Publications

uptake of 111in-z2d3 on spect imaging in a swine model of coronary stent restenosis correlated with cell proliferation.	small targets such as cell proliferation in the coronary arteries may potentially be detected with single-photon imaging using high-radiotracer-specific activity. we hypothesized that an antibody linked to polymers to increase specific radioactivity can be visualized on spect images and that counts in the target will correlate with the strength of the biologic signal.	2004	14960651
adenoviral catheter-mediated intramyocardial gene transfer using the mature form of vascular endothelial growth factor-d induces transmural angiogenesis in porcine heart.	it is unclear what is the most efficient vector and growth factor for induction of therapeutic vascular growth in the heart. furthermore, the histological nature of angiogenesis and potential side effects caused by different vascular endothelial growth factors (vegfs) in myocardium have not been documented.	2004	14967735
laparoscopic partial nephrectomy using holmium laser in a porcine model.	to evaluate the utility of the holmium laser for partial nephrectomy in a porcine model.	2004	14974664
comparative chromosome painting between the domestic pig (sus scrofa) and two species of peccary, the collared peccary (tayassu tajacu) and the white-lipped peccary (t. pecari): a phylogenetic perspective.	the suidae and the dicotylidae (or tayassuidae) are related mammalian families, both belonging to the artiodactyl suborder suiformes, which diverged more than 37 million years ago. cross-species chromosome painting was performed between the domestic pig (sus scrofa; 2n = 38), a representative of the suidae, and two species of the dicotylidae: the collared peccary (tayassu tajacu; 2n = 30) and the white-lipped peccary (t. pecari; 2n = 26). g-banded metaphase chromosomes of the two peccaries were ...	2004	15218266
load dependence of cardiac output in biventricular pacing: right ventricular volume overload in pigs.	previous work from our laboratory has demonstrated that optimization of biventricular pacing is load dependent. during acute pulmonary stenosis and right ventricular pressure overload in swine, cardiac output was maximized by pacing the right ventricle 40 ms before the left ventricle. to extend those studies, this experiment examined biventricular pacing optimization during right ventricular volume overload.	2004	15224027
systematic reaction to electrolytic treatment of pig livers in vivo.	only a minority of secondary liver tumours are amenable to segmental resection and as a result, considerable research has been focused on developing ablative methods to destroy liver metastases. many of these methods are limited by the development of a systemic inflammatory response mediated by cytokines such as interleukin-8 (il-8) and tumour necrosis factor alpha (tnf-alpha). the aim of the present study was to determine if a systemic reaction occurred following electrolytic treatment of pig l ...	2004	15230798
comparison of systemic responses of radiofrequency ablation, cryotherapy, and surgical resection in a porcine liver model.	the degree of systemic response after hepatic radiofrequency ablation (rfa) has not been well investigated.	2004	15231521
methylprednisolone enhances contraction of porcine femoral head epiphyseal arteries.	the pathomechanism of steroid-induced femoral head necrosis is thought to be disturbed femoral head blood flow. we hypothesize that methylprednisolone increases vasocontraction of femoral head epiphyseal arteries, thereby reducing femoral head blood flow. nine immature female domestic pigs were randomly selected from a group of 18 to receive 24-hour methylprednisolone treatment, whereas the nine remaining pigs received the placebo control in a blinded fashion. after sacrifice, lateral epiphyseal ...	2004	15232435
closure of the cystic duct during laparoscopic cholecystectomy with a new feedback-controlled bipolar sealing system in case of biliary obstruction--an experimental study in pigs.	the excellent results of new devices like the new bipolar feedback-controlled sealing system (ligasure) for closure of blood vessels encouraged surgeons to use these instruments for other structures like bile ducts. the aim of this study was to evaluate the feasibility of closure of cystic duct in case of biliary obstruction.	2004	15239216
treatment of hypotension in pigs with an inspiratory impedance threshold device: a feasibility study.	an inspiratory impedance threshold device was evaluated in spontaneously breathing animals with hypotension to determine whether it could help improve systemic arterial pressures when fluid replacement was not immediately available.	2004	15241102
heterosis and epistasis for teat number and fluctuating asymmetry in crosses between jiaxing and iberian pigs.	in the framework of wright's view of evolution, long-separate breeds of domestic animals could establish different adaptive epistatic genetic complexes that could be destroyed in crossbred animals by recombination. the objective of this study was to evaluate heterosis and recombination effects in a crossing experiment involving two distinct european and asian breeds (iberian and jiaxing) in the f1 and two successive backcrosses to the iberian line. teat number (tn) was recorded in the right and ...	2004	15241457
a slaughter slab survey for extra-intestinal porcine helminth infections in northern tanzania.	a survey on extra-intestinal porcine helminths was conducted at three slaughter slabs that receive pigs from mbulu, a district endemic for porcine cysticercosis in northern tanzania. seventy carcases of pigs between 1 and 2 years old were examined between december 1997 and march 1998. the examination involved ante-mortem lingual examination for taenia solium cysticercosis followed by post-mortem inspection. in addition, a laboratory procedure was performed to determine whether any of these domes ...	2004	15241967
in vitro culture of epithelial cells from the caput, corpus, and cauda epididymis of sus domesticus.	this work describes a protocol to culture epididymal epithelial cells from the caput, corpus, and cauda regions of sus domesticus. epididymal epithelial fragments were obtained by dissection and enzymatic digestion with collagenase. about 30 epididymal fragments from each epididymal region were cultured in 24-well culture plates with supplemented rpmi-1640 medium at 37 degrees c, 5% co2 in air, and 100% humidity. a confluent monolayer of polygonal and tightly packed epithelioid cells from the th ...	2004	15251244
