Publications

head shell protein hoc alters the surface charge of bacteriophage t4. composite slab gel electrophoresis of phage t4 and related particles.		1980	7441750
partial replicas of uv-irradiated bacteriophage t4 genomes and their role in multiplicity reactivation.	a physicochemical study was made of the replication and transmission of uv-irradiated t4 genomes. the data presented in this paper justify the following conclusions. (i) for both low and high multiplicity of infection there was abundant replication from uv-irradiated parental templates. it exceeded by far the efficiency predicted by the hypothesis that a single lethal hit completely prevents replication of the killed phage dna: i.e., some dead phage particles must replicate parts of thier dna. ( ...	1980	7441816
suppressors of mutations in the bacteriophage t4 gene coding for both rna ligase and tail fiber attachment activities.	the protein product of t4 gene 63 catalyzes both the attachment of tail fibers to fiberless phage particles and the ligation of single-stranded rna (snopek at al., proc. natl. acad. sci. u.s.a. 74:3355-3359, 1977). to investigate whether the gene 63 product has a role in nucleotide metabolism, we isolated false revertants of amm69 in gene 63. we screened for revertants that could grow at 30 degrees c but not at 43 degrees c on escherichia coli ok305 when nucleotides were limiting. these false re ...	1980	7441817
severe hypertension in patients with high spinal cord lesions undergoing electro-ejaculation--management with prostaglandin e2.	intra-arterial blood pressure (bp) and heart rate (hr) were continuously recorded in five patients with spinal cord injuries at different levels who were undergoing electro-ejaculation. in three patients with lesions at c7, c5 and t4 insertion of the electrode and electrical stimulation caused severe hypertension and bradycardia. in a patient with a t7/8 lesion and in another with a t10 lesion there were either moderate or minimal cardiovascular changes. severe hypertension during electro-ejacul ...	1980	7443281
failure of low doses of 131i to ablate residual thyroid tissue following surgery for thyroid cancer.	thirteen patients received an initial dose of 25-29.9 mci (9259-1, 106 mb1) of 131i following partial thyroidectomy for papillary, follicular, or mixed carcinoma. administration of thyroxine (t4) or triiodothyronine (t3) was stopped 3-12 weeks and 1-6 weeks, respectively, before therapy or imaging. patients remained on normal diets and did not receive thyroid-stimulating hormone (tsh) or diuretics. follow-up 3 months to 2 years after therapy demonstrated that ablation of thyroid bed activity was ...	1980	7444061
the quantitation of trypanosoma congolense in calves. ii. biochemical changes.	changes in serum electrolytes, osmolality, total proteins and protein fractions, lipid levels and organ function tests were determined in neonatal and 6-month-old calves infected with t. congolense treu 112 and control calves. there were no consistent changes in electrolytes or osmolality for either age group of calves. there was a decrease in total serum protein in the infected 6-month-old calves, caused by decreases in the albumin and beta 2-globulin fractions. the total serum protein level re ...	1980	7445066
serum reverse triiodothyronine (rt3) in children with goitre.	serum reverse t3, t3, t4, and tsh concentrations were measured in children with goitre of grade ii and iii, and in controls of the same age. the average rt3 level was decreased in both goitrous groups and the difference between them was significant. the rt3/t4 ratio in each group changed in a similar way. it seems that in goitre not only the t3 secretion of the thyroid gland is increased but also the deiodination of t4 to t3 increases against rt3, or the degradation and metabolic clearance of t3 ...	1980	7446089
the cell cycle kinetics of human breast cancer.	cell kinetic parameters were determined in 32 patients with breast cancer following intravenous [3h]thymidine injection. the mean labelling index (li) was 8.6, range 1.2 to 24.1. the li increased significantly with increased stage of disease. the highest values were noted in patients with chest wall metastases with a mean li of 15.9. in one patient with multiple discrete tumor foci, the larger foci had significantly decreased li consistent with classic cytokinetic theory. the intralesional varia ...	1980	7448702
[fundamental and clinical evaluation of radioimmunoassay for serum thyroxine by t4 corning ria kit (author's transl)].		1980	7449163
[fundamental and clinical evaluation of spac t4 ria kit (author's transl)].		1980	7449164
[mechanisms of t4 hyperthyroidism].		1980	7433081
abnormalities of oxidative phosphorylation due to excess of deficiency of thyroid hormones.	atp synthesis requires the presence of thyroid hormones that activate oxidative phosphorylation. atp supports the energy consuming reactions such as actin-myosin interaction, calcium and sodium pump, which are essential in a normal heart function. thyroid insufficiency depresses mitochondrial oxidation, leading to atp depletion which can not be compensated by activated glycolysis. glycolysis under conditions of hypoxia due to accumulation of lactate or nadph, impairs membrane permeability and mi ...	1980	7433860
studies on the action of pesticides upon the endocrines using in vitro human thyroid cells culture and in vivo animal models. ii. herbicides-prometrin and terbutrin.	the effects of prometrin and terbutrin in doses of 0.001--1 mg/1.5 ml culture medium on human thyroid cell cultures were studied following up their influence on cell multipliction, proteic synthesis, enzymatic activity of thyroid cells and hormonal synthesis. the results differed in relation to the dose and the type of thyroid cell under study. the acute experiment performed on rats treated with 50 ppm/prometrin or terbutrin/kg b.w. for 15 days revealed variations in t3, t4 and lh synthesis. bot ...	1980	7433862
influence of bcg on the development of 256 walker carcinosarcoma inoculated intratesticularly in the rat, in relation to thyroid status alerations.	the effect of nonspecific antitumoral immunity caused by bcg on intratesticular development of 256 walker carcinosarcoma has been studied on male hyper- or hypothyroidectomized rats. thyroid hormones level was altered by administration of t4. alkiron or thyroidectomy. the time and route of bcg administration differed from one lot to another. authors' results show that administration of bcg before or concomitantly with the graft stimulates tumor development, whereas administration of bcg mixed wi ...	1980	7433863
