Publications

septic pylephlebitis following shigella bacteremia: case report.		1986	3535150
morphologic evaluation of the effects of shiga toxin and e coli shiga-like toxin on the rabbit intestine.	the effects of a shiga toxin derived from shigella dysenteriae type 1, strain 60r, and a shiga-like toxin from the enterohemorrhagic escherichia coli o157:h7, strain 933, were studied in the in vivo rabbit ileal loop model. the effects of both toxins were similar and resulted in severe villus blunting by 18-24 hours after exposure. with both toxins, a dose effect was noted; and the lesions, first detected at 2 hours after inoculation, became more severe over time. both toxins appeared to act dir ...	1986	3535529
shigella dysenteriae type 1 enterocolitis.	shigella dysenteriae type 1 is much more virulent than shigella flexneri and sonnei which are endemic in australia. this report describes a 22 year old woman who acquired shigella dysenteriae type 1 whilst travelling in india. during the course of her illness, she developed severe enterocolitis for which a subtotal colectomy was performed. the illness resembled fulminant ulcerative colitis and its infectious nature was difficult to establish because several fecal cultures failed to grow the path ...	1986	3535768
production of shiga toxin and other cytotoxins by serogroups of shigella.	we measured the cytotoxic activity of 119 strains of shigella by using a quantitative [3h]thymidine-labeled hela cell assay. we assayed 13 strains of shigella dysenteriae 1; 18 strains of s. dysenteriae types 2 and 3; and 88 strains of shigella sonnei, shigella flexneri, and shigella boydii. strains of s. dysenteriae 1 demonstrated high levels of cytotoxicity (geometric mean, 10(5.04) cd50/mg of protein; range, 10(3.95)-10(6.10). cytotoxic activities of the non-type 1 strains of s. dysenteriae a ...	1986	3537154
plasmid-mediated contact haemolytic activity in shigella species: correlation with penetration into hela cells.	the main feature of virulent strains of shigella is their ability to invade eucaryotic cells. this phenotype is both plasmid-mediated and temperature-regulated. in the present report, we demonstrate a plasmid-mediated and temperature-regulated haemolytic activity in s. flexneri, s. dysenteriae and s. sonnei. detection of this haemolytic activity requires centrifugation of suspensions containing bacteria and erythrocytes, followed by incubation of the pellets at 37 degrees c. no soluble intra- or ...	1986	3322171
synthetic peptides of shiga toxin b-subunit induce antibodies which neutralize the biological activities of the toxin.		1986	3322336
shiga toxin from shigella dysenteriae 1 inhibits protein synthesis in reticulocyte lysates by inactivation of aminoacyl-trna binding.	inhibition of the peptide elongation cycle of eukaryotic protein synthesis by shiga toxin from shigella dysenteriae 1 was examined in toxin-treated reticulocyte lysate mixtures. peptidyl transferase activity of toxin-treated ribosomes was measured by following the decrease in peptidyl-trna concentrations when puromycin was added after incubation with toxin. concentrations of [3h]leucine-labeled peptidyl-trna were measured by extraction with cetyltrimethylammonium bromide. the data suggest that s ...	1986	3334156
osteogenic sarcoma and soft tissue myxoma in a patient with fibrous dysplasia and hemoglobins jbaltimore and s.	a 41-year-old man with recognized polyostotic fibrous dysplasia since late childhood developed fibroblastic osteogenic sarcoma in the left tibia. four months after the initial diagnosis, an intramuscular myxoma was discovered in the left thigh. twenty years previously he had been found to be heterozygous for hemoglobins jbaltimore and s. malignant transformation in fibrous dysplasia is unusual and may be associated in some individuals with prior irradiation. soft tissue myxomas associated with f ...	1986	3456858
distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins.	the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ...	1986	3944102
distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins.	the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ...	1986	3944102
comparison of particle-associated progesterone and oxytocin in the ovine corpus luteum.	the subcellular distribution of progesterone and oxytocin within the ovine corpus luteum was investigated using differential and density gradient centrifugation. progesterone and oxytocin were associated with particles which sedimented to a density of 1.049-1.054 g/ml and 1.054-1.061 g/ml respectively. particle-associated progesterone did not, however, display physical or biochemical characteristics consistent with its storage within secretory granules. when particle-associated progesterone was ...	1986	3944530
[a preliminary study on the usefulness of the plasmid profile analysis for the epidemiological investigation of shigellosis].		1986	3545491
antibodies to rat liver cathepsins: characterization and use for the identification of enzyme precursors.		1986	3579871
outbreak of post-measles gastroenteritis due to shigella dysenteriae type-i in karsog tehsil, district mandi (h.p).		1986	3543110
