Publications

endocrine effect of a methionine-enkephalin derivative (fk 33-824) in man.	the endocrine profile of fk 33-824, 0.5 mg i.m., was determined in comparison with placebo or pretreatment with naloxone, 4 mg i.v., in 14 healthy men. prolactin (prl), growth hormone (gh), acth, cortisol, gonadotropins (lh, fsh), thyrotropin (tsh), thyroxine (t4), triiodothyronine (t3), insulin and glucagon were measured and free water clearance was calculated from urine volumes and osmolar clearances. fk 33-824 increased prl and gh (p less than 0.001). acth was below assay sensitivity but cort ...	1980	6262206
nucleosome structure and conformational changes.	we have used a variety of chemical probes to measure the accessibility of dna on the surface of the nucleosome. we review these results, and describe new experiments which show that t4 phage dna can form complexes with the core histones, possessing the properties of normal nucleosomes. since t4 dna is largely occupied by glucose residues in the major groove, this suggests (as did earlier probe experiments) that the major groove is not filled with histone amino acid side chains. we also report re ...	1980	6264987
on the thermodynamics and kinetics of the cooperative binding of bacteriophage t4-coded gene 32 (helix destabilizing) protein to nucleic acid lattices.	in this paper we summarize a series of thermodynamic, and preliminary kinetic, studies on the molecular details and specificity of interaction of phage t4-coded gene 32-protein (gp32) with nucleic acid lattices. it is shown that the binding of gp32 to short (l = 2--8 residues) oligonucleotides is essentially independent of base composition and sugar-type, as well as of salt concentration. in contrast, cooperative (continuous) or isolated binding of gp32 to single-stranded polynucleotides is base ...	1980	6264988
t4 rna ligase as a nucleic acid synthesis and modification reagent.	oligodeoxyribonucleotides corresponding to portions of the recognition sequence and analogues thereof of the eco ri restriction endonuclease have been synthesized using t4 rna ligase. the successive addition of deoxyribonucleoside-3',5'-bisphosphates to preformed deoxyoligomers allowed stepwise oligodeoxyribonucleotide synthesis. single strand deoxyoligomers were also joined to one another by the enzyme. in addition, biotin, and fluorophore tetramethylrhodamine, and hexylamine have been added to ...	1980	6265872
dose dependence of the thyrotropin (tsh) receptor damaging effect of gonadotropin in the newborn rats.	a single gonadotropin treatment of newborn rats influenced the thyrotropin (tsh) provoked thyroxine (t4) production of 2 months old animals. on pretreatment with 10, 20, 50, 100 and 200 i.u. of gonadotropin, the t4 blood level of animals given 10 and 200 i.u. differed scarcely from the controls treated only by tsh, while intermediary doses had a damaging effect as the t4 values were well below the control values measured in animals treated by tsh in adulthood.	1980	6269348
[hormonal stimulation after injection of thyrotrophin releasing factor (trf) and 1-24 acth in patients following chronic treatment with neuroleptics (author's transl)].	fifteen patients with neuroleptic treatment (haloperidol 4 to 6 mg a day or phenothiazines) and eleven control patients were given trh 250 microgram i.v. and 1-24 acth, i.m. different times, tsh, t3, t4, ft4, prolactin (prl) cortisol were measured. before trh injection, at baseline, tsh is higher (p less than 0,01) and t3 lower (p less than 0,05) in the neuroleptics treated group than in the control group. these results suggest the occurrence of a biological hypothyroidism in the group with neur ...	1980	6263049
contractile, biochemical, and histochemical properties of thyrotoxic rat soleus muscle.	the effects of thyrotoxicosis on the contractile properties of soleus muscle were examined in rats given 3 mg of t4 and 1 mg of t3 per kg of diet for 6--8 wk. thyrotoxicosis induced significant decreases in isometric twitch contraction time (ct), one-half relaxation time, and peak twitch tension. the ca2+ uptake activity of the sarcoplasmic reticulum (sr) was increased in the thyrotoxic muscles; this adaptation in the sr provides a possible mechanism for the alterations in isometric contractile ...	1980	6444499
interrelationships between energy metabolism and thyroid hormone metabolism during starvation in the rat.	thyroid hormone metabolism and plasma concentrations of tsh were studied after short-term hypocaloric refeeding of rats starved for 2-6 days. carbohydrate and protein (10 kcal) refeeding after 4 days of starvation resulted in a rapid increase in serum t3 (p less than 0.01) and, less consistently of t4. plasma tsh did not change. these findings were not due to changes in the metabolic clearance rates or in thyroid hormone binding proteins, as the disappearance of injected labelled t3 and t4, and ...	1980	6445670
loss of suppressor t cells in active multiple sclerosis. analysis with monoclonal antibodies.	to determine whether abnormalities of immunoregulatory t cells are associated with multiple sclerosis (ms), we characterized peripheral lymphocytes in 33 patients with untreated ms and compared them with 42 normal persons and 29 age-matched control subjects who had other neurologic diseases. for this analysis, we used monoclonal antibodies to the surface antigens of helper (t4) and suppressor (t5) t-cell subsets and to a common t-cell antigen (t3). in contract to normal persons and the controls ...	1980	6446039
[concentrations of serum iodothyronines during starvation in genetically obese mice].	examination of the thyroid hormones levels of genetically obese mice (ob /ob) and their lean littermates (ob/-) revealed high concentration of 3,5,3' triiodo-l-thyronine (t3) in obese mice reflecting probably an increased of peripheral l-thyroxine (t4) deiodination to t3. during starvation, peripheral t4 metabolism is shunted from activating (t3) to inactivating pathway (3,3',5' triiodo-l-thyronine, rt3) in both groups of animals.	1980	6446954
region-specific recombination in phage t4. i. a special glucosyl-dependent recombination system.	in this communication, we describe a recombination mechanism in bacteriophage t4d that acts only on glycosylated phage, acts in some regions of the genome, but not others, and is heat sensitive, showing decreasing activity with increasing temperature.	1980	6447095
region-specific recombination in phage t4. ii. structure of the recombinants.	in this paper, we present results of crosses designed to elucidate the structure of recombinants in the tail-fiber region of bacteriophage t4, in which a glucosylation-dependent recombinations mechanism is operative, and the cause of the "special" recombination in glycosylated crosses is discussed. we present evidence that, when phage are nonglycosylated, recombination in the tail-fiber region proceeds via long heteroduplex overlaps. mismatched bases within such regions (in nonglycosylated phage ...	1980	6447096