ureteral replacement using small-intestinal submucosa and a collagen inhibitor in a porcine model.	small-intestinal submucosa (sis) has been successful as an onlay graft in ureteral repair, but tubularized segment interposition of sis has been unsuccessful. our objective was to evaluate whether a type i collagen inhibitor, halofuginone, would prevent stricture formation in tubularized sis interposition.	2004	15253833
phosphorus runoff from incorporated and surface-applied liquid swine manure and phosphorus fertilizer.	excessive fertilization with organic and/or inorganic p amendments to cropland increases the potential risk of p loss to surface waters. the objective of this study was to evaluate the effects of soil test p level, source, and application method of p amendments on p in runoff following soybean [glycine max (l.) merr.]. the treatments consisted of two rates of swine (sus scrofa domestica) liquid manure surface-applied and injected, 54 kg p ha(-1) triple superphosphate (tsp) surface-applied and in ...	2004	15254136
trichinellosis associated with bear meat--new york and tennessee, 2003.	trichinellosis is a parasitic infection caused by tissue-dwelling trichinella roundworms and is associated traditionally with ingestion of pork from infected domestic swine. as a result of improvements in swine production, trichinellosis has declined steadily in the united states. however, infection also can result from eating the meat of wild animals. during 1997-2001, a total of 72 cases of trichinellosis (median: 12 cases annually; range: 11-23 cases) were reported to cdc; the majority of the ...	2004	15254452
the epidemiological investigation of trichinella infection in brown rats (rattus norvegicus) and domestic pigs in croatia suggests that rats are not a reservoir at the farm level.	whether the brown rat (rattus norvegicus) is a reservoir of trichinella spp. infection or merely an accidental host, which may be vector of trichinella spp., continues to be debated. we estimated the prevalence of trichinella sp. infection in brown rat populations and in domestic pigs in 2 villages in croatia, where trichinella sp. infection in pigs has been endemic in the past 10 yr. trichinella spiralis larvae, identified by a multiplex polymerase chain reaction analyses, were the only species ...	2004	15270124
monoclonal antibody-based immunohistochemical diagnosis of malaysian nipah virus infection in pigs.	formalin-fixed, paraffin wax-embedded tissues of three malaysian farm pigs naturally infected with nipah virus were used to investigate the value of anti-nipah virus mouse monoclonal antibodies (mabs) and rabbit polyclonal antibody for immunohistochemical diagnosis. mabs 11f6 and 12a5 gave intense immunolabelling in lung tissue that had been fixed in 10% neutral buffered formalin for about 4 years, whereas the reactivity of mabs 13a5 and 18c4 and polyclonal antibody was reduced significantly by ...	2004	15276859
in vivo porcine intradiscal pressure as a function of external loading.	spinal loading during daily activity as it relates to the ability of the intervertebral disc to sustain its integrity has been a major issue in spinal research. the purpose of this investigation was to establish the relationship between the intervertebral disc pressure in the nucleus and the load applied to the motion segment in an in vivo porcine model.	2004	15280761
effects of vasopressin on left anterior descending coronary artery blood flow during extremely low cardiac output.	because of the possibility of vasopressin-mediated coronary vasospasm, this study was designed to assess effects of vasopressin compared to saline placebo on left anterior descending (lad) coronary artery blood flow. twelve anaesthetized domestic swine were prepared for lad coronary artery blood flow measurement with ultrasonic flow probes, using cardiopulmonary by-pass adjusted to 10% of the prearrest cardiac output. this 10% value approximates that reported for cardiac output during convention ...	2004	15294409
paclitaxel balloon coating, a novel method for prevention and therapy of restenosis.	drug-eluting stents have shown promising antirestenotic effects in clinical trials. non-stent-based local delivery of antiproliferative drugs may offer additional flexibility and also reach vessel areas beyond the immediate stent coverage. the aim of the present study was to evaluate a novel method of local drug delivery based on angioplasty balloons.	2004	15302790
scavenger properties of cultivated pig liver endothelial cells.	background: the liver sinusoidal endothelial cells (lsec) and kupffer cells constitute the most powerful scavenger system in the body. various waste macromolecules, continuously released from tissues in large quantities as a consequence of normal catabolic processes are cleared by the lsec. in spite of the fact that pig livers are used in a wide range of experimental settings, the scavenger properties of pig lsec has not been investigated until now. therefore, we studied the endocytosis and intr ...	2004	15306034
analysis of the successional patterns of insects on carrion in southwest virginia.	studies of carrion-insect succession on domestic pig, sus scrofa l., were conducted in the spring and summer of 2001 and 2002 in blacksburg, va, to identify and analyze the successional patterns of the taxa of forensic importance in southwest virginia. forty-seven insect taxa were collected in the spring. these were represented by 11 families (diptera: calliphoridae, sarcophagidae, muscidae, sepsidae, piophilidae; coleoptera: staphylinidae, silphidae, cleridae, trogidae, dermestidae, histeridae) ...	2004	15311476
characterisation of trichinella isolates from bulgaria by molecular typing and cross-breeding.	trichinella spp. larvae were collected from domestic and wild-life animals in association with 15 human trichinellosis outbreaks registered between 1999-2002 in bulgaria. furthermore, trichinella spp. isolates were obtained from 62 naturally infected wild animals and of a rat. all isolates were subjected to speciation by both multiplex pcr and cross-breeding experiments. epidemiological and clinical data were collected and analysed using standard protocols for epidemiological surveillance and co ...	2004	15325044
