Publications

inherited thyroxine excess: a serum abnormality due to an increased affinity for modified albumin.	further analysis of sera from euthyroid subjects with dominantly-inherited, elevated serum total thyroxine (t4) and free t4 index but with normal free t4 levels was performed as an extension of a previous study (hennemann et al., 1979a). scatchard analysis and isoelectric focusing of whole sera and purified serum fractions suggest that this t4 excess is due to increased t4 binding by modified serum albumin. recognition of this syndrome and appreciation that the free t4 index does not reflect the ...	1981	6797763
t3 kinetics in euthyroid graves' disease patients who exhibit hyper-responsiveness to trh after radioiodine treatment.	a major reduction in t3 turnover has been demonstrated previously in clinically hypothyroid patients. we have used non-compartmental (nc) and monocompartmental (mc) analysis to study ten patients with graves' disease who, following treatment with radioactive iodine (rai), are now clinically euthyroid but who showed hyper-responsiveness to trh although serum t3 and t4 concentrations are within the normal range. t3 production rate (pr), metabolic clearance rate (mrc) and fractional-turnover (k) we ...	1981	6797765
the effect of clomiphene citrate on sex hormone binding globulin in normospermic and oligozoospermic men.	the effect of clomiphene citrate (cg) on sex hormone binding globulin (shbg) was studied in 10 oligozoospermic patients with varicocele and 6 normospermic men. plasma shbg, testosterone (t), oestradiol (e2), fsh, lh, prolactin (prl), thyroxine (t4) and 17-oh-progesterone (17-oh-p) were determined before and during medication. shbg concentration rose from 38.1 +/- 18.3 to 54.3 +/- 16.0 nmol/l (p less than 0.01), while t and e2 showed significant increases from 31.2 +/- 10.8 nmol/l and 24.6 +/- 5. ...	1981	6797955
sizes of bacteriophage t4 early mrna's separated by preparative polyacrylamide gel electrophoresis and identified by in vitro translation and by hybridization to recombinant t4 plasmids.	we determined the sized of specific t4 prereplicative nrna's by preparative polyacrylamide gel electrophoresis, and we used the following two techniques to identify specific gene transcripts; cell-free protein synthesis accompanied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to distinguish t4 polypeptides and hybridization to recombinant plasmids containing t4 dna of known genetic composition. in our first analysis, the use of nonsense and in-phase deletion mutants allowed unamb ...	1981	6459465
studies on escherichia coli mutants which block bacteriophage morphogenesis.	we have previously reported the isolation of e. coli groe mutants, which block lambda head morphogenesis. further analysis of these mutants showed that many are temperature-sensitive for bacterial growth or block the growth of the unrelated phages t4 and t5. we have established the existence of a second groe gene, which we call groes, that is different from the previously described groe gene encoding a 65,000-mr polypeptide (georgopoulos and hohn, 1978; hendrix and tsui, 1978) and which is renam ...	1981	6460256
organization of the bacteriophage t4 tail fiber gene cluster 34-38.	a correlation of the genetic, functional, and structural maps of the t4 tail fiber gene cluster has been achieved by analysis of lambda derivatives carrying genes 34-38. 31 recombinants carrying different parts of the t4 tail fiber gene cluster were identified by a marker rescue screen of 300 lambda t4 recombinant clones, generated by restriction of partial cytosine-containing t4 dna with e cori or with hindiii and ligation into appropriately cleaved lambda replacement vectors. extensive genetic ...	1981	6460257
responder cells in the human autologous mixed lymphocyte reaction.	isolated human t4+ cells proliferate in the autologous mixed lymphocyte reaction (amlr), whereas isolated t8+ cells do not. however, in the presence of interleukin 2 or t4+ cells, the t8+ cells demonstrated substantial proliferation. these studies suggest that t8+ cells recognize signals from autologous non-t cells, but require an additional factor for the subsequent proliferative response. since this stimulus can be provided by t4+ cells, the amlr appears to constitute an inducer circuit. diffe ...	1981	6459343
distribution of t cell subsets in human lymph nodes.	a series of t cell-specific monoclonal antibodies was used to determine the location of t lymphocyte subpopulations in frozen sections of human lymph nodes by means of an immunoperoxidase technique. the majority of cells in the paracortical regions were reactive with anti-t1 and anti-t3 antibodies, which define all mature peripheral t cells. in contrast, the majority of cells within primary follicles were unreactive with anti-t1 and anti-t3 antibodies, but were reactive with anti-ia and anti-igm ...	1981	6450262
interactions between phage lambda replication proteins, lambda dna and minicell membrane.	gentle methods for minicell lysis and lysate fractionation have been elaborated: lysis by t4 lysozyme without detergents, and fractionation by equilibrium sedimentation in a metrizamide density gradient, both at low ionic strength. in the lysates of phage-lambda-infected minicells the lambda dna, trapped at a prereplicative step [witkiewicz, h. and taylor, k. (1979) biochim. biophys. acta 564, 31-36], appeared in two peaks of different buoyant densities: as a membrane-bound and a free lambda dna ...	1981	6451425
autoantibody to an immunoregulatory inducer population in patients with juvenile rheumatoid arthritis.	the human inducer (t4(+)) and reciprocal cytotoxic/suppressor (t5(+)/t8(+)) subsets have been defined by monoclonal antibodies. in the present study, we examined the relationship of naturally occurring anti-t cell autoantibodies found in patients with active juvenile rheumatoid arthritis (jra) to these subsets. in one approach, normal t cells were treated with anti-t4 or anti-t8 to eliminate the corresponding subset of cells and then analyzed for reactivity with jra sera. it was found that jra s ...	1981	6451634
immunodeficiency associated with loss of t4+ inducer t-cell function.	we investigated the immune function of a patient with anergy and acquired hypogammaglobulinemia. despite normal numbers of b cells and t4+ inducer and t5+ suppressor t cells, this patient's lymphocytes did not produce immunoglobulin, proliferate in response to soluble antigens, or generate helper factors in vitro. in addition, her t4+ t cells did not express la molecules after stimulation by soluble antigen. in mixing experiments, her t cells did not induce immunoglobulin secretion by b cells fr ...	1981	6451805
[indications for oral trh in a study of 998 patients (author's transl)].	the authors determined values for t3, t4, t7, t3ria and basal tsh, as well as tsh stimulation after intravenous administration of trh in 998 patients. the test was negative in 259 patients (26%); in 51 of these patients (5.1%) no clinical or laboratory reasons could be found (low- or non-responders). in this group of patients the trh test was repeated using a hundred times greater dose orally; in 27 cases (54%) of the low- or non-responders the oral test proved positive. in total, the trh test w ...	1981	6452363
