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synthesis and anti-microbial screening of some schiff bases of 3-amino-6,8-dibromo-2-phenylquinazolin-4(3h)-ones.in the present study, a series of novel schiff bases were synthesized by condensation of 3-amino-6,8-dibromo-2-phenylquinazolin-4(3h)-ones with different aromatic aldehydes via cyclized intermediate 6,8-dibromo-2-phenyl benzoxazin-4-one. the chemical structures were confirmed by means of ir, (1)h nmr, (13)c nmr, mass spectral and elemental analysis. these compounds were screened for anti-bacterial (staphylococcus aureus atcc-9144, staphylococcus epidermidis atcc-155, micrococcus luteus atcc-4698 ...200918603337
microbiological assessment of cadaver skin grafts received in a skin bank.study on the microbiological assessment of cadaver skin grafts was undertaken in the skin bank at the department of surgery in l.t.m. medical college and hospital, mumbai, india.200918603376
design, synthesis and antibacterial activity of novel actinonin derivatives containing benzimidazole heterocycles.a series of novel actinonin derivatives containing a benzimidazole heterocycle linked as amide isostere have been designed and synthesized. the structures of all the synthesized compounds were confirmed by analytical and spectroscopic methods. all the compounds were evaluated in vitro against staphylococcus aureus, klebsiella pneumoniae, and sarcina lutea. among them, compound 1a with unsubstituted benzimidazole ring exhibited potent antibacterial activities.200918617291
mitracarpus frigidus aerial parts exhibited potent antimicrobial, antileishmanial, and antioxidant effects.the crude extract and the hexane, ch(2)cl(2), etoac, n-buoh, and hydromethanolic fractions of the aerial parts of mitracarpus frigidus were evaluated against promastigote forms of two species of leishmania (l. chagasi and l. amazonensis), 11 strains of bacteria (staphylococcus aureus, pseudomonas aeruginosa, salmonella enterica sorovar tythimurium, shigella sonnei, klebsiella pneumoniae, escherichia coli, micrococcus luteus, enterococcus faecalis, enterobacter cloacae, streptococcus pyogenes and ...200918644325
effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by klebsiella pneumoniae under micro-aerobic conditions.glycerol dehydrogenase (gdh) and 1,3-propanediol (1,3-pd) oxidoreductase had been proved two key enzymes for 1,3-pd production by klebsiella pneumoniae. fed-batch fermentations of the recombinant k. pneumoniae strains, over-expressing the two enzymes individually, were carried out under micro-aerobic conditions, and the behaviors of the recombinants were investigated. results showed that over-expression of 1,3-pd oxidoreductase did not affect the concentration of 1,3-pd. however, it enhanced the ...200918682988
risk factor analysis of invasive liver abscess caused by the k1 serotype klebsiella pneumoniae.the increasing prevalence of klebsiella pneumoniae liver abscess in asian countries is attributable to virulent strains of the k1 serotype. we investigated the risk factors for the k1 serotype k. pneumoniae liver abscess. a case-control study was performed using the database of a nationwide study of liver abscess in korea. multivariate logistic regression analysis was performed for 78 cases of the k1 serotype k. pneumoniae liver abscess and 81 controls with non-klebsiella. diabetes mellitus was ...200918663497
gene cloning, expression, and characterization of a novel phytase from dickeya paradisiaca.a novel phytase gene, appa, was isolated by degenerate polymerase chain reaction (pcr) and thermal asymmetric interlaced pcr from dickeya paradisiaca. the full-length appa comprises 1278 bp and encodes 425 amino acid residues, including a 23-residue putative n-terminal signal peptide. the deduced amino acid sequence of appa reveals the conserved motifs rhgxrxp and hd, which are typical of histidine acid phosphatases; significantly, appa shows maximum identity (49%) to a phytase from klebsiella p ...200918679591
proteolytic activity in stored aerobic granular sludge and structural integrity.aerobic granules lose stability during storage. the goal of this work was to highlight the main cause of stability loss for stored granules as intracellular protein hydrolysis. the quantity of extracellular proteins was noted to be significantly lower during granule storage, and protease enzyme activities were correspondingly higher in the cores of stored granules. the proteolytic bacteria, which secrete highly active protease enzymes, were for the first time isolated and characterized by analyz ...200918614356
causative agents and antimicrobial susceptibilities of urinary tract infections in the northwest of iran.the empirical therapy of urinary tract infections (uti) relies on the predictability of the agents causing uti and knowledge of their antimicrobial susceptibility patterns.200918703368
antimicrobial resistance patterns of urinary tract pathogens and rationale for empirical therapy in turkish children for the years 2000-2006.in patients with suspected urinary tract infection (uti), antibiotic treatment is usually started empirically, before urine culture results are available. unfortunately, antibiotic resistance has become an increasingly pressing clinical issue in many countries. the objective of this study was to assess the changing susceptibility of urinary pathogens to commonly used antimicrobials in a six-year period to evaluate the options for empirical antibiotic therapy in children with community acquired u ...200918704743
nationwide surveillance of antimicrobial resistance among enterobacteriaceae in intensive care units in taiwan.to determine the antimicrobial resistance profiles among clinical isolates of enterobacteriaceae in taiwanese intensive care units (icus), a national surveillance of antibiotic resistance among important enterobacteriaceae was conducted from september 2005 through november 2005 at the icus of ten major teaching hospitals in taiwan. a total of 574 enterobacteriaceae isolates recovered from various clinical samples of our icu patients were submitted for in vitro test. minimum inhibitory concentrat ...200918716805