[bacteriological study of shigellosis in the lake kivu area (central africa). developments in the last 15 years (1968-1983)].	1913 shigella strains were isolated from 1968 to 1983 in lake kivu area (central africa). distribution and evolution of serotypes, biotypes and antibiotypes were studied. the majority of serotypes and biotypes were stable. however, few of these strains showed variations, among them shigella dysenteriae serotype 1, causing epidemic dysenteriae since 1981. epidemiologic survey of shigellosis in kivu seems to be rather underdeveloped (4 bacteriology laboratories for an area of 10 million inhabitant ...	1986	3542256
immune responses to h-2kd antigen expressed by recombinant vaccinia virus.	a recombinant vaccinia virus (vv-h2kd-6) containing the coding sequence for the murine major histocompatibility complex class i antigen h-2kd has been constructed and used to express h-2kd on the surface of infected cells. vaccinia expressed h-2kd has been shown to generate an h-2kd-specific primary cytotoxic t-cell response in mice infected with the recombinant virus and to stimulate an h-2kd-specific cytotoxic t-cell response in vitro. cells infected with the recombinant virus acted as targets ...	1986	3489941
a case of human b cell leukemia that implicates an autocrine mechanism in the abnormal growth of leu 1 b cells.	production of b cell growth factor (bcgf) from b-chronic lymphocytic leukemia (b-cll) cells was demonstrated. freshly isolated monoclonal b-cll cells expressed surface mu, delta, b1, and leu 1, but not ba (an antigen expressed only on activated b cells). upon stimulation with anti-igm, they secreted bcgf, which could act on anti-igm-stimulated autologous leukemic cells as well as anti-igm-stimulated normal b cells. cell lines established from these leukemic cells also constitutively secreted bcg ...	1986	3490495
dysentery: an overview. still problems to resolve.	this article reviews the global situation with regard to bacillary dysentery, with particular emphasis on findings from the recent epidemic in west bengal. infection is by the fecal-oral route and generally spread by person-to-person transmission. serious epidemics caused by the multiple-drug-resistant shigella shigae have occurred recently in bangladesh, somalia, south india, burma, sri lanka, nepal, bhutan, rwanda, and zaire. in each situation, dysentery attacked over 10% of the population ...	1986	12341664
characterisation of a dictyostelium discoideum dna fragment coding for a putative trnavalguu gene. evidence for a single transcription unit consisting of two overlapping class iii genes.	a genomic dna fragment from dictyostelium discoideum was characterized. this dna, although 74% d(a + t)-rich, codes for a putative trnavalguu. the trnaval gene overlaps at its 5' half with another rna polymerase iii transcription unit. this rna polymerase iii transcription unit can be folded into a trna-like shape and is comprised of significant amounts of invariant and semi-invariant nucleotides present in all eukaryotic trnas. this unit contains the two promoter blocks defined for rna polymera ...	1985	3967666
noninvasive measurement of pressure/volume characteristics in human limb veins by electrical admittance plethysmography.		1985	3974317
effect of bromocriptine on serum pth, calcium and phosphate levels in azotemic rats.	the dopamine agonist, bromocriptine, was studied with respect to its effects on pth secretion and calcium homeostasis in sprague-dawley rats made azotemic by either total or subtotal nephrectomy. the oral or intraperitoneal administration of 0.25 mg of bromocriptine resulted in a significant increase in the serum calcium concentration when compared to animals given placebo. bromocriptine produced no significant change in the bun or the serum concentrations of creatinine, inorganic phosphate or p ...	1985	3979799
multiaxonal horizontal cells in the retina of the tree shrew, tupaia glis.	the retinas of most vertebrates contain two or more morphologically distinct types of horizontal cell, and usually one of these types lacks an axon. among mammals, in which two types are observed, primates are exceptional in that both types of horizontal cell have axons. it then seemed of interest to study the horizontal cells of tree shrews (tupaia glis), insectivores thought to be closely related to primates. golgi impregnations of whole, flat-preparations revealed two types of horizontal cell ...	1985	3980782
observations on facial nociception in a monkey after destruction of the rostral part of the trigeminal sensory nuclear complex.	facial sensibility was assessed in a cynomolgus monkey in whom the trigeminal main sensory nucleus and the rostral part of the trigeminal nucleus oralis on one side had been destroyed. the animal responded equally to noxious mechanical stimuli applied to the two sides of the face; no deficiency in nociception could be detected. this finding suggests that the synaptic connections made by nociceptor afferents from the face in rostral parts of the trigeminal sensory nuclear complex are not essentia ...	1985	3982839
epinephrine and norepinephrine syntheses are regulated by a glucocorticoid receptor-mediated mechanism in the bovine adrenal medulla.	the bovine adrenal medulla was investigated regarding the presence of glucocorticoid binding protein and the increases in ornithine decarboxylase (odc) activity and epinephrine and norepinephrine by dexamethasone. scatchard analysis of specific cytosol [3h] dexamethasone-binding study indicated a single class of high affinity (kd, 35 +/- 5 nm) and limited binding sites (150 +/- 26 fmoles/mg protein). competition studies of various steroids indicated a high affinity for dexamethasone and hydrocor ...	1985	3990518
[persistent chlamydia infection in cell culture].		1985	3993192
