Publications

mechanism of pbr322 transduction mediated by cytosine-substituting t4 bacteriophage.	a cytosine-substitution type mutant of bacteriophage t4 (t4dc phage) has been shown to mediate the transfer of plasmid pbr322. the transduction frequency was around 10(-2) per singly infected cell at low multiplicity of infection. the transductants contained either a monomer or multimers of pbr322. the transducing capacity of t4dc phage was resistant to methylmethanesulfonate treatment. the results of southern blotting experiments have indicated that the pbr322 dna exists as head-to-tail concate ...	1982	6752658
thyroid dysfunction among long-term survivors of bone marrow transplantation.	thyroid function studies were followed serially in 27 long-term survivors (median 33 months) of bone marrow transplantation. there were 15 men and 12 women (median age 13 1/12 years, range 11/12 to 22 6/12 years). aplastic anemia (14 patients) and acute nonlymphocytic leukemia (eight patients) were the major reasons for bone marrow transplantation. pretransplant conditioning consisted of single-dose irradiation combined with high-dose, short-term chemotherapy in 23 patients, while four patients ...	1982	6753576
pyrimidine dimer-dna glycosylases: studies on bacteriophage t4-infected and on uninfected escherichia coli.	pyrimidine dimer (pd)-dna glycosylase activity has been reported in both the m. luteus and phage t4 uv endonucleases. in the present studies the t4 pd-dna glycosylase has been purified close to physical homogeneity using an assay that measures the release of free thymine from uv-irradiated poly ([h5] dt):poly (da), after the photo-reversal of thymine-thymine dimers. the activity has also been demonstrated in vivo following infection of uv-irradiated e. coli uvr- cells with phage t4. under these ...	1982	6753948
primary structure of parvalbumin from rat skeletal muscle.	the primary structure of parvalbumin from rat skeletal muscle has been determined principally by automated sequencing of tryptic peptides using 4-n,n-dimethylaminoazobenzene 4'-isothiocyanate as the edman reagent on a solid-phase sequencer. remaining positions and most peptide overlaps were identified by analysis of peptides arising from cnbr, chymotryptic and staphylococcus aureus protease cleavages and through digestions with carboxypeptidases a, b and y. reverse-phase high-performance liquid ...	1982	6754379
placebo-controlled cross-over study of effects of org od 14 in menopausal women.	a double-blind cross-over study with org od 14 and placebo was performed in 82 menopausal patients presenting with hot flushes and associated symptoms. patients were randomly allocated to org od 14 or placebo as first treatment, and switched to placebo or org od 14 as second treatment. each treatment period lasted for 16 weeks; no wash-out period was introduced. tablets containing 2.5 mg of org od 14 or matched placebo tablets were supplied. data on the following variables were obtained and anal ...	1982	6754512
hormonal control of casein synthesis in organ culture of the bovine lactating mammary gland.	explants from lactating bovine mammary gland were cultured in vitro in serum-free medium though 1-9 d. casein synthesis was determined by [32p] incorporation into newly synthesized ca rennin precipitable fraction. high correlation (r = 0.98) was found between incorporation of [32p] and [3h]amino acids in explants cultured under different hormonal regimes, thus indicating that post-translational phosphorylation is not a rate-limiting step in casein synthesis. hormonal effects on casein synthesis ...	1982	6754783
evaluation of fluorescence polarization immunoassay for serum thyroxine determination.	we have evaluated a fluorescence polarization immunoassay for thyroxine (t4) using the polarization analyzer ibf-129. fluorescence polarization could be measured within 20 to 23 seconds after addition of fluorescein labeled thyroxine. the method exhibited excellent specificity and acceptable accuracy. the average intra- and inter-assay coefficients of variation were 4.8 and 12.3%, resepectively. the fluorescence polarization immunoassay was compared with a radioimmunoassay (ria) using 96 clinica ...	1982	6754853
[cells in the epidermotropic lymphomas (mycosis fungoides and sézary syndrome. study using monoclonal antibodies].	the phenotypic expression of immunocompetent cells involved in lesions of cutaneous t-cell lymphoma (mycosis fungoides and sezary syndrome) was studied by using monoclonal antibodies specific for t-cell subsets and hla dr antigens (okt3, t4, t6, t8) (bl6, bl2). in sezary syndrome, the phenotype of sezary cell is homogeneous (t3+, t4+, t8-, t6-, bl2-) and corresponds to the phenotype expressed by the normal helper t-cell population. at ultrastructural level, the discontinuous distribution of okt4 ...	1982	6755391
high-frequency transduction of pbr322 by cytosine-substituted t4 bacteriophage: evidence for encapsulation and transfer of head-to-tail plasmid concatemers.		1982	6755510
endocrine circadian time structure in the aged.	thirteen circadian rhythms in plasma hormone levels (acth, aldosterone, cortisol, c-peptide, insulin, dhea-s, estradiol, lh, prolactin, 17-oh progesterone, testosterone, t4 and tsh) were studied in april 1981 in 25 males and 25 females 57 to 91 years of age, institutionalized at the berceni hospital for the aged. the radioimmunoassays and the statistical rhythmometric evaluation by the cosinor procedure were done at s. paul-ramsey medical center, st. paul, mn, usa. circadian rhythms were found a ...	1982	6755633
