray computed tomography in venous pathology].	after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed.	2011	3043474
[primary transport of acute patients. optimal use of rescue resources exemplified by the canton of graubünden].		2011	3927607
when is serum albumin worth measuring?		2011	3214132
bacterial genome chimaerism and the origin of mitochondria.	many studies have sought to determine the origin and evolution of mitochondria. although the alphaproteobacteria are thought to be the closest relatives of the mitochondrial progenitor, there is dispute as to what its particular sister group is. some have argued that mitochondria originated from ancestors of the order rickettsiales, or more specifically of the rickettsiaceae family, while others believe that ancestors of the family rhodospirillaceae are also equally likely the progenitors. to re ...	2011	21217797
characterization of rickettsial adhesin adr2 belonging to a new group of adhesins in a-proteobacteria.	rickettsia prowazekii is the etiological agent of epidemic typhus and is an obligate intracellular bacterium that grows as a parasite freely within the cytoplasm of a eukaryotic host cell. previous studies have shown that rompa and rompb which belong to the family of rickettsial cell surface antigens are involved in vitro in the adhesion of rickettsiae to epithelial cells. recently, two putative rickettsial adhesins have been identified using high resolution 2d-page coupled with mass spectrometr ...	2011	21288480
analysis of convergent gene transcripts in the obligate intracellular bacterium rickettsia prowazekii.	termination of transcription is an important component of bacterial gene expression. however, little is known concerning this process in the obligate intracellular pathogen and model for reductive evolution, rickettsia prowazekii. to assess transcriptional termination in this bacterium, transcripts of convergent gene pairs, some containing predicted intrinsic terminators, were analyzed. these analyses revealed that, rather than terminating at a specific site within the intervening region between ...	2011	21298070
[louse-borne infections in humans].		2011	21450425
[an outbreak of endemic typhus in baoshan city, yunnan province.]	objective: to understand the epidemiologic characteristics of endemic typhus in baoshan city. methods: epidemiological data were collected and characteristics were analyzed. igg antibody (ab) of rickettsia mooseri and orientia tsutsugamushi in serum of patients were tested using both weil-felix and ifa method. the rickettsia mooseri glta gene, rickettsia prowazekii glta gene, orientia tsutsugamushi 56 kda protein gene, sfgr ompa gene, ehrlichia sp. 16s rrna gene and anaplasma sp. 16s rrna gene i ...	2011	21518541
host, pathogen and treatment-related prognostic factors in rickettsioses.	diseases caused by rickettsiae, which are vector-borne bacteria, vary widely from mild and self-limiting, to severe and life-threatening. factors influencing this diversity of outcome are related to the host, to the infectious agent and to the treatment used to treat the infection. a literature search was conducted on pubmed using the phrases "factors-related severity, outcome, host, pathogen, rickettsia conorii, r. rickettsii, r. africae, r. felis, r. prowazekii, r. typhi, genomics". among host ...	2011	21519943
differential proteomic analysis of rickettsia prowazekii propagated in diverse host backgrounds.	the obligate intracellular growth of rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. with a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae isolated from different hosts or that vary in virulence, is critical to an understanding of rickettsial pathogenicity. we employed an lc-ltq-orbitrap mass spectrometer for the simultaneous acquisition of quantitative ms-only data and tand ...	2011	21642410
the rickettsia prowazekii exou homologue possesses phospholipase a1 (pla1), pla2, and lyso-pla2 activities and can function in the absence of any eukaryotic co-factors in vitro.	here we have characterized the rickettsia prowazekii rp534 protein; a homologue of the pseudomonas aeruginosa exou phospholipase a (pla) secreted cytotoxin. our studies showed that purified recombinant rp534 pla possessed the predicted pla(2) and lyso-pla(2) activities based on what has been published for p. aeruginosa exou. rp534 also displayed pla(1) activity under the conditions tested whereas exou did not. in addition, recombinant rp534 displayed a basal pla activity that could hydrolyze pho ...	2011	21764940
transformation frequency of a mariner-based transposon in rickettsia rickettsii.	transformation frequencies of a mariner-based transposon system in rickettsia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants. sequence analysis of insertion sites from both r. rickettsii and r. prowazekii indicated that insertions were random. transposon mutagenesis provides a useful tool for rickettsial research.	2011	21764933