the acquired immunodeficiency syndrome (aids) in denmark. a report from the copenhagen study group of aids on the first 20 danish patients.	twenty danish patients with the acquired immunodeficiency syndrome (aids) had been diagnosed by january 1984, 14 of them after 1982. eighteen patients were male homosexuals, 8 of whom had visited the usa after 1979, 2 were heterosexual males with a history of sexual contacts in central africa, suggesting a transmission of aids from woman to man. aids has not been observed in drug abusers, hemophiliacs or transfused non-risk persons in denmark. the clinical picture varied according to the presenc ...	1985	3993435
[24-hour energy metabolism in the human: circadian rhythm, relation to body weight and nutrition].	in 7 men with normal weight and 9 man with overweight and healthy metabolism the resting and fasting energy expenditure was indirectly calorimetrically pursued in the open system over 24 hours. the total energy expenditure over 24 hours revealed an ascertained dependence on body-weight and nutrition. the persons with overweight had a higher absolute energy expenditure, however, the activity of the energy metabolism of the body mass free from fat and the active body mass, respectively, decreased ...	1985	4013439
[trial of an erythrocyte antigenic diagnostic agent for detecting antibodies to coxiella burnetii].	a new method for the preparation of q-fever erythrocyte antigenic diagnosticum, adaptable to large-scale production, has been developed, and the diagnosticum thus obtained has been found to be highly specific and sensitive. the combined use of the complement fixation and passive hemagglutination tests enhances the effectiveness of the serological study, as it not only ensures a more complete detection of antibodies to c. burnetii, phase i, in humans and cattle, but also gives more precise indica ...	1985	4013575
adaptation of skeletal muscle energy metabolism to repeated hypoxic-normoxic exposures and drug treatment.	muscular glycolytic fuels, intermediates and end-products (glycogen, glucose, glucose-6-phosphate, pyruvate, lactate), krebs cycle intermediates (citrate, alpha-ketoglutarate, succinate, malate), related free amino acids (glutamate, alanine), ammonia, energy store (creatine phosphate), energy mediators (atp, adp, amp) and energy charge potential were evaluated. furthermore the maximum rate (vmax) of the following muscular enzyme activities was evaluated in the crude extract and/or mitochondrial ...	1985	4015259
adaptation of skeletal muscle energy metabolism to repeated hypoxic-normoxic exposures and drug treatment.	muscular glycolytic fuels, intermediates and end-products (glycogen, glucose, glucose-6-phosphate, pyruvate, lactate), krebs cycle intermediates (citrate, alpha-ketoglutarate, succinate, malate), related free amino acids (glutamate, alanine), ammonia, energy store (creatine phosphate), energy mediators (atp, adp, amp) and energy charge potential were evaluated. furthermore the maximum rate (vmax) of the following muscular enzyme activities was evaluated in the crude extract and/or mitochondrial ...	1985	4015259
quantitative determination of tramadol in human serum by gas chromatography-mass spectrometry.	a gas chromatographic-mass spectrometric method for the quantitative determination of tramadol in human serum, plasma or whole blood samples is described. the method involves the use of [2h2, 15n]tramadol hydrochloride as an internal standard and chemical ionization with isobutane, employing single-ion monitoring for quantification. it is specific, sensitive and precise, and has high accuracy. the within-run coefficient of variation is about 1% between 25 and 200 ng/ml and 1.8-2.9% at the lowest ...	1985	4019697
[mechanism of the anti-arrhythmic action of phosphocreatine in acute myocardial ischemia].	the association between phosphocreatine's antifibrillatory action and its effect on the excitement propagation processes in the ischemic area was investigated under acute coronary arterial occlusion in dogs. ischemia considerably reduced the amplitude, and increased the duration and time of onset, in local electrograms, and provoked cardiac fibrillation at the time of occlusion or during the recovery of coronary flow. a single intravenous injection of 300 mg/kg phosphocreatine eliminated cardiac ...	1985	4021280
[characteristics of monosynaptic connections between neurons of the callosal system and specific thalamic nuclei].	in the sensorimotor cortex of awake rabbits monosynaptic afferent and efferent connections of callosal neurons (cn) with ipsilateral specific thalamic nuclei (ventral posterolateral, ventral posteromedial, ventral lateral and anteroventral nuclei) were analyzed by electrophysiological method and compared with those of target-units of callosal fibres (tu). it was demonstrated that cn and tu differed in their monosynaptic inputs from the thalamus and in their axons projecting to these structures a ...	1985	4022178
[characteristics of a cell line obtained from a human hypernephroma].	a stable tumorigenic cell line has been obtained from the human hypernephrome. the culture grew as monolayers of large polygonal cells with foamy cytoplasm and 1-2 nucleoli. the cells formed monolayer after the third day after planting into flask and then formed multilayered growth. the electron microscopic studies of hypernephrome sections revealed the dark and light cells. the hypernephrome cells have characteristics of tumor cells. on the 200th passage the cells are aneuploid with a model num ...	1985	4027291