in vitro transcription of phage t4 late genes on purified dna by partially purified rna polymerase from t4-infected escherichia coli b cells.	rna polymerase was purified from 'late' phage t4-infected escherichia coli b cells by dna-cellulose affinity chromatography and high salt agarose filtration. the dna-cellulose-purified rna polymerase preparation contained t4-coded dna endonuclease activity and several proteins, some with sizes comparable with the known t4 maturation factors, essential for late rna synthesis. some of these proteins, and the dna endonuclease utilizing native, parental t4 dna and supercoiled phi x 174 dna as substr ...	1982	6756478
characteristics of a bacteriophage t4-induced complex synthesizing deoxyribonucleotides.	a preparation of bacteriophage t4-induced deoxyribonucleotide synthetase complex is described. this very large complex of enzymes can be separated by centrifugation at 100,000 x g, by sucrose step gradient centrifugation, or with molecular exclusion columns. by direct assay and by unidimensional and two-dimensional acrylamide electrophoretic separations the following t4-coded enzymes were shown to be associated with the complex: ribonucleoside diphosphate reductase, dcmp deaminase, dctp/dutpase, ...	1982	6757252
oligonucleotide-directed mutagenesis using m13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of dna.	this paper presents a versatile and efficient procedure for the construction of oligodeoxyribonucleotide directed site-specific mutations in dna fragments cloned into m13 derived vectors. as an example, production of a transition mutation in a clone of the yeast mata1 gene is described. the oligonucleotide is hybridized to the template dna and covalently closed closed double stranded molecules are generated by extension of the oligonucleotide primer with e. coli dna polymerase (large fragment) a ...	1982	6757864
escherichia coli dnag gene product is required for a normal rate of phage t4 dna synthesis.		1982	6758324
[functional activity of short fibrils of bacteriophage t4].		1982	6759084
identification of the origins of t4 dna replication.	two physical origins of t4 dna replication were determined by hybridization of viral dna prepared 2.5 min after infection to a display of total t4 dna. this is the earliest time after t4 infection of escherichia coli at 37 degrees c that labeled and hybridizable dna can be detected. the two origins, separated by about 25 kilobases, were identified and localized in the early region of the t4 map. one of them is located in a 5.6-kilobase ecori fragment containing genes 62-46. the other is located ...	1982	6760193
[characterization of a cacl2-dependent transfection system of escherichia coli and t3 phage dna].	transfection by dna isolated from bacteriophage t3 was studied using escherichia coli 921/0 as host. the following conditions were found optimal: competent e. coli 921/0 were obtained by harvesting the bacteria at the onset of late exponential growth (5 x 10(8) cells/ml) and treating the latter with 0.05 m cacl2. hereafter, the microbes were suspended in 50 mm tris-hcl buffer (ph 7.2) and the concentration adjusted to 7 x 10(9) cells/ml. t3 dna was added and the suspension kept at 0 degrees c fo ...	1982	6760569
[evaluation of enzymun-test t4 kit].		1982	6761015
[fundamental and clinical studies on the measurement of serum t4 and t4-uptake value by homogeneous enzyme inhibitor assay].		1982	6762267
torsional tension in intracellular bacteriophage t4 dna. evidence that a linear dna duplex can be supercoiled in vivo.		1982	6762444
homologous terminal sequences in the double-stranded rna genome segments of cytoplasmic polyhedrosis virus of the silkworm bombyx mori.	the 3'-terminal regions (20 to 32 residues) of the genome double-stranded rna (dsrna) segments of cytoplasmic polyhedrosis virus were sequenced. the dsrnas, which were labeled at their 3' termini by incubation with [5'-(32)p]pcp and t4 rna ligase, were denatured and resolved into the plus and minus strands by agarose-urea gel electrophoresis. ten single-stranded rnas thus obtained from the five dsrna segments iv, v, viii, ix, and x were sequenced by postlabeling methods. common 3'-terminal seque ...	1982	16789232
thyroid function tests under the effect of steroidal contraceptives compared to normal pregnancy.	the effects of the oral contraceptive pill on th blood and urinary levels of various hormones was studies using 5 groups of women. group 1 consisted of 59 women using combined contraceptive pills for 1 to 12 years under the commercial names of primovlar (25 cases) anovlar (19 cases) and ovral (15) cases; group 2 consisted of 50 pregnant women during the 1st trimester; group 3 consisted of 50 pregnant women during th 2nd trimester; group 4 consisted of 50 pregnant women during the 3rd trimester ...	1982	12266214
ultrastructural studies on purkinje cell maturation in the cerebellum of the frog tadpole during spontaneous and thyroxine-induced metamorphosis.	the maturation of purkinje cells in the cerebella of both thyroxine (t4)-induced and normally metamorphosing tadpoles was studied by transmission electron microscopy, with particular reference to the perikaryal changes. during the latter part of the prometamorphic phase, many purkinje cells showed hypertrophied apical cones filled with mitochondria, golgi elements and rosettes of ribosomes. in early metamorphic climax, the perikaryal cytoplasm displayed stratification, with an inner zone of peri ...	1982	12050840
t4 rna ligase as a nucleic acid synthesis and modification reagent.		1981	6765649
