Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| temperature dependence of the joining by t4 dna ligase of termini produced by type ii restriction endonucleases. | the temperature dependence of the t4 dna ligase-catalyzed joining of plasmid dna linearized by the action of haeiii, ecori and psti restriction endonucleases has been investigated by electron microscopy analysis. the extent of joining is maximal at 4 degrees and decreases with increasing temperatures following sigmoid-like curves. the temperature at which 50% of the maximal reaction is still observable increases going from dna termini without single-stranded overlaps (produced by haeiii) to term ... | 1981 | 6259621 |
| alteration of escherichia coli rnase d by infection with bacteriophage t4. | 1981 | 6260104 | |
| duplex dna binding activity of the avian myeloblastosis virus dna polymerase. | purified avian myeloblastosis virus dna polymerase has a strong binding affinity for closed circular double-stranded dna with no 3'-hydroxy termini. because of this affinity the dna polymerase can retain labeled native cole1 dna on nitrocellulose filters. when the reaction contains four enzyme molecules per cole1 molecule about 50% of the dna is retained. higher enzyme: dna ratios cause retention of nearly 100% of the dna. the binding activity comigrates with dna synthetic activity through ion-e ... | 1981 | 6260187 |
| joining of yeast alanine transfer ribonucleic acid half molecules to form a whole molecule by t4 rna ligase. | covalent joining of the two half molecules of trnaala by t4 rna ligase to form a reconstituted whole molecule was investigated. the two half molecules consisting, respectively, of residues 1-35 and 36-75 were prepared by partial degradation of trnaala with rnaase t1. the 5'-half molecule was treated with alkaline phosphatase to remove the 3'-terminal phosphate group, and the 5'-oh group of the 3'-half molecule was phosphorylated with [gamma-32p]atp by polynucleotide kinase. the two terminal nucl ... | 1981 | 6260188 |
| a new procedure for the simultaneous large-scale purification of bacteriophage-t4-induced polynucleotide kinase, dna ligase, rna ligase and dna polymerase. | a procedure for simultaneous large-scale purification of the bacteriophage-t4-induced polynucleotide kinase, dna ligase, rna ligase and dna polymerase has been developed. the method involves bacterial cell disruption by sonication, fractionation of cell extract with polymin p, salt elution from the polymin pellets, ammonium sulfate precipitation, and subsequent column chromatography purification of the enzymes. to enrich the enzyme content highly in the initial source non-permissive escherichia ... | 1981 | 6260493 |
| nonfunctional thyroglobulin messenger rna in goats with hereditary congenital goiter. | a goat strain with congenital goiter was studied as a model for human thyroid disoders. these goats were deficient in thyroglobulin (tg), the precursor protein of the thyroid hormones t3 and t4. rna coding for tg (tg-rna) was detected in reduced amounts in the goiters and was almost absent from the membranes, where tg is normally synthesized. this paper describes the preparation and characterization of a goat tg cdna plasmid and its use in the study of thyroglobulin gene expression in the goiter ... | 1981 | 6125553 |
| effect of prolonged administration of ranitidine on pituitary and thyroid hormones, and their response to specific hypothalamic-releasing factors. | we have studied, in a double blind controlled trial, 30 male patients with duodenal ulcer to evaluate the effect of prolonged oral administration of ranitidine (150 mg bd for 4 weeks), a new h2-receptor antagonist, on basal prl, lh, fsh and tsh concentrations, on their response to specific releasing hormones, and on basal and trh-stimulated levels of thyroid hormones. neither the basal levels of prl, fsh, lh and tsh, nor their response to stimulation with appropriate releasing hormone were affec ... | 1981 | 6120055 |
| characterization of the dna-dependent gtpase activity of t4 gene 41 protein, an essential component of the t4 bacteriophage dna replication apparatus. | the product specified by t4 bacteriophage gene 41 is known from genetic analyses to be essential for phage dna replication in vivo. correspondingly, the purified gene 41 protein is an essential component of an efficient in vitro dna replication system reconstructed from seven purified t4 replication proteins; it is required both for the synthesis of short rna primers (in conjunction with the t4 gene 61 protein) and for the rapid unwinding of the double-helical dna template at a replication fork. ... | 1981 | 6110662 |
| characterization of rna primer synthesis in the t4 bacteriophage in vitro dna replication system. | in the presence of any natural single-stranded dna, the bacteriophage t4 gene 41 and gene 61 proteins catalyze the synthesis of a group of pentaribonucleotides. these rna molecules are homogeneous in chain length, but heterogeneous in sequence. in the presence of the other five t4 replication proteins, these pentaribonucleotides are utilized with high efficiency as primers for de novo dna chain initiations on the lagging strand in the t4 dna replication process. by using a two-dimensional fracti ... | 1981 | 6110663 |
| neurotransmitter control of hypothalamic-pituitary-thyroid function in rats. | the possible roles of monoamine neurotransmitters in the regulation of the hypothalamic-pituitary-thyroid axis were examined in the rat. rats were treated acutely and repeatedly with drugs which are presumed to alter neurotransmitter functional activity. these drugs include neurotransmitter precursors (tryptophan and l-dopa), synthesis inhibitors (p-chlorophenylalanine and alpha-methyltyrosine), uptake inhibitors (desipramine and zimelidine) and lithium carbonate. the hormone levels measured wer ... | 1981 | 6112152 |
| postinfection control in t4 bacteriophage infection: inhibition of the rep function. | we suggest that the general mechanism by which t4 phage turns off host macromolecular synthesis involves specific phage proteins which react with key components in the synthetic pathway. support for this mechanism exists for the inhibition of host rna synthesis. here we note that the host rep function was inhibited after t4 phage infection. since rep functions are known to be involved in host dna replication, inhibition of rep might alter the course of host dna replication. | 1981 | 6112279 |
| [subclinical hypothyroidism in infancy (author's transl)]. | clinical symptoms of hypothyroidism (constipation, macroglossia, umbilical hernia, typical face hyperbilirubinaemia) were found in a 4 months old infant. body height and activity were normal and the skin did not show the typical dryness. total-thyroxin (t4) level was normal as well as basal thyrotropin (tsh), only after stimulation with thyrotropin releasing hormone (trh) an exaggerated response was observed. these hormonal findings are typical for subclinical hypothyroidism, a well known distur ... | 1981 | 6114419 |