susceptibility of common aerobic pathogens to tigecycline: results of a surveillance study in germany.tigecycline is a novel antimicrobial agent for parenteral use encompassing a broad spectrum of bacterial pathogens, including multi-resistant organisms. here, we report the results of the first nationwide surveillance trial that was conducted in order to evaluate the susceptibility of bacterial isolates to tigecycline in a european country prior to its clinical use. a total of 2,610 gram-positive and gram-negative organisms recovered from hospitalized patients were tested. minimum inhibitory con ...200918716808
rapid detection of bla(kpc)-positive klebsiella pneumoniae in a clinical setting. 200918719955
effect of electrical current on the activities of antimicrobial agents against pseudomonas aeruginosa, staphylococcus aureus, and staphylococcus epidermidis biofilms.bacterial biofilms are resistant to conventional antimicrobial agents. prior in vitro studies have shown that electrical current (ec) enhances the activities of aminoglycosides, quinolones, and oxytetracycline against pseudomonas aeruginosa, klebsiella pneumoniae, staphylococcus epidermidis, escherichia coli, and streptococcus gordonii. this phenomenon, known as the bioelectric effect, has been only partially defined. the purpose of this work was to study the in vitro bioelectric effect on the a ...200918725436
synthesis, characterization and antimicrobial activity of co(ii), zn(ii) and cd(ii) complexes with n-benzyloxycarbonyl-s-phenylalanine.in this paper the first complexes of m(2+) ions (m(2+) = zn(2+), cd(2+) and co(2+)) with n-benzyloxycarbonyl-s-phenylalaninato ligand (1-3) are described. the new complexes were characterized by means of elemental analysis, ir and uv-vis spectroscopy, molar conductivity measurements and (1)h, (13)c and (15)n nmr spectroscopy (1d and 2d). the co(ii) complex adopts an octahedral geometry, and the zn(ii) and cd(ii) complexes adopt a tetrahedral one. for the first time, the antimicrobial activity of ...200918760509
synthesis, structure and biological activity of copper(ii) complexes of 4-(2-pyridylmethyl)-1,7-dimethyl-1,4,7-triazonane-2,6-dione and 4-(2-pyridylethyl)-1,7-dimethyl-1,4,7-triazonane-2,6-dione.four mononuclear copper(ii) complexes 1-4 have been synthesized with two new n-functionalized macrocyclic ligands l(1) and l(2). all complexes are well characterized by various spectroscopic techniques, elemental analyses and conductivity measurements. results suggest that cu(ii) ion has n(2)o coordination from ligand and s(2) from two coordinated solvent molecules (sch(3)cn for 1 and 3 while ch(3)oh for 2 and 4). the crystal structure of a representative complex 3 strengthen the proposed formul ...200918775587
infections in a surgical intensive care unit of a university hospital in greece.we aimed to evaluate the clinical and microbiological characteristics of the patients who developed an infection in our surgical intensive care unit (sicu).200918775663
chemical composition and antibacterial activity of the essential oil of monticalia andicola (asteraceae) collected in venezuela.the essential oil from the leaves of monticalia andicola turcz., collected in november 2008, was analyzed by gc/ms. a yield of 0.15% oil was obtained by hydrodistillation. thirty-six components were identified by comparison of their mass spectra with those in the wiley gc-ms library data base. the major components were alpha-pinene (19.6%), beta-pinene (10.5%), alpha-longipinene (6.5%), delta-3-carene (6.2%), cyperene (5.4%) and beta-phellandrene (5.2%). the antibacterial activity of the essenti ...200919967999
in vitro synergistic effect of doxycycline & ofloxacin in combination with ethanolic leaf extract of vangueria spinosa against four pathogenic bacteria.the in vitro antibacterial activity of ethanolic leaf extract of vangueria spinosa roxb. (rubiaceae) alone and in combination with antibiotics (doxycycline and ofloxacin) by means of fractional inhibitory concentration indices (fici) as well as by the use of time-kill assays against one gram-positive bacterium (staphylococcus aureus) and three gram-negative bacteria (escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa) was studied.200919942754
microbial origin of phenylcarboxylic acids in the human body.in previous studies we demonstrated increased amounts of phenylcarboxylic acids (pca) in serum of patients with sepsis. this observation prompted the present study of the ability of the human microbiome bacteria to produce pca in vitro. pca were detected in culture media by gas chromatography-mass spectrometry. increased amounts of phenyllactic and p-hydroxyphenyllactic acids were produced by klebsiella pneumonia, escherichia coli, and staphylococcus aureus. certain strict anaerobes (bifidobacte ...200919961416
symbiotic nitrogen fixation in the fungus gardens of leaf-cutter ants.bacteria-mediated acquisition of atmospheric n2 serves as a critical source of nitrogen in terrestrial ecosystems. here we reveal that symbiotic nitrogen fixation facilitates the cultivation of specialized fungal crops by leaf-cutter ants. by using acetylene reduction and stable isotope experiments, we demonstrated that n2 fixation occurred in the fungus gardens of eight leaf-cutter ant species and, further, that this fixed nitrogen was incorporated into ant biomass. symbiotic n2-fixing bacteria ...200919965433
[enteropathogens relating to diarrhea in hiv patients on antiretroviral therapy].the etiology of the diarrheic process in aids may be caused by viruses, bacteria, fungi, protozoa or helminths, as well as hiv itself. this study evaluated enteropathogens relating to diarrhea in hiv patients who were on antiretroviral therapy. the parasitological methods used were faust, hoffmann and kinyoun. isolation and culturing of fungi were carried out in accordance with the methodology recommended by the nccls m27-a standard. the yeast species were identified using the polymerase chain r ...200919967238
[prevalence of the tem, shv and ctx-m families of extended-spectrum beta-lactamases in escherichia coli and klebsiella spp at the university hospital of santa maria, state of rio grande do sul].in this study, the distribution and prevalence of extended-spectrum beta-lactamases belonging to the tem, shv and ctx-m families were estimated among samples of escherichia coli and klebsiella spp. at the university hospital of santa maria, rio grande do sul. over a 14-month period, 90 microorganisms were selected as likely esbl producers. the isolates were subjected to confirmatory phenotype tests for the presence of esbl. through investigating the respective genes using the polymerase chain re ...200919967239