[modification of thyroid hormones by unsaturated fatty acid after noradrenaline load].	in 13 patients with a hyperlipoproteinaemia of type iia under a diet fat-modified and rich in linoic acid which contained only few animal fats and cholesterol the long-term behaviour of t3 and t4 was determined. after 7 months significant reductions of the t3- as well as increases of the t4-concentrations in the blood were found, in which cases the physiological regions were neither transgressed nor subgressed. these results correspond to the results given in literature, established, however, in ...	1980	7434833
[effect of noradrenergic stimulation on the peripheral venous concentrations of thyroxine and triiodothyronine in young test subjects with normal metabolism].	the influence of intravenous noradrenalin infusions in increasing dosages lasting 60 minutes on the peripheral venous total t3- and total t4-concentrations of blood, blood pressure and heart rate was established on 5 test persons during a period of 110 minutes. immediately after the addition of 0.12 micrograms na/kg kgw./min an increasing tendency of the t4-concentration in the venous blood occurred, respectively. under influence of higher na-doses (0.20 and 0.33 micrograms/kg kgw./min) the nora ...	1980	7434834
serum reverse-t3 determinations in the laboratory diagnosis of hyperthyroidism.	the relative discriminatory value of the estimation of the serum reverse-t3 levels for the laboratory diagnosis of hyperthyroidism was investigated in 47 patients with clinical signs or symptoms of hyperthyroidism. the results were compared with those from the determination of the total serum levels of t3 and t4 prior and after correction for the binding proteins. twenty-three of the patients had normal thyroid function and 24 had hyperthyroidism. the estimation of the total serum t3 level was s ...	1980	7435255
maturation of hypothalamic-pituitary-thyroid response in the rat to acute cold.	groups of rats 3, 4, 5, 7, and 10 wk of age were tested for their ability to respond to acute cold (4 degrees c for 30 min). thyroid reactions were estimated from intrathyroidal colloid droplet (cd) counts, serum thyrotropin (tsh) and thyroxine (t4) levels. normally cd counts increased to a maximum in the 5-wk-old rats were lower in the 7-wk-old, and still lower in the 10-wk-old animals. the significant finding was that exposure to cold caused a decrease in cd counts in 3-, 4-, 5-wk-old rats but ...	1980	7435557
hypothermia in iron deficiency due to altered triiodothyronine metabolism.	iron-deficient rats become hypothermic and have an excessive catecholamine response when exposed to an ambient temperature of 4 degrees c. this is not due to changes in body insulation, since thickness is unaltered, since differences persist after removal of hair, and since cutaneous vasoconstriction is intact. on the other hand, oxygen consumption of iron-deficient animals at 4 degrees c is reduced, 39 +/- 3 ml . kg-1 . min-1 compared to 63 +/- 2 in control animals. thyroxine (t4) values at 4 d ...	1980	7435650
combined craniofacial resection for locally advanced carcinoma of the head and neck ii. carcinoma of the paranasal sinuses.	twenty-eight patients with t3 and t4 carcinomas of the paranasal sinuses underwent orbital exenteration and combined craniofacial resection of the anterior and middle cranial fossa and the soft tissues of the face. three patients died from the procedure and nine had nonfatal complications. seventy-two percent of the patients with carcinoma of the maxillary antrum are free of disease at 3 years and 50 percent are free of disease at 5 years.	1980	7435819
comparative absorption of colostral igg1 and igm in the newborn calf. effects of thyroxine, cortisol and environmental factors.	twelve holstein x friesian calves, born in the same stable, and then transferred to outside individual boxes, were used in this experiment. six animals received thyroxine (t4) injections at birth and after 24 h. all the calves received the same colostrum in the same conditions. the following results were obtained: --the capacity of calves to acquire colostral igg1 and igm varies considerably between individuals. --t4 injections do not noticeably alter igg1 and igm absorption, but thyroxinemia at ...	1980	7436325
low-iodine diet for producing iodine deficiency in rats.	a low-iodine diet has been prepared for rats, using locally available low-iodine ingredients. on analysis it has been shown to consistently contain 15-20 ng iodine/g. when fed to growing female rats, this diet produced severe iodine deficiency while not significantly affecting growth or reproduction. the deficiency was manifested by a fall in daily urinary iodine excretion (to less than 1 microgram/day) and a seven-fold increase in thyroid uptake (131i) observable within 3 months. levels of plas ...	1980	7436868
binding of reverse t3 to hepatic nuclear protein.	the study was undertaken to examine the potential intrinsic biological activity of reverse t3 by comparing its binding to nuclear protein in vitro with that of other iodothyronines. nuclear protein was extracted from normal pig liver using methods described for rat tissues. at 25 degrees 30% of the added 125i rt3 was specifically bound to the nuclear protein. the order of potency in displacing 125i rt3 was rt3 > 3'5't2 > t4 > t3 > 3,3't2 > 3,5t2. this contrasted with the order of potency in disp ...	1980	7436879
neonatal thyroid function and anaesthesia.	thyroid function in the neonate and its response to hexobarbitone anaesthesia were studied in newborn wistar rats between 24 h and 42 days old. 14c-labelled mixed with unlabelled hexobarbitone was injected i.p. in anaesthetic concentrations. serum t3, reverse t3 (rt3) and t4 and serum and whole brain hexobarbitone concentrations were measured on wakening. a decrease in serum t3 and rt3 and an increase in t4 concentrations were observed after hexobarbitone at 21 and 30 days. this appears to be a ...	1980	7437218