regulation of in vitro primary anti-dnp antibody production by functional subsets of t lymphocytes in man.	a primary in vitro antibody response to dnp-klh by peripheral blood lymphocytes in culture was developed. optimal anti-dnp antibody production, as measured by solid phase radioimmunoassay occurred at a cell density of 1 x 10(6), and an antigen concentration of 5 to 10 micrograms per culture when pbl was incubated with the antigen for 5 days and then cultured in the absence of dnp-klh for 4 additional days. the antibody produced was shown to be primarily of the igm isotype and was specific for dn ...	1981	6453899
[effect of low temperatures of the survival and intracellular multiplication of escherichia coli bacteriophages].	the purpose of this work was to investigate the survival and intracellular growth of escherichia coli bacteriophages phi x174, t3 and t4 subjected to freezing down to -196 degrees c. the survival was 98.4% for phage t3, 80.55% for phage phi x174, and 65.13% for phage t4. single growth cycles, dna and protein biosynthesis did not change in phages phi x174 and t3 after freezing. if bacteria were infected with phage t4 subjected to freezing, the rates of phage dna and protein synthesis decreased, t ...	1981	6454057
surface phenotype of japanese adult t-cell leukemia cells characterized by monoclonal antibodies.	we studies the surface phenotype and the functional activities of leukemic cells from three patients with japanese adult t-cell leukemic (atl) using the panel of ok and anti-tac monoclonal antibodies, which react with differentiation antigens and define functionally distinct t-cell subsets or activated and terminally differentiated t cells. the phenotype of atl cells were determined to be okt1+t3+t4+t10+t5-t8-okla1-, although cells from two patients suppressed pokeweed mitogen (pwm) induced norm ...	1981	6455129
interactions of bacteriophage t4-coded gene 32 protein with nucleic acids. iii. binding properties of two specific proteolytic digestion products of the protein (g32pi and g32piii).		1981	6455528
lymphoid subpopulations of peripheral blood and spleen in untreated hodgkin's disease.	a panel of monoclonal antibodies with well-defined specificities were used as probes to investigate the phenotypes and lineage of circulating lymphoid cells and splenocytes in untreated patients with hodgkin's disease. a significant relative and absolute reduction in t cells (anti-t3+) was found only in patients with b-symptoms. there was no alteration in the fraction of helper (anti-t4+) or cytotoxic/suppressor (anti-t5+) t cells circulating in peripheral blood when compared to normals, nor was ...	1981	6456062
deficiency of suppressor t cells in the hyperimmunoglobulin e syndrome.	the status of suppressor t cells (ts) was assessed in seven children with the hyper ige syndrome (recurrent staphylococcal infections, eczematous skin rash, and elevated serum ige) to determine whether a deficiency in ts is associated with increased ige synthesis. when circulating t cells and their subsets were enumerated with the aid of monoclonal antibodies that identify t cells (t3), helper/inducer t cells (t4), and suppressor/cytotoxic t cells (t8), there was a selective deficiency of t3+ ce ...	1981	6456275
genetic complementation by cloned bacteriophage t4 late genes.	bacteriophage t4 containing nonsense mutations in late genes was found to be genetically complemented by four conjugate t4 genes (7, 11, 23, or 24) located on plasmid or phage vectors. complementation was at a very low level unless the infecting phage carried a denb mutation (which abolishes t4 dna endonuclease iv activity). in most experiments, the infecting phage also had a dena mutation, which abolishes t4 dna endonuclease ii activity. mutations in the alc/unf gene (which allow dcmp-containin ...	1981	6456362
postnatal development of masticatory organs in rats ii. effects of hormones on the postnatal development of the m. masseter superficialis.	the effects of l-thyroxine (t4; 0.2 microgram/g body weight) an hydrocortisone (hc 5 microgram/g body weight) on the m. masseter superficialis in developing rats were investigated. administration of t4 and/or hc to rats for 5 consecutive days from day 5 after birth induced precocious increase in diameter of the muscle fibers, increase of sdh activity and differences of the activity in various fibers and precocious disappearance of fibers with edta-insensitive atpase activity. the effects of thes ...	1981	6456679
molecular mechanisms of substitution mutagenesis. an experimental test of the watson-crick and topal-fresco models of base mispairings.	the proteins coded by bacteriophate t4 replication genes 32, 41, 43, 44, 45, 61, and 62 together can replicate phi x174 dna templates very efficiently. the fidelity of this in vitro replication reaction has been measured using an infectivity assay. the product molecules have the same specific infectivity as the template dna. when an amber mutant dna template is used, no increase in the frequency of revertants is seen even after more than 60 duplications in vitro. by using imbalances in the conce ...	1981	6457040
[effect of change of habitat (sea and fresh water) on in vivo thyroglobulin synthesis in atlantic glassed eels (anguilla anguilla l.)].	thyroid biosynthesis in glassed eels (anguilla anguilla l.) was studied to establish whether salinity changes could affect it, when they live in sea water or in fresh water containing 125i. aqueous extrait of homogenized cephalic heads of glassed eels contains an iodinated protein 17-19 s having thyroglobulin-like properties and including iodotyrosins (mit and dit) and thyroid hormones (3 and t4). biosynthesis of this proteins is roughly twice more important in fresh water than in sea water at 1 ...	1981	6457669
[nycthemeral variations of thyroxine levels in the quail: effect of ambient temperature].	plasma t4 levels were determined at 2 hr intervals in male quail reared under 18 l6-24 : 6d and 26 +/- 1 degrees c. low thyroxinemia (4.7 -5.7 mg/ml) was observed during the light phase of the photoperiod. t4 levels were sharply increasing during the first part of the dark period, up to a peak (9.2 ng/ml) at 3 a.m., and decreased again by the last part of the night. exposure to 4 degrees c for 30 min resulted in different effects on thyroxinemia according to the time of the day. during the light ...	1981	6457671
enumeration of t lymphocyte subsets by monoclonal antibodies in young and aged humans.	the percentage and absolute number of peripheral blood mononuclear cells reactive with monoclonal antibodies identifying mature thymocytes and t cells (t3+), helper/inducer cells (t4+), and suppressor/cytotoxic cells (t8+) was determined in 19 young (mean age 35 yr) and 31 elderly (mean age 72 yr) individuals. the percent representation but not the absolute number of t cells (t3+) declined significantly (p less than 0.001) in the elderly, and the decline was attributable to both an absolute and ...	1981	6457861
molecular cloning of the t4 genome: organization and expression of the tail fiber gene cluster 34--38.	lambda t4 derivatives that carry t4 tail fiber genes 34-38 have been isolated and characterized by genetic, structural and functional analysis. 32 lambda t4 recombinants were identified by a marker rescue screen of 310 lambda t4 clones generated by restriction of partial cytosine-containing t4 dna with either hindiii or ecori and ligation into appropriately cleaved lambda vectors. these tests defined 15 recombinant classes with respect to the contiguous stretches of genome recovered. restriction ...	1981	6457960