[extension of beta-lactamases producing bacteria is a worldwide concern].since 2000's, community extended-spectrum beta-lactamases (esbl) producing bacteria have spread worldwide, i.e. mostly escherichia coli that produce esbl such as ctx-m enzymes. previous cephalosporins and fluoroquinolones usage are the two most frequent risk factors identified in patients that harbor esbl-producing bacteria. in addition surveys have shown an alarming trend of associated resistance to others classes of antimicrobial agents among isolates. the emergence of esbl-producing isolates ...200919951668
[lung abscess due to community acquired klebsiella pneumonia].in western countries, community-acquired pneumonias due to klebsiella pneumoniae (kp) are rare and associated with a poor prognosis and a high mortality. the severity is in part linked to the virulence of kp. immuno-depression, sepsis and visceral abscesses are frequently found, constituting other classical risk factors for severity and contributing to the poor prognosis. the therapeutic strategy is based on third generation cephalosporins, aminoglycosides and quinolones.200919953020
characterization of stable fly (diptera: muscidae) larval developmental habitat at round hay bale feeding sites.in this study, we examined the stable fly, stomoxys calcitrans (l.) (diptera: muscidae), larval developmental habitat within the round hay bale feeding sites on cattle pastures, and we identified three zones with distinct characteristics around two types of hay feeders (ring and cone). the parameters monitored in each zone included stable fly emergence, substrate temperature, moisture, ph, thickness of hay-manure layer, and concentration of fecal coliform bacteria (escherichia coli and klebsiell ...200919960675
antimicrobial properties of the stem bark of saraca indica (caesalpiniaceae).chloroform, methanol, aqueous and ethanolic extracts of the stem bark of saraca indica were investigated for their antibacterial and antifungal activity against standard strains of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus cereus, klebsiella pneumoniae, proteus mirabilis, salmonella typhimurium and streptococcus pneumoniae and the fungi: candida albicans and cryptococcus albidus. methanolic and aqueous extract exhibited antimicrobial activity with mic ranging from ...200919961043
extended-spectrum beta-lactamase-producing phenotype signifies a poor prognosis for patients with cefpodoxime-resistant escherichia coli or klebsiella pneumoniae bacteremia.bloodstream infections caused by multidrug-resistant enterobacteriaceae are a major concern. this study explored the clinical impact of extended-spectrum beta-lactamase (esbl) production among cefpodoxime-resistant escherichia coli and klebsiella pneumoniae bacteremia.200919949753
in vitro susceptibilities of aerobic and facultative anaerobic gram-negative bacilli isolated from patients with intra-abdominal infections at a medical center in taiwan: results of the study for monitoring antimicrobial resistance trends (smart) 2002-2006.the study for monitoring antimicrobial resistance trends (smart) was initiated to monitor the in vitro antimicrobial susceptibility of aerobic and facultative anaerobic gram-negative bacilli (gnb) isolated from patients with intra-abdominal infections (iai). this report summarizes the smart data from 1 of the study centers from 2002 to 2006.200919949755
role of yiax2 in l-ascorbate transport in klebsiella pneumoniae 13882.the yiak-s operon is required for aerobic growth on l-ascorbate in several enterobacteriaceae. here we present evidence that the yiax2 gene belonging to the yiak-s operon of klebsiella pneumoniae 13882, which encodes a protein similar to the putative transporters classified as the major facilitator superfamily, is involved in the uptake of l-ascorbate. concentration kinetic analysis yielded an apparent k(m) of yiax2 for l-ascorbate of 161.38 +/-8.28 micromol x l(-1) and a vmax of 3.81 +/- 0.60 n ...200919940941
[a case of empyema due to capnocytophaga sp. and actinomyces israelii].a 54-year-old man was admitted to our hospital for investigation of cough, sputum production, and fever of 1 month's duration. his diabetes mellitus was poorly controlled, and his hemoglobin hba1c value was elevated at 10.9%. chest x-ray film and computed tomography scan showed bilateral but predominantly right-sided pleural effusion. aspiration of the pleural fluid from the right-side showed frank pus, and empyema was diagnosed. capnocytophaga sp. and actinomyces israelii were isolated in the p ...200919882914
[antibacterial activity of quinolones against various clinically isolated strains and evaluation of efficacy based on the pharmacokinetics/pharmacodynamics theory].we compared the antimicrobial activities of oral quinolones, ciprofloxacin (cpfx), gatifloxacin (gflx), garenoxacin (grnx), levofloxacin (lvfx), moxifloxacin (mflx), norfloxacin (nflx), prulifloxacin (pufx), and tosufloxacin (tflx) using streptococcus pneumoniae, haemophilus influenzae, streptococcus agalactiae, streptococcus pyogenes, extended spectrum beta-lactamase(esbl) producing klebsiella pneumoniae, and methicillin-susceptible staphylococcus aureus (mssa) isolated from clinical materials. ...200919882980
[analysis on the sensitivity to beta-lactam antibiotics of respiratory-infectious isolates on the second survey on the sensitivity of isolates conducted by the japanese society of chemotherapy in 2007--concerning the aspect of pk/pd break points].sensitivity to beta-lactam antibiotics of isolates clinically obtained from respiratory infection sites in adults on the second survey on sensitivity of isolates conducted by the japanese society of chemotherapy in 2007 was investigated according to the classification of the "guideline for treatment for adult nosocomial pneumonia in 2008". among the primary antibacterial drugs for mild (a) and moderate (b) nosocomial pneumonia in adults, beta-lactam antibiotics; ceftriaxone (ctrx), sulbactam/amp ...200919882981
spontaneous bacterial peritonitis: a severe complication of liver cirrhosis.this report presents a survey of current knowledge concerning one of the relatively frequent and severe complications of liver cirrhosis and associated ascites-spontaneous bacterial peritonitis. epidemiology, aetiology, pathogenesis, clinical manifestation, diagnosis and present possibilities of treatment are discussed.200919938187