the straight back syndrome.	the straight back syndrome, consisting of loss of normal upper thoracic spinal curvature associated with cardiac murmurs and radiographic cardiomegaly is considered a form of 'pseudoheart disease' which has been attributed to squashing of the heart in the reduced ap diameter of the chest. during an 18-month period 31 patients referred to a cardiologist were found to have a straight back. forty-five relatives were subsequently examined and 27 were found also to have a straight back. palpitations ...	1980	7267961
scaffolding proteins and the genetic control of virus shell assembly.	historically a gap has existed between the study of the one-dimensional organization of hereditary information in genes, and of the three-dimensional organization of macromolecules in biological structures. in this article we describe progress in closing this gap through the genetic and biochemical analysis of the assembly of the icosahedral shells of spherical viruses, a class of subcellular structures whose subunit organization is relatively well understood. the genes specifying the proteins r ...	1980	7267974
effect of anticonvulsant and antidepressant drugs on iodothyronines in serum.	serum iodothyronine concentrations were measured in patients on long-term therapy with one or two anticonvulsant drugs. diphenylhydantoin (dph) and carbamazepine (cbz) reduced the serum levels of thyroxine (t4) free t4 index, reverse triiodothyronine (rt3) and 3,3'-diiodothyronine (3,3'-t2), whereas the depressant effect on the serum levels of triiodothyronine (t3) and free t3 index was small but statistically significant. in patients administered dph and phenobarbital (pb) or cbz and primidone ...	1980	7280555
amniotic fluid reverse triiodothyronine in normal and high risk pregnancies.	the authors have dosed by radioimmunoassay the rt3 concentrations in the amniotic fluid of 12 normal pregnant women and of 25 women with high risk pregnancies at the same period of gestation. the average of the rt3 concentrations in the amniotic fluid of pregnant women affected by diabetes mellitus, eph gestosis and with foetal growth, retardation, are not different from those found in the control group. in the patients affected by rh isoimmunization, the rt3 levels are remarkably higher than th ...	1980	7471456
lymphography in the evaluation of urinary bladder carcinoma.	lymphography was performed in 41 patients with bladder carcinoma. the lymphographic findings were correlated to the clinical and histological staging and the pre-operative grading. twenty-seven patients were operated upon. the findings of lymphography were compared with the findings at operation, and the pathological examination of the removed lymph nodes. the lymphographic diagnosis was proved to be correct in 77.7% of the patients. there were two false positive lymphographies, and one false ne ...	1980	7471630
the effect of iodothyronines on the conversion of thyroxine into 3,3'-5-tri-iodothyronine in isolated rat renal tubules.	isolated rat renal tubules prepared by collagenase digestion were used to study the effects of 3,3',5'-tri-iodothyronine ('reverse t3', rt3) and other iodothyronines on the formation of 3,3',5-tri-iodothyronine (t3) from thyroxine (t4). rt3 inhibited the conversion with a dose response over the concentration range 1.5nm-1.5microm. the inhibition was competitive in nature. both 3,3'-di-iodothyronine and 3',5'-di-iodothyronine also inhibited the production of t3 and t4 in isolated rat renal tubule ...	1980	7470050
[the primary empty sella syndrome. a case report study (author's transl)].	the empty sella results from an extension of the subarachnoid space into an intrasellar position with subsequent remodeling of the sella turcica and the flattening of the pituitary gland. the sella turcica is usually enlarged causing the greatest diagnostic difficulty to distinguish it from a pituitary tumor. the most patients with this syndrome usually have normal pituitary function, while about 30% have varying degrees of hypopituitarism. the authors describe a case report of a man with primar ...	1980	7470179
plasma corticosterone, thyroxine and triiodothyronine level in the collared dove (streptopelia decaocto) during the reproductive cycle.	male and female collared doves living in their natural environment, sexually inactive or in different stages of their reproductive cycle, were collected. plasma triiodothyronine (t3) and thyroxine (t4) were determined by radioimmunoassay (ria), whereas the corticosterone (crt) level was measured by competitive protein binding assay (cpba). the plasma t3 level showed no change except in female hatching birds. the t4 content was constant in young and sexually inactive birds, while in male birds it ...	1980	7282373
[thyroid function in patients with alcoholic cirrhosis (author's transl)].	total serum thyroxine (t4), total serum triidothyronine (t3), thyrotropin (tsh) and trh induced tsh release have been measured in 50 clinically euthyroid men with alcoolic cirrhosis and compared to 20 age matched control men in order to determine the thyroid status in cirrhosis. free serum thyroxin (ft4), free triiodothyronine (ft3) and reverse t3 (rt3), were measured in 34 patients. in these patients, a clinical and biological index was devised to score the severity of the disease. finally, thy ...	1980	7469382
[automation of t4 and tsh radioimmunoassay in the neonatal screening for hypothyroidism in south-west france (author's transl)].	in the south-west france the neonatal screening service for hypothyroidism using both t4 and tsh undertakes 50 000 assays annually. to obtain maximum reliability and speed the calculations and statistical analysis have been automated. counting is done by gamma counter linked through magnetic tapes to programmable computer. this provides immediate print-out of hormone levels and for each group of the results the mean and standard deviation of the t4 levels and histogram of the tsh values. the cou ...	1980	7469680
[management of primary hypothyroidism in childhood treated with thyroid extract (author's transl)].	in a group of 19 children with primary hypothyroidism who were treated with thyroid extract, linear relationships were found between blood thyroxine and triiodothyronine levels and the dose of thyroid extract adjusted for surface area. thus it is likely that the composition of thyroid extract is homogeneous and that the metabolism of thyroid hormones in the patients is similar. thyroxine (t4) was the most reliable parameter for monitoring treatment as t3 levels were elevated in patients who were ...	1980	7469681