region-specific recombination in phage t4. iii. the effect of host mutations on glucosylation-dependent recombination in bacteriophage t4d.	mutations in the escherichia coli genes reck, recl and (probably) uvre and pola increase special (glucosylation-dependent), but not general recombination in bactriophage t4d.	1980	6447097
subunits of the adenosine triphosphatase complex translated in vitro from the escherichia coli unc operon.	the unc operon of escherichia coli was split into two fragments by the restriction endonuclease hindiii. the operator-proximal portion was cloned into plasmid pacyc184, forming plasmid pan51, which included the genes uncb, unce, and unca. when plasmid pan51 was used as template in an in vitro transcription/translation system, the alpha subunit (from the unca gene) and delta subunit of the f(1) adenosine triphosphatase (atpase) were formed. in addition, three polypeptides of molecular weights 18, ...	1980	6447144
shutoff of lambda gene expression by bacteriophage t4: role of the t4 alc gene.	bacteriophage t4 normally contains 5-hydroxymethylcytosine instead of cytosine in its dna. multiple mutants of t4 which synthesize dna with cytosine do not transcribe their late genes due to the action of the t4 alc gene (snyder et al., proc. natl. acad. sci. u.s.a. 73:3098--3102, 1976), which is also responsible for unfolding the host nucleoid after t4 infection (sirotkin et al., nature [london] 265:28--32, 1977; tigges et al., j. virol. 24:775--785, 1977). it seems reasonable that t4 alc funct ...	1980	6447802
subnormal plasma adrenal androgen levels in men with uremia.	the 24-h mean plasma concentrations of 8 hormones were measured in 11 men with chronic uremia and 32 normal men. our findings confirm previous reports of subnormal levels of testosterone, t3, and t4 and elevated levels of lh, prl, and cortisol. in addition, we observed a new finding: markedly subnormal levels of the adrenal androgens dehydroisoandrosterone (dha) and dha sulfate. the mean dha level in the patients was 164 +/- 46 (sd) ng/dl, compared with 320 +/- 124 in age-matched controls (p < 0 ...	1980	6448261
characterization of a rho-dependent termination site within the cro gene of bacteriophage lambda.	bacteriophage lambda transcripts were synthesized in vitro using lambda b2 dna and escherichia coli rna polymerase. rna molecules initiating with atp were labeled exclusively at the 5'-end by transcribing in the presence of [gamma-32p]atp. they were analyzed by electrophoresis in 3.5% polyacrylamide, 7.5 m urea gels followed by autoradiography. the previously known 6 s rho-independent transcript and rho-dependent 9 s and 8 s transcripts were observed. a new rho-dependent 5 s transcript was also ...	1980	6448859
plasma dehydroepiandrosterone sulphate in hypothyroid premenopausal women.	plasma dehydroepiandrosterone sulphate (dheas), cortisol (f) and prolactin (prl) were measured in seventeen premenopausal women with primary hypothyroidism and in fifteen normal premenopausal women. significantly lower (p < 0.001) dheas levels were found in hypothyroid women while f and prl were in the range of normal controls. no significant relation was found between dheas and total thyroxine (t4) and tsh. our results support the hypothesis that dheas secretion is impaired in some women with p ...	1980	6449317
cleavage of phi x174 single-stranded dna by gene a protein and formation of a tight protein-dna complex.	the gene a protein cleaves phi x174 single-stranded dna (ssdna). the cleavage appears to be stoichiometric, whereby a gene a protein molecule breaks a phosphodiester bond and binds to the 5' end. the enzyme introduces mostly a single break in a circular ssdna molecule. however, at high enzyme-to-dna ratios, more than one break in the dna could be observed. the cleavage of the ssdna by gene a protein renders the dna sensitive to the action of terminal transferase to incorporate [alpha -32p]atp. t ...	1980	6449606
restoration of ligase activity in e. coli k12 lig ts7 strain by bacteriophage mu and cloning of a dna fragment harbouring the mu 'lig' gene.	restoration of ligase activity has been observed in e. coli k12 ligts7 strain lysogenic for mu, in presence as well in absence of lysogenic immunity. this restoration consist in phenotypic reversal of temperature sensitivity of e. coli ligts7 which also regain the ability to sustain the complete growth cycle of t4 lig-phages. it is possible to put under the control of the gal operon the expression of the viral gene responsible for the restoration effect. this new gene of mu has been named 'lig'. ...	1980	6449688
changes in serum n-acetyl-beta-hexosaminidase levels after treatment of hypothyroid and hyperthyroid individuals with l-thyroxine and propylthiouracil.	multiple serum samples were obtained from six hypothyroid and six hyperthyroid females, 11--17 years of age, over the course of their corrective treatment with l-thyroxine (lt4) and propylthiouracil (ptu), respectively. sera were assayed for total n-acetyl-beta-hexosaminidase (hex), the a (heat-labile) and b (heat-stable) isozymes, and total t4. hex activity (total hex a) in sera from hypothyroid (< 4 micrograms/dl t4) patients (total hex: 518 +/- 66 nmol/60 min/ml, mean +/- s.d.; hex a: 325 +/- ...	1980	6450007
thyrotropin-releasing hormone-degrading activity of neonatal human plasma.	thyroid physiology changes rapidly during the neonatal period. thus, there is an abrupt release of tsh which peaks within 30 min after birth and there are significant increases in the plasma concentrations of t3 and t4, which peak 24--48 h after birth. in the present work, high pressure liquid chromatography was used to study the evolution of trh-degrading activity in the plasma of newborns during the first 5 days of life. trh-degrading activity is initially absent in cord blood and appears afte ...	1980	6765954
decreased serum 3,5,3'-triiodothyronine and thyroxine levels accompanying acute and chronic ritalin treatment of developing rats.	basal serum levels of t3, t4, and tsh and the serum tsh increment after trh are reported for rats chronically or acutely treated with ritalin. serum levels of t3 and t4 are significantly reduced in rats chronically treated with ritalin twice daily for 21 days. female rats appear to be more susceptible than males to the suppressive action of ritalin. ritalin appears to accelerate the circulatory clearance of serum t3 and t4. basal serum levels of tsh demonstrate graded elevations after sequential ...	1980	6766387
triac (3,5,3'-triiodothyroacetic acid) partially inhibits the thyrotropin response to synthetic thyrotropin-releasing hormone in normal and thyroidectomized hypothyroid patients.	the effects of a daily oral dose (1.4 mg) of 3,5,3'-triiodothyroacetic acid (triac) on thyroid hormone levels (t4, t3 and rt3) and on the tsh and prl responses to trh were studied in 15 normal subjects and 5 hypothyroid patients. there were no significant changes in weight, heart rate, reflex time, or serum concentration of either cholesterol or triglycerides after 6 weeks of triac administration. however, t4 was significantly reduced to a lower mean level (mean +/- sem, 7.3 +/- 0.7 to 4.3 +/- 0 ...	1980	6766460