| serum thyroglobulin in thyroid cancer. | serum thyroglobulin (tg) was measured in 274 patients with differentiated thyroid cancer; 266 had previous thyroidectomy, which had been followed by ablative iodine-131 in 183 cases. neither the presence nor the titre of anti-tg antibodies appeared to affect tg assays. serum tg reflected the presence or absence of cancer in 83% of 164 patients not receiving thyroxine (t4). this concordance improved to 97.5% in 158 patients tested while receiving t4. 34 patients in remission were tested both on a ... | 1981 | 6115202 |
| rheumatoid arthritis: a disease of t-lymphocyte/macrophage immunoregulation. | in rheumatoid arthritis the synovial membrane has many of the characteristics of a hyperactive, immunologically-stimulated lymphoid organ. the basis of this hyperactivity is poorly understood. highly specific antisera to human ia-like (hla-dr) antigens and monoclonal antibodies (okt series) to various t-lymphocyte subsets were used to analyse both the normal and the rheumatoid synovium and to compare it with normal lymph nodes. in rheumatoid arthritis the synovium acquires an infiltrate with mic ... | 1981 | 6116956 |
| [metabolic disorders in severe head injuries]. | we have studied some central and peripheral hormones in patients suffering from severe head injury. there exists a significant increase in plasma noradrenaline at the beginning of the apallic syndrome and the values remained raised until the reintegration of the neurological symptomatic set in. besides there exists a decreased stimulation of growth hormone (hgh) excretion after arginine infusion at different stages of the treatment. testosterone, triiodothyronine (t3) and thyroxine (t4) show low ... | 1981 | 6117511 |
| effect of iodination of a village water-supply on goitre size and thyroid function. | an iodinator was fitted to the existing gravity-fed water-supply of a remote village in sarawak, malaysia, where goitre was endemic. within nine months, the prevalence of goitre had been reduced from 61% to 30%, with 79% of goitres showing visible reduction in size. all subjects were clinically euthyroid before and nine months after the start of iodination, although pre-treatment serum thyroid-stimulating hormone (tsh) concentrations varied from normal up to 24 mu/l. before treatment basal serum ... | 1981 | 6118679 |
| endocrinologic and psychologic aspects of galactorrhea associated with normal menstrual cycles. | twelve cases of galactorrhea in women with normal menstrual cycles who were radiologically free of any pituitary adenomas were investigated. determinations were made for serum thyroid-stimulating hormone (tsh), t3 resin uptake (t3ru), total thyroxine by radioimmunoassay (t4), free thyroxine index (ft4i), norepinephrine, epinephrine, prolactin and urinary luteinizing hormone, total estrogens, pregnanediol and total catecholamines. psychologic evaluation and assessment were also done using the mid ... | 1981 | 6119255 |
| phage t4 polynucleotide kinase. | 1981 | 6101053 | |
| in vivo expression of the rii region of bacteriophage t4 present in chimeric plasmids. | the expression of the t4 rii genes in uninfected cells has been examined by use of recombinant plasmids. hybridization analysis of pulse-labelled rna prepared from cells carrying ptb101, a plasmid that contains the end of t4 gene 60 and the beginning of gene riia, shows that about 0.7% of the labelled rna is rii specific. by contrast, only 0.02% of pulse-labelled rna prepared from cells carrying plasmid ptb301, which probably contains the middle-mode rib promoter, may be rii specific. when separ ... | 1981 | 6174845 |
| selective effects of metal ions on rna synthesis rates. | the effects of 14 metal ions (chlorides) on the transcription of calf thymus dna and phage t4 dna with escherichia coli rna polymerase were tested. these assays were conducted under improved conditions of lower ph and in the absence of 2-mercaptoethanol to permit greater stability of the metal ions in solution. among the divalent metal ions tested, the concentration-dependent order of inhibition of overall transcription is pb2+ greater than zn2+ greater than cu2+ greater than be2+ greater than c ... | 1981 | 6175051 |
| norepinephrine and thyrotropin effects on the thyroid in vitro: simultaneous stimulation of iodide organification and antagonism of thyroxine release. | norepinephrine (ne), which has previously been shown to inhibit tsh-induced t4 release by mouse thyroids in vitro, was found to stimulate iodide organification. the concentration of ne (6 x 10(-7) m) necessary to stimulate organification of iodide was 10 times less than the concentration (6 x 10(-6) m) required for inhibition of tsh-induced t4 release. both actions of ne were exerted through an alpha-adrenergic receptor, since they were inhibited by phentolamine but not by l-propranolol. one mil ... | 1981 | 6167436 |
| mithramycin- and 4'-6-diamidino-2-phenylindole (dapi)-dna staining for fluorescence microspectrophotometric measurement of dna in nuclei, plastids, and virus particles. | the properties of two dna-specific fluorochromes, 4'-6-diamidino-2-phenylindole (dapi) and mithramycin, have been analyzed as reagents to quantitate cellular dna by fluorescence microspectrophotometry. optimal staining conditions and concentrations, and the effects of other cellular materials to which the dyes bind, have been evaluated in measurements of the dna of rat, chick, and yeast nuclei, gonyostomum chloroplasts, and t4 particles. use of either fluorochrome permits a high degree of resolu ... | 1981 | 6168681 |
| [determination of serum free thyroxine: normal range, relation to the free thyroxine ratio and total thyroxine (author's transl)]. | the validity of a commercially available f-t4 radioimmunoassay was investigated in 122 patients with different thyroid status. the normal range of f-t4 was 0.60-2.20 ng/dl in euthyroid patients from an endemic goiter area. no significant difference was found between normals and a group taking oral contraceptives (mean = 1.37 ng/dl vs. 1.45 nd/dl). in patients on thyroid hormone or antithyroid drug therapy, f-t4-values showed a better correlation with the results of an oral trh-test than total t4 ... | 1981 | 6169013 |
| dichotomy between serum free triiodothyronine and free thyroxine concentrations in familial thyroxine-binding globulin deficiency. | the mean serum total t4 (3.2 +/- 1.0 micrograms/100 ml) and t3 levels (83 +/- 29 ng/100 ml) in eight euthyroid patients with t4-binding globulin (tbg) deficiency from two families were significantly lower than those in either the unaffected relatives (p less than 0.05) or the control subjects (p less than 0.01). the lowest t4 and t3 values were found in the four hemizygotes (2.4 +/- 0.2 micrograms/100 ml and 69 +/- 25 ng/100 ml, respectively). in contrast to the absolute t4 and t3 levels, the me ... | 1981 | 6169738 |