[screening for neonatal hypothyroidism by measurement of both t4 and tsh in dried blood eluates (author's transl)].	systematic screening for neonatal hypothyroidism has been undertaken since january 1977 in southern france by the measurement of both t4 and tsh in dried blood eluates. 76,432 measurements were performed in 3 years and 22 cases of hypothyroidism were detected. there were several advantages of this method; detection of all forms of thyroid disease, immediate diagnosis of primary hypothyroidism with t4 < -2 s.d. and tsh > 80 microu/ml, decrease in the number of false positive results, detection of ...	1980	7469693
thyroid function tests during carbimazole therapy.	changes in plasma thyroxine (t4), triiodothyronine (t3), free thyroxine index (ft4i) and thyroid stimulating hormone were studied in 100 patients with graves' disease treated with carbimazole. during therapy plasma t3 concentrations were disproportionately high compared to those of t4, the t4 : t3 ratio was low, and many patients were clinically euthyroid with a normal plasma t3 but low t4 concentration. although there was considerable individual variation in response, the order of response was ...	1980	6894979
[relation between pulmonary surfactant and thyroid hormones (t4, t3) in the newborn infant].		1980	6894675
movement and self-control in protein assemblies. quasi-equivalence revisited.	purposeful switching among different conformational states exerts self-control in the construction and action of protein assemblies. quasi-equivalence, conceived to explain icosahedral virus structure, arises by differentiation of identical protein subunits into different conformations that conserve essential bonding specificity. mechanical models designed to represent the energy distribution in the structure, rather than just the arrangement of matter, are used to explore flexibility and self-c ...	1980	6894706
long-term results of radioiodine (131i) therapy in 331 patients with graves' disease.	to evaluate the long-term results of 131i treatment for graves' disease, the thyroid function was studied in 331 patients 5-17 years after this therapy. twenty-five patients were hypothyroid and had already been on thyroid medication. among the remaining 306 patients without any treatment for thyroid disorder, 188 patients (61.4%) had a normal thyroid-stimulating hormone (tsh) level (less than 10 microu/ml) of whom 151 were euthyroid with normal thyroxine (t4) and triiodothyronine (t3) level. 11 ...	1980	6894205
properties of the nonlethal recombinational repair deficient mutants of bacteriophage t4. iii. dna replicative intermediates and t4w.	the rate at which 3h thymidine is incorporated into dna is increased in t4w-infected cells compared to wild-type when measured late in infection under conditions of low thymidine concentration. this increased dna synthesis is sensitive to hydroxyurea but not to mitomycin c, and can be prevented by the addition of chloramphenicol early in infection. also, dna replicative intermediates isolated from t4w-infected cells late in infection sediment significantly faster than those isolated from wild-ty ...	1980	6929402
recombinational repair of mitomycin c lesions in phage t4.		1980	6930130
pentaribonucleotides of mixed sequence are synthesized and efficiently prime de novo dna chain starts in the t4 bacteriophage dna replication system.	in the presence of single-stranded dna, the bacteriophage t4 gene 41 and gene 61 proteins catalyze the synthesis of a group of pentaribonucleotides which are homogeneous in chain length but heterogeneous in nucleotide sequence. when single-stranded t4 dna is used as template, a unique dinucleoside sequence, pppapc, is found at the 5' end of these pentaribonucleotides with the general sequence pppapcpnpnpn. in the presence of the remaining five t4 replication proteins, the pentaribonucleotides ca ...	1980	6934503
[morphologico-genetic study of the enzymology of t4 phage recombination. ii. effect of antimutational t4 dna-polymerase on correction-type recombination].	effect of temperature antimutator l42 allele of gene 43 of t4 phage on the parameters of correction-type mechanism of genetic recombination have been studied under permissive conditions. the effect of l42 was found to be marker- as well as distance-dependent, and the pattern of these dependences shows that in the presence of antitumor dna polymerase the distribution of the length of the repair region becomes more sharp and the mean length of the repair region becomes shorter. from this, it is co ...	1980	6935150
[maxillary cancer. a retrospective study of 239 cases (author's transl)].	a study of the distribution of these cancers demonstrated that they were mainly epidermoid carcinomas (144 cases), cylindromas (38 cases), and sarcomas. the series included 58% of male patients, but the percentage of women is high because of the predominance of muco-epidermoid epitheliomas and hematosarcomas in female patients. age distribution was identical for both sexes. the period between the first sign and the diagnosis varied according to the histological type (5 months for epidermoid carc ...	1980	6935735
caffeine as a comutagen for ethylmethanesulfonate in strains of phage t4.	the mutation frequency of dna polymerase mutants of phage t4 treated with ethyl methanesulfonate (ems) then incubated in the presence and absence of caffeine was studied using an rii reversion system. the dna polymerase mutation is shown to be antimutagenic for ems induction of reversions which occur by a gc to at transition. caffeine acts as a comutagen for the induction by ems of mutant phages and produces a significant increase in the frequency of reversions from rii to r+. caffeine is slight ...	1980	6936594
phenotypic differences among the alleles of the t4 recombination defective mutants.	the allelic forms of the phage genes t4 chi and fdsa, as well as t4y and fdsb are compared in terms of their thymidine incorporation in high or low concentrations of thymidine, sensitivity of dna synthetic capacity to mitomycin c, and sedimentation rates of dna replicative intermediates. the results show differences among these mutants for the incorporation of thymidine; however all exhibit mitomycin c-sensitive dna synthesis and have identical aberrant sedimentation rates for their dna replicat ...	1980	6936596