comparative expression of t9, t10, and ia antigens on activated human t cell subsets.	in the present study, the expression of three surface molecules t9, t10, and ia, which are found on activated t lymphocytes, was examined utilizing monoclonal antibodies and indirect immunofluorescence. these antigens were shown to appear on t lymphocytes in a defined temporal sequence which was dependent on the specific triggering stimulus. moreover, when t cells were fractionated into individual subsets of t4+ inducer and t8+ cytotoxic/suppressor lymphocytes, the expression of all three molecu ...	1981	6458585
deoxynucleoside [1-thio]triphosphates prevent proofreading during in vitro dna synthesis.	the contribution of proofreading to the fidelity of catalysis by dna polymerases has been determined with deoxyribonucleoside [1-thio]triphosphate substrates. these analogues, which contain a sulfur in place of an oxygen on the alpha phosphorus, are incorporated into dna by dna polymerases at rates similar to those of the corresponding unmodified deoxynucleoside triphosphates. the fidelity of dna synthesis was measured with phi x174 am3 dna; reversion to wild type occurs most frequently by a sin ...	1981	6458818
effects of thyroid hormones and thyrotropin-releasing hormone on thyrotropin biosynthesis by mouse pituitary tumor cells in vitro.	mouse thyrotropic tumor cells grown in primary culture were shown to synthesize tsh and proteins, as determined by the incorporation of radioactive proline into immunoprecipitable tsh and trichloroacetic acid-precipitable proteins. the net tsh content of the cells and medium determined by ria is also increased during 24 h of incubation, and newly formed hormone is detected in the medium within 1 h after the addition of proline tracer. to study the effect of t4 and t3 on tsh synthesis, cultures w ...	1981	6780319
sulfate transfer to thyroid hormones and their analogs by hepatic aryl sulfotransferases.	the transfer of sulfate to thyroid hormones and their analogs by a monkey hepatocarcinoma cell extract was compared to this reaction as catalyzed by two homogeneous aryl sulfotransferases from rat liver. the thyroid hormones 3,5,3'-triiodo-l-thyronine and l-t4 as well as analogs of these hormones, were used as substrates. while these compounds vary widely in their capacity as sulfate acceptors, the catalytic abilities of the three enzyme preparations generally reflect a broad pattern of overlapp ...	1981	6778682
the sex-related difference in serum thyrotropin concentration is androgen mediated.	studies were carried out on various aspects of hypothalamic-pituitary-thyroid function in normal and gonadectomized adult male and female rats. consistent increases in the serum tsh concentration and the serum tsh response to trh were observed in the male rat compared to values in the female. orchiectomy induced a decrease in the serum tsh concentration and the serum tsh response to trh, and these functions were equal in gonadectomized male and female rats. oophorectomy did not affect basal and ...	1981	6778683
metoclopramide increases prolactin release and milk secretion in puerperium without stimulating the secretion of thyrotropin and thyroid hormones.	to explore the effect of metoclopramide (mc) on the secretion of prl, tsh, and thyroid hormones (t3 and t4) and on defective lactation, 17 mothers with poor lactation were treated with oral mc (10 mg. three times daily) for 3 weeks starting 18-141 days post partum. after a pause of 1 week, the medication was given for a further 2 weeks. the breast milk yield was monitored objectively before and during the trial. furthermore, iv stimulation tests with mc (10 mg) and trh (200 microgram) were done ...	1981	6780593
thyroid gland function and pituitary tsh reserve in patients with cystic fibrosis.	in view of the reported enhanced sensitivity to iodide-induced hypothyroidism in patients with cystic fibrosis, studies were carried out to determine the possible mechanism of this abnormality. the intrathyroid organification of iodide, as assessed by the iodide-perchlorate discharge test, was normal in patients with cystic fibrosis, strongly suggesting that an organification defect was not present. the serum tsh response to trh was not significantly different from the response in normal childre ...	1981	6780673
tsh response to trh in euthyroid, hypercholesterolemic patients treated with graded doses of dextrothyroxine.	in an attempt to determine the minimum dose of d-thyroxine (d-t4) which will suppress pituitary tsh response to trh, we have treated 6 euthyroid, hypercholesterolemic patients with graded doses of d-t4. tsh response was suppressed in 3 patients with 3 mg and in the remaining 3 patients with 4 mg d-t4 administered once daily. the mean tsh suppressive dose of 3.5 mg, as determined in this study, is considerably less than the 6 mg daily dose given to patients treated with d-t4 in the coronary drug ...	1981	6780753
[standardisation of trh test for the exact determination of thyroxine treatment (author's transl)].	a trh test was performed on 50 patients treated on a daily thyroxine dose of 50-200 microgram. results were compared with a test performed at the earliest three weeks on the same thyroxine dosage, but the latter having been interrupted for 24 hours. in the first case t4 was 8.96 +/- 2.9 microgram/dl, in the second 7.08 +/- 2.0 microgram/dl; t3 in the first case 1.28 +/- 0.3 ng/ml, in the second 1.03 +/- 0.25 ng/ml. this indicates that with both thyroid hormones the levels were higher on the day ...	1981	6781851
thyroxine-binding globulin: investigation of microheterogeneity.	preparations of t4-binding globulin (tbg) from human serum was performed using only two affinity chromatography steps. purity of the protein was demonstrated by a single band in overloaded disc and sodium dodecyl sulfate electrophoresis, equimolar binding to t4, and linearity in sedimentation velocity run. the molecular weight was calculated to be 60,000 +/- 3,000 daltons (n = 3), the sedimentation coefficient was 3.95s, and the stokes' radius was 37 a. the amino acid composition was found to be ...	1981	6782114
simultaneous determination of free thyroxine and capacity of thyroxine-binding globulin.	a simple method is described for the simultaneous determination of capacity thyroxine-binding of globulin (tbg) and free thyroxine concentration (ft4). the ratio of bound to free t4 (b/f) is first determined for two total-t4 concentrations using a sephadex g-25 competitive-binding technique. tbg capacity and ft4 can both the calculated assuming a known value of affinity constant of tbg. the method is linear over a twenty-fold serum dilution. ft4 calculated is identical to that calculated using t ...	1981	6782200