seasonal changes in the thyroid hormones of the male squirrel monkey.	the serum of male squirrel monkeys was analyzed for levels of thyroxine (t4) and triiodothyronine (t3) at intervals corresponding to (1) preparations for breeding, (2) termination of breeding, and (3) well after the breeding season. highest concentrations of hormones were found prior to breeding when body weight was also highest. significant differences in hormone levels were found across sampling period. the interaction between gonadotropins and thyroid hormones is discussed.	1980	6767454
thyrotoxicosis without elevated serum triiodothyronine levels during diabetic ketoacidosis.	thyrotoxicosis with a normal serum triiodothyronine (t3) concentration has been described with a variety of acute and chronic illnesses occurring in association with thyrotoxicosis. we describe the first case to our knowledge of thyroxine (t4) toxicosis in a 16-year-old boy with diabetic ketoacidosis. although the clinical manifestations of hyperthyroidism were mild, thyromegaly and persistent tachycardia suggested thyrotoxicosis. serum t4 levels were elevated; however, the serum t3 level was no ...	1980	6767458
temporal dissociation of late events in bacillus subtilis sporulation from expression of genes that determine them.	during sporulation in replacement medium, resistance to toluene to heating at 65 degrees c, to lysozyme, and to heating at 80 degrees c appeared in sequence between 4 and 8 h after the induction of sporulation (i.e., between t4 and t8). the addition of sufficient chloramphenicol at t4.5 to prevent protein synthesis nevertheless allowed the emergence of all of these types of resistance except lysozyme resistance. the numbers of spores with these types of resistance (lysozyme resistance again exce ...	1980	6767691
prostaglandin in human breast cancer: evidence suggesting that an elevated prostaglandin production is a marker of high metastatic potential for neoplastic cells.	prostaglandin (pg) production by human breast cancers was investigated in 91 lesions selected so that the distribution of histologic type was similar to that of the general population of mammary carcinomas. with regard to the shape characteristics of the tumors, pg production was higher in lesions classified t1 and t2 than in lesions classified t3 and t4 (t-classification is based on extent of tumor as graded by the international union against cancer), higher in tumors exhibiting a high cellular ...	1980	6767871
[use of an osmotic minipump for the study of thyroxine deiodination in the rat].	thyroidectomized rats were implanted with an osmotic minipump delivering a continuous but small amount of t4 (2 microgram/100 g/day), mimicking therefore thyroid secretion. this experimental procedure allows a normal serum t3 level to be maintained by peripheral deiodination of t4.	1980	6768463
primary biliary cirrhosis: the prevalence of hypothyroidism and its relationship to thyroid autoantibodies and sicca syndrome.	a survey of thyroid function in 95 patients with primary biliary cirrhosis revealed the presence of thyroid antibodies in 24 females and 1 male. thirteen (52%) of this thyroid antibody positive group (all female) had biochemical evidence of thyroid disease: 8 (32%) were hypothyroid (7 of whom showed signs of myxedema), 2 had elevated thyroid stimulating hormone (tsh) levels, and 3 had abnormal responses to stimulation with thyrotropin releasing hormone (trh). in contrast, only 3 (4%) of the thyr ...	1980	6768634
interactions of homologous and heterologous deoxyribonucleic acids and competent bacillus subtilis cells.	glucosylated and nonglucosylated bacteriophage t4 deoxyribonucleic acids (dnas) are able to bind to competent cells of bacillus subtilis, although the former does so in a rather unstable fashion, probably because of the glucosylation. several heterologous dnas compete with homologous dna for the same receptors in binding and in transformation. a different pattern in competition for dna binding was observed for homologous and t4 glucosylated dnas in intact cells as compared with protoplasts or me ...	1980	6768709
alterations in circulating thyroid hormones and thyroxine-binding globulin levels during diabetic ketoacidosis.	during diabetic ketoacidosis, in 17 adult patients, significant decreases in serum tbg and total t4 levels were observed, without significant alteration of the t4 to tbg binding property. in addition, serum free t4 (ft4) was moderately elevated and the tsh response to trh was markedly blunted. no correlation, however, was found between tsh blunting and ft4 elevation. correlation of these serum anomalies required at least 5 days of adequate control of the diabetes. thus, diabetic ketoacidosis in ...	1980	6768790
effect of chronic treatment with thyrotropin-releasing hormone (trh) or an analog of trh (linear beta-alanine trh) on the hypothalamic-pituitary-thyroid axis.	the effects of treatment for 5 or 9 days with varying doses of thyrotropin-releasing hormone (trh) or the linear beta-alanine trh congener (pglu-his-pro-beta-ala-nh2) on serum levels of tsh, t3, and t4 were studied in mice and rats. at low doses in rats treatment with trh for 9 days significantly increased serum levels of t3 but not serum t4 whereas a higher dose of trh (10 mg/kg) reduced serum t3 levels. beta-ala trh (0.1--10 mg/kg ip) treatment for 9 days in rats significantly reduced serum t4 ...	1980	6769064
effects of dietary ascorbic acid, aspirin, lysine, and thiouracil on thyroid activity.	broiler chickens were reared in batteries and fed diets designed to determine the effects of either ascorbic acid, aspirin (acetylsalicylic acid), lysine, or thiouracil on thyroid weight and serum thyroid hormone concentrations. thyroxine (t4) and 3,5,5'-triiodothyronine (t3) concentrations in serum were determined by radioimmunoassay. neither ascorbic acid nor lysine affected t3 or t4 concentration, but thiouracil significantly reduced t3 concentration after 1 day and reduced t4 concentration a ...	1980	6769103
thyroid gland function during childhood and adolescence. changes in serum tsh, t4, t3, thyroxine-binding globulin, reverse t3 and free t4 and t3 concentrations.		1980	6769275
effect of propranolol on serum t3, t4 and tsh, and the trh induced t3, t4 and tsh responses in euthyroid men.		1980	6769795
high-molecular-weight antigenic protein complex in the outer membrane of neisseria gonorrhoeae.	the outer membrane of neisseria gonorrhoeae contains approximately 15 proteins, with 2 or 3 accounting for over 75% of the total protein mass. samples of outer membrane from strain 2686 t4 analyzed by electrophoresis in 2% polyacrylamide gels revealed a band with an apparent molecular weight of 800,000. the band was protein material, as indicated by trypsin and pronase sensitivity and by l-[3h]proline incorporation. peptidoglycan, nucleic acids, and carbohydrate were not detected in the band. dy ...	1980	6769802