high negative interference and recombination in bacteriophage t5.	the process of close recombinant formation in bacteriophage t5 crosses has been studied by examining the structure of internal heterozygotes (hets), the immediate products of recombination events. the t5 system was chosen because it permits the study of internal heterozygotes exclusively, thus avoiding the ambiguities inherent in previous studies with t4. the heterozygotes were obtained by the nonselective screening of progeny phage in a prematurely lysed sample from an eight-factor cross. the m ...	1980	6937420
release mechanisms of postoperative changes in various acute phase proteins and immunoglobulins.	postoperative changes in various acute phase proteins (transferrin, albumin, prealbumin, orosomucoid, haptoglobin, iga, igg, and igm), plasma glucose, and cortisol were studied in twelve otherwise healthy premenopausal women undergoing elective hysterectomy during either general anaesthesia or epidural analgesia (t4-s5). the results showed a 10-20% decrease of all proteins during the first postoperative day (p less than 0.05), followed by a gradual, significant increase of orosomucoid to 80% and ...	1980	6941601
effect of propylthiouracil on 125i-l-triiodothyronine binding to the nuclei and on malic enzyme activity in rat liver cytosol.	the effect of propylthiouracil (ptu), triiodothyronine (t3) and thyroxine (t4) on malic enzyme activity of rat liver cytosol and on the binding of 125i-l-triiodothyronine to nuclear fraction was examined. significant decrease of in vitro binding of 125i-t3 to the liver nuclei was found in rats fed ptu for 12 h, 24 h and 120 h. hepatic malic enzyme activity was unchanged after 12 h, but markedly decreased after 24 h and 120 h of ptu treatment. in 120 h ptu fed animals the effect of t3 on malic en ...	1980	6967013
biochemical analysis of human t lymphocyte differentiation antigens t4 and t5.	two major functionally distinct t cell subsets in man have been defined with heteroantiserums and monoclonal antibodies directed against stable cell surface antigens that appear during thymic ontogeny. a monoclonal antibody to t4 antigen (anti-t4) is reactive with the peripheral inducer t cell population while a monoclonal antibody to t5 antigen (anti-t5) is reactive with the cytotoxic and suppressor population. immunoprecipitation and electrophoresis on sodium dodecyl sulfate polyacrylamide gel ...	1980	6967228
serum thyroxine, triiodothytonine and reverse triiodothyronine in early postnatal period in pig (preliminary communication).	the rapid increase in serum thyroxine (t4), triiodothyronine (t3) and reverse triiodothyronine (rt3) in the pig shortly birth presumably due to enhanced secretory activity of the thyroid gland was observed. during the 2nd and 3rd week of live t3 and t4 remained essentially unchanged but rt3 continued to rise to a high plateau level. this resulted in an increase in rt3/t4 ratio to about two and rt3/t3 raio to about one. a shift to rt3 production seems to be a reflection of an increased capacity o ...	1980	6967403
regulation of b cell immunoglobulin secretion by functional subsets of t lymphocytes in man.	two distinct immunoregulatory t cell subsets, termed t4+ and t5+, have been defined in man by monoclonal antibodies. prior studies have shown that the t4+ t cell population provided help for b cell immunoglobulin (ig) production and was required for generation of t5+ cytotoxic effector cells. in the present study, the regulatory effects of the t5+ t cell subset on b cell ig secretion were determined in a pokeweed mitogen-driven system. it was found that the t5+ subset, in contrast to the t4+ sub ...	1980	6967817
the effects of whole-body irradiation on the serum levels and kinetics of thyroid hormones in rats.	the effects of a single whole-body dose of x-rays on the serum levels and kinetics of thyroid hormones in rats were studied. the influence of radiation-induced anorexia was monitored by using pair fed control groups. a dose of 800 rad caused a reduction in t4 levels and 750 rad had a similar effect on t3; in each case the control group showed a smaller reduction. the kinetic results indicated that, in the control groups, the early reduction in hormone concentrations was caused, by a change in th ...	1980	6968299
fc receptor-bearing blood mononuclear cells in thyroid disorders: increased levels in patients with subacute thyroiditis.	blood mononuclear cells bearing fc receptors for immunoglobulin g were measured in patients with thyroid disorders as the percentage of ea rosette-forming cells (% ea-rfc). levels were normal in patients with untreated graves' hyperthyroidism, graves' ophthalmopathy, and hashimoto's thyroiditis. on the other hand, the % ea-rfc was increased in eight of nine patients with subacute thyroiditis (sat) tested during the acute phase, returning to normal during recovery. levels were normal in all five ...	1980	6968319
action of thyroid hormones in premetamorphic tadpoles: an important role for thyroxine?	the role of t4 as an active hormone in premetamorphic tadpoles has been investigated. it was found that the only labeled compounds present in serum and preparations of liver cytosol and nuclei 1, 3, and 24 h after injection of [125i]t4 and [125i]t3 (10 pmol/tadpole) were iodide, the injected hormone, and a trace of labeled material that remained at the origin during chromatography (origin material). although t4 was deiodinated more rapidly than t3, no conversion of t4 to t3 was detected. similar ...	1980	6968674
acute effect of salicylate on t4 and t3 levels in plasma and their excretion by bile in rats.	male wistar rats weighing about 400 g were anaesthetized with pentobarbiturate (40 mg kg-1) i.p. and thin polyethylene tubings were inserted in left femoral artery and vein as well as in a bile duct. blood samples were taken at 30-60 min intervals for 6 h. the total bile was collected to pre-weighed glass vials. with the aid of radioimmunoassay the level of thyroxine (t4) and 3,5,3'-triiodothyronine (t3) was estimated in plasma and the total excretion of these compounds by bile was measured afte ...	1980	6969174
the rapid purification of t4 dna ligase from a lambda t4 lig lysogen.	a procedure has been developed for the rapid purification of the enzyme t4 dna ligase. the procedure involves the induction at 42 degrees c of a lambda lysogen containing the gene for t4 dna ligase (murray, n.e., bruce, s.a., and murray, k. (1979) j. mol. biol. 132, 493-504), followed by purification of the ligase activity by phosphocellulose and hydroxylapatite chromatography. this results in the purification of large amounts of ligase with very high specific activity. the enzyme is free of con ...	1980	6985906