two african swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities.	african swine fever virus (asfv), a large icosahedral deoxyvirus, is the causative agent of an economically relevant hemorrhagic disease that affects domestic pigs. the major purpose of the present study was to investigate the nuclear transport activities of the asfv p37 and p14 proteins, which result from the proteolytic processing of a common precursor. experiments were performed by using yeast-based nucleocytoplasmic transport assays and by analysis of the subcellular localization of differen ...	2004	15331706
phylogenetic relationships of australian and new zealand feral pigs assessed by mitochondrial control region sequence and nuclear gpip genotype.	pigs were introduced into australia and new zealand in the 18th and 19th centuries, with some establishing feral populations. with few records of pig introductions into these two countries, molecular phylogenetic analysis was used to assess their origins. mitochondrial (mt) control region sequence and nuclear glucosephosphate isomerase pseudogene (gpip) restriction fragments were used, as distinct european and asian domestic pig and wild boar control region clades and gpip genotypes can be recog ...	2004	15336669
adventitial vasa vasorum heterogeneity among different vascular beds.	different vascular beds show substantial variation in their susceptibilities for development of vascular disease like atherosclerosis, and thereby exhibit a variety of different clinical presentations. yet, the underlying mechanism of this heterogeneity is not well defined. recent evidence suggests a role for the vasa vasorum (vv) in vascular disease. we hypothesized that there is a differential distribution structure of adventitial vv in different vascular beds. hence, the current study was des ...	2004	15337884
laser-assisted end-to-end vascular anastomosis of elastin heterograft to carotid artery with an albumin stent in vivo.	laser-assisted end-to-end vascular anastomosis of an elastin heterograft to native artery may avoid the problems associated with currently available vascular synthetic grafts and conventional suture anastomosis.	2004	15345171
histochemical properties of fibre types in muscles of wild and domestic pigs and the effect of growth rate on muscle fibre properties.	this study reports the results of a comparison of the histochemical properties of various types of porcine muscles in wild and domestic pigs. in domestic pigs, the influence of growth rates on these properties were studied in five muscles: longissimus dorsi (ld), semimembranosus (sm), gluteus superficialis (gs), infra spinam (is) and masseter (mas). the growth rate was expressed as: (i) live weight (lw) at 165±2 days and (ii) daily lw gain from day 88 to day 165 (dg) on the experimental diet. ld ...	2004	22061529
isolation and morphology of single purkinje cells from the porcine heart.	purkinje cells were isolated from both ventricles of young adult domestic pigs and examined by transmitted light or laser scanning confocal microscopy. purkinje cells in free running purkinje fibres were organised in multicellular strands where individual cells were tightly connected end-to-end and closely side-to-side. after isolation, single cells gradually lost the elongated appearance and became more rounded, but the cell membrane remained smooth and undamaged. the contractile material was n ...	2003	14986884
do domestic pigs in controlled environments contrafreeload?	many experiments report that animals will work (lever press) for food in the presence of freely available identical food. this phenomenon has attracted the attention of applied ethologists because it seems to prove that animals have a need to express appetitive behavior. if this is the case, then it has implications for the provision of environmental enrichment for animals in captivity. in this experiment, we maintained 6 pigs in closed economy environments for three 120-hr choice periods in whi ...	2003	14965785
bipolar radiofrequency ablation of the kidney: comparison with monopolar radiofrequency ablation.	we report initial ex vivo and in vivo studies using bipolar radiofrequency (rf) ablation of porcine kidneys. an internal ground electrode is positioned in the kidney opposite the rf electrode, resulting in ablation of all the intervening renal tissue.	2003	14744366
pressure-related hemodynamic effects of co2 pneumoperitoneum in a model of acute cardiac failure.	the adverse effects related to co2 pneumoperitoneum (pp) have been well documented. although these effects in the state of cardiac failure have not been investigated, it appears to be common current clinical practice to use low-pressure pp in this clinical setting, assuming it to be safer.	2003	14733695
a comparison of laparoscopic bipolar vessel sealing devices in the hemostasis of small-, medium-, and large-sized arteries.	the development of new energy sources for hemostasis has facilitated advanced laparoscopic procedures. few studies, however, have documented the strength of the vessels sealed or the extent of surrounding lateral thermal injury, two important factors in maintaining hemostasis while preventing injury to surrounding structures. this study compared the burst pressure and extent of thermal injury of vessels sealed with the 5-mm laparoscopic plasmakinetics trade mark sealer (pk) (gyrus medical, maple ...	2003	14733701
efficacy of ultrasonic tissue dissector and tissue glue for laparoscopic partial nephrectomy in a porcine model.	the purpose of this study was to evaluate the efficacy and long-term tissue effects of ultrasonic dissector and cyanoacrylate glue during laparoscopic partial nephrectomy in a porcine model. nine domestic pigs underwent laparoscopic left lower pole partial nephrectomy without vascular control. an ultrasonic tissue dissector was used to assist in the parenchymal incision, and the raw surfaces of the kidneys, including the exposed urinary system, were sealed with cyanoacrylate glue. no internal st ...	2003	14717525
effects of statins on myocardial and coronary artery response to ischemia-reperfusion.	this study tested the hypotheses that (i) lipophilic statins (atorvastatin and simvastatin) impair ventricular recovery from myocardial ischemia-reperfusion, owing to their greater myocyte permeability, compared with a hydrophilic statin (pravastatin), and (ii) statins enhance endothelium-dependent vasodilation of isolated coronary arteries from the ischemic region. farm pigs consumed chow supplemented with atorvastatin (2.5 mg.kg(-1).d(-1); n=6), pravastatin (10 (n=3) or 20 (n=2) mg.kg(-1).d(-1 ...	2003	14719042
