Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| class enzyme-based motors for "on the fly" enantiomer analysis of amino acids. | here, two class-enzyme motors are properly designed allowing the rapid dispersion of the class-enzyme d-amino acid oxidase (dao) and l-amino acid oxidase (lao) for selective "on the fly" biodetection of d and l-amino acids (aas), respectively. the efficient movement together with the continuous release of fresh class-enzyme leads to a greatly accelerated enzymatic reaction processes without the need of external stirring or chemical and physical attachment of the enzyme. ultra-fast detection (<2m ... | 2017 | 28505561 |
| sialylation facilitates self-assembly of 3d multicellular prostaspheres by using cyclo-rgdfk(tpp) peptide. | prostaspheres-based three dimensional (3d) culture models have provided insight into prostate cancer (pca) biology, highlighting the importance of cell-cell interactions and the extracellular matrix (emc) in the tumor microenvironment. although these 3d classical spheroid platforms provide a significant advance over 2d models mimicking in vivo tumors, the limitations involve no control of assembly and structure with only limited spatial or glandular organization. here, matrix-free prostaspheres ... | 2017 | 28496342 |
| recommendations of the advisory committee on immunization practices for use of cholera vaccine. | cholera, caused by infection with toxigenic vibrio cholerae bacteria of serogroup o1 (>99% of global cases) or o139, is characterized by watery diarrhea that can be severe and rapidly fatal without prompt rehydration. cholera is endemic in approximately 60 countries and causes epidemics as well. globally, cholera results in an estimated 2.9 million cases of disease and 95,000 deaths annually (1). cholera is rare in the united states, and most u.s. cases occur among travelers to countries where c ... | 2017 | 28493859 |
| detection of cholera toxin by an immunochromatographic test strip. | as cholera toxin (ct) is responsible for most of the symptoms induced by vibrio cholerae o1 or o139 infection, detection of ct is an important biomarker for diagnosis of the disease. the procedure for pathogenicity analysis of v. cholerae isolates must be carefully developed for the reason that the amount of ct produced by v. cholerae varies according to the medium used and culture conditions (i.e. temperature and aeration status) applied. here we describe a reproducible rapid method for analysi ... | 2017 | 28478552 |
| bile salts and alkaline ph reciprocally modulate the interaction between the periplasmic domains of vibrio cholerae toxr and toxs. | toxr is a transmembrane transcription factor that is essential for virulence gene expression and human colonization by vibrio cholerae. toxr requires its operon partner toxs, a periplasmic integral membrane protein, for full activity. these two proteins are thought to interact through their respective periplasmic domains, toxrp and toxsp. in addition, toxr is thought to be responsive to various environmental cues, such as bile salts and alkaline ph, but how these factors influence toxr is not ye ... | 2017 | 28464377 |
| glucose-specific enzyme iia of the phosphoenolpyruvate:carbohydrate phosphotransferase system modulates chitin signaling pathways in vibrio cholerae. | in vibrio cholerae, the genes required for chitin utilization and natural competence are governed by the chitin-responsive two-component system (tcs) sensor kinase chis. in the classical tcs paradigm, a sensor kinase specifically phosphorylates a cognate response regulator to activate gene expression. however, our previous genetic study suggested that chis stimulates the non-tcs transcriptional regulator tfos by using mechanisms distinct from classical phosphorylation reactions (s. yamamoto, j. ... | 2017 | 28461445 |
| determinants of severe dehydration from diarrheal disease at hospital presentation: evidence from 22 years of admissions in bangladesh. | to take advantage of emerging opportunities to reduce morbidity and mortality from diarrheal disease, we need to better understand the determinants of life-threatening severe dehydration (sd) in resource-poor settings. | 2017 | 28448489 |