grades of subclinical hypothyroidism in asymptomatic autoimmune thyroiditis revealed by the thyrotropin-releasing hormone test.	serum levels of t4 t3, and tsh and peak tsh after trh administration were determined in 60 female subjects affected with asymptomatic autoimmune thyroiditis (aat), in 27 normal subjects, and in 8 myxoedematous patients used as controls. the aat subjects were divided into 3 groups on the basis of their basal and peak tsh values. in the first group (grade i aat), these parameters were similar to those of the normal controls; in the second (grade ii aat), basal tsh remained normal but peak tsh was ...	1980	6769939
a study of the relationship between thermogenesis and thyroid hormones.	to investigate the relationship between serum t3 levels and thermogenesis in euthyroid subjects, the conversion of t4 to t3 was partially blocked over a period of 3 weeks by weekly ingestion of 2 g iopanoic acid. serum t3 decreased by 56 +/- 6 ng/100 ml without affecting the metabolic rate or total heat loss. because an increase in serum t4 levels was observed (3.70 +/- 0.2 microgram/100 ml), the experiment was repeated in t4-substituted subjects. serum t3 decreased even more in these subjects, ...	1980	6769943
determination of serum thyroxine (t4), triiodothyronine (t3) and t4: t3 ratio by weight in normal subjects and patients with various thyroidal and nonthyroidal illnesses.		1980	6770032
an assessment of methods for the estimation of free thyroxine.	three commercial kit methods for the estimation of free thyroxine (ft4) (clinical assays, corning medical, damon diagnostics) were evaluated. the effects of changes in thyroxine-binding globulin (tbg) and ft4 concentrations in these systems were assessed. measurements were made in serum samples from hyperthyroid, hypothyroid, and euthyroid patients with different tbg concentrations, and results were correlated with functional thyroid status. the clinical assays and damon diagnostics assays were ...	1980	6770056
[correlations of free t4 index with t4: tbg ratio and of free t3 index with t3: tbg ratio (author's transl)].		1980	6770135
circulating fsh, lh, gh, testosterone, tsh, t3, t4, prolactin and insulin in boys during puberty.	the dynamics of the serum fsh, lh, gh, t, tsh, t3, t4, prolactin (prl) and insulin levels was studied in 50 healthy boys aged between 8 and 18 years, in correlation with the development of the gonad (sexualization) assessed through the five-stage classification (g i-g v) proposed by tanner. the results reveal the role of gonadotropins (especially lh) and testosterone in the growth and development of the child and especially in the sexualization process through a progressive increase from stage g ...	1980	6770454
thyroid-pituitary function in eight anencephalic infants.	the function of the thyroid pituitary axis was investigated in 8 anencephalic infants with no hypothalamus. thyrotrophin (tsh), thyroxine (t4), 3,5,3'-triiodothyronine (t3) and 3,3',5'-triiodothyrone (reverse t3 and rt3) were measured in the cord blood in 5 cases and during the first 4 h of life in 3 cases. tsh response to synthetic thyrotrophin-releasing hormone (trh) (200 microgram iv) was carried out in two cases and thyroid hormone response to bovine tsh (5 iu iv) was evaluated in 3 cases. t ...	1980	6770566
oral administration of trh in puerperal women: effect on insufficient lactation, thyroid hormones and on the responses of tsh and prolactin to intravenous trh.	thirteen puerperal women with insufficient lactation were treated with thyrotrophin releasing hormone (trh) 20 mg twice daily for two weeks. intravenous (iv) trh stimulation tests were done before the trh therapy and within 3-5 h after the last dose of oral trh. plasma samples were assayed for prolactin (prl), thyrotrophin (tsh), triiodothyronine (t3) and thyroxine (t4) by radioimmunoassays, and the lactational response was objectively monitored in 11 women. oral trh treatment was associated wit ...	1980	6770567
clofibrate and a related compound suppress tsh secretion in primary hypothyroidism.	a single oral dose of the hypolipidaemic agent ethyl-p-chlorophenoxyisobutyrate (clofibrate, 750 mg) produced a significant reduction on the high basal serum tsh level in patients with primary hypothyroidism. there was no consistent change in serum levels of thyroxine-iodine (t4-i), triiodothyronine (t3) and per cent free t4 (%ft4) during the study. on the other hand, clofibrate failed to produce discernible changes in the basal and trh-induced tsh secretion in euthyroid subjects. similar result ...	1980	6770570
a method for the preparation of human thyroxine-binding globulin; its importance in the establishment of an accurate and specific radioimmunoassay.	pure thyroxine-binding globulin (tbg) is required in radioimmunoassay to prepare monospecific antisera, [125i]tbg and as primary standard. homogeneous tbg was prepared by a three-stage affinity chromatography procedure; it could not be dissociated into subunits and its molecular weight by sds polyacrylamide gel electrophoresis was 59 000. the amino acid composition was in agreement with two earlier reports. the secondary structure determined by circular dichroism in the far u.v. showed it to con ...	1980	6771062
the effect of calorie restriction on serum thyroid hormone binding proteins and free hormone in obese patients.	in ten obese euthyroid subjects the concentration of thyroxine (t4), tri-iodothyronine (t3) and reverse t3 (rt3) were assayed in serum and of t4 and t3 in urine before and after 2 weeks of 2.1 mj (500 kcal) per day diet. mean serum t4 was unchanged, while t3 decreased and rt3 increased. urinary excretion of both t4 and t3 decreased after diet. in six subjects the concentrations of serum thyroxine binding globulin (tbg), thyroxine binding prealbumin (tbpa) and albumin were measured before and aft ...	1980	6771066
[clinical usefulness of the t4/tbg ratio in the diagnosis of thyroid dysfunction].		1980	6771923
phenolic ring deiodination in cultured rat hepatoma cells, and subcellular localization of deiodinases in cultured rat hepatoma, monkey hepatocarcinoma cells and normal rat liver homogenates.	the cultured rat hepatoma cell (r117--21b) homogenates metabolized 3,[3',5'-125i]triiodothyronine by phenolic ring deiodination and produced radioactive iodide and 3,3'-diiodothyronine. thyroxine (t4) was converted to 3,3',5-triiodothyronine (t3). the production of 125i- represented the deiodination was observed to be ph 6.0--7.0. this enzyme reaction was accelerated by dithiothreitoil. propylthiouracil strongly inhibited the phenolic ring deiodination at 0.1 mm, whereas an effect of 20 mm methy ...	1980	6772233
is growth hormone the pituitary feminizing factor mediating the actions of estradiol on hepatic drug and steroid metabolism?	previous studies have established that the effects of estradiol (e2) on hepatic steroid and drug metabolism are demonstrable only in the presence of the pituitary gland. studies were carried out to test the hypothesis that gh is the pituitary feminizing factor mediating the actions of e2 on hepatic metabolism. e2 and gh administered to castrated male rats had similar effects on hepatic enzymes, decreasing the oxidataive metabolism of drugs [ethylmorphine demethylation, aniline, hydroxylation, an ...	1980	6772428