Structure of fumarate hydratase from Rickettsia prowazekii, the agent of typhus and suspected relative of the mitochondria.	Rickettsiae are obligate intracellular parasites of eukaryotic cells that are the causative agents responsible for spotted fever and typhus. Their small genome (about 800 protein-coding genes) is highly conserved across species and has been postulated as the ancestor of the mitochondria. No genes that are required for glycolysis are found in the Rickettsia prowazekii or mitochondrial genomes, but a complete set of genes encoding components of the tricarboxylic acid cycle and the respiratory-chai ...	2011	21904061
development of a duplex real-time pcr for the detection of rickettsia spp. and typhus group rickettsia in clinical samples.	molecular diagnosis using real-time polymerase chain reaction (pcr) may allow earlier diagnosis of rickettsiosis. we developed a duplex real-time pcr that amplifies (1) dna of any rickettsial species and (2) dna of both typhus group rickettsia, that is, rickettsia prowazekii and rickettsia typhi. primers and probes were selected to amplify a segment of the 16s rrna gene of rickettsia spp. for the pan-rickettsial pcr and the citrate synthase gene (glta) for the typhus group rickettsia pcr. analyt ...	2011	22098502
protein candidates for the serodiagnosis of rickettsioses.	the laboratory diagnosis of rickettsioses is based on serology (reference method), cell culture and/or molecular tools. however, the main drawback of serology is its incapacity to provide identification of rickettsiae at the level of species. the aim of this study was to propose the versatile protein markers able to discriminate the patients with murine typhus from those with mediterranean spotted fever. we have cloned and expressed 20 proteins of rickettsia prowazekii and rickettsia rickettsii, ...	2011	22098357
structure of 3-ketoacyl-(acyl-carrier-protein) reductase from rickettsia prowazekii at 2.25 å resolution.	rickettsia prowazekii, a parasitic gram-negative bacterium, is in the second-highest biodefense category of pathogens of the national institute of allergy and infectious diseases, but only a handful of structures have been deposited in the pdb for this bacterium; to date, all of these have been solved by the ssgcid. owing to its small genome (about 800 protein-coding genes), it relies on the host for many basic biosynthetic processes, hindering the identification of potential antipathogenic drug ...	2011	21904060
[accuracy of fit of castings in relation to impression, model and technical parameters].		2011	3044749
tripelennamine enhances buprenorphine-, but not pentazocine-induced hyperactivity in mice.	the opioid agonist-antagonist pentazocine (1-30 mg/kg) and the partial agonist buprenorphine (0.05-0.25 mg/kg) were tested, alone or in combination with the histamine h1-receptor antagonist tripelennamine (1, 2.5, 5 or 10 mg/kg), on locomotor activity in mice of the cd-1 strain. when given alone, pentazocine produced slight and non-dose-related activity increments, while buprenorphine induced strong and dose-related locomotor stimulation. tripelennamine slightly increased activity by itself and ...	2011	3137594
ovarian carcinoma: abdominopelvic irradiation following reexploration.	twenty patients with ovarian cancer, who following reexploration were left with residual disease nodules of less than 2 cm diameter, received abdominopelvic irradiation. of these patients 18 had previous chemotherapy. fifteen patients completed treatment, 13 of whom had prior chemotherapy. six of the 18 patients with invasive tumors were alive and disease free 18-53 months postradiotherapy, while only 4 patients had died at the time of follow-up. no patient with residual nodules greater than 1 c ...	2011	3141249
clinical evaluation of ocular and visual functions in insulin-dependent juvenile diabetics.	ocular and visual functions were evaluated in 30 insulin-dependent diabetics, aged 12 to 20 years, and 30 age- and sex-matched nondiabetics. the test and control groups were compared in terms of visual resolution, accommodative ability, color discrimination, functional retinal reserve, and afferent optic nerve function. these results were considered along with the blood-glucose level, crystalline lens, fundus appearance, level of diabetic control, and disease duration. no widespread differences ...	2011	3740205
lipid synthesis and deposition by adult richardson's ground squirrels in the natural environment.	adult male richardson's ground squirrels, spermophilus richardsonii, were estimated to have emerged from hibernation in late february to early march, and adult females in mid to late march. half of the females trapped in late march were not pregnant, as against 10% after that time. in late march males and all females had similar wat (white adipose tissue) deposits. between late march and early june, wat deposits in males increased from 14 g to 64 g (a rate of 5.6 g per week). in non-parous femal ...	2011	3170826