| triiodothyronine--binding immunoglobulin in a euthyroid patient. | we describe a clinically euthyroid 60-year old woman with a past history of 1311 therapy for treatment of possible toxic nodular goitre. she had an elevated thyroxine level of 188 microgram/l (normal range 50-125 microgram/l) following her therapy, and her tsh was within normal limits at 4.7 mu/l. however her t3 level, as determined by an ria technique employing charcoal to separate bound and free t3, was not measurable. t3 added to the patient's serum could not be recovered, therefore the prese ... | 1981 | 6170477 |
| efficiency of t4 dna ligase-catalyzed end joining after s1 endonuclease treatment on duplex dna containing single-stranded portions. | covalently closed-circular, superhelical sv4o dna was used in all experiments. ecori endonuclease- and hpaii endonuclease-generated unit-length linear duplex dnas were digested with s1 endonuclease under the conditions where single-stranded cna was completely converted into the acid-soluble form. these were subjected to an end-to-end joining test with t4 dna ligase. the ligation efficiency was significantly lower than that of the flush-ended linear duplex dnas which were generated by both hpai e ... | 1981 | 6171303 |
| evaluation of a new free-thyroxin assay. | the amerlex free thyroxin (t4) radioimmunoassay kit (amersham international ltd.) is a new direct equilibrium radioimmunoassay for free t4 based on an antiserum with very high affinity for t4, and a unique 125i-labeled t4 analog as tracer. it is a very simple single-tube radioimmunoassay, making use of amerlex particles to separate antibody-bound from free species. interassay precision (cv) is 3.7% at 13 pmol/l and 2.3% at 30 pmol/l; within-assay precision is 4.2% at 21 pmol/l. the reference int ... | 1981 | 6171364 |
| evoked potentials to auditory probes as indices of cerebral specialization of function--replication and extension. | this study was an attempt to replicate findings from previous work in our laboratory as well as to study the relationship between vertex-referenced and linked mastoid-referenced responses under conditions purported to produce differential hemispheric activation. auditory evoked potentials were recorded between t4-cz, t3-cz, t4-a1a2, and t3-a1a2 to task-irrelevant tone pips superimposed on a baseline condition and on two experimental conditions (verbal and music) hypothesized to differentially ac ... | 1981 | 6171402 |
| immunochemical study of phage t4 lysozyme. | 1981 | 6171456 | |
| value of the determination of "true' free thyroxine in screening the newborn with possible congenital tbg-deficiency for congenital hypothyroidism. | the results of investigation on a family after a screening programme for congenital hypothyroidism are presented. the newborn child and her father appeared to have a congenital thyroxine binding globulin deficiency. problem regarding standard thyroid hormone tests are discussed. it was observed that the free t4 index as well as a commercial free t4 determination introduced earlier can give misleading results. the first experience with another more promising free t4 determination is also presente ... | 1981 | 6172075 |
| [structure and localization of sympathetic neurons in the cat spinal cord]. | by the method of retrograde axonal transport of horseradish peroxidase (hp) structure and localization of sympathetic neurons sending axons to the cranial cervical ganglion (ccg) have been revealed ipsilaterally in the ventral horns and in 4 nuclei of the spinal cord: nucl ilp, nucl. ilf. nucl. ic, nucl. icpe. orientation of the neurons, their number, structure of the nuclei formed by them, degree of the ccg efferentation by the preganglionic fibres, which run from various nuclei, are different. ... | 1981 | 6172095 |
| thyroid hormones effects on rna synthesis in brain and liver of neonatal hypothyroid rats. | the effect of replacement therapy with thyroid hormones upon rna transcription was studied in isolated nuclei of cerebrum and liver from hypothyroid rats. the chronic administration of t4 to hypothyroid rats, from the 5th day of life, during ten days, corrected the rna synthesis in isolated nuclei from cerebrum and liver. the injection of a single dose of t3 to neonatally thyroidectomized rats, ten days after birth, enhanced the endogeneous activity of rna polymerase i and ii of cerebrum and liv ... | 1981 | 6172349 |
| effects of ronnel on growth, endocrine function and blood measurements in steers and rats. | four feeding trials were conducted to determine (1) how the organophosphate ronnel affects thyroid and adrenal circulating hormone levels and blood profile measurements of beef steers and (2) whether the effects of ronnel observed in beef steers could be produced in growing rats. in trial 1, four groups of eight steers each were given either no ronnel or 4 mg ronnel/kg body weight daily in diets fed at either limited (1.8% of body weight) or ad libitum intake. after 7 weeks, intake levels, but n ... | 1981 | 6172413 |
| processing of bacteriophage t4 trnas. the role of rnaase iii. | 1981 | 6175760 | |
| subpopulations of the t4+ inducer t cell subset in man: evidence for an amplifier population preferentially expressing ia antigen upon activation. | prior studies demonstrated that ia molecules were expressed on a fraction of human peripheral t4+ inducer cells upon stimulation by soluble antigen. in the present study, we utilized a fluorescence-activated cell sorter to separate antigen-activated t4+,ia+ and t4+,ia- populations and characterized their function. it was found that the t4+,ia+ population contained the majority of proliferating t cells as assessed by tritiated thymidine incorporation. this proliferation largely appeared to be non ... | 1981 | 6161168 |
| the relationship of androgen to the thyrotropin and prolactin responses to thyrotropin-releasing hormone in hypogonadal and normal men. | fluoxymesterone, an androgen not converted to estrogen, caused a significant decrease in the tsh response to trh in 11 men with primary hypogonadism [maximum change in tsh: before treatment, 11.3 +/- microu/ml (mean +/- se); 8.9 +/- 1.0 after 2 weeks (p less than 0.001); 8.2 +/- 1.1 after 6 weeks (p less than 0.01)]. there was a significant fall in serum t4-binding globulin (tbg) (measured directly by ria) without a change in the free t4 or free t3 index. fluoxymesterone had no effect on the prl ... | 1981 | 6161941 |
| effect of neonatal thyroid deficiency on the catecholamine, substance p, and thyrotropin-releasing hormone contents of discrete rat brain nuclei. | the effects of neonatal thyroidectomy and thyroid hormone replacement therapy on the development of catecholamine-, trh-, and substance p-containing neurons in discrete rat brain nuclei were studied. newborn male rats were rendered hypothyroid by the injection of 125 muci 131i and, after 45 days, were compared with normal littermate controls and 131i-injected animals subsequently maintained on t4 injections. the peptide or catecholamine content of discrete brain nuclei removed by punches of froz ... | 1981 | 6164545 |