[growth patterns of neisseria gonorrhoeae: occurrence and significance].	the introduction of transparent mediums for the growth of the gonococcus in the laboratory led to a definitive examination of colonial morphology. characteristics, such as size, elevation, colour, highlights, opacity,. consistency and evaluation of edges make possible to distinguish five colonial types: t1-t5. the most significant characteristic associated with colony morphology has been virulence; t1/t2-colonies are virulent for humans and t3/t4-colonies are avirulent. interactions with host ce ...	1980	6772601
the effect of iopanoic acid on the regulation of thyrotropin secretion in euthyroid subjects.	oral cholecystographic agents induce a decrease in serum t3 and an increase in serum t4 and rt3 concentrations in normal subjects. however, their effect on tsh secretion in man is unclear. in the present study, the serum tsh concentration was increased above baseline values 5 days after the administration of 3 g iopanoic acid (ia) in five of six euthyroid volunteers (an increase of borderline significance), and the tsh response to trh was significantly augmented after ia administration. in four ...	1980	6772664
effect of thyroid hormones on gonadotrophin release and biosynthesis using rat pituitary cell cultures.	the effect of thyroid hormones, tri-iodothyronine (t3) and thyroxine (t4) on the gonadotrophin releasing hormone (gnrh)-induced release of lh and fsh has been studied using dispersed rat pituitary cell cultures. both t3 and t4 significantly inhibited the release of gonadotrophins in the presence of gnrh. actinomycin d and cycloheximide also inhibited synergistically the release of lh and fsh. the incorporation of labelled amino acids and glucosamine into lh and fsh which was stimulated by gnrh w ...	1980	6772729
thyroid hormone homeostasis in states of relative caloric deprivation.	starvation is accomplished by significant changes in the hypothalamic-pituitary-thyroid axis and in peripheral thyroid hormone metabolism. less well studied, however, are the effects on thyroid hormone economy produced by hypocaloric feeding. we explored these changes in obese patients fed 200, 400, or 600 cal/day of either carbohydrate of protein for 28 days. t4' t3' reverse t3 and the tsh response to trh were measured at frequent intervals. each patient demonstrated a transient rise in reverse ...	1980	6772921
[lactation regulation and the content of thyroid hormones in the milk of parturients and components of infant food formulas].	the content of folliculostimulating (fsh), luteinizing (lh), thyrotropic (tth) hormones and prolactin was studied in the blood serum of pregnant women and puerperants; also the thyroxin (t4), triiodthyronin (t3), thyroxin-binding globulin (tbg) levels, and the thyroxin-binding capacity of proteins and free t4 index were determined in the blood serum and milk of puerperants during the first week of lactation. the blood serum fsh, lh, tth and prolactin concentrations underwent definite changes con ...	1980	6773040
inhibitory effect of unlabeled iodothyronines on the deiodination of labeled thyroid hormones by cultured hepatocarcinoma cells.	inhibitory effects of unlabeled iodothyronines on the metabolism of thyroxine (t4), 3,3',5-triiodothyronine (t3) and 3,3',5'-triiodothyronine (reverse t3, rt3) were investigated in continuously cultured monkey hepatocarcinoma cells which showed a rapid metabolism of the thyroid hormones. nonphenolic ring deiodination of [3',5'-125i]-t4 and [3'-125i]-t3 was strongly inhibited by excess t3, 3,5-diiodothyronine (3,5-t2) and t4, whereas rt3 was the least effective inhibitor. phenolic ring deiodinati ...	1980	6773753
[normality of serum thyroid hormones, thyroxine binding globulin and thyroid stimulating hormone concentrations in normal adult men (author's transl)].	the normality of serum thyroxine (t4), triiodothyronine (t3), reverse t3, thyroxine binding globulin (tbg) and thyroid stimulating hormone (tsh) concentrations were investigated by using normal probability paper. 107 healthy adult men (age 35-48) were carefully selected; their healthiness was evaluated by physical, biochemical and roentogenologic examinations as well as by intensive interview on their past history. all assays were simultaneously carried out. when each cumulative distribution was ...	1980	6773867
multiple abnormalities of anterior pituitary hormone secretion in association with pseudohypoparathyroidism.	a male with pseudohypoparathyroidism presented with several hormonal abnormalities. he was clinically euthyroid with no palpable goiter, his serum t4, total t3, t3 sephadex retention, and 131i uptake were normal. however, elevated basal tsh levels and exaggerated tsh responses to trh which normalized during the administration of thyroid extract suggested reduced thyroidal reserve. despite these finding the 131i uptake increased after exogeneous tsh, and the t3 level rose after trh. basal testost ...	1980	6773975
binding of thyroid hormone by human erythrocyte cytosol proteins.	gel filtration (g-100, 0.01 m tris, ph 7.4) of post-100,000 x g supernatant from lysate of washed human erythrocytes (rbc) revealed 3 fractions (r-1, r-2, r-3) which bound labeled t3 and t4. major peak r-2 emerged with the mehoglobin fraction (a560 nm) and binding by this fraction was partially dissociable; the dissociable site bound d-t4, but not tetraidothyroacetic acid or reverse t3. non-dissociable binding characterized peaks r-1 and r-3. r-1, r-2, and r-3 were pronase-digestible and r-1 bin ...	1980	6774869
the influence of long-term low dose thyrotropin-releasing hormone infusions on serum thyrotropin and prolactin concentrations in man.	the purpose of the present study was to evaluate in man the relative thyrotroph and lactotroph response to a 48-h low dose constant trh infusion. before, during, and after the 75 ng/min trh constant infusion, serum samples were obtained every 4 h in six euthyroid ambulating male subjects for measurements of tsh, prl, t4, and t3. the tsh response, employing a specific and sensitive human tsh ria, demonstrated a significant rise from the mean basal pre-trh value of 2.35 +/- 0.64 microu/ml (+/- sem ...	1980	6774994
the effects of exercise on pubertal progression and reproductive function in girls.	to determine whether a significant energy drain during adolescence had a significant effect on puberty and normal reproductive function, 15 ballet dancers, aged 13--15 yr, who maintained a high level of physical activity from early adolescence were followed for 4.0 yr. menarche was remarkably delayed in this group, occurring at a mean of 15.4 yr, significantly different (p < 0.01) from normal controls (12.5 yr) and normal music students (12.6 yr). in 2 dancers aged 18 yr, primary amenorrhea has ...	1980	6775000
thyroxine toxicosis. a common variant of hyperthyroidism.	to determine the frequency of thyroxine (t4) toxicosis, we calculated a free triiodothyronine index (ft3i) on 124 patients who displayed elevations of the free t4 index (ft4i) on initial screening. if the clinical presentation was not characteristic of hyperthyroidism or if the ft3i was not elevated, a thyrotropin-releasing hormone (trh) test was performed. of 83 hyperthryoid patients, 70 displayed elevations of both the ft4 and ft3 indices. thirteen patients, however, had elevations of the ft4i ...	1980	6775100