presaccadic 'spike' potential: investigation of topography and source.	approximately 15-30 ms before a saccade, a large-amplitude, relatively short duration potential whose source has not previously been determined with certainty, can be recorded from the scalp in man. we have measured the surface topography of this potential during horizontal saccades in normal subjects and have found it to be maximal near the eye on the side ipsilateral to the direction of the saccade. dipole modelling predicts a source near the eye ipsilateral to the direction of gaze, and the a ...	1985	4027625
presaccadic 'spike' potential: investigation of topography and source.	approximately 15-30 ms before a saccade, a large-amplitude, relatively short duration potential whose source has not previously been determined with certainty, can be recorded from the scalp in man. we have measured the surface topography of this potential during horizontal saccades in normal subjects and have found it to be maximal near the eye on the side ipsilateral to the direction of the saccade. dipole modelling predicts a source near the eye ipsilateral to the direction of gaze, and the a ...	1985	4027625
rapid extraction and purification of diethylstilboestrol in bovine urine hydrolysates using reversed-phase c18 columns before determination by radioimmunoassay.		1985	4030972
systemic complement activation, lung injury, and products of lipid peroxidation.	previously we have demonstrated that systemic activation of the complement system after intravenous injection of cobra venom factor (cvf) results in acute lung injury as reflected by increases in the vascular permeability of the lung as well as by morphologic evidence of damage to lung vascular endothelial cells. in using the vascular permeability of the lung as the reference, the current studies show a quantitative correlation between lung injury and the appearance in plasma of lipid peroxidati ...	1985	4031060
role of uricase in the triggering of germination of bacillus fastidiosus spores.	the likelihood that uric acid was the only compound capable of triggering germination of bacillus fastidiosus spores was reinforced by the finding that ureidoglycollic acid, urea, nh4cl, 2,8-dihydroxypurine and a combination of l-alanine and o-carbamoyl-d-serine were ineffective as germinants. uric acid-triggered germination of b. fastidiosus was prevented by a range of inhibitors that also inhibited uricase activity in dormant spore extracts. o2 uptake during germination started immediately aft ...	1985	4038258
three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. v. assignment of actin in the actin-tropomyosin-myosin subfragment-1 complex.	to assign the actin molecule in the three-dimensional image of the actin-tropomyosin-myosin subfragment-1 (actin-tm-s1) complex, the three-dimensional image of the actin-tropomyosin complex was correlated to that of actin-tm-s1. to assess the similarity of two structures in a quantitative manner, we used a normalized cross-correlation function ("similarity function"). the calculation of similarity indicated that domain a and domain b defined in (1, 2) correspond to actin-tropomyosin. this assign ...	1985	4039721
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.	the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...	1985	4040921
[mechanical injector for angiography].		1985	4051773
determination of zinc in whole blood, plasma and serum using zeeman effect flame atomic absorption spectroscopy.	methods are presented for the determination of zinc in whole blood, plasma and serum using zeeman effect flame atomic absorption spectroscopy and a flame microsampling funnel. whole blood was diluted 1/25 with 0.10 mol/l hydrochloric acid; plasma and serum were diluted 1/5 with deionized water. concentrations could be read directly from standards prepared in human blood pools. the within-run relative standard deviation (rsd) was 0.50%, 0.82% and 0.61% for whole blood specimens with concentration ...	1985	4053384
suppression of human lymphocyte mitogen response by retroviruses of type d. ii. non-activity of mason-pfizer monkey virus versus activity of human cell line derived virus pmfv.	in contrast to the human cell line derived type d retrovirus pmfv, the mason-pfizer monkey virus (mpmv) does not suppress the mitogen response of normal human lymphocytes. both viruses have been propagated on the same cell lines and purified by the same methods. mpmv did not contain a factor able to abolish pmfv-induced suppression of the mitogen response. neither could mpmv suppress the mitogen response of lymphocytes from rhesus monkeys or baboons. pmfv however inhibited their reactivity.	1985	4062558
classically conditioned hyperglycemia in the obese mouse.	the obese (c57bl/6j ob/ob) mouse is a commonly used animal model of non-insulin-dependent diabetes mellitus. it has recently been demonstrated that this mouse is not consistently hyperglycemic, however, unless it is subjected to environmental stress. in the present study, hyperglycemia in obese mice was induced by classical conditioning. obese diabetic mice and lean control animals were exposed to shaking stress. all animals developed hyperglycemia in response to shaking. to demonstrate classica ...	1985	4070525