role of bacteriophage t4 dna-delay gene products in deoxyribonucleotide synthesis.		1980	6986383
primary structure of escherichia coli trna uur leu. presence of an unknown adenosine derivative in the first position of the anticodon which recognizes the uu codon series.	the primary structure of escherichia coli trna uur le which recognizes the uu series of codons has been determined. the sequence is pg-c-c-c-g-g-a-s4u-g-g-u-g-g-a-a-d-c-gm-c-d-a-g-a-c-a-c-a-a-g-g-g-a-psi-u-n-a-a-ms2i6a-a-psi-c-c-c-c-u-c-g-g-c-g-g-c-g-u-u-c-g-c-g-c-u-g-u-g-c-g-g-g-t-psi-c-a-a-g-u-c-c-c-g-c-u-c-c--g-g-g-u-a-c-c-a. the chain length of trna uur leu is 87 residues, the same as other e. coli trna leu s and t4 phage-coded trna leus. its sequence is especially similar to that of e. coli ...	1980	6986390
t4 ribonucleic acid ligase joins single-strand oligo(deoxyribonucleotides).	t4 rna ligase joins a 3'-hydroxyl-terminated acceptor oligoribonucleotide to a 5'-phosphate-terminated donor oligoribonucleotide. an analogous reaction with single-strand dna oligonucleotides would be useful for the synthesis of defined sequences of dna because it would eliminate the need to synthesize complementary sequences to form the duplex substrates required by dna ligase. we have studied the model reaction da(pda)5 + [5'-32p] (pdt)4pdcp leads to da(pda)5 [3' leads to 5'-32p]pdt(pdt)3pdcp ...	1980	6986903
alterations in the cell envelope of escherichia coli late in bacteriophage t4 infection.	the cell envelope of escherichia coli was examined for changes during late stages of bacteriophage t4 infection. late events in t4 infection are shown to result in (i) a reduction in the effectiveness of membrane separation procedures employing either isopycnic sucrose gradient centrifugation or selective solubilization of inner membrane by detergent (sarkosyl or triton x-100), (ii) the appearance of a 54 000 dalton host protein in membrane preparations, (iii) the adventitious presence of deterg ...	1980	6986912
effect of bacteriophage t4 nrd mutants on deoxyribonucleotide synthesis in vivo.		1980	6987228
circulating lh, fsh, t, prolactin, tsh, t3, t4, gh and insulin in pubertal females.		1980	6987726
role of the host cell in bacteriophage t4 development. i. characterization of host mutants that block t4 head assembly.	to study the role of the host cell in bacteriophage t4 infection, we selected more than 600 mutant host-defective bacteria that absorbed and were killed by phage t4+ but were unable to support its growth. the mutants were grouped into seven classes by the growth patterns of t4 phages carrying compensating mutations (go mutants [grows on]), selected on four prototype host-defective strains. lysis and dna synthesis experiments indicated that classes a, ad, d, and b (the majority of the host-defect ...	1980	6988606
somatomedin activity and growth hormone secretion. i. changes related to body weight in anorexia nervosa.	anorexia nervosa in childhood and adolescence, associated with impaired growth can be considered as a form of chronic malnutrition. twelve patients aged 11 to 17 years were investigated. in spite of increased gh secretion, plasma somatomedin activities were diminished in 8 cases. plasma t4, t3 and arginine stimulated insulin secretion were also decreased. in six patients who gained weight a significant negative correlation was found between weight deficit and plasma somatomedin activity. prolong ...	1980	6989150
altered bacteriophage t4 ribonucleic acid metabolism in a ribonuclease ii-deficient mutant of escherichia coli.	some early t4 ribonucleic acids were not found in an infected ribonuclease ii-deficient strain but were formed in ribonuclease ii+ transductants as well as in wild-type escherichia coli.	1980	6989808
efficient in vitro replication of double-stranded dna templates by a purified t4 bacteriophage replication system.	a wide variety of double-stranded dna templates are replicated extensively in an in vitro dna replication system containing the purified proteins specified by seven t4 bacteriophage dna replication genes (32, 41, 43, 44, 62, 45, and 61). in favorable conditions, this multiprotein system catalyzes the synthesis of several copies of the input dna template in a 30- to 60-min incubation. the replication forks produced in vitro move in a highly processive fashion, at approximately the in vivo rate of ...	1980	6989836
mutants of bacteriophage t4 that produce infective fibreless particles.		1980	6990005
roles of bacteriophage t4 gene 5 and gene s products in cell lysis.	previous studies indicated that (i) t4 gene s product (gps) protects infected cells from superinfection lysis from without, (ii) the absence of gps in infected cells also leads to lysis from within even when t4 e lysozyme is absent, (iii) t4 gene 5 product (gp5), a polypeptide of the virion baseplate, may be responsible for inducing lysis from without, and (iv) altered gp5 of the t4 mutant 5ts1 can replace e lysozyme to cause lysis from within. results of this study showed that (i) wild-type gp5 ...	1980	6990015
baseplate protein of bacteriophage t4 with both structural and lytic functions.	analyses of a new bacteriophage t4 mutant that permits lysis of infected cells in the absence of e lysozyme showed that the strain carried a suppressor mutation in gene 5, a gene whose polypeptide product (gp5) is an integral component of the virion baseplate. indirect experiments indicated that cell lysis was caused by the lytic action of mutant gp5. with regard to the physiological role of normal gp5, we speculate that it functions in the initiation of infection by catalyzing local cell wall d ...	1980	6990017
specific labeling of 3' termini with t4 dna polymerase.		1980	6990190
induction of mutations in specific genes of bacteriophage t4 using cloned restriction fragments and marker rescue.	saturation of a specific region of a chromosome with conditional lethal mutations becomes increasingly difficult as the genome size of the organism increases. we show that mutagenesis of cloned genes followed by their re-integration into a non-mutagenized organism is a practical way to circumvent this difficulty.	1980	6990202