[normal values for circulating thyroid hormones, t3 uptake and thyrotropin before and after trh. radioimmunoassay determinations on 182 euthyroid children (author's transl)].	measurements of t4, t3, rt3, t3u, and tsh (before and after trh stimulation) were performed by ria. 182 children, apparently euthyroid, age 2/12--14 years, were investigated in a cross sectional study. free t4ria and free t3ria indices and the ratio rt3/t3 and t4/t3 were calculated. statistical analysis showed the following results: 1. geometric mean serum concentrations of t4, t3, rt3 and tsh (basal) show no age related differences; t4 showed a not significant negative slope with age.--2. the r ...	1980	6775189
antistreptococcal antibodies, rheumatoid factors and haptoglobin in apes and monkeys.	sera of 157 baboons (papio hamadryas and p. anubis) 21 chimpanzees (pan troglodytes) five orang utans (pongo pygmaeus) three mountain gorillas (gorilla gorilla beringei) and three gibbons (hylobates lar lar) were examined for the content of antistreptolysin o, antidesoxyribonuclease b, for the presence of rheumatoid factors as well as for the level and type of haptoglobin. the mean antistreptolysin o titer (ast) in baboons was 106 asu +/- 18 in dextransulfate absorbed sera ("real" ast) and 182 a ...	1980	6775442
hyperfunctioning thyroid nodules in children.	we studied two cases of hyperfunctioning thyroid nodules in children. a 9-year-old girl and an 11-year-old girl had thyroid masses in otherwise nonpalpable thyroid glands. scintiscan showed hyperfunctioning thyroid nodules. the former patient had elevated values for t4 and t3, and plasma thyrotropin (tsh) level failed to respond to stimulation with thyrotropin releasing hormone (trh), whereas the latter patient had normal values for t4, and t3 and plasma tsh response to trh was normal. after the ...	1980	6775526
relationship of serum t4 and t3 to tsh in primary hypothyroidism.	the relationship of serum thyroxine (t4) and 3,5,3'-triiodothyronine (t3) to the thyroid-stimulating hormone (tsh) response to protirelin was studied in 34 hypothyroid patients on a stable maintenance dose of oral levo-thyroxine. release of tsh from the anterior pituitary, as measured by basal tsh and the tsh response to protirelin, correlated better with serum t4 than serum t3. our study supports the concept that, in treated hypothyroid patients, serum t4 has a greater relative importance in me ...	1980	6775609
dose response of squamous cell carcinomas of the upper respiratory and digestive tracts.	dose-response curves have been computed for 5 tumour sites of the head and neck region, using 2 regression models. in agreement with clinical observation, a significant increase has been found from moderate to high control rates with modest dose increases for exophytic tumours (t1 + t2 retromolar trigone-anterior faucial pillar and t2 + t3 supraglottic larynx). further significant increases in local control after modest dose increases are not obtained when the control rate is already high and th ...	1980	6775658
bacillus megaterium sporal peptidoglycan synthesis studied by high-resolution autoradiography.	cells of a dap- lys- mutant strain of bacillus megaterium were pulse labeled with [3h]diaminopimelic acid at different times of growth and sporulation. they were processed for radioactivity measurements and high-resolution autoradiography either just after the pulse or after a chase in a nonradioactive medium until refractile forespores started to appear at time (t)4,5. in the pulse-labeled cells, autoradiographs and radioactivity measurements showed that the radioactivity incorporated during a ...	1980	6776097
hepatic extraction and renal production of 3,3'-diiodothyronine and 3',5'-diiodothyronine in man.	the sequential deiodination of thyroxine (t4) gives rise to several iodothyronine analogs including 3,3'-diiodothyronine (3,3'-t2) and 3',5'-diiodothyronine (3',5'-t2). in vitro animal studies suggest that the liver and the kidneys are the main sites of both formation and degradation of 3,3'-t2 and 3',5'-t2. to determine the metabolism of 3,3'-t2 and 3',5'-t2 in human liver and kidneys plasma samples were obtained from (a) a brachial artery and a hepatic vein in 20 normal subjects, and from (b) ...	1980	6776146
hyperthyroidism with normal serum total t4, t3, and thyroid binding protein concentration.	a 60-year-old woman, free of other disease, with severe clinical hyperthyroidism had normal total serum t4 with elevated free t4 concentration and t3 resin uptake characteristic of hyperthyroidism, with thyroid binding globulin (tbg) deficiency. however, serum tbg concentration as determined by immunoassay was normal, suggesting that the biochemical defect affecting the t4 binding sites did not affect is antigenicity. her total and free serum t3 levels were low-normal with a markedly elevated re ...	1980	6776158
effects of chronic heroin addiction on pituitary-thyroid function in man.	pituitary-thyroid function has been studied in heroin addicts. data have been obtained in 10 male addicts, aged 18-24 years, with histories of addiction to heroin alone lasting from 8 months to 4 years, and in 9 controls matched for sex and age. basal plasma levels of tsh, t4 and t3 were measured. a trh stimulation test was done, injecting 500 micrograms of trh iv as a bolus and assaying tsh levels before the injection and at 30 min intervals up to 2 h afterwards. the results revealed no differe ...	1980	6776179
thyroid state and mitochondrial population during maturation and ageing.	the authors have determined the serum thyroid hormone levels [total and free triiodothyronine (t3), thyroxine (t4)], the liver t3 content, the mitochondrial alpha-glycerophosphate dehydrogenase activity (alpha-gpdh) and the number of mitochondria per cell in the rat from birth to old age. the serum levels of both thyroid hormones are modified with maturation and ageing in an essentially similar way: they increase from low values at birth to remarkably high values around 12 days, and then remain ...	1980	6776181
effects of tri-iodothyronine, thyroxine and isopropyl-di-iodothyronine on thyroid-stimulating hormone in serum and pituitary gland and on pituitary concentrations of prolactin, growth hormone, luteinizing hormone and follicle-stimulating hormone in hypothyroid rats.	replacement of the 3'-halogen of the tri-iodothyronine (t3) molecule by a propyl-group produces a thyromimetic analogue, 3'-isopropyl-3,5-di-iodo-l-thyronine (t2ipr), with high biological potency. a serum thyroid-stimulating hormone (tsh) suppression test with one single intraperitoneal injection of 3 or 30 nm-t3 or t2ipr or with 30 or 300 nm-thyroxine (t4) per kg body weight was performed on 56 adult male lewis rats which were maintained for 3 weeks on an iodine-deficient diet containing 0.2% 6 ...	1980	6776220