multidetector row ct diagnosis of an infected right atrial thrombus following repeated dialysis catheter placement.right atrial thrombus formation is a known complication of dialysis catheter placements. we describe the case of a 61-year-old woman with end-stage renal failure who presented with gram-negative septicaemia. a gas-containing filling defect was noted incidentally in the right atrium during a ct scan of the abdomen and pelvis, indicative of a thrombus infected by a gas-forming organism. the finding correlated with a positive blood culture of klebsiella pneumoniae and the two-dimensional echocardio ...200919934063
[infectious endocarditis secondary to an infrequent agent in a patient with haemodialysis]. 200919936011
extended spectrum beta-lactamases among gram-negative bacterial isolates from clinical specimens in three major hospitals in northern jordan.background and objectives. extended spectrum beta-lactamase (esbl) production is increasing all over the world, and organisms other than e. coli and k. pneumoniae are acquiring this character. esbl production is detectable by automation, e-test, double disk diffusion (ddd), and pcr. this study aimed to determine the prevalence of esbl production among clinical isolates of gram-negative rods, and to evaluate the effectiveness of augmentation of clavunate with cefotaxime, ceftazoxime, aztreonam, c ...200919936109
bacteraemia and candidaemia: a considerable and underestimated complication of severe rotavirus gastroenteritis.despite the high incidence of rotavirus gastroenteritis, secondary bacteraemia later in the course of the disease has rarely been reported. to date, the exact incidence of this complication has not been determined. a prospective study was conducted between january 2007 and december 2008 to determine the incidence of bacteraemia by organisms of the normal intestinal flora during severe rotavirus gastroenteritis. rotavirus gastroenteritis was diagnosed by antigen detection in stool. a previously d ...200919922069
automatic implantable cardioverter defibrillator pocket infection due to providencia rettgeri: a case report.coagulase-negative staphylococci and staphylococcus aureus are the commonest pathogens involved in infections of pacemaker-defibrillator systems. among causative gram-negative bacteria, infections due to klebsiella, serratia, pseudomonas, acinetobacter and other species have been reported. we report herein a unique case of an automatic implantable cardioverter defibrillator infection due to providencia rettgeri in a 65-year-old male who was admitted to our service with bacteremia and infection o ...200919918391
marked hydronephrosis and hydroureter after distigmine therapy in an adult male patient with paraplegia due to spinal cord injury: a case report.distigmine, a long-acting anti-cholinesterase, is associated with side effects such as parkinsonism, cholinergic crisis, and rhabdomyolysis. we report a spinal cord injury patient, who developed marked hydronephrosis and hydroureter after distigmine therapy, which led to a series of complications over subsequent years.200919918519
hospital and community isolates of uropathogens at a tertiary hospital in south africa.to investigate the profile of common uropathogens isolated from urine specimens submitted to the diagnostic microbiology laboratory at a tertiary teaching hospital and assess their antimicrobial susceptibility patterns to commonly used antimicrobial agents.200919908617
severe community-acquired acinetobacter baumannii pneumonia: an emerging highly lethal infectious disease in the asia-pacific.patients with community-acquired acinetobacter baumannii (ab) pneumonia have been reported from subtropical countries. we investigated the epidemiology, clinical and microbiological characteristics of community-acquired pneumonia (cap) due to ab in singapore.200919909464
pathogens resistant to antibacterial agents.resistance to antimicrobial drugs is increasing at an alarming rate among both gram-positive and gram-negative bacteria. traditionally, bacteria resistant to multiple antimicrobial agents have been restricted to the nosocomial environment. a disturbing trend has been the recent emergence and spread of resistant pathogens in nursing homes, in the community, and in the hospital. this article reviews the epidemiology, molecular mechanisms of resistance, and treatment options for pathogens resistant ...200919909886
infections and treatment of wounds in survivors of the 2004 tsunami in thailand.on 26 december 2004, a tsunami devastated the west coast of thailand and caused 8457 injuries and 5395 deaths. data were collected from 26 december 2004 to 31 january 2005 at four public hospitals to describe the character and treatment of wounds of 523 persons who were injured during tsunami and sought medical treatment. wounds were contaminated with mud, sand, debris and sea water and had an infection rate of 66.5% (674/1013). most wounds (45%) had poly-microbial infection with gram-negative r ...200919912391
novel low-temperature-active phytase from erwinia carotovora var.carotovota accc 10276.a phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. therefore, this study used a degenerate pcr and tail pcr to clone a phytase gene from erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. the full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kda with a 50% amino acid identity to the klebsiella pneumoniae phytase ...200919884763
interspecies spread of klebsiella pneumoniae carbapenemase gene in a single patient.klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae, escherichia coli, and serratia marcescens were sequentially identified in a patient who underwent small bowel transplantation. molecular typing and plasmid analysis suggested that the kpc gene was acquired by e. coli, most likely from k. pneumoniae, and was subsequently transferred to s. marcescens.200919886795
the spread of klebsiella pneumoniae carbapenemases: a tale of strains, plasmids, and transposons. 200919886796
the in-vitro antimicrobial activity of some medicinal plants against beta-lactam-resistant bacteria.in effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria.200919858568