inherited, early onset, insulin-requiring diabetes mellitus of keeshond dogs.	spontaneous diabetes mellitus has been characterized in a line of nonobese purebred keeshond dogs as an insulin-requiring hereditary disorder with onset at between 2 and 6 mo of age. diabetic dogs developed cataracts, became ketotic, hyperglycemic, hypercholesterolemic, lipemic, and hypoinsulinemic. basal glucagon, cortisol, and t4 serum concentrations and responses to acth, tsh, and arginine were normal. light microscopic studies of the pancreas by immunocytochemical procedures revealed the abs ...	1980	6991341
interactions of competent streptococcus sanguis (wicky) cells with native or denatured, homologous or heterologous deoxyribonucleic acids.	competent cell-deoxyribonucleic acid (dna) interactions were examined using tritium-labeled homologous or heterologous native or denatured dnas and competent streptococcus sanguis wicky cells (strain we4). the dnas used were extracted from we4 cells, escherichia coli b cells, and e. coli bacteriophages t2, t4, t6, and t7. the reactions examined were: (i) total dna binding, (ii) deoxyribonuclease-resistant dna binding, and (iii) the production of acid-soluble products from the dna. optimal temper ...	1980	6991480
isolation and characterization of context mutations affecting the suppressibility of nonsense mutations.	secondary mutations which increase the efficiency of suppression of nonsense mutations in the riib cistron of bacteriophage t4 have been isolated. these secondary mutations, called context mutations, map at sites very close to the nonsense codon, possibly on the promotor distal side. in context-nonsense double mutants, the amount of suppressed gene product is increased approximately 10-fold. the context mutations examined can act on the uaa (ochre) nonsense allele as well as on the uag (amber) n ...	1980	6991868
transcription termination factor rho and t-even phage development.	a functional factor rho is necessary for t-even phage development; phages t2 and t4 require different degrees of rho activity. the rho inactivation by ts-mutations in e. coli causes a reduction of some early protein synthesis and an early formation of some proteins normally typical of a later stage. besides, it weakens the synthesis of some late proteins, impairs the capsid proteins maturation and sharply inhibits phage dna replication in infected cells. however, in the absence of a functional r ...	1980	6991874
restriction in vivo. v. introduction of sos functions in escherichia coli by restricted t4 phage dna, and alleviation of restriction by sos functions.	degradation products of restricted t4 dna induced filamentation, mutagenesis, and to a lesser extent, synthesis of reca protein in wild type cells but not in reca, lexa or recbc mutants of escherichia coli. we conclude that the structural damage to the dna caused by restriction cleavage and exonuclease v degradation can induce sos functions. degradation of restricted nonglycosylated t4 dna by exonuclease v delayed cell division and induced filament formation and mutagenesis in lexa+ but not in l ...	1980	6991879
mutations in a nonessential viral gene permit bacteriophage t4 to form plaques on escherichia coli vals ts rela.	during viral development bacteriophage t4 modifies the valyl-transfer rna synthetase of its host escherichia coli, but the function of the modification has remained elusive. a strain of escherichia coli has now been identified which is nonpermissive for wild-type bacteriophage t4, but permissive for bacteriophage mutants impaired in the modification reaction. a comparison with other bacteria suggests that nonpermissiveness is due to synthesis of a thermolabile valyl-transfer rna synthetase and r ...	1980	6992274
a membrane potential threshold for phage t4 dna injection.		1980	6992774
a mutant of e. coli that restricts growth of bacteriophage t4 at elevated temperatures.	after nitrosoguanidine mutagenesis, a phage host defective (phd) mutant of e. coli hfrh was isolated that supported the growth of t4d wild-type bacteriophage at 30 degrees, but not at 40 degrees or higher. eleven independent spontaneous mutants of t4 (go mutants) were isolated that overcame the growth restriction at high temperature. all of these mutants were located within three percent recombination of a gene 39 amber mutation in the clockwise direction on the standard map. in mixed infections ...	1980	6993283
isolation and characterization of dna fragments containing the dihydrofolate-reductase gene of coliphage t4.	dna of a mutant of the bacteriophage t4, which contains cytosine instead of glucosylated hydroxymethylcytosine, was shown to direct the synthesis of enzymatically active dihydrofolate reductase in a coupled in vitro transcription-translation system. the dna-directed synthesis of the enzyme was used to localize the dihydrofolate-reductase gene frd on a 2300 bp long restriction-nuclease-generated dna fragment. fine structure mapping showed that the gene is encoded on a segment of less than 1850 bp ...	1980	6993288
neighbour and temperature effects on base-analogue-induced mutation in phage t4.	the effects of neighbouring base pairs and of temperature on mutation frequencies were measured at nonsense sites in the t4rii region. 2ap-induced at leads to gc transition frequencies are insensitive to nearest-neighbour effects, while 5bu-induced ones are promoted by gc neighbours on the 5' side. the effect of temperature on 2ap- and 5bu-induced mutation frequencies shows no simple dependence on nearest neighbours. these results are incompatible with a unitary mechanism as explanation for the ...	1980	6993847
isolation and genetic properties of a bacteriophage t4 uvsx mutant.	a technique for isolating uv-sensitive mutants of bacteriophage t4 and a relatively simple method for localizing the uv-sensitive mutations on the genetic map are described. of 13 mutants isolated 5 were shown to be den v mutants and 1 was shown to be a uvsx mutant. of the remainder, 4 are probably uvsx mutants (based on their map location) while the indentity of the remaining 3 has not been determined. the uvsx mutant (uvsx102) had enhanced uv and gamma-ray sensitivity compared to the only othe ...	1980	6993926