| free thyroxine estimates in nonthyroidal illness: comparison of eight methods. | eight methods for estimating free serum t4 were compared for 26 patients with nonthyroidal illness (nti) and 16 hypothyroid patients with comparable total t4 (tt4) concentrations. free t4 values were determined by equilibrium dialysis, enzyme immunoassay (abbott), antibody-coated tube (clinical assays), antibody-coated microfine silica (corning immunophase), microencapsulated antibody (damon), and free t4 index using the t3 uptake ratio or thyroxine-binding globulin method. equilibrium dialysis, ... | 1981 | 6164687 |
| composition and efficacy of iodothyronine immunogens and host responsiveness in radioimmunoassay (ria) for serum thyroxine (t4). | 1981 | 6165611 | |
| uptake, metabolism and action of triiodothyronine in calf-thyroid slices. | t3 and t4 at 10(-6)m caused a significant inhibition on [3h]uridine incorporation into rna in calf-thyroid slices. this effect was not altered by addition of 10(-3) m ptu or mmi. the metabolism of 125i-t3 was studied under the same conditions. there was a very slight dehalogenation after 60 min (less than 5%) which was inhibited by ptu. the time course of the uptake of labeled t3 showed a temperature dependence, and this uptake was not altered by 10(-6)m ki, 1-t4, dit or mit, thus indicating spe ... | 1981 | 6165633 |
| thyroid activity during hepatocarcinogenesis by n-2-fluorenylacetamide. | during chemical carcinogenesis by n-2-fluorenylacetamide, the hormonal status of female sprague dawley rats is largely modified. thyroids present a modified activity which is evidenced by the histological study as well as t4 and t3 assays. in hypophysis, thyrotropic cells were found in an hyperactive state, a fact which is in agreement with the changes observed in thyroid follicles size and epithelial cells demonstrating an hyperactivity of thyroid glands. despite this histological result, t4 se ... | 1981 | 6165727 |
| protein-linked iodotyrosines in serum after topical application of povidone-iodine (betadine). | markedly elevated serum pbi levels occur after therapy with povidone-iodine (betadine), an iodine-polyvinylpyrrolidone iodophor. in this study, we investigated the serum iodine compounds from a severely burned patient with normal initial thyroid function tests who was swabbed with betadine ointment and received daily therapeutic baths in betadine. three and 9 days after therapy, his serum contained 93 and 168 micrograms pgi/dl, respectively (normal range, 4-8), while the serum t4 and free t4 ind ... | 1981 | 6165730 |
| serum ratio of triiodothyronine to thyroxine, and thyroxine-binding globulin and calcitonin concentrations in graves' disease and destruction-induced thyrotoxicosis. | the serum ratios of t3 to t4, and t4-binding globulin (tbg) and calcitonin concentrations were studied in cases of thyrotoxic graves' disease and destruction-induced thyrotoxicosis. in 272 patients with graves' disease, 209 of 240 (87%) untreated patients without complications had high t3 to t4 ratios (nanograms per micrograms) of more than 20. six of 32 (19%) patients with graves' disease who had complications (15 with pregnancy, 14 with increased tbg, and 3 with conditions associated with a lo ... | 1981 | 6165731 |
| pharmacological interference with in vitro tests of thyroid function. | numerous drugs may cause changes in the serum concentrations of t4 and of t3. if such alterations are not recognized an incorrect diagnosis may result. in moderate degrees of hypo- and hyperthyroidism thyroid hormone levels may be spuriously normal, or the influence of pharmacological substances may lead to false diagnosis of thyroid disease in euthyroid patients. since prediction of such alterations remains uncertain, it may be necessary to perform additional investigations when a potential art ... | 1981 | 6165878 |
| sites of termination of in vitro dna synthesis on ultraviolet- and n-acetylaminofluorene-treated phi x174 templates by prokaryotic and eukaryotic dna polymerases. | in vitro dna synthesis on a phi x174 template primed with a restriction fragment and catalyzed by the escherichia coli dna polymerase i large (klenow) fragment (pol i) terminates at the nucleotide preceding a site that has been altered by ultraviolet irradiation or treatment with n-acetylaminofluorene. termination on ultraviolet-irradiated templates is similar when synthesis is catalyzed by e. coli dna polymerase iii holoenzyme (pol iii), phage t4 dna polymerase, a polymerase alpha from human ly ... | 1981 | 6165985 |
| effects of thyroid hormones, antithyroid drugs and iodide on in vitro conversion of thyroxine to triiodothyronine. | in an attempt to study the effects of thyroid hormones, antithyroid drugs and iodide on the conversion of thyroxine (t4) to triiodothyronine (t3), the liver was obtained from normal, hypothyroid and hyperthyroid rats treated with t4 or t3 . t4 was incubated with liver homogenates and generated t3 was measured. 1. the conversion of t4 to t3 was less in the liver homogenates from thyroidectomized, methylmercaptoimidazole (mmi)-treated and propylthiouracil (ptu)-treated rats. the magnitude of decre ... | 1981 | 6166420 |
| familial euthyroid thyroxine excess: an appropriate response to abnormal thyroxine binding associated with albumin. | responses of the pituitary-thyroid axis and t4 binding to plasma proteins were studied in three kindreds with familial euthyroid t4 excess, an autosomal dominant condition in which affected subjects have high concentrations of plasma t4 with a high free t4 index, but normal free t4 by equilibrium dialysis. treatment of affected subjects with exogenous t4 or t3 led to gradual suppression of tsh secretion when the free level of t4 or t3 increased above normal. when total t4 was reduced toward norm ... | 1981 | 6166631 |
| polyamines, hydrolases, (pap, lap, sdh, plasmin) tsh, t3, t4 and c-peptide in benign hyperplasia of the prostate. | high concentrations of polyamines have been found in the normal human prostate. the profile of these amines appeared significantly changed in benign hypertrophy of the prostate. an increase of spermine and a fall of putrescine were always found in patients with a hypertrophied prostate weighing more than 30 g. alterations of plasmin in these tissues seemed to reflect changes in the matrix; abnormalities of thyroid and pancreatic function documented by changes in the serum levels of tsh and c-pep ... | 1981 | 6167047 |
| ratio of thyroxine to thyroxine-binding globulin. an assessment of the validity of a single reference range. | the thyroxine:thyroxine-binding globulin ratio in serum (t4:tbg) has been proposed as a measure of thyroid status unaffected by altered binding protein levels. here 320 apparently euthyroid patients are used to derive euthyroid ranges for serum thyroxine concentrations at specific tbg levels. these ranges are compared with those predicted by a t4:tbg reference range derived from the same patient data. the comparison suggests that the ratio would give false positives for hyperthyroidism at low tb ... | 1981 | 6167196 |