| treatment with entinostat heals experimental cholera by affecting physical and chemical barrier functions of intestinal epithelia. | we have shown previously that oral treatment with sodium butyrate or phenylbutyrate in an experimental model of shigellosis improves clinical outcomes and induces the expression of the antimicrobial peptide cap-18 in the large intestinal epithelia. in a subsequent study, we found that entinostat, an aroylated phenylenediamine compound, has similar therapeutic potential against shigellosis. in this study, we aimed to evaluate entinostat as a potential candidate for host-directed therapy against c ... | 2017 | 28438947 |
| structure and function of the divalent anion/na(+) symporter from vibrio cholerae and a humanized variant. | integral membrane proteins of the divalent anion/na(+) symporter (dass) family translocate dicarboxylate, tricarboxylate or sulphate across cell membranes, typically by utilizing the preexisting na(+) gradient. the molecular determinants for substrate recognition by dass remain obscure, largely owing to the absence of any substrate-bound dass structure. here we present 2.8-å resolution x-ray structures of vcindy, a dass from vibrio cholerae that catalyses the co-transport of na(+) and succinate. ... | 2017 | 28436435 |
| the iron chaperone protein cyay from vibrio cholerae is a heme-binding protein. | cyay is an iron transport protein for iron-sulfur (fe-s) cluster biosynthetic systems. it also transports iron to ferrochelatase that catalyzes insertion of fe(2+) into protoporphyrin ix. here, we find that cyay has the ability to bind heme as well as iron, exhibiting an apparent dissociation constant for heme of 21 ± 6 nm. absorption and resonance raman spectra revealed that both ferric and ferrous forms of heme were bound to an anionic ligand (e.g., tyrosine and/or cysteine). consistent with t ... | 2017 | 28436221 |
| modulation of the multidrug efflux pump emrd-3 from vibrio cholerae by allium sativum extract and the bioactive agent allyl sulfide plus synergistic enhancement of antimicrobial susceptibility by a. sativum extract. | the causative agent of cholera, vibrio cholerae, is a public health concern. multidrug-resistant v. cholerae variants may reduce chemotherapeutic efficacies of severe cholera. we previously reported that the multidrug efflux pump emrd-3 from v. cholerae confers resistance to multiple structurally distinct antimicrobials. medicinal plant compounds are potential candidates for emrd-3 efflux pump modulation. the antibacterial activities of garlic allium sativum, although poorly understood, predicts ... | 2017 | 28432381 |
| crystal structure of the regulatory domain of aphb from vibrio vulnificus, a virulence gene regulator. | the transcriptional activator aphb has been implicated in acid resistance and pathogenesis in the food borne pathogens vibrio vulnificus and vibrio cholerae. to date, the full-length aphb crystal structure of v. cholerae has been determined and characterized by a tetrameric assembly of aphb consisting of a dna binding domain and a regulatory domain (rd). although acidic ph and low oxygen tension might be involved in the activation of aphb, it remains unknown which ligand or stimulus activates ap ... | 2017 | 28427249 |
| antimicrobial resistance and genetic diversity of the sxt element in vibrio cholerae from clinical and environmental water samples in northeastern thailand. | multidrug resistance in v. cholerae has been increasing around the world including northeastern thailand. the aquatic environment is a reservoir of v. cholerae and might be an important source of resistant strains. the aims of this study were to investigate the phylogenetic relationships of intsxt gene sequences from 31 clinical and 14 environmental v. cholerae o1 and non-o1/non-o139 isolates and 11 sequences amplified directly from environmental water samples. we also amplified class 1 integron ... | 2017 | 28412524 |
| cholera returns to southern vietnam in an outbreak associated with consuming unsafe water through iced tea: a matched case-control study. | after more than a decade of steadily declining notifications, the number of reported cholera cases has recently increased in vietnam. we conducted a matched case-control study to investigate transmission of cholera during an outbreak in ben tre, southern vietnam, and to explore the associated risk factors. | 2017 | 28406946 |
