Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| restricted gene flow in calomys musculinus (rodentia, muridae), the natural reservoir of junin virus. | random amplified polymorphic dna polymerase chain reaction (rapd-pcr) markers were used to evaluate the relative contribution of gene flow as a determinant of the population genetic structure of the wild rodent calomys musculinus (the reservoir of argentine hemorrhagic fever [ahf]) in central argentina. one hundred eighty-seven individuals from 13 populations (9 of them from the endemic zone of ahf and 5 from areas outside it) were analyzed using 78 polymorphic rapd loci. genetic variation withi ... | 2003 | 14691315 |
| junin virus-induced astrocytosis is impaired by inos inhibition. | because junin virus (jv) experimental encephalitis of mice and rats is characterized by mild histopathological changes that do not seem to justify per se lethality after intracerebral infection, such a murine model seems adequate to investigate the potential role of inducible nitric oxide synthase (inos) as a pathogenic factor. concomitant with a predominant astrocyte reaction, increased immunoperoxidase expression of inos, mitochondrial superoxide dismutase (sodm) and glutathione peroxidase (gp ... | 2003 | 12436490 |
| igg subclasses in human immune response to wild and attenuated (vaccine) junin virus infection. | different proportions of igg subclasses have previously been reported to distinguish the immune response elicited by primary and recurrent viral infections, as well as viral vaccines. the goal of this study was to study the igg subclasses composition in the immune response of patients with argentine hemorrhagic fever, and vaccinees with candid #1 strain of junin virus. twenty-four individuals inoculated with candid #1 vaccine and 67 patients with argentine hemorrhagic fever were studied. blood s ... | 2003 | 12526057 |
| arenaviruses other than lassa virus. | the family arenaviridae includes 23 viral species, of which 5 can cause viral hemorrhagic fevers with a case fatality rate of about 20%. these five viruses are junin, machupo, guanarito, sabia and lassa virus, the manipulation of which requires biosafety level 4 facilities. they are included in the category a pathogen list established by the center for disease control and prevention that groups agents with the greatest potential for adverse public health impact and mass casualties whether a situ ... | 2003 | 12615305 |
| differential inhibitory action of two azoic compounds against arenaviruses. | the action of five azo-based compounds against the arenaviruses junin (junv) and tacaribe (tcrv) was evaluated in vitro by a virus yield inhibition assay in vero cells and a cell-free virion inactivation assay. the compound 2-azo-(1'-(2'-nitroso)naphthyl)-benzoate (annb) was the most effective inhibitor of arenavirus production in vero cells with ec(50) (effective concentration 50%) values in the range 6.5-26.2 microm and without inactivating properties. by contrast, the azodicarbonamide (ada) w ... | 2003 | 12672577 |
| inactivation of s-adenosyl-l-homocysteine hydrolase with novel 5'-thioadenosine derivatives. antiviral effects. | synthesis of 5'-s-vinyl-5'-thioadenosine 5, 5'-s-ethynyl-5'-thioadenosine 7 and 5'-s-cyano-5'-thioadenosine 9 is described. incubation of adohcy hydrolase with 5, 7 and 9 resulted in time- and concentration-dependent inactivation of the enzyme and partial depletion of its nad(+) content. from these results and characterisation of metabolites released during the inactivation process, hypothetical mechanisms are suggested. the antiviral activity of 5, 7 and 9 was examined. significant activities w ... | 2003 | 12729633 |
| inhibitory effect of thiosemicarbazone derivatives on junin virus replication in vitro. | the inhibitory effect of several thiosemicarbazones (tscs), synthesized from aromatic ketones and terpenones, and their heterocyclic thiadiazoline (tdz) derivatives, was investigated against junin virus (junv), an arenavirus agent of argentine haemorrhagic fever. from the 25 compounds tested, six compounds belonging to the tsc group were found to be selective inhibitors of junv, with ec50 values determined by a virus yield inhibition assay in the range 3.4-12.5 microm, and selectivity indices gr ... | 2003 | 12856921 |
| endothelial cell function alteration after junin virus infection. | hematologic involvement is the main feature of argentine hemorrhagic fever (ahf), an endemo-epidemic disease caused by junin virus (jv). since endothelial dysfunction could play a role in ahf-altered hemostasis, we studied human umbilical vein endothelial cell (huvec) infection with a virulent (jvv) and a non-virulent (jva) jv strain. cells were infected by the two jv variants with no detectable apoptosis or cytopathic effect. both viral variants up-regulated icam-1 and vcam-1 levels, while von ... | 2003 | 12888881 |
| long-lasting astrocyte reaction to persistent junin virus infection of rat cortical neurons. | immunoperoxidase labeling was performed in histological sections from rat brain harvested during acute (10-30 days), clinically inapparent (90-270 days) and late (450-540 days) stages of junin virus-induced neurological disease. in frontoparietal cortex, count of viral antigen (+) neurons peaked during the acute period (27.7+/-6.8), dropped within the intermediate (4.8+/-4.0 to 1.4+/-1.1) and increased (7.6+/-4.3) at the onset of the late neurological syndrome. in infected vs. control rats, the ... | 2003 | 12898341 |
| polysulfonates derived from metal thiolate complexes as inhibitors of hiv-1 and various other enveloped viruses in vitro. | sodium 2-mercaptoethanesulfonate reacts with the metal ions pd(ii), pt(ii), ag(i), cd(ii) and zn(ii) to yield complexes containing multiple anionic sulfonate sites. on the basis of spectroscopic and other analytical data the complexes were assigned the tentative molecular formulas: pd6(sch2ch2so3na)12, ptn(sch2ch2so3na)2n+2, agn(sch2ch2so3na)n, na2zn4(sch2ch2so3na)10, and na2cd4(sch2ch2so3na)10. the complexes displayed a variety of differences in activity towards dna and rna viruses. the platinu ... | 2002 | 12448691 |
| synthesis and expression of viral antigens in vero cells persistently infected with junin virus. | two vero cell lines persistently infected with xjcl3 and cl67 strains of junin virus and named v3 and v7, respectively, have been characterized with respect to the presence and expression of the nucleoprotein (n) and the glycoprotein precursor (gpc) viral genes. after the acute phase of infection, where a marked cpe and high titers of virus were obtained, jv persistently infected cells became morphologically undistinguishable from vero cells and virus production dropped to undetectable levels. v ... | 2002 | 12181674 |
| mode of inactivation of arenaviruses by disulfide-based compounds. | several disulfide-based compounds, including intermolecular aromatic disulfides of the type ph-s-s-ph and dithianes with the sulfur atoms tethered in a ring structure, have shown effective inhibitory activity against the arenaviruses junin (junv), agent of argentine hemorrhagic fever, and tacaribe (tcrv). these compounds showed a strong virucidal effect with inactivating concentration 50% (ic(50)) values in the range 0.6-5.0 microm, and also were effective to reduce virus yields from infected ce ... | 2002 | 12206881 |
| evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to junin virus in rodents. | junin virus is the etiological agent of argentine hemorrhagic fever, a serious rodent-borne disease. an enzyme-linked immunosorbent assay (elisa) to detect junin virus igg antibodies in rodents was evaluated using sera from 27 calomys musculinus and five calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. the test performance was compared against an indirect immunofluorescence assay (ifa). the elisa had a sensitivity and specificity of 100% and a reproduci ... | 2002 | 11906733 |
| evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to junin virus in rodents. | junin virus is the etiological agent of argentine hemorrhagic fever, a serious rodent-borne disease. an enzyme-linked immunosorbent assay (elisa) to detect junin virus igg antibodies in rodents was evaluated using sera from 27 calomys musculinus and five calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. the test performance was compared against an indirect immunofluorescence assay (ifa). the elisa had a sensitivity and specificity of 100% and a reproduci ... | 2002 | 11906733 |
| evaluation of the antiviral activity against junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of baccharis coridifolia. | four macrocyclic trichothecenes, roridin a, roridin e, verrucarin a and verrucarin j, produced by the hypocrealean epibiont of baccharis coridifolia, were evaluated for their inhibitory activity against the arenavirus junin (junv), the etiological agent of argentine hemorrhagic fever. the trichothecenes achieved a dose-dependent inhibition of junv multiplication at concentrations not affecting cell viability. the 50 % inhibitory concentration (ic50) values determined by a virus yield inhibition ... | 2002 | 11914955 |
| junin virus vaccines. | 2002 | 11987817 | |
| gene flow among calomys musculinus (rodentia, muridae) populations in argentina. | calomys musculinus is a sigmodontinae rodent inhabiting periodically disturbed habitats in the central eastern plains of argentina. it is the natural reservoir host of junin virus, the etiological agent of argentine hemorrhagic fever (ahf). in order to analyze the levels of gene flow among populations of this species, allozymic variability at 26 loci was studied in 291 individuals from the endemic zone of ahf and localities outside it. all populations showed high levels of polymorphism (he betwe ... | 2002 | 11990761 |
| gene flow among calomys musculinus (rodentia, muridae) populations in argentina. | calomys musculinus is a sigmodontinae rodent inhabiting periodically disturbed habitats in the central eastern plains of argentina. it is the natural reservoir host of junin virus, the etiological agent of argentine hemorrhagic fever (ahf). in order to analyze the levels of gene flow among populations of this species, allozymic variability at 26 loci was studied in 291 individuals from the endemic zone of ahf and localities outside it. all populations showed high levels of polymorphism (he betwe ... | 2002 | 11990761 |
| inheritance of random amplified polymorphic dna (rapd-pcr) markers and their use in population genetic studies of calomys musculinus (rodentia, muridae), the reservoir of argentine hemorrhagic fever. | in order to assess the reliability of rapd markers in the estimation of the genetic structure of natural populations of the murid rodent calomys musculinus (reservoir of junin virus, ethiological agent of argentine hemorrhagic fever), we have analyzed the heritability of rapd bands in 10 parents and their offspring (33 individuals). fourteen out of a total of 119 bands obtained were absent in the parental patterns, but consistently amplified in offspring from some families. these bands can be el ... | 2001 | 12035619 |
| inheritance of random amplified polymorphic dna (rapd-pcr) markers and their use in population genetic studies of calomys musculinus (rodentia, muridae), the reservoir of argentine hemorrhagic fever. | in order to assess the reliability of rapd markers in the estimation of the genetic structure of natural populations of the murid rodent calomys musculinus (reservoir of junin virus, ethiological agent of argentine hemorrhagic fever), we have analyzed the heritability of rapd bands in 10 parents and their offspring (33 individuals). fourteen out of a total of 119 bands obtained were absent in the parental patterns, but consistently amplified in offspring from some families. these bands can be el ... | 2001 | 12035619 |
| zinc-binding properties of junín virus nucleocapsid protein. | the arenavirus nucleocapsid protein (n) is a highly basic 63 kda protein with a dual function during the virus life-cycle. first, it is involved in essential steps of genome replication, promoting the synthesis of the full-length antigenomic copy of s rna, and second it associates with the genomic rna to form the nucleocapsid. we have expressed the n protein of junín virus in e. coli and shown that it binds zinc in vitro. this property is in agreement with the presence in the carboxy-terminal re ... | 2001 | 11125165 |
| arenavirus nucleocapsid protein displays a transcriptional antitermination activity in vivo. | rna polymerase pausing and transcriptional antitermination regulates gene activity in several systems. in arenavirus infected cells the switch from transcription to replication is subjected to a hairpin-dependent termination and requires protein synthesis to bypass this signal. the transcriptional antitermination control by junín virus nucleocapsid protein n, has been demonstrated in vivo by infecting bhk-21 cells expressing this viral protein in the presence of translation inhibitors. this is t ... | 2001 | 11163643 |
| involvement of vacuolar proton atpase in junin virus multiplication. | the role of vacuolar-proton atpase (v-h+ atpase) on junin virus (jv) replication was evaluated by analyzing the effect of specific inhibitors of the enzyme activity on different steps of virus multiplication cycle. the presence of the macrolide antibiotics bafilomycin a1 and concanamycin a during the first two hours of infection caused a significant reduction of extracellular infectious virus production and viral protein expression in vero and bhk-21 cells. the inhibitory action of the compounds ... | 2001 | 11315636 |
| effect of fatty acids on arenavirus replication: inhibition of virus production by lauric acid. | to study the functional involvement of cellular membrane properties on arenavirus infection, saturated fatty acids of variable chain length (c10-c18) were evaluated for their inhibitory activity against the multiplication of junin virus (junv). the most active inhibitor was lauric acid (c12), which reduced virus yields of several attenuated and pathogenic strains of junv in a dose dependent manner, without affecting cell viability. fatty acids with shorter or longer chain length had a reduced or ... | 2001 | 11402863 |
| [neutralization test for lymphocytic choriomeningitis virus for distinguishing between two arenavirus infections in argentina]. | the active coexistence of two pathogenic arenaviruses, junin (junv) and lymphocytic choriomeningitis (lcmv), in the same region of argentina, has been known since the early 70's, and records of clinical and subclinical human infections by one and/or the other agent have been continuously produced for the last 25 years. anti-lcmv antibody is currently searched only by indirect immunofluorescence, a test that shows cross reactions among a number of arenaviruses yielding, in the cases of lcmv and j ... | 2001 | 11833256 |