lipid synthesis and deposition by adult richardson's ground squirrels in the natural environment.	adult male richardson's ground squirrels, spermophilus richardsonii, were estimated to have emerged from hibernation in late february to early march, and adult females in mid to late march. half of the females trapped in late march were not pregnant, as against 10% after that time. in late march males and all females had similar wat (white adipose tissue) deposits. between late march and early june, wat deposits in males increased from 14 g to 64 g (a rate of 5.6 g per week). in non-parous femal ...	2011	3170826
report of the second public health service aids prevention and control conference. women and aids.		2011	3147491
present criteria for the diagnosis of in vivo of olivopontocerebellar atrophy.	we present 6 cases of olivopontocerebellar atrophy (opca) of dèjérine-thomas type. the diagnosis was based on an appropriate clinical picture, anomalous brainstem auditory evoked potentials (baep) and computerized tomography (ct) and/or magnetic nuclear resonance (mnr) imaging which demonstrated atrophy of the cerebellum and the pons. we believe that these methods will provide greater uniformity in the diagnosis of such an heterogeneous entity as opca.	2011	3163874
sequential ballistic movement.		2011	3208734
phenotypic characterization and genetic analysis of twenty dominant cataract mutations detected in offspring of irradiated male mice.		2011	3209064
neurobehavioral estimation of children with life-long increased lead exposure.	a battery of neurobehavioral examinations was carried out on 30 children who were 6-11 yr of age and who had resided near a lead smelter all their lives. their blood lead levels were 35-60 micrograms/100 ml and erythrocyte protoporphyrin levels were greater than 100 micrograms/100 ml. neurological examination revealed that they had a significantly higher incidence of pathological findings (e.g., muscle hypotonia, increased tendon reflexes, dysarthria, and dysdiadochokinesia) than children from a ...	2011	3196074
[structural analysis of parameters of neurophysiological processes].		2011	3169413
[hyperuricemia in the joint syndrome in children].		2011	3186400
[recommendations for the nomenclature of psoriasis. revised version].		2011	3169327
delayed healing of fractures of the mandibular body.	401 mandibular body fractures occurring during a 5-year period were analysed retrospectively. out of these, 38 fractures (9.5%) were not consolidated by 50 days and made up the delayed healing group (dhg). a control group (cg) of another 38 fractures was constituted using the first mandibular body fracture consecutively following one in the dhg. the mean time until consolidation of the fractures was 116 days in the dhg and 35 days in the cg. the 2 groups were statistically analysed and mutually ...	2011	3104491
proposed reference chart in paediatric resuscitation.		2011	3144386
effect of the energy source on changes in energy expenditure, respiratory quotient, and nitrogen balance during total parenteral nutrition in children.	the effects of three isocaloric intravenous nutritional regimens were studied in seven infants and children, ages 2 months to 9 yr, with congenital gastrointestinal anomalies (four patients) or with prior history of malignant disease admitted in remission for bone marrow transplantation (three patients). energy metabolism, as measured by the basal metabolic rate (bmr), and substrate utilization, as measured by the respiratory quotient (rq), were studied to determine the effect of different level ...	2011	3110723
[the logic of integrating health and social care].		2011	3242838
[assessment and therapy in the clinic in repeated abortion].		2011	3053355
multilineage cell involvement in ph1-negative, bcr-negative chronic myeloid leukemia.	we report a case of ph1-negative, bcr-negative cml-bc, in which the primary leukemic cells displayed t-related antigens (cd7, cd4) in addition to hla-dr and cd25 determinants. no b-lymphoid, myeloid and megakaryoblastic surface antigens were detected. in spite of this phenotype, dna analysis revealed a germ-line configuration of the t-cell receptor beta chain gene region. moreover, in-vitro culture studies demonstrated a proliferative response of the blast cell population to natural and recombin ...	2011	3263550
[general principles of elaboration of methods of drug ultraphonophoresis and their violation in clinical practice].		2011	3212954