| crispr-cas and contact-dependent secretion systems present on excisable pathogenicity islands with conserved recombination modules. | pathogenicity islands (pais) are mobile integrated genetic elements that contain a diverse range of virulence factors. pais integrate into the host chromosome at a trna locus that contains their specific bacterial attachment site, attb, via integrase-mediated site-specific recombination generating attl and attr sites. we identified conserved recombination modules (integrases and att sites) previously described in choleragenic vibrio cholerae pais but with novel cargo genes. clustered regularly i ... | 2017 | 28264992 |
| antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves. | fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ... | 2017 | 28698913 |
| identification and growth optimization of a marine bacillus dk1-sa11 having potential of producing broad spectrum antimicrobial compounds. | control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. marine bacteria are an enormous source of bio-controlling agents. the aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain dk1-sa11 isolated from qingdao bay of china yellow sea. microscopic characterization, api® 20e and 50 chb kit base carbohy ... | 2017 | 28653930 |
| prevalence of enteric infections among hospitalized patients in two referral hospitals in ghana. | diarrhea is an important cause of morbidity and mortality worldwide. in africa and ghana in particular, it is estimated to contribute directly to 19 and 25% of pediatric mortality among children under 5 years, respectively. | 2017 | 28716138 |
| antiprotozoal, antibacterial and antidiarrheal properties from the flowers of chiranthodendron pentadactylon and isolated flavonoids. | chiranthodendron pentadactylon larreat. (sterculiaceae) is a mexican plant used in traditional medicine for the treatment of heart disease symptoms and infectious diarrhea. | 2017 | 28539715 |
| great cormorants (phalacrocorax carbo) as potential vectors for the dispersal of vibrio cholerae. | vibrio cholerae is the cause of cholera, a devastating epidemic and pandemic disease. despite its importance, the way of its global dissemination is unknown. v. cholerae is abundant in aquatic habitats and is known to be borne by copepods, chironomids and fishes. our aim was to determine if fish-eating birds act as vectors in the spread of v. cholerae by consuming infected fish. we determined the existence of v. cholerae in the microbiome of 5/7 wild cormorants' intestine. in three of these v. c ... | 2017 | 28801549 |
| enhancing multiplex genome editing by natural transformation (mugent) via inactivation of ssdna exonucleases. | recently, we described a method for multiplex genome editing by natural transformation (mugent). mutant constructs for mugent require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates laborious in vitro dna splicing. in vibrio cholerae, we uncover that this requirement is due to cytoplasmic ssdna exonucleases, which inhibit natural transformation. in ssdna exonuclease mutants, one arm of homology can be reduced to as little as 40 bp while still promoting integrat ... | 2017 | 28575400 |
| detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization. | simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses. | 2017 | 28713514 |
| development of a novel rationally designed antibiotic to inhibit a nontraditional bacterial target. | the search for new nontraditional targets is a high priority in antibiotic design today. bacterial membrane energetics based on sodium ion circulation offers potential alternative targets. the present work identifies the na(+)-translocating nadh:ubiquinone oxidoreductase (na(+)-nqr), a key respiratory enzyme in many microbial pathogens, as indispensible for the chlamydia trachomatis infectious process. infection by chlamydia trachomatis significantly increased first h(+) and then na(+) levels wi ... | 2017 | 28425301 |
| use of serologic responses against enteropathogens to assess the impact of a point-of-use water filter: a randomized controlled trial in western province, rwanda. | diarrhea is a leading contributor to childhood morbidity and mortality in sub-saharan africa. given the challenge of blinding most water, sanitation, and hygiene (wash) interventions, diarrheal disease outcome measures in wash intervention trials are subject to potential bias and misclassification. using the platform of a cluster-randomized controlled trial of a household-based drinking water filter in western province, rwanda, we assessed the impact of the drinking water filter on enteric seroc ... | 2017 | 28749764 |