altered serum levels of thyroxine, triiodothyronines and diiodothyronines in endogenous depression.	serum levels of thyroxine (t4), 3,3',5-triiodothyronine (t3), 3,3',5-triiodothyronine (rt3), 3,5-diiodothyronine (3,5-t2), 3,3'-diiodothyronine (3,3'-t2) and 3',5'-diiodothyronine (3',5'-t2) were studied in 80 patients with endogenous depression before and after electroconvulsive treatment (ect). compared to the values found after recovery, the patients when depressed had significant increased serum levels of t4, rt3, 3,3'-t2 and 3',5'-t2. serum concentrations of t3 and 3,5-t2 were not significa ...	1981	6781193
the effect of growth hormone on the plasma levels of t4, free-t4, t3, reverse t3 an tbg in hypopituitary patients.	the plasma concentrations of thyroxine (t4), free thyroxine (free-t4), triiodothyronine (t3), reverse triiodothyronine (rt3), tsh and thyroxine-binding globulin (tbg) were measured in 19 children suffering from idiopathic growth hormone deficiency. blood was taken before and one month after growth hormone treatment. ten patients were hypothyroid (group 1) and 9 were euthyroid (group 2). the basal t3 and rt3 levels correlated well with the t4 concentrations. free-t4 levels were very low in all th ...	1981	6782790
influence of carbamazepine on serum thyroxine and triiodothyronine in patients with epilepsy.	hypothyroidism induced by anti-epileptic drug treatment gave rise to thyroid function test studies in patients treated with carbamazepine (cbz) only. in 42 patients on long-term cbz treatment thyroxine (t4), free t4-index (ft4i), and triiodothyronine (t3) concentrations in serum were significantly lower than in controls, while triiodothyronine uptake (t3u) and thyrotropin (tsh) concentrations did not differ between patients and controls. in 12 patients starting on cbz, means t4, calculated ft4 a ...	1981	6782820
binding of ribosomes to linear and circular forms of the 5'-terminal leader fragment of tobacco-mosaic-virus rna.	the sequence of the 5'-terminal leader fragment preceding the aug codon in the rna of tobacco mosaic virus (tmv), tomato strain, sps isolate, has been determined. this rna, similarly to the rnas of the u1 and dahlemense strains of tmv [kukla et al. (1979) eur. j. biochem. 98, 61--66] has the 7-methylguanosine(5')triphospho(5')guanosine cap separated from the initiation codon by a long stretch of nucleotides devoid of guanosine residues. the rnase-t1-resistant 73-nucleotide-long leader fragment o ...	1981	6783406
excision of pyrimidine dimers from nuclear deoxyribonucleic acid in ultraviolet-irradiated dictyostelium discoideum.	a sensitive endonuclease assay was used to study the fate of pyrimidine dimers introduced by ultraviolet irradiation into the nuclear deoxyribonucleic acid of the cellular slime mold dictyostelium discoideum. analysis of the frequency of t4 endonuclease v-induced single-strand breaks by alkaline sucrose gradient sedimentation showed that strain nc4 (rad+) removed greater than 98% of the dimers induced by irradiation at 40 j/m2 (254 nm) within 215 min after irradiation. hps104 (radc44), a mutant ...	1981	6965095
studies of thyroid hormone and methimazole levels in patients with graves' disease on a standardized anti-thyroid drug regimen.	the outcome of a standardized carbimazole (cmi) regimen was evaluated in fifty-four patients with graves' disease. triiodothyronine (t3) and thyroxine (t4) serum values were determined before and throughout the first 6-8 months of therapy. before therapy, decreasing t4/t3 ratios were found with increasing t3 levels, reflecting a relative increase in t3 production compared with t4 in thyrotoxicosis. high pretreatment t3 values were comparatively common among patients in whom a fixed cmi dosage ha ...	1981	6895351
long-acting thyroid stimulator (lats) and long acting thyroid stimulator protector (lats-p) in untreated thyrotoxicosis.	the prevalence of serum lats and lats-p in untreated graves' disease has been studied in eighty-three patients with thyroid overactivity; twelve (14%) were found to have significant levels of lats and lats-p whilst fifty-nine (72%) had significant levels of lats-p alone. twelve (14%) had neither activity. there was a very wide range in both activities. lats was invariably found in association with a significantly greater concentration of lats-p. there was no correlation between lats-p and thyroi ...	1981	6895353
lymphocytic thyroiditis and diabetes in the bb/w rat. a new model of autoimmune endocrinopathy.	the bio breeding/worcester (bb/w) rat develops spontaneous insulin-dependent diabetes mellitus secondary to lymphocytic infiltration and destruction of the pancreatic beta-cells. this destructive process in the pancreas has been postulated to be based on a thymus-dependent cell-mediated autoimmune process. in view of the well recognized association in man of diabetes mellitus and another autoimmune endocrinopathy, chronic thyroiditis (hashimoto's thyroiditis), the present studies were carried ou ...	1981	6895508
the effects of 1,25-dihydroxycholecalciferol, parathyroid hormone, and thyroxine on trabecular bone remodeling in adult dogs. a histomorphometric study.	the effects of 1,25-dihydroxycholecalciferol (1,25-(oh)2d3), parathyroid hormone (pth), and l-thyroxine (t4) on trabecular bone remodeling were evaluated by histomorphometric methods in adult female beagle dogs. intravenous 1,25-(oh)2d3 (1.25 micrograms/day in equally divided doses) was administered intermittently for 6 days and withdrawn 14 days for three complete cycles. pth was administered intravenously (2.5 u/kg/day) in divided doses 6 hours apart for 60 days. thyroxine was given orally (1. ...	1981	6895577
excision repair and patch size in uv-irradiated bacteriophage t4.	we determined the average size of excision repair patches in repair of uv lesions in bacteriophage t4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. the average patch was small, approximately four nucleotides long. in control experiments with the denv1 excision-deficient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted dna and prevented nicking by the uv-specific endonucleases of micrococcus luteus and bacteriophage t4.	1981	6895652
["silent" marker effect in t4-phage recombination].	effects of the silent sites of heterozygosity on genetic recombination have been studied in crosses between the markers localized in the proximal segment of riib gene of t4 phage. it was found that silent markers could inhibit as well as stimulate genetic recombination. for the expression of these marker effects, the distance between the silent marker and those for which recombination was being monitored, appeared to be critical. there were no marker interference in the crosses in which an influ ...	1981	6941911
[contribution of correction to genetic recombination in t4-phage measured in 3-factor crosses over indicator distances].	marker-dependence of recombination in t4 phage was studied in crosses over indicator distance between the markers localized in the short proximal segment of riib gene. in three-factor crosses a-b+c- x a+b-c+, the frequencies of recombinants arising as a result of correction of the central marker b to the wild type allele (chi b leads to +) have been determined. the measured values chi b leads to + were found to be in good agreement with the analogous values determined in two-factor crosses. this ...	1981	6941913