thyroid function studies. test strategies and interpretation of results.	biochemical tests for detection of thyroid dysfunction in persons without underlying systemic illness are highly effective. in fact, one or more of the following tests are sufficiently diagnostic to allow initiation of appropriate treatment: thyroxine (t4), free t4 index (ft4-i), thyrotropin (tsh), and triiodothyronine (t3). only rarely is it necessary to measure free t4 concentration or perform a thyrotropin-releasing hormone (trh) stimulation test. patients with underlying illnesses, on the ot ...	1980	6776511
endocrine studies with mazindol in obese patients.	detailed endocrine studies were carried out in 95 hospitalized obese patients during their treatment with diet and the tricyclic anoretic mazindol. the results obtained after 1 week or more of mazindol (2 mg or occasionally 4 mg/day) administration were compared with the results after placebo and with the initial pre-treatment values. there were no significant changes in the following parameters: fsh, lh, testosterone, renin, angiotensin ii, growth hormone (gh) levels during insulin tolerance te ...	1980	6776543
thyroid function in thalassaemia major.	serum concentrations of t4, t3, rt3, and tsh were measured by radioimmunoassay in 45 patients suffering from beta-thalassaemia. a trh stimulation test was performed and the binding capacity of tbg and tbpa for t3 and t4 measured by reverse flow zone electrophoresis in a group of these patients. mean t4 serum concentration was lower in thalassaemic patients than controls; t3, rt3, tsh levels, and the pituitary response to trh were normal. tbpa binding capacity for thyroxine was greatly decreased, ...	1980	6776904
comparison of time-dependent and dose-dependent inhibitory activity of t3 and t4 on tsh release after trh injection in rats.		1980	6777286
bacteriophage infection interferes with guanosine 3'-diphosphate-5'-diphosphate accumulation induced by energy and nitrogen starvation in the cyanobacterium anacystis nidulans.	anacystis nidulans accumulates large amounts of guanosine 3'-diphosphate-5'-diphosphate (ppgpp) upon nutritional or energy starvation induced by light-to-dark shift, treatment with carbonylcyanide-m-chlorophenylhydrazone (an uncoupler), or treatment with l-methionine-dl-sulfoximine (an inducer of nitrogen starvation). in contrast to healthy a. nidulans cells, those infected by as-1 cyanophage do not respond with ppgpp accumulation when starved after about one-third of the complete infection cyc ...	1980	6777368
pituitary resistance to thyroid hormone in cystinosis.	eleven children with nephropathic cystinosis without clinical features of hyperthyroidism or hypothyroidism had elevated serum levels of immunoreactive tsh. the mean tsh level (+/- se) was 37.4 +/- 12.3 microm/ml. serial determinations of thyroid function during 1 yr were: mean (+/- se) serum t4, 10.8 +/- 0.7 microgram/dl; free t4, 2.1 +/- 0.2 ng/dl; and t3, 239 +/- 6 ng/dl. after 500 microgram trh iv, the peak tsh level exceeded 100 microu/ml in 6 of 10 patients, whereas t3 responses were varia ...	1980	6777391
effects of dextrothyroxine on the pituitary-thyroid axis in hypercholesterolemic children and goitrous adults.	to evaluate the effects of dextrothyroxine (d-t4) on the pituitary-thyroid axis, we have measured the secretion of tsh in response to trh in six goitrous adults and six euthyroid children with familial hypercholesterolemia. since the effects of thyroid hormones appear to be mediated by specific nuclear receptors, the binding affinity of d-t4 was also studied. in both groups of subjects, d-t4 completely abolished the expected trh stimulation of tsh and t3 secretion. the in vitro binding of d-t4 ...	1980	6777394
recognition of local nucleotide conformation in contrast to sequence by a rrna processing endonuclease.	rnase m5 of bacillus subtilis cleaves twice in a double-helical region of a 179-nucleotide precursor of 5s rrna to yield mature 5s rrna (116 nucleotides) plus fragments (21 and 42 nucleotides) derived from both termini. previous experiments had shown that the major recognition elements for the highly specific rnase m5 are in the mature domain of the precursor. however, one precursor residue, a g adjacent to the 5' cleavage site, significantly enhances the rate of its own cleavage as well as that ...	1980	6777771
pituitary-thyroid function in patients with breast cancer.	the present studies were carried out to clarify the relationship between thyroid function and breast diseases. neither the mean value of radioiodine uptake rate nor thyroxin (t4) level was significantly different in the patients with benign breast diseases and those with breast cancer or in localized and metastasized cancer groups. as these results were obtained by conventional studies, it became necessary to investigate the dynamic changes of pituitary and thyroid hormones after thyrotrophin re ...	1980	6777905
[hypothalamo-hypophyseal-thyroid axis in recruits and soldiers with male hypogonadism].	the functional state of hypothalamus-hypophyseal-thyroid axis was investigated in 157 recruits and soldiers, aged from 17 to 26 with different forms of male hypogonadism: system of kleinferter, male turner, primary hypogonadism with recognized and unrecognized etiology, cryptorchism, anorchia, secondary hypogonadism and late puberty. serum base level of tsh, t3, t4 were radioimmunologically determined as well as t3 binding with serum proteins. single intravenous stimulation was performed with 20 ...	1980	6777988
effect of diphenylhydantoin on hypothalamic-pituitary-thyroid function in the rat.	chronic diphenylhydantoin (dph) administration (5 mg x 100 g body wt-1 x day-1) to the normal rat is associated with a decrease in the serum thyroxine (t4) and triiodothyronine (t3) concentrations without an appropriate rise in the serum thyrotropin (tsh) concentration, suggesting a possible direct effect of dph on tsh secretion. to further study this possibility, dph was administered chronically to thyroidectomized, hypothyroid rats. in the hypothyroid rats treated chronically with dph, serum t ...	1980	6778221
solid phase radioimmunoassays (spria) using antibodies covalently bound to controlled pore glass (cpg).	covalent coupling of antibody molecules has found broad application in the area of radioimmunoassay (ria). a complete set of convenient thyroid assays has been developed employing both conventional and some new ria techniques. these assays offer the clinician rapid and precise data quickly so that appropriate diagnosis can be speedily accomplished. some of the techniques presented could only have been done with this technology. details of assays for t4, t3, tbg, tsh, and ft4 are presented in thi ...	1980	6778366
performance of bacteria filters.	several kinds and brands of bacteria filters are commercially available for use in anesthesia and respiratory therapy applications. clinical experience of high airflow resistance, ruptured media, failure to retain visible dust particles, and lack of consistent performance statements or warranties by manufacturers about their bacteria filters prompted a study of the performance of 13 different filters. the filters were challenged by mineral oil droplets, serratia marcescens and excherichia coli b ...	1980	10315105
