Publications

de novo adult transcriptomes of two european brittle stars: spotlight on opsin-based photoreception.	next generation sequencing (ngs) technology allows to obtain a deeper and more complete view of transcriptomes. for non-model or emerging model marine organisms, ngs technologies offer a great opportunity for rapid access to genetic information. in this study, paired-end illumina hiseqtm technology has been employed to analyse transcriptomes from the arm tissues of two european brittle star species, amphiura filiformis and ophiopsila aranea. about 48 million illumina reads were generated and 136 ...	2016	27119739
excretion of dengue virus rna by aedes aegypti allows non-destructive monitoring of viral dissemination in individual mosquitoes.	successful transmission of a vector-borne pathogen relies on a complex life cycle in the arthropod vector that requires initial infection of the digestive tract followed by systemic viral dissemination. the time interval between acquisition and subsequent transmission of the pathogen, called the extrinsic incubation period, is one of the most influential parameters of vector-borne pathogen transmission. however, the dynamic nature of this process is often ignored because vector competence assays ...	2016	27117953
droplet characterization and penetration of an ultra-low volume mosquito adulticide spray targeting the asian tiger mosquito, aedes albopictus, within urban and suburban environments of northeastern usa.	adult control of aedes albopictus via ultra-low volume is difficult because this species occurs primarily in peridomestic habitats where obstacles such as buildings and vegetation can disrupt spray plumes and droplet dispersion. we determined droplet penetration and characterization of a pyrethroid adulticide applied from the ground at mid (46.77 ml/ha) and maximum (93.53 ml/ha) label rates within cryptic habitats of urban and suburban environments. droplets were collected from all habitats, wit ...	2016	27116103
knowledge, attitudes, and practices of florida physicians regarding dengue before and after an educational intervention.	failure to recognize and appropriately manage dengue early in the clinical course may result in late initiation of supportive treatment for severe disease. in florida, travel-related and autochthonous dengue occur and are likely under-recognized. the objective of this study was to evaluate physician knowledge of dengue and its management before and after an educational intervention in florida.	2016	27112138
pfsmad4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster pinctada fucata.	mollusca is the second largest phylum in nature. the shell of molluscs is a remarkable example of a natural composite biomaterial. biomineralization and how it affects mollusks is a popular research topic. the bmp-2 signaling pathway plays a canonical role in biomineralization. smad4 is an intracellular transmitter in the bmp signaling pathway in mammals, and some genomic data show smad4's involvement in bmp signaling in invertebrates, but whether smad4 plays a conservative role in pearl oyster, ...	2016	27113217
clinical profile of dengue fever in children: a study from southern odisha, india.	background. in india, dengue epidemics are becoming more frequent (who, 2008). the majority of dengue viral infections are self-limiting, but complications may cause high morbidity and mortality. objectives. to assess the clinical profile of the dengue infection in children less than 14 years of age and to evaluate the outcomes of dengue fever from september 2013 to august 2015 at the pediatric department of maharaja krishna chandra gajapati medical college, the largest tertiary care hospital of ...	2016	27213083
inferring the spatio-temporal patterns of dengue transmission from surveillance data in guangzhou, china.	dengue is a serious vector-borne disease, and incidence rates have significantly increased during the past few years, particularly in 2014 in guangzhou. the current situation is more complicated, due to various factors such as climate warming, urbanization, population increase, and human mobility. the purpose of this study is to detect dengue transmission patterns and identify the disease dispersion dynamics in guangzhou, china.	2016	27105350
sequence capture by hybridization to explore modern and ancient genomic diversity in model and nonmodel organisms.	the recent expansion of next-generation sequencing has significantly improved biological research. nevertheless, deep exploration of genomes or metagenomic samples remains difficult because of the sequencing depth and the associated costs required. therefore, different partitioning strategies have been developed to sequence informative subsets of studied genomes. among these strategies, hybridization capture has proven to be an innovative and efficient tool for targeting and enriching specific b ...	2016	27105841
enhancement of aedes albopictus collections by ovitrap and sticky adult trap.	in the last decades, aedes albopictus has become an increasing public health threat in tropical as well as in more recently invaded temperate areas due to its capacity to transmit several human arboviruses, among which dengue, chikungunya and zika. enhancing the efficiency of currently used collection approaches, such as ovitraps and sticky traps, is desirable for optimal monitoring of the species abundance, for assessment of the risk of arbovirus transmission and for the optimisation of control ...	2016	27102015
in vitro template-change pcr to create single crossover libraries: a case study with b. thuringiensis cry2a toxins.	during evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequence variation and function. however, no well-developed in vitro method has been established to create single-crossover libraries. here we present an in vitro template-change polymerase change reaction ...	2016	27097519
association between nutritional status and dengue infection: a systematic review and meta-analysis.	dengue infection has various clinical manifestations, often with unpredictable clinical evolutions and outcomes. several factors including nutritional status have been studied to find the relationship with dengue severity. however, the nutritional status had conflicting effects on the complication of dengue in some previous studies. therefore, we conducted a systematic review and performed a meta-analysis to analyze the association between nutritional status and the outcome of dengue infection.	2016	27097934
testing the causality between cyp9m10 and pyrethroid resistance using the talen and crispr/cas9 technologies.	recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. for studies on insecticide resistance, genome editing technologies offer some advantages over the conventional reverse genetic technique, rna interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. there were relatively abundant evidences indicating that the overexpression of a cytochrome p450 gene cyp9m10 confers s ...	2016	27095599
international air travel to ohio, usa, and the impact on malaria, influenza, and hepatitis a.	the state of ohio led the united states in measles in 2014, ostensibly related to international air travel (iat), and ranked lower than 43 other states in infectious disease outbreak preparedness. we conducted a retrospective cohort study using surveillance data of the total ohio population of 11 million from 2010 through 2014 with a nested case control of air travelers to determine the risk of malaria, seasonal influenza hospitalizations (ih), and hepatitis a (ha) disease related to internation ...	2016	27123365