on the molecular basis of transition mutations: frequencies of forming 2-aminopurine.cytosine and adenine.cytosine base mispairs in vitro.	we address the question of whether substituting 2-aminopurine (apur) in place of adenine (ade) in dna can increase the frequency of base mispairing with cytosine. using dna polymerase alpha to measure the rates of inserting deoxycytidine and thymidine nucleotides in direct competition with each other for apur or ade sites on synthetic copolymer dna templates, we observe that the ratio of dcmp to dtmp insertion is increased by a factor of at least 230 when apur replaces ade on a poly(da) template ...	1981	6942407
tricyclic antidepressants do not alter thyroid hormone levels in patients suffering from a major affective disorder.	abnormalities of thyroid function have been associated with affective disorders, and treatment with thyroid hormones or drugs that alter thyroid hormone levels can change the course of an affective disorder. therefore, we investigated the possibility that mood-altering effects of tricyclic antidepressants would be mediated by alterations in thyroid hormones (t3 and t4) levels. in a group of 11 patients with affective disorders, tricyclic antidepressants did not alter serum t3 and t4 levels. we c ...	1981	6943598
epidermoid carcinoma compared with adenocarcinoma of the anal canal.	sixty-five cases of anal carcinoma have been treated in the past 20 years - 44 adenocarcinomas, 15 epidermoid carcinomas and 6 others. the sex ratio of male to female was 2:13 among those with epidermoid carcinoma and 27:17 in the adenocarcinoma group. there was no significant difference between the five-year survival associated with both kinds of carcinoma, though adenocarcinomas invaded more deeply and were larger. lymph node metastases were found in 60% of cases in each group, but inguinal me ...	1981	6947779
two types of replication proteins increase the rate at which t4 dna polymerase traverses the helical regions in a single-stranded dna template.	we have recently developed an in vitro dna synthesis system in which a synthetic heptaribonucleotide pairs with a unique site on a single-stranded fd dna molecule and thereby primes the growth of new dna strands from this single point (huang, c.-c., and hearst, j. e. (1980) anal. biochem. 103, 127-139). in this report, we use this system to investigate the mechanism by which various bacteriophage t4 dna replication proteins stimulate the t4 dna polymerase. we find that with the "polymerase acces ...	1981	6971292
effect of thyroxine, testosterone, and corticosterone on nerve growth factor (ngf) and epidermal growth factor (egf) concentrations in adult female mouse submaxillary gland: dissociation of ngf and egf responses.	testosterone propionate (tp) and corticosterone acetate (ca) were administered alone and in combination with t4 to assess the effect on submaxillary gland (smg) nerve growth factor (ngf) and epidermal growth factor (egf) concentrations in adult female mice. mice were treated for 5 or 10 days. smg ngf, and egf concentrations were measured by specific ria techniques. mean smg ngf (0.68 +/- 0.08 microgram/mg protein) and egf (0.58 +/- 0.05 microgram/mg protein) concentrations were similar in contro ...	1981	6972865
interaction of lactoferrin, monocytes, and t lymphocyte subsets in the regulation of steady-state granulopoiesis in vitro.	colony-stimulating activities (csa) are potent granulopoietic stimulators in vitro. using clonogenic assay techniques, we analyzed the degree to which mononuclear phagocytes and t lymphocytes cooperate in the positive (production/release of csa) and feedback (inhibition of csa production/release) regulation of granulopoiesis. we measured the effect of lactoferrin (a putative feedback regulator of csa production) on csa provision in three separate assay systems wherein granulocyte colony growth o ...	1981	6972953
changes of iodothyronine levels in plasma after acute and long term hypokinesia (unforced restriction) in rats.	groups of 8 rats each were subjected to hypokinesia (unforced restriction) in plexiglass cages for 1, 5 and 9 days in first experiment and groups of 10 rats each were subjected to 1, 5, 14 and 60 days of hypokinesia in a second experiment. in addition, in a second experiment two groups of 10 animals were subjected first to hypokinesia for 60 days and then kept in regular group cages for 7 and 21 days, respectively. in each experiment a control group was used which was not subjected to restrictio ...	1981	6973461
chloramphenicol inhibits hormone-dependent induction of cytoplasmic mrna coding for the mitochondrial enzyme, carbamyl phosphate synthetase, in rana catesbeiana tadpoles.	in tadpoles injected with thyroxine (t4), synthesis of carbamyl phosphate synthetase 1 is induced so that within 7-8 days this single polypeptide represents one of the most abundant proteins in the liver. translational assays in vitro showed that liver rna from control animals had very low levels of translatable mrna coding for the enzyme whereas carbamyl phosphate synthetase mrna activity was prominent in liver from tadpoles which had been treated with t4 for just 2 days. when the primary trans ...	1981	6974007
abnormalities of t cell maturation and regulation in human beings with immunodeficiency disorders.	a series of monoclonal antibodies to t cell surface antigens were used to characterize peripheral lymphoid populations from patients with a variety of immunodeficiency diseases. several disorders of t cell differentiation were observed to occur in severe combined immunodeficiency. one subtype of severe combined immunodeficiency was associated with failure to develop lymphocytes that express any thymus specific antigens, another with failure to differentiate beyond the early prothymocyte-thymocyt ...	1981	6974177
metabolism of thyroxine in rana catesbeiana tadpoles during metamorphic climax.	previous studies have indicated that premetamorphic tadpoles do not convert t4 to t3 to a measurable extent (1). the present study was performed to determine whether a t4 5'-monodeiodinating system is acquired at later stages of development. [125i]t4 metabolism in vivo was determined in tadpoles at most stages of prometamorphosis and metamorphic climax and, for comparison, in premetamorphic tadpoles. the conversion of [125i]t4 to [125i]t3, as indicated by the presence of an 125i-labeled product ...	1981	6974641
derivation of human t-cell leukemias.	cell surface antigenic changes occur during the stages of t-cell ontogeny in several animal model systems. in the present study, multiple monoclonal antibodies were developed to human thymocyte and t-cell surface differentiation antigens as a means of probing t-cell ontogeny in humans. it was shown that three distinct states of human intrathymic differentiation exist and that the mature inducer (t4+) and suppressor (t5+/th2+)t-cell subsets arise at specific points during these maturation events. ...	1981	6975159
addition, deletion, and substitution of long nonhomologous deoxyribonucleic acid segments by genetic transformation of haemophilus influenzae.	a complete ecori digest of haemophilus influenzae phage hp1 deoxyribonucleic acid (dna) was mixed with incomplete digests of various h. influenzae r plasmids, sealed with t4 ligase, and transformed into an hp1 lysogen. most of the chloramphenicol- and tetracycline-resistant transformants did not produce phage although they possessed all the phage genes examined. they also did not transfer antibiotic resistance by conjugation. dna lysates from them transformed other lysogens to resistance and to ...	1981	6975273