motor conduction velocity in the human spinal cord: slowed conduction in multiple sclerosis and radiation myelopathy.	transcutaneous electrical stimulation of the central nervous system was used to measure motor conduction velocity in the human spinal cord in 21 subjects aged 22 to 75 years (mean 55 years), none of whom had neurological disease. the motor conduction velocity between the sixth cervical (c6) and first lumbar (l1) vertebral levels was 67.4 +/- 9.1 m/s. this probably represents conduction velocity in the corticospinal tracts. in these subjects the motor conduction velocity in the cauda equina, betw ...	1985	4078579
alu sequences transcription in x. laevis oocytes: nuclear-cytoplasmic partitioning and evidence for 3' end processing reactions.	a large alu-family cluster in the 5' flanking region of a human alpha 1-acid glycoprotein gene has been identified and sequenced. individual members microinjected into x. laevis oocytes are transcribed only when canonical box a and b components of the split pol iii promoter are present. alu transcripts accumulate in the nucleus. an unusually short alu transcript, able to assume a stable secondary structure, undergoes a 3' end processing reaction similar to the one required for trna 3' end matura ...	1985	4080545
inhibition of histamine-n-methyltransferase activity by neuromuscular blocking drugs.	on the basis of previous findings that histamine-n-methyltransferase (hmt) activity can be significantly enhanced or inhibited by a number of analogues of histamine and drugs containing dialkylaminoalkyl moieties, we investigated whether the neuromuscular blocking drugs alcuronium, d-tubocurarine, decamethonium, succinylcholine, gallamine and pancuronium each of which contain quaternary ammonium groups, influence the activity of hmt. our findings showed that all six drugs significantly inhibited ...	1985	4083176
fourteen years of shigellosis in dhaka: an epidemiological analysis.	we examined whether the proportion of shigellae patients among diarrhoeal cases, the distribution, species, case-fatality rates and hospital visits changed over time in dhaka. we isolated 19639 shigella strains from 822812 diarrhoea cases treated at the international centre for diarrhoeal disease research, bangladesh (icddr,b), between 1969 and 1982. the number of cases increased from 209 (2.5%) in 1969 to 4833 (7.7%) in 1976. extrapolating from a 4% vigorous systematic sample of icddr,b hospita ...	1985	4086147
[ultrastructural aspects of the wound healing process of the skin of albino rats].		1985	4089424
amino acid and protein metabolism in dorsal root ganglia of rabbits with experimental allergic neuritis.	amino acid and protein metabolism has been studied in the dorsal root ganglia of rabbits with experimental allergic neuritis (ean). the concentrations of a number of nonessential amino acids (glutamine, serine, aspartate, and glutamate) were reduced in the spinal ganglia of ean animals without any comparable change in the blood plasma. the short-term influx of glycine and gaba was decreased in ean animals, whereas that of histidine and valine was not altered. the prolonged accumulation of all th ...	1985	4094728
[effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats].		1985	3835674
4-hydroxyalk-2-enals are substrates for glutathione transferase.	the 4-hydroxyalk-2-enals are established products of lipid peroxidation that are conjugated with intracellular glutathione. cytosolic glutathione transferases from rat liver were shown to give high specific activities with 4-hydroxynonenal and 4-hydroxydecenal. the isoenzyme giving the highest specific activity was glutathione transferase 4-4. the rate of the spontaneous conjugation reaction is negligible in comparison with the rate calculated for the cellular concentration of the glutathione tr ...	1985	3838159
shigella toxin and the pathogenesis of shigellosis.	shigella dysenteriae 1 produces a periplasmic protein with multiple toxic effects in vivo and in vitro. these include neurotoxicity, cytotoxicity and enterotoxicity, as well as the ability to inhibit cell-free protein synthesis. the purified toxin is a protein of relative molecular mass (mr) 64 000. it is composed of one catalytically active a subunit (mr = 32 000) that inhibits protein synthesis, and a complex of five b monomers (mr approximately 6500 each). studies using subunit-specific antib ...	1985	3847336
theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: xiv. mutational and non-mutational mechanisms as subsets of a more general mechanism. part c. a defined cancer mutation.	the results of applying a mechanism of ethionine-induced embryonic gene derepressions to explain similar features found in hereditary tyrosinemia have been extended to a well defined cancer mutation. in all three cases, the described mechanism is compatible with the explanation for the etiology of embryonic like phenotypic expressions in cells and potentially for the carcinogenic process. the essence of the formulated process for a human bladder carcinoma mutation in the ras gene for a protein p ...	1985	3853079
protein loss of bovine dental enamel during in-vitro subsurface demineralization.	a chemical system based on the dialysis principle was used to study protein loss of dental enamel during demineralization with an acetic-acid buffer solution containing calcium and phosphate, in which the fluoride-ion activity was kept constant. this resulted in a subsurface lesion, with a depth of about 130 microns. after demineralization, protein material was isolated from the demineralization solution. u.v. spectra of the protein showed strong absorbance between 240 and 300 nm. amino-acid com ...	1985	3865639