prediction of dengue outbreaks based on disease surveillance and meteorological data.	research is needed to create early warnings of dengue outbreaks to inform stakeholders and control the disease. this analysis composes of a comparative set of prediction models including only meteorological variables; only lag variables of disease surveillance; as well as combinations of meteorological and lag disease surveillance variables. generalized linear regression models were used to fit relationships between the predictor variables and the dengue surveillance data as outcome variable on ...	2016	27031524
evocation to the dr. carlos j. finlay barres on the centennial of his death.	about the editorial of the professor guillermo llanos: "carlos j. finlay: the forgotten pasteur of america", a hundred years after his death and through a documental review, a summary of the life and work of this great man of science was conducted. finlay was a notable figure of the american medicine, he conceived a new infection way able to explain the propagation of the yellow fever, and added the possibility of their scientific confirmation by an experimental method. for all the above-mention ...	2016	27226667
tor pathway-mediated juvenile hormone synthesis regulates nutrient-dependent female reproduction in nilaparvata lugens (stål).	the "target of rapamycin" (tor) nutritional signaling pathway and juvenile hormone (jh) regulation of vitellogenesis has been known for a long time. however, the interplay between these two pathways regulating vitellogenin (vg) expression remains obscure. here, we first demonstrated the key role of amino acids (aas) in activation of vg synthesis and egg development in nilaparvata lugens using chemically defined artificial diets. aas induced the expression of tor and s6k (s6 kinase), whereas rnai ...	2016	27043527
virtual screening of plant volatile compounds reveals a high affinity of hylamorpha elegans (coleoptera: scarabaeidae) odorant-binding proteins for sesquiterpenes from its native host.	hylamorpha elegans(burmeister) is a native chilean scarab beetle considered to be a relevant agricultural pest to pasture and cereal and small fruit crops. because of their cryptic habits, control with conventional methods is difficult; therefore, alternative and environmentally friendly control strategies are highly desirable. the study of proteins that participate in the recognition of odorants, such as odorant-binding proteins (obps), offers interesting opportunities to identify new compounds ...	2016	27012867
identification and characterization of two "sensory neuron membrane proteins" (snmps) of the desert locust, schistocerca gregaria (orthoptera: acrididae).	pheromone-responsive neurons of insects not only require specific receptors but in addition several auxiliary components, including the "sensory neuron membrane protein," snmp. accordingly, snmp is considered as a marker for neurons responding to pheromones. for the desert locust schistocerca gregaria, it is known that the behavior, including aggregation behavior and courtship inhibition, is largely controlled by pheromones. however, little is known about pheromones, their receptors, and the phe ...	2016	27012870
correction: how diverse detrital environments influence nutrient stoichiometry between males and females of the co-occurring container mosquitoes aedes albopictus, ae. aegypti, and culex quinquefasciatus.		2016	27007775
genome-wide analysis of ionotropic receptors provides insight into their evolution in heliconius butterflies.	in a world of chemical cues, smell and taste are essential senses for survival. here we focused on heliconius, a diverse group of butterflies that exhibit variation in pre- and post-zygotic isolation and chemically-mediated behaviors across their phylogeny. our study examined the ionotropic receptors, a recently discovered class of receptors that are some of the most ancient chemical receptors.	2016	27004525
risk associated with the release of wolbachia-infected aedes aegypti mosquitoes into the environment in an effort to control dengue.	in an effort to eliminate dengue, a successful technology was developed with the stable introduction of the obligate intracellular bacteria wolbachia pipientis into the mosquito aedes aegypti to reduce its ability to transmit dengue fever due to life shortening and inhibition of viral replication effects. an analysis of risk was required before considering release of the modified mosquito into the environment.	2016	27047911
minocycline as a re-purposed anti-wolbachia macrofilaricide: superiority compared with doxycycline regimens in a murine infection model of human lymphatic filariasis.	lymphatic filariasis and onchocerciasis are parasitic helminth diseases, which cause severe morbidities such as elephantiasis, skin disease and blindness, presenting a major public health burden in endemic communities. the anti-wolbachia consortium (a·wol: http://www.a-wol.com/) has identified a number of registered antibiotics that target the endosymbiotic bacterium, wolbachia, delivering macrofilaricidal activity. here we use pharmacokinetics/pharmacodynamics (pk/pd) analysis to rationally dev ...	2016	26996237
is dengue vector control deficient in effectiveness or evidence?: systematic review and meta-analysis.	although a vaccine could be available as early as 2016, vector control remains the primary approach used to prevent dengue, the most common and widespread arbovirus of humans worldwide. we reviewed the evidence for effectiveness of vector control methods in reducing its transmission.	2016	26986468
spectrum of maternofetal outcomes during dengue infection in pregnancy: an insight.	dengue is a vector transmitted viral infection; tropical and subtropical countries see outbreaks of dengue each year. there is a paucity of literature on effects of dengue infection on pregnancy outcome and this prompted us to undertake a study for better understanding of pregnancy implications with dengue infection. pregnant women admitted during the seasonal outbreak of dengue between september 2015 and october 2015 were studied and maternal and fetal outcomes in sixteen ns1ag positive women w ...	2016	27069349
the hitchhiker's guide to europe: the infection dynamics of an ongoing wolbachia invasion and mitochondrial selective sweep in rhagoletis cerasi.	wolbachia is a maternally inherited and ubiquitous endosymbiont of insects. it can hijack host reproduction by manipulations such as cytoplasmic incompatibility (ci) to enhance vertical transmission. horizontal transmission of wolbachia can also result in the colonization of new mitochondrial lineages. in this study, we present a 15-year-long survey of wolbachia in the cherry fruit fly rhagoletis cerasi across europe and the spatiotemporal distribution of two prevalent strains, wcer1 and wcer2, ...	2016	26846713