| antiviral effect of brassinosteroids against herpes virus and arenaviruses. | a natural brassinosteroid and a series of synthetic derivatives were found to be good inhibitors of herpes simplex virus type 1 (hsv-1) and arenavirus replication in cell culture. the synthetic compounds tested were analogues of the 24(s) ethylbrassinone. compounds (22 r,23 r,24s)-2alpha, 3alpha,5alpha,22,23-pentahydroxy-stigmastan-6-one and (22r,23r,24s)-3beta-bromo-5alpha,22,23-trihydroxy stigmastan-6-one were cytotoxic at concentrations of 20-40 microm. (22s,23s,24s)-2alpha,3alpha,22,23-tetra ... | 2000 | 10693656 |
| homologous and heterologous glycoproteins induce protection against junin virus challenge in guinea pigs. | tacaribe virus (tacv) is an arenavirus that is genetically and antigenically closely related to junin virus (junv), the aetiological agent of argentine haemorrhagic fever (ahf). it is well established that tacv protects experimental animals fully against an otherwise lethal challenge with junv. to gain information on the nature of the antigens involved in cross-protection, recombinant vaccinia viruses were constructed that express the glycoprotein precursor (vv-gtac) or the nucleocapsid protein ... | 2000 | 10769070 |
| homologous and heterologous glycoproteins induce protection against junin virus challenge in guinea pigs. | tacaribe virus (tacv) is an arenavirus that is genetically and antigenically closely related to junin virus (junv), the aetiological agent of argentine haemorrhagic fever (ahf). it is well established that tacv protects experimental animals fully against an otherwise lethal challenge with junv. to gain information on the nature of the antigens involved in cross-protection, recombinant vaccinia viruses were constructed that express the glycoprotein precursor (vv-gtac) or the nucleocapsid protein ... | 2000 | 10769070 |
| relationship between hematopoietic growth factors levels and hematological parameters in argentine hemorrhagic fever. | argentine hemorrhagic fever (ahf) is a viral disease caused by junin virus and characterized by hematologic and neurological involvement. the main hematologic features are leukopenia, thrombocytopenia, and bone marrow hypoplasia. hematopoietic growth factors serum levels were measured by elisa technique in forty-eight patients with confirmed diagnosis of ahf. patients were classified according to the clinical picture in 15 severe (scf), 17 moderate (mocf), and 16 mild (micf) cases. erythropoieti ... | 2000 | 10815780 |
| genetic diversity of the junin virus in argentina: geographic and temporal patterns. | rna was purified from 39 strains of cell-cultured junin virus (jun) from central argentina, which included both human- and rodent-derived isolates (a total of 26 and 13, respectively), as well as from 2 laboratory jun strains, xj cl3 and xj #44. jun-specific primers were used to amplify a 511-nucleotide (nt) fragment of the nucleocapsid protein gene and a 495-nt fragment of the glycoprotein 1 (gp1) gene. genetic diversity among jun strains studied was up to 13% at the nt level and up to 9% at th ... | 2000 | 10873755 |
| genetic diversity of the junin virus in argentina: geographic and temporal patterns. | rna was purified from 39 strains of cell-cultured junin virus (jun) from central argentina, which included both human- and rodent-derived isolates (a total of 26 and 13, respectively), as well as from 2 laboratory jun strains, xj cl3 and xj #44. jun-specific primers were used to amplify a 511-nucleotide (nt) fragment of the nucleocapsid protein gene and a 495-nt fragment of the glycoprotein 1 (gp1) gene. genetic diversity among jun strains studied was up to 13% at the nt level and up to 9% at th ... | 2000 | 10873755 |
| antiviral and virucidal activities against arenaviruses of zinc-finger active compounds. | fifteen antiretroviral zn-finger active compounds with diverse chemical structures, including azoic compounds, hydrazide derivatives, disulphide-based reagents and others were screened in vitro against junin virus (junv), the aetiological agent of argentine haemorrhagic fever, by a virus yield inhibition assay in vero cells. cytotoxicity was evaluated simultaneously by the mtt method. of the compounds, three were totally inactive as antivirals, nine presented moderate anti junv-activity and thre ... | 2000 | 10901294 |
| a quantitative approach to correlate astrocyte differentiation and phagocytic activity. | a triple staining procedure (pap labeling for gfap, pas reaction for added yeast cells and hematoxylin for nuclear staining of the whole cell monolayer) had disclosed that junin virus infection enhanced phagocytic activity by inducing greater astrocyte differentiation. here, we resorted to a mathematical approach for simultaneous evaluation of astrocyte differentiation and potential phagocytosis. at light microscopy level, the total number of: a) pas-stained yeast cells, b) pas-stained yeast cel ... | 2000 | 10979613 |
| involvement of the cytoskeleton in junin virus multiplication. | the role of the cellular cytoskeleton in junin virus (jv) infection was explored in two ways. firstly, the action of inhibitors that affect individual cytoskeletal systems (microtubules or microfilaments) selectively was analysed. it was found that perturbations of microtubule or microfilament networks caused by colchicine, nocodazole, nifedipine, egta or dmso strongly affected virion production and viral protein expression at non-cytotoxic concentrations. secondly, the extent of association of ... | 1999 | 9934697 |
| proinflammatory cytokines and elastase-alpha-1-antitrypsin in argentine hemorrhagic fever. | argentine hemorrhagic fever (ahf) is a disease caused by junin virus. in the acute phase, patients present hematologic and neurologic involvement with high levels of interferon-alpha and tumor necrosis factor-alpha (tnf-alpha. nineteen patients with a confirmed diagnosis of ahf were studied: six severe, four moderate and nine mild cases. serum levels of interleukin-6 (il-6), il-6 soluble receptor (il-6sr), il-8, il-10, and elastase-alpha1-antitrypsin complex (e-alpha 1at) were assayed by elisas. ... | 1999 | 9988328 |
| evaluation of the antiviral activity of natural sulfated polyhydroxysteroids and their synthetic derivatives and analogs. | disodium 3beta,21-dihydroxypregn-5-en-20-one disulfate (2), sodium 3beta,21-dihydroxypregn-5-en-20-one 3-sulfate (3), sodium 3beta,21-dihydroxypregn-5-en-20-one 21-sulfate (4), and disodium 3beta,6alpha-dihydroxy-5alpha-pregnan-20-one disulfate (6) have been synthesized and completely characterized for the first time from readily available materials. sulfation was performed using triethylamine-sulfur trioxide complex in dimethylformamide as the sulfating agent. selective sulfation of 3beta,21-di ... | 1999 | 10406483 |
| cell differentiation increases astrocyte phagocytic activity. a quantitative analysis of both gfap labeling and pas-stained yeast cells. | since efficiency of phagocytic potential in activated astrocytes is still a subject of controversy, an attempt was made to quantify simultaneously phagocytic activity and astrocyte differentiation. resorting to junin virus, known to induce astrocyte activation, infected vs control samples of cultured rat astroglial cells were serially harvested up to day 12 post-inoculation (pi), and subjected to a triple staining procedure consisting in immunoperoxidase labeling of gfap, periodic acid-schiff (p ... | 1999 | 10413896 |
