Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| permissive role of l-thyroxine in induction of pancreatic amylase by cortisol in neonatal rats. | the effect of l-thyroxine (t4) on amylase activity in the developing rat pancreas has been investigated. administration of t4 (0.2 microgram/g body wt) alone to intact rats on days 5-10 after birth did not induce pancreatic amylase but the enzyme was induced significantly by daily injection of cortisol (10 microgram/g body wt) alone into intact rats over the same period. in thyroidectomized, adrenalectomized rats pancreatic amylase was not induced by the injection of cortisol alone but it was in ... | 1980 | 6159441 |
| l-thyroxine, cortisol and diet affect the level of amylase in the parotid gland of developing rats. | the effects of cortisol (10 microgram/g body weight) and l-thyroxine (t4; 0.2 microgram/g body weight) on the activity of parotid gland amylase in young rats were investigated. administration of cortisol or t4 for 5 consecutive days from day 5 after birth caused the precocious appearance of amylase, t4 having almost twice the effect of cortisol. cortisol and t4 did not have synergistic effects. in thyroidectomized-adrenalectomized rats, t4 increased amylase activity but cortisol did not. the inc ... | 1980 | 6159442 |
| 3' terminal labelling of rna of rna with beta-32p-pyrophosphate group and its application to the sequence analysis of 5s rna from streptomyces griseus. | nucleotide pyrophosphate transferase isolated from streptomyces griseus is used to transfer pyrophosphate group from gamma-32p-atp to the 3'-oh of trna, generating a strictly terminal label at its 3' end. using yeast trnaphe as model compound, it is demonstrated that the labelled molecule is suitable for rapid gel sequencing by both enzymatic and chemical methods. rna molecules terminated by pyrimidine nucleoside are poor pyrophosphate acceptors. to label rnas of this kind, first guanosine 5'-ph ... | 1980 | 6159585 |
| the binding of t4 gene 32 protein to ms2 virus rna and transfer rna. | fluorescence titrations, absorption spectroscopy and stopped-flow techniques were used to study the interaction of t4 coded 32-protein (p 32) with ms2 rna and total trna from e. coli under different ionic conditions. it is shown that the amount of ms2 rna and trna secondary structure melted by p 32 varies markedly and reversibly within a range of ionic conditions under which the binding constant of p 32 to single-stranded nucleic acids unable to form stable hairpins remains higher than 10(8) m-1 ... | 1980 | 6159594 |
| [neuropeptides in regulation]. | 1. the thyroid gland is regulated on nerval and neurohormonal way by the hypothalamus, in which case the hypothalamus is regarded as one of the most important limbs in the chain of the limbic system. 2. the thyreotropin releasing hormone, a tripeptide, is characterized in its double function as releaser and as neuroregulator. as a releaser it controls the function of the thyroid gland by releasing the thyroid gland stimulating hormone of the pituitary gland and by guaranteeing the adaptation to ... | 1980 | 6159744 |
| inhibitory effects of desmethylimipramine on the action of thyroxine on cardiac beta-receptors and myocardial phosphorylase. | the influence of chronic administration of desmethylimipramine (dmi) on the actions of thyroxine (t4) on rat heart was studied. administration od dmi (10 mg/kg, i.p., twice daily) for 7 days did not alter the cardiac beta-adrenergic receptor density or the affinity for (-)[3h]dihydroalprenolol (3h-dha) but diminished the activation of myocardial phosphorylase produced by isoproterenol. chronic injections of t4 (500 microgram/rat) for 5 days caused an increase in the number of beta-adrenergic rec ... | 1980 | 6160335 |
| [genetic deficiency of thyroxin-binding protein in 4 members of a family (author's transl)]. | four members of the same family (2 male and 2 female) with normal thyroid function were found to have decreased total t3 and total t4, elevated response to the t3 test and low free thyroxin index. such biological changes result from a deficiency in carrier protein and should be distinguished from nearly similar abnormalities observed in low t3 syndrome and hypothyroidism. whilst gonosomal transmission through the x chromosome of thyroxin-binding protein deficiency seems well established, our fin ... | 1980 | 6160453 |
| radioimmunoassay of thyroxine-binding globulin: evaluation of a kit and diagnostic application. | serum thyroxine-binding globulin (tbg) was measured by using a radioimmunoassay method. the within-batch coefficient of variation (cv) of the assay was 5% or less at different tbg concentrations with a between-batch variation of less than 10%. the mean tbg concentration in hypothyroidism (28.5 +/- 6.7 mg/l) was significantly higher, and that in hyperthyroidism (22.3 +/- 3.5 mg/l) was significantly lower, than the euthyroid mean (25.4 +/- 6.9 mg/l). acutely ill patients suffering from various non ... | 1980 | 6160801 |
| combined bleomycin and radiotherapy in oral cancer. | a clinical trial comparing bleomycin (blm) plus radiation against radiation alone is reported. one hundred and fifty-seven previously untreated t3 and t4 and n0, n1 or n2 buccal squamous cell carcinomas were entered. eighty-four of these received the combined therapy and 73 were controls. cobalt-60 teletherapy using two opposing fields was employed. blm was administered intra-arterially in 42 patients, intravenously in 22 patients and intramuscularly in 20 patients. the 73 controls received phys ... | 1980 | 6162599 |
| relations between serum levels of tsh, tbg, t4, t3, rt3 and various histologically classified chronic liver diseases. | serum levels of tsh, thyroxine-binding globulin (tbg), t4, t3 and reverse t3 (rt3) were measured in 36 patients with fatty liver disease, 11 patients wih chronic persistent hepatitis, 17 patients with chronic active hepatitis, and 29 patients with liver cirrhosis. tbg was significantly above normal levels in both groups of chronic hepatitis, the slight concomitant t4 and t3 increase was significant only for t4 in chronic persistent hepatitis. a significant decrease in t4 and t3 concentration was ... | 1980 | 6162877 |
| clinical and laboratory evaluation of four methods of assessing free thyroxine status in thyroid clinic patients. | the clinical value of four laboratory methods of assessing free thyroxine status was compared in 82 consecutive patients newly referred to a thyroid clinic with suspected thyroid dysfunction. the methods of determining free thyroxine used were: (1) free thyroxine index using a thyroid hormone uptake test (fti (thut)); (2) a free thyroxine index using thyroxine binding globulin (fti (tbg)); (3) a kinetic radioimmunoassay (immophase); and (4) an equilibrium dialysis method. the definitive thyroid ... | 1980 | 6163391 |
| treatment of obesity with triiodothyronine and a very-low-calorie liquid formula diet. | in a double-blind trial physiological doses (60 microgram/day) of triiodothyronine (t3) were compared with placebo in a group of obese patients on a 320 kcal/day (1.34 mj) liquid formula diet. t3-treated patients had a significantly greater weight-loss by the 12th week. analysis of serum levels of t3 and thyroxine (t4) showed that those patients in the placebo group whose weight-loss tended to level off at a plateau towards the end of the trial also had the lowest t3 levels and resting metabolic ... | 1980 | 6101677 |
| prevention of postoperative lymphopenia and granulocytosis by epidural analgesia. | blood leucocyte-counts, cortisol, and glucose were measured in twelve healthy premenopausal women undergoing elective abdominal hysterectomy during either general anaesthesia (six women) or epidural analgesia (t4 to s5) (six women). surgery during general anaesthesia caused significant lymphopenia 6 and 9 h after skin incision and significantly increased granulocyte-counts 6, 9, and 24 h after skin incision. epidural analgesia prevented lymphopenia and reduced granulocytosis to about 40% of that ... | 1980 | 6101739 |
| inhibition by propranolol of 3,5,3'-triiodothyronine formation from thyroxine in isolated rat renal tubules: an effect independent of beta-adrenergic blockade. | recent studies in man have shown a decrease in serum l-t3 (t3) levels in subjects treated with dl-propranolol, but the mechanism of this effect is unknown. isolated rat renal tubules were used to study the effect of propranolol and related drugs on the formation of t3 from l-t4 (t4). racemic (dl) propranolol at 100 microm inhibited the net formation of t3 from t4 by 35% (p less than 0.01). the d- and l-isomers of propranolol were also potent in inhibiting t3 formation, but the beta-blockers aten ... | 1980 | 6102511 |
| free-thyroxine index in schizophrenic patients before and after neuroleptic treatment. | the mean values of serum thyroxine (t4), in vitro radioactive triiodothyronine uptake and free-thyroxine index (fti) in 41 drug-free schizophrenic patients did not differ significantly from those of euthyroid controls. following 6 weeks' treatment of 24 schizophrenics with chlorpromazine, trifluoperazine or clozapine, a significant decrease in serum t4 and fti was noted after chlorpromazine and clozapine, whereas after trifluoperazine only serum t4 decreased, but not fti. the questions arising f ... | 1980 | 6102744 |
| changes in thyroid function tests during phenobarbital treatment in late pregnancy. | the effect of phenobarbital treatment in late pregnancy on thyroid function tests was investigated in 16 women. ten women in late pregnancy served as controls. phenobarbital treatment for two weeks was associated with a significant decrease in serum thyroxine (t4) level and in the free thyroxine index (t3u x t4) of 18% and 16%, respectively. these changes were associated with a significant increase in serum thyrotropin concentration of 15%. gamma-glutamyl transpeptidase activity increased, and s ... | 1980 | 6103842 |
| somatostatin and the 24 h levels of serum tsh, t3, t4, and reverse t3 in normals, diabetics and patients treated for myxoedema. | 1980 | 6104408 | |
| [thyroid function in premature infants of different gestational age during the first month of life (author's transl)]. | to assess the function of the thyroid gland in premature infants of different gestational ages during the first month of life we determined simultaneously tsh, t4, t3, and rt3 serum concentrations in 116 preterm infants (gestational ages 31st to 38th week) during each of the first 30 days of life. the serum concentrations of tsh, t3, and rt3 changed significantly during this period. the tsh and rt3 values were highly increased on the first day and decreased thereafter. the t3 values, however, in ... | 1980 | 6107824 |
| lack of effect of somatostatin on thyroid gland function in graves' disease. | five women with graves' disease, 26-52 years of age, with serum concentrations of triiodothyronine (t3) 4.8-9.2 nmol/l and thyroxine (t4) 200-320 nmol/l were studied. a 26 h infusion of cyclic somatostatin (bachem), 6 mg in isotonic saline solution was administered. radioactive iodine i.v. (125i or 131i) was given immediately after the start of this infusion. serum t3, t4 and conversion rate (cr% = pbri: total ri x 100) were determined four times during the infusion, then daily for a week. the s ... | 1980 | 6108171 |
| postpartum recurrence of hyperthyroidism and changes of thyroid-stimulating immunoglobulins in graves' disease. | the changes of circulating thyroid-stimulating antibody (tsab) in three patients with graves' disease with relapsed hyperthyroidism after delivery were examined. all three patients were in clinical remission before and during pregnancy, but hyperthyroidism, with high radioactive iodine uptake, recurred 3--5 months post partum. tsab activity, measured serially, was not detected at the time of the postpartum relapse, although it was detectable during pregnancy and in the euthyroid state after ther ... | 1980 | 6108332 |
| kinetics of imp:nad+ oxidoreductase in serum of normal iraqi individuals. | the general kinetic parameters of imp:nad+ oxidoreductase, were investigated at 37 degrees c in normal human serum. these included substrate - velocity relationship, km (imp), km (nad), as well as the effect of temperature and ph on the kinetics of the reaction. optimum conditions of imp, nad+ oxidoreductase in serum of normal iraqi individuals at 37 degrees c were investigated. the optimum imp concentration was found to be 0.6166 mm and the optimum nad+ concentration was 6.28 mm, while the opti ... | 1980 | 6111329 |
| affinity labeling of rat liver thyroid hormone nuclear receptor. | the thyroid hormone receptor from rat liver nuclei has been covalently labeled with the n-bromoacetyl derivatives of l-thyroxine (t4) and 3,3',5-triiodo-l-thyronine (t3). displacement binding studies showed that, in the presence of 100-fold molar excess of unlabeled n-bromoacetyl-t3 or t4, binding of [125i]t3 or [125i]t4 was nearly totally inhibited. heat inactivation of the receptor (55 degrees c for 15 min) resulted in parallel losses in the binding of t3 (95%) and n-bromoacetyl-t3 (93%). thes ... | 1980 | 6261237 |
| [the effect of thyroid function on the increase of plasma cyclic amp following glucagon injection (author's transl)]. | the increase in plasma cyclic adenosine-3':5'-monophosphate (camp) was measured after intravenous injection of 1 mg of glucagon in 26 normal subjects, 36 patients with hyperthyroidism, 35 patients with hypothyroidism and 24 patients with euthyroid goitre. while patients with euthyroid goitre responded normally, the plasma cyclic amp response in patients with hyperthyroidism was considerably increased and in those with hypothyroidism decreased. 4 patients with cirrhosis of the liver had reduced r ... | 1980 | 6259272 |
| molecular cloning of the t4 genome; organization and expression of the frd--dna ligase region. | 1980 | 6258011 | |
| a restriction map of the bacteriophage t4 genome. | we report a detailed restriction map of the bacteriophage t4 genome and the alignment of this map with the genetic map. the sites cut by the enzymes bg/ii, xhoi, kpni, sali, psti, ecori and hindiii have been localized. several novel approaches including two-dimensional (double restriction) electrophoretic separations were used. | 1980 | 6258018 |
| protein conformation wheels: cytochromes and lysozymes. | conformation wheels, directly relating to amino acid sequence to the local torsion angles in a protein molecule, are presented for cytochromes c, c2, c550, and c551 and for lysozymes from hen egg-white and t4 bacteriophage. the circular plots for the cytochrome molecules aid in visualizing the common three-dimensional folding ("cytochrome fold") observed in this family of proteins. conformation wheels for lysozymes from two different species reveal the characteristic differences in their folding ... | 1980 | 6257619 |
| thyroxine 5'-monodeiodinase activity in regenerating liver of triiodothyronine-treated rats. | the effect of triiodothyronine (t3) on hepatic thyroxine (t4) 5'-monodeiodinase and the subcellular localization of the enzyme were examined in regenerating rat liver, because it seemed likely that the effect of t3 might be accentuated during liver regeneration. five days after t3 treatment, the specific activity of the monodeiodinase in the microsomal fraction (105,000 x g pellet) of regenerating liver was increased to 207% of the control value. lineweaver-burk plots showed that the vmax for t4 ... | 1980 | 6257668 |
| physical chemical studies of the structure and function of dna binding (helix-destabilizing) proteins. | binding of proteins to dna is fundamental to the mechanisms of replication, recombination and gene expression. the specific molecular features of dna recognized by complementary features of the three-dimensional structure of the dna binding proteins are under intensive investigation. two large classes of dna binding proteins have emerged. one class includes enzymes such as the rna polymerases and restriction endonucleases and the nonenzymatic repressor proteins which recognize unique sequences p ... | 1980 | 6243083 |
| hypothalamic-pituitary evaluation in patients with galactorrhea-amenorrhea and hyperprolactinemia. | twenty-two women with galactorrhea and/or amenorrhea were evaluated. thyroxine (t4), thyroid-stimulating hormone (tsh), and visual fields were normal. hyperprolactinemia (22 to 440 ng/ml) was present in 19 patients. evidence of tumor was found in 73.7% of the woemn, whereas 26.3% were classified as functional. three patients with empty sella syndrome showed normal prolactin levels (12.2 to 18.5 ng/ml). there was a significant negative correlation between prolactin levels and follicle-stimulating ... | 1980 | 6243406 |
| binding of thyroid hormones in vivo by hepatic nuclei of rana catesbeiana tadpoles. | thyroid hormone is essential for amphibian metamorphosis, and tadpoles develop responsiveness to exogenous t4 and t3 during the premetamorphic stage of development. the present studies were performed to investigate the receptors concerned with the initiation of this response. premetamorphic tadpoles (stages vii-xv of taylor and kollros) were injected ip with [125i]t3 or [125i]t4 (0.001-10 nmol/tadpole). twenty-four hours later, liver and serum were obtained, and organic 125i in liver nuclei and ... | 1980 | 6243554 |
| rna priming of dna replication by bacteriophage t4 proteins. | bacteriophage t4 dna replication proteins have been shown previously to require ribonucleoside triphosphates to initiator new dna chains on unprimed single-stranded dna templates in vitro. this dna synthesis requires a protein controlled by t4 gene 61, as well as the t4 gene 41, 43 (dna polymerase), 44, 45, and 62 proteins, and is stimulated by the gene 32 (helix-destabilizing) protein. in this paper, the nature of the rna primers involved in dna synthesis by the t4 proteins has been determined, ... | 1980 | 6243660 |
| binding of endogenous iodothyronines to isolated liver cell nuclei. | the metabolic role of a number of the metabolites of t4 is unknown. hence, these iodothyronines, now known to be present in human serum, were tested for their ability to displace [125i]t3 from specific binding sites in isolated pig liver nuclei. compared with t3 (1.0), the molar inhibition ratios of the analogs tested were: triiodothyroacetic acid, 4.4; t4 6.2; 3.3'-diiodothyronine, 56; 3,5-diiodothyronine, 245; rt3, 264; and 3',5'-diiodothyronine, 60,000. in isolated pig liver nuclei, the ka fo ... | 1980 | 6244141 |
| [bone metastases of prostatic neoplasms with reference to the histological type and local tumor stage]. | in prostatic cancer the frequency of osseous metastases - detected earliest by bone scintigraphy - amounts to 21.8% at the time of histological verification of the primary tumour. this frequency depends on the histological type of the prostatic carcinoma and yields 10.5% in uniform adenocarcinomas and 40.4% in pluriform carcinomas. the rate of metastases in the uniform adenocarcinomas rises with increasing histological dedifferentiation. the percentage of prostatic carcinomas with pluriform patt ... | 1980 | 6244617 |
| [strategy for laboratory tests of thyroid function (author's transl)]. | clinical findings suggesting hyperthyroidism should be confirmed by the following basic tests of thyroid function: serum t3 and t4, and ift4. in the case where there is contradiction between the t3 and t4 values, hyperthyroidism may be confirm by the absence of effect of trh on serum tsh level. clinical findings suggesting hypothyroidism should be confirmed by the following basic tests: serum t3 and t4, ift4, and serum tsh which also will be greatly helpful in the differentiation of primary or s ... | 1980 | 6244635 |
| effect of vitamin a on the hypothalamo-pituitary-thyroid axis. | this study reports the effects of the administration of pharmacologic doses of vitamin a on multiple parameters of thyroid function. vitamin a decreased total t4 and t3 levels. with vitamin a treatment, there was a marked increase in the percentage dialyzable t3 and t4 both in vivo and in vitro. the serum-free t3 and t4 levels as measured by dialysis were on the whole normal in vitamin a-treated rats. following thyroidectomy, the total t4 levels were still decreased, suggesting that vitamin a pr ... | 1980 | 6244741 |
| type ii dna topoisomerases: enzymes that can unknot a topologically knotted dna molecule via a reversible double-strand break. | the t4 dna topoisomerase is a recently discovered multisubunit protein that appears to have an essential role in the initiation of t4 bacteriophage dnd replication. treatment of double-stranded circular dna with large amounts of this topoisomerase in the absence of atp yields new dna species which are knotted topological isomers of the double-stranded dna circle. these knotted dna circles, whether covalently closed or nicked, are converted to unknotted circles by treatment with trace amounts of ... | 1980 | 6244895 |
| release of thyrotropin receptor from thyroid plasma membranes: effect of hydrocortisone, propranolol, and adenosine 3',5'-monophosphate. | soluble tsh receptors were released into the medium when bovine thyroid plasma membranes were incubated in 0.01 m tris-hcl, ph 7.5, at 0 or 20 c. this is a conventional hypotonic medium used in binding assays. the characteristics of binding of bovine [125i]tsh to the released tsh receptor were almost the same as those of binding to tsh receptors solubilized by lithium 3,5-diiodosalicylate or to the original plasma membrane. released tsh receptor had two binding sites with ka values of 0.7 x 10(1 ... | 1980 | 6244931 |
| silicates: non-specific adsorbents in purification of water from viruses. | amorphous silicates having small particles with large surface area, were found to have high non-specific adsorption capacity. escherichia coli t4 bacteriophage was used as a model in this research. investigations have shown an adsorption capacity of up to 99% on different tested commercially available silicates. optimal conditions for the adsorption of t4 bacteriophage on various silicates were determined. amorphous silicates may be used in purification of water from viral contaminants. a silica ... | 1980 | 6245005 |
| purification and properties of deoxyribonucleic acid binding factor isolated from the surface of streptococcus sanguis cells. | deoxyribonucleic acid (dna) binding factor (bf) was found in surface fluids from competent and noncompetent cells of streptococcus sanguis strains challis, wicky, and blackburn. fluids from noncompetent cells exhibited about 10% bf activity compared with extracts from competent cells. bf from competent wicky cells was purified to homogeneity by electrophoresis and immunodiffusion. purified bf preparations exhibited slight endonucleolytic activity, directed mainly against single-stranded dna. nuc ... | 1980 | 6245057 |
| regulation of cyclic nucleotide and prostaglandin formation in normal human thyroid tissue and in autonomous nodules. | we have investigated the regulation of the human throid gland based on controls discovered in the dog thyroid gland. tsh and thyroid-stimulating immunoglobulin enhanced camp accumulation, which supports the validity of the sutherland model for the action of tsh on the human thyroid. iodide inhibited tsh- and thyroid-stimulating immunoglobulin-activated camp accumulation and this effect was reduced by methimazole, showing that, in this tissue, iodide, through an oxidized derivative, depresses the ... | 1980 | 6245102 |
| adp ribosylation of escherichia coli rna polymerase is nonessential for bacteriophage t4 development. | the bacteriophage t4-induced alt and mod gene products covalently add adp-ribose to the escherichia coli rna polymerase alpha polypeptides; phage carrying either an alt or a mod mutation are viable. a genetic cross between t4alt and t4mod phages yielded alt mod recombinant progeny which could not adp ribosylate rna polymerase at all, yet grew apparently normally. thus, adp ribosylation of rna polymerase appeared to be nonessential for t4 development (at least in e. coli b/r and e. coli cr63), ev ... | 1980 | 6245251 |
| the effect of physiological doses of thyroxine on the level of cyclic adenosine-3',5'-monophosphate in pituitary and anterior hypothalamus of male rats of different age. | the levels of camp in the pituitary and anterior hypothalamus were determined by competitive binding to protein in male rats aged 2--2.5, 6--8 and 14--18 months. the lowest level of camp both in the pituitary and anterior hypothalamus (1.05 +/- 0.17 and 0.85 +/- 0.23 pm) (per mg wet weight, respectively) was found in 6--8 month-old rats. administration of t4 (2 micrograms/100 g body weight, for 8 days) was followed by a decrease in camp level in the pituitary of 14--18 month-old animals, which w ... | 1980 | 6245861 |
| decatenation of kinetoplast dna by topoisomerases. | kinetoplast dna is the mitochondrial dna of trypanosomatids such as crithidia fasciculata. this dna is in the form of networks containing thousands of dna circles which are apparently catenated (interlocked). some topoisomerases, such as t4 phage topoisomerase and dna gyrase, catalyze a decatenation of the networks to form individual covalently closed circles. | 1980 | 6246090 |
| mechanism of t4 phage restriction by plasmid rts 1. cleavage of t4 phage dna by rts 1-specific enzyme. | 1980 | 6246113 | |
| a restriction map of the t4 transfer rna gene cluster. | 1980 | 6246243 | |
| the cloning of a t4 transfer rna gene cluster. | 1980 | 6246244 | |
| the generation and analysis of clones containing bacteriophage t4 dna fragments. | 1980 | 6246245 | |
| purification and properties of a pyrimidine dimer-specific endonuclease from e. coli infected with bacteriophage t4. | 1980 | 6246342 | |
| fidelity of dna replication catalysed in vitro on a natural dna template by the t4 bacteriophage multi-enzyme complex. | more than 50 copies of a phi x174 dna template can be made in 60 min in an in vitro dna replication system consisting of seven purfied replication proteins isolated from t4 bacteriophage-infected cells. by transfecting with the dna products and assaying for the reversion of specific amber mutants, the high degree of base-pairing fidelity in this system is revealed; the in vitro system is also shown to respond to the mutagenic effect of mn2+ and to display strong base-pair context effects on fide ... | 1980 | 6246450 |
| [clinical and biological patterns of hyperthyroidism in elderly patients (author's transl)]. | aetiological, clinical and biological patterns of hyperthyroidism are studied in 22 consecutive patients over the age of 65 years. in this group the frequency of toxic multinodular goiter and graves disease are grossly the same. a previous iodine administration could be a significant aetiologic factor in 8/22 patients. the clinical symptomatology is characterized by the predominance of cardiovascular disorders and by the mildness or absence of the other symptoms of thyrotoxicosis. the classical ... | 1980 | 6246589 |
| effects of thyroid hormone on utp content and uridine kinase activity of rat heart and skeletal muscle. | in rats made hyperthyroid by daily intramuscular injections of 250 microgram thyroxine (t4)/100 g body wt for 5 days, uridine kinase activity of extracts of psoas and cardiac muscle was markedly increased vmax of the enzyme was elevated with no change in the apparent km for uridine. in animals treated as above, significant increases in utp and total uracil nucleotide contents were observed in heart and skeletal muscle. twelve hours after a single intraperitoneal injection of 30 microgram/100 g b ... | 1980 | 6246811 |
| effects of coronary artery occlusion on thoracic spinal neurons receiving viscerosomatic inputs. | action potentials of single cells in the gray matter of the t2 to t4 spinal segments of chloralose-anesthetized cats were recorded during occlusion of the anterior descending branch of the left coronary artery. only cells responding both to visceral and somatic afferent inputs were examined. in 53% of the cells the discharge frequency was unaffected by coronary artery occlusion (cao). the remaining 47% could be classified by four response patterns: 1) in 18% frequency increased immediately follo ... | 1980 | 6246816 |
| pauses at positions of secondary structure during in vitro replication of single-stranded fd bacteriophage dna by t4 dna polymerase. | 1980 | 6246822 | |
| binding of t4 endonuclease v to deoxyribonucleic acid irradiated with ultraviolet light. | endonuclease v of bacteriophage t4 binds to uv-irradiated deoxyribonucleic acid (dna) but not to unirradiated dna. we have developed an assay to detect this binding, based on the retention of enzyme--dna complexes on nitrocellulose filters. the amount of complex retained, ascertained by using radioactive dna, is a measure of t4 endonuclease v activity. the assay is simple, rapid, and specific, which makes it useful for detecting t4 endonuclease v activity both in crude lysates and in purified pr ... | 1980 | 6246930 |
| thyroxine-induced changes in characteristics and activities of beta-adrenergic receptors and adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate systems in the heart may be related to reputed catecholamine supersensitivity in hyperthyroidism. | modifications in characteristics and activities of beta-adrenergic receptors and certain parameters of the cyclic nucleotide systems were observed in the hypertrophied heart of the rat chronically treated with t4. these include: 1) an increased number of beta-adrenergic receptors without a change in their affinity, as determined by binding of (-)-[3h]dihydroalprenolol to the membrane; 2) increased sensitivity and magnitude of stimulation of adenylate cyclase in homogenates by isoproterenol, with ... | 1980 | 6247145 |
| binding of thyroid hormones in serum and liver cytosol of rana catesbeiana tadpoles. | binding of thyroid hormones to proteins in tadpole serum and liver cytosol was studied using both equilibrium dialysis and dextran-coated charcoal techniques to separate bound and free hormone. measured directly by equilibrium dialysis, approximately 1% of the total t3 or t4 in serum and less than 0.5% of the total t3 and t4 in cytosol (diluted 1:5 or 1:10) were in the free form. free t3 and t4 fractions were independent of hormone concentration between 10(-10) and 10(-6) m. values for free horm ... | 1980 | 6247146 |
| solubilized nuclear thyroid hormone receptors in circulating human mononuclear cells. | in order to assess iodothyronine receptor interactions in man, we have developed a receptor assay for t3 and t4 in solubilized nuclear extracts from circulating mononuclear cells. this assay utilizes the technique of salt solubilization to isolate nuclear receptors and employs standard saturation analysis for t3 and t4 to determine maximal binding capacity (mbc) and equilibrium dissociation constants (kd). we have determined that 11 normal subjects had a mbc for t3 of 1.20 +/- 0.20 pmol/mg dna ( ... | 1980 | 6247359 |
| [new enzymatic and endocrinological aspects in two cases of werner's syndrome (author's transl)]. | results of endocrinological studies and of the investigation of intraerythrocytic glycolytic pathway carried out in two patients with werner's syndrome are presented. an increase of glyceraldehyde 3-phosphate and 3-phospho-glycerokinase has been observed, but the significance of these original findings is still unknown. an hyperinsulinism after oral glucose overload has been demonstrated too. this finding, which has been reported by other authors, probably reflects a peripheral resistance to ins ... | 1980 | 6247588 |
| [pituitary dwarfism in a german shepherd dog (author's transl)]. | pituitary dwarfism has been described in more than 20 german shepherd dogs. some cases probably were caused by persistent cysts of rathke's pouch. this is the first histopathological, immunohistochemical and endocrinological study. a 13-month-old, 7-kg, dwarf purebred german shepherd bitch with alopecia and hyperpigmentation was admitted to the clinic for euthanasia. retardation of growth was noticed when the dog was about two months old. no littermates had this condition. two subsequent breedin ... | 1980 | 6247812 |
| peripheral insensitivity to thyroid hormones in a euthyroid girl with goitre. | a 9-year-old girl was euthyroid with a small goitre, exophthalmos, scaphocephalic skull, minor sketelal abnormalities, and raised serum thyroid hormone concentrations. other members of the family did not have goitres and their thyroid hormone levels were normal. from age 3 years the patient was treated for graves's disease, but after 4 years treatment was stopped because of enlargement of the goitre. despite increased serum thyroxine (t4), free t4 (ft4), and triiodothyronine (t3), basal serum ts ... | 1980 | 6247978 |
| physical properties of the complementary t4 rna. | the complementary transcribed t4 rna after self-annealing and rnaase treatment was isolated by gel chromatography and then used for further studies. from salt-dependent rnaase resistance and melting studies it is evident that this rna represents a genuine double-stranded structure. the base content of the isolated double-stranded rna was found to be the same as total t4 mrna. sucrose gradient analysis and hydroxyapatite chromatography of t4 rna, annealed early and late rna, and of the isolated d ... | 1980 | 6248116 |
| role of thyroid hormones in the normal and glucocorticosteroid hormone-induced evolution of carbamoylphosphate synthase (ammonia) and arginase activity in perinatal rat liver. | administration of thyroid hormones causes a dose-dependent increase in carbamoylphosphate synthase (ammonia) and arginase activities in fetal rat liver but not in neonatal rat liver. simultaneous administration of thyroid and glucocorticosteroid hormones enhances enzyme accumulation still further in the fetus. when administered before birth, the relative potencies of t4 and reverse t3, compared to t3 are 20--25% and 1--20%, respectively. both before and after birth, thyroid hormones enhance dna ... | 1980 | 6248135 |
| changes of blood levels of several hormones, catecholamines, prostaglandins, electrolytes and camp in man during emotional stress. | the levels of several hormones (acth, gh, tsh, fsh, lh, parathyroid hormone--pth, insulin, thyroxine--t4, triiodothyronine--t3, cortisol, testosterone, aldosterone, renin), catecholamines (epinephrine, norepinephrine, dopamin), prostaglandins (f1 alpha, f2 alpha, a + e), electrolytes (na, k, ca, mg), camp and glucose in blood were measured before and immediately after the examination in 15 male students aged 28 to 35 years. simultaneously the blood pressure was measured and hemodynamic measures ... | 1980 | 6248318 |
| cloning dna restriction endonuclease fragments with protruding single-stranded ends. | a new method of in vitro recombination was employed to construct plasmids containing lac promoter fragments 64 bp and 144 bp long. the 64 bp hpaii-hhai fragment contains the binding site for the catabolite activator protein (cap). the hpaii-haeiii 144 bp fragment includes the binding sites for rna polymerase, the lac repressor and cap. the method utilizes the ability of t4 dna polymerase to make flush-ended dna either by filling in a recessed 3'-end or by exonucleolytic removal of a protruding 3 ... | 1980 | 6248431 |
| thyrotropin-induced increase in phosphoprotamine phosphatase activity in rat thyroid. | phosphoprotamine phosphatase activity in the rat thyroid was examined by studying the release of inorganic phosphate from [32p]-phosphorylated protamine. rats were given a normal diet supplemented or not with thyroxine (t4) (3 mg/l in 0.05% bovine serum albumin solution) or propylthiouracil (1 g/l in 1% sucrose solution) in drinking water for various periods. tsh was injected ip, 100 mu per animal. a significant increase of phosphatase specific activity-units per mg protein (3-fold) as well as u ... | 1980 | 6248588 |
| complementation of t4 phage am mutations by hybrid phages lambda-t4. | ecori fragments of the t4 cytosine-containing dna (dc-dna) were cloned in the lambda xiii vector phage carrying the only restriction site for ecori in the repressor gene of lambda. 38 genes of t4 were identified in the cloned fragments by means of marker rescue technique. all cloned early genes and some late genes of t4 were able to complement a corresponding am mutations of t4 phage when nonpermissive cells of e.coli were simultaneously infected with hybrid phages lambda-t4 and am mutants of t4 ... | 1980 | 6248735 |
| deoxyribonuclease i generates single-stranded gaps in chromatin deoxyribonucleic acid. | production of 10-base multiple dna ladder fragments during dnase i digestion of chromatin is explained by a model which does not involve site-specific nicking by the dnase i. this model was tested because it explains why 10-base (actually 10.4 base) multiple-related fragments are paradoxically generated by both endonucleolytic (dnase i) and exonucleolytic (exonuclease iii) mechanisms. this new model also explains the phenomenon of substantial single-stranded dna production during dnase i digesti ... | 1980 | 6249343 |
| [effect of thyrotropic hormone on the membrane potential of thyroid gland cells and thyroid hormone secretion with aging]. | effect of thyrotropic hormone (tth) on membrane potential (mp) of thyroid cells and thyroid hormone secretion was studied in experiments on male rats of two age groups (7--12- and 27--32-month-old animals). it was found that during the first 3 hours after tth administration (5 u/100 g i. v.) the depolarization of secretory cell membranes of adult rats was done pronounced and developed more rapidly than in old ones and that an increase in free thyroxin (t4) correlation with mp changes with time. ... | 1980 | 6249420 |
| a pyrimidine dimer-dna glycosylase activity associated with the v gene product of bacterophage t4. | mutations in the v gene of bacteriophage t4 are associated with a marked increase in sensitivity to killing by uv radiation at 254 nm, but not to a variety of other forms of base damage to dna. early studies from this laboratory provided evidence for a role of the v gene in the excision of pyrimidine dimers (pd) from dna. specifically, it was shown that extracts of t4v+-infected escherichia coli catalyse the formation of single-strand breaks (nicks) and/or alkali-labile sites in uiv-irradiated d ... | 1980 | 6250044 |
| uremia decreases nuclear 3,5,3'-triiodothyronine receptors in rats. | the maximal binding capacity (mbc) of hepatic t3 nuclear receptors was decreased in uremic rats (132 +/- 37 fmol/mg dna) compared to sham-operated controls (212 +/- 44 fmol/mg dna; p < 0.025), while the equilibrium affinity constants (ka) remained unaltered (1.8 +/- 0.4 and 1.5 +/- 0.3 x 10(9) m-1 in the uremic and control rats, respectively, p = ns). there was also a reduction in the mbc of the kidney t3 receptors, from 73 +/- 14 fmol/mg dna in the control animals to 32 +/- 7 fmol/mg dna in the ... | 1980 | 6250792 |
| the cellular basis for viral-induced immunodeficiency: analysis by monoclonal antibodies. | viral infections are often associated with immunodeficiency states. although t lymphocytes have been thought to suppress the host's response, the precise etiology remains unclear. therefore, we characterized t lymphocytes from six patients during both acute and convalescent phases of infectious mononucleosis (im) with monoclonal antibodies (titer, 10(-5) to 10(-7) to antigens restricted to the th2- helper (t4) and th2 suppressor (t5) t cell subsets as well as to a common t cell antigen (t3) and ... | 1980 | 6251132 |
| control of early gene expression of bacteriophage t4: involvement of the host rho factor and the mot gene of the bacteriophage. | many early mrna species of bacteriophage t4 are not synthesized after infection of escherichia coli in the presence of chloramphenicol. this has been interpreted as a need for t4 protein(s) to be synthesized to allow expression of some early genes, e.g., those for deoxycytidinetriphosphatase, deoxynucleosidemonophosphate kinase and udp-glucose-dna beta-glucosyltransferase. in the experiments described here, early mrna of bacteriophage t4 was allowed to accumulate during chloramphenicol treatment ... | 1980 | 6251243 |
| studies on phage internal proteins. vi. interaction of bacteriophage t4 internal proteins with t4 dna in vivo and in vitro. | internal proteins are basic proteins bound to the dna of t4 coliphage. centrifugation in sucrose gradients was employed to isolate dna-internal proteins complexes formed in vitro. polyacrylamide gel electrophoresis of radioactive internal proteins in the presence of sodium dodecyl sulfate followed by autoradiography of the gels was used to identify the dna-bound proteins and to determine their respective abundance in these complexes as well as in the bacteriophage head. the internal proteins wer ... | 1980 | 6251515 |
| [the thyroxine hyperthyroidism (author's transl)]. | 24 of all patients observed between 1974 and 1976 suffering from thyrotoxicosis were found to have increased blood thyroxine (t4) levels without increased triiodothyronine ones. t4 hyperthyroidisms represented 3.5% of all hyperthyroidisms observed; they were in relation with grave's disease (18 patients) or toxic nodular (6 patients); clinical pictures were quite usual; old age, severe illnesses, amiodarone and corticoids treatments, iodine excess, were sometimes being incriminated, but often no ... | 1980 | 6251556 |
| thyroid function in gestational trophoblastic neoplasia: evidence that the thyrotropic activity of chorionic gonadotropin mediates the thyrotoxicosis of choriocarcinoma. | an investigation was made of thyroid function in 20 patients with gestational trophoblastic neoplasia. two patients were judged to be overtly thyrotoxic on the basis of the symptoms and physical findings; both patients had widely metastatic choriocarcinoma, markedly increased serum t4 levels (21.4 and 27.7 micrograms/100 ml), and extremely high levels of serum human chorionic gonadotropin (hcg) (3,220 and 6,720 iu/ml) relative to those of normal gestation(< 100 iu/ml). three other patients had m ... | 1980 | 6251724 |
| a comparison of treatment modalities in carcinoma of the maxillary antrum. | sixty-two patients with carcinoma of the maxillary antrum are observed to define symptoms, physical findings, and radiologic findings suggestive of a poor prognosis. using these poor prognostic indicators, the cases are retrospectively analyzed according to the various treatment modalities that were used. it is concluded from the accumulated data that raesection of the orbital contents can be avoided in selected patients with t3 and t4 carcinoma of the paranasal sinuses. | 1980 | 6251794 |
| dependence of thyroxine utilization rate on dietary composition. | 1. the rate of utilization (k) of labelled thyroxine increased when energy intake, given as standard pig meal, was doubled from 20 to 40 g/kg body-weight per d. when the bulk of food was increased, but not its energy content, the value of k did not change. ambient temperature was constant throughout the experiment. 2. groups of pigs were given pig meal at 20 g/kg body-weight, 40 g/kg body-weight or 20 g/kg body-weight plus a supplement. the supplement was of equivalent energy content to 20 g pig ... | 1980 | 6251861 |
| den v gene of bacteriophage t4 determines a dna glycosylase specific for pyrimidine dimers in dna. | endonuclease v of bacteriophage t4 has been described as an enzyme, coded for by the denv gene, that incises uv-irradiated dna. it has recently been proposed that incision of irradiated dna by this enzyme and the analogous "correndonucleases" i and ii of micrococcus luteus requires the sequential action of a pyrimidine dimer-specific dna glycosylase and an apyrimidinic/apurinic endonuclease. in support of this two-step mechanism, we found that our preparations of t4 endonuclease v contained a dn ... | 1980 | 6252338 |
| effect of cortisol, t3 and t4, on the glucocorticoid receptor concentration in leukocytes. | 1980 | 6252389 | |
| isolation of dna from agarose gels using deae-paper. application to restriction site mapping of adenovirus type 16 dna. | a new method for isolating dna from agarose gels is described. the method involves the simultaneous transfer of all dna-fragments from an agarose slab gel onto deae-cellulose paper and the elution of the individual fragments from the paper with 1 m nacl. dna isolated from agarose gels in this way is susceptible to cleavage with several restriction endonucleases, and can be labeled in vitro with e coli dna-polymerase i, t4 dna-polymerase and t4 polynucleotide kinase. we have used the method to co ... | 1980 | 6252542 |
| [is thyroid extract necessary in the treatment of obesity? (author's transl)]. | in 273 patients divided in 12 classes according to the weight, plasma t3, t4 and tsh, thyroid response to tsh and pituitary response to trf are the same in each category. there is no reason of value for giving thyroid extract in the treatment of obesity. | 1980 | 6252608 |
| [analysis of loco-regional control rate, metastatic remission and survival in 178 breast cancer patients treated by radical modified mastectomy and post-operative irradiation (author's transl)]. | 178 cases of post-operative irradiation for breast cancer (145 t1, t2, t3 and 33 t4) are analyzed. according to the surgical procedure there are 146 cases of radical modified mastectomy (patey) and 32 cases of radical mastectomy (halsted). the axillary status reveals 50% of involved nodes, but radiotherapy has been systematic on chest wall and supra-clavicular, parasternal, axillary lymph nodes areas. loco-regional failures are only situated on chest wall (3,9%) and in the supraclavicular area ( ... | 1980 | 6252610 |
| in vivo and in vitro recombination of lambda dna in cac12 transfection. | using cacl2 mediated transfection with lambda dna fragments, in vitro joining by ligase and in vivo recombination with helper phage dna are effective systems for generating artificial recombinants. recombination efficiencies are 20--30% in the in vitro and in vivo recombination systems. at 30 to 37 degree c t4 ligase mainly joins natural cohesive alpha ends, while at 12 degrees c the ecori-generated termini are preferentially ligated to form biologically active molecules, if the cloning vector a ... | 1980 | 6252707 |
| [localization of r. smai restriction endonuclease recognition sites on the genetic map of t4 bacteriophage]. | 1980 | 6253255 | |
| glucose dependence of thyrotropin-stimulated thyroid hormone formation. | tsh (100 mu/ml) stimulation of organic binding of iodide, including formation of t4, in bovine thyroid slices was 3-fold greater when the incubation medium contained glucose; this effect of glucose was apparent at 0.1 mm and was maximal at 1 mm. tsh at 5 mu/ml enhanced organification of iodide in calf thyroid slices only with the presence of glucose in the medium. these effects of glucose are not mediated by mechanisms involving iodide transport. tsh stimulation of o2 consumption of slices was a ... | 1980 | 6253279 |
| chronic regulation of rat thyroid prostaglandin synthetase activity by endogenous thyrotropin. | prostaglandin (pg) synthetase activity was measured in the rat thyroid after hypophysectomy, t4, and methylthiouracil treatment in order to explore the possibility that pg synthetase is subject to chronic control by endogenous tsh. t4 treatment (5 mg/liter drinking water) lowered pg synthetase activity, as measured in whole homogenates, by 22% and 31% after 2 and 4 days, respectively. similarly, pg synthetase activity was reduced by 16% and 42% 2 and 4 days after hypophysectomy. methylthiouracil ... | 1980 | 6253286 |
| thyroid hormone metabolism in patients with liver cirrhosis, as judged by urinary excretion of triiodothyronine. | this study included 35 patients with liver cirrhosis, 23 patients with hyperthyroidism, 12 with hypothyroidism, and 2 with other endocrine disorders. in the various endocrine disorders an appreciable amount of triiodothyronine (t3) was excreted into the urine but the daily excretion was fairly constant in each patient. urinary excretion of t3 was negligible or depressed in hypothyroidism, but increased with a rise in the serum level of t3. serum and urinary t3 decreased in liver cirrhosis, but t ... | 1980 | 6253545 |
| dna n-glycosylases and uv repair. | repair of some dna photoproducts can be mediated by glycosylic bond hydrolysis. thus, escherichia coli endonuclease iii releases 5,6-hydrated thymines as free bases, while t4 uv endonuclease releases one of two glycosylic bonds holding pyrimidine dimers in dna. in contrast, uninfected e. coli apparently does not excise pyrimidine dimers via a dna glycosylase. | 1980 | 6253795 |
| an escherichia coli endonuclease responsible for primary cleavage of in vitro transcripts of bacteriophage t4 trna gene cluster. | an endonuclease activity was isolated from 100,000 g supernatant fraction of escherichia coli using in vitro primary transcripts of t4 trna gene cluster as assay substrates. the endonuclease cleaves the polycistronic rna precursors into fragments containing monomeric and dimeric stable rna sequences. the result strongly suggest that this enzyme participates in the early steps of t4 trna maturation pathway preceding the action of rnase p. | 1980 | 6253921 |
| apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in dna. | the characteristics of the nicks (single-strand breaks) introduced into damaged dna by escherichia coli endonucleases iii, iv, and vi and by phage t4 uv endonuclease have been investigated with e. coli dna polymerase i (dna nucleotidyltransferase). nicks introduced into depurinated dna by endonuclease iv or vi provide good primer termini for the polymerase, whereas nicks introduced into depurinated dna by endonuclease iii or into irradiated dna by t4 uv endonuclease do not. this result suggests ... | 1980 | 6254032 |
| amino acid sequence of the t4 dna helix-destabilizing protein. | the primary structure of the t4 single-stranded dna-binding protein coded by gene 32 has been determined by manual and autoated sequencing of peptides derived from partial proteolysis, cyanogen bromide cleavage, and digestion with trypsin, chymotrypsin, and staphylococcal protease. tryptic digestion of citraconylated or succinylated gene 32 protein yields five peptides containing 4, 27, 42, 65, and 163 residues, which can be separated by sephadex chromatography. each of the tryptic peptides was ... | 1980 | 6254033 |
| expression of cloned hepatitis b virus dna in human cell cultures. | dna was isolated from the ayw subtype of hepatitis b virus (hbv) that had been incubated in vitro with all four deoxynucleoside triphosphates in order to complete the circular viral genome by means of the endogenous dna polymerase. the purified viral dna was cleaved with ecori restriction endonuclease, inserted into the ecori site of plasmid pbr322, and cloned in escherichia coli chi 1776. dna from a clone, phbv-1, that contained a 3200-base-pair insert of hbv dna was cleaved with ecori and incu ... | 1980 | 6254085 |
| [behavior of thyroid hormones, corticosterone, adrenocorticotropic hormone and insulin in the plasma of the rat under stress conditions]. | in male wistar rats the behaviour of the plasma concentrations of thyroid hormones, corticosterone, adrenocorticotrophic hormone and insulin was examined in the course of a hypokinase stress of 6 weeks duration. blood was taken after a stress of 1, 3 and 6 weeks. in these cases in thyroid hormones a decrease of the thyroxine (t4) and an increase of the triiodothyronine (t3) appeared. in an increased need of energy of the organism apparently a more rapid peripheral conversion of t4 to the more ac ... | 1980 | 6254283 |
| proteolytic removal of the cooh terminus of the t4 gene 32 helix-destabilizing protein alters the t4 in vitro replication complex. | the proteolytic removal of about 60 amino acids from the cooh terminus of the bacteriophage t4 helix-destabilizing protein (gene 32 protein) produces 32*i, a 27,000-dalton fragment which still binds tightly and cooperatively to single-stranded dna. the substitution of 32*i protein for intact 32 protein in the seven-protein t4 replication complex results in dramatic changes in some of the reactions catalyzed by this in vitro dna replication system, while leaving others largely unperturbed. 1. lik ... | 1980 | 6254971 |
| comparison of the cleavage of pyrimidine dimers by the bacteriophage t4 and micrococcus luteus uv-specific endonucleases. | a comparison was made of the activity of the uv-specific endonucleases of bacteriophage t4 (t4 endonuclease v) and of micrococcus luteus on ultravilet light-irradiated dna substrates of defined sequence. the two enzymes cleave dna at the site of pyrimidine dimers with the same frequency. the products of the cleavage reaction are the same, suggesting that the scission of dna by t4 endonuclease v occurs via the combined actin of a pyrimidine dimer specific dna glycosylase and an apyrimidinic-apuri ... | 1980 | 6254991 |
| dna sequence of a t4 transfer rna gene cluster. | 1980 | 6255163 | |
| bacteriophage t4-related macromolecular synthesis under restriction of plasmid rts1. | rts1 is a plasmid which confers upon the host bacteria the capacity to restrict t4 bacteriophage growth at 32 degrees c but not at 42 degrees c. pulse-labeling of phage-infected cells showed that rts1 restricts the synthesis of t1 dna. despite efficient restriction of t4 phage growth and dna synthesis, infected escherichia coli 20so harboring rts1 synthesized both early and late t4 phage rna. synthesis of early t4 phage rna under restrictive conditions (32 degrees c) was almost equal to that fou ... | 1980 | 6255193 |
| correlation between genetic map and map of cleavage sites for sequence-specific endonucleases sali, kpni, bgli, and bamhi in bacteriophage t4 cytosine-containing dna. | cleavage sites for sali, kpni, bgli, and bamhi in cytosine-containing dna from t4 alc10(alc) nd28(dena) d2a2(denb) ame51x5(56) amn55x5(42) have been mapped relative to each other, and the positions of deletions sa delta 9 (d1-stp), r1589(rii), del(39-56)12, and tk2(ri-tk) relative to these cleavage sites have been determined. based on these analyses, a physical map of the t4 genome containing 166 kilobase pairs has been constructed. | 1980 | 6255198 |
| processive action of t4 endonuclease v on ultraviolet-irradiated dna. | the action of the dimer-specific endonuclease v of bacteriophage t4 was studied on uv-irradiated, covalently-closed circular dna. form i cole1 dna preparations containing average dimer frequencies ranging from 2.5 to 35 pyrimidine dimers per molecule were treated with t4 endonuclease v and analysed by agarose gel electrophoresis. at all dimer frequencies examined, the production of form iii dna was linear with time and the double-strand scissions were made randomly on the cole1 dna genome. since ... | 1980 | 6255442 |
| long lasting amplification and deformation of thyroid receptors after thyrotropin (tsh) and gonadotropin (gth) treatment of chickens in the foetal period. | gonadotropin or tsh treatment of the chick embryo influences the t4 production of the one-month-old animal if tsh is given on a second occasion. hormone treatment on the 8th day of life was ineffective, while previous treatment of the 12-day-old embryo resulted in a significantly more pronounced elevation of serum t4 level compared to the control when provoked by tsh in the one-month-old animal and a significant reduction in the animals subjected to gonadotropin pretreatment. | 1980 | 6266202 |