sfdredd, a novel initiator caspase possessing activity on effector caspase substrates in spodoptera frugiperda.	sf9, a cell line derived from spodoptera frugiperda, is an ideal model organism for studying insect apoptosis. the first notable study that attempted to identify the apoptotic pathway in sf9 was performed in 1997 and included the discovery of sf-caspase-1, an effector caspase of sf9. however, it was not until 2013 that the first initiator caspase in sf9, sfdronc, was discovered, and the apoptotic pathway in sf9 became clearer. in this study, we report another caspase of sf9, sfdredd. sfdredd is ...	2016	26977926
identification of a tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.	the saliva of tsetse flies contains a cocktail of bioactive molecules inducing specific antibody responses in hosts exposed to bites. we have previously shown that an indirect-elisa test using whole salivary extracts from glossina morsitans submorsitans was able to discriminate between (i) cattle from tsetse infested and tsetse free areas and (ii) animals experimentally exposed to low or high numbers of tsetse flies. in the present study, our aim was to identify specific salivary synthetic pepti ...	2016	26979518
ambivalent outcomes of cell apoptosis: a barrier or blessing in malaria progression.	the life cycle of plasmodium in two evolutionary distant hosts, mosquito, and human, is a complex process. it is regulated at various stages of developments by a number of diverged mechanisms that ultimately determine the outcome of the disease. during the development processes, plasmodium invades a variety of cells in two hosts. the invaded cells tend to undergo apoptosis and are subsequently removed from the system. this process also eliminates numerous parasites along with these apoptotic cel ...	2016	27014225
factors associated with correct and consistent insecticide treated curtain use in iquitos, peru.	dengue is an arthropod-borne virus of great public health importance, and control of its mosquito vectors is currently the only available method for prevention. previous research has suggested that insecticide treated curtains (itcs) can lower dengue vector infestations in houses. this observational study investigated individual and household-level socio-demographic factors associated with correct and consistent use of itcs in iquitos, peru. a baseline knowledge, attitudes, and practices (kap) s ...	2016	26967157
integrating transgenic vector manipulation with clinical interventions to manage vector-borne diseases.	many vector-borne diseases lack effective vaccines and medications, and the limitations of traditional vector control have inspired novel approaches based on using genetic engineering to manipulate vector populations and thereby reduce transmission. yet both the short- and long-term epidemiological effects of these transgenic strategies are highly uncertain. if neither vaccines, medications, nor transgenic strategies can by themselves suffice for managing vector-borne diseases, integrating these ...	2016	26962871
rnai trigger delivery into anopheles gambiae pupae.	rna interference (rnai), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory for functional genomic studies. the ability to knockdown individual genes selectively via this reverse genetic technique has allowed many researchers to rapidly uncover the biological roles of numerous genes within many organisms, by evaluation of loss-of-function phenotypes. in the major human malaria vector anopheles gambiae, the predominant me ...	2016	27023367
amino acids trigger down-regulation of superoxide via torc pathway in the midgut of rhodnius prolixus.	sensing incoming nutrients is an important and critical event for intestinal cells to sustain life of the whole organism. the torc is a major protein complex involved in monitoring the nutritional status and is activated by elevated amino acid concentrations. an important feature of haematophagy is that huge amounts of blood are ingested in a single meal, which results in the release of large quantities of amino acids, together with the haemoglobin prosthetic group, haem, which decomposes hydrop ...	2016	26945025
immunodominant dengue virus-specific cd8+ t cell responses are associated with a memory pd-1+ phenotype.	dengue disease is a large public health problem that mainly afflicts tropical and subtropical regions. understanding of the correlates of protection against dengue virus (denv) is poor and hinders the development of a successful human vaccine. the present study aims to define denv-specific cd8(+)t cell responses in general and those of hla alleles associated with dominant responses in particular. in human blood donors in nicaragua, we observed a striking dominance of hla b-restricted responses i ...	2016	26912627
[hemorrhagic form of dengue fever observed at the infectious diseases department chu yalgado ouédraogo, burkina faso].	dengue is a re-emerging arboviral disease in tropical countries. haemorrhagic form may be associated with circulatory failure and produce hypovolemic shock, which is often fatal. we report a series of three cases of hemorrhagic dengue observed at the infectious diseases department chu yalgado ouédraogo, ouagadougou, in order to describe its epidemiological, clinical, and evolutive characteristics. our study included young females who had an onset age of between 35 years and 45 years, residing in ...	2016	27303584
indole alkaloids of the stigonematales (cyanophyta): chemical diversity, biosynthesis and biological activity.	the cyanobacteria are well recognized as producers of a wide array of bioactive metabolites including toxins, and potential drug candidates. however, a limited number of taxa are generally considered with respect to both of these aspects. that said, the order stigonematales, although largely overlooked in this regard, has become increasingly recognized as a source of bioactive metabolites relevant to both human and environmental health. in particular, the hapalindoles and related indole alkaloid ...	2016	27058546
polyandry depends on postmating time interval in the dengue vector aedes aegypti.	aedes aegypti is the primary vector of the dengue and chikungunya viruses. after mating, male seminal fluid molecules cause females to become unreceptive to a subsequent mating. this response is often assumed to be immediate and complete, but a growing body of evidence suggests that some females do mate more than once. it is unknown how quickly a female becomes unreceptive to a second mating. furthermore, the degree to which she remains monandrous after laying several batches of eggs has not bee ...	2016	26880776
mathematical model of three age-structured transmission dynamics of chikungunya virus.	we developed a new age-structured deterministic model for the transmission dynamics of chikungunya virus. the model is analyzed to gain insights into the qualitative features of its associated equilibria. some of the theoretical and epidemiological findings indicate that the stable disease-free equilibrium is globally asymptotically stable when the associated reproduction number is less than unity. furthermore, the model undergoes, in the presence of disease induced mortality, the phenomenon of ...	2016	27190548
phytochemical composition, antifungal and antioxidant activity of duguetia furfuracea a. st.-hill.	background. duguetia furfuracea is popular plant used in popular medicine. hypothesis/purpose. this claim evaluated the phytochemical composition of the hydroethanolic extract (hedf), fractions of duguetia furfuracea, and antioxidant and antifungal activity. methods. the chemical profile was carried out by hplc-dad. the total phenolic contents and flavonoid components were determined by folin-ciocalteu and aluminium chloride reaction. the antioxidant activity was measured by scavenging of 2,2-di ...	2016	27127550