[the development of the human dentition. iv].		1985	3867519
[the development of the human dentition. iv].		1985	3867519
[different clinical and prognostic aspects of angina pectoris in unstable phase].	the purpose of this study was to focus on the clinical and angiographic characteristics of 113 patients with crescendo angina (group i) as compared to 187 patients with angina of new onset (group ii), selected from a series of 474 consecutive subjects, admitted to our clinic between january 1976 and july 1983 because of recurrent episodes of spontaneous angina, who underwent cardiac catheterization and coronary angiography within one month of hospitalization. group i patients showed a greater in ...	1985	3878310
shiga-like cytotoxin production by enteropathogenic escherichia coli serogroups.	the mechanism by which enteropathogenic escherichia coli (epec) cause disease remains to be defined. we studied epec and non-epec strains of e. coli from stool specimens obtained from infants and adults for production of shiga-like cytotoxin. although it was common for healthy infants and adults to have cytotoxin-producing e. coli as part of the fecal flora, shiga-like cytotoxin was detected more commonly and in greater amounts among epec than among other fecal e. coli. these results suggest a r ...	1985	3880727
brain oedema following brain ischaemia and the influence of therapy.		1985	3881111
addition of three new serotypes of shigella boydii to the shigella schema.	no new serotypes have been added to the shigella schema since 1958, although several provisional serotypes have been described. we conducted biochemical and serological studies on three provisional shigella boydii serotypes. four strains of serotype 2710-54 from four widely separated countries, 7 strains of serotype 3615-53 from three different countries, and 31 strains of serotype 1344-78 (e10163) from six different countries were included. reactions of all three serotypes were consistent with ...	1985	3881469
an animal model of haemolytic--uraemic syndrome in shigellosis: lipopolysaccharides of shigella dysenteriae i and s. flexneri produce leucocyte-mediated renal cortical necrosis in rabbits.	to develop an animal model of the haemolytic-uraemic syndrome during shigellosis, rabbits were injected with lipopolysaccharides (lps) extracted by the hot phenol-water method from shigella dysenteriae i and from s. flexneri. two intravenous injections of lps spaced by 24 h elicited renal cortical necrosis in a generalized shwartzman reaction characterized by fibrin deposition in glomerular capillaries and by elevated plasma creatinine concentration. rabbits rendered leucopenic by busulphan trea ...	1985	3882120
a new provisional serovar of shigella dysenteriae.	bacterial isolates obtained in israel from stool cultures of 17 sporadic cases of acute diarrhea during the years 1972 to 1980 and from 14 patients involved in an institutional outbreak in 1984 are described. these cultures gave the biochemical reactions and pathogenicity tests characteristics of the genus shigella but failed to agglutinate, living or boiled, in any of the recognized or provisional shigella antisera. all 31 cultures were biochemically and serologically identical, and owing to th ...	1985	3882749
plasmid characterization of drug-resistant shigella dysenteriae 1 from an epidemic in central africa.	a widespread epidemic of severe dysentery in zaire and neighbouring central african countries was caused by a multiply drug-resistant strain of shigella dysenteriae 1. early isolations were resistant to ampicillin, chloramphenicol, streptomycin, sulphonamides and tetracyclines (r-type = acssut). later in the epidemic strains resistant to trimethoprim (tm) became prevalent and a few strains resistant to kanamycin (k) or nalidixic acid were also isolated. all resistances except nalidixic acid were ...	1985	3886782
shiga toxin--an expanding role in the pathogenesis of infectious diseases.		1985	3886803
[role of the conjugative transfer of r-plasmids in competitive interactions between plasmid-containing and plasmid-free strains of escherichia coli in continuous culture].	competition of the plasmid-containing strain c600 (rp4) (pbs94) of e. coli and the plasmid-free strain c600 rifr of e. coli in chemostatic and ph-static continuous cultures resulted in displacement of the plasmid-free strain in spite of its higher specific growth rate. conjugative transfer of rp4 and pbs94 plasmids into the cells of the plasmid-free strain was observed in the experiments. competition of strains c600 (pbs94) (rp4 tra-) and c600 rifr in the chemostatic culture in the absence of th ...	1985	3888095
properties of rat liver signal peptidase reconstituted into liposomes.	edta/kcl- or pyrophosphate-treated rough microsomes of rat liver clearly showed the co-translational cleavage of pre-human placental lactogen and translocation of the product into membrane vesicles. the signal peptidase fraction was isolated by chromatography on sephacryl s-300 of deoxycholate-treated membranes and reconstituted into liposomes by dialysis or by the biobeads sm-2 method. assay of the signal peptidase activity was performed with pre-human placental lactogen synthesized by the reti ...	1985	3888971