| clinical case definitions for argentine hemorrhagic fever. | argentine hemorrhagic fever (ahf) is a potentially lethal infection in argentina. the case-fatality ratio is >15%, but treatment reduces the mortality rate to <1%. diagnosis is based on clinical and laboratory criteria, but no case definition has been validated. a chart review was conducted for patients hospitalized with suspected ahf. individuals with a fourfold rise in antibody titer were classified as cases. the combination of a platelet count of <100,000/mm3 and a white blood cell (wbc) coun ... | 1999 | 10452640 |
| analysis of viral glycoproteins by glycosidic digestion inside a polyacrylamide gel. | we adapted the method described by cleveland et al. (1977); (peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. j. biol. chem. 252, 1102-1106) to study the glycosidic residues linked to the viral glycoproteins of two enveloped viruses: junin virus (jv) and rubella virus (rv). radioiodinated glycoproteins were obtained from purified virions, isolated from sds-polyacrylamide gels and then hydrolysed by specific glycosidases inside a second gel. n ... | 1999 | 10471032 |
| [inhibition of junin virus multiplication by caffeine]. | the inhibitory activity of caffeine, a pharmacologically active compound, on junin virus (jv) multiplication in vero cells was evaluated by a virus yield inhibition assay. the compound achieved a dose-dependent inhibition of virus production at concentrations not affecting cell viability. the 50% inhibitory concentration (ic50) and the 90% inhibitory concentration (ic90) were 9.0 mm and 10.8 mm, respectively. from time of addition experiments, it can be concluded that caffeine inhibited an early ... | 1999 | 10509392 |
| myristic acid analogs are inhibitors of junin virus replication. | the effects of two myristic acid analogs on junin virus (jv) replication were investigated. the compounds chosen for the study were dl-2-hydroxymyristic acid (2ohm), an inhibitor of n-myristoyltransferase (nmt), which binds the enzyme and blocks protein myristoylation, and 13-oxamyristic acid (13om), a competitive inhibitor of nmt which incorporates into the protein instead of myristic acid. both types of analogs achieved dose-dependent inhibition of viral multiplication at concentrations not af ... | 1999 | 10611737 |
| protective efficacy of a live attenuated vaccine against argentine hemorrhagic fever. ahf study group. | argentine hemorrhagic fever (ahf), caused by the arenavirus junin, is a major public health problem among agricultural workers in argentina. a prospective, randomized, double-blind, placebo-controlled, efficacy trial of candid 1, a live attenuated junin virus vaccine, was conducted over two consecutive epidemic seasons among 6500 male agricultural workers in the ahf-endemic region. twenty-three men developed laboratory-confirmed ahf during the study; 22 received placebo and 1 received vaccine (v ... | 1998 | 9466512 |
| in vitro anti-junin virus activity of a peptide isolated from melia azedarach l. leaves. | meliacine, a peptide isolated from leaves of melia azedarach l. inhibited the multiplication of junin virus in vero cells treated with the compound before infection (pre-treatment) or immediately after virus adsorption. analysis of early events following infection demonstrated that meliacine blocks virus penetration by preventing the uncoating step. the addition of meliacine at different times after infection indicated that meliacine also interferes with the release of infectious particles to th ... | 1998 | 9624546 |
| effects of reducing, oxidizing and alkylating agents on early steps of junin virus multiplication. | the action of reducing, oxidizing and thiol-alkylating agents on early steps of junin virus (jv) multiplication in vero cells was investigated. the presence of reducing agents during virus adsorption as well as incubation of viral particles with these compounds before infection enhanced jv infectivity. on the contrary, the thiol-alkylating agent 5,5' dithiobis (2-nitrobenzoic acid) and the oxidizing compound potassium periodate showed an inhibitory effect, suggesting that sulfhydryl groups, and ... | 1998 | 9722885 |
| [prevalence of hepatitis c antibodies in plasma donors for the treatment of argentine hemorrhagic fever]. | for argentine hemorrhagic fever, a disease caused by junin virus (jv), there is an effective treatment, consisting of the transfusion of immune plasma (ip). this plasma is obtained from individuals who have had the disease. since hepatitis c virus (hcv) is transmitted parenterally, this study was aimed to estimate the prevalence of anti-hcv in a population of ip donors. in this study, 376 donors (47 females and 329 males) were studied: 95 individuals (24 females and 71 males) who had had fha but ... | 1997 | 9640761 |
| effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of junin virus. | the effect of glycoprotein processing and transport on junin virus (jv) maturation was studied by using brefeldin a (bfa) and carbonyl cyanide m-chlorophenylhydrazone (cccp), two inhibitors affecting different steps of the intracellular exocytic pathway, combined with low temperature incubation. both compounds inhibited the multiplication of jv, strain iv4454, in vero cells in a dose dependent manner. the addition of the compounds after several cycles of infection for a very short treatment peri ... | 1997 | 9672585 |
| evaluation of an enzyme immunosorbent assay for the diagnosis of argentine haemorrhagic fever. | to elaborate a set of serological tests for the diagnosis of argentine haemorrhagic fever (ahf), an enzyme-linked immunosorbent assay (elisa) for detection of specific anti-junin virus (jv) igg is described, and its performance is compared with that of the plaque reduction neutralization test (prnt). the reproducibility, sensitivity, specificity, and confidence limits for positive and negative results for elisa were statistically analysed. the value of 800 was demonstrated as the lowest positive ... | 1997 | 9607087 |
| molecular characteristics of junin virus. | results suggest that protein, glycerophospolipid, galactoside, and sialyl glycoside residues are present in junin virus (jv), are accessible to enzymatic digestion, and play an important role in infection. four major protein bands with molecular masses (mr) 64 +/- 2, 56 +/- 2, 52 +/- 3 (mean +/- standard deviation, n = 4) and approximately 12-18 kda were consistently detected after denaturing gel electrophoresis analysis of purified attenuated jv. the 52 kda protein showed a diffuse tail in the ... | 1997 | 9015273 |
| the glycoprotein precursor gene of the attenuated junin virus vaccine strain (candid #1). | a live attenuated virus vaccine has been recently developed to prevent argentine hemorrhagic fever. in this paper, we report the nucleotide sequence of the glycoprotein precursor gene (gpc) of the junin virus vaccine strain (candid #1) and its flanking untranslated regions. the untranslated regions flanking the gpc genes of different arenaviruses are variable in length, sequence, and secondary structure. however, when this highly attenuated junin virus strain is compared with the mc2 strain, whi ... | 1997 | 9080883 |
| molecular characterization of attenuated junin virus strains. | the junin virus strain candid #1 was developed as a live attenuated vaccine for argentine haemorrhagic fever. in this paper we report the nucleotide sequences of s rna of candid #1 and its more virulent ancestors xj#44 and xj (prototype). their relationship to junin virus wild-type mc2 strain and other closely and distantly related arenaviruses was also examined. comparisons of the nucleotide and amino acid sequences of n and gpc genes from candid #1 and its progenitor strains revealed some chan ... | 1997 | 9225036 |