experimental infection of pregnant gilts with swine hepatitis e virus.	to determine the effect of swine hepatitis e virus (hev) infection on pregnant gilts, their fetuses, and offspring, 12 gilts were intravenously inoculated with swine hev. six gilts, who were not inoculated, served as controls. all inoculated gilts became actively infected and shed hev in feces, but vertical transmission was not detected in the fetuses. there was no evidence of clinical disease in the gilts or their offspring. mild multifocal lymphohistiocytic hepatitis was observed in 4 of 12 in ...	2003	14620868
evidence favoring the use of an alpha2-selective vasopressor agent for cardiopulmonary resuscitation.	both alpha1- and beta-adrenergic agonists increase the severity of global myocardial ischemic injury. we hypothesized that combined beta- and alpha1-adrenergic blockade would improve initial resuscitation and postresuscitation myocardial and neurological functions. we further hypothesized that the resulting alpha2-actions of relatively brief duration would favor improved functions compared with the more prolonged effect of nonadrenergic vasopressin.	2003	14623815
brain metabolism during cardiopulmonary resuscitation assessed with microdialysis.	microdialysis is an established tool to analyse tissue biochemistry, but the value of this technique to monitor cardiopulmonary resuscitation (cpr) effects on cerebral metabolism is unknown. the purpose of this study was to assess the effects of active-compression-decompression (acd) cpr in combination with an inspiratory threshold valve (itv) (=experimental cpr) vs. standard cpr on cerebral metabolism measured with microdialysis.	2003	14625117
immediate defibrillation versus interventions first in a swine model of prolonged ventricular fibrillation.	we compared time-dependent rescue shock success when delivered immediately, to defibrillation preceded by 3 min of cpr, with and without high dose epinephrine (hde) in a swine model of prolonged ventricular fibrillation (vf). our hypotheses were that pretreatment with cpr and hde would produce higher rates of successful first-shock defibrillation and would prevent decay of the vf waveform, as measured by the scaling exponent (sce), when compared to immediate defibrillation. we also sought to det ...	2003	14625118
epidemiology of mycobacterium bovis infections of pigs and wild boars using a molecular approach.	a molecular epidemiological approach was applied to establishing a possible role for the wild boar as a natural reservoir of mycobacterium bovis in sierra de villuercas, western spain; an area free of farmed cattle and wild deer populations. spoligo and vntr typing were used over a three year period to study the epidemiological relationship between the occurrence of bovine tuberculosis (tb) in extensively bred iberian pigs and indigenous wild boar. the 37 sampled wild boar showed different degre ...	2003	14637044
gene structure of pig sterol 12alpha-hydroxylase (cyp8b1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6alpha-hydroxylase (cyp4a21).	cholic acid is the major trihydroxy bile acid formed in most mammals. the domestic pig (sus scrofa) is an exception. the bile of adult pig is devoid of cholic acid whereas hyocholic acid is found in amounts equal to that of cholic acid in humans. the pathway leading to formation of hyocholic acid is believed to be species-specific and to have evolved in the pig to compensate for a nonexistent or deficient cholic acid biosynthesis. however, a high level of cholic acid has recently been found in t ...	2003	14643796
[case report: porcine circovirus type 2 infection in an european wild boar (sus scrofa) in the state of brandenburg, germany].	this case represents the first case of porcine circovirus type 2 (pcv-2)--infection in a free living european wild boar associated with morphological lesions, which are regarded as characteristic for postweaning multisystemic wasting syndrome (pmws) in domestic pigs. the animal, an approximately 10 month old male, was found dead in a rural area within the state of brandenburg, germany. the closest commercial pig farm is located in 3 km distance from the spot where the carcass was found. at necro ...	2003	14650740
mosquito host-feeding patterns and implications for japanese encephalitis virus transmission in northern australia and papua new guinea.	japanese encephalitis (je) virus spread to northern australia during the 1990s, transmitted by culex annulirostris skuse and other mosquitoes (diptera: culicidae). to determine the relative importance of various hosts for potential vectors of je virus, we investigated the host-feeding patterns of mosquitoes in northern australia and western province of papua new guinea, with particular attention to pigs, sus scrofa l. - the main amplifying host of je virus in south-east asia. mosquitoes were col ...	2003	14651654
ropivacaine combined with various anti-arrhythmic drugs results in mild alterations in myocardial contractility in pigs.	the present study was undertaken following the observation of a marked decrease in myocardial contractility after ropivacaine in a patient on amiodarone, in order to investigate the cardiovascular effects of combining ropivacaine with anti-arrhythmic drugs (aard).	2003	14656782
atorvastatin stent coating does not reduce neointimal proliferation after coronary stenting.	statins seem to be suitable for restenosis prevention via reduction of smooth muscle cell proliferation, anti-inflammatory effects, and improvement of endothelial function. the aim of the present study was to test the efficacy of an atorvastatin stent coating for restenosis inhibition in the porcine coronary stent model.	2003	14663613
laparoscopic heminephrectomy using a new fibrin sealant powder.	to determine the ability of a hemostatic fibrin sealant powder (hfsp), delivered laparoscopically, to achieve hemostasis and seal the collecting system during laparoscopic heminephrectomy.	2003	14665378
wild boars (sus scrofa) as reservoirs of brucella suis biovar 2 in croatia.	this work presents the results of findings for brucellosis in wild boars and domestic swine in two regions of croatia. in the region of djakovo the blood samples of 211 wild boars were analysed and in 29.4% of the samples serologically positive reactions were established. in the same region the blood samples of 1080 domestic swine on pastures were also analysed and positive serological reactions were established in 12.3%. in the regions around lonjsko polje the blood samples of 53 wild boars wer ...	2003	14680058