t cell growth factor-enhanced pha response of human thymus cells: requirement for t3+ cells.	human thymocytes normally proliferate poorly in pha-stimulated cultures, as measured by thymidine uptake. their response increases when supernatant factors from mitogen-stimulated human lymphocyte cultures (sup) are added. the cells that proliferate in pha + sup-stimulated cultures continue to divide if they are resuspended with fresh sup every 3 to 4 days. pha-stimulated thymocytes are deficient in the production of the t cell growth factor interleukin 2 (il 2). the phenotype of thymocytes prio ...	1981	6975307
possible mechanism of action of cholesterol enriched diet on the thyroid.	groups of male sprague dawley rats aged 60 days were fed either a control diet (cd) or the same diet enriched with 1% cholesterol (chd) for 30 days. in chd fed animals an increase of 131i uptake by thyroid, pb131i and tsh level in plasma was found, while the level of thyroxine (t4) was unchanged and that of triiodothyronine (t3) was decreased. in the animals treated with t3 (20 micrograms i.p. daily) a decrease of 131i uptake by thyroid and of t4 and tsh level in plasma was found irrespectively ...	1981	6976263
the use of salmonella schottmulleri for mapping and separation of human lymphocyte subpopulations.	human lymphocyte subpopulations (b cells, b1, b2, t1, t2, t3, and t4 cells; our denomination) have been previously identified and isolated by bacterial adherence and functional differences between them have been demonstrated. here we examined the binding properties of salmonella schottmulleri to human lymphocytes in peripheral blood smears and found that it binds to more lymphocyte subpopulations, namely b, t1, t2 and t3 cells, than any bacteria previously tested. thus, using only four bacteria: ...	1981	6976306
placental transfer of maternal anti-rabbit igg causing falsely elevated tsh levels in neonates.	two infants were found to have markedly increased tsh levels, 104 and 154 mu/l of plasma, respectively, in a routine screening programme for congenital hypothyroidism. the recall limit used was 50 mu/l of plasma. on follow-up, both infants were clinically euthyroid and had normal serum t4 and t3. the elevated tsh levels were confirmed only with some commercial radioimmunoassay kits--but not with others. similar results were obtained in tsh assays of samples from their mothers, who had no other b ...	1981	6976675
surgery and adjuvant chemotherapy with high-dose methotrexate and folinic acid rescue for infiltrating tumors of the bladder.	thirty-three patients with category t3 or t4 bladder cancer were treated by partial or total cystectomy followed by 6 to 12 courses of high-dose methotrexate (hdmtx) with folinic acid rescue. survival at 2 years was 90%. two patients were treated palliatively with hdmtx alone, one of whom is alive and disease free after 35 months.	1981	6976831
enhancement of dmba induced mammary tumours by intermittent whole body hyperthermia (42 degrees c).	wistar female rats bearing dmba induced mammary tumours were subjected to whole body hyperthermia 42 degrees c dry heat exposure for 15 minutes daily for 6 weeks. the control group was maintained at a room temperature of 25 degrees c. hyperthermia induced significant growth stimulation of breast tumour compared to the controls. plasma estradiol was slightly decreased while total t4 and tsh values remained unchanged in heat stressed rats. plasma prolactin was significantly increased together with ...	1981	6807669
elevated levels of circulating carcinoembryonic antigen in hypothyroidism.	the level of circulating carcinoembryonic antigen (cea) was measured in 148 patients with various thyroid diseases. a significantly high frequency of positive cea was observed in hypothyroid patients with hashimoto's disease, but the serum cea levels were not correlated with the serum calcitonin concentrations in hashimoto's disease. the cea levels were inversely correlated with the serum t4 concentrations (p less than 0.001) and were positively correlated with the serum tsh concentrations (p le ...	1981	6893991
acquired refractoriness to thyroid hormone action in treated toxic nodular goiter.	a case of apparent target organ refractoriness to thyroid hormones has been observed during the recovery phase of toxic nodular goiter after 131i therapy. the serum pbi was normal before the onset, but elevated serum pbi and t4 concentrations were observed during the toxic phase, and after recovery, with elevated t3 as well. the case differed in important respects from the congenital, hereditary disorder known as the refetoff syndrome.	1981	6894170
triiodothyronine generation from thyroxine in human thyroid: enhanced conversion in graves' thyroid tissue.	conversion of t4 to t3 was studied in normal, graves', and neoplastic thyroid tissues obtained at surgery. homogenates of human thyroid follicular adenoma and carcinomas were incubated with [125i]t4 and dithiothreitol (dtt) at 37 c for 60 min under n2 gas. t3 formation was assessed by measuring [125i]t3 formed, using paper chromatography. in the second series of experiments, the suspensions of the 100,000 x g pellet of normal thyroid tissues adjacent to the tumor and graves' thyroid tissues were ...	1981	6894450
treatment of hyperthyroidism with sodium ipodate (oragrafin) in addition to propylthiouracil and propranolol.	ten patients with hyperthyroidism due to graves' disease were treated with sodium ipodate (1 g daily) in addition to propranolol (p) plus propylthiouracil (ptu; 100 mg every 8 h) and were compared with a control group of 8 patients treated with p and ptu. patients on p and ptu had a mean (+/- sem) basal free t3 index of 387 +/- 59 (normal, 70--160) compared with that of 409 +/- 47 (p greater than 0.05) in the sodium ipodate group. the respective basal free t4 index values (normal, 4.5-10.9) were ...	1981	6894929
[clinical results of the treatment of ovarian carcinoma following 16 years of experience with the chemotherapy resistance test (author's transl)].	a prospective study on the results of chemotherapy selected by the treatment chemotherapy resistance test of limburg an krahe (oncobiogram) of 191 cancers of the ovary from the same department from the years 1962-1978 is presented. in 152 cases (79.6%) surgical or radio-therapy was impossible or insufficient because of the advanced spread of the tumour (t4 of figo 3 and 4). nevertheless in the hopeless state t4 nx m1b (n = 89) a two years survival rate of 58% was obtained following the selective ...	1981	6908863
[a report about 311 ovarian carcinomas, treated at the "universitäts-frauenklinik des klinikums mannheim der universität heidelberg" from 1960--1978 (author's transl)].	in the medical histories no hints could be found, suggesting a definite risk-group for ovarian carcinoma. symptoms and complaints are often uncharacteristic and no methods for early recognition are known. the therapy of the ovarian cancer is an operation, combined with chemotherapy, or radiation considering to the stage of the tumour. most important for the prognosis is the degree of expansion, besides age of the patients and the histological pattern of the tumour. the three-years-survival rate ...	1981	6908876