ventilator-associated nosocomial pneumonia in intensive care units in malaysia.the outcome indicator of nosocomial infection (ni) in the intensive care unit (icu) is used to benchmark the quality of patient care in malaysia. we conducted a three-year prospective study on the incidences of ventilator-associated pneumonia (vap), risk factors, and patterns of the microorganisms isolated in three icus.200919858572
why hypothetical protein kpn00728 of klebsiella pneumoniae should be classified as chain c of succinate dehydrogenase?twenty percent of genes that encode for hypothetical proteins from klebsiella pneumoniae mgh78578 were identified, leading to kpn00728 and kpn00729 after bioinformatics analysis. both open reading frames showed high sequence homology to succinate dehydrogenase chain c (sdhc) and d (sdhd) from escherichia coli. recently, kpn00729 was assigned as sdhd. kpn00728 thus remains of particular interest as no annotated genes from the complete genome sequence encode for sdhc. we discovered kpn00728 has a ...200919859792
[bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008].bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ...200919860320
risk factors and clinical impact of klebsiella pneumoniae carbapenemase-producing k. pneumoniae.klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae is an emerging pathogen with serious clinical and infection control implications. to our knowledge, no study has specifically examined risk factors for kpc-producing k. pneumoniae or its impact on mortality.200919860564
cross-reaction between proteins of larrea divaricata cav. (jarilla) and proteins of gram-negative bacteria.larrea divaricata is an abundant plant of northwest of argentina used to treat different pathologies. we aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (jpce) of jarilla. we evaluated the cross reaction between jpce and whole cell-bacterial proteins (w-cbp) of escherichia coli, pseudomonas aeruginosa, proteus vulgaris, and klebsiella pneumoniae using a mouse anti-jpce serum. protein profiles of jpce and w-cbp were analyzed. for jpce, 18 bands ...200919874237
chemical composition and antimicrobial and antioxidant activities of mentha (longifolia l. and viridis) essential oils.the study was aimed to investigate essential oil chemical composition (gas chromatography/flame ionization detection [gc-fid] and gas chromatography/mass spectrometry [gc-ms]) and antioxidant (1,1-diphenyl-2-picrylhydrazyl free radical (dpph) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate [abts] assays) and antimicrobial (gram-positive and gram-negative bacteria, fungi, and yeast) activities of essential oils extracted from leaves of mentha longifolia l. and mentha viridis. gc-ms analysis ...200919895481
[ctx-m-15-producing klebsiella pneumoniae: a change in the epidemiology of esbl.]klebsiella pneumoniae is frequently involved in nosocomial outbreaks worldwide. high level of resistance is common for these bacteria leading to reduce antibiotic treatments and prolonged hospital stay for patients. resistance determinants are often located on plasmids. during the 1980-1990s, esbl encoding genes belonged to the tem and shv type. from the early 2000s, a new trend was observed with esbl of the ctx-m type being increasingly described in k. pneumoniae, and more particularly ctx-m-15 ...200919896297
joint extraintestinal manifestations in ulcerative colitis.an extraintestinal manifestation (eim) very often occurs in ulcerative colitis (uc) patients. eim modifies the natural course of uc and decreases the quality of life in these patients. the aim of this study was to analyze clinical and laboratory findings in uc patients with joint eim. 319 uc patients were examined. among them were 131 (41.1%) patients with distal uc, 102 (32.0%) suffered from left-sided uc and 86 (26.9%) had pancolitis. 95 (29.8%) uc patients had joint eim. arthritis correlated ...200919897966
usefulness of pcr melting profile method for genotyping analysis of klebsiella oxytoca isolates from patients of a single hospital unit.the development of rapid and simple typing methods is required in order to identify possible sources of human exposure to opportunistic pathogens. klebsiella spp. belongs to a group of bacteria that are opportunistic pathogens responsible for an increasing number of multiresistant infections in hospitals. recently, we showed the high genetic diversity of k. oxytoca using a large collection of strains isolated from the patients of several hospitals in poland over a 50-year period. our results sho ...200919899618
comparison of 3 phenotypic-detection methods for identifying plasmid-mediated ampc beta-lactamase-producing escherichia coli, klebsiella pneumoniae, and proteus mirabilis strains.plasmid-mediated ampc beta-lactamases (pabls) have been detected in the strains of escherichia coli, klebsiella spp., proteus mirabilis, and salmonella spp. pabls may be difficult to detect and might interfere in the therapeutic and infection-control processes. although several pabl-detection methods based on phenotypes have been reported, the clinical and laboratory standards institute currently does not recommend a routine detection method for pabls. the aim of this study is to compare the per ...200919893354
[etiological factors of community acquired urinary tract infections in hospitalized patients].etiological factors of community-acquired urinary tract infections (uti) are specific for age, gender, season, complication of uti and type of uti. their prevalence and susceptibility to antimicrobial agents shows geographic and time variability.200920088156
[gemifloxacin for the treatment of uncomplicated urinary infections (acute cystitis)].uncomplicated urinary infections are a significant and growing cause of morbidity amongst young women. commonly these infections are caused by escherichia coil or staphylococcus saprophyticus. escherichia coil is resistant to several empirical antibiotics: amoxicilin, trimetoprima-sulfametozaxol and, more recently, to some more old flouroquinolons. gemifloxacin is a flouroquinolon with an excellent in vitro activity against many community acquired bacteria which cause respiratory or urinary infe ...200920077882
epidemiology and susceptibility to antimicrobials in community, hospital and long-term care facility bacteremia in northern israel: a 6 year surveillance.identification of pathogens and their susceptibility to antimicrobials is mandatory for successful empiric antibiotic treatment.200920077944