chest wall disruption with and without acute lung injury: effects of continuous positive airway pressure therapy on ventilation and perfusion relationships.	we investigated the evolution of lung injury in an animal model with multiple rib fractures, both with and without acute lung injury, and the influence of spontaneous breathing with continuous positive airway pressure (cpap) therapy on the relative distributions of alveolar ventilation ([ov0312]a) and perfusion ([ov0422]).	2003	14501968
intake of nutrients from pasture by pigs.	the domestic pig evolved from the omnivorous wild boar and has retained many natural foraging behaviour patterns. although most modem commercial pigs are housed and fed concentrate diets, interest in the potential nutrient contribution from pasture has increased due to growth in outdoor and organic pig production systems. the energy requirements of outdoor pigs are increased by approximately 15% under northern european conditions as a result of greater climatic energy demand and locomotory activ ...	2003	14506873
basic fibroblast and epidermal growth factors stimulate survival in adult porcine photoreceptor cell cultures.	to investigate the effects of basic fibroblast and epidermal growth factor (fgf2 and egf, respectively) on the survival and phenotypic expression of photoreceptors isolated from adult mammalian retinas.	2003	14507904
mitochondrial dna sequence variation and phylogenetic relationships among iberian pigs and other domestic and wild pig populations.	nucleotide sequences of mitochondrial dna (mtdna) cytochrome b gene (1140 bp) and control region (707 bp) were used to determine the phylogenetic relationships among 51 pig samples representing ancient and current varieties of iberian pigs (26), spanish wild boars (seven) and other domestic pigs (18) of cosmopolitan (duroc, large white, landrace, pietrain and meishan) and local (spotted black jabugo, basque and mangalitza) breeds. a neighbour-joining tree constructed from pairwise distances prov ...	2003	14510666
experimental model of continent colostomy using rectus abdominis neosphincteroplasty.	use of dynamic myoplasty to create a continent stoma has produced promising results, but long-term stoma continence has not been achieved. the aim of the study was to establish and test a new model.	2003	14515299
genetic typing of recent classical swine fever virus isolates from croatia.	during a period of 5 years (1997-2001) several outbreaks of classical swine fever (csf) were recorded in croatia. for genetic typing, fragments of 150 nucleotides within the 5'-non-translated region (5'-ntr) and 190 nucleotides within the e2 glycoprotein coding gene of nine field isolates that were derived from domestic pigs and wild boars were used. for better epizootiological understanding, isolates from other european countries were included in the study. the results show that the isolates be ...	2003	14516705
increasing esophageal length: a comparison of laparoscopic versus transthoracic esophageal mobilization with and without vagal trunk division in pigs.	new laparoscopic techniques allow both mediastinal mobilization and performance of a collis gastroplasty when necessary, and the utility of a transthoracic approach is questioned. the aim of this study was to compare the increase in esophageal length achievable with laparoscopic and transthoracic esophageal mobilization in pigs, and to assess the impact of vagal trunk division on esophageal length.	2003	14522324
role of birds in transmission of classical swine fever virus.	active transmission of classical swine fever virus (csfv) was studied in six birds (five ravens, one hooded crow) and two laying hens. cloacal swabs, blood and organs of birds and hens as well as blood and organ samples of pigs which had been fed with faeces derived from csfv infected birds or which had come in contact with faeces of infected hens were negative for csfv. none of the animals seroconverted during the study. this result demonstrates that active virus transmission by these animals i ...	2003	14535936
genotyping of clostridium perfringens isolated from domestic and exotic ruminants and swine.	clostridium perfringens types a, b, c, d and e are known to cause severe enteritis/enterotoxaemia and diseases (especially caused by type a) belonging to the gas oedema complex in many species. samples from the small intestine as well as faeces of domestic and exotic animals suffering from enterotoxaemic signs or having died within days after first occurance of toxaemia were submitted for typing c. perfringens toxovars by multiplex pcr. the following species have been investigated: domestic shee ...	2003	14535937
influence of radiographic contrast media on myocardial tissue oxygen tension: nacl-controlled, randomised, comparative study of iohexol versus iopromide in an animal model.	it was tested whether a bolus injection of 10 ml radiographic contrast medium (iopromide vs. iohexol), compared to a 10 ml nacl bolus and administered into the left anterior descending artery (lad) of farm pigs, influenced the tissue po2 in the territory of this artery. the radiographic contrast media and the nacl bolus were given in randomised order. the mean po2 lad fell from initially 40.3+/-10.9 mmhg to a minimal value of 22.5+/-8.9 mmhg 241+/-44 sec after injecting the iopromide bolus, with ...	2003	14561904
spinal blood flow in 24-hour megadose glucocorticoid treatment in awake pigs.	because of the controversy regarding the benefits of 24-hour administration of methylprednisolone in patients with spinal cord injury (sci), it is important to investigate its mechanism of action and side effects. this study was conducted to determine if high-dose methylprednisolone modulates neural and vertebral blood flow in an awake large-sized animal model without sci.	2003	14563146
potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine.	free-ranging feral swine (sus scrofa) are known to be present in at least 32 states of the usa and are continuously expanding their range. infection with pseudorabies virus (prv) occurs in feral swine and the primary route of transmission in free-living conditions seems to be venereal. between 1995 and 1999, naturally infected feral swine and experimentally infected hybrid progeny of feral and domestic swine, were kept in isolation and evaluated for occurrence of latent prv indigenous to feral s ...	2003	14567217