radical irradiation for carcinoma of the pyriform sinus.	radiation therapy is sometimes prescribed as an alternative to partial or total laryngopharyngectomy in the initial management of carcinoma of the pyriform sinus. forty-two patients were irradiated for cure and have a follow-up period of 2-14 years. local control by irradiation alone was 11 of 14 for t1, 6 of 10 for t2, 3 of 6 for t3, and 1 of 7 t4. three t2 failures were salvaged by laryngopharyngectomy for an ultimate control of 9 of 10 for t2. management of the neck is critical since 79% of t ...	1981	7464405
thyroid growth-blocking antibodies in primary myxoedema.	adult primary myxoedema is usually due to an autoimmune thryoiditis characterized by progressive shrinking of the thyroid gland, loss of epithelium, dense infiltration by sensitized lymphocytes and plasma cells with final replacement of the gland by a fibrous scar. antibodies directed against thyroglobulin (tgha) and microsomal antibodies (mcha), detectable years before the onset of hormonal failure, and cell-mediated immune mechanisms contribute to the pathogenesis. when 90% of the gland is des ...	1981	7464924
[effectiveness of various hormonal preparations in adults with hypothyroidism (author's transl)].	among more than 7000 adult patients with suspected dysthyroidism in who radioimmune assays were performed, the authors have selected 21 patients with high tsh hypothyroidism who had assays before and after replacement therapy with various preparations prescribed by their g.p. the results in this group of 61 treatments of at least 3 weeks duration were as follows: (1) under thyroid extract or thyroglobulin (21 treatments), the condition persisted in the majority of patients; (2) under thyroxine ( ...	1981	7465359
carcinoma of the oropharynx.	179 consecutive cases of carcinoma of the oropharynx (excluding the tonsillar region) were treated by irradiation from 1960 through 1976. all patients with stage i tumor survived five years, compared with 56% for stage ii, 62% for stage iii, and 37% for stage iv. 58% of the primary lesions were controlled by irradiation; the rate ranged from 93.8% for t1 to 37.1% for t4, with a similar decrease by t category regardless of the site of origin. surgery contributed to salvage of recurrences, but the ...	1981	7465846
tamoxifen therapy in postmenopausal advanced breast cancer: efficacy at the primary tumor site in 46 evaluable patients.	forty-six evaluable postmenopausal patients with locally advanced, inoperable t3-t4 breast carcinoma were treated with tamoxifen 10-20 mg twice daily for a period at least 6 weeks. eight patients (17%) had an objective response at the primary tumor site after 6 weeks of treatment. improvement of response with a further single tamoxifen therapy was observed in 7 patients, resulting in an overall objective response in 14 of 46 (30%). median duration of response was 8 months (range 2-24). no respon ...	1981	7281242
a possible role of arginase in the regulation of polyamine biosynthesis in the rat thyroid.	effect of chronic methylthiouracil (mtu) treatment on the thyroid arginase activity and thyroidal concentration of arginine, ornithine and other amino acids was studied in the rat. the activity of thyroid arginase increased significantly at 15 days of mtu treatment and the elevated enzyme activity was reduced to normal by l-thyroxine (t4) injection. the thyroidal concentration of polyamines was increased by mtu and decreased by t4 with the exception of spermine. the thyroidal concentration of ar ...	1981	7282268
squamous cell carcinoma of the maxillary sinus.	a retrospective analysis of 59 cases of squamous cell carcinoma of the maxillary sinus, which were treated either with radiation alone or combined surgery and radiation, was performed. fifty-six cases (95%) had t3 and t4 lesions. our results show that six (66.6%) of nine of t3 and t4 lesions were controlled by combined therapy, while radiation alone controlled 15 (34%) of 44 of t3 and t4 lesions. five-year actuarial survival of t3 and t4 patients treated with combined therapy was 75% and 33.3%, ...	1981	7469880
failure of thyroid hormones to maintain the normal lipoprotein pattern in rats after removal of the pituitary gland.	hypophysectomy was found to increase low density lipoprotein (ldl) cholesterol in rats from 0.18 to 1.18 mmol/l in 1 week, while very low density lipoprotein (vldl) and high density lipoprotein (hdl) cholesterol decreased simultaneously from 0.08 to 0.03 mmol/l and from 2.12 to 1.01 mmol/l, respectively. serum total cholesterol levels remained unchanged. thyroid supplementation (t3 or t4) with doses causing a euthyroid state did not fully correct the lipoprotein pattern. the increase of ldl caus ...	1981	7470194
arginine vasopressin release following surgical operations.	a study of plasma arginine vasopressin (avp) concentrations in 16 patients undergoing uncomplicated cholecystectomy was carried out to determine the relative importance of the various stimuli to avp secretion during and after an operation. eight of the 16 patients were given a high epidural blockade from t4 and t12 and blood samples were taken before, at frequent intervals during and for two days after operation for determination of plasma avp and osmolarity; systolic blood pressure was measured ...	1981	7470828
thyroid hormone pattern and aggressiveness. (isolated mice compared with dominant and submissive).	the aggressiveness induced by the forced isolation of animals may be mediated by endocrine changes. the present study focuses on the possible mediation of the pituitary-thyroid axis in the mouse (mus musculus). the blood levels of the hormone fractions examined (t4, t3 and rt3) in isolated animals showed a significant increase in comparison with the individuals which were classified from an ethological point of view as submissive, whereas in comparison with the individuals which were classified ...	1981	7268272
neonatal screening for hypothyroidism in scotland: results of a pilot study.	we have assessed the feasibility of screening for neonatal hypothyroidism based on thyroxine (t4) or thyrotrophin (tsh) assays on dried blood spots collected for the phenylketonuria (pku) screening programme in scotland. the tsh assay was both more sensitive and specific than t4 in detecting primary hypothyroidism and was sufficiently rugged and rapid for routine application. using a labelled antibody assay for tsh, 99.7 per cent of the first 30,000 babies tested had values less than 25 mu/l, an ...	1981	7268398
[prognosis and therapy in case of hypopharynx carcinoma (author's transl)].	a report is given on 84 patients with hypopharynx carcinomas who were treated between 1960 and 1979 at the radiotherapy department of würzburg. there is a high rate of recurrences after all therapy methods. 65 patients were only irradiated, ten patients were irradiated after and nine before surgical procedure. the five-year survival time of the patients irradiated only is 43% for the stages t1 and t2 and 4% for the stages t3 and t4, the statistical difference being significant. the problem of tu ...	1981	7268815
in vitro packaging of bacteriophage t4 dna.		1981	7269246
