Publications

identification of glycosylated protein antigens of treponema pallidum and treponema phagedenis.	comparison of autoradiographies of intrinsically [35s] methionine and [14c] glucosamine labeled treponema pallidum (nichols strain) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) revealed four glycosylated proteins with molecular weights 30,500, 33,000, 35,000, and 59,000. t. phagedenis (biotype reiter) was comparatively investigated and showed only two glycosylated proteins with molecular weights 33,000 and 34,000. the at the first time in treponemes identified glyco ...	1985	3901616
sensitivity and specificity of monoclonal antibodies directed against antigenic determinants of treponema pallidum nichols in the diagnosis of syphilis.	murine anti-treponema pallidum monoclonal antibodies were employed in studies on sensitivity and specificity of binding to examine their potential for use in the detection of low numbers of pathogenic treponemes present in various body fluids. monoclonal antibodies were used as a primary antibody source in a solid-phase immunoblot assay system. all monoclonal antibodies assayed were capable of detecting ca. 1.0 x 10(3) to 2.5 x 10(3) treponemes. of 13 monoclonal antibodies examined, 3 were able ...	1984	6208218
monoclonal antibody with hemagglutination, immobilization, and neutralization activities defines an immunodominant, 47,000 mol wt, surface-exposed immunogen of treponema pallidum (nichols).	radioimmunoprecipitation (rip) analyses performed on 125i-surface-labeled treponema pallidum cells using various immune sera revealed the presence of six major surface antigens (immunogens) with apparent molecular weights of 47 k, 36 k, 34 k, 32 k, 29 k, and 13 k. among these, the 47 k surface antigen was most abundant. radioimmunoprecipitation assays using 125i-labeled t. phagedenis biotype reiter or immunoblot analyses using the same strain, failed to reveal the presence of the 47 k mol wt ant ...	1984	6208310
molecular analysis of immunoglobulins m and g immune response to protein antigens of treponema pallidum in human syphilis.	protein antigens of treponema pallidum precipitated by immunoglobulin m (igm) and igg antibodies of sera from patients with untreated primary and secondary syphilis as well as treated secondary syphilis were characterized on a molecular basis. t. pallidum was labeled internally with [35s]methionine and solubilized in 0.1% sodium dodecyl sulfate-1% triton x-100. sodium dodecyl sulfate-polyacrylamide gel electrophoresis on 12.5% gels followed by autoradiography revealed 32 distinct proteins with m ...	1984	6360891
[the enzyme-linked immunosorbent assay technic in syphilis serology (report on experiences)].	a serological study with an enzyme-linked immunosorbent assay using the axial filament of treponema phagedenis biotype reiter as antigen was performed on 1899 sera to evaluate the sensitivity and specificity. the sensitivity as compared with the fluorescent treponemal antibody-absorption test (fta-abs) and the automated microhaemagglutination assay with treponema pallidum antigen ( amha -tp) was 94.6% and 93.1%, respectively. the specificity was 98.5% compared to both assays. furthermore, prelim ...	1984	6375144
antigenic cross-reactivity between treponema pallidum and other pathogenic members of the family spirochaetaceae.	the antigenic cross-reactivity between treponema pallidum and several pathogenic members of the family spirochaetaceae was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting techniques. blots of t. pallidum antigens were incubated with antiserum from rabbits infected or immunized with t. pallidum, treponema paraluiscuniculi, treponema hyodysenteriae (strains b204 and t22), borrelia hermsii serotype 7, or leptopsira interrogans serogroup canicola. t. pallid ...	1984	6384040
molecular characterization of glycoprotein antigens on surface of treponema pallidum: comparison with nonpathogenic treponema phagedenis biotype reiter.	four glycoproteins of treponema pallidum were identified by intrinsic [14c]glucosamine labeling. only two glycoproteins were demonstrated in t. phagedenis biotype reiter with the same technique. glycoproteins of both treponemes were characterized as antigens and shown to be localized within the outer membranes of the microorganisms.	1984	6389370
serological diagnosis of erythema migrans disease and related disorders.	an indirect immunofluorescence technique for the determination of antibodies against ixodid tick spirochetes is described. differences in the reactivity between ixodes ricinus spirochete and ixodes dammini spirochete antigens were not observed. cross-reacting antibodies against treponema pallidum and treponema phagedenis can be eliminated by quantitative absorption with t. phagedenis. cross-reactions with leptospira were not observed by immunofluorescence. in the igm test, false negative reactio ...	1984	6392104