treatment of severe bacillary dysentery with trimethoprim alone.	trimethoprim (300 mg twice daily for five days) and co-trimoxazole (two tablets twice daily for five days) were compared as treatment for adult patients with severe shigellosis in rwanda. excellent bacteriological and clinical results were obtained with both regimens, with the exception of patients infected with a trimethoprim-resistant strain of shigella dysenteriae type 1. since only 20 patients were investigated, the conclusions of our study do not reach statistical significance. before recom ...	1985	3890282
an epidemic of bacillary dysentery at raipur.		1985	3891837
enzyme immunoassay of human protein c by using monoclonal antibodies.	an enzyme-linked immunosorbent assay (elisa) for measuring human protein c by using two monoclonal antibodies directed toward the heavy chain of protein c is reported. this assay enabled the determination of protein c in concentrations of 10 to 400 ng/ml in less than 3 hours with a single antigen-antibody reaction. within-run and between-run coefficients of variation were less than 8%. the mean concentrations of protein c in plasma of 42 normal subjects, 24 patients with liver disease, 27 with d ...	1985	3895562
transformation of trypomastigote forms of trypanosoma cruzi into activators of alternative complement pathway by immune igg fragments.	in this report we examined the capacity of immune igg fragments to prepare trypomastigote bloodstream forms (tbf) of trypanosoma cruzi for lysis. f(ab')2 fragments were capable of presensitizing tbf for complement (c) lysis, thus excluding the participation of fc domains in the c activation process. an intact hinge region of the igg molecule was not involved either, since the corresponding fab' were almost as active as the original molecules in preparing tbf for lysis. fab also retained such act ...	1985	3898353
transformation of trypomastigote forms of trypanosoma cruzi into activators of alternative complement pathway by immune igg fragments.	in this report we examined the capacity of immune igg fragments to prepare trypomastigote bloodstream forms (tbf) of trypanosoma cruzi for lysis. f(ab')2 fragments were capable of presensitizing tbf for complement (c) lysis, thus excluding the participation of fc domains in the c activation process. an intact hinge region of the igg molecule was not involved either, since the corresponding fab' were almost as active as the original molecules in preparing tbf for lysis. fab also retained such act ...	1985	3898353
plasmid-mediated drug resistance of shigellae in kuwait.	of 153 clinical isolates of shigellae examined, 64.7% belonged to shigella flexneri, 18.9% to sh. sonnei, 11.8% to sh. boydii and 4.6% to sh. dysenteriae. part of these isolates were resistant to sulfamethoxazole and streptomycin (88.2% each), ampicillin (66.7%), tetracycline (63.4%) and co-trimoxazole (43.1%), with levels of resistance (mic50 and mic90) being invariably high. resistance to three or more drugs (multidrug resistance) was seen in 77.8% of the isolates. all the 25 strains examined ...	1985	3899007
shigellosis (antibiotic resistance and transfer of r-factor).		1985	3900357
characterization of monoclonal antibodies against shiga-like toxin from escherichia coli.	three monoclonal antibodies, designated mab 16e6, mab 13c4, and mab 19g8, were produced which recognize shiga-like toxin (slt) from escherichia coli. all three monoclonal antibodies neutralized the cytotoxicity of e. coli slt and were able to immunoprecipitate intact labeled toxin with staphylococcus aureus protein a. the three antibodies were of the g1 heavy and kappa light chain classes. mab 16e6 bound to the b subunit of slt in western blots and also neutralized the lethality of the toxin for ...	1985	3905611
changing pattern of resistant shiga bacillus (shigella dysenteriae type 1) and shigella flexneri in bangladesh.	shigella dysenteriae type 1 (shiga bacillus) has made a dramatic comeback in bangladesh after 10 years when shigella flexneri was the dominant serogroup. whereas s. flexneri showed little increase in resistance to the commonly used antimicrobial agents--namely, trimethoprim-sulfamethoxazole (tmp-smx) and ampicillin-over the years, s. dysenteriae type 1 underwent rapid changes with regard to sensitivity pattern during the last two years. the first tmp-smx-resistant strain of s. dysenteriae type 1 ...	1985	3905980
the changing pattern of resistance to ampicillin and co-trimoxazole in shigella serotypes in bangalore, southern india.		1985	3905991
[3-year study of shigellosis epidemic in rwanda, central africa. problems of public health and bacteriological aspects].	since 1979, the lakes region (kivu in zaïre, western uganda, rwanda, burundi) has been stricken by a bacillary dysentery epidemic at s. dysenteriae type 1. supposed to be extinct since the 1950s., this bacillus has revealed a very noticeable virulence as well by the number of registered cases (524/100.000 in 1983 at ruhengeri-rwanda) as its fast break through. demographic density (from 400 to 600/km2) has played an important role and has facilitated the transition toward epidemicity. systematic ...	1985	3906345
determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection.		1985	3907412
outbreak of shigella dysenteriae type-1 dysentery in himachal pradesh--aetiologic investigation in distt: shimla.		1985	3908554
[the use of an enzymatic preparation called alcalase 60 l].		1985	3910192
mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12.	the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ...	1985	3911022