vaccination of gerbils with bm-103 and bm-ral-2 concurrently or as a fusion protein confers consistent and improved protection against brugia malayi infection.	the brugia malayi bm-103 and bm-ral-2 proteins are orthologous to onchocerca volvulus ov-103 and ov-ral-2, and which were selected as the best candidates for the development of an o. volvulus vaccine. the b. malayi gerbil model was used to confirm the efficacy of these ov vaccine candidates on adult worms and to determine whether their combination is more efficacious.	2016	27045170
efficacy of four commercially available heartworm preventive products against the jyd-34 laboratory strain of dirofilaria immitis.	heartworm disease in dogs can be severe and life threatening. resistance to available heartworm preventives was considered among potential causes of increased reports of failed heartworm prevention in dogs. the objective of the present study was to compare the efficacy of four commercially available heartworm disease preventives against the jyd-34 strain of d. immitis.	2016	27044379
evaluating the effectiveness of localized control strategies to curtail chikungunya.	chikungunya, a re-emerging arbovirus transmitted to humans by aedes aegypti and ae. albopictus mosquitoes, causes debilitating disease characterized by an acute febrile phase and chronic joint pain. chikungunya has recently spread to the island of st. martin and subsequently throughout the americas. the disease is now affecting 42 countries and territories throughout the americas. while chikungunya is mainly a tropical disease, the recent introduction and subsequent spread of ae. albopictus into ...	2016	27045523
climate change and aedes vectors: 21st century projections for dengue transmission in europe.	warming temperatures may increase the geographic spread of vector-borne diseases into temperate areas. although a tropical mosquito-borne viral disease, a dengue outbreak occurred in madeira, portugal, in 2012; the first in europe since 1920s. this outbreak emphasizes the potential for dengue re-emergence in europe given changing climates. we present estimates of dengue epidemic potential using vectorial capacity (vc) based on historic and projected temperature (1901-2099). vc indicates the vect ...	2016	27322480
a novel video-tracking system to quantify the behaviour of nocturnal mosquitoes attacking human hosts in the field.	many vectors of malaria and other infections spend most of their adult life within human homes, the environment where they bloodfeed and rest, and where control has been most successful. yet, knowledge of peri-domestic mosquito behaviour is limited, particularly how mosquitoes find and attack human hosts or how insecticides impact on behaviour. this is partly because technology for tracking mosquitoes in their natural habitats, traditional dwellings in disease-endemic countries, has never been a ...	2016	27075002
first identification of kdr allele f1534s in vgsc gene and its association with resistance to pyrethroid insecticides in aedes albopictus populations from haikou city, hainan island, china.	aedes albopictus is distributed widely in china, as a primary vector of dengue fever and chikungunya fever in south of china. chemical insecticide control is one of the integrated programmes to prevent mosquito-borne diseases. long-term applications of pyrethroids have resulted in the development of resistance in ae. albopictus populations in china. however, the susceptibility of ae. albopictus to pyrethroids in hainan island was unclear. knockdown resistance (kdr), caused by point mutations in ...	2016	27133234
spatial and temporal country-wide survey of temephos resistance in brazilian populations of aedes aegypti.	the organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (aedes aegypti) in brazil since the mid-1980s. reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. as resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in ae. aegypti in br ...	2016	27143489
multiple evolutionary selections involved in synonymous codon usages in the streptococcus agalactiae genome.	streptococcus agalactiae is an important human and animal pathogen. to better understand the genetic features and evolution of s. agalactiae, multiple factors influencing synonymous codon usage patterns in s. agalactiae were analyzed in this study. a- and u-ending rich codons were used in s. agalactiae function genes through the overall codon usage analysis, indicating that adenine (a)/thymine (t) compositional constraints might contribute an important role to the synonymous codon usage pattern. ...	2016	26927064
a disease around the corner.		2016	26981335
a primary screening and applying of plant volatiles as repellents to control whitefly bemisia tabaci (gennadius) on tomato.	with the goal of finding a new way to reduce population densities of bemisia tabaci biotype q in greenhouses, seven repellent volatile chemicals and their combinations were screened. the mixture of dlco (d-limonene, citral and olive oil (63:7:30)) had a better cost performance(sc50 = 22.59 mg/ml)to repel whiteflies from settling than the other mixtures or single chemicals. in the greenhouse, in both the choice test and the no-choice tests, the number of adult whiteflies that settled on 1% dlco-t ...	2016	26907368
changes in the physicochemical properties of piperine/β-cyclodextrin due to the formation of inclusion complexes.	piperine (pp) is a pungent component in black pepper that possesses useful biological activities; however it is practically insoluble in water. the aim of the current study was to prepare a coground mixture (gm) of pp and β-cyclodextrin (βcd) (molar ratio of pp/βcd = 1/1) and subsequently evaluate the solubility of pp and physicochemical properties of the gm. dsc thermal behavior of the gm showed the absence of melting peak of piperine. pxrd profile of the gm exhibited halo pattern and no charac ...	2016	26998357
insight into the mode of action of haedoxan a from phryma leptostachya.	haedoxan a (ha) is a major active ingredient in the herbaceous perennial plant lopseed (phryma leptostachya l.), which is used as a natural insecticide against insect pests in east asia. here, we report that ha delayed the decay rate of evoked excitatory junctional potentials (ejps) and increased the frequency of miniature ejps (mejps) on the drosophila neuromuscular junction. ha also caused a significant hyperpolarizing shift of the voltage dependence of fast inactivation of insect sodium chann ...	2016	26907348
chemical diversity and biological properties of secondary metabolites from sea hares of aplysia genus.	the marine environment is an important source of structurally-diverse and biologically-active secondary metabolites. during the last two decades, thousands of compounds were discovered in marine organisms, several of them having inspired the development of new classes of therapeutic agents. marine mollusks constitute a successful phyla in the discovery of new marine natural products (mnps). over a 50-year period from 1963, 116 genera of mollusks contributed innumerous compounds, aplysia being th ...	2016	26907303