[investigation of ctx-m type beta-lactamases in klebsiella pneumoniae clinical isolates by phenotypic and molecular methods].rapid spread of ctx-m type extended-spectrum beta-lactamases (esbl) between the members of enterobacteriaceae receives attention increasingly all throughout the world. the aim of this study was to investigate the presence of ctx-m type esbl in klebsiella pneumoniae clinical strains by phenotypic and molecular methods. a total of 217 non-repetitive k. pneumoniae strains isolated from clinical specimens (152 urine, 20 sputum, 17 wound swabs, 13 blood, 9 tracheal aspirate, 3 csf and 3 conjunctival ...200920084908
[factors effecting the duration of hospitalization and mortality in patients with community-acquired pneumonia].community-acquired pneumonia (cap) is a common infectious disease with high morbidity and mortality. in this study, demographic features, underlying conditions, causative pathogens and factors affecting length of hospital stay and mortality were retrospectively investigated in patients who were diagnosed as cap and followed-up in our unit between january 2005-december 2007. among 97 patients 65 (67%) were male, 32 (33%) were female and the mean age was 62.7 (age range: 18-94) years. patients wer ...200920084912
[investigation of antibacterial activity of sertralin].sertralin is a psychotropic drug which acts by inhibiting the selective serotonin re-uptake in the synaptic area. previous studies have shown that some antidepressant agents have antibacterial activity. the aim of this study was to investigate the in vitro antibacterial activity of sertralin. a total of 224 bacterial strains isolated from clinical specimens together with standard control strains were included to the study. the antibacterial activity of sertralin was determined by microdilution m ...200920084919
profile of bacteria and fungi on money coins.to determine the quantity and quality of bacterial and fungi on money coins and to identify those that could pose a public health risk.200920084997
impact of limited cephalosporin use on prevalence of methicillin-resistant staphylococcus aureus in the intensive care unit.methicillin-resistant staphylococcus aureus (mrsa) has emerged as a leading pathogen causing nosocomial infections. many studies have shown that the restricted use of antibacterials is associated with a decline in resistance. to establish whether an intervention protocol designed to limit the use of cephalosporins can lower mrsa infection rates and impact on gram-negative bacteria susceptibility in an intensive care unit (icu), we conducted a prospective, non-randomized, before-after interventio ...200920071286
intravenous 2-chloroadenosine protects balb/c mice from klebsiella pneumoniae b5055-induced sepsis by modulating the pro-inflammatory immune response.sepsis is a severe inflammatory immune response of the host against an infectious agent or its product i.e. lipopolysaccharide (lps). therefore, targeting the immune system during sepsis may lower the morbidity and mortality associated with sepsis. the present study shows the protective immunomodulatory action of 2-chloroadenosine (2-cado) in k. pneumoniae b5055 induced sepsis in male balb/c mice. sepsis was induced by implanting the fibrin-thrombin clot containing known amount (10(2)cfu) of kle ...200920071287
pathogen incidence and antibiotic resistance patterns of catheter-associated urinary tract infection in children.the aim of this study was to characterize the pathogens and their antibiotic susceptibilities in children with catheter-associated urinary tract infection (cauti) in order to optimize empirical antibiotic therapy and prophylaxis. from 2001 to 2006, 895 children with an indwelling catheter from 3 hospitals in china were included in this study, of whom 335 (37.4%) had cauti. antimicrobial susceptibility testing of 450 bacterial isolates was performed using the clsi broth and kirby-bauer agar dilut ...200920071290
infection of brindley sacral anterior root stimulator by pseudomonas aeruginosa requiring removal of the implant: long-term deleterious effects on bowel and urinary bladder function in a spinal cord injury patient with tetraplegia: a case report.we report infection of brindley sacral anterior root stimulator in a spinal cord injury patient, who ultimately required removal of the implant. the consequences of failed implantation were severe constipation, and loss of reflex penile erection and bladder emptying.200920062610
plasmid-mediated quinolone resistance determinants in urinary isolates of escherichia coli and klebsiella pneumoniae in a large singapore hospital.at the time of the study, 3 plasmid-borne qnr determinants (qnra, qnrb and qnrs) and 1 plasmid-borne aminoglycoside-modifying enzyme determinant that confers quinolone resistance (aac(6')-ib-cr) had been described in the literature.200920052442
[antimicrobial treatment of nosocomial intra-abdominal infections--new treatment options with tygecycline].nosocomial, intra-abdominal infections are extremely serious conditions, considering possibilities for their early diagnosis, as well as for their effective therapy. multiresistant bacteria (enterobacteriacae producing extended-spectrum beta-lactamases - esbl escherichia coli, klebsiella species, vancomycin-resistant enterococci [vre], and methicillin-resistant staphylococcus aureus [mrsa]) are frequently isolated as pathogens of these infections. tygecycline is among the novel wide- spectrum an ...200920052927
prevalence of extended- spectrum beta-lactamase producing escherichia coli and klebsiella spp in a neonatal intensive care unit.this study reports the prevalence of extended-spectrum b-lactamase producing escherichia coli and klebsiella spp among septicemic neonates. over a five year period, there were 94 isolates of klebsiella spp and e .coli. of these, 68 (72.3%) were esbl producers. forty (80%) of the klebsiella spp isolates produced esbl as compared to 28 (63.6%) of e.coli.200920061592
trends in antimicrobial susceptibility of gram-negative bacteria isolated from blood in jakarta from 2002 to 2008.this study examined the susceptibility of gram-negative bacteria in the bloodstream to antimicrobials with the aim of providing information relevant to the guidance of therapy.200920061679