| glycoprotein-mediated biological properties of a host range mutant of junin virus. | the biological properties mediated by the main envelope glycoprotein (gp1) of a mouse-attenuated mutant of junin virus, named cl67, were investigated in comparison with its parental strain xjcl3. in contrast to the parental strain, this mutant was unable to multiply in primary cultures of mouse embryo fibroblasts (mefs). impairment of cl67 multiplication was associated with a lack of virus binding to mef, probably due to an altered interaction between gp1 and cellular receptors. antigenic and im ... | 1997 | 9403931 |
| medical microbiology | the hallmark of arenaviruses is their tendency to cause persistent silent infections in their natural hosts (rodents) and severe, often lethal, disseminated disease in humans. arenaviruses are pleomorphic enveloped particles that contain two rna segments of virus origin and ribosome-like components. suitable conditions for transmission of virus to humans occur in areas where humans come in contact with rodent urine that contains virus. persistent viremia and viruria in rodents result from a slow ... | 1996 | 21413313 |
| [retrospective detection of hantavirus clinical infections in argentina]. | hantavirus activity in rodents and human beings in argentina has been known since the 1980's. in this study, we retrospectively investigated hantavirus infections among argentine hemorrhagic fever (ahf) cases notified between 1987 and 1994, without virological confirmation. igg and igm antibodies to hantavirus were tested by elisa. among 1028 patients included in the study, we found 13 recent infections (1.26%) and 13 remote infections (1.26%). igg antibodies determined in 745 healthy persons li ... | 1996 | 8734923 |
| low-ph-induced fusion of vero cells infected with junin virus. | junin virus (jv) infected vero cells were used to investigate virus capacity to induce cell-cell fusion. polykaryocyte formation due to jv was found to be ph and temperature-dependent. a reduced fusion activity was detected on bhk-21 cells. different jv-strains exhibited a similar extent and ph dependence of their fusion activity. neutralizing antibodies against the main viral glycoprotein (gp38) inhibited syncytium production and gp38 conformational changes in response to acid treatment were de ... | 1996 | 8774689 |
| antiviral sulfated steroids from the ophiuroid ophioplocus januarii. | one new and three known sulfated steroidal polyols have been isolated from the ophiuroid ophioplocus januarii, collected at san antonio oeste, río negro, argentina. the four compounds possess 4 alpha,11 beta-dihydroxy-3 alpha,21-disulfoxy substituents and the a/b cis ring junction but differ in the side chain. the new compound has been characterized as (22e)-5 beta-24-norcholest-22-ene-3 alpha,4 alpha,11 beta,21-tetrol 3,21-disulfate (4). the structures of the four compounds were determined from ... | 1996 | 8864241 |
| the role of mononuclear blood cells in experimental junin virus spread to the central nervous system. | the neuroinvasiveness of junin virus depends on the viral strain, animal species, and age. the role of infected blood cells in hematogenous junin virus spread to the central nervous system (cns) was studied by determining the growth in pheripheral mononuclear cells and brain tissue of candid 1 and xjcl3 laboratory strains, in calomys musculinus and guinea pigs. the present study demonstrated that junin virus replicates in circulating peripheral lymphocytes and macrophages of 11-day-old guinea pi ... | 1995 | 8825295 |
| the role of mononuclear blood cells in experimental junin virus spread to the central nervous system. | the neuroinvasiveness of junin virus depends on the viral strain, animal species, and age. the role of infected blood cells in hematogenous junin virus spread to the central nervous system (cns) was studied by determining the growth in pheripheral mononuclear cells and brain tissue of candid 1 and xjcl3 laboratory strains, in calomys musculinus and guinea pigs. the present study demonstrated that junin virus replicates in circulating peripheral lymphocytes and macrophages of 11-day-old guinea pi ... | 1995 | 8825295 |
| rapid diagnosis of argentine hemorrhagic fever by reverse transcriptase pcr-based assay. | argentine hemorrhagic fever (ahf) is an endemo-epidemic disease caused by junín virus. this report demonstrates that a reverse transcriptase (rt) pcr-based assay developed in our laboratory to detect junín virus in whole blood samples is sensitive and specific. the experiments were conducted in a double-blinded manner using 94 clinical samples collected in the area in which ahf is endemic. the rt-pcr-based assay was compared with traditional methodologies, including enzyme-linked immunosorbent a ... | 1995 | 7542268 |
| [influence] of enzymatic treatment on junin virus--vero cells interaction]. | the chemical nature of cellular structures involved in the attachment of junin virus (wild type xjc13 and host range mutant cl 67) to vero cells was investigated. enzyme treatment of cells before virus infection indicated that whereas lipids are not directly involved in virus attachment, cellular proteins play a significant role in early interaction with jv. moreover aromatic residues, leucine and basic amino acid seem to actively participate in this interaction with different affinity for the a ... | 1995 | 7568866 |
| early markers of blood coagulation and fibrinolysis activation in argentine hemorrhagic fever. | junin virus, an arenaviridae, is the etiological agent of argentine hemorrhagic fever. in addition to thrombocytopenia, patients present several alterations in both the blood coagulation and the fibrinolytic system, but diffuse intravascular coagulation could not be demonstrated. to investigate further the activation status of the two systems, levels of thrombin-antithrombin complexes (tat), prothrombin fragment 1 + 2, protein c, total and free protein s, c4bbp, antithrombin iii, t-pa, pai-1 and ... | 1995 | 7667817 |
| cosalane analogues with enhanced potencies as inhibitors of hiv-1 protease and integrase. | several new analogues of the novel anti-hiv agent cosalane have been synthesized and evaluated as inhibitors of hiv-1 integrase and protease, hiv-1 replication, hiv-1 and hiv-2 cytopathicity, hiv-1- and hiv-2-mediated syncytium formation, and cytopathicity of a variety of human pathogenic viruses. the congeners displayed enhanced potencies relative to cosalane itself as inhibitors of hiv-1 integrase and protease. the two most potent analogues against hiv-1 integrase displayed ic50 values of 2.2 ... | 1995 | 7853337 |
| glial fibrillary acidic protein (gfap) immunochemical profile after junin virus infection of rat cultured astrocytes. | cultured astrocytes derived from newborn rat brain were inoculated with junin virus (jv) to characterize their response to infection by means of their glial fibrillary acidic protein (gfap) immunochemical profile. samples from 1 to 11 days post-inoculation (pi), as well as matched controls, were serially harvested for gfap labeling by peroxidase-antiperoxidase (pap) method. it was only at day 3 that significantly greater values of gfap staining (p < 0.05) were disclosed by three complementary ap ... | 1995 | 9064605 |