111in oxine labelled mesenchymal stem cell spect after intravenous administration in myocardial infarction.	mesenchymal stem cells (mscs) have shown therapeutic potential if successfully delivered to the intended site of myocardial infarction. the purpose of this pilot study was to test the feasibility of 111in oxine labelling of mscs and single photon emission computed tomography (spect) imaging after intravenous administration in a porcine model of myocardial infarction. adult farm pigs (n=2) were subjected to closed chest experimental myocardial infarction. 111in oxine labelled mscs (1 x 10(7) to 2 ...	2003	14569169
effects of the endothelin-1 receptor antagonist tezosentan on renal blood flow and diuresis during prolonged increased intra-abdominal pressure.	it has earlier been shown that increased intra-abdominal pressure (iap) reduces renal blood circulation and urine output both clinically and experimentally. the aim of this study was to investigate the effect of endothelin-1 inhibition by the endothelin-1 receptor antagonist tezosentan on renal blood circulation and diuresis in pigs subjected to prolonged increased intra-abdominal pressure.	2003	14572770
periocular injection of microspheres containing pkc412 inhibits choroidal neovascularization in a porcine model.	oral administration of pkc412, a kinase inhibitor that blocks several isoforms of protein kinase c (pkc) and receptors for vascular endothelial growth factor (vegf), platelet-derived growth factor, and stem cell factor, inhibits ocular neovascularization in a murine model. the purpose of this study was to determine whether sustained local delivery of pkc412 in a human-sized eye inhibits choroidal neovascularization (cnv).	2003	14578426
should we ventilate or cool the pulmonary graft inside the non-heart-beating donor?	the ideal preservation method during the warm ischemic period in the non-heart-beating donor (nhbd) remains unclear. in this study we compare the protective effect of ventilation vs cooling of the non-perfused pulmonary graft.	2003	14585384
interactions between tsetse and trypanosomes with implications for the control of trypanosomiasis.	tsetse flies (diptera: glossinidae) are vectors of several species of pathogenic trypanosomes in tropical africa. human african trypanosomiasis (hat) is a zoonosis caused by trypanosoma brucei rhodesiense in east africa and t. b. gambiense in west and central africa. about 100000 new cases are reported per year, with many more probably remaining undetected. sixty million people living in 36 countries are at risk of infection. recently, t. b. gambiense trypanosomiasis has emerged as a major publi ...	2003	14587696
the postnatal growth of the beta-cell mass in pigs.	studies of the postnatal growth of the beta-cell mass in rats have revealed some unexpected and apparently paradoxical results, the most prominent being a beta-cell mass plateau in the early phase of life. we have studied the postnatal growth of the beta-cell mass in the domestic pig to investigate its development in a larger mammal. the pancreases from a total of 86 male pigs from 5 to 100 days of age were studied. the beta-cell mass increased linearly from day 5 to day 40, reached a plateau fr ...	2003	14596676
vaccinology of classical swine fever: from lab to field.	there are two types of classical swine fever vaccines available: the classical live and the recently developed e2 subunit vaccines. the live chinese strain vaccine is the most widely used. after a single vaccination, it confers solid immunity within a few days that appears to persist lifelong. the e2 subunit vaccine induces immunity from approximately 10-14 days after a single vaccination. the immunity may persist for more than a year, but is then not complete. the chinese strain vaccine may est ...	2003	14599784
an investigation into the wound-healing properties of essential oil of ocimum gratissimum linn.	to investigate the effects of ocimum oil and two antibacterial preparations, cicatrin (glaxowellcome) and cetavlex (astrazeneca), on the healing of full-thickness excisional and incisional wounds, created under anaesthesia, on the back of test and control groups of adult albino rabbits.	2003	14601223
evaluation of a vessel sealing system, bipolar electrosurgery, harmonic scalpel, titanium clips, endoscopic gastrointestinal anastomosis vascular staples and sutures for arterial and venous ligation in a porcine model.	we assessed the usefulness of the ligasure (valleylab, boulder, colorado) vessel sealing system for vascular control during laparoscopic surgery and compared it with other available hemostatic modalities.	2003	12544345
pathogenicity and kinetics of virus propagation in swine infected with the cytopathogenic classical swine fever virus containing defective interfering particles.	to analyze the pathogenicity and in vivo kinetics of the cytopathogenic (cp) classical swine fever virus (csfv) wb82 strain, which is composed of cp defective interfering (di) particles and noncytopathogenic (noncp) helper virus (wb82/e(+) strain), wb82 and wb82/e(+) strains were administered separately to domestic pigs. after inoculation with either strain, all pigs showed typical symptoms of classical swine fever (csf), such as leucopenia and high fever. there were few differences in clinical ...	2003	12556994
the effect of birth weight on hypothalamo-pituitary-adrenal axis function in juvenile and adult pigs.	programming of the hypothalamo-pituitary-adrenal (hpa) axis during prenatal and early postnatal life may explain, in part, the association between low birth weight (bw) and the increased incidence of cardiovascular and metabolic disease in later life. this study examined the effect of natural variations in bw on hpa axis function in juvenile and adult pigs. low (< 1.47 kg) and high (> 1.53 kg) bw pure-bred large white piglets from 15 litters were studied at 3 (n = 47) and 12 (n = 17) months of a ...	2003	12562947
bacteriological and genetic assessment of game meat from japanese wild boars.	bacterial tests were used to assess bacterial contamination of game meat from japanese wild boars. the bacterial contamination of wild boar meat was less than that of domestic pork, as determined by aerobic plate counts (apc) and coliform counts. none of the meat examined in this study was contaminated by salmonella or e. coli o-157. to detect adulteration by domestic pig meat or european wild boar meat, 46 samples of game meat sold as japanese wild boar were examined genetically. a total of 17 ...	2003	12517093