further evidence against dopamine deficiency as the cause of inappropriate gonadotropin secretion in patients with polycystic ovary syndrome.	previous studies suggested that a relative dopamine (da) deficiency may explain the altered lh secretory dynamics that occur in patients with polycystic ovary syndrome (pco). these studies included findings of decreased urinary excretion of homovanillic acid (hva), a metabolite of da, and increased urinary excretion of 3-methoxy-4-hydroxyphenylglycol (mhpg), the major brain metabolite of norepinephrine. to further explore the role of da in these patients, disulfiram (250 mg) was administered dai ...	2011	3117832
elevated blood pressure during pregnancy.		2011	3081538
prostatic carcinoma: complications of megavoltage radiation therapy.	megavoltage radiation therapy and radical prostatectomy are at present the only curative treatments for prostatic carcinoma. because a high complication rate appeared to follow radiotherapy, all patients with prostatic carcinoma presenting to a danish provincial hospital between 1975 and 1980 were reviewed. of 31 radiation-treated patients, 23 had serious complications. no recurrence was noted in eight patients. twenty-three had a recurrence despite radiotherapy but 11 of these patients had no l ...	2011	3081075
total body potassium in bartter's syndrome before and during treatment with enalapril.	total body potassium (tbk) was measured by whole body counting of 40k in 3 patients with bartter's syndrome before, after 3 months and after 1 year of treatment with enalapril. in 2 patients tbk was found to be decreased before treatment, whereas tbk was within the normal range in the 3rd. during treatment serum potassium concentration and tbk increased in each subject and symptoms of fatigue and tetany disappeared. enalapril is shown to be an effective treatment in bartter's syndrome as it impr ...	2011	3031521
free intermingling of mammalian beta-tubulin isotypes among functionally distinct microtubules.	mammalian cells express a spectrum of tubulin isotypes whose relationship to the diversity of microtubule function is unknown. to examine whether different isotypes are segregated into functionally distinct microtubules, we generated immune sera capable of discriminating among the various naturally occurring beta-tubulin isotypes. cloned fusion proteins encoding each isotype were used first to tolerogenize animals against shared epitopes, and then as immunogens to elicit a specific response. in ...	2011	3552250
regulation of actomyosin atpase in smooth muscle.	actomyosin in smooth muscle is in a quiescent state. the mechanism or mechanisms by which ca2+ activates the actomyosin atpase is not clear. there is sufficient evidence for the presence of enzyme systems which phosphorylate and dephosphorylate myosin light chains. the activity of the kinase that phosphorylates the myosin is regulated by camp-dependent protein kinase. phosphorylated kinase has decreased affinity for calmodulin and lower activity when compared with unphosphorylated myosin light c ...	2010	2947244
jerzy modrakowski 1875-1945.		2010	3321909
a clinical trial to compare the effect of two antiseptic mouthwashes on gingival inflammation.	a trial was made comparing the effects of efficient and thorough removal of plaque and debris alone, and with a daily antiseptic mouthwash. the results showed considerable variation but no significant difference in gingival inflammation between hygienic treatment and a daily mouthwash with chlorhexidine-gluconate or povidone-iodine. povidone-iodine caused less staining of the teeth than chlorhexidine-gluconate.	2010	2860167
[diagnostic and therapeutic strategy of bilateral nephroblastoma (apropos of 4 cases)].	four cases of bilateral nephroblastoma out of a total number of 99 cases have been observed during a period of 18 years at the inse of tunis (4%). the frequency of associated anomalies, the familial incidence, the young age of the patients are underlined. the main therapeutic modalities are discussed. based on the most recent studies published in the literature, the prognosis of bilateral nephroblastoma has been improved in recent years with a possibility of survival at 2 years exceeding two thi ...	2010	2834692
[injuries of the pancreas].	trauma of the pancreas was studied in 48 patients. it has a typical mechanisms and is characterized by intensity of the exerted force. due to the last named circumstance, extensive retroperitoneal hematomas, ruptures of the parenchymatous organs, ligaments, veins, and large arteries often occur a severe condition of the patients with fall of arterial pressure and the development of shock is characteristic. it is difficult to establish the diagnosis of trauma of the pancreas before the operation. ...	2010	2811136