| growth retardation in fetal alcohol syndrome. unresponsiveness to growth-promoting hormones. | the relationships between growth retardation and metabolic and hormonal parameters were studied in 7 children with fetal alcohol syndrome (fas). fasting blood concentrations of tsh, t4, t3, fsh and lh were normal. plasma prolactin concentrations after chlorpromazine stimulation were normal. 3 children had abnormal oral glucose tolerance tests with increased plasma insulin response. peak plasma growth hormone responses to insulin-induced hypoglycemia were elevated in 5 patients. fasting bioassaya ... | 1981 | 7201377 |
| thyroid function in parkinson disease. | we conducted a prospective study of thyroid function in 46 patients with parkinson disease and 46 age- and sex-matched controls with other neurologic disease. there was no statistical difference in serum thyroxine (t4) and t3 resin uptake (t3u) between the two groups. neither the duration nor the quantity of l-dopa or carbidopa/l-dopa (sinemet) therapy influenced these assessments of thyroid function. however, 3 of 46 parkinson patients were hypothyroid, whereas none of 46 controls was hypothyro ... | 1981 | 7192833 |
| a study of long acting propranolol in the early management of hyperthyroidism. | 1 the effects of propranolol in its long acting formulation, 160 mg long acting (la) propranolol, were assessed in a double-blind, controlled study of 20 hyperthyroid patients. further observations with the addition of carbimazole were also made. 2 la propranolol effectively improved the clinical manifestations of hyperthyroidism. 3 plasma concentrations of total t4 and rt3 were increased, but total t3 did not fall significantly during la propranolol treatment. 4 la propranolol with carbimazole ... | 1981 | 7041935 |
| characteristics of succinylcholine-produced phase ii neuromuscular block during enflurane, halothane, and fentanyl anesthesia. | the characteristics of phase ii neuromuscular block following repeated intravenous injections of succinylcholine (sch) were determined in 15 adult patients during enflurane, halothane, or fentanyl-nitrous oxide anesthesia. the onset of phase ii block (train-of-four ratio (t4) equal to 0.5) occurred following a cumulative sch dose of 4.4 +/- 0.3 (sem) mg/kg of enflurane, 5.1 +/- 0.5 mg/kg of halothane, or 6.4 +/- 0.5 mg/kg of fentanyl. the cumulative sch dose producing phase ii block during fenta ... | 1981 | 7193996 |
| in vitro binding of triiodothyronine to rat liver mitochondria. | saturable high affinity t3 binding sites were detected in a mitochondrial fraction enriched in internal membranes and partly solubilized by triton x-100. specific t3 binding to the solubilized sites, only detected at low t3 concentrations, was optimal at ph 8.0 and not dependent upon the presence of divalent cations or reducing agents; it was destroyed by heat and proteolytic enzymes. the solubilized t3 binding sites were distributed, after sephadex g-200 gel filtration, between two peaks of sim ... | 1981 | 7195560 |
| the interrelationship of thyroid hormones with vitamin a and zinc nutritional status in patients with chronic hepatic and gastrointestinal disorders. | to define the role of vitamin a, retinol binding protein, and zinc deficiency in producing the thyroid hormone abnormalities found in chronic illness, we studied 62 clinically stable patients with hepatic and gastrointestinal disorders. serum triiodothyronine (t3) and free t3 index (ft3i) were depressed compared to controls (p less than 0.05) in the patients. retinol binding protein and prealbumin levels correlated with both t3 and ft3i (p less than 0.01), whereas vitamin a levels did not. vitam ... | 1981 | 7196691 |
| [metabolic evaluation and etiology of "preclinical hypothyroidism": a preliminary report]. | the trh test, using synthetic trh (tsh-releasing hormone) is the most sensitive test for the assessment of thyroid function. it may show elevated basal tsh and/or an exaggerated tsh response to trh, despite normal thyroid hormone levels (t4, ft4i, t3). this condition is termed "preclinical hypothyroidism" (pc hypo). thyroid hormone levels, the clinical index of billewicz and metabolic impact on target tissues were studied prospectively in 38 pc hypo women and compared with 20 controls matched fo ... | 1981 | 7197052 |
| circulating reverse triiodothyronine in humans during exercise. | circulating thyroxine (t4), triiodothyronine (t3) and reverse triiodothyronine (rt3) as well as blood lactate and glucose concentrations were measured in a group of 12 trained volunteer subjects prior to and after swimming 0.18 or 0.9 km, to determine if increase in metabolic activity was accompanied by diversion of t4 monodeiodination from the active (t4 to t3) to the inactive (t4 to rt3) pathway. the resting t4, t3, and rt3 levels were 8.5 micrograms . 100 ml-1, 108 ng . 100 ml-1, and 57 ng . ... | 1981 | 7198038 |
| [effect of the ingestion of soybean fractions, raw or autoclaved, on the rat thyroid]. | feeding rats for 16 days a water soluble fraction from raw soy flour caused a 40% enlargement of thyroid weight, a similar decrease of 131i uptake and an increase of tri-iodo-thyronine-binding capacity of rat serum. this effect was heat labile. the same fraction autoclaved was able to reduce radioactive iodine uptake and to decrease t3-binding capacity. in longer assays (29 days) this same fraction produced a 30% increase of 131i uptake and altered thyroid hormones increasing t3 and t4 synthesis ... | 1981 | 7199898 |
| thyroid hormones and lipolysis in physically trained rats. | in rats a single bout of exercise resulted in increased triiodothyronine (t3), thyroxine (t4), and triiodothyronine/reverse triiodothyronine (t3/rt3) ratio 20 hr after exercise. the effect of norepinephrine on lipolysis in vitro was potentiated. in trained rats no changes were found in t4, t3, or rt3 concentrations. the t3/rt3 ratio as well as basal and stimulated tsh concentrations decreased in comparison with sedentary, freely eating rats. moderate food restriction to produce a body weight sim ... | 1981 | 7207199 |
| predominant t3 synthesis in the metastatic thyroid carcinoma in a patient with t3-toxicosis. | a metastatic mixed papillary and follicular thyroid carcinoma synthesizing predominantly triiodothyronine (t3) producing thyrotoxicosis is described. there was a concentration of 131i in numerous masses on the lung as well as a mass in the neck. bmr was +38%. serum t4, t3-ru and t3 were 6.6 microgram/100 ml, 35.1% and 9.0 ng/ml, respectively. serum tsh was not detectable and was not increased by the injection of 500 microgram of synthetic trh. decompressive surgery, performed while the patient w ... | 1981 | 7207204 |
| repair of ultraviolet radiation damage in xeroderma pigmentosum cells belonging to complementation group f. | dna-repair characteristics of xeroderma pigmentosum belonging to complementation group f were investigated. the cells exhibited an intermediate level of repair as measured in terms of (1) disappearance of t4 endonuclease-v-susceptible sites from dna, (2) formation of ultraviolet-induced strand breaks in dna, and (3) ultraviolet-induced unscheduled dna synthesis during post-irradiation incubation. the impaired ability of xp3yo to perform unscheduled dna synthesis was restored, to half the normal ... | 1981 | 7207491 |