| correlation of edge truncation with antibacterial activity of plate-like anisotropic silver nanoparticles. | the effect of silver nanoparticle anisotropy on the antibacterial properties has been studied against escherichia coli, staphylococcus aureus, bacillus, vibrio cholerae, and streptococcus pyogenes. anisotropic silver nanoparticles have been synthesized by solvothermal process. the uv-visible absorption, x-ray diffraction, and tem studies show the anisotropic nature of silver nanoparticles. the results demonstrate that the anisotropic silver nanoparticles undergo a shape-dependent interaction wit ... | 2017 | 28707245 |
| characterization of the escherichia coli concentrative nucleoside transporter nupc using computational, biochemical, and biophysical methods. | members of the concentrative nucleoside transporter (cnt) family of proteins mediate uptake of nucleosides into cells driven by a cation gradient, which then enter salvage pathways for nucleic acid synthesis. in humans, they also transport hydrophilic anticancer and antiviral nucleoside analogue drugs into cells and tissues where they exert their pharmacological effects. escherichia coli cnt nupc (400 residues) is pyrimidine-specific and driven by a proton gradient. we have used computational, b ... | 2017 | 28682624 |
| putative protein vc0395_0300 from vibrio cholerae is a diguanylate cyclase with a role in biofilm formation. | the hallmark of the lifecycle of vibrio cholerae is its ability to switch between two lifestyles - the sessile, non-pathogenic form and the motile, infectious form in human hosts. one of these changes is in the formation of surface biofilms, when in sessile aquatic habitats. the cell-cell interactions within a v. cholerae biofilm are stabilized by the production of an exopolysachharide (eps) matrix, which in turn is regulated by the ubiquitous secondary messenger, cyclic di-gmp (c-di-gmp), synth ... | 2017 | 28647124 |
| electrostatic interactions between the ctx phage minor coat protein and the bacterial host receptor tola drive the pathogenic conversion of vibrio cholerae. | vibrio cholerae is a natural inhabitant of aquatic environments and converts to a pathogen upon infection by a filamentous phage, ctxφ, that transmits the cholera toxin-encoding genes. this toxigenic conversion of v. cholerae has evident implication in both genome plasticity and epidemic risk, but the early stages of the infection have not been thoroughly studied. ctxφ transit across the bacterial periplasm requires binding between the minor coat protein named piii and a bacterial inner-membrane ... | 2017 | 28642371 |
| a versatile toolbox for the control of protein levels using n(ε)-acetyl-l-lysine dependent amber suppression. | the analysis of the function of essential genes in vivo depends on the ability to experimentally modulate levels of their protein products. current methods to address this are based on transcriptional or post-transcriptional regulation of mrnas, but approaches based on the exploitation of translation regulation have so far been neglected. here we describe a toolbox, based on amber suppression in the presence of n(ε)-acetyl-l-lysine (ack), for translational tuning of protein output. we chose the ... | 2017 | 28594177 |
| evaluation of synergy and bacterial regrowth in photocatalytic ozonation disinfection of municipal wastewater. | the use of solar and ultraviolet titanium dioxide photocatalytic ozonation processes to inactivate waterborne pathogens (escherichia coli, salmonella species, shigella species and vibrio cholerae) in synthetic water and secondary municipal wastewater effluent is presented. the performance indicators were bacterial inactivation efficiency, post-disinfection regrowth and synergy effects (collaboration) between ozonation and photocatalysis (photocatalytic ozonation). photocatalytic ozonation effect ... | 2017 | 28577398 |
| histo-blood group antigens as mediators of infections. | the critical first step of a microbial infection is usually the attachment of pathogens to host cell glycans. targets on host tissues are in particular the histo-blood group antigens (hbgas), which are present in rich diversity in the mucus layer and on the underlying mucosa. recent structural and functional studies have revealed significant new insight into the molecular mechanisms, explaining why individuals with certain blood groups are at increased risk of some infections. the most prominent ... | 2017 | 28544984 |