effect of thyroid status on beta-adrenoreceptors and muscarinic receptors in the rat lung.	1. the effects of thyroid status on the specific binding of the muscarinic ligand (-)-[3h]quinuclidinyl benzilate (qnb) and of the beta-adrenoreceptor ligand (-)-[3h]dihydroalprenolol (dha) in the adult rat lung were investigated. 2. the specific binding of (-)-[3h]quinuclidinyl benzilate (qnb) to lung membranes was saturable and the equilibrium dissociation constant (kd) determined from scatchard analysis was 54 pm. kinetic analysis of the binding of [3h]qnb yielded a kd of 42 pm. [3h]qnb bindi ...	1981	6279669
tna-directed deletion of the trp operon from rsf2124-trp in escherichia coli.	plasmid pmt-trp was constructed by digestion of rsf2124-trp with restriction endonuclease psti and ligation with t4 ligase. in pmt-trp about 78% of the dna of transposon tna from rsf2124-trp was deleted, and hence the gene for ampicillin resistance was lost. all trp- segregants from pmt-trp carriers in escherichia coli w3110 and its derivatives were found to have lost the entire plasmid. on the other hand, deletion plasmids which had lost the trp operon were found among trp- segregants from rsf2 ...	1981	6279768
kinetics and mechanism of the association of the bacteriophage t4 gene 32 (helix destabilizing) protein with single-stranded nucleic acids. evidence for protein translocation.		1981	6279865
incorporation into dna of the base analog 2-aminopurine by the epstein-barr virus-induced dna polymerase in vivo and in vitro.	the epstein-barr virus (ebv)-induced intracellular dna polymerase was assayed in vitro for the ability to utilize the mutagenic nucleotide analog 2-aminopurine deoxyribose triphosphate (d2aptp), incorporating it as the corresponding monophosphate into dna or poly[d)(a-t)] template. bacteriophage t4, lymphocyte alpha, and the ebv particle-associated dna polymerases were assayed simultaneously for direct comparison. unlike these three polymerases, which were capable of distinguishing between d2apt ...	1981	6278464
toxin t4(6) from ptychodiscus brevis (formerly gymnodinium breve) enhances activation of voltage-sensitive sodium channels by veratridine.		1981	6262621
dna sequence of the transfer rna region of bacteriophage t4: implications for transfer rna synthesis.	sequences encoding eight trnas and two stable rnas of bacteriophage t4 are grouped together on the t4 genome in two clusters, separated by approximately 500 base pairs. the dna sequence of part of this region was determined. within each cluster coding sequences are separated by only one or a few base pairs. these findings imply that the rnas may be processed from a single multimeric transcript, with initial endonucleolytic cleavages generating the previously characterized monomeric and dimeric p ...	1981	6262781
cloned genes for bacteriophage t4 late functions are expressed in escherichia coli.		1981	6276568
physical mapping of bacteriophage t4.		1981	6276698
studies of the mechanism of bacteriophage t4 dna encapsidation.		1981	6276887
interactions between the maturation protein gp17 and the single-stranded dna binding protein gp32 initiate dna packaging and compete with initiation of secondary dna replication forks in phage t4.	since t4 dna, which is being packaged is actively replicating and recombining, we have asked whether replication and recombination proteins interact with maturation proteins. we report here on genetic evidence for interaction of the single-stranded dna binding protein gp32 with the maturation protein gp17. based on these and other results we suggest a model in which dna packaging is initiated from recombinational intermediates.u	1981	6276888
studies on the function of gene 49 controlled endonuclease of phage t4 (endonuclease vii).		1981	6276889
incidence of hypothyroidism after 131i therapy for hyperthyroidism. relation to pretherapy serum levels of t3, t4 and thyroid antibodies.	a correlation is reported between serum levels of triiodothyronine (s-t3) and thyroxine (s-t4) before treatment, as well as levels of thyroid antibodies before treatment, and the development of hypothyroidism following 131i therapy in 86 patients with hyperthyroidism. patients with marked elevation of s-t3 or s-t4 had demonstrable antibodies to thyroid cytoplasmic antigen more often than those with normal or moderately elevated levels, and patients with markedly elevated levels of s-t3 also had ...	1981	6277151
advanced carcinoma of the nasopharynx. treatment results.	from 1947 through 1977, 93 patients with carcinoma of the nasopharynx were treated. most (83%) of the patients, had stage iv disease including 9 per cent who had a systemic tumor dissemination. cervical lymph node metastases were present in 73 (78%) patients. the 5-year actuarial survival for all 93 patients was 13 per cent, 40 for stage ii, 18 for stage iii and 10 for stage iv patients. the factors with significant influence on survival were initial performance status and t4 disease. the presen ...	1981	6277153
the effect of burst patterning of preganglionic input on the efficacy of transmission at the cat stellate ganglion.	1. the effect of burst patterned preganglionic stimulation on the efficacy of transmission at the cat stellate ganglion in vivo was studied to determine whether the increase in acetylcholine (ach) release that occurs at preganglionic terminals with similar stimulation is synaptically active.2. stimulation of the whole or a portion of the thoracic sympathetic trunk between rami t4 and t5 with recording of evoked compound action potentials in the inferior cardiac nerve yielded a preparation with a ...	1981	6275070