effect of cycloheximide on iodothyronine formation in vitro.	the unique inhibitory effect of cycloheximide (ch) on the coupling of iodotyrosines was examined in vitro. rat thyroid lobes were incubated for 8 h under our improved condition. in the presence of 10(-4) - 10(-3) m ch, the per cent uptake of 131i decreased, proportionate synthesis of [131i]mit increased slightly, and that of [131i]t4 or [131i]t3 decreased markedly. the incorporation of medium 127i into t4 or t3 during the 8 h incubation period decreased markedly, but was fairly constant into mit ...	1981	7270006
[serum levels od d-thyroxine in euthyroidism and hypothyroidism (author's transl)].	serum levels of d-thyroxine (d-t4) and l-thyroxine (l-t4) were determined by use of a newly developed method for the stereospecific determination of these isomers in 8 patients with diffuse non-toxic goiter and 2 hypothyroid patients following administration of increasing doses of d-thyroxine. during the 3-day observation period a significant decrease of serum-tsh takes place. d-t4 levels are highest 4 hours after administration of the tablets and approach basal values within 24 hours in euthyro ...	1981	7270017
serum thyroid hormones in two yugoslav districts with different iodine intake.	serum thyroid hormones were measured in two areas of yugoslavia, one with relatively low iodine intake, zagreb (urinary iodide 111 +/- 36 microgram/g creatinine), and the island of brac with higher iodine intake (247 +/- 76 microgram/g creatinine). the serum concentration of t4 and t3 in two groups were not different. these data confirm previous findings that iodine intake within accepted normal range, is not a factor in determining serum thyroid hormone levels.	1981	7274165
partition of thyroid hormone among erythrocyte cytosol, erythrocyte membrane and human plasma binding sites.	the partition of thyroid hormone among binding sites on plasma proteins, erythrocyte (rbc) membranes and cytoplasmic proteins has been examined in the context of recent direct estimates of intracellular rbc thyroid hormone content. isotopic t3 and t4 uptakes by washed rbcs are identical (greater than 80% of tracer at 4 hr), but rbc binding when rbcs are suspended in plasma favors t3 (10% uptake vs. 2.5% for t4). the rbc cytosol: plasma t3 ratio is 1 : 21.	1981	7274993
relationship between the purity and molecular weight of calf thymus dna.	the molecular weight and purity of calf thymus dna, prepared by different procedures of isolation, were determined. the highest molecular weight was obtained by the method of blin and stafford (12 x 10(7)). this dna contained 17% protein. the further purification of this dna led both to increase of purity and to reduction of the molecular weight. the relationship between molecular weight and achieved purity was found to be a monotonic sigmoid-shape function ranging between the molecular weights ...	1981	7275016
specific anti-thyroxine antisera induced by thyroxine sensitized liposomes.	specific anti-thyroxine rabbit antisera were generated from complete freund's adjuvant with liposomes consisting of sphingomyelin, cholesterol, dicetylphosphate and 5-n-thyroxine-2,4-dinitrophenyl-phosphatidylethanolamine (t4-dnp-pe). spin membrane immunoassay technique was used to measure the sensitivity and specificity of these antisera. addition of 10 to 70 ng of l-thyroxine produced significant inhibition of immune lysis. addition of l-3,3',5-triiodothyronine (t3) up to 800 ng showed no cros ...	1981	7275165
thyroid hormone changes in obese subjects during fasting and a very-low-calorie diet.	total fasting was compared with vlcd (1.28 mj, 300 kcal/day; 56 g protein, 12 g cho) in 14 euthyroid obese patients, selected as matched pairs, over a period of 28 days. the weight loss was significantly greater during fasting than during the vlcd (16.5 kg vs. 12.7 kg). the basal metabolic rate of (bmr) showed a significant decrease (25 per cent) during total fasting, but was unchanged with the vlcd. with both diets there was a transient increase in t4, ft4 and ft4i, and a tendency for tsh to de ...	1981	7275468
thyroid function in patients with acute renal failure.	thyroid function was evaluated in a group of 36 patients with acute renal failure (arf) during the oliguric/anuric, polyuric and postpolyuric phase. serum thyroxine (t4) and triiodothyronine (t3) concentrations were significantly decreased in the oliguric/anuric phase, as compared with the mean values obtained in the post-polyuric phase and with controls. in contrast to t3 and t4, the concentration of serum reverse triiodothyronine (rt3) was elevated in the oliguric/anuric phase and normal in th ...	1981	7275524
partial exclusion of bacteriophage t2 by bacteriophage t4: an exclusion-resistant mutation in gene 56 of t2.	the early genes of bacteriophage t2 are partially excluded from the progeny of crosses between the related bacteriophages t2 and t4. this is due to complete exclusion from the progeny of six exclusion-sensitive sites in t2. a mutation [exr(56)1] in the sensitive site near t2 gene 56 renders the site partially resistant against exclusion. this paper describes the mapping of the exr(56)1 mutation. the mutation was mapped between two clusters of amber 56 mutations in t2, but mapping was not complet ...	1981	7276910
problems in screening for congenital hypothyroidism using thyroxine assays from small dried blood discs.	the feasibility of screening for neonatal hypothyroidism by initial thyroxine (t4) measurements from small (3.2 mm diam.) discs of dried heel or cord blood was assessed using double-antibody radioimmunoassay and solid-phase radioassay kits. adult heparinized venous blood or finger-prick capillary blood served as references. reproducibility of results was satisfactory in adult but not infant samples, probably because of problems in collecting and handling the latter. in comparative studies, most ...	1981	7277026
re-innervation of ganglia transplanted to the neck from different levels of the guinea-pig sympathetic chain.	thoracic and lumbar sympathetic ganglia from donor guinea-pigs were transplanted to the bed of an excised superior cervical ganglion in host animals. homotopic transplants of superior cervical ganglia served as controls. in this way the same set of preganglionic axons (the cervical sympathetic trunk) was confronted with ganglia from different levels of the sympathetic chain. re-innervation of the transplants was studied after 3-5 months. 1. neurones in ganglia transplanted from different levels ...	1981	7277232
crystallographic determination of the mode of binding of oligosaccharides to t4 bacteriophage lysozyme: implications for the mechanism of catalysis.		1981	7277499
relation between hen egg white lysozyme and bacteriophage t4 lysozyme: evolutionary implications.		1981	7277500
t3 and t4 carcinoma of the larynx--a retrospective study.	t3 and t4 lesions of the larynx were reviewed and the five year survival figures compared for radiotherapy, surgery, and combined therapy. glottic t3n0m0 lesions gave an acceptable five year survival figure of 66 per cent. radiotherapy is advised as the modality of treatment for t3n0m0 lesions. larger glottic and supraglottic lesions responded poorly to all modalities of treatment and a more aggressive treatment policy may be advisable.	1981	7277559
[studies on the measurement of serum thyroxine with "amerlex t4 ria" kit (author's transl)].		1981	7277879