affinity purification of hexosaminidases.	hexosaminidases a and b were purified by affinity chromatography from normal gastric mucosa, after preliminary separation of isozymes by anion exchange chromatography. heparin and mannosamine were coupled to sepharose 4b and used as affinity matrices and the purified enzymes were found to be homogeneous when analysed by polyacrylamide slab gel electrophoresis. this combination of 2 novel affinity chromatographic procedures is superior to existing methods in that a final yield of over 70% could b ...	2010	2965176
possible role of insulin deficiency and growth hormone in severe retinopathy.		2010	2863521
diet in the etiology of cancer.		2010	3006202
[a clinical study of channel distribution of vitiligo].		2010	2949877
[the polymerase chain reaction].		2010	2797295
pharmacokinetics of postoperative intravenous indomethacin in children.	the disposition of indomethacin was studied in children aged one to four years. indomethacin 0.35 mg/kg was administered as an intravenous infusion during 15 min. venous blood samples were collected until 24 hr after infusion. serum indomethacin was determined with gas chromatography. using a non-linear regression analysis, the individual data were fitted by a two-compartment open mammillary model with central elimination. calculated pharmacokinetic parameters were (mean +/- sd); alpha half-life ...	2010	2813287
temporal lobe pathology in schizophrenia: a quantitative magnetic resonance imaging study.	although numerous studies have confirmed the presence of larger cerebral ventricles in schizophrenia, the locus of tissue loss remains elusive. by analyzing magnetic resonance scans with computerized image analysis, the authors determined gray and white matter volumes in the temporal lobes and prefrontal regions of 17 patients with schizophrenia and 17 age- and sex-matched normal subjects. the volume of temporal lobe gray matter was 20% smaller in the patients than in the control subjects. the l ...	2010	2929746
[endodontic approach to injured teeth].		2010	2897345
[usefulness and side effects of a diet rich in vegetable fiber in insulin-dependent diabetic adolescents].	the influence on metabolic control and the possible side effects of a diet rich in vegetable fibers was evaluated in a group of insulin-dependent diabetic adolescents. after two weeks of controlled diet, a new dietary regime with a higher vegetable fiber content (66 g/day vs 15 g/day) and an increased iron content (22.5 mg/day vs 11.5 mg/day) was assigned to each patient for a period of two months. at the end of the dietary trial, a decrease of the insulin requirement, of the mean daily blood gl ...	2010	2832352
first-trimester prenatal diagnosis for huntington's disease with dna probes.	polymorphic dna probes linked to the locus for huntington disease (hd) were used for prenatal diagnosis of a 10-week fetus at 25% risk for the disease. the fetus proved to have a 48% risk of having inherited the hd mutation which was similar to that for the at-risk parent (50%). on this basis the parents elected to terminate the pregnancy. when appropriate family members are available and dna studies are informative, prenatal diagnosis of hd with polymorphic dna probes can determine the at-risk ...	2010	2884412
adipose tissue serves as a reservoir for recrudescent rickettsia prowazekii infection in a mouse model.	brill-zinsser disease, the relapsing form of epidemic typhus, typically occurs in a susceptible host years or decades after the primary infection; however, the mechanisms of reactivation and the cellular reservoir during latency are poorly understood. herein we describe a murine model for brill-zinsser disease, and use pcr and cell culture to show transient rickettsemia in mice treated with dexamethasone >3 months after clinical recovery from the primary infection. treatment of similarly infecte ...	2010	20049326
review of microarray studies for host-intracellular pathogen interactions.	obligate intracellular bacteria are privileged soldiers on the battlefield that represent host-pathogen interactions. microarrays are a powerful technology that can increase our knowledge about how bacteria respond to and interact with their hosts. this review summarizes the limitations inherent to host-pathogen interaction studies and essential strategies to improve microarray investigations of intracellular bacteria. we have compiled the comparative genomic and gene expression analyses of obli ...	2010	20188126
genomic, proteomic, and transcriptomic analysis of virulent and avirulent rickettsia prowazekii reveals its adaptive mutation capabilities.	rickettsia prowazekii, the agent of epidemic typhus, is an obligate intracellular bacterium that is transmitted to human beings by the body louse. several strains that differ considerably in virulence are recognized, but the genetic basis for these variations has remained unknown since the initial description of the avirulent vaccine strain nearly 70 yr ago. we use a recently developed murine model of epidemic typhus and transcriptomic, proteomic, and genetic techniques to identify the factors a ...	2010	20368341