| intracellular processing and transport of junin virus glycoproteins influences virion infectivity. | the influence of glycoprotein processing, cleavage and transport on junin virus (jv) infectivity was investigated using monensin combined with lectin binding assays. yields of extracellular virus were more significantly reduced than cell-associated virus, indicating that monensin inhibited the transport of infectious virus to the extracellular space on a late stage of the replicative cycle. shown by lectin reactivity and immunoprecipitation, the intracellular processing of jv glycoproteins invol ... | 1994 | 7856317 |
| prevalence of infection with junin virus in rodent populations in the epidemic area of argentine hemorrhagic fever. | we report the results of indirect fluorescent antibody screening for antibody to junin virus in 1,101 sera from small mammals captured on two mark-recapture grids in the epidemic area of argentine hemorrhagic fever. twenty-six of 29 seropositive animals were the cricetid rodent calomys musculinus, for a 30-month prevalence of 7.9% in that species. combining these data with previously published data on antigen detection provided an estimated total prevalence of infection of 10.9% for this, the pr ... | 1994 | 7985747 |
| prevalence of infection with junin virus in rodent populations in the epidemic area of argentine hemorrhagic fever. | we report the results of indirect fluorescent antibody screening for antibody to junin virus in 1,101 sera from small mammals captured on two mark-recapture grids in the epidemic area of argentine hemorrhagic fever. twenty-six of 29 seropositive animals were the cricetid rodent calomys musculinus, for a 30-month prevalence of 7.9% in that species. combining these data with previously published data on antigen detection provided an estimated total prevalence of infection of 10.9% for this, the pr ... | 1994 | 7985747 |
| latin american hemorrhagic fever. | several viral and bacterial agents are responsible for hemorrhagic fever in latin america, but there are three agents that are only present in this region: (1) the junin virus, (2) the machupo virus, and (3) the guanaritovirus, members of the tacaribe complex of the family arenaviridae and responsible for the argentinian, bolivian, and venezuelan hemorrhagic fever. the clinical manifestations of hemorrhagic fever are similar for the three viruses, mainly myalgia, arthralgia, headache, relative b ... | 1994 | 8021448 |
| the entry of junin virus into vero cells. | the entry mechanism of junin virus (jv) into vero cells was studied analyzing the effect of lysosomotropic compounds and acid ph on jv infection. ammonium chloride, amantadine, chlorpheniramine and procaine inhibited jv production. the action of ammonium chloride was exerted at early times of infection. virus internalization was inhibited and viral protein expression was not detected. when the extracellular medium was buffered at low ph, the ammonium chloride induced block on jv infection was ov ... | 1994 | 8031239 |
| neural spread of junin virus in intraperitoneally inoculated rats. | on the basis of an already demonstrated junín virus (jv) neural route after peripheral footpad infection of newborn rats, here we attempted to determine the viral pathway following intraperitoneal inoculation. as from the 2nd week post-infection, neurological disease developed reaching 84% mortality at 30 days. immunoperoxidase labeling of viral antigen, concomitantly with infectivity assays and histological examination, was carried out in serially harvested samples. whenever infectivity was det ... | 1994 | 7715431 |
| calomys musculinus, the natural reservoir of argentine haemorrhagic fever, is a semipermissive host for a mouse-attenuated mutant of junin virus. | c167, a mouse-attenuated strain of junin virus derived from the xjc13 strain, also displayed reduced virulence for the south american cricetid calomys musculinus, a natural reservoir of this virus in nature. intracerebral inoculation of c. musculinus with 500 pfu of c167 produced only 25% mortality, whereas the parental xjc13 killed 85% of the animals. the attenuation of c167 for this cricetid was lower than for albino mice. the multiplication of c167 in c. musculinus-derived embryo or kidney fi ... | 1994 | 7839011 |
| calomys musculinus, the natural reservoir of argentine haemorrhagic fever, is a semipermissive host for a mouse-attenuated mutant of junin virus. | c167, a mouse-attenuated strain of junin virus derived from the xjc13 strain, also displayed reduced virulence for the south american cricetid calomys musculinus, a natural reservoir of this virus in nature. intracerebral inoculation of c. musculinus with 500 pfu of c167 produced only 25% mortality, whereas the parental xjc13 killed 85% of the animals. the attenuation of c167 for this cricetid was lower than for albino mice. the multiplication of c167 in c. musculinus-derived embryo or kidney fi ... | 1994 | 7839011 |
| antiviral treatment of argentine hemorrhagic fever. | argentine hemorrhagic fever is a systemic viral disease caused by junin virus, with a mortality of 15-30% in untreated individuals. current specific therapy is highly effective in reducing mortality, and consists of the early administration of immune plasma in defined doses of specific neutralizing antibodies per kg of body weight. however, several reasons suggest the need to investigate alternative therapies. ribavirin, a broad spectrum antiviral agent, is effective in the treatment of other vi ... | 1994 | 8141590 |
| in vitro selection of junin virus antigenic variants. | the establishment of an experimental persistent infection with junin virus, the aetiological agent of argentine hemorrhagic fever, involves the emergence of antigenic variants in brain and blood of the cricetid calomys musculinus. we demonstrate that antigenic variants can also be isolated in vitro under the selective pressure of polyclonal antibodies and from a long-term infected c. musculinus primary embryo fibroblast culture. the participation of neutralizing antibodies and host cells in the ... | 1993 | 8382041 |
| in vitro selection of junin virus antigenic variants. | the establishment of an experimental persistent infection with junin virus, the aetiological agent of argentine hemorrhagic fever, involves the emergence of antigenic variants in brain and blood of the cricetid calomys musculinus. we demonstrate that antigenic variants can also be isolated in vitro under the selective pressure of polyclonal antibodies and from a long-term infected c. musculinus primary embryo fibroblast culture. the participation of neutralizing antibodies and host cells in the ... | 1993 | 8382041 |
| a simple nucleic acid amplification assay for the rapid detection of junín virus in whole blood samples. | argentine hemorrhagic fever (ahf) is an endemoepidemic disease with cardiovascular, renal and neurologic alterations acquired in the richest farming land in argentina. it is caused by junín virus, one of the few human pathogenic arenaviruses. the s rna of junín virus has been molecularly cloned and its nucleotide sequence determined in our laboratory. this information was used to develop a rapid nucleic acid-based diagnostic test commensurate with the low viraemia detected in ahf patients. junín ... | 1993 | 8383393 |
| safety and immunogenicity of a live-attenuated junin (argentine hemorrhagic fever) vaccine in rhesus macaques. | the safety and immunogenicity of candid #1, a live-attenuated junin virus vaccine, were evaluated in rhesus macaques. candid #1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units (pfu) into four groups of five animals each; four controls received saline. there was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. junin virus was recovered by cocultivation from peripheral blood mononuclear cells (p ... | 1993 | 8385886 |