developing a time series predictive model for dengue in zhongshan, china based on weather and guangzhou dengue surveillance data.	dengue is a re-emerging infectious disease of humans, rapidly growing from endemic areas to dengue-free regions due to favorable conditions. in recent decades, guangzhou has again suffered from several big outbreaks of dengue; as have its neighboring cities. this study aims to examine the impact of dengue epidemics in guangzhou, china, and to develop a predictive model for zhongshan based on local weather conditions and guangzhou dengue surveillance information.	2016	26894570
differential juvenile hormone variations in scale insect extreme sexual dimorphism.	scale insects have evolved extreme sexual dimorphism, as demonstrated by sedentary juvenile-like females and ephemeral winged males. this dimorphism is established during the post-embryonic development; however, the underlying regulatory mechanisms have not yet been examined. we herein assessed the role of juvenile hormone (jh) on the diverging developmental pathways occurring in the male and female japanese mealybug planococcus kraunhiae (kuwana). we provide, for the first time, detailed gene e ...	2016	26894583
transcriptome analysis and discovery of genes relevant to development in bradysia odoriphaga at three developmental stages.	bradysia odoriphaga (diptera: sciaridae) is the most important pest of chinese chive (allium tuberosum) in asia; however, the molecular genetics are poorly understood. to explore the molecular biological mechanism of development, illumina sequencing and de novo assembly were performed in the third-instar, fourth-instar, and pupal b. odoriphaga. the study resulted in 16.2 gb of clean data and 47,578 unigenes (≥125 bp) contained in 7,632,430 contigs, 46.21% of which were annotated from non-redunda ...	2016	26891450
establishment of a wolbachia superinfection in aedes aegypti mosquitoes as a potential approach for future resistance management.	wolbachia pipientis is an endosymbiotic bacterium estimated to chronically infect between 40-75% of all arthropod species. aedes aegypti, the principle mosquito vector of dengue virus (denv), is not a natural host of wolbachia. the transinfection of wolbachia strains such as walbb, wmel and wmelpop-cla into ae. aegypti has been shown to significantly reduce the vector competence of this mosquito for a range of human pathogens in the laboratory. this has led to wmel-transinfected ae. aegypti curr ...	2016	26891349
deep rna-seq profile reveals biodiversity, plant-microbe interactions and a large family of nbs-lrr resistance genes in walnut (juglans regia) tissues.	deep rna-seq profiling, a revolutionary method used for quantifying transcriptional levels, often includes non-specific transcripts from other co-existing organisms in spite of stringent protocols. using the recently published walnut genome sequence as a filter, we present a broad analysis of the rna-seq derived transcriptome profiles obtained from twenty different tissues to extract the biodiversity and possible plant-microbe interactions in the walnut ecosystem in california. since the residua ...	2016	26883051
large-scale modelling of the environmentally-driven population dynamics of temperate aedes albopictus (skuse).	the asian tiger mosquito, aedes albopictus, is a highly invasive vector species. it is a proven vector of dengue and chikungunya viruses, with the potential to host a further 24 arboviruses. it has recently expanded its geographical range, threatening many countries in the middle east, mediterranean, europe and north america. here, we investigate the theoretical limitations of its range expansion by developing an environmentally-driven mathematical model of its population dynamics. we focus on t ...	2016	26871447
climate and the timing of imported cases as determinants of the dengue outbreak in guangzhou, 2014: evidence from a mathematical model.	as the world's fastest spreading vector-borne disease, dengue was estimated to infect more than 390 million people in 2010, a 30-fold increase in the past half century. although considered to be a non-endemic country, mainland china had 55,114 reported dengue cases from 2005 to 2014, of which 47,056 occurred in 2014. furthermore, 94% of the indigenous cases in this time period were reported in guangdong province, 83% of which were in guangzhou city. in order to determine the possible determinant ...	2016	26863623
conservation, duplication, and divergence of five opsin genes in insect evolution.	opsin proteins covalently bind to small molecular chromophores and each protein-chromophore complex is sensitive to particular wavelengths of light. multiple opsins with different wavelength absorbance peaks are required for color vision. comparing opsin responses is challenging at low light levels, explaining why color vision is often lost in nocturnal species. here, we investigated opsin evolution in 27 phylogenetically diverse insect species including several transitions between photic niches ...	2016	26865071
novel factors of anopheles gambiae haemocyte immune response to plasmodium berghei infection.	insect haemocytes mediate cellular immune responses (e.g., phagocytosis) and contribute to the synthesis of humoral immune factors. in previous work, a genome-wide molecular characterization of anopheles gambiae circulating haemocytes was followed by functional gene characterization using cell-based rnai screens. assays were carried out to investigate the role of selected haemocyte-specific or enriched genes in phagocytosis of bacterial bio-particles, expression of the antimicrobial peptide cecr ...	2016	26858200
first report of y-linked genes in the kissing bug rhodnius prolixus.	due to an abundance of repetitive dna, the annotation of heterochromatic regions of the genome such as the y chromosome is problematic. the y chromosome is involved in key biological functions such as male-fertility and sex-determination and hence, accurate identification of its sequences is vital. the hemipteran insect rhodnius prolixus is an important vector of chagas disease, a trypanosomiasis affecting 6-7 million people worldwide. here we report the identification of the first y-linked gene ...	2016	26861771
insulin-related peptide 5 is involved in regulating embryo development and biochemical composition in pea aphid with wing polyphenism.	in aphids there is a fecundity-dispersal trade-off between wingless and winged morphs. recent research on the molecular mechanism of wing morphs associated with dispersal reveals that insulin receptors in the insulin signaling (is) pathway regulate alternation of wing morphs in planthoppers. however, little is known about whether genes in the is pathway are involved in developmental regulation in aphid nymphs with different wing morphs. in this study, we show that expression of the insulin-relat ...	2016	26903881
spermatogenesis drives rapid gene creation and masculinization of the x chromosome in stalk-eyed flies (diopsidae).	throughout their evolutionary history, genomes acquire new genetic material that facilitates phenotypic innovation and diversification. developmental processes associated with reproduction are particularly likely to involve novel genes. abundant gene creation impacts the evolution of chromosomal gene content and general regulatory mechanisms such as dosage compensation. numerous studies in model organisms have found complex and, at times contradictory, relationships among these genomic attribute ...	2016	26951781