| structural studies of the periplasmic portion of the diguanylate cyclase cdgh from vibrio cholerae. | cyclic diguanylate monophosphate (c-di-gmp) is a second messenger involved in bacterial signal transduction and produced by diguanylate cyclases (dgcs) generally containing highly variable periplasmic signal-recognition domains. cdgh is a dgc enzyme that regulates rugosity associated phenotypes in vibrio cholerae. cdgh has two n-terminal tandem periplasmic substrate-binding (pbpb) domains for its signal recognition; however, the role of the tandem pbpb domains remains unclear. here, we reported ... | 2017 | 28500346 |
| rstb regulates expression of the photobacterium damselae subsp. damselae major virulence factors damselysin, phobalysin p and phobalysin c. | the marine pathogenic bacterium photobacterium damselae subsp. damselae causes septicemia in marine animals and in humans. the pphdd1 plasmid-encoded hemolysins damselysin (dly) and phobalysin p (phlyp), and the chromosome-encoded hemolysin phobalysin c (phlyc) constitute its main virulence factors. however, the mechanisms by which expression of these three hemolysins is regulated remain unknown. here we report the isolation of a mini-tn10 transposon mutant which showed a strong impairment in it ... | 2017 | 28443076 |
| repurposing a two-component system-based biosensor for the killing of vibrio cholerae. | new strategies to control cholera are urgently needed. this study develops an in vitro proof-of-concept sense-and-kill system in a wild-type escherichia coli strain to target the causative pathogen vibrio cholerae using a synthetic biology approach. our engineered e. coli specifically detects v. cholerae via its quorum-sensing molecule cai-1 and responds by expressing the lysis protein yebf-art-085, thereby self-lysing to release the killing protein art-085 to kill v. cholerae. for this report, ... | 2017 | 28441472 |
| a comprehensive system for radiotherapy treatment planning using the pdp-12 computer. | 2016 | 4783119 | |
| [biochemical effects of continuous and discontinuous muscular work (interval work)]. | 2016 | 4720306 | |
| [biochemical effects of continuous and discontinuous muscular work (interval work)]. | 2016 | 4720306 | |
| an intrarenal foreign body. | 2016 | 5041547 | |
| systematic desensitization and expectancy in the treatment of speaking anxiety. | 2016 | 5030248 | |
| regulatory aspects of parenteral drugs. | 2016 | 5012204 | |
| behaviour of carbonic anhydrase in the uterus of hens during moult. | 2016 | 4958241 | |
| nondialyzable urinary hydroxyproline as an index of bone collagen formation. | 2016 | 4983851 | |
| hospital indicators. | 2016 | 4820306 | |
| towards a physical understanding of physiological excitation as a cooperative specific adsorption phenomenon. | 2016 | 4783696 | |
| [evaluation of radical surgery of hirschsprung's disease--so-called suruga's method]. | 2016 | 5052743 | |
| [vascular reconstruction in arteriosclerosis obliterans, with special reference to gas endarterectomy]. | 2016 | 5052741 | |
| chemical methods for removing radon and radon daughters from air. | liquid bromine trifluoride and the solid complexes clf(2)sbf(6), brf(2)sbf(6), brf(4)sb(2)f(11), if(4)(sbf(6))(3) and brf(2)bif(6) react spontaneously with radon and radon daughters at 25 degrees c, converting the radioelements to nonvolatile ions and compounds. the reagents can be used in gas-scrubbing units to remove radon and radon daughters from air. the halogen fluoride-antimony pentafluoride complexes may be suitable for purifying air in uranium mines and analyzing radon in air, since they ... | 2016 | 5013675 |
| information exchange and computerized data retrieval for toxicology. | 2016 | 4852455 | |
| [interaction of dna with alkylating agents of different classes]. | 2016 | 4763787 | |
| [consent to medical and surgical services]. | 2016 | 4742617 | |
| [letter: influence of meteorological conditions on the occurrence of spontaneous pneumothorax]. | 2016 | 4849084 | |
| calcifying primary mucus-producing adenocarcinoma of the gall-bladder. | 2016 | 5029033 | |
| characterization of the factor deficient in the hunter syndrome by polyacrylamide gel electrophoresis. | 2016 | 4987382 | |
| [diagnostic ion-exchange preparations for determination of gastric juice acidity without intubation]. | 2016 | 5035662 | |