| common precursor of lysozymes of hen egg-white and bacteriophage t4. | the lysozymes of hen egg-white and bacteriophage t4 have similar catalytic properties but their amino acid sequences are not homologous. the question therefore arises whether they are derived from a common ancestral protein or have arisen independently. on the basis of the data we have gathered, it is shown here that the two enzymes are similar in the conformation of their backbones, in their modes of binding substrates, in specific protein--substrate interactions and in their presumed modes of ... | 1981 | 7207627 |
| action of human growth hormone (hgh) on extrathyroidal conversion of thyroxine (t4) to triiodothyronine (t3) in children with hypopituitarism. | to study the action of human growth hormone (hgh) on peripheral metabolism of serum thyroxine (t4), an oral loading dose of levothyroxine (1.2 mg/m2) was administered to seven children with hypopituitarism before initiation of hgh therapy. serum concentrations of triiodothyronine (t3), t4, reverse triiodothyronine (rt3), and thyroxine-binding globulin (tbg) capacity were measured sequentially for 6 days. the study was repeated after 4 wk of treatment with hgh. serum concentrations of t4 were not ... | 1981 | 7208169 |
| regulation of tsh secretion and thyroid function in cushing's disease. | in 15 untreated patients with cushing's disease the regulation of tsh secretion and thyroid function was evaluated. the maximal increment of plasma tsh to trh was 3.8 +/- 2.2 vs 8.7 +/- 2.9 muu/ml (patients with cushing's disease vs controls; mean +/- sd; p less than 0.001). free thyroxine-index was 21.8 +/- 5.2 vs 27.7 +/- 5.0 (p less than 0.001). plasma t3 1.45 +/- 0.24 vs 1.96 +/- 0.32 nmol/l (p less than 0.001) and reverse t3 0.22 +/- 0.05 vs 0.20 +/- 0.08 nmol/l (n.s.). the maximal tsh incr ... | 1981 | 7211104 |
| influence of smoking on thyroid activity. | serum concentrations of t4, t3, rt3 and tsh were determined before and after cessation of chronic smoking in healthy subjects. their body weights were measured at the same intervals. abstention from smoking was monitored through determination of co content in blood. cessation of smoking was accompanied by small reductions in serum t4 and rt3 and by a small rise in serum tsh. serum t3 was not significantly altered. a minor rise in body weight was noticed. it is suggested that smoking promotes a m ... | 1981 | 7211488 |
| feedback regulation of thyrotropin by thyroxine under physiological conditions. | we have studied the contribution of thyroxine (t4) itself to the feedback regulation of thyrotropin (tsh) secretion. thyroidectomized rats received t4 replacement by one of two methods: 1) 5 or 10 micrograms t4 . 100 g-1 . day-1 in drinking water or 2) continuous subcutaneous infusion of 0.5, 1.0, or 2.0 micrograms t4 . 100 g-1 . day-1. replacement of 5 micrograms t4 . 100 g-1 . day-1 resulted in elevated plasma t4 and tsh while triiodothyronine (t3) was decreased. replacement of 10 micrograms t ... | 1981 | 7212062 |
| kinetics of formation of deoxyribonucleic acid cross-links by 4'-(aminomethyl)-4,5',8-trimethylpsoralen. | if a mixture of t4 deoxyribonucleic acid (dna) and 4'-(aminomethyl)-4,5',8-trimethylpsoralen is irradiated with two closely spaced pulses of long-wave uv laser light, the resulting cross-linking is dependent on the time delay between the pulses. as the delay lengthens to 1 mus, a rise in the number of cross-links is observed which follows first-order kinetics. this delay, the time required for most monoadducts to be able to absorb a second photon and thereupon form a cross-link, is interpreted i ... | 1981 | 7213608 |
| [changes in the concentration of triiodothyronine and thyroxine in rabbit fetuses following encephalectomy]. | triiodothyronine (t3) and thyroxin (t4) were detected by radioimmunoassay in the thyroid gland and serum of encephalectomized and intact rabbit fetuses. encephalectomy performed on the 22-23d day of development did not produce any effect on thyroid hormone concentrations in 29-day-old fetuses. however, on the 30th day the concentrations of t2 and t4 in the thyroid gland were significantly lower in the encephalectomized fetuses than in the intact ones. these findings were consistent with changes ... | 1981 | 7214013 |
| [a study of thyroid function in patients with psychoaffective disease (author's transl)]. | the purpose of the present study was to investigate dysfunctions in thyroid gland secretion and regulation among patients with manic-depressive psychosis. plasma levels of thyroxine (t4), triiodothyronine (t3) and thyroid stimulating hormone (tsh) were measured by radioimmune assays, and tsh responsiveness (delta tsh) to synthetic tsh-releasing hormone (trh) 250 microgram iv was determined in 20 untreated patients, 19 patients under neuroleptics and 19 patients under antidepressants. untreated p ... | 1981 | 7220333 |
| in vitro transcription of bacteriophage t4 trna gene cluster from two different promoters. | analysis of primary transcripts made by escherichia coli rna polymerase on t4 dna containing an intact or partially deleted trna gene cluster demonstrates that the t4 trna genes are transcribed from two promoters differing in their strength. the stronger (p1) and the weaker (p2) promoters are located at distances of 1 kb and 1.5 kb from the trna genes, respectively. selective initiation of individual transcripts with dinucleotides shows that p1 and p2 promoters contain the sequences tat and cac ... | 1981 | 7220346 |
| [lethal and mutagenic action of electron irradiation in a range of low temperatures (-196 degrees c) on the rii mutant of t4 phage]. | 1981 | 7220821 | |
| [endocrinologic findings in crohn's disease]. | in 45 patients with radiologically and endoscopically/histologically proven crohn's disease the partial functions of the anterior pituitary gland were measured prior to steroid therapy. no deficiencies were observed with respect to cortico-, thyro-, gonado-, and somatotropic functions. in 25 out of 40 patients (63%) the typical diurnal rhythm of cortisol secretion was absent when assessed by a distinctly elevated 6 p.m. cortisol serum level. in about one fourth of 22 patients t3-ria was reduced, ... | 1981 | 7221527 |
| thyroid hormone-catecholamine interrelationships during exposure to cold. | basal plasma levels for adrenalin (a), noradrenalin (na), l-triiodothyronine (t3), and l-thyroxine (t4) were determined in rats with a chronically inserted catheter. the experiments described in this report were started 3 days after the surgical procedure when t3 and t4 levels had returned to normal. basal levels for the catecholamines were reached already 4 h after the operation. the t3/t4 ratio in plasma was significantly increased after 3, 7, and 14 days in rats kept at 4 degrees c and the sa ... | 1981 | 7223318 |