rna sequencing to characterize transcriptional changes of sexual maturation and mating in the female oriental fruit fly bactrocera dorsalis.	female reproductive potential plays a significant role in the survival and stability of species, and sexual maturation and mating processes are crucial. however, our knowledge of the reproductive genes involved in sexual maturation and mating has been largely limited to model organisms. the oriental fruit fly bactrocera dorsalis is a highly invasive agricultural pest, known to cause major economic losses; thus, it is of great value to understand the transcriptional changes involved in sexual mat ...	2016	26946038
identification of autophagy in the pine wood nematode bursaphelenchus xylophilus and the molecular characterization and functional analysis of two novel autophagy-related genes, bxatg1 and bxatg8.	the pine wood nematode, bursaphelenchus xylophilus, causes huge economic losses in pine forests, has a complex life cycle, and shows the remarkable ability to survive under unfavorable and changing environmental conditions. this ability may be related to autophagy, which is still poorly understood in b. xylophilus and no autophagy-related genes have been previously characterized. in this study, transmission electron microscopy was used to confirm that autophagy exists in b. xylophilus. the full- ...	2016	26950119
distribution of voltage-gated sodium channel (nav) alleles among the aedes aegypti populations in central java province and its association with resistance to pyrethroid insecticides.	the emergence of insecticide resistant aedes aegypti mosquitoes has hampered dengue control efforts. who susceptibility tests, using several pyrethroid compounds, were conducted on ae. aegypti larvae that were collected and raised to adulthood from semarang, surakarta, kudus and jepara in java. the aanav gene fragment encompassing kdr polymorphic sites from both susceptible and resistant mosquitoes was amplified, and polymorphisms were associated with the resistant phenotype. the insecticide sus ...	2016	26939002
spatial and temporal epidemiology of lumpy skin disease in the middle east, 2012-2015.	lumpy skin disease virus (lsdv) is an infectious disease of cattle that can have severe economic implications. new lsd outbreaks are currently circulating in the middle east (me). since 2012, severe outbreaks were reported in cattle across the region. characterizing the spatial and temporal dynamics of lsdv in cattle populations is prerequisite for guiding successful surveillance and control efforts at a regional level in the me. here, we aim to model the ecological niche of lsdv and identify ep ...	2016	26973845
a comprehensive entomological, serological and molecular study of 2013 dengue outbreak of swat, khyber pakhtunkhwa, pakistan.	aedes aegypti and aedes albopictus play a fundamental role in transmission of dengue virus to humans. a single infected aedes mosquito is capable to act as a reservoir/amplifier host for dengue virus and may cause epidemics via horizontal and vertical modes of dengue virus (denv) transmission. the present and future dengue development can be clarified by understanding the elements which help the dissemination of dengue transmission. the current study deals with molecular surveillance of dengue i ...	2016	26848847
co-expression of dorsal and rel2 negatively regulates antimicrobial peptide expression in the tobacco hornworm manduca sexta.	nuclear factor κb (nf-κb) plays an essential role in regulation of innate immunity. in mammals, nf-κb factors can form homodimers and heterodimers to activate gene expression. in insects, three nf-κb factors, dorsal, dif and relish, have been identified to activate antimicrobial peptide (amp) gene expression. however, it is not clear whether dorsal (or dif) and relish can form heterodimers. here we report the identification and functional analysis of a dorsal homologue (msdorsal) and two relish ...	2016	26847920
the spermatophore in glossina morsitans morsitans: insights into male contributions to reproduction.	male seminal fluid proteins (sfps) transferred during copulation modulate female reproductive physiology and behavior, impacting sperm storage/use, ovulation, oviposition, and remating receptivity. these capabilities make them ideal targets for developing novel methods of insect disease vector control. little is known about the nature of sfps in the viviparous tsetse flies (diptera: glossinidae), vectors of human and animal african trypanosomiasis. in tsetse, male ejaculate is assembled into a c ...	2016	26847001
newly emerging parasitic threats for human health: national and international trends.		2016	26966685
a new allele conferring resistance to lysinibacillus sphaericus is detected in low frequency in culex quinquefasciatus field populations.	the cqm1 α-glucosidase of culex quinquefasciatus larvae acts as the midgut receptor for the binary toxin of the biolarvicide lysinibacillus sphaericus. mutations within the cqm1 gene can code for aberrant polypeptides that can no longer be properly expressed or bind to the toxin, leading to insect resistance. the cqm1 rec and cqm1 rec-2 alleles were identified in a laboratory selected colony and both displayed mutations that lead to equivalent phenotypes of refractoriness to l. sphaericus. cqm1 ...	2016	26846600
spatial variation of insecticide resistance in the dengue vector aedes aegypti presents unique vector control challenges.	dengue is a major public health problem in mexico, where the use of chemical insecticides to control the principal dengue vector, aedes aegypti, is widespread. resistance to insecticides has been reported in multiple sites, and the frequency of kdr mutations associated with pyrethroid resistance has increased rapidly in recent years. in the present study, we characterized patterns of insecticide resistance in ae. aegypti populations in five small towns surrounding the city of merida, mexico.	2016	26846468
development of a novel-type transgenic cotton plant for control of cotton bollworm.	the transgenic bt cotton plant has been widely planted throughout the world for the control of cotton budworm helicoverpa armigera (hubner). however, a shift towards insect tolerance of bt cotton is now apparent. in this study, the gene encoding neuropeptide f (npf) was cloned from cotton budworm h. armigera, an important agricultural pest. the npf gene produces two splicing mrna variants-npf1 and npf2 (with a 120-bp segment inserted into the npf1 sequence). these are predicted to form the matur ...	2016	26841044
finding aedes aegypti in a natural breeding site in an urban zone, sao paulo, southeastern brazil.	this is the description of how nine aedes aegypti larvae were found in a natural breeding site in the pinheiros neighborhood, city of sao paulo, sp, southeastern brazil. the record was conducted in december 2014, during an entomological surveillance program of dengue virus vectors, with an active search of potential breeding sites, either artificial or natural. findingae. aegypti larvae in a tree hole shows this species' ability to use both artificial and natural environments as breeding sites a ...	2016	26982959