visualization of immunosorption by scanning electron microscopy.	immunosorption has become a very important biochemical and serological tool and it is the purpose of this paper to visualize this process qualitatively using the scanning electron microscope. different carriers (i.e. cnbr activated cellulose and sepharose, glutaraldehyde treated acrylamide-agarose beads magnogel, and polystyrene cover slips) were coated with different antibodies and incubated with their homologous antigens such as pneumococci, ferritin, polymeric salmonella flagellin and mechani ...	1980	15615060
silicates as nonspecific adsorbents of bacteriophage: a model for purification of water from viruses.	amorphous silicates having small particles with a large surface area were found to have high nonspecific adsorption capacity. investigations with escherichia coli t4 bacteriophage have shown adsorption capacity of up to 99.9% on different tested silicates. optimal conditions for the adsorption of t4 bacteriophage on various commercially available silicates were determined. a continuous-flow adsorption of t4 bacteriophage on silicates appears to be a model system for the removal of viruses from p ...	1980	16345491
rna involvement in t4 dna synthesis in toluene-treated cells.	in t4-infected cells made permeable with toluene, pulses with [(alpha-32p deoxyribonucleoside triphosphates demonstrated covalent linkage of rna to dna of the okazaki fragments. analysis of the transfer of the 32p label to the 2'(3') ribonucleoside monophosphates indicated that the 3'-end of the rna primer is heterogeneous. the most frequently encountered ribonucleotide was rcmp, but also transfer to rump, ramp and rgmp occurred at different frequencies. in contrast, no heterogeneity was observe ...	1980	17941178
kinetics of the t4 gene 32 protein-single-stranded nucleic acid interaction.		1980	19431394
[association between a meningioma and an occult thyroid carcinoma in congenital hypothyroidism.]	the presence of a thyroid carcinoma and a defect in thyroid hormone synthesis in a 27 year ols female is reported. when she was 13 years old a clinical diagnosis of hypothyroid cretinism was made, based on a defect of iodide organification which was demostrated by thiocyanate test showing a discharge of 55 % of 131i at 45 minutes. she intermittently received thyroid hormone until she died. few days before dying she had normal values of t3, t4 and tsh. her parents are related and she had a 35 yea ...	1980	22167704
thyroid hormones in alcoholic liver disease. effect of treatment with 6-n-propylthiouracil.	the relationship between alcoholic liver disease and circulating thyroid hormones was investigated in 124 hospitalized patients treated with placebo or propylthiouracil (ptu) for a maximum of 46 days in a double-blind study. serum triiodothyronine (t3) levels on admission were significantly (p less than 10(-6) and inversely correlated with the severity of alcoholic liver disease. after hospitalization, changes in t3-levels in patients with low admission t3 significantly correlated (p less than 0 ...	1979	758132
[alteration of tsh secretion in children with goitre after short-term treatment with potassium iodide (author's transl)].	300 microgram of potassium iodide daily were administered over a period of two weeks to 12 children with goitre. basal tsh concentrations, maximal tsh increase after trh and integrated tsh secretion after trh were significantly decreased in this group whereas the increase of total thyroxine (t4-ria) did not reach significance. it appears that in the development of iodine deficiency goitre in children a sensitive feed-back system is predominant. early administration of iodine or even better gener ...	1979	759165
nutritional evaluation of low-glucosinolate rapeseed meals obtained by various processes.	defatted meals were prepared from two new varieties of rapeseed, the 'high-glucosinolate' variety, brassica napus, lesira, and the 'low-glucosinolate' variety, brassica napus, erglu, by each of the following processes: (1) defatting of the ground seed with hexane; (2) extraction of the myrosinase-deactivated ground seed with 70% aqueous acetone for the removal of glucosinolates followed by defatting with pure acetone, and (3) autolysis of the ground seed for the decomposition of glucosinolates f ...	1979	759989
changes in thyroid hormones by treatment with aspirin and prednisolone in subacute thyroiditis with hyperthyroidism.	thyroid functions were studied in 11 patients with subacute thyroiditis accompanied by signs and symptoms of hyperthyroidism, and were compared with 13 patients with untreated thyrotoxicosis in which serum t4 was elevated to the identical level. serum t3 was also elevated in subacute thyroiditis but to a significantly lower extent than in thyrotoxicosis. therefore the ratio of t4/t3 was significantly higher in subacute thyroiditis than in thyrotoxicosis. although duration of thyroid swelling was ...	1979	760253
a methodological study in measuring t3 and t4 concentration in red and white skeletal muscle and plasma of euthyroid rats.	t3 and t4 concentrations were determined in plasma and red and white skeletal muscle of the rat. because of the small tissue samples (+/- 300 mg), the ultra-sensitive wick radioimmunoassay (ria) for serum was adapted for determination in ethanol extracts. the dilution curves of the plasma and tissue extracts showed excellent parallelism with the standard curves for both t3 and t4. the mean t4 level found in female rats (n = 6) was 22.6 +/- 5.2 ng/ml in plasma and did not differ significantly bet ...	1979	760360
isolated high serum triiodothyronine levels. clinical relevance after sodium iodide i 131 therapy.	ten of 25 thyrotoxic patients treated with sodium iodine i 131 had thyroid function test results that indicated a high triiodothyronine (t3) level, with normal thyroxine (t4) and thyroid-stimulating hormone (tsh) levels, within 15 months of treatment. this pattern was usually transitional and lasted a variable time, eventually leading to euthyroidism, hyperthyroidism, or hypothyroidism. it is concluded that an isolated elevation of t3 levels after radioactive iodine therapy can be associated wit ...	1979	760681
correction for the presence of cross-reactants in saturation assays: application to thyroxine cross-reactivity in 3,3',5'-(reverse)-triiodothyronine radioimmunoassay.	cross-reaction of anti-3,3',5'-triiodothyronine (rt3) antisera with thyroxine has proved problematical in the development of radioimmunoassays for rt3. results of experimental work with two antisera with differing specificities are presented which illustrate certain aspects of cross-reacting assay systems. the mathematical theory of a single binding-site, two ligand assay is discussed and extended by use of a multiple binding-site computer model to a two binding-site, two ligand system. it is su ...	1979	761407
purification of t4 rna ligase by 2', 5'-adp sepharose chromatography.		1979	761619