antimicrobial susceptibility of pathogens isolated from more than 10,000 patients with infectious respiratory diseases: a 25-year longitudinal study.the study group on antimicrobial susceptibility of pathogens isolated from respiratory infections was established in 1981 in japan to elucidate trends in such susceptibilities in patients with infectious respiratory diseases; the group has conducted nationwide research in collaboration with 21 medical institutions. examination of more than 10,000 patients by 2005 allowed a summary of study findings. streptococcus pneumoniae started to become resistant to penicillin g in the 1990s, and the isolat ...200920012724
antimicrobial efficacy assessment of multi-use solution to disinfect hydrophilic contact lens, in vitro.to evaluate the efficacy of disinfecting solutions in hydrophilic contact lenses (cl).200920027398
meckel's diverticulum manifested by a subcutaneous abscess.this case report describes an extremely rare complication of a meckel's diverticulum: enterocutaneous fistula of the diverticulum. the presence of meckel's diverticulum is a well known entity, but subcutaneous perforation of the diverticulum is very rare. here we report the case of a patient with the complaint of a right lower quadrant abscess, preoperatively diagnosed as enterocutaneous fistula, which was determined intraoperatively to be a fistula resulting from meckel's diverticulum.200920027688
investigation of a community outbreak of typhoid fever associated with drinking water.this report is about the investigation of an outbreak of typhoid fever claimed three human lives and left more than 300 people suffered within one week. the aim of this report is to draw the attention of global health community towards the areas that are still far from basic human essentialities.200920021691
nosocomial infections prevalence study in a serbian university hospital.nosocomial infections (ni) are a serious health problem resulting in an enromous burden of excess morbidity and mortaliti rates, and health care costs. the aim of this study was to assess the prevalence of ni and to identify groups of patients at special risk for ni in the university clinical center, kragujevac, serbia.200920017416
[vicissitude of antibiotic sensitivity of gram negative bacilli in patients with infection in emergency departments in beijing during 2005 to 2007].to investigate changes in antibiotic sensitivity of gram negative bacilli infections among emergency patients in large hospitals in beijing during 2005 to 2007.200920042141
bacteriological evaluation of pre-cut fruits sold in kano metropolis, kano state, nigeria.one hundred and fifty (150) pre-cut fruit samples comprising of pineapples (50), paw-paw (50) and watermelon (50) at the point of stand retail outlets were tested by standard bacteriological methods to determine bacterial contamination of the fruits. out of these 150 examined 136 (90.67%) were contaminated with bacteria. the bacterial distribution were; escherichia coli 69 (46.00%), staphylococcus aureus 29 (19.33%), salmonella species 13 (8.67%), proteus species 18 (12.00%), enterobacter aeroge ...200920000065
streptococcus pneumoniae coinfection is correlated with the severity of h1n1 pandemic influenza.initial reports in may 2009 of the novel influenza strain h1n1pdm estimated a case fatality rate (cfr) of 0.6%, similar to that of seasonal influenza. in july 2009, however, argentina reported 3056 cases with 137 deaths, representing a cfr of 4.5%. potential explanations for increased cfr included virus reassortment or genetic drift, or infection of a more vulnerable population. virus genomic sequencing of 26 argentinian samples representing both severe and mild disease indicated no evidence of ...200920046873
characteristics of aac(6')-ib-cr gene in extended-spectrum beta-lactamase-producing escherichia coli and klebsiella pneumoniae isolated from chungnam area.concomitant quinolone resistance in extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae is a crucial problem in the clinical management of infections. in foreign countries, the fluoroquinolone acetylating aminoglycoside-(6)-n-acetyltransferase (aac[6']-ib-cr) gene, a novel plasmid-mediated quinolone resistance determinant has been reported to occur in conjunction with qnr. we aim to investigate the prevalence and characteristics of concomitant aac(6')-ib-cr and qnr expression in ...200920046086
infant botulism mimicking an acute abdomen.botulism is the acute, flaccid paralysis caused by a neurotoxin produced by clostridium botulinum. in the infant, clinical symptoms are usually unspecific such as poor feeding, weak suck, feeble cry, drooling, followed by a symmetric, descending, flaccid paralysis beginning with the cranial nerve musculature. the initial symptoms of the disease are often similar to several diseases and therefore differential diagnosis is very difficult and rarely suspected by the physician. since 2004 only 22 ca ...200920046108
epidemiology of invasive klebsiella pneumoniae with hypermucoviscosity phenotype in a research colony of nonhuman primates.invasive klebsiella pneumoniae with hypermucoviscosity phenotype (hmv k. pneumoniae) is an emerging human pathogen that, over the past 20 y, has resulted in a distinct clinical syndrome characterized by pyogenic liver abscesses sometimes complicated by bacteremia, meningitis, and endophthalmitis. infections occur predominantly in taiwan and other asian countries, but hmv k. pneumoniae is considered an emerging infectious disease in the united states and other western countries. in 2005, fatal mu ...200920034435
antibiotic sensitivity pattern of gram negative bacilli isolated from the lower respiratory tract of ventilated patients in the intensive care unit.lower respiratory tract infections (lrtis) are the most frequent infections among patients in intensive care units (icus).200920040812
ventricular assist device infection necessitating device exchange following extensive myocardial resection.a 14-year-old boy undergoing long-term mechanical circulatory support with a left ventricular assist device (lvad) developed myocardial abscess resulting from cannula exit-site infection. this critically ill patient was successfully treated with lvad replacement and omentopexy following extensive myocardial debridement. although the use of artificial materials in the face of active infection is not ideal, other options including lvad explantation or urgent heart transplantation are not always fe ...200920035401