classical swine fever--an update.	classical swine fever (csf) is a serious and contagious viral disease of pigs and wild boar with a widespread worldwide distribution. the immunopathology of the disease is poorly understood, but the ability of the csf virus to infect cells without triggering apoptosis and to kill uninfected cells is probably highly significant. the virus may be spread by various direct and indirect methods, but in most cases the exact mechanisms involved in local spread between farms are not known. excellent dia ...	2003	13129664
[studies on the karyotype and bands of hybrid pig].	using usual method, we got karyotype of hybrid pig (wild soar (male symbol) x domestic pig (female symbol)), c-band and approximate 174 bands of g-band, and we also obtained approximate 258 bands of high resolution g-band by micro-colchicin method. the result indicate that the diploid chromosome number is 2n=38; there is polymorphism in c-band,and compared with domestic pig in g-band and high resolution g-band there is no distinguish difference. they belong to the same seed.	2003	15639955
ginkgo biloba extract egb 761 attenuates myocardial stunning in the pig heart.	myocardial stunning, a transient contractile dysfunction that appears following a brief period of ischemia, is at least partly due to the production of oxygen-derived free radicals. the objective of the present study was to determine whether the ginkgo biloba extract egb761, which has antioxidant properties in vitro, can attenuate myocardial stunning in vivo. forty-seven anesthetized open-chest farm pigs underwent 10 min of occlusion of the left anterior descending coronary artery (lad), followe ...	2003	12494270
transluminal stent graft repair with wallgraft endoprosthesis in a porcine arteriovenous graft pseudoaneurysm model.	pseudoaneurysm is a known complication of arteriovenous grafts in chronic hemodialysis and can result in graft disruption or thrombosis if left untreated. this study evaluated the safety and efficacy of endovascular repair with wallgraft endoprosthesis (boston scientific, inc, watertown, mass) in a porcine arteriovenous graft (avg) pseudoaneurysm model. materials and methods: bilateral groin avg pseudoaneurysms (n = 18) were created with an oversized dacron interposition graft within a polytetra ...	2003	12514597
magnetic resonance image-guided transcatheter closure of atrial septal defects.	recent developments in cardiac mri have extended the potential spectrum of diagnostic and interventional applications. the purpose of this study was to test the ability of mri to perform transcatheter closures of secundum type atrial septal defects (asd) and to assess asd size and changes in right cardiac chamber volumes in the same investigation.	2003	12515755
direct intrahepatic portacaval shunt: an experimental study.	to determine the feasibility of creating direct intrahepatic portacaval shunt (dips) in swine with puncture under sonographic guidance.	2003	12532459
liver sinusoidal endothelial cells represents an important blood clearance system in pigs.	background: numerous studies in rats and a few other mammalian species, including man, have shown that the sinusoidal cells constitute an important part of liver function. in the pig, however, which is frequently used in studies on liver transplantation and liver failure models, our knowledge about the function of hepatic sinusoidal cells is scarce. we have explored the scavenger function of pig liver sinusoidal endothelial cells (lsec), a cell type that in other mammals performs vital eliminati ...	2003	12537582
nipah virus outbreak in malaysia.	nipah virus, a novel paramyxovirus, closely related to hendra virus emerged in northern part of peninsular malaysia in 1998. the virus caused an outbreak of severe febrile encephalitis in humans with a high mortality rate, whereas, in pigs, encephalitis and respiratory diseases but with a relatively low mortality rate. the outbreak subsequently spread to various regions of the country and singapore in the south due to the movement of infected pigs. nipah virus caused systemic infections in human ...	2003	12637075
creation of ureteropelvic junction obstruction and its correction by chemical glue-assisted laparoscopic dismembered pyeloplasty.	we established a porcine model of ureteropelvic junction (upj) obstruction using a laparoscopic technique and assessed the outcome of standard suture-assisted and chemical glue-assisted laparoscopic pyeloplasty.	2003	12639357
effects of cryoablation on short-term development of urinary fistulas in the porcine kidney.	to determine the effect of cryoablation on the collecting system in the porcine kidney and the possible development of pelvic injury or fistula.	2003	12639360
in vivo validation of cardiac spiral computed tomography using retrospective gating.	cardiac functional assessment represents the basis for diagnostics and cardiac operation planning. spiral computed tomography (ct) combines the advantages of three-dimensional imaging and high temporal resolution when using gating techniques. however, in vivo validation data of this novel imaging technology are lacking. the purpose of this study was to validate in vivo the new imaging method using retrospective gating and to evaluate the clinical usefulness of the achieved temporal resolution.	2003	12645712
1-molar gadobutrol as a contrast agent for computed tomography: results from a comparative porcine study.	to evaluate prospectively the efficacy of gadobutrol as contrast agent for computed tomography (ct) compared with iodinated contrast media in a porcine animal model.	2003	12649642
studies of the transmissibility of the agent of bovine spongiform encephalopathy to pigs.	studies to test the transmissibility of the bovine spongiform encephalopathy (bse) agent to pigs began in 1989. parenteral inoculation of the agent by three routes simultaneously (intracranially, intravenously and intraperitoneally) produced disease with an incubation period range of 69-150 weeks. pre-clinical pathological changes were detected in two pigs killed electively at 105 and 106 weeks post-inoculation. infectivity was detected by bioassay in inbred mice in the cns of those pigs that de ...	2003	12655106
histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography.	to compare ultrahigh-resolution optical coherence tomography (oct) cross-sectional images of the pig retina with histology, to evaluate the potential of ultrahigh-resolution oct for enhanced visualization of intra- and subretinal structures.	2003	12657611