| safety and immunogenicity of a live-attenuated junin (argentine hemorrhagic fever) vaccine in rhesus macaques. | the safety and immunogenicity of candid #1, a live-attenuated junin virus vaccine, were evaluated in rhesus macaques. candid #1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units (pfu) into four groups of five animals each; four controls received saline. there was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. junin virus was recovered by cocultivation from peripheral blood mononuclear cells (p ... | 1993 | 8385886 |
| replication and physical parameters important for preparing purified junin virus. | junin virus (jv) is an arenavirus and the causative agent of argentine hemorrhagic fever (ahf), an often fatal human disease. the attenuated strain xj-clone 3 (xjc13) of jv, after being tested in humans, has been considered a promising vaccine. we found that synthesis of jv xjc13 reaches a peak 2 days after infection and the kinetics of synthesis are little affected by the multiplicity of infection (moi) in a range from 0.125 to 1.00. virus synthesis is sensitive to actinomycin d, indicating tha ... | 1993 | 8388396 |
| the effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of junin virus. | the effects of specific inhibitors of glycoprotein trimming reactions on junin virus (jv) replication were investigated. bromoconduritol, an inhibitor of glucosidase ii, significantly reduced infective virus production (de50: 1.1 mm) and viral protein expression. neither 1-deoxynojirimycin, an inhibitor of both glucosidases i and ii, nor 1-deoxymannojirimycin and swainsonine, inhibitors of mannosidase i and ii, respectively, showed any activity against jv multiplication. these results are the fi ... | 1993 | 8391500 |
| [present status of zoonotic hemorrhagic fevers of south america]. | since 1958, the geographical distribution of argentine hemorrhagic fever (ahf) has especially extended non only into the province of buenos aires but also towards the provinces of santa fe and cordoba, leading to an estimated population at risk of about 1.2 m inhabitants. recent epidemiological field studies has confirmed the major role of calomys musculinus and c. laucha rodents in both transmission to man and conservation of junin virus in nature. however, the human infection may result essent ... | 1993 | 7819800 |
| [present status of zoonotic hemorrhagic fevers of south america]. | since 1958, the geographical distribution of argentine hemorrhagic fever (ahf) has especially extended non only into the province of buenos aires but also towards the provinces of santa fe and cordoba, leading to an estimated population at risk of about 1.2 m inhabitants. recent epidemiological field studies has confirmed the major role of calomys musculinus and c. laucha rodents in both transmission to man and conservation of junin virus in nature. however, the human infection may result essent ... | 1993 | 7819800 |
| heterogeneity and stability characteristics of candid 1 attenuated strain of junin virus. | fourteen clones of candid 1 strain of junin virus were studied in terms of their attenuation and stability for 9 days-old mice. the cluster analysis of the efficiency of lethal infection, namely the virulence (lethality) indicates (pfu/ld50) of the clones showed that the strain population is heterogeneous. it is constituted by subpopulations with lower and higher virulence as compared to the parent strain. moreover, one or two passages through mice and calomys musculinus, the main junin virus re ... | 1993 | 8105651 |
| heterogeneity and stability characteristics of candid 1 attenuated strain of junin virus. | fourteen clones of candid 1 strain of junin virus were studied in terms of their attenuation and stability for 9 days-old mice. the cluster analysis of the efficiency of lethal infection, namely the virulence (lethality) indicates (pfu/ld50) of the clones showed that the strain population is heterogeneous. it is constituted by subpopulations with lower and higher virulence as compared to the parent strain. moreover, one or two passages through mice and calomys musculinus, the main junin virus re ... | 1993 | 8105651 |
| immunoperoxidase tracing of junin virus neural route after footpad inoculation. | to determine the pathway adopted by peripherally inoculated junin virus (jv) to reach the cns, rat tissues were serially harvested to trace the sequence of viral progression from right hind footpad to brain. immunoperoxidase (pap) labeling of viral antigen, concomitantly with infectivity assays and histological examination of each selected sample, were carried out. as from the 2nd week post-infection (pi), neurological disease inducing 100% mortality at 1 month was evident. at day 5 pi, viral an ... | 1992 | 1309637 |
| current progress towards vaccines for arenavirus-caused diseases. | the arenaviruses are primarily viruses of rodents, but some members of the group cause severe disease (argentine and bolivian haemorrhagic fevers and lassa fever) when transmitted to humans in the specific areas of the world where they are enzootic. current research of relevance to the provision of vaccines against these diseases, which highlights many of the problems encountered generally in the development of vaccines, is reviewed here. although one of the classical approaches to vaccine produ ... | 1992 | 1311492 |
| correlation between cyclophosphamide-induced viral susceptibility and depletion of junin virus-induced suppressor populations. | in contrast to lymphocytic choriomeningitis virus, another arenavirus, junin virus (jv), the etiologic agent of argentine hemorrhagic fever, when inoculated into suckling mice, induces lethal meningoencephalitis characterized by a delayed-type hypersensitivity (dth)-like immune response. however, the adult balb/c mouse is resistant to infection and no dth reaction can be seen. this different viral sensitivity may be related to the development of an antigen non-specific dth-suppressor cell pathwa ... | 1992 | 1315368 |
| experimental neuroinvasiveness of wild and laboratory junin virus strains. | the neuroinvasiveness of candid 1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus (cm), of different ages inoculated by a non-neural route. infectivity in brain, blood and organs, as well as lethality, were determined. the results with the 3 hosts indicate that junin virus neuroinvasiveness is virus-strain-dependent, host species- and age-dependent, with the candid ... | 1992 | 1329167 |
| experimental neuroinvasiveness of wild and laboratory junin virus strains. | the neuroinvasiveness of candid 1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus (cm), of different ages inoculated by a non-neural route. infectivity in brain, blood and organs, as well as lethality, were determined. the results with the 3 hosts indicate that junin virus neuroinvasiveness is virus-strain-dependent, host species- and age-dependent, with the candid ... | 1992 | 1329167 |
| detection of junin virus by the polymerase chain reaction. | argentine hemorrhagic fever is an often fatal human disease caused by junin virus, an rna-containing virus and member of the arenavirus family. this virus was detected in vitro by the polymerase chain reaction (pcr) procedure. a pair of junin virus-specific pcr dna oligonucleotide primers and an oligonucleotide probe were designed from a known portion of the viral rna sequence. rna was isolated from junin virus-infected monkey kidney cells and used to produce complementary dna (cdna) by reverse ... | 1992 | 1331150 |