| motor conduction velocity studies in patients with leprosy reaction treated with thalidomide and other drugs. | 2016 | 4918201 | |
| st. leonard's hospital, york. | 2016 | 4905662 | |
| chelation therapy in wegener's granulomatosis. treatment with edta. | 2016 | 4975577 | |
| main currents of psychiatric development. reply to the critics. | 2016 | 4873019 | |
| cardiac rehabilitation: a view. | 2016 | 4771366 | |
| comparability of the american cancer society and national institutes of health peer review systems. | 2016 | 4739656 | |
| [work saving studies on methods for making inlays]. | 2016 | 4930215 | |
| charles rouget (1824-1904) successor to claude bernard. | 2016 | 4909258 | |
| carbidopa in parkinson disease and in nausea and vomiting of levodopa. | 2016 | 4834974 | |
| the use of ketamine in psychiatry. | 2016 | 4800188 | |
| halothane in outpatient dental anesthesia. | 2016 | 4863578 | |
| training family therapists by setting learning objectives. | 2016 | 4711087 | |
| [contractile function of the myocardium in patients with the hypertensive form of glomerulonephritis]. | 2016 | 5017214 | |
| viscosity effects on the photohydration of pyrimidines. | 2016 | 5080441 | |
| [use of x-raycinematography of the heart in investigation of children having sustained rheumocarditis]. | 2016 | 5043575 | |
| the gerbich blood group system, especially in melanesians. | 2016 | 5011657 | |
| selenium. | 2016 | 4918952 | |
| mary--a paranoid schizophrenic. | 2016 | 4844741 | |
| [forced vital capacity, one-second capacity and peak-flow rate in 13798 volunteers (author's transl)]. | 2016 | 4809950 | |
| [clinical and morphological study on 2 cases of gaucher's disease in 2 subjects from the same family]. | 2016 | 4804229 | |
| prognostic correlations and response to treatment in advanced metastatic malignant melanoma. | 2016 | 4842252 | |
| [drug therapy in hypertension]. | 2016 | 4766384 | |
| [determination and correction of hyperopia (author's transl)]. | 2016 | 4776075 | |
| [experience with ultraviolet irradiation of the blood in chronic diseases]. | 2016 | 4837149 | |
| self-evaluation by first-year medical students in a clinical science programme. | 2016 | 4771337 | |
| postprandial drowsiness. | 2016 | 5067525 | |
| tissue distribution and development of some cartilage enzymes. | 2016 | 5061875 | |
| effects of legal abortion on gynaecology. | the effect of the abortion act on gynaecological work in one provincial teaching hospital is that despite an increase in patient turnover of 45% the waiting list has increased by 200%. it is suggested that the act has had little effect on the birth rate or illegitimacy and that there may have been an increase in criminal abortion. | 2016 | 5041141 |
| stuttering and social isolation. effect of social isolation with different levels of monitoring on stuttering frequency. (a pilot study). | 2016 | 5035730 | |
| evaluation of the kaufman anti-incontinence procedure: experience with 14 patients. | 2016 | 5081715 | |
| blood distribution through the lung after pneumonectomy: a quantitative assessment by 131-i. | 2016 | 5091637 | |
| structure and biosynthesis of the hydroxy fatty acids of cutin in vicia faba leaves. | 2016 | 5025632 | |
| [prevention of nephrolithiasis]. | 2016 | 5021884 | |
| [therapeutic application of the preparation d 1 s in cardio-vascular neurosis]. | 2016 | 5018516 | |
| can we avoid a 100 billion dollars misunderstanding? | 2016 | 4992557 | |
| [artificial blood specific rh immunization with subsequent pregnancy]. | 2016 | 4989425 | |
| two new myxosporida, henneguya gambusi sp. n. and myxosoma pharyngeus sp. n., in the mosquitofish, gambusia affinis (baird and girard). | 2016 | 5003274 | |
| aspects of the pathology of presenile dementia. | 2016 | 4917682 | |
| [prof. claude verdan's 60th birthday]. | 2016 | 4899435 | |
| resonance energy transfer in pepsin conjugates. | 2016 | 4897805 | |
| gibson wa: histochemical titles from current literature. | 2016 | 4868307 | |
| selection of donor for cardiac transplant. | 2016 | 4866697 | |
| [precursor cells for osteogenic and hemopoietic tissues. analysis of heterotopic transplants of bone marrow]. | 2016 | 4885074 | |
| coexisting addison's disease and diabetes mellitus: report of 24 cases with review of literature. | 2016 | 4888918 | |
| hypersomatotropism and acanthosis nigricans in two brothers. | 2016 | 4843205 | |
| recent advances in anthelmintics. | 2016 | 4862697 |