chemotaxis in experimental syphilis.	peritoneal exudate leukocytes from normal and treponema pallidum-infected rabbits were examined for their chemotactic response to various chemoattractants and treponemal preparations. leukocytes from normal and infected animals responded well to bacterial and serum-derived chemoattractants. suspensions of washed treponema phagedenis biotype reiter but not t. pallidum exerted significant chemotactic activity. cells from rabbits infected for greater than or equal to 7 days demonstrated increased l ...	1984	6500721
colonial morphology of treponemes observed by electron microscopy.	the colonial morphology of three strains of cultivable, nonpathogenic treponemes including a human oral treponeme was examined by light and electron microscopy. treponema phagedenis strains kazan and reiter produced large white colonies on the surface of solid media composed of sterility test broth, 0.9 to 3.1% agar, rifampin, and 12.5% rabbit or horse serum. a human oral treponeme, strain g7201 , grew as diffused white zones on 0.9 to 3.1% agar plates. under the cultural conditions employed aga ...	1984	6727709
molecular characterization of common treponemal antigens.	a molecular characterization of cross-reactive antigens of treponema pallidum nichols and treponema phagedenis biotype reiter that are reactive with normal and syphilitic human sera is described. at least 8 common polypeptides, 14 t. pallidum-specific antigens, and 2 t. phagedenis biotype reiter-specific antigens were identified.	1983	6341249
development of monoclonal antibodies that recognize treponema pallidum.	we developed a panel of monoclonal antibodies to treponema pallidum (nichols) antigens, some of which recognize treponemal antigens on t. pallidum (nichols), t. pallidum strain 14, and treponema phagedenis biotype reiter. the antibodies were detected by either an enzyme-linked immunosorbent assay or a radioimmunoassay.	1983	6347899
cloning and expression of treponema pallidum (nichols) antigen genes in escherichia coli.	hybrid pbr322 plasmid clone banks comprised of more than 125,000 recombinant dna clones and representing the entire treponema pallidum nichols genome were constructed in escherichia coli k-12 rr1. the two clone banks individually contain over 53,000 and 72,000 recombinant clones. the number average and mass average sizes of the cloned dna inserts were found to be approximately 12 and 13 kilobase pairs, respectively, indicating the presence of large treponemal dna inserts in a majority of recombi ...	1983	6358023
circulating immune complexes in experimental syphilis: identification of treponemal antigens and specific antibodies to treponemal antigens in isolated complexes.	as a prelude to characterization of the host and treponemal antigens present in purified immune complexes from the sera of rabbits with disseminated syphilis, autoradiographic and immunoenzymatic analyses of solubilized extracts of treponema pallidum, treponema phagedenis biotype reiter, and treponema refringens were performed on electroblots of polypeptides first separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. electroblots of purified immune complexes were developed with ...	1983	6358026
lipid catabolism of cultivated treponemes.	treponema require long-chain fatty acids for growth in vitro. serum, added to culture media, provides a source of long-chain fatty acids. these fatty acids, however, are esterified to triglycerides, phospholipids, and cholesterol. in this study, the major pathways of complex lipid catabolism in t. phagedenis, t. denticola, t. refringens, t. minutum, and t. vincentii were investigated. lipase activity was demonstrated in five treponema species using four lipid substrates. chromatographic data dem ...	1982	6811121
mitogenic responses of hamsters infected with treponema pertenue lack of correlation with passive transfer of resistance.	infection of the cb/ss lak hamster with treponema pertenue is characterised by chronic cutaneous lesions and lymph nodes teeming with treponemes. throughout the course of infection lymph node and spleen cells responded poorly to the mitogens concanavalin a, phytohaemagglutinin, and lipopolysaccharide. this impairment preceded clinical signs of infection and correlated well with the chronicity of framboesial infection. high concentrations of antigen from t pertenue, but not from the non-pathogeni ...	1982	7127055