homologous pairing in genetic recombination: formation of d loops by combined action of reca protein and a helix-destabilizing protein.	escherichia coli single-strand binding protein (ssb) or phage t4 gene 32 protein reduced the amount of reca protein required to catalyze the formation of d loops from double-stranded dna and homologous single-stranded fragments. neither ssb nor gene 32 protein alone catalyzed the formation of d loops, and excessive amounts of either protein, amounts that were sufficient to saturate the single strands, inhibited the formation of d loops completely. both the stimulatory activity and the inhibitory ...	1980	6994103
protein chemical approaches for the investigation of t4-head morphogenesis.		1980	6994830
effect of thyroxine on the immune response of mice in vivo and in vitro.	the effect of thyroxine on the immune response of balb/c mice to sheep erythrocytes was investigated. in mice rendered hyperthyroid by subcutaneous injections of t4 the primary immune response to an injection of srbc in vivo did not show a consistent increase in splenic anti-srbc plaque-forming cells. however, the total number of splenic cells in t4-treated mice was generally decreased, and thus, the number of pfc per 10(6) splenic cells in t4-treated mice was higher than those of saline and buf ...	1980	6995277
t4 ribonucleotide reductase. physical and kinetic linkage to other enzymes of deoxyribonucleotide biosynthesis.	this laboratory has described a multienzyme aggregate from t4 phage-infected escherichia coli which seems to participate in deoxyribonucleotide biosynthesis and efficient delivery of dna precursors to the replication apparatus. this paper describes improved methodology for isolation of this aggregate, and we present three lines of evidence supporting a role for ribonucleoside diphosphate reductase in functioning of the presumed complex. 1) ribonucleoside diphosphates are readily incorporated int ...	1980	6995453
[effect of escherichia coli mutations affecting the transcription termination rho on the development of t-even phages].	the functionally active transcription termination factor rho is necessary for the t-even phages development. the inactivation of the factor by thermosensitive e. coli gene rho mutations results in blocking phage development. it should be noted that phages t2 and t4 require different levels of this factor's activity. the synthesis of some early proteins diminishes during factor rho inactivation and a number of proteins typical for a later stage are observed early in infection. under the condition ...	1980	6995827
afterloading interstitial implant in head and neck cancer.	seventy-nine patients with head and neck malignancies were treated using afterloading interstitial iridium 192 techniques at los angeles county-university of southern california medical center and southern california cancer center from feb 1, 1974 to nov 30, 1975. twelve of the 13 t1 and t2 lesions and 17 of 25 t3 and t4 lesions remain locally controlled to a minimum follow-up period of 24 months. surgical salvage in case of treatment failure is possible with low morbidity because of relatively ...	1980	6996657
electrochemical h+ gradient but not phosphate potential is required for escherichia coli infection by phage t4.		1980	6997076
characterization of t4 double-stranded rna by equilibrium centrifugation in cs2so4- and alkaline iodide gradients.		1980	6997083
amino acid sequence studies on t4 gene 32 dna-binding proteins.	the amino acid sequence of a total of 281 residues (85%) out of 333 residues from t4 gene 32 protein has been determined. the unusual characteristics of the amino-terminal a and carboxyl-terminal b regions are discussed in relation to their possible roles in dna replication and genetic recombination.	1980	6997174
a second external quality control survey (eqcs) for serum triiodothyronine (t3) and thyroxine (t4) assays using the "munich model".		1980	6997427
bacteriophage t4 gene transcription studied by hybridization to cloned restriction fragments.		1980	6997494
the function of tail fibers in triggering baseplate expansion of bacteriophage t4.		1980	6997499
head maturation pathway of bacteriophages t4 and t2. v. maturable epsilon-particle accumulating an acridine-treated bacteriophage t4-infected cells.	a maturable head-related particle of bacteriophage t4 has been identified and characterized. this epsilon-particle has the same size as the prehead, but its shell is made of the cleaved product of gene 23 (gp23*). it contains internal matter, most likely the processed core proteins, which is lost or modified by experimental manipulations. it accumulates, together with partially filled ("grizzled") heads, in t4 infected cells that are treated with 9-aminoacridine. on sections of "well-preserved" ...	1980	6997509
role of the host cell in bacteriophage t4 development. ii. characterization of host mutants that have pleiotropic effects on t4 growth.	mutant host-defective escherichi coli that fail to propagate bacteriophage t4 and have a pleiotropic effect on t4 development have been isolated and characterized. in phage-infected mutant cells, specific early phage proteins are absent or reduced in amount, phage dna synthesis is depressed by about 50%, specific structural phage proteins, including some tail and collar components, are deficient or missing, and host-cell lysis is delayed and slow. almost all phage that can overcome the host bloc ...	1980	6999171
[study of the kinetics of the reaction reuniting dna fragments catalyzed by dna-ligase. case of homogeneous molecules].	the kinetics of different dna forms formation were studied during the ligation reaction. reaction was carried out using as a model bamh1 dna fragments of plasmid pbr322 and dna-ligase of bacterophage t4. the products of the reaction were separated with 1% agarose gel, followed by dna quantitation in the corresponding bands by scanning fluorimeter. the kinetics of the ligation reaction were measured in the range of dna concentrations from 1 to 100 micrograms/ml. the system of differential equatio ...	1980	6999328
alterations in immunoregulatory t cell subsets in active systemic lupus erythematosus.	to determine whether imbalance among subsets of human t cells exists in patients with systemic lupus erythematosus (sle), we analyzed peripheral blood lymphocytes in sle patients during active and inactive stages of disease. for this analysis, we used monoclonal antibodies to the surface antigens of inducer (t4) and suppressor (t5/t8) t cell subsets, as well as a common t cell antigen (t3). in contrast to normal and inactive sle patients, the percentage of t3+ cells was reduced in all active sle ...	1980	7000827