| hormone responsiveness of a transplantable rat chondrosarcoma: iii. ultrastructural evidence of in vivo hormone dependence. | the effects of growth hormone (gh) and/or cortisone and thyroxine (t4) treatment on the ultrastructure of the transplantable swarm rat chondrosarcoma grown in hypophysectomized rats were studied. marked atrophy of the tumor occurred when it was grown in hypophysectomized rats. maximal restoration of tumor growth in hypophysectomized rats was achieved when cortisone, t4, and gh were administered in combination. an intermediate degree of tumor growth restoration was observed when tumor-bearing hyp ... | 1981 | 7223863 |
| dependence of the auditory brainstem response on electrode location. | the human auditory brainstem electric response to right ear stimulation was studied for effects dependent on active electrode location. recordings were made with the reference electrode located on the sternum and the ground electrode located on the nasion. responses showed significant differences for electrodes that were located at two cephalic (left and right mastoid processes) and three non-cephalic (wrist and left and right sides of the neck) active locations. components 1 to 6 (jewett vertex ... | 1981 | 7224967 |
| postoperative changes in coagulation and fibrinolysis independent of neurogenic stimuli and adrenal hormones. | postoperative changes in various haemostatic parameters (capillary bleeding time, platelet count, fibrinogen, fibrinmonomers, prothrombin, antithrombin iii, factor viii procoagulant, factor viii antigen, euglobulin clot lysis time, streptokinase lysis time, fibrinogen related antigens, alpha 1-antitrypsin and alpha 2-macroglobulin) plasma glucose and cortisol were studied in 12 female patients undergoing elective abdominal hysterectomy during either general anaesthesia or epidural analgesia (t4- ... | 1981 | 7225737 |
| on the role of nadph and glutathione in the catalytic mechanism of hepatic thyroxine 5'-deiodination. | a possible metabolic linkage between hepatic thyroxine (t4) 5'-deiodination and the nadph-glutathione (gsh) cycle was studied in rat liver. supplementation of 1 mm nadph to stocked liver homogenates in vitro produced 4 fold increase in 3, 5, 3'-triiodothyronine (t3) formation from t4, whereas the effect of 1 mm fmn, fdn, nad, nadh, or gsh was relatively small. an exponential dose-response relation was obtained between nadph and t3 generated. the dose-dependent increase in t3 formation on gsh was ... | 1981 | 7052928 |
| reciprocal relation between serum thyrotropin levels and intrapituitary 3,5,3'l-triiodothyronine generating activity from thyroxine in perinatal rats. | thyroxine (t4) 5'-monodeiodinating activity in rat pituitary was studied, using paper chromatography. rat anterior pituitary homogenates were incubated with 125i-t4, dithiothreitol and unlabeled l-t4 at 37 degrees c. the incubation mixture was extracted by ethanol and the extracts were subjected to descending paper chromatography. the conversion ratio of t4 to 3, 5, 3'l-triodothyronine (t3) was calculated from the radioactivity of t3 spot to total radioactivity on the paper strip. the t3 amount ... | 1981 | 7052929 |
| [haptoglobin type, serum concentration of haptoglobin and agglutinating titre against t4-antigen of streptococcus pyogenes in erysipelas (author's transl)]. | 1981 | 7049773 | |
| polyamine biosynthesis in escherichia coli: construction of polyamine-deficient mutants. | previous work is summarized on the biosynthetic pathway for polyamines in escherichia coli. deletion mutants have been obtained in the various biosynthetic steps, resulting in cells with no polyamines. these mutants grow at one-third the rate of polyamine-supplemented cultures and can serve as suitable hosts for bacteriophages t4, t7, q beta, and f2. the major effects of polyamine deficiency in these polyamine-deficient strains are: (i) these cells do not serve as hosts for bacteriophage gamma a ... | 1981 | 7040834 |
| on the role of the escherichia coli rna polymerase sigma factor in t4 phage development. | the rpod800 mutation causing the temperature sensitivity of escherichia coli rna polymerase sigma factor has been used to demonstrate that the bacterial sigma factor is necessary throughout t4 phage development. in t4-infected rpod800 mutant cells rna synthesis is equally depressed at restrictive temperature at early and late stages of infection. the results show the bacterial sigma factor to be necessary for the synthesis of all rna types in infected cells. | 1981 | 7038380 |
| assembly of bacteriophage t4 tail fibers: identification and characterization of the nonstructural protein gp57. | formation of both the tail fiber and the baseplate of bacteriophage t4 depends on the product of t4 gene 57. a single amber mutation in that gene causes loss of two t4-specific proteins. their molecular weights are 18,000 and about 6,000, respectively, based on their electrophoretic mobilities in sds-polyacrylamide gels. e. coli carrying a cloned t4 dna fragment of about 700 basepairs, which directs the synthesis of the smaller protein only, specifically supports the growth of gene 57 amber muta ... | 1981 | 7038383 |
| [typing of e. coli rough strains with phages (author's transl)]. | two phage-sets were used for the typing of 408 e. coli rough strains isolated from patients with urinary tract infections, using the 13 phages a to m (11), we were able to type 225 (55.1 per cent) strains. 143 (35 per cent) of these strains were found to have k antigen, 84 different phage patterns could be recognized. phage patterns ghk, m, f, and am were the most common ones found. the application of the typical rough phages br10, c21, 6sr, fp1, t4, and the felix o (fo) phage led to the success ... | 1981 | 7036596 |
| diurnal hormone-metabolite profiles in hypothyroidism. | to investigate the influence of thyroid hormones on intermediary metabolism in man, hormone and metabolite profiles were obtained over a 12-h period of normal meals and activity in eight hypothyroid subjects before and during thyroxine replacement therapy, and in sixteen matched controls. the fasting blood glucose concentration and the mean 12-h blood glucose concentration were normal in hypothyroid subjects but the blood glucose response to breakfast was exaggerated. fasting blood lactate and p ... | 1981 | 7035015 |
| t4 head assembly and high temperature. | 1981 | 7036182 | |
| bacteriophage-host interactions in assembly. | because of their small genome size and their parasitic way of life, bacteriophages have obligatorily evolved in such a way as to efficiently utilize many of the bacterial functions necessary for dna replication, transcription, translation, and morphogenesis. in many instances the phage and host functions act in parallel in these processes, e.g. t4 and host dna ligase. the specific nature of the host's contribution to phage morphogenesis is being pursued by the studies summarized here. the isolat ... | 1981 | 7036183 |