fumarate reduction and product formation by the reiter strain of treponema phagedenis.	the catabolic pathways for butyrate, acetate, succinate, and ethanol formation by the reiter strain of treponema phagedenis were investigated. enzyme activities were demonstrated for glucose catabolism to pyruvate by the embden-meyerhof-parnas pathway. butyrate formation from acetyl-coenzyme a (acetyl-coa) does not generate atp by substrate level phosphorylation and involves nad+-dependent 3-hydroxybutyryl-coa dehydrogenase and nad(p)+-independent butyryl-coa dehydrogenase activities. butyrate i ...	1982	7142104
pyruvate oxidation by the reiter strain of treponema phagedenis.	spectrophotometric assays of pyruvate oxidation catalyzed by extracts of the reiter strain of treponema phagedenis indicated that viologen dyes, flavin nucleotides, and a ferric iron chelate, but not pyridine nucleotides, were utilized as electron acceptors. benzyl viologen-linked activity partially sedimented during ultracentrifugation and appeared similar to clostridial pyruvate:ferredoxin oxidoreductase with respect to the spectral properties of the enzyme chromophore. electron carrier activi ...	1982	7142105
ribonucleic acid antigen from the reiter treponeme used in an elisa for antibodies in syphilis.	purified rna from treponema phagedenis biotype reiter was used as antigen in an enzyme-linked immunosorbent assay (elisa) for igg antibodies in syphilis. the rna elisa was compared with the fluorescent treponemal antibody absorption test (fta-abs), the treponema pallidum immobilization test (tpi), and with two elisas using purified flagellum from the reiter treponeme as antigen (flagellum elisa), and sonified reiter treponeme culture as antigen (sonicate elisa). a total of 729 sera from patients ...	1982	6184774
parasitism by virulent treponema pallidum of host cell surfaces.	the interaction between virulent treponema pallidum extracted from infected rabbit testes and animal cells in culture was examined. the extent of treponemal attachment to monolayers of normal rabbit testicular and hep-2 cells was dependent upon the incubation temperature and retained motility of the spirochetes. the specific orientation of treponemes to host cell surfaces was demonstrated by dark-field microscopic examination of wet-mount preparations and scanning and transmission electron micro ...	1977	328394
immune responses to soluble antigens of treponemes.	sonicates from five cultivable treponemes were used as antigens in delayed hypersensitivity tests and macrophage inhibition assays. immunodiffusion analysis showed that the sonicate comprised two major antigenic components which were not distinguishable in the skin tests. the sonicate antigens elicited significant cell-mediated immunity in guinea-pigs. treponema refringens biotype nichols proved to induce the strongest delayed response. delayed skin hypersensitivity to the antigens of treponema ...	1977	402976
the effects of glucosamine derivatives on the growth of cultivable treponema.	the effects of glucosamine and its derivatives on the growth of treponema phagedenis biotypes reiter and kazan 5 were studied to determine if these agents could act as growth stimulants for organisms of the genus treptonema. growth studies on the cultivable treponemes were conducted in culture tubes containing glucosamine, glucosamine derivatives, and (or) glucose and galactose in serum-based media. glucosamine and n-phenylacetylglucosamine were definitely inhibitory to growth at the concentrati ...	1976	974903
treponeme outer envelope: chemical analysis.	the chemical composition of the outer envelope (oe) of treponema phagedenis biovar kazan 5 was investigated. after cultivation in a lipid-defined medium, the oe was removed from the cells with 0.7 mm sodium dodecyl sulfate. the solubilized oe was reaggregated by dialysis against 20 mm mgcl2, washed, lyophilized, and subjected to chemical analysis. the average yield of oe was 14.6% of the whole cell (wc) dry weight. the magnesium content was 0.683 mug/mg oe. peptidoglycan components such as muram ...	1976	1250863
the ultrastructure of cultivable treponemes. 1. treponema phagedenis, treponema vincentii and treponema refringens.		1974	4137302
purification and characterization of axial filaments from treponema phagedenis biotype reiterii (the reiter treponeme).	axial filaments have been purified from treponema phagedenis biotype reiterii (the reiter treponeme) and partially characterized chemically. the preparations consist largely of protein but also contain small amounts of hexose (3%). filaments dissociate to subunits in acid, alkali, urea, guanidine, and various detergents. amino acid analyses show an overall resemblance to other spirochetal axial filaments and to bacterial flagella. dissociated filaments migrate as a single band upon acrylamide ge ...	1974	4436261