| peripheral blood leukocytes of patients with argentine hemorrhagic fever as effectors of antibody-dependent cell-cytotoxicity. | peripheral blood mononuclear cells (pbmc) and polymorphonuclear leukocytes (pmnl) of patients with argentine hemorrhagic fever (ahf) were tested as effectors (e) of antibody-dependent cell cytotoxicity (adcc). 51cr labeled chicken red blood cells (crbc) coated with anti-crbc or normal rabbit serum were used as targets (t). three adcc assays were performed with both effectors from patients: on admission (i), 4 days after the transfusion of immune plasma (ii), and 30 days after the clinical onset ... | 1992 | 1331313 |
| peripheral blood leukocytes of patients with argentine hemorrhagic fever as effectors of antibody-dependent cell-cytotoxicity. | peripheral blood mononuclear cells (pbmc) and polymorphonuclear leukocytes (pmnl) of patients with argentine hemorrhagic fever (ahf) were tested as effectors (e) of antibody-dependent cell cytotoxicity (adcc). 51cr labeled chicken red blood cells (crbc) coated with anti-crbc or normal rabbit serum were used as targets (t). three adcc assays were performed with both effectors from patients: on admission (i), 4 days after the transfusion of immune plasma (ii), and 30 days after the clinical onset ... | 1992 | 1331313 |
| metal-based formulations with high microbicidal activity. | substances were evaluated for their relative potencies in inactivating junin virus, escherichia coli, and spores of bacillus subtilis. under the conditions of our test, glutaraldehyde was the most efficient agent among all substances currently recommended for disinfecting and sterilizing medical devices. either copper or iron ions by themselves were able to inactivate virus with an efficiency similar to that of substances currently used for disinfection and sterilization. the microbicidal effect ... | 1992 | 1332611 |
| a longitudinal study of junin virus activity in the rodent reservoir of argentine hemorrhagic fever. | we monitored junin virus (jv) activity in rodent populations for 30 months at seven mark-recapture grids located in agricultural fields and adjacent roadsides and fence lines in endemic and nonendemic areas of argentine hemorrhagic fever. blood and oral swabs taken from rodents captured at five-week intervals were analyzed by enzyme-linked immunosorbent assay for jv antigen (ag). calomys laucha and c. musculinus were the most frequently captured rodents, making up 47% and 22% of captures, respec ... | 1992 | 1335214 |
| a longitudinal study of junin virus activity in the rodent reservoir of argentine hemorrhagic fever. | we monitored junin virus (jv) activity in rodent populations for 30 months at seven mark-recapture grids located in agricultural fields and adjacent roadsides and fence lines in endemic and nonendemic areas of argentine hemorrhagic fever. blood and oral swabs taken from rodents captured at five-week intervals were analyzed by enzyme-linked immunosorbent assay for jv antigen (ag). calomys laucha and c. musculinus were the most frequently captured rodents, making up 47% and 22% of captures, respec ... | 1992 | 1335214 |
| candid no. 1 argentine hemorrhagic fever vaccine protects against lethal junin virus challenge in rhesus macaques. | the protective efficacy of candid no. 1, a live-attenuated vaccine against argentine hemorrhagic fever (ahf), was evaluated in non-human primates. twenty rhesus macaques immunized 3 months previously with graded doses of candid no. 1 (16-127, 000 pfu), as well as 4 placebo-inoculated controls, were challenged with 4.41 log10 pfu of virulent p3790 strain junin virus. all controls developed severe clinical disease; 3 of 4 died. in contrast, all vaccinated animals were fully protected; none develop ... | 1992 | 1338783 |
| candid no. 1 argentine hemorrhagic fever vaccine protects against lethal junin virus challenge in rhesus macaques. | the protective efficacy of candid no. 1, a live-attenuated vaccine against argentine hemorrhagic fever (ahf), was evaluated in non-human primates. twenty rhesus macaques immunized 3 months previously with graded doses of candid no. 1 (16-127, 000 pfu), as well as 4 placebo-inoculated controls, were challenged with 4.41 log10 pfu of virulent p3790 strain junin virus. all controls developed severe clinical disease; 3 of 4 died. in contrast, all vaccinated animals were fully protected; none develop ... | 1992 | 1338783 |
| aerosol infection of rhesus macaques with junin virus. | the purpose of our work was to determine if aerosols of junin virus can infect rhesus macaques and if the disease is the same as that produced by virus inoculated parenterally. the 6 macaques exposed to the virus by aerosol became acutely ill during the 3rd week after exposure, and all died. three died by day 21, while the remainder died after 1 month. junin virus was found primarily in visceral organs of those animals dying before 21 days after infection and in the central nervous system tissue ... | 1992 | 1371270 |
| aerosol infection of rhesus macaques with junin virus. | the purpose of our work was to determine if aerosols of junin virus can infect rhesus macaques and if the disease is the same as that produced by virus inoculated parenterally. the 6 macaques exposed to the virus by aerosol became acutely ill during the 3rd week after exposure, and all died. three died by day 21, while the remainder died after 1 month. junin virus was found primarily in visceral organs of those animals dying before 21 days after infection and in the central nervous system tissue ... | 1992 | 1371270 |
| cytotoxicity and cytoadherence of human and murine polymorphonuclear leukocytes against junin virus infected targets in the absence of anti-viral antibody and complement. | human and murine polymorphonuclear leukocytes (pmn) lysed l cells infected with junin virus (jv) in an in vitro system free of antiviral antibody and complement. infected vero cells proved to be resistant to the cytolytic effect. murine pmn showed an increased adherence on jv-infected l cells but did not attach to vero cells. the opposite was observed with human pmn, which did adhere to infected vero cells but not to infected l cells. these results indicate that pmn activity may be an early mech ... | 1991 | 1649909 |
| junin virus activity in rodents from endemic and nonendemic loci in central argentina. | small mammals were trapped during a 21-month period at 27 farm sites in 15 localities within and beyond the known endemic area for argentine hemorrhagic fever (ahf). prevalence of junin virus (jv) was assessed by antigen-capture enzyme immunoassay (elisa) on samples of body fluids and/or organs from 3, 282 captured rodents. infection in rodent populations was variable (0-3.7%) among localities but, in all cases, was lower than previously reported rates. overall prevalence was 1.4% in the ahf epi ... | 1991 | 1650148 |
| junin virus activity in rodents from endemic and nonendemic loci in central argentina. | small mammals were trapped during a 21-month period at 27 farm sites in 15 localities within and beyond the known endemic area for argentine hemorrhagic fever (ahf). prevalence of junin virus (jv) was assessed by antigen-capture enzyme immunoassay (elisa) on samples of body fluids and/or organs from 3, 282 captured rodents. infection in rodent populations was variable (0-3.7%) among localities but, in all cases, was lower than previously reported rates. overall prevalence was 1.4% in the ahf epi ... | 1991 | 1650148 |