evaluation of the activity of the essential oil from an ornamental flower against aedes aegypti: electrophysiology, molecular dynamics and behavioral assays.	dengue fever has spread worldwide and affects millions of people every year in tropical and subtropical regions of africa, asia, europe and america. since there is no effective vaccine against the dengue virus, prevention of disease transmission depends entirely on regulating the vector (aedes aegypti) or interrupting human-vector contact. the aim of this study was to assess the oviposition deterrent activity of essential oils of three cultivars of torch ginger (etlingera elatior, zingiberaceae) ...	2016	26927837
nutritional signaling regulates vitellogenin synthesis and egg development through juvenile hormone in nilaparvata lugens (stål).	insect female reproduction which comprises the synthesis of vitellogenein (vg) in the fat body and its incorporation into developing oocytes, needs a large amount of energy and food resources. our previous studies found that juvenile hormone (jh) regulates vitellogenesis in the brown planthopper, nilaparvata lugens. here, we report on the role of jh in nutrient-regulated vg synthesis and egg development. we first cloned the genes coding for juvenile hormone acid methyltransferase (jhamt) which i ...	2016	26927076
evolution of the assassin's arms: insights from a phylogeny of combined transcriptomic and ribosomal dna data (heteroptera: reduvioidea).	assassin bugs (reduvioidea) are one of the most diverse (>7,000 spp.) lineages of predatory animals and have evolved an astounding diversity of raptorial leg modifications for handling prey. the evolution of these modifications is not well understood due to the lack of a robust phylogeny, especially at deeper nodes. we here utilize refined data from transcriptomes (370 loci) to stabilize the backbone phylogeny of reduvioidea, revealing the position of major clades (e.g., the chagas disease vecto ...	2016	26916580
altered carbohydrates allocation by associated bacteria-fungi interactions in a bark beetle-microbe symbiosis.	insect-microbe interaction is a key area of research in multiplayer symbiosis, yet little is known about the role of microbe-microbe interactions in insect-microbe symbioses. the red turpentine beetle (rtb) has destroyed millions of healthy pines in china and forms context-dependent relationships with associated fungi. the adult-associated fungus leptographium procerum have played key roles in rtb colonization. however, common fungal associates (l. procerum and ophiostoma minus) with rtb larvae ...	2016	26839264
microrna profiling of the whitefly bemisia tabaci middle east-aisa minor i following the acquisition of tomato yellow leaf curl china virus.	the begomoviruses are the largest and most economically important group of plant viruses exclusively vectored by whitefly (bemisia tabaci) in a circulative, persistent manner. during this process, begomoviruses and whitefly vectors have developed close relationships and complex interactions. however, the molecular mechanisms underlying these interactions remain largely unknown, and the microrna profiles for viruliferous and nonviruliferous whiteflies have not been studied.	2016	26837429
serological and entomological study of dengue in dang and chitwan districts of nepal.	a descriptive cross-sectional study was carried out among 264 suspected dengue patients in two districts (dang and chitwan) of nepal from june 2013 to november 2013. the anti-dengue igm positivity was found to be (51/264)19.31% by capture elisa, of which 21 (41.2%) were male and 30 (58.8%) were female. symptoms of seropositive cases were fever, anorexia, nausea, headache, retro-orbital pain, skin rashes, and myalgia. hematological features like thrombocytopenia and leucopenia were found to be si ...	2016	26828951
reference genes for accessing differential expression among developmental stages and analysis of differential expression of obp genes in anastrepha obliqua.	the west indian fruit fly, anastrepha obliqua, is an important agricultural pest in the new world. the use of pesticide-free methods to control invasive species such as this reinforces the search for genes potentially useful in their genetic control. therefore, the study of chemosensory proteins involved with a range of responses to the chemical environment will help not only on the understanding of the species biology but may also help the development of environmentally friendly pest control st ...	2016	26818909
toxic effect of blood feeding in male mosquitoes.	blood- and sugar feeding of female mosquitoes has been frequently observed in the laboratory and in the field, but only sugar feeding of males has been reported. here, we describe for the first time that culex quinquefasciatus males feed on blood as well. blood feeding easily happened on a blood-soaked cotton roll and, to a lesser extent, through a thin artificial layer. mating history of a male specimen does not affect his blood feeding behavior. male mosquitoes feed on blood even when they hav ...	2016	26858651
the spread of dengue in an endemic urban milieu--the case of delhi, india.	dengue is a major international public health concern, one of the most important arthropod-borne diseases. more than 3.5 billion people are at risk of dengue infection and there are an estimated 390 million dengue infections annually. this prolific increase has been connected to societal changes such as population growth and increasing urbanization generating intense agglomeration leading to proliferation of synanthropic mosquito species. quantifying the spatio-temporal epidemiology of dengue in ...	2016	26808518
comprehensive immunolocalization studies of a putative serotonin receptor from the alimentary canal of aedes aegypti larvae suggest its diverse roles in digestion and homeostasis.	serotonin regulates key processes including digestion and homeostasis in insects. serotonin effects are mediated by serotonin receptors that transduce information through initiation of second messenger signaling pathways. lack of information on serotonin receptors associated with the alimentary canal impedes the understanding of the serotonergic role in insect physiology. to address this void, the present study has cloned and identified a putative serotonin receptor (hereafter aaser-1) from the ...	2016	26808995
towards plant species identification in complex samples: a bioinformatics pipeline for the identification of novel nuclear barcode candidates.	monitoring of the food chain to fight fraud and protect consumer health relies on the availability of methods to correctly identify the species present in samples, for which dna barcoding is a promising candidate. the nuclear genome is a rich potential source of barcode targets, but has been relatively unexploited until now. here, we show the development and use of a bioinformatics pipeline that processes available genome sequences to automatically screen large numbers of input candidates, ident ...	2016	26807711
something "hairy" in juvenile hormone signaling for mosquito reproduction.		2016	26802123
accurate transposable element annotation is vital when analyzing new genome assemblies.	transposable elements (tes) are mobile genetic elements with the ability to replicate themselves throughout the host genome. in some taxa tes reach copy numbers in hundreds of thousands and can occupy more than half of the genome. the increasing number of reference genomes from nonmodel species has begun to outpace efforts to identify and annotate te content and methods that are used vary significantly between projects. here, we demonstrate variation that arises in te annotations when less than ...	2016	26802115