anatomical and neurochemical features of the extrinsic and intrinsic innervation of the striated muscle in the porcine esophagus: evidence for regional and species differences.	studies of the intrinsic and extrinsic innervation patterns of esophageal motor endplates (meps) are mainly confined to small rodents. therefore, an immunocytochemical, denervation and tracing study was conducted on the pig, an experimental model in which the distribution of the striated esophageal muscle portion more closely resembles the human situation. the purpose of this study was to analyze the origin and neurochemical content of the nerve fibers participating in the myoneural synapse. fif ...	2003	12658437
segmental nature of the porcine liver and its potential as a model for experimental partial hepatectomy.	in-depth knowledge of pig liver anatomy allows potential research into segmental liver resections and hepatic regeneration, as well as liver transplantation techniques. the segmental anatomy, however, remains largely unknown. this study aimed to delineate the segmental anatomy of the porcine liver in comparison with that of the human.	2003	12673745
effect of post-mortem changes on peripheral and central whole blood and tissue clozapine and norclozapine concentrations in the domestic pig (sus scrofa).	interpretation of the results of psychoactive or other drug measurements in post-mortem blood specimens may not be straightforward, in part because analyte concentrations in blood may change after death. there is also the issue of comparability of plasma (or serum) results to those obtained in whole blood. to investigate these problems with respect to clozapine, this drug (10mg/kg daily) was given orally to two pigs. blood was collected 3h post-dose on day 7, the animals were sacrificed, and blo ...	2003	12689746
characterization of japanese swine and human hepatitis e virus isolates of genotype iv with 99 % identity over the entire genome.	the full-length genomic sequences were determined of japanese swine and human hepatitis e virus (hev) isolates (swj13-1 and he-ja1, respectively) with 100 % identity in the partial sequence of open reading frame (orf) 2 (orf2, 412 nt). swj13-1 was isolated from a 4-month-old farm pig born in hokkaido, japan, in 2002 and he-ja1 was recovered from a 55-year-old patient who lived in hokkaido and who had contracted sporadic acute hepatitis e in 1997. both isolates consisted of 7240 nt, excluding the ...	2003	12692290
large hepatic ablation with bipolar saline-enhanced radiofrequency: an experimental study in in vivo porcine liver with a novel approach.	radiofrequency ablation (rfa) is a relatively new technology for the local destruction of liver tumors. development of recent devices has enabled the creation of larger lesions. nevertheless, treating liver tumors larger than 2.5 cm in diameter often requires multiple overlapping ablations to encompass the tumor and the surrounding healthy tissue rim with an increasing risk of local recurrence.	2003	12697400
a novel porcine gammaherpesvirus.	a novel porcine gammaherpesvirus was detected in the blood of domestic pigs by pcr. with degenerate-primer pcr and subsequent long-distance pcr approaches a 60-kbp genome stretch was amplified. sequence analysis revealed the presence of the gammaherpesvirus orfs 03 to 46 as well as a putative chemokine receptor and a v-bcl-2 gene. the 60-kbp sequence was compared with the corresponding sequence of the porcine lymphotropic herpesvirus 1 (plhv-1) published recently and the sequence of plhv-2, whic ...	2003	12706081
alterations in bronchoalveolar lavage fluid during ischemia-induced acute hepatic failure in the pig.	the objective of this controlled experimental animal study was to evaluate whether acute hepatic failure (ahf) can cause acute lung injury (ali) and to investigate possible pathophysiologic mechanisms. seventeen domestic pigs were randomly assigned to ahf and sham groups. ahf was induced by surgical devascularization of liver in 10 animals. seven animals were sham operated. hemodynamics, lung mechanics, extravascular lung water (evlw), and intracranial pressure, blood gas, liver function tests, ...	2003	12717394
initial experiences with in vivo intravascular coronary vessel wall imaging.	to evaluate potential use of a loopless internal receiver coil for in vivo coronary artery vessel wall imaging in five domestic swine.	2003	12720273
[an acute hepatitis case domestically infected with a hepatitis e virus whose nucleotide sequence showed a high similarity to that from a domestic swine in japan].		2003	12722353
classical swine fever in wild boar in the lombardy region of italy from 1997 to 2002.	in 1997, outbreaks of classical swine fever occurred in wild boar and domestic pigs in the italian province of varese (lombardy region). statutory animal health measures were imposed, and a control plan aimed at preventing the virus from spreading outside the infected hunting zone of 108 km2 was implemented. however, in 1998, virologically positive wild boar were found outside this zone, including within swiss territory. the infected zone was then enlarged to include the three other hunting area ...	2003	12723629
in vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction.	we investigated the potential of magnetic resonance imaging (mri) to track magnetically labeled mesenchymal stem cells (mr-mscs) in a swine myocardial infarction (mi) model.	2003	12732608
robotically assisted laparoscopic dismembered pyeloplasty: a chronic porcine study.	to evaluate the feasibility and efficacy of robotically assisted laparoscopic dismembered pyeloplasty in an animal model. we also investigated whether the use of a device that lacks tactile feedback results in tissue damage and subsequent stenosis or leaking of the anastomosis.	2003	12736048
effect of combining nicotinamide as a pars-inhibitor with selective inos blockade during porcine endotoxemia.	to investigate the effects of combined selective inducible nitric oxide synthase (inos) inhibition using 1400 w with nicotinamide (nad) as a pars-inhibitor on hepato-splanchnic hemodynamics, o(2) kinetics, and energy metabolism during hyperdynamic porcine endotoxemia.	2003	12739012
intramural delivery of recombinant apolipoprotein a-imilano/phospholipid complex (etc-216) inhibits in-stent stenosis in porcine coronary arteries.	we have previously demonstrated vasculoprotective effects after repeated intravenous administration of recombinant apolipoprotein a-imilano (apoa-im)/phospholipid complex. in this study, we sought to determine the effects of local recombinant apoa-im/1-palmitoyl,2-oleoyl phosphatidylcholine complex (etc-216) delivered intramurally via the infiltrator catheter on luminal narrowing in a porcine coronary artery stent overstretch injury model.	2003	12742990