the influence of omentectomy on bacterial clearance: an experimental study.the influence of an omentectomy on peritoneal defense mechanisms and its clinical consequences have not been fully elucidated. in the present study, we aimed to investigate the influence of omentectomy on bacterial growth in blood and tissue specimen cultures in rats with experimental peritonitis.200920037870
[study of nosocomial bacillary meningitis admitted in the clinic of infectious diseases iaşi on a 20 years period].to analyze the clinical features, etiology and evolution of nosocomial bacillary meningitis admitted to the clinic of infectious diseases iaşi between 1988 and 2007.200920191822
clinical and bacteriological characteristics of pyogenic liver abscess in non-diabetic patients.diabetes mellitus is an important risk factor for klebsiella pneumoniae liver abscess, but many patients with pyogenic liver abscess (pla) do not have diabetes. this study was conducted to compare the clinical characteristics and prognostic factors of k. pneumoniae pla with that caused by other organisms in non-diabetic patients.200920182667
high prevalence rate of multidrug resistance among nosocomial pathogens in the respiratory care center of a tertiary hospital.there is an increasing need for respiratory care centers (rccs) for patients who require prolonged mechanical ventilation after intensive care unit (icu) stay. nosocomial infections occur at a high rate in icus, but there have been few studies of nosocomial infections in rccs in taiwan.200920182669
microbiology and epidemiology of brain abscess and subdural empyema in a medical center: a 10-year experience.brain abscess and subdural empyema are the 2 most common forms of intracranial pyogenic infections. predisposing factors and etiological agents may change with time. this study examined the epidemiological features of these conditions.200920182670
activity of ertapenem, ciprofloxacin, ceftriaxone, piperacillin-tazobactam, and ampicillin-sulbactam against 12 common clinical isolates of community-acquired bacteremia.to compare the antimicrobial activities of ertapenem, ciprofloxacin, ceftriaxone, piperacillin-tazobactam, and ampicillin-sulbactam against 12 common organisms that cause community-acquired bacteremia and to identify the most active agents for the treatment of extended-spectrum beta-lactamase (esbl)-producing escherichia coli and klebsiella pneumoniae.200920182674
rapidly fatal bacteremic pneumonia caused by klebsiella pneumoniae with k1 hypermucoviscosity phenotype in a previously healthy young man receiving levofloxacin treatment.fatal bacteremic klebsiella pneumoniae pneumonia is commonly encountered in alcoholic and diabetic patients. this report describes a previously healthy young man with rapidly fatal bacteremic pneumonia caused by k. pneumoniae serotype k1, complicated by septic shock and multiple organ dysfunction.200920182675
[endemic intravenous fluid contamination in pediatric wards].to determine the rate of contamination of intravenous solutions and injection ports in pediatric patients.200920184097
synthesis and antibacterial assessment of n-[4-(4-substituted phenyl)-1,3-thiazol-2-yl]-1,3,5-triazin-2-amine.in a wide search program towards new and efficient antibacterial agents, we assessed the extent to which physicochemical properties can be exploited to promote antibacterial activity associated with a series of substituted s-triazine. the synthesized compounds (1a-12b) were subsequently screened for their in vitro antibacterial activity against three gram positive (bacillus subtilis, bacillus cereus, staphylococcus aureus) and three gram-negative microorganism (salmonella typhi, escherichia coli ...200920177467
antibacterial and antioxidant activity of methanol extract of evolvulus nummularius.to evaluate the antibacterial and antioxidant activity of methanol extract of evolvulus nummularius (l) l.200920177496
notes on the microbiology of cholesteatoma: clinical findings and treatment.anomalous proliferation of the cholesteatoma epithelium is caused by extrinsic factors such as toxins or bacterial antigens combined with lytic enzymes, lymphokines and cytokines released from the inflammatory infiltrate. this could explain the close relationship between the aggressiveness of cholesteatoma and repeated bacterial superinfection, therefore it is very important to know the bacteria involved in order to control the regrowth of skin following surgery, reduce the aggressive potential ...200920161877
[genotypic relatedness of klebsiella pneumoniae strains isolated from clinical specimens].fourty six isolates of k. pneumoniae were studied. the strains were cultured from different clinical specimens received from children hospitalized at university hospital in bydgoszcz. one strain was cultured from hospital environment. production of esbl was assessed using double disk synergy test and confirmatory test. the genomic dna was extracted from the strains separated by pfge after digesting with xbai endonuclease. production of esbl was detected in 73.9% of k. pneumoniae isolates. molecu ...200920120923
molecular and biochemical characterization of three bacterial symbionts of fruit fly, bactrocera tau (tephritidae: diptera).characterization of three bacterial symbionts (bc1, bc2 and bc3) of fruit fly bactrocera tau including morphological, biochemical and 16s rdna (rrs gene) analysis was done to determine their taxonomic position. morphological and biochemical characterization placed two bacteria (bc1, klebsiella oxytoca and bc2, pantoea agglomerans) into family enterobacteriaceae and the third one (bc3, staphylococcus sp.) into family staphylococcaceae. 16s rdna gene sequence comparison with the available ncbi dat ...200920118612
resistances of antibiotics and heavy metals in enterobacteriaceae spp. isolated from gills and intestines of achanthobrama marmid (heckel,1843) from sir dam lake turkey.a total of 94 bacteria, associated with wild achanthobrama marmid (heckel, 1843) in sir dam lake of turkey identified. subsequently selected isolates were characterized and identified to the genus level the 94 members of enterobacteriaceae were isolated in the gills and intestines, and among the isolates, e. coli were represented at a rate of 55%, shigella spp. at a rate of 21%, salmonella spp. at a rate of 9%, citrobacter spp. at a rate of 9%, klebsiella spp. at a rate of 3% and proteus spp., a ...200920112859
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