finishing genomes with limited resources: lessons from an ensemble of microbial genomes.	while new sequencing technologies have ushered in an era where microbial genomes can be easily sequenced, the goal of routinely producing high-quality draft and finished genomes in a cost-effective fashion has still remained elusive. due to shorter read lengths and limitations in library construction protocols, shotgun sequencing and assembly based on these technologies often results in fragmented assemblies. correspondingly, while draft assemblies can be obtained in days, finishing can take man ...	2010	20398345
rickettsia prowazekii uses an sn-glycerol-3-phosphate dehydrogenase and a novel dihydroxyacetone phosphate transport system to supply triose phosphate for phospholipid biosynthesis.	rickettsia prowazekii is an obligate intracellular pathogen that possesses a small genome and a highly refined repertoire of biochemical pathways compared to those of free-living bacteria. here we describe a novel biochemical pathway that relies on rickettsial transport of host cytosolic dihydroxyacetone phosphate (dhap) and its subsequent conversion to sn-glycerol-3-phosphate (g3p) for synthesis of phospholipids. this rickettsial pathway compensates for the evolutionary loss of rickettsial glyc ...	2010	20581209
brill-zinsser disease in a patient following infection with sylvatic epidemic typhus associated with flying squirrels.	recrudescent rickettsia prowazekii infection, also known as brill-zinsser disease, can manifest decades after untreated primary infection but is rare in contemporary settings. we report the first known case of brill-zinsser disease in a patient originally infected with a zoonotic strain of r. prowazekii acquired from flying squirrels.	2010	20687836
100th anniversary of the death of ricketts: howard taylor ricketts (1871-1910). the namesake of the rickettsiaceae family.	the us american pathologist and microbiologist howard taylor ricketts died 100 years ago. he is renowned for discovering the causative organism and the transmission route of rocky mountain spotted fever and of tabardillo--an epidemic louse-borne typhus occurring especially in mexico. he also found that both diseases were caused by related infectious agents (rickettsia rickettsii and rickettsia prowazekii). the scientific community therefore named both a taxonomic family (rickettsiaceae) and an o ...	2010	20888424
[antibacterial antibodies in human immunoglobulins and sera: past and present].	to measure levels of several types of antibacterial antibodies in preparations of normal human immunoglobulin as well as in samples of donor sera obtained in 1965 and 2009.	2010	21061576
leukotriene d4-induced homologous desensitization in basal and differentiated u-937 cells: characterization with the partial agonist leukotriene e4 and assessment of receptor reserve.	u-937 cells express receptors for leukotriene d4, and ltd4 stimulates receptor-mediated ca++ mobilization with an ec50 of 5 nm. in these cells, lte4 produces ca++ mobilization with an ec50 of 500 nm and a maximum response that is 15 to 20% of the ltd4 response. lte4 was shown to be a partial agonist, with a kp of 400 nm and a linear relationship between effect and receptor occupancy, and was used as a tool to study ltd4-induced desensitization. pretreatment of u-937 cells with ltd4 resulted in a ...	2010	2845060
bis-(diethyldithiocarbamato) copper complex: a new metabolite of disulfiram?		2010	2992526
the antineoplastic effect of methylprednisolone pulse therapy in two patients with glucocorticoid receptor-positive glioblastoma multiforme.	glucocorticoids in high concentrations inhibit in vitro the growth rate of glioblastoma multiforme. this presupposes the presence of glucocorticoid receptors in the cytoplasma. glucocorticoid receptor positive patients with histological glioblastoma multiforme were postoperatively treated with high dose methylprednisolone pulse therapy during which ct-scanning initially demonstrated tumor regression. one patient's tumor size was unchanged after 24 weeks, whereas in the other patient, the tumor s ...	2010	2833058
metaphyseal dysostosis schmidt type.		2010	3251840
somatostatin octapeptide (sms 201-995) in the medical treatment of acromegaly.	parenteral somatostatin has been used to suppress growth hormone secretion in acromegalic patients, but long-term treatment is hampered by its short half-life of a few minutes in the circulation. an octapeptide analogue of somatostatin (sms 201-995) has recently been developed that has greater potency and selectivity in suppressing growth hormone than the native hormone. in this study somatostatin and somatostatin octapeptide infusions were compared in 13 patients with active acromegaly. the oct ...	2010	2876497
the acth stimulation test in depression.		2010	3037614