| the effects of altered thyroid status on lipid metabolism in the genetic hyperlipemic zucker rat. | exposure to thyroid hormone (t4) has been known to affect the plasma triglyceride (tg) as well as the plasma cholesterol level, but the mechanisms and degree of response in genetic hyperlipidemic states have not been defined. in the present study, we examined tg secretion and removal in vivo in genetically hyperlipemic zucker rats maintained in hypothyroid, euthyroid, and hyperthyroid states for 6 weeks. the induction of the hypothyroid state resulted in marked weight loss with reduced food inta ... | 1981 | 7008806 |
| [measurement of intrathyroidal iodine content by x fluorescence: usefulness and application (author's transl)]. | x fluorescence quantification of thyroid gland iodine content was performed in 113 subjects. euthyroid patients averaged 15.6 +/- 4.8 mg in the absence of goiter (18 subjects) and 23.7 +/- 11 mg when the gland was goitrous (11 subjects p less than 0.05). in grave's disease (28 patients), thyroid iodine content increased significantly (21.7 +/- 11 mg, p less than 0.05. no correlation was found between this parameter and thyroid hormone blood level. during the treatment by carbimazole, patients ca ... | 1981 | 7010274 |
| regulation of the synthesis of the t4 dna polymerase (gene 43). | 1981 | 7010774 | |
| immune response in the bovine mammary gland after intestinal, local, and systemic immunization. | the immune response in mammary glands of cattle was measured after intestinal, local, and systemic immunization with t4 bacteriophage. nonlactating pregnant cows were immunized by infusions into the intestine or mammary gland and by subcutaneous injections in the region of the prescapular or external inguinal lymph nodes. titers of antibodies of different isotypes were measured in serum and in lacteal secretions by enzyme-linked immunosorbent assay, and numbers of cells producing antibodies of e ... | 1981 | 7012016 |
| portal vein blood insulin and glucagon are increased in experimental hyperthyroidism. | decreased glucose tolerance in hyperthyroidism has been long recognized; however, the mechanism(s) responsible for altered carbohydrate metabolism have not been elucidated. studies of insulin secretion in hyperthyroidism have been reported by several investigators; however, the results are not conclusive since hormone secretion has been reported as increased, normal, or decreased. few investigations of glucagon secretion in hyperthyroidism have been described; thus, the role of the alpha cell ho ... | 1981 | 7014195 |
| the effect of a high calory diet on hormonal changes in young men during prolonged physical strain and sleep deprivation. | major changes occur in the serum level of several hormones during 5 days of heavy and continuous physical activities, with less than a total of 2 h of sleep. the present investigation was designed to evaluate the importance of caloric deficiency, energy requirement being about 8,000-10,000 kcal/24 h. a comparison between well fed subjects and those with food deprivation revealed significantly higher levels of (t3) triiodothyronin, insulin and thyroid stimulating hormone (tsh) in the well-fed sub ... | 1981 | 7014216 |
| alterations in serum concentrations of t4, t3 and r-t3 in normoglycemic glucose clamp-evidence of intercompartmental shifting ? | 1981 | 7014406 | |
| identification of a second escherichia coli groe gene whose product is necessary for bacteriophage morphogenesis. | previous work has uncovered the existence of an escherichia coli locus, groe, that is essential for bacterial growth, lambda phage and t4 phage head morphogenesis, and t5 phage tail assembly. our genetic and biochemical analyses of lambda groe+ transducing phages and their deletion and point mutant derivatives show that the groe locus consists of two closely linked genes. one groe gene, groel, has been shown to encode the synthesis of a 65,000 mr polypeptide, whereas the second, groes, codes for ... | 1981 | 7015340 |
| variants of a cloned synthetic lactose operator. i. a palindromic dimer lactose operator derived from one stand of the cloned 40-base pair operator. | starting with one strand of the 40-bp synthetic operator (sadler et al., 1978), we have constructed and cloned a 66-bp, palindromic dna segment with the following sequence (formula: see text), where the horizontal arrows indicate the locations of the two 21-bp "core" operator sequences in this segment and the vertical arrow designates the dyad axis of symmetry. upon denaturation and rapid renaturation, each strand of this fragment forms a hairpin molecule still retaining an ecori cohesive end. t ... | 1981 | 7016667 |
| interaction of streptococcal cell wall components with fibrinogen. i. adsorption of fibrinogen by immobilized t-proteins of streptococcus pyogenes. | immobilized streptococcal t1-, t3- and t4-proteins to ah-sepharose 4b were tested for their ability to absorb human fibrinogen. purified fibrinogen and plasma samples were used for affinity chromatography. t1-protein was able to retain specifically fibrinogen from plasma. t4-protein bound fibrinogen in a similar manner, but it was not as specific as t1-protein. t3-protein failed to bind purified fibrinogen as well as fibrinogen from plasma. adsorption of fibrinogen was accomplished using a 0.05 ... | 1981 | 7016738 |
| replication of phase fd rf with fd gene 2 protein and phage t4 enzymes. | bacteriophage fd replicative form dna with a nick in the viral strand serves as a template for dna replication with purified bacteriophage t4 enzymes. as anticipated from previous in vitro studies carried out with this system (morris, c. f., sinha, n. k., and alberts, b. m. (1975) proc. natl. acad. sci. u.s.a. 72, 4800-4804), dna is synthesized by a rolling circle mechanism. we show here that the dna strands synthesized are processed by the phage fd gene 2 protein into unit length products, prov ... | 1981 | 7016862 |
| membrane potential changes during the first steps of coliphage infection. | immediately after adsorption, phages t4 and t5 induce a partial depolarization of the host cytoplasmic membrane. infected bacteria respond to this phage-induced effect by a repolarization that leads to a new steady state of reduced membrane potential. the rate and extent of repolarization are adjusted to the intensity of depolarization, which depends on the number of adsorbed phages. consequently, the new steady state membrane potential is attained in the same interval of time regardless of the ... | 1981 | 7017710 |
| the mechanism of impaired t3 production from t4 in diabetes. | 1981 | 7018973 | |
| [combination treatment of localised breast cancers with a high risk of metastatic disease. the results of a prospective controlled study (author's transl)]. | 74 patients who had adenocarcinoma of the breast that was inoperable at the beginning because of local extension but without inflammation and without diagnosable metastases (t3b, t4, n1, 2 or 3, mo) were submitted to a therapeutic regime which consisted consecutively of: 1) preliminary chemotherapy, 2) radiotherapy and/or extended radical surgery when there was any residual tumour, and 3) follow-up adjuvant chemotherapy. two types of combinations of cytostatic drugs were used: adriamycin, vincri ... | 1981 | 7019300 |