plasmid characterization of shigella spp. isolated from children with shigellosis and asymptomatic excretors.	strains of shigella dysenteriae type 1 and sh. flexneri isolated during 1981-82 from children with shigellosis and also from asymptomatic excretors were examined for drug resistance and for the presence of plasmids. shigella strains isolated from dysenteric children showed multiple plasmid bands. thirty-eight percent of the strains transferred the drug resistance factor(s), either partially or fully to a recipient escherichia coli k12 strain. plasmids in the molecular weight range from between 4 ...	1985	3912364
[epidemiological analysis of the outbreak of bacillary dysentery in xinjiang].		1985	3915444
pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells.	pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ...	1985	3925713
[virulence of shigella: epithelial cell invasiveness and other dysentery-producing factors].		1985	3938489
synaptic transmission in the rabbit inferior mesenteric ganglion.	electrical properties, cholinergic neurotransmission and non-cholinergic neurotransmission in the rabbit inferior mesenteric ganglion (img) in vitro were examined with intracellular recording techniques. a single ganglionic neuron received an average of 42 nicotinic cholinergic synaptic inputs. an atropine-sensitive slow excitatory postsynaptic potential not followed by a non-cholinergic late slow excitatory postsynaptic potential (ls-epsp) was observed in 7% of the cells. in 63% of the cells a ...	1985	3003179
interaction of stable prostaglandin endoperoxide analogs u46619 and u44069 with human platelet membranes: coupling of receptors with high-affinity gtpase and adenylate cyclase.	it has been demonstrated using a membrane preparation of human platelets that stable analogs of pgh2, u46619 and u44069, control the activity of adenylate cyclase and a high-affinity hormone-sensitive gtpase. at 10(-8)-10(-6) m, the analogs inhibit the basal activity of adenylate cyclase by 20-25%. with a further rise in u46619 and u44069 concentrations up to 10(-5)-10(-4) m, the inhibition is abolished and adenylate cyclase activity is stimulated in a dose-dependent fashion. in the presence of ...	1985	3003958
[experimental basis for the use of collagenase preparations to prevent aging of the skin].		1985	3004061
near infrared optical monitoring of intact skeletal muscle during hypoxia and hemorrhagic hypotension in cats.		1985	3008518
middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src.	we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ...	1985	2981329
serologic survey of rotavirus, norwalk agent and prototheca wickerhamii in wastewater workers.	analysis of paired sera from 48 wastewater workers and controls who reported gastrointestinal illness did not reveal any excess of seroconversions to norwalk agent or to rotavirus. inexperienced wastewater-exposed workers had higher levels of antibody to norwalk agent than did experienced and control workers and those with high and medium aerosol exposure had higher titres than those in the low aerosol category. analysis for prototheca antibody titres was essentially negative.	1985	2981485
immunologic characterization of herpes simplex virus type 2 antigens icp10 and icsp11/12.	infected cell protein 10 (icp10) or antigen 4 (ag4) and infected cell-specific protein 11/12 (icsp11/12) have been suggested as specific antigenic markers for cervical carcinoma. experiments were designed to determine whether icp10 and icsp11/12 are distinct antigens and to determine the cellular localization of icp10. results indicate that an apparent 160 kdalton (kda) protein analyzed by 8.5% polyacrylamide gels (= 144 kda protein analyzed by 7.0% polyacrylamide gels) was detected in hsv-2-inf ...	1985	2988230
induction of cellular dna synthesis by a simian virus 40 mutant defective in nuclear transport of t antigen.	the simian virus 40 (sv40) (ct)-3 mutant [sv40(ct)-3], which is defective in nuclear transport of t antigen, was utilized to determine whether cellular dna synthesis can be stimulated by sv40 in the absence of detectable nuclear t antigen. cellular dna synthesis was examined in the temperature-sensitive cell cycle mutants, bhk ts13 and bhk tsaf8, after microinjection of quiescent cells with plasmid dna containing cloned copies of wild-type sv40 or sv40(ct)-3. the efficiency of induction of cellu ...	1985	2993869
the biologic significance of the mixed lymphocyte kidney culture in humans.	the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...	1985	2996186
elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist.	the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ...	1985	2999844
elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist.	the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ...	1985	2999844
court of appeals rules dhss notice on family planning contrary to law.	in the december 1982 case of gillick v. west norfolk and wisbech aha, the british court of appeal acceded to mrs. victoria gillick's petition that no professional employed by the health authority be permitted to give contraceptive or abortion treatment to any of her children under the age of 16 without her prior knowledge and consent. the ruling held that a 1980 department of health and social security notice allowing treatment of minors without parental consent was contrary to law. the court ...	1985	2856993
nalidixic-acid-resistant shigella dysenteriae i.		1985	2858035