hypothalamic control of endocrine thermogenesis.	hypothalamic and ruminal cooling raised serum thyrotropin (tsh), adrenocorticotropin (acth), norepinephrine (ne), and glucose in conscious goats in 20 degree c ambient temperature. cooling of the preoptic anterior hypothalamus (poah) for 2 h initially evoked shivering and vasoconstriction, leading to 1.5 degree c rise in rectal temperature (tr). pituitary-thyroid activation by poah cooling was shown by peak rises in tsh of 60% at 40 min, in triiodothyronine (t3) of 54% at 80 min, and in thyroxin ...	1981	6275712
beta 2 receptor-mediated stimulation of friend erythroleukemia cell growth by thyroid hormones.	thyroid hormones are known to enhance normal erythroid colony growth (cfue) and this enhancement depends on a functional beta 2-adrenergic receptor mechanism. we investigated the response of friend cells to thyroid hormones, catecholamines, and other compounds influencing cellular camp activity. the thyroid hormones l-t3, l-t4, and "reverse t3" stimulated erythroleukemia colony growth in a serum-substituted methylcellulose culture system with peak activity at 10(-7) m. various beta-adrenergic co ...	1981	6276212
[the effect of thyroid hormones on low density lipoprotein receptors in cultured human skin fibroblasts (author's transl)].	the catabolic rate of low density lipoprotein (ldl) has been shown to be delayed in hypothyroidism and increased in hyperthyroidism. ldl catabolism is mainly mediated through the ldl receptor pathway in the parenchymal cells. in the present paper the effect of thyroid hormones in ldl receptors was studied in cultured human skin fibroblasts. human fibroblasts were established from 2 normal subjects, one heterozygous patient and 2 homozygous patients with familial hypercholesterolemia (fh). human ...	1981	6276236
partial exclusion of bacteriophage t2 by bacteriophage t4: induction of early enzymes by excluded t2.	in crosses between bacteriophages t2 and t4 most early genes of t2 are partially excluded from the progeny. six genes of t4 affect the exclusion of six corresponding exclusion-sensitive sites in t2, each gene being specific for the exclusion of one site. mutants of t4 in these genes have been isolated (ex mutants). the induction of the gene product (deoxycytidine triphosphate nucleotidohydrolase, dctpase) of the strongly excluded t2 gene 56 was determined. the dctpase was induced in the presence ...	1981	6273500
rii cistrons of bacteriophage t4. dna sequence around the intercistronic divide and positions of genetic landmarks.		1981	6273585
selective inhibition by harmane of the apurinic apyrimidinic endonuclease activity of phage t4-induced uv endonuclease.	1-methyl-9h-pyrido-[3,4-b]indole (harmane) inhibits the apurinic/apyrimidinic (ap) endonuclease activity of the uv endonuclease induced by phage t4, whereas it stimulates the pyrimidine dimer-dna glycosylase activity of that enzyme. e. coli endonuclease iv, e. coli endonuclease vi (the ap endonuclease activity associated with e. coli exonuclease iii), and e. coli uracil-dna glycosylase were not inhibited by harmane. human fibroblast ap endonucleases i and ii also were only slightly inhibited. th ...	1981	6273822
purified glucocorticoid receptors bind selectively in vitro to a cloned dna fragment whose transcription is regulated by glucocorticoids in vivo.	activated glucocorticoid receptor protein, purified to 40-60% homogeneity from rat liver extracts, binds selectively in vitro to a cloned fragment of murine mammary tumor virus (mtv) dna. the dna fragment tested contains about half of the sequences present in intact mtv dna, and its rate of transcription, like that of the intact viral element, is strongly stimulated by glucocorticoids when it is introduced into the genome of a receptor-containing cell. in contrast, the receptor fails to bind sel ...	1981	6273877
some properties of the human erythrocyte receptors for neisseria gonorrhoeae.	the nature of the receptors for t1 and t4 neisseria gonorrhoeae on erythrocytes and other cells was investigated. in general, cells of nonprimate origin contained few receptors for gonococci. receptors for t4 gonococci were only uncovered when host cells were pretreated with trypsin. trypsinization, while unnecessary for t1 adherence to erythrocytes, enhanced attachment in inverse proportion to original erythrocyte sensitivity. receptors for t1 and t4 organisms on trypsinized and trypsin-neurami ...	1981	6274494
primary structure of the low molecular weight nucleic acid-binding proteins of murine leukemia viruses.	murine leukemia viruses contain a low molecular weight basic protein, designated p10, which binds to single-stranded nucleic acids. the complete amino acid sequence of p10 from the rauscher strain of virus has been determined. the partial amino acid sequences of p10s from moloney, friend, akr, gross, radiation leukemia, and balb/2 viral strains have also been determined using microsequencing techniques. rauscher p10 is composed of 56 amino acid residues; the other p10s are similar in size but di ...	1981	6267042
t4 phage-coded dihydrofolate reductase. subunit composition and cloning of its structural gene.	the structures of phage-coded dihydrofolate reductases are of interest because of 1) possible relationship to plasmid-coded dihydrofolate reductases; 2) unusual regulation of enzyme synthesis; and 3) multiple roles and intermolecular interactions involving the protein. to prepare for primary structural studies, we have cloned the t4 frd gene, which codes for dihydrofolate reductase, and we have determined for redetermined some physical properties of the enzyme. the native enzyme has a molecular ...	1981	6267063