serotonergic innervation of the salivary glands and central nervous system of adult glossina pallidipes austen (diptera: glossinidae), and the impact of the salivary gland hypertrophy virus (gpsghv) on the host.	using a serotonin antibody and confocal microscopy, this study reports for the first time direct serotonergic innervation of the muscle sheath covering the secretory region of the salivary glands of adult tsetse fly, glossina pallidipes austen. reports to date, however, note that up until this finding, dipteran species previously studied lack a muscle sheath covering of the secretory region of the salivary glands. direct innervation of the salivary gland muscle sheath of tsetse would facilitate ...	2016	26798144
insecticidal activity and insect repellency of four species of sea lily (comatulida: comatulidae) from taiwan.	the methanol and ethyl acetate (ea) extracts of four species of sea lily (himerometra magnipinna, comaster multifidus, comanthina sp., and comatella maculata) were evaluated for their insecticidal activity against yellow-fever mosquito larvae (aedes aegypti) and their repellency against adult asian tiger mosquitoes (aedes albopictus). the 24-hr minimum inhibition concentration (mic) data revealed that the extracts from h. magnipinna and the c. maculata were the most active, killing mosquito larv ...	2016	26798137
recent advances in understanding dengue.	dengue is an emerging threat to billions of people worldwide. in the last 20 years, the incidence has increased four-fold and this trend appears to be continuing. caused by one of four viral serotypes, dengue can present as a wide range of clinical phenotypes with the severe end of the spectrum being defined by a syndrome of capillary leak, coagulopathy, and organ impairment. the pathogenesis of severe disease is thought to be in part immune mediated, but the exact mechanisms remain to be define ...	2016	26918159
the iflaviruses sacbrood virus and deformed wing virus evoke different transcriptional responses in the honeybee which may facilitate their horizontal or vertical transmission.	sacbrood virus (sbv) and deformed wing virus (dwv) are evolutionarily related positive-strand rna viruses, members of the iflavirus group. they both infect the honeybee apis mellifera but have strikingly different levels of virulence when transmitted orally. honeybee larvae orally infected with sbv usually accumulate high levels of the virus, which halts larval development and causes insect death. in contrast, oral dwv infection at the larval stage usually causes asymptomatic infection with low ...	2016	26819848
viruses causing hemorrhagic fever. safety laboratory procedures.	viral hemorrhagic fevers are diseases caused by viruses which belong to different families, many of them causing severe diseases. these viruses may produce different symptomatology together with a severe multisystem syndrome, and the final result might be the production of hemorrhages in several sites of the body. the majority of them have no other treatment than supportive therapy, although some antiviral drugs can be used in some circumstances. transmission of vhf has been demonstrated through ...	2016	27014378
caste-specific and sex-specific expression of chemoreceptor genes in a termite.	the sophisticated colony organization of eusocial insects is primarily maintained through the utilization of pheromones. the regulation of these complex social interactions requires intricate chemoreception systems. the recent publication of the genome of zootermopsis nevadensis opened a new avenue to study molecular basis of termite caste systems. although there has been a growing interest in the termite chemoreception system that regulates their sophisticated caste system, the relationship bet ...	2016	26760975
effects of food variability on growth and reproduction of aedes aegypti.	despite a large body of knowledge about the evolution of life histories, we know little about how variable food availability during an individual's development affects its life history. we measured the effects of manipulating food levels during early and late larval development of the mosquito aedes aegypti on its growth rate, life history and reproductive success. switching from low to high food led to compensatory growth: individuals grew more rapidly during late larval development and emerged ...	2016	26843938
carbon dioxide receptor genes and their expression profile in diabrotica virgifera virgifera.	diabrotica virgifera virgifera, western corn rootworm, is one of the most devastating species in north america. d. v. virgifera neonates crawl through the soil to locate the roots on which they feed. carbon dioxide (co2) is one of the important volatile cues that attract d. v. virgifera larvae to roots.	2016	26746870
protection of dogs against canine heartworm infection 28 days after four monthly treatments with advantage multi® for dogs.	monthly heartworm preventives are designed to protect dogs by killing heartworms acquired the month prior to their administration, and after treatment with most products, the drug levels rapidly dissipate to very low levels. work with advantage multi® for dogs (imidacloprid + moxidectin) topical solution showed protection against hookworm infection throughout the month after administration of several monthly doses suggesting that similar protection might occur with heartworms. this study assesse ...	2016	26746229
effects of bmcpv infection on silkworm bombyx mori intestinal bacteria.	the gut microbiota has a crucial role in the growth, development and environmental adaptation in the host insect. the objective of our work was to investigate the microbiota of the healthy silkworm bombyx mori gut and changes after the infection of b. mori cypovirus (bmcpv). intestinal contents of the infected and healthy larvae of b. mori of fifth instar were collected at 24, 72 and 144 h post infection with bmcpv. the gut bacteria were analyzed by pyrosequencing of the 16s rrna gene. 147(135) ...	2016	26745627
costs of three wolbachia infections on the survival of aedes aegypti larvae under starvation conditions.	the mosquito aedes aegypti, the principal vector of dengue virus, has recently been infected experimentally with wolbachia: intracellular bacteria that possess potential as dengue biological control agents. wolbachia depend on their hosts for nutrients they are unable to synthesize themselves. consequently, competition between wolbachia and their host for resources could reduce host fitness under the competitive conditions commonly experienced by larvae of ae. aegypti in the field, hampering the ...	2016	26745630
hairy and groucho mediate the action of juvenile hormone receptor methoprene-tolerant in gene repression.	the arthropod-specific juvenile hormone (jh) controls numerous essential functions. its involvement in gene activation is known to be mediated by the transcription factor methoprene-tolerant (met), which turns on jh-controlled genes by directly binding to e-box-like motifs in their regulatory regions. however, it remains unclear how jh represses genes. we used the aedes aegypti female mosquito, in which jh is necessary for reproductive maturation, to show that a repressor, hairy, is required for ...	2016	26744312