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equine infectious anemia virus: development of a simple reproducible method for titrating infectivity of the cell-adapted strain.an equine dermal cell line between the 14th and 30th subpassages was used to develop a reproducible method of titrating the infectivity of the cell-adapted strain of equine infectious anemia virus (eiav). cells inoculated with eiav were subcultured or fed once each week and were monitored for the production of p26 antigen of eiav in supernatant fluids. ultrathin sections were prepared once each week and were examined for the detection of budding virus-like particles (vlp). the vlp could not be d ...1979223479
leukocyte cytotoxicity in a persistent virus infection: presence of direct cytotoxicity but absence of antibody-dependent cellular cytotoxicity in horses infected with equine infectious anemia virus.antibody-dependent cellular cytotoxicity and direct cytotoxicity assays were performed with equine infectious anemia virus-infected target cells, equine leukocytes, and equine anti-equine infectious anemia virus antibody to determine whether these mechanisms play a role in controlling viral replication in equine infectious anemia. direct cytotoxicity was observed by using peripheral blood mononuclear cells from 7 of 10 infected horses. antibody-dependent cellular cytotoxicity was not observed. t ...1979223981
responses in horses infected with equine infectious anemia virus adapted to tissue culture. 1979228570
characterization of the infection of equine fibroblasts by equine infectious anemia virus.equine dermal fibroblasts persistently infected with equine infectious anemia virus (eiav) show no alterations in cell morphology or growth kinetics when compared to uninfected cells. the percentage of cells immunofluorescent positive for viral proteins fluctuated, depending upon the stage of the cell cycle, while production of extracellular virus was uniform throughout the cell cycle, increasing only as the cell number increased. this was shown in log versus stationary phase cultures as well as ...1979228638
failure to propagate equine infectious anemia virus in mosquitoes and culicoides variipennis.laboratory-colonized mosquitoes, culex tarsalis, aedes aegypti, culiseta inornata, and anopheles free-borni, and the biting gnat, culicoides variipennis, were exposed to equine infectious anemia virus. exposure to the virus was by intrathoracic inoculation for mosquitoes and by oral ingestion of an infective blood meal through a membrane for c variipennis. after various intervals, groups of 15 to 20 insects were homogenized and inoculated into susceptible ponies. positive immunodiffusion test r ...197831831
rna-dependent dna polymerase associated with equine infectious anemia virus.equine infectious anemia (eiav) is shown to have an associated rna-instructed dna polymerase similar in its cofactor requirements and reaction conditions to the rna tumor virus dna polymerases. demonstrating this dna polymerase activity requires a critical concentration of a nonionic detergent, all four deoxyribonucleoside triphosphates, and a divalent metal ion. the reaction is sensitive to rnase, and a substantial fraction of the fna synthesized is complementary to viral rna. the detection of ...197767219
purification of equine infectious anemia virus antigen by affinity chromatography.affinity chromatography was performed to obtain highly purified antigen from equine infectious anemia (eia) virus. after crude antigen was concentrated by polyethylene glycol precipitation of culture fluids from equine dermal cells persistently infected with eia virus, and after the virus was disrupted with ether, it was added to a column of cyanogen bromide-activated sepharose 4b to which eia-specific antibody had been conjugated. the antigen was effectively released from the column with 5m mgc ...197769631
eia research. 1977189175
viral antigen production in horse kidney cell cultures infected persistently with equine infectious anemia virus. 1976177893
equine infectious anaemia. 1976181892
mechanism of viral persistence in equine infectious anemia. 1975165036
[latest results in the research of infectious anemia in perissodactyla]. 1975174243
spontaneous autoimmune diseases of domestic animals. 19744367527
equine infectious anemia virus from infected horse serum.equine infectious anemia virus was purified from infected horse serum samples. electron microscope observation on negatively stained preparations of purified virus showed roughly spherical particles sized between 100 and 200 nm in diameter. in disrupted particles, an envelope was visible but no internal structure could be resolved. since the purified virus fraction had a strong antigenic activity to antiserum in immunodiffusion reaction, these particles are thought to be the causative virus of e ...19744372175
passive immunity in the foal: measurement of immunoglobulin classes and specific antibody. 19734355952
role of horse fly (tabanus fuscicostatus hine) and stable fly (stomoxys calcitrans l.) in transmission of equine infectious anemia to ponies in louisiana. 19734357708
preparation of equine infectious anemia antigens for diagnosis. 19734128832
diagnosis of equine infectious anemia by immunodiffusion test. 19724333633
demonstration of antigenic identity between purified equine infectious anemia virus and an antigen extracted from infected horse spleen.antigenic relationship between purified equine infectious anemia (eia) virus and spleen-derived antigen from eia-infected horses was examined by immunodiffusion. identical antigenicity of these two antigens has been proven because precipitation lines formed between the two antigens and eia antiserum connected with each other. the results indicate that the antigenic substance derived from infected spleen is a component of eia virus.19724629262
immunofluorescent localization of equine infectious anemia virus in tissue. 19714322275
characterization of precipitating antibody in equine infectious anemia. 19714328472
detection of equine infectious anemia virus in vitro by immunofluorescence. 19714330258
titration of equine infectious anemia virus. effect of dosage on incubation time and clinical signs. 19714330637
immunodiffusion studies of purified equine infectious anemia virus.antigenicity of purified equine infectious anemia (eia) virus was examined by immunodiffusion against sera obtained from horses experimentally infected with eia virus. the purified virus reacted with the infected horse serum, and virus-specific precipitating antibody was demonstrated. furthermore, it was found that purified eia virus reacted against the serum of horses infected with all strains of eia virus which were antigenically different from one another. from the result, group-specific comp ...197116557982
[cultivation of equine infectious anemia virus]. 19704325782
equine infectious anemia: preliminary investigation of the complement-fixation test for the demonstration of antibodies and antigen.clinical field cases of equine infectious anemia were studied and the disease was reproduced experimentally in horses. attempts were made to adapt the complement-fixation test to the detection of antibodies in the serum of infected animals and to the demonstration of antigens in tissue extracts.a moderate complement-fixing antibody response was demonstrated in the serum of horses shortly after primary exposure to the infectious agent. however, this reactivity was of short duration and occurred w ...19694305760
viremia and immunological responses in horses infected with equine infectious anemia virus. 19694306393
[myocardium infarct in horses with infectious anemia]. 19684300496
pathological studies on bone marrow in equine infectious anemia. 3. cytlogical findings of bone marrow aspirates. 19684182535
[the development of petechial hemorrhages on the under-surface of the tongue in the horse and its relation to infection with the virus of equine infectious anemia]. 19674299474
attempts to adapt equine infectious anemia virus to small animals. 195014792980
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