a homogeneous type ii dna topoisomerase from hela cell nuclei.	using kinetoplast dna networks as a substrate in a decatenation assay, we have purified to apparent homogeneity a type ii dna topoisomerase from hela cell nuclei. the most pure preparations contain a single polypeptide of 172,000 daltons as determined by sodium dodecyl sulfate-gel electrophoresis. the molecular weight of the native protein, based on sedimentation and gel filtration analyses, is estimated to be 309,000. these results suggest that the enzyme is a dimer of 172,0090-dalton subunits. ...	1981	6267071
studies on the biochemical basis of mutation vi. selection and characterization of a new bacteriophage t4 mutator dna polymerase.		1981	6267293
sensitive determination of pyrimidine dimers in dna of uv-irradiated mammalian cells. introduction of t4 endonuclease v into frozen and thawed cells.	endonuclease v from e. coli infected with phage t4 was used to evaluate the frequency and the removal of pyrimidine dimers from dna in cultured mammalian cells. cellular membranes were made permeable to the enzyme by two cycles of rapid freezing and thawing. the number of endonuclease-sensitive sites in dna was assayed by sedimentation in alkaline sucrose gradients upon which the cells were lysed directly. comparison of the frequency of endonuclease-sensitive sites with the frequency of pyrimidi ...	1981	6267456
[peroxidase and human thyroid hormone synthesis disorders (author's transl)].	thyroid peroxidase in involved in several steps of the biosynthesis of thyroid hormone utilizing h2o2: peroxidation of iodide to iodine, iodination of thyroglobulin (tg) and coupling reaction leading to t4 and t3 formation. the peroxidase enzyme appears to be an heme protein containing a protoporphyrin ix, with binding sites for both iodide and tyrosine. although the peroxidase is present in numerous cellular structure, iodination activity occurs primarily if not only at all, at the apical cell ...	1981	6267713
inhibitor studies of phage t4 wild-type and mutant dna polymerases. v. a. summary of kinetic and inhibitor data.	the dna polymerases of phage t4 wild-type, the mutator mutant l98 and the antimutator mutant cb121 were purified about 100-fold free of foreign enzyme activities interfering with the polymerase assay. the enzymes were characterized as to thermostability, exonuclease activity and kinetic data with dna template primer, deoxythymidine 5' -triphosphate, and a mixture of all four deoxynucleoside 5' -triphosphates. the effects of eight inhibitors of dna synthesis on the three enzymes were determined ( ...	1981	6267833
the role of t3 and its receptor in efficient metabolisers receiving very-low-calorie diets.	obese patients taking a 1.34 mj/day formula diet showed a steady decline in the rate of weight loss after several weeks and this was associated with a fall in rmr, serum t3 levels and the number of t3 receptors on peripheral lymphocytes. the addition of t3 (60 micrograms/day) to the dietary regime produced a significantly greater weight loss by the 12th week. analysis of individual thyroid hormone levels revealed those patients taking the t3 tablets who achieved the greatest weight loss had the ...	1981	6268557
thyroid hormone binding to plasma membrane preparations: studies in different thyroid states and tissues.	two orders of high-affinity saturable binding sites for l-t4 and l-t3 were evidenced in purified plasma membrane preparations from rat liver (apparent equilibrium dissociation constant kd for t4 congruent to 0.6 and 23 nm, for t3 congruent to 9 and 237 nm) and kidney (kd for t4 congruent to 4 and 127 nm; for t3 congruent to 15 and 270 nm). differences of statistical significance were only found for the higher affinity t4 binding site. in contrast, no saturable t4 or t3 binding could be detected ...	1981	6268692
investigations on myelination in vitro: regulation of 2,3'-cyclic nucleotide 3'-phosphohydrolase by thyroid hormone in cultures of dissociated brain cells from embryonic mice.	the direct influence of l-3,3',5-triiodothyronine (t3) on the development of 2',3'-cyclic nucleotide 3'-phosphohydrolase (ec 3.1.4.37, cnpase) is demonstrated by using an in vitro culture system of dissociated embryonic mouse brain cells. serum from a thyroidectomized calf, which contained low levels of t3 (31 ng/100 ml), and thyroxine, t4 (less than 1 micrograms/ml), was used in the culture medium in place of normal calf serum (t3, 103 ng/100 ml; t4, 5.7 micrograms/ml) to render the culture res ...	1981	6268753
regulation of expression of cloned bacteriophage t4 late gene 23.	the parameters governing the activity of the cloned t4 gene 23, which codes for the major t4 head protein, were analyzed. suppressor-negative bacteria carrying wild-type t4 gene 23 cloned into plasmid pcr1 or pbr322 were infected with t4 gene 23 amber phage also carrying mutations in the following genes: (i) dena and denb (to prevent breakdown of plasmid dna after infection) and (ii) dena, denb, and, in addition, 56 (to generate newly replicated dna containing dcmp) and alc/unf (because mutation ...	1981	6268820
[restriction mapping of t4 bacteriophage late gene region which contains the origins of dna replication].	dnas of lambda t4 recombinants 596-27 (genes 50-5), 596-30 (genes 50-8), 596-29 (genes 50-12), 591-16 (genes 6-8), 591-1 (genes 9-12), 596-13 (genes 13-16), 596-17 (genes 18-20) and 596-11 (genes 25-29) were mapped with the use of ecori, hindiii, smai, sali and bamhi restriction enzymes. t4 dcdna was digested with hindiii restriction endonuclease and resulting fragments were cloned into hindiii lambda vector 761. the recombinants 761-7, 761-17, 761-19, 761-24, 761-44, 761-50, 761-55 contained th ...	1981	6268966
specific and reversible inhibition of the blunt end joining activity of the t4 dna ligase.	specific, complete and reversible inhibition of the joining of blunt ended dna duplexes catalyzed by the t4 dna ligase can be obtained by using atp, the enzyme cofactor, at concentrations of 5 mm and higher. on cohesive dna ends, 5 mm atp, which completely inhibits blunt end ligation, brings about only a limited (8%) reduction in the level of joining obtainable under optimal atp concentration (0,5 mm or lower). a similar but less drastic uncoupling of the two kinds of joining can be achieved at ...	1981	6269089
effect of thiol blocking agents on the binding of t3 and t4 in rat liver nuclear extract.	liver nuclei bind thyroid hormones (t3 and t4) with different binding affinities. in out studies it was estimated that the apparent association constant (ka) in the liver nuclei for t3 was 8.8 x 10(10) l mol-1 and for t4 was 4.1 x 10(9) l mol-1. we have found that the binding sites for t4 are also saturated by an excess of unlabelled t4 and that saturation was dependent on the purification step of liver binding proteins. in liver nuclear extracts with 0.4 mol l-1 kcl the specific binding of t3 a ...	1981	6269345
diabetes, hypophysectomy, or thyroidectomy reduces nuclear l-triiodothyronine-binding capacity of rat lung.	l-t3 (t3) receptors of the lungs of rats were found to be under hormonal control. the apparent binding capacity of t3 receptors of lungs of diabetic, hypophysectomized, and thyroidectomized rats were 0.224 +/- 0.009, 0.195 +/- 0.007, and 0.102 +/- 0.008 pmol/mg dna, as compared to 0.0321 +/- 0.014 pmol/mg dna for normal animals. the binding affinities for t3 in normal, diabetic, hypophysectomized, and thyroidectomized rats were 256 +/- 3.24, 270 +/- 3.81, 249 +/- 4.03, and 260 +/- 8.2 pm, respec ...	1981	6263588
the stereochemical course of thiophosphoryl group transfer catalyzed by t4 polynucleotide kinase.	the stereochemical course of the phosphoryl transfer reaction catalyzed by t4 polynucleotide kinase has been determined using the chiral atp analog, (sp)-adenosine-5'-(3-thio-3-[18o]triphosphate). t4 polynucleotide kinase catalyzes the transfer of the gamma-thiophosphoryl group of (sp)-adenosine-5'-(3-thio-3-[18o]triphosphate) to the 5'-hydroxyl group of apa to give the thiophosphorylated dinucleotide adenyl-5'-[18o]phosphorothioate-(3'-5')adenosine. a sample of adenyl-5'-[18o]phosphorothioate-( ...	1981	6263897
the synthesis and cloning of two tyrosine suppressor trna genes with altered promoter sequences.	the total synthesis of the suppressor trna gene (ssu1) containing a 51-base pair long "natural" promoter has previously been reported. in this paper, we describe the synthesis and characterization of two genes (ssu3 and ssu4) in which the promoter sequence present in the parent ssu1 gene has been altered. the modifications, introduced as a part of the study of structure-function relationships, were placed in the two known prominent regions of homology. the change introduced in ssu3 was a g:c to ...	1981	6263904
early steps of excision repair of cyclobutane pyrimidine dimers by the micrococcus luteus endonuclease. a three-step incision model.	the early steps of excision repair of cyclobutane pyrimidine dimers are investigated. it is demonstrated that the apurinic/apyrimidinic endonuclease associated with the micrococcus luteus uv-specific endonuclease cleaves the phosphodiester bond on the 3' side of the deoxyribose leaving a 3' hydroxy terminus and a 5' phosphoryl terminus. this nick is not a substrate for t4 polynucleotide ligase. the 3' base-free deoxyribose terminus is not a substrate for either the polymerase or the 3' to 5' exo ...	1981	6263931
identification and characterization of nuclear 3,5,3'-triiodothyronine-binding sites in fetal human lung.	thyroid hormones have been implicated in the prenatal lung development of several species. to investigate the possibility that thyroid hormones could play a role in human lung development, we examined nuclei from fetal human lung for the presence of high affinity t3-binding sites. a single class of high affinity (kd = 35 +/- 3 pm) binding sites for l-t3 was identified in nuclei from fetuses between 12-19 weeks of gestation. the t3-binding capacity increased 65% between 12-13 weeks (binding capac ...	1981	6263937
map of restriction sites on bacteriophage t4 cytosine-containing dna for endonucleases bamhi, bglii, kpni, pvui, sali, and xbai.	a complete map of the cleavage sites of restriction endonucleases bamhi, bglii, kpni, pvui, sali, and xbai was determined for the cytosine-containing dna of a bacteriophage t4 alc mutant. the 56 sequence-specific sites were assigned map coordinates based on a least-squares analysis of measured fragment lengths. altogether, the lengths of 118 fragments from single and double enzyme digestions were measured by electrophoresis of the fragments in agarose gels. dna fragments of known sequence or dna ...	1981	6264096
alignment of a restriction map with the genetic map of bacteriophage t4.	a restriction map of the bacteriophage t4 genome was aligned with the t4 genetic map. included were the cleavage sites for bamhi, bglii, kpni, pvui, sali, and xbai. the alignment utilized the fact that the t4 genetic map had been oriented previously with respect to a t2/t4 heteroduplex map. dna fragments from a bglii digestion of cytosine-containing dna from a t4 dctpase- dena denb(riih23b) alc mutant were hybridized with full-length chromosomal strands of bacteriophage t2, and the heteroduplexe ...	1981	6264097
[partial resistance to thyroid hormones in a patient with a euthyroid goiter (author's transl)].	this case report deals with a 40 year-old female with goiter, elevated levels of peripheral thyroid hormones without the clinical picture of hyperthyroidism, and an exaggerated response to tsh and trh. the fact that a feedback conversion appeared after progressive doses of t3 is outlined. taking into account the elevated t3/t4 ratio, the high rt3, and the fact that all other pituitary hormones remained normal in the presence of increased levels of thyroid hormones, it is concluded that the case ...	1981	6264238
alterations in t3 and t4 receptor binding in fasting and diabetes mellitus.		1981	6264258
[plasma filtration treatment in thyreotoxic crisis].	a 65-year-old man was treated three times in 4 consecutive days with plasma filtration for a severe thyroid storm. during the treatment the serum and filtrate levels of thyroxine, triiodothyronine, free t4, free t3, tbg, albumin and reverse-t3 were measured, and the results showed that, apart from reduction of the total hormone amount, the supply of binding sites for the free hormones is an important aspect in the treatment of thyroid storm.	1981	6261323
[clinical trial of sodium levothyroxine in the treatment of hypothyroidism in adults (author's transl)].	the authors present the results of a clinical trial performed on 44 patients with hypothyroidism. all were treated with l-thyroxine (l-t4) during 6 months. in 39 patients out of the 44 in this trial, clinical and hormonal balance was achieved. tolerance was excellent and no clinical evidence of overdosage was observed, comprising the 11 patients whose plasma t4 values were slightly exceeding normal values. the optimal substitution dosage most commonly applied, was on the average of 150 microgram ...	1981	6261336
studies on t4-head maturation. 1. purification and characterization of gene-49-controlled endonuclease.	an endonuclease has been purified more than 300-fold from escherichia coli infected with bacteriophage t4. the enzyme degrades rapidly sedimenting (greater than 1000 s) dna in vitro by introducing a limited number of breaks. the substrate is the replicative dna isolated from cells infected with gene-49-defective phage [kemper, b, and janz, e. (1976) j. virol. 18, 992-999]. molecules of approximately a third the size of unit-length t4 dna are exclusively found in a limit digest. the enzyme also r ...	1981	6262077
studies on t4-head maturation. 2. substrate specificity of gene-49-controlled endonuclease.	the substrate specificity of 49+-enzyme was investigated in vitro. the enzyme showed a marked preference for rapidly sedimenting t4 dna (greater than 1000 s) when helix-destabilizing proteins from escherichia coli or phage t4 were added to the reaction. regular replicative t4 dna (200-s dna) or denatured t4 dna was not cleaved by the enzyme in the presence of these proteins but if they were omitted from the reaction both dnas become good substrates for the enzyme. 200-s dna was cleaved at its na ...	1981	6262078
physical association of pyrimidine dimer dna glycosylase and apurinic/apyrimidinic dna endonuclease essential for repair of ultraviolet-damaged dna.	t4 endonuclease v [endodeoxyribonuclease (pyrimidine dimer), ec 3.1.25.1)], which is involved in repair of uv-damaged dna, has been purified to apparent physical homogeneity. incubation of uv-irradiated poly(da).poly(dt) with the purified enzyme preparations resulted in production of alkali-labile apyrimidinic sites, followed by formation of nicks in the polymer. the activity to produce alkali-labile sites was optimal in a relatively broad ph range (ph 6.0-8.5), whereas the activity to form nick ...	1981	6265906
expression of the denv gene of bacteriophage t4 cloned in escherichia coli.	the denv gene of bacteriophage t4 has been cloned into escherichia coli k-12 by inserting appropriate fragments of cytosine-containing t4 dna into the sal i site of the plasmid pbr322. the denv gene codes for an enzyme that initiates the excision repair of pyrimidine dimers produced in dna by uv. in uvra reca mutants, deficient in an early step in excision repair, the cloned dna results in enhanced uv resistance that is more pronounced in stationary- than in exponential-phase cultures. the expre ...	1981	6265912
[effects of chronic administration of lithium on thyroxine (t4), triiodothyronine (t3), reverse t3 (rt3) free thyroxine ratio (ftr) and thyreostimuline (tsh) in thyrotoxicosis (author's transl)].	fourteen hyperthyroid subjects were studied between 3 and 141 days. the reduction of thyrotoxicosis is rarely obtained (2 cases), mainly because on the one hand, correct plasma lithium level (0.7-1.2 meq/l) is rarely obtained (3 cases only), on the other hand because a high dose of lithium is required, perhaps owing to high clearance of lithium in thyrotoxicosis. the indications of lithium use in thyrotoxicosis are reviewed.	1981	6266038
the control of peroxidase-catalysed iodination and de-iodination.	it has been demonstrated that the h2o2/l ratio is a critical factor in the control of iodination and de-iodination of covalently bound tyrosyl residues in proteins and free iodotyrosines by peroxidase enzymes. this has been shown for myeloperoxidase (mpo) isolated from normal human polymorphonuclear lymphocytes in particular, and also for peroxidase of animal origin such as thyroid peroxidase (tpo) and lactoperoxidase (lpo). it has been shown that the h2o2/l ratio exerts a controlling influence ...	1981	6266056
binding of netropsin to dna in complexes with polypeptides containing repetitive lysine sequences.	the interaction of the antibiotic netropsin with calf thymus dna, t4 dna and poly(da-dt) . poly(da-dt) in complexes with sequential polypeptides containing repetitive lysine sequences and histone h1 was investigated using circular dichroism spectroscopy and equilibrium dialysis. both soluble dna-polypeptide complexes and insoluble complexes showed binding of netropsin. the possibility of displacement of polypeptides from dna binding sites by competition with netropsin molecules was eliminated by ...	1981	6269619
dissociation of serum triiodothyronine concentration and hepatic nuclear triiodothyronine-binding capacity in streptozotocin-induced diabetic rats.	to determine the relationship between the concentrations of serum t3 and hepatic nuclear t3 receptor in a reversible nonthyroidal disease, we studied these parameters in rats with streptozotocin-induced diabetes mellitus, control rats, and rats that were weight-matched to the diabetic rats by food restriction. results were similar either 1 or 9 weeks after the induction of diabetes. a significant decrease from control in mean serum t4, t3 and free t3 index was noted in the diabetic rats. however ...	1981	6269840
epinephrine deficiency in hypocorticotropic hypopituitary children.	in rats, adrenal medullary synthesis of epinephrine is impaired by acth deficiency and is not improved by replacement doses of glucocorticoid. we have evaluated plasma epinephrine and norepinephrine concentrations in 43 children, 8-15 yr old. these children were divided into 5 groups, with 6-10 per group: normals; children with isolated gh deficiency; hypopituitary children deficient in both gh and tsh; hypopituitary children deficient in gh, tsh, and acth; and short children without known organ ...	1981	6270172
evidence that the uv endonuclease activity induced by bacteriophage t4 contains both pyrimidine dimer-dna glycosylase and apyrimidinic/apurinic endonuclease activities in the enzyme molecule.	we performed experiments to determine whether the phage t4-induced uv endonuclease activity is a single protein containing both pyrimidine dimer-dna glycosylase and apyrimidinic endonuclease activities. the uv endonuclease activity is induced by the denv gene and codes for the glycosylase activity. we obtained several kinds of evidence that the protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity. after chromatography on deae-cellulose, the two activit ...	1981	6270374
den v gene of bacteriophage t4 codes for both pyrimidine dimer-dna glycosylase and apyrimidinic endonuclease activities.	recent studies have shown purified preparations of phage t4 uv dna-incising activity (t4 uv endonuclease or endonuclease v of phage t4) contain a pyrimidine dimer-dna glycosylase activity that catalyzes hydrolysis of the 5' glycosyl bond of dimerized pyrimidines in uv-irradiated dna. such enzyme preparations have also been shown to catalyze the hydrolysis of phosphodiester bonds in uv-irradiated dna at a neutral ph, presumably reflecting the action of an apurinic/apyrimidinic endonuclease at the ...	1981	6270375
deficiency of t helper cells in transient hypogammaglobulinemia of infancy.	we studied 17 patients with transient hypogammaglobulinemia of infancy to define the immunologic defect responsible for this disorder. the number of circulating b cells in these patients was normal, as was the ability of the b cells to synthesize immunoglobulins when stimulated with epstein-barr virus, a direct b-cell activator. however, the capacity of the b cells to synthesize igg in response to pokeweed mitogen, a t-cell-dependent b-cell activator, was depressed. experiments with cultured lym ...	1981	6270560
sequences of the ssb gene and protein.	we have determined the sequences of the ssb gene and protein of escherichia coli. the coding region of ssb is 534 base pairs and is preceeded and followed by dyad symmetries of 39 base pairs and 27 base pairs, respectively. the promoter for ssb is close to that for uvra and these two genes are transcribed in opposite directions: ssb clockwise and uvra counterclockwise on the standard e. coli genetic map. the dna helix-destabilizing protein encoded by the ssb gene (single-strand binding protein) ...	1981	6270666
[treatment of primary hypothyroidism in adults : dosage schedule and biological surveillance (author's transl)].	hypothyroidism in a disease with a poor prognosis, especially as the disorder is often unrecognised, or is treated empirically and without adequate supervision. modern biological tests can establish the diagnosis, even in subclinical cases, and can also ensure close supervision of dosage to avoid long-term adverse effects of too low or too high dosage regimens. the authors prefer dl thyroxine (dl t4) to other currently employed products : wide variations in triiodothyronine (t3) activity in thyr ...	1981	6270812
characterization of bacteriophage t4 and d rna, a low-molecular-weight rna of unknown function.		1981	6271070
[biosynthesis of early enzymes induced by bacteriophage t4].	the biosynthesis of early enzymes of dna-ligase, rna-ligase, dna-polymerase, polynucleotide kinase, exonuclease a induced by bacteriophage t