Publications

differential amplification of specific areas of phage t4 genome as revealed by hybridization to cloned genetic segments.	under various conditions, specific genetic areas of the phage t4 dna molecule are preferentially and repeatedly replicated, resulting in the amplification of these areas. these areas are found to lie in the vicinity of the known origins of dna replication.	1980	7001470
enzyme immunoassay of thyroxine in serum and dried blood samples on filter paper.	a double antibody heterologous enzyme immunoassay was developed for measuring thyroxine (t4) in serum and in dried blood samples on filter paper for use in screening for neonatal hypothyroidism. in this method, ethanol extracts of 100 microliter of serum or dried blood spots of 9 mm in diameter were incubated with t4-alkaline phosphatase conjugate prepared with glutaraldehyde, and antiserum to t4-bovine serum albumin conjugate prepared with carbodiimide. the enzyme activity in the precipitates f ...	1980	7002535
[new class of bacterial translation mutants].	two suppressor mutations of phage t4, which specifically suppress different opal mutations in the same phage t4 genome, have been obtained. one of these mutations possessed suppressor activity at 27 degrees c but not at 42 degrees c. it is shown that these suppressor mutations operate on the level of translation and probably localize in one of the genes determining the synthesis of phage t4 trna. two strains of escherichia coli b which were able to restrict this opal suppressor and did not affec ...	1980	7002726
construction of an escherichia coli strain unable to synthesize putrescine, spermidine, or cadaverine: characterization of two genes controlling lysine decarboxylase.	we have previously described a polyamine-deficient strain of escherichia coli that contained deletions in spea (arginine decarboxylase), speb (agmatine ureohydrolase), spec (ornithine decarboxylase), and sped (adenosylmethionine decarboxylase). although this strain completely lacked putrescine and spermidine, it was still able to grow at a slow rate indefinitely on amine-deficient media. however, these cells contained some cadaverine (1,5-diaminopentane). to rule out the possibility that the pre ...	1980	7002915
molecular basis for substitution mutations. effect of primer terminal and template residues on nucleotide selection by phage t4 dna polymerase in vitro.	the dna-dependent conversion of incorrect deoxynucleoside triphosphate precursors to monophosphates (turnover) by bacteriophage t4 dna polymerase was determined using either poly(da) x (dt) or poly(dg) x (dc) homopolymer templates. competition between correct and incorrect triphosphates for incorporation into dna, and the use of chain-terminating dideoxynucleoside triphosphates enabled us to determine the amount of turnover occurring at the end of each strand of the homopolymer duplex (e.g. amou ...	1980	7002928
effects of fever and hyperthermia on thyroid function.	thyroid hormones in man are affected by acute and chronic febrile states. to define these acute changes, we used a previously described rabbit model. serum levels of t3, rt3, and t4 were measured at 0, 2, 4, 6, and 24 hr following injection of 1 microgram e. coli endotoxin, and during heat-induced hyperthermia. all rabbits receiving endotoxin developed fever with peaks at one hour (delta t = 1.1 degrees c) and three hours (delta t = 1.4 degrees c); they then defervesced to base levels at 6 hr. s ...	1980	7003073
probable localization of the bacteriophage t4 prehead proteinase zymogen in the center of the prehead core.	during the assembly of the bacteriophage t4 prehead, a t4-coded protease zymogen (p21) is built into the structure. at a certain stage in head formation, the protease precursor is activated and specifically cleaves most of the prehead proteins. in this paper we show that a correlation existed between the presence of proteinaceous material in the center of the prehead core, observed by electron microscopy, and the availability of p21 during prehead assembly. in the absence of p21, the core enclos ...	1980	7003167
effects of growth conditions and mutations in rna polymerase on translational activity in vitro in escherichia coli.	the translational capacity in vitro in escherichia coli, using rna from phage r17 or q beta as messenger, is several times higher if the extracts are prepared from cells harvested in early exponential phase or grown under conditions of good aeration compared to if extracts are prepared from cells harvested in a later growth phase or grown under semi-aerobic conditions. in low activity extracts the production of phage replicase protein is preferentially affected. growth of a wild type strain unde ...	1980	7003312
antibody-producing cells in bovine lacteal secretions after local immunization.	the indirect jerne plaque assay was used to determine the presence of antibody-forming cells in lacteal secretions of 2 cows inoculated in the mammary gland with t4 phage. two adjacent mammary glands of 2 nonlactating 7-months pregnant cows were inoculated by intramammary injection 4 times at 3- to 5-day intervals. the presence of plaque-forming cells (pfc) was assessed in each quarter beginning on postinoculation day 8 and at 4- to 14-day intervals thereafter. amounts of antibodies in serum and ...	1980	7004281
[formal genetic study of the enzymology of t4 phage recombination. i. effect of the antimutational t4 dna-polymerase on noncorrection-type recombination].	effect of temperature sensitive antimutator l42 allele of gene 43 of t4 phage on the parameters of non-correction-type mechanism of genetic recombination have been studied under permissive conditions. by comparison of a point mutant with a deletion one, the critical frequency r (xi) corresponds to recombination between two markers separated by a distance equal to mean genetic length of the hybrid region was measured to be 4.15-10(-2). this value exceeds the frequency r (xi) determined with the p ...	1980	7005022
[simple method of determining the r(xi) parameter of a half-chromatid chiasma].	comparison of a point mutant of t4 phage with a deletion one in two-factor crosses against other point mutants demonstrated that at equal distances the deletion marker gave constantly lower frequencies of wild-type recombinants than the point marker did. the pattern of the distance dependence of this difference in the frequencies of recombinants agrees well with the hypothesis that mismatched regions formed by deletion markers undergo repair to the mutant allele with a probability close to unity ...	1980	7005023
modified subtotal thyroidectomy in the management of graves' disease.	a prospective study of a new surgical approach (modified subtotal thyroidectomy) in the management of graves' disease was carried out in 55 patients. the patients were followed over an average of 4 years with a strict protocol involving serial clinical and chemical evaluations (t4, t3ria, tsh) and yearly thyroid scans. long-term euthyroidism was achieved in 94% of the patients. these results are markedly superior to the functional results occurring after the standard thyroidectomy in widespread ...	1980	6892856
[clinical evaluation of the thyroid gland function based on t3, t4 and tsh activity and histopathological changes in various types of goiter].		1980	6893079
[basic and clinical evaluation of the measurement of serum thyroxine with phadebes t4 radioimmunoassay kit (author's transl)].		1980	6893173
[primary hypothyroidism as a possible cause of hypertension from long-term follow-up studies of patients with graves' disease (author's transl)].	in order to study the prognosis of graves' disease, 236 patients, who had been diagnosed as having had graves' disease more than 10 years before, were examined. although one patient had died of leukemia and 2 patients had been operated on for breast cancer after 131i therapy, and another 6 patients had died between the ages of 20 and 50, the patients were doing quite well. generally, the prognosis of graves' disease is not considered to be serious if the thyroid function is controlled. among the ...	1980	6893308
postpartum lymphocytic thyroiditis in american women: a spectrum of thyroid dysfunction.	six episodes of primary hypothyroidism followed pregnancy in five women. within 6 months post partum, all were clinically hypothyroid, with a diffuse goiter of two or three times normal size, altered thyroid function tests (average thyroxine [t4] = 2.6 micrograms/dl, thyroid-stimulating hormone [tsh] = 58.2 microunits/ml), and elevated antithyroid antibodies. (all had antimicrosomal antibody titers of 1:6,400 or more.) after 5 more months, all patients had a decrease in antibody titer, resolutio ...	1980	6893523
clinical and pathological significance of sibling graves' disease.	the clinical picture and serum antithyroid antibodies in 16 pairs of siblings with graves' disease were compared with an age and sex matched group of 32 patients with graves' disease who did not have a family history of any thyroid disease (control patients). there was a significant difference in frequency and mean titres of antibodies to thyroglobulin between sibling patients. (positive 76.0%) and control patients (positive 40.0 %), but not in microsomal antibodies (sibling; positive 92.0%, con ...	1980	6893640
thyroid hormone autoantibodies in a case of hashimoto's disease.	a case of hashimoto's disease is reported. the serum contained antibodies binding t3 and t4. the binding capacity and affinity constants for t3 were 0.03 mumol/l and 3.4 x 10(9) l/mol and for t4 0.3 mumol/l and 1.4 x 10(9) l/mol, respectively. after total thyroid lobectomy on the left and subtotal on the right the autoantibodies disappeared from the serum. thyroid tissue from the patient proved to contain an antithyroglobulin. according to the findings, the t3- and t4-binding antibodies are dire ...	1980	6893758
[a study of thyroid reserve in hashimoto's disease (author's disease)].	a three-day thyroid stimulating hormone (tsh) test of administering 10 u.s.p. bovine tsh intramuscularly was performed on 6 normal subjects, 14 patients with hashimoto's disease and 6 patients with primary hypothyroidism. the response of 24hr thyroidal 131i-uptake (131-uptake) and of serum thyroxine (t4) and triiodothyronine (t3) was studied in these subjects. of the 14 patients with hashimoto's disease, t4, t3 and tsh were normal in 5 patients (group 1); both t4 and t3 were normal and tsh was h ...	1980	6893970
in vivo and in vitro effects of adrenaline on conversion of thyroxine to triiodothyronine and to reverse-triiodothyronine in dog liver and heart.	infusion of adrenaline in healthy dogs in a dose simulating spontaneous release of the catecholamine during experimental myocardial infarction produced a significant decrease in the conversion of thyroxine (t4) to triiodothyronine (t3) and a moderate increase in the conversion of t4 to reverse-triiodothyronine (rt3). similar changes in deiodination of t4 to t3 and to rt3 were also observed when adrenaline was added in vitro to liver and heart homogenates. these results are consistent with a dire ...	1980	6783414
a site-specific rece4-independent intramolecular recombination between bacillus subtilis and staphylococcus aureus dnas in hybrid plasmids.	a composite plasmid pls253 was constructed from pls103 [carrying the bacillus subtilis leucine genes on b. subtilis (natto) plasmid pls28] and phv14 [a recombinant plasmid composed of pbr322 and the staphylococcal r-plasmid pc194] employing bamhi endonuclease, t4 dna ligase, and b. subtilis transformation. all the leu+ cmr transformants tested harbored not only pls253 but also two smaller plasmids designated as pls251 and pls252. pls253 dna, when purified on an agarose gel, retained both leu+ an ...	1980	6783475
amphetamine-induced hyperthyroxinemia.	four patients had high serum thyroxine (t4) concentrations during periods of heavy amphetamine abuse. after amphetamine was withdrawn, serum t4 returned to normal. administration of amphetamine to monkeys induced a rise in serum t4; in this model the high t4 level appeared to be caused by increased serum thyrotropin. the mechanism of this effect is unclear but is presumably mediated via the hypothalamus. awareness of transient hyperthyroxinemia due to amphetamine may allow the physician to avoid ...	1980	6782925
bromocriptine suppresses the thyrotrophin response to thyrotrophin releasing hormone during human pregnancy.	thyrotrophin (tsh) responses to 200 microgram of intravenous thyrotrophin releasing hormone (trh) were measured in fifteen healthy women in normal early pregnancy before and at the end of a bromocriptine treatment of 5.0-7.5 mg daily for 1-2 weeks. bromocriptine did not change the basal levels of tsh, triiodothyronine (t3) and thyroxine (t4) during pregnancy. before the start of bromocriptine, trh caused a significant tsh elevation from 12.8 +/- 0.5 muu/ml (mean +/- se) to 21.2 +/- 1.9 muu/ml af ...	1980	6783349
thyroid function in multiple sclerosis.	thyroid function was studied in 112 ms patients and 175 controls on the basis of t3, t4, t.b.g. and t.s.h. radioimmunoassays. in 33 ms patients with t.r.h. stimulation test was employed additionally. the ms patients had significantly increased t4 levels whereas t3 and t.s.h. were found to be significantly lower. no differences were found in the t.r.h.- stimulated t.s.h. response. the changes in thyroid function parameters were similar in different courses of ms and did not correlate with the deg ...	1980	6782819
hormone profile of t3, t4 at the end of pregnancy and during puerperium.	the authors have studied the t3, t4 and tbg behaviour at the end of pregnancy and after 96 h and 144 h from delivery in 25 pregnant women. the patients have been randomly divided into two groups: the first was studied in basal conditions and the second after administration of enantate testosterone and valerianate estradiol. in the first group t3 and t4 values increased slightly. a higher increase was noticed from the 96th to the 144th h from delivery. in the second group t3 and t4 values were di ...	1980	6781794
hyperthyroidism and pregnancy. i. serum tbg level in normal pregnancy.	methods for preparation and qualitative evaluation of the reagents necessary for direct radioimmunoassay of thyroxin-binding globulin (tbg) in serum are described. the reagents are: purified tbg necessary as antigen in preparing the labelled product and plotting the reference curve; 125i-tbg; anti-tbg rabbit serum. the technical stages characteristic of the ria system are presented. tbg level determined in normal volunteers (about 80% males and 20% females, young subjects living in bucharest are ...	1980	6782647
surface charge and hydrophobicity of salmonella, e. coli, gonococci in relation to their tendency to associate with animal cells.	the surface charge and hydrophobicity of salmonella typhimurium, escherichia coli and neisseria gonorrhoeae bacteria have been compared with their tendency to associate in vitro with human polymorphonuclear leucocytes (pmnl) and hela cells in culture. it was found that lipopolysaccharide mutations in s. typhimurium or e. coli, yielding core-defective mutants, increased the surface hydrophobicity, as assessed by aqueous two-phase partitioning and hydrophobic interaction chromatography, and promot ...	1980	6782659
[thyroid hormone levels after surgical stress reaction (author's transl)].	in a larger collective of surgical patients the behaviour of the individual thyroid parameters (t4, t3, rt3, tbg and thyroxine-binding capacity) were studied before, during and after operation and set in relation to each other. there are significant shifts in t4 metabolism towards the formation of rt3. in spite of its greatly reduced metabolic activity compared with t3, an important regulatory effect is attributed to rt3, in thyroid metabolism in stress situations.	1980	6780844
[prolactinomas and "idiopathic" hyperprolactinemia. case series].	endocrine features in 22 women affected by idhyopatic hyperprolactinemia and in ten women affected by prolactin secreting adenoma have been assessed basic levels of fsh, lh, e2, t3, t4, and prl were evaluated and dynamic tests by gnrh, trh and bromocryptine were performed prolactin basal value and trh dynamic test show some value in differentiating hydiopatic hyperprolactinaemia from prolactynomas, but no hormon by itself nor dynamic test show a reliable effectiveness for this diagnosis.	1980	6781058
linear modulation of serum thyrotrophin by thyroid hormone treatment in hypothyroidism.	basal serum tsh and the peak tsh response to a 500 micrograms trh bolus were measured in 57 euthyroid and in 29 hypothyroid subjects either receiving graded thyroid hormone replacement or acutely removed from full replacement therapy. serum tsh, total t4 and t3 were determined by sensitive radioimmunoassay methods. the peak versus basal tsh data for hypothyroid patients were linear within individuals. the regression slope of the peak versus basal tsh data for all hypothyroid subjects did not dif ...	1980	6779474
alteration of thyroid function in the early stage of subacute thyroiditis.	measurement of serum triiodothyronine (t3), thyroxine (t4), free triiodothyronine (ft3), free thyroxine (ft4), thyroxine-binding-globulin (tgb), antithyroglobulin antibodies (anti-htg), thyroid 131i uptake and scanning was performed on 12 patients during the early phase of subacute thyroiditis. serum thyrotropin (tsh) was measured during baseline conditions and following administration of synthetic thyrotropin-releasing-hormone (trh). the stimulation with exogenous tsh was performed on 7 subject ...	1980	6779475
[plasma tbg, t3 and t4 levels in the pregnant and non-pregnant women (author's transl)].		1980	6780251
influence of exposure to moderate altitude on the plasma concentraton of cortisol, aldosterone, renin, testosterone, and gonadotropins.	the influence of 11 days at moderate altitude (2,000 m) combined with exercise on plasma concentration of testosterone, fsh (follicle-stimulating hormone), lh (luteinizing hormone), cortisol, aldosterone, and renin activity was studied in ten healthy subjects. within 48 h of arrival at moderate altitude a significant increase in testosterone was found whereas fsh had decreased significantly and lh showed a tendency to decrease. cortisol increased significantly at the beginning and reached a maxi ...	1980	6780338
serotonergic and catecholaminergic influence on thyroid function in the vervet monkey.	vervet monkeys were pharmacologically treated acutely and with repeated dose loading to alter serotonergic systems to assess the role of serotonin in the regulation of the hypothalamus-pituitary-thyroid axis. acute l-tryptophan administration failed to alter basal levels of thyroid hormones but did decrease the trh-induced tsh response. repeated dose loading of tryptophan or 5-hydroxytryptophan increased blood serotonin and plasma t3 and decreased plasma tsh. the tryptophan hydroxylase inhibitor ...	1980	6780370
kinetics of the cooperative association of t4 tail sheath protein p18 to polysheaths.	the polymerization of the monomeric sheath protein p18 to polysheath was followed by light scattering in 1 mm sodium phosphate buffer, ph 7 at a mgcl2 concentration of 5 mm. sigmoidal kinetics were observed in the case of spontaneous nucleation. these were well fitted by a mechanism involving a slow nucleation step (rate constant kn = 10(-2) m-1 s-1) followed by propagation steps (k = 10(5) m-1 s-1) in which p18 protomers are added to the ends of the polysheath particles. when sonicated polyshea ...	1980	6452915
[improved thyroid diagnosis in borderline hyperthyroidism by determination of free t3 index].	the measurements of circulating total thyroxine (t4) and total triiodothyronine (t3) depend on the concentrations of thyroid hormone binding proteins, especially tbg. a simple approach, the calculation of free t3 index (ft3i) and augmented ft3i (aft3i) from total t3 and t3-resin uptake, corrects the total serum t3 for variation in thyroid hormone binding capacity. in a group of 95 patients with borderline elevated t3 levels (3.0-4.5 nmol/l) we have correlated the results of ft3i and aft3i with c ...	1980	6792700
[reciprocal changes in t3 nd rt3 serum concentrations in digestive diseases resulting in childhood protein-calorie malnutrition (author's transl)].	in 14 children affected by digestive diseases producing protein-calorie malnutrition t3 and rt3, t4, tsh serum levels and tbg and tbpa maximum binding capacity were measured at the moment of the hospitalisation and up to their clinical and biochemical amelioration. no changes in serum t4 and tsh levels and in tbgcap values were detected. tbpacap was found to be persistently low. at the moment of the hospitalisation serum t3 and rt3 average values were found to be 65 +/- 25 ng/dl and 58 +/- 25 ng ...	1980	6786206
tsh, hgh, hpr, and cortisol response to trh in depressed patients.	an intravenous (0.2 mg) and an oral (40 mg) trh-test was performed on 21 depressed patients (4 psychogenous, 17 endogenous); tsh, hgh, hrr, cortisol, t3, t4 and rt4 were measured. the test was redone after 3 weeks of antidepressive treatment. using an oral stimulation, it looks like there would be a possibility to differentiate into different groups according to the severity of illness. it also seems that the oral stimulation is a possibility to get information on the beginning effectiveness of ...	1980	6792865
t4-induced metamorphosis in ambystoma gracile (baird) from two populations: the effects of aging and temperature.	in order to determine the factors affecting the maturation of the functional hypothalamo-pituitary-thyroid axis, ambystoma gracile (baird) larvae of different ages and acclimated to different temperature regimes were exposed to a concentration of thyroxine (t4) which activates the pituitary-thyroid axis. a. gracile from a montane and low-altitude population were utilized. these studies, plus observations concerning spontaneous metamorphosis, indicated that populations of ambystoma gracile are hi ...	1980	7348194
influence of experimental hypothyroidism on chick myogenesis.	the influence on chick myogenesis of hypothyroid status induced in ovo by methimazole was histologically (number and types of muscle fibers) and biochemically studied. a single injection of methimazole induced an hypothyroid status during embryogenesis, as shown by the plasma t4 levels which were separately assayed in male and female controls and treated embryos from day 12 of incubation to day 4 after hatching. in both sexes, control plasma t4 reached a peak on day 20 of incubation, but the fem ...	1980	7349420
effect of triiodothyronine on bronchial asthma. iii.	in two previous communications we demonstrated that triiodothyronine (t3) improved cases of chronic bronchial asthma (cba). in this communication we administered t3 in a dose of 40 microgram/day for a period of sixty days to twelve cases of cba aged less than 38 years who had had the condition for more than 5 years and were not on steroids or chromoglycate. all were outpatients. respiratory functions including vital capacity and timed expiratory volume were tested before and after the period of ...	1980	7263583
increased serum thyroid hormone binding in narcotic addicts is due to liver disease.	the cause of increased thyroid hormone binding in narcotic addicts has been investigated. the average t3, t4, and t3 binding ratio were all significantly higher in addicts than in controls. free t4 and free t3 indices were normal. those patients who had abnormally high t3 binding ratios had significantly more severe abnormalities of liver function than the other methadone-treated addicts. together with evidence that chronic active hepatitis in the nonaddicted population is associated with increa ...	1980	7258169
evaluation of l-thyroxine requirement in treatment of congenital hypothyroidism.	the optimal daily requirement of l-t4 in replacement therapy for hypothyroidism was evaluated in evaluated in 24 cases of congenital hypothyroidism and 3 of juvenile myxedema, ranging from 3 to 30 years of age. the optimal dose of l-t4, defined as the minimal dose necessary to suppress the serum concentration of tsh into the normal range, was determined in each patient. the optimal dose of l-t4 between 3 and 5 years of age was 5-7 microgram/kg/day, which was higher than a level of 2-4 microgram/ ...	1980	7262034
a solid-phase radioimmunoassay for free thyroxine in serum compared with equilibrium dialysis method.	serum free thyroxine (t4) was measured by a solid-phase radioimmunoassay in various thyroid states and in those associated with changes in serum t4-binding proteins. and it was compared with the value obtained by product of total serum t4 and of per cent free t4 by equilibrium dialysis. serum free t4 concentrations were found to be 1.34 +/- 0.33 ng/100 ml (mean +/- s.d.) in 51 normal subjects, 5.02 +/- 1.41 ng/100 ml in 29 hyperthyroid patients, 0.16 +/- 0.15 ng/100 ml in 12 hypothyroid patients ...	1980	7256723
measurement of triiodothyronine in urine.	a radioimmunoassay procedure to measure triiodothyronine (t3) in unextracted urine is described. one hundred microliter of t3 standards or samples were incubated with 100 microliter of t3 antiserum (1:40,000), 100 microliter of tracer 125i-t3 and 700 microliter of 0.05 m borate buffer, ph 8.6. separation of free from bound antigen was achieved by dextran coated charcoal. the cross-reaction of l-t3 with l-t4 in this assay system was less than 0.2%. dilution of high t3 urine was parallel to the st ...	1980	7256725
effects of open-heart surgery on thyroid hormone levels.	the effect of heart surgery with and without cardiopulmonary by-pass on plasma levels of thyroxine (t4) and triiodothyronine (t3) was studied in 19 patients. it was found that even before the operation the plasma levels of t4 and of t3 were significantly lower in the by-pass group of patients in comparison with those without by-pass. this lower level of thyroid hormones could be explained by greater pre-operative stress. this presumption is supported by the finding of the greater pre-operative p ...	1980	7244397
effect of thyroid hormone on the serum level of somatomedin a.	the effect of thyroid hormone on the serum level of somatomedin a was studied using a radioreceptor assay for somatomedin a. in 13 patients with hyperthyroidism, the mean level of somatomedin a was 0.06 +/- 0.05 u/ml, which was within the normal range. low levels were found in 19 patients with primary hypothyroidism with a mean of 0.39 +/- 0.04 u/ml. after treating these patients with thyroxine, the serum somatomedin a levels returned to the normal range. there was a significant correlation betw ...	1980	7238422
[behavior of tsh, t4, free t4, t3, free t3 and reverse t3 in the first 15 days of life].		1980	7254144
[basic and clinical evaluation of corning total t4 ria kit (author's transl)].		1980	7244310
differential inactivation of three bacteriophages by acid and alkaline ph used in the membrane adsorption-elution method of virus recovery.	the study was prompted by our inability to concentrate phages by a membrane adsorption method effective for polioviruses. consequently two coliphages wpk and t4, and f116 of pseudomonas aeruginosa were tested for their resistance to acid (ph 5.2-3.2) and alkaline (ph 10-11.5) exposures. only t4 proved acid resistant allowing for acid adsorption, and only wpk was sufficiently alkaline resistant to allow for alkaline elution. thus, the differential susceptibility of various phages precludes the us ...	1980	7237265
squamous carcinomas of the head and neck: some patterns of spread.	patterns of tumour spread are examined in 160 patients with squamous carcinomas of the head and neck with reference to perineural infiltration, direct invasion of bone and ossified cartilage, and lymph node metastases in the previously irradiated neck. perineural spread is comparatively common in large (t3, t4) tumours; it may be apparent early in the disease; it is often detectable clinically; and it is an adverse prognostic feature which may modify clinical management. direct bone invasion is ...	1980	7230216
determination of mutation rates in bacteriophage t4 by unneighborly base pairs: genetic analysis.		1980	7218357
inhibition of t4 bacteriophage yield by 9-anilinoacridines; comparison with in vivo antitumour activity.		1980	7227441
selective 32p-labelling of individual species in a total trna population.	a simple procedure to label individual trna species in a total trna preparation has been developed. the principle of the method is as follows: total crude trna (from e. coli) is incubated in the presence of a crude aminoacyl-trna synthetase preparation, containing most aminoacyl-trna synthetases and only one specific amino acid corresponding to the trna species which is intended to be labelled. this achieves the purpose of charging the desired trna species thereby protecting its 3'oh-terminus; o ...	1980	7008026
[endocrine changes in chronic renal failure].	endocrine abnormalities are common symptoms in patients with chronic renal failure. they are characterized by: 1. excessive somatotropin and prolactin secretion, 2. increased peripheral conversion of t4 to rt3, 3. secondary hyperparathyroidism accompanied by a relative insufficiency of calcitonin secretion, 4. hypogonadism, 5. hyperinsulinism and 6. decreased erythropoetin and 1,25-dihydroxy-vitamin d synthesis. in patients with chronic renal insufficiency normal function of the renin-angiotensi ...	1980	7008396
[therapy, follow-up treatment and prevention of urinary bladder cancer].	bladder carcinoma is a disease with a poor prognosis if the tumor is of advanced stage: the 3-year survival rate in stage t3 and t4 is 21 and 4%, respectively. superficial, noninfiltrating lesions, i.e., ta and t1 (82% 3-year survival rate), have a considerably more favorable prognosis, but on the other hand are associated with considerable morbidity. recurrent tumors can be expected in 50-90%, and more than five transurethral resections are necessary in 12.7% of the cases. treatment modalities ...	1980	7005785
purification of the t4 dna ligase by blue sepharose chromatography.		1980	7006454
iodine metabolism and iodothyronine (t3 and t4) binding in human polymorphonuclear leucocytes in vitro.	surviving human polymorphonuclear leucocytes (pmnl) were found to incorporate and metabolize 125i in vitro. after incubation the metabolized iodine may be found in three different fractions which may be separated by sephadex g-200 column chromatography. the first fraction consists of deoxyribonucleoproteins and specifically binds t3 (bmax) 4 x 10(-12) mol mg-1 protein; ka (4.5 x 10(11) mol-1 mg) and t4 (bmax) 13 x 10(-12) mol mg-1 protein; ka (0.77 x 10(11) mol-1 mg). the second fraction binds i ...	1980	6970122
bacteriophage t4 morphogenesis as a model for assembly of subcellular structure.	the sequence of steps in bacteriophage t4 assembly has been elucidated by using a combination of genetic, biochemical, and ultrastructural techniques. the phage head, tail, and tail fibers are assembled via independent pathways, and then are jointed to form the complete virus. current knowledge of these three pathways is reviewed briefly. two general insights emerging from phage assembly studies are (1) a realization of the importance of kinetic controls, and (2) recognition of the role of nonst ...	1980	7022546
dna-rna polymerase complexes associated with the membrane from bacteriophage t2- or t4-infected escherichia coli. i. general properties.	properties of dna-rna polymerase complexes, apparently bound to a fraction of the cell membrane of bacteriophage t2- or t4-infected escherichia coli, are described. evidence is presented to show that the complexes initiate the asymmetric synthesis of rna, and release the finished product. the transcription capacity per unit of beta' + beta was 10 times higher at 6 min than at 30 min after infection.	1980	7011383
dna-rna polymerase complexes associated with the membrane from bacteriophage t2- or t4-infected escherichia coli. ii. a comparison of in vitro rna with the in vivo rna products.	membrane preparations, containing dna-rna polymerase from bacteriophage t2- or t4-infected escherichia coli, caused the asymmetric synthesis of rna classes similar to those produced sequentially in vivo: 1, pre-early rna was formed when a membrane preparation was isolated 1 min after infection. 2, pre-early and delayed early rna, in roughly the same proportion as that obtained in vivo, was synthesized by a preparation obtained 6 min after infection. 3, pre-early, delayed early and late genes wer ...	1980	7011384
effects of methadone on thyroid function in mother, fetus, and newborn.	the effects of maternal use of methadone during pregnancy on tests of thyroid function were studied in 14 women and their offspring. twelve normal nonaddicted women and their infants served as a control group. concentrations of thyrotropin (tsh), thyroxine (t4), and triiodothyronine (t3) were not significantly different in maternal and cord serum, and in infant serum at age 24 hours. there was no significant difference in infants' tsh concentrations at 2, 3, and 7 days of age. at 2 and 7 days, t ...	1980	7189054
interrelationships between sex hormone-binding globulin and 17 beta-estradiol, testosterone, 5 alpha-dihydrotestosterone, thyroxine, and triiodothyronine in prepubertal and pubertal girls.	sex hormone-binding globulin (shbg), 17 beta-estradiol (e2), testosterone (t), 5 alpha-dihydrotestosterone (dht), t4, and t3 were measured in prepubertal (age, 1--8 yr; n = 44; group i) and pubertal (age, 9--16 yr; n= 56; group ii) girls. the main results were as follows. 1) in group i, a higher mean (+/- sem) value for shbg was found (73.5 +/- 2.82 nmol/1) compared to that in group ii (56.8 +/- 3.37; p less than 0.001). 2) despite a significant increase of the androgens t and dht with age in gr ...	1980	7189524
hyperthyroidism due to a pituitary tsh secreting tumour with amenorrhoea-galactorrhoea.	a 20-year-old female presented with thyrotoxicosis associated with amenorrhoea and galactorrhoea after oral contraceptive withdrawal. serum tsh was persistently elevated (mean: 28 +/- 3.1 microu/ml during 24-h sampling and did not vary significantly after trh, bromocriptine or somatostatin. prolactin levels remained constantly at the upper limit of normal (mean: 20.6 +/- 2.1 ng/ml, with a minimal increase after trh, a slight decrease after somatostatin (54%) and a marked decrease after bromocrip ...	1980	7189698
postnatal changes in concentrations of serum and urinary thyroxine and 3,3',5-triiodothyronine in the pig.	urine and serum obtained from piglets at birth (0 hr), 6, 12, 24, 48, 72 and 240 hr postpartum were assayed for thyroxine (t4) and 3, 3', 5-triiodothyronine (t3) concentrations. urine was also quantified for total iodine. piglet body weights and hematocrits were also recorded at each sampling time. serum t4 concentrations were higher (p < .05) at birth (0 hr) than at any other time. by 24 hr, serum t4 decreased (p < .05) to a concentration similar to that observed at 240 hours. in contrast, seru ...	1980	7190965
[neonatal intestinal obstruction due to congenital hypothyroidism (author's transl)].	three children with subacute neonatal intestinal obstruction are described in whom the diagnosis of congenital megacolon was suspected. the radiological changes improved when oral feeding stopped but reappeared when it was restarted. the aetiology remained unrecognised until the results of the neonatal screening for hypothyroidism showed all three cases had congenital hypothyroidism. the severity of the disorders was unusual and remains unexplained. systemic tsh and/or t4 determinations should l ...	1980	7191699
24-h profile of serum rt3 and serum 3,3'-t2 in normal man.	in a previous study we found a night surge in serum free t3 varying in parallel with that of serum tsh. in order to evaluate whether diurnal alterations in peripheral iodothyronine monodeiodination may be involved we have measured two products of peripheral deiodination, 3,3',5'-t3 (rt3) and 3,3'-t2 in serum samples obtained at short intervals during a 24-h period in 5 normal male subjects. serum rt3 was rather stable during the period albeit with a trend towards lower levels during the night wh ...	1980	7192042
relation of cord blood thyroxine and thyrotropin levels to gestational age and birth weight.	a program of screening cord blood for evidence of primary neonatal hypothyroidism was implemented in a general hospital. in 13 months 3456 newborns were screened: the thyroxine (t4) and triiodothyronine (t3) concentrations were measured in cord blood samples, and when the t4 level was below 8.0 micrograms/dl thyrotropin was also assayed in the sample. the two-tier program was effective. one hypothyroid newborn was detected and treated. more boys than girls had t4 levels below 8.0 micrograms/dl ( ...	1980	7192594
therapy of primary hypothyroidism with l-triiodothyronine: discordant cardiac and pituitary responses.	cardiac systolic time intervals were studied in ten patients with primary hypothyroidism before and during therapy with increasing doses of oral l-triiodothyronine (l-t3). therapy was increased sequentially from 10, 20, 25 to 50 microgram of l-t3 daily on a monthly basis. on l-t3, 20 to 25 microgram/day, cardiac systolic time intervals and other peripheral responses to thyroid hormone including serum cholesterol concentration, serum creatine phosphokinase (cpk) activity, and basal metabolic rate ...	1980	7226568
the effect of fenclofenac on thyroid function tests in vivo and in vitro.	clinically euthyroid patients on long term maintenance therapy with the non-steroidal anti-inflammatory drug fenclofenac (flenac) show an unusual and abnormal pattern of serum thyroid function tests. in all twelve patients studied, total t4 concentrations were grossly subnormal (mean 28.4 +/- 9.9 (sd) nmol/l and total t3 levels low-normal (mean 1.4 +/- 0.3 (sd) nmol/l), whereas rt3 (mean 0.36 +/- 0.06 (sd) nmol/l) and basal tsh levels (mean 1.9 +/- 0.5 (sd) mu/l) were within their respective nor ...	1980	7226571
inhibitor studies on phage t4 wild-type and mutant dna polymerase. iv. the substrate analog 3'-fluorothymidine 5'-triphosphate.	the deoxythymidine-5'-triphosphate (dttp) analog 3'-fluorothymidine 5'-triphosphate (3'-fdttp) inhibits dna synthesis by t4 wild-type, l98 (mutator) and cb121 (antimutator) dna polymerase. cb121 dna polymerase is less sensitive by a factor of two than the l98 and t4+ enzymes. inhibition is not due to incorporation of the analog into dna. 3'-fdttp acts competitively to the substrate dttp. the cb121 polymerase exhibits a higher ki to km ratio than the other two enzymes (5.3 vs. 3.3) and thus discr ...	1980	7222744
the effect of atenolol on serum thyroid hormones in hyperthyroid patients.	the effect of the beta-1-adrenoceptor blocking agent, atenolol, on serum levels of thyroxine (t4), triiodothyronine (t3) and reverse triiodothyronine (rt3) was studied in twelve hyperthyroid patients. while all patients improved symptomatically after treatment with atenolol 100 mg twice daily for 2 weeks, there were no significant changes in the serum t3 or rt3 concentrations. the serum t4 level showed a small but statistically significant fall. these data do not support the hypothesis that the ...	1980	7214722
[determination of thyroxine in serum by ria kit of amerlex t4 (author's transl)].		1980	7208997
inhibitor studies of phage t4 wild-type and mutant dna polymerases. iii. distamycin a, actinomycin d, adriamycin, daunomycin and ethidium.	adriamycin, daunomycin and ethidium, intercalating drugs which bind to double- as well as single-stranded dna, inhibit phage t4 antimutator mutant cb121 dna polymerase more strongly than the t4 wild-type and the mutator l98 polymerase. in contrast, all three enzymes are inhibited equally by distamycin a and actinomycin c. the latter two inhibitors bind only to double-stranded dna.	1980	7210707
[evaluation of the efficacy of cis-diamino-dichloro-platinum as monochemotherapy in the treatment of advanced malignant tumours of the bladder (author's transl)].	the authors report the results of a trial of the treatment of recurrences of advanced carcinomas of the bladder using cis-diamino-dichloro-platinum administered by continuous iv infusion over a period of 24 hours at a dose of 100 mg/m2, under cover of hyperhydration and anti-haematizing drugs. the trial involved 10 patients who has had the following initial treatment : - excision for 8 of them (2 total cystectomies for t4, one total cystectomy for t3b, 2 total cystectomies for t3a and 3 partial ...	1980	7193229
in vitro hepatic deiodination of l-thyroxine to 3,5,3'-triiodothyronine in cold-acclimated rats.	an in vitro model has been employed to evaluate the effect of cold acclimation on peripheral outer ring deiodination of thyroxine (t4) to triiodothyronine (t3) by 9,000 g supernatants of fresh liver homogenates. hepatic t3 generation from t4 in male rats exposed to 4 +/- 1 degree c for 12 wk was 41 and 24% higher than control after 30- and 60-min incubation periods, respectively. this was associated with a 49% increase in serum t3 concentration in the cold-acclimated rats, whereas serum t4 conce ...	1980	7204159
iodine treatment of iodine-induced thyrotoxicosis.	a 62-year-old female who had received prolonged iodine therapy for asthma presented with severe thyrotoxicosis and severe asthma. her history, elevated serum thyroxine and triiodothyronine, low 131i uptake, and elevated intrathyroidal iodine content by fluorescent scan were most consistent wiht a diagnosis of iodine-induced thyrotoxicosis (iitt). the clinical course of her thyrotoxicosis was protracted, and in spite of its etiologic role in the precipitaton of thyrotoxicosos, iodine was therapeu ...	1980	7204887
serum t3, t4, tsh patterns in euthyroid subjects treated with 131i for thyrotoxicosis.		1980	7205396
circulating thyroxine levels in institutionalized children.	the circulating levels of total thyroxine (t4) and normalized thyroxine (t4n) were studied in 41 institutionalized children (ic) and in 22 control children (cc), from two to six years old. no difference were observed in the values of t4 between the two groups but in ic, t4n levels are inferior to those in cc. the periods children have been confined, their chronological age and their age at the time of admittance greatly influence these results. the differences between ic and cc become important ...	1980	6167222
morphologic alterations in the trachea and the salivary gland following the induction of rapid synchronous vitamin a deficiency in rats.	the use of the synchronous induction method enables both assessment of the sequence and reliability of the appearance of morphologic signs of vitamin a deficiency, and their accurate correlation with biochemical and physiologic abnormalities. in the trachea, hyperplasia of basal epithelial cells was observed by day 4 (t4) following the withdrawal of retinoic acid from retinoate-cycled, stringently deficient rats. keratinization was observed by day 6, the upper part of the trachea showing the hig ...	1980	6153866
combined intraarterial chemotherapy, radiation therapy, and surgery for advanced squamous-cell carcinoma of the head and neck.	from 1973 to 1978, 20 patients with t3 and t4 squamous cell carcinoma of the head and neck were treated according to a triple-therapy protocol at the university of oregon health sciences center. intraarterial chemotherapy (iac) with bleomycin and methotrexate was given prior to high-dose preoperative radiation therapy and then, when possible, the lesion was resected. the treatment results are presented. the initial clinical response to iac and radiation correlated well with the findings in the p ...	1980	6154515
synthesis of 5' fragments of formylmethionine transfer ribonucleic acid and their reconstitution with a natural three-quarter molecule.	an eicosanucleotide c--g--c--g--g--g--g--u--g--g--a--g--c--a--g--c--c--u--g--gp corresponding to the bases 1--20 of the nascent sequence for the escherichia coli trnafmet has been synthesized by the joining of the chemically synthesized oligonucleotides c--g--c--g, g--g--g--u--g--g and a--g--c--a--g--c--c--u--g--gp using rna ligase from t4-infected e. coli. the hexanucleotide and decanucleotide were phosphorylated with polynucleotide kinase and [gamma-32p]atp prior to the joining reactions. the ...	1980	6154576
a dna-dependent atpase from t4-infected escherichia coli. purification and properties of a 63,000-dalton enzyme and its conversion to a 22,000-dalton form.	a dna-dependent atpase with a molecular weight of 63,000 has been purified to near homogeneity from t4-infected escherichia coli. this enzyme is not present in uninfected e. coli. it is present in e. coli infected with amber mutants of gene 44 or gene 62 and with the data mutant. the enzyme can be eluted from single-stranded dna-cellulose at nacl molarities greater than 0.6 m and from atp agarose with 80 mm mgatp. the enzyme hydrolyzes mgatp or mgdatp to the diphosphates with equal maximum veloc ...	1980	6154701
specific labeling of 3' termini of rna with t4 rna ligase.		1980	6154874
the effects of long-term antithyroid drug treatment on serum reverse t3 in patients with graves' disease.	the effects of long-term treatment with antithyroid drugs, carbimazole (cmi) or propylthiouracil (ptu), on serum reverse triiodothyronine (rt3) levels were studied in 23 patients with graves' disease. nineteen patients were given cmi and four ptu for a minimum of six months. after one month of treatment the serum levels of thyroxine (t4), triiodothyronine (t3) and rt3 had normalized in both groups. when l-thyroxine was added to the regimens after two months of therapy, both serum t4 and rt3 leve ...	1980	6155758
the effects of tetraalkylammonium salts on helix-coil transition parameters in natural and synthetic ribo- and deoxyribo-polynucleotides.	thermal transition profiles were recorded for a variety of natural and synthetic dna and double-stranded rna preparations in the presence of tetramethylammonium (tma+) and tetraethylammonium (tea+) cations. double-stranded rnas of natural origin, with gc contents of 50% exhibited the same profiles and tm values as native dna containing normal bases. hence the tetraalkylammonium cations liquidate not only the effects of base composition, and the difference in stability between a-t and a-u base pa ...	1980	6156017
effect of thyroxine and ethanol on cerebral nucleic acids during early postnatal period in rabbits.	in the view of the fact ethanol increases the blood-brain barrier permeability for thyroxine (t4) during a critical period of postnatal brain development in the rabbit, the influence of ethanol with or without t4 on cerebral rna and dna concentration has been investigated. generally accepted measures of thyroid hormone action on developing neural tissue such as the changes in the nucleic acids, cell density and size and in protein level, dry matter and cholesterol have been used. ethanol alone c ...	1980	6156818
rnase p of bacillus subtilis has a rna component.	ribonuclease p from bacillus subtilis cleaves a gln-leu trna dimeric precursor from bacteriophage t4-infected escherichia coli, yielding products identical with those generated by the e. coli rnase p. using this trna dimer as an assay substrate, the rnase of p of b. subtilis was shown to consist of at least two components, one of which bands in cscl equilibrium buoyant density centrifugation at 1.7 g/ml, characteristic of a protein x nucleic acid complex. both this component and a second, retrie ...	1980	6156938
[the lactate-pyruvate quotient in the framework of modern thyroid diagnosis].	in literature a big number of central and peripheral parameters for the diagnosis and control of therapeutical measures in diseases of human thyroid gland is described. that means, that none of the methods used has a sufficient weight. the enzymatic estimation of pyruvate and lactate reflect the peripheral effect of the hormones of thyroid gland. the pyruvate and lactate levels differ in the several groups of patients with euthyreosis, hypothyreosis, hyperthyreosis and euthyreotic goitre. betwee ...	1980	6157257
specific t helper and suppressor cells and factors induced in vitro to the hapten np.	nitrophenyl (np)-specific helper cells and suppressor cells were induced in vitro using np-t4 bacteriophage as antigen. these cells could mediate their effects also by secreted effector molecules, helper and suppressor factors. the function of both np-specific helper and suppressor cells was abolished by treatment with anti-thy1.2 plus c', but they were not retained on nylon wool columns, suggesting that np-specific helpers and suppressors were t cells. the membrane phenotype of both np-specific ...	1980	6157346
effect of clonidine on blood pressure alterations induced in spinal cats.	blood pressure alterations were evoked by stimulation of spinal pathways descending in the dorsolateral funiculus of chloralose-anesthetized cats, spinalized at the c1 level. intravenous clonidine was found to attenuate threshold, intermediate, and maximal pressor responses (elicited by increments in current delivery) in a dose-related manner. the pressor responses following norepinephrine administration (0.2 micron/kg, i.v.) were somewhat potentiated by clonidine in these animals. furthermore, ...	1980	6157959
location of t cell and major histocompatibility complex antigens in the human thymus.	a series of monoclonal antibodies were used to study the intrathymic distribution of t cell-specific antigens, ia antigens, and beta 2-microglobulin in frozen sections of human thymus by immunofluorescence and immunoperoxidase techniques. most of the cortical thymocytes reacted with anti-t4, anti-t5, anti-t6, anti-t8, and anti-t10 antibodies, thus indicating coexpression of multiple antigens on cortical lymphocytes. the staining of cells in the medulla was most satisfactorily judged in sections ...	1980	6158549
[role of the beta-subunits of escherichia coli rna-polymerase in the regulation of gene activity].	the dna-dependent rna-polymerase from e. coli b/r and its rif-r mutant rpob409 with pleiotropic effect has been studied. it was shown, that multiple forms of promotor sites in t4- and t7-dna "early" regions are recognized with different efficiences by rna-polymerases from e. coli b/r and rpob409. the rif-r rpob409 mutation has been reported to affect the beta-subunit. thus, the present data indicates that the selection of promoter sites can be controlled by the beta-subunit of rna-polymerase.	1980	6158672
[t-proteins of streptococcus pyogenes. ii. communication: preparation of specific immunoadsorbents for isolation of anti-t-antibodies and investigations of the t4/24...pattern (author's transl)].	the t4-antigen(s) in streptococcus pyogenes standard type strains of the t4/24...pattern was (were) investigated in regard to the uniformity or diversity of the antigen(s) in this t-complex. tryptic t-extracts of types 4, 24, 26, 28, 29, 46, 48, 60 were purified by ion exchange chromatography on deae-cellulose. anti-t4-antibodies were isolated by immunoadsorption chromatography on ah-sepharose linked t4-antigen. purified t4-antigen showed in sds-electrophoresis a similar multiple molecular size ...	1980	6158813
prognostic indicators in induction cis-platinum bleomycin chemotherapy for advanced head and neck cancer.	a complete response to induction cis-platinum bleomycin chemotherapy significantly increases the probability of local tumor control and overall disease-free survival. factors that favor a good response to chemotherapy are tumor histology (well differentiated), location of the primary site (oral cavity and oropharynx), nodal status (n0) and size of the primary lesion (t3 better than t4). increased cellular maturation after chemotherapy correlates with an improved clinical response. the importance ...	1980	6158883
hemispheric potentials evoked by speech sounds during discrimination tasks.	we studied the feasibility of adapting the method of wood et al. (1971) for determining cerebral dominance for language and did not obtain consistent asymmetries of evoked responses in the left hemisphere to stop consonant task (sct) as compared with fundamental frequency task (fft). likewise, the right hemisphere-evoked potentials for sct and fft did not show consistent asymmetries. however, we did find that the evoked responses at t3 were attenuated as compared with those at t4, regardless of ...	1980	6159171
spatial distribution of somatosensory responses evoked by tapping the tongue and finger in man.	early components of the somatosensory potential (sep) evoked by tactile stimulation of the tongue were recorded from the scalp in 7 normal subjects and compared with those resulting from taps on the middle finger. (1) unipolar but not bipolar recording of sep to tapping the tongue failed to differentiate the early components from large eeg deflections of myogenic origin. no such contamination was seen in either unipolar or bipolar recording of sep following taps on the finger. (2) the early comp ...	1980	6159176
effect of streptozotocin diabetes on the hypothalamic-pituitary-thyroid axis in the rat.	studies of the hypothalamic-pituitary-thyroid axis have been performed in streptozotocin (stz)-diabetic wistar rats and their controls. plasma pbi concentration, plasma and pituitary tsh, contents, and hypothalamic trh content were measured by ria in basal and stimulated conditions. compared to controls, rats made diabetic by 6.0 or 7.5 mg stz/100 g bw showed decreased plasma pbi and tsh and diminished pituitary tsh content, with greater alterations in rats receiving the highest stz dose. both d ...	1980	6159207
[liver deiodase activity in rats of different body weight and fat contents following high or low fat diet. comparison of enzymatic deiodination with t4-clearance rate and thyroid activity in animals].	weanling male wistar rats were fed 4 weeks a standard diet and separated into 2 groups, which received a high fat diet (50% w fat; hfd) or a low fat diet (3% w fat; lfd). these diets were fed 6-8 weeks and the animals then separated into light and heavy animals in each group. t4 deiodination in liver homogenates was investigated in all groups and compared with t4 clearance rate and thyroidal activity of these animals. the hfd-rats showed independently of weight and body fat content significantly ...	1980	6159208
derivation of a monoclonal antibody that detects an ia antigen encoded by 2 complementing i-subregions.	a monoclonal antibody designated a303, which reacts with ia antigens controlled by the complementation of 2 distinct i-subregion genes, is described. complementation of both i-a and i-ec subregion gene products is necessary for the cytotoxic, direct and 125i-protein a-amplified indirect cell binding, and immunoprecipitation activities of a303. positive prototype strains of independent origin include h-2k,p, whereas a303-negative haplotypes include h-2b,d,f,r,s,u. seven recombinant strains, h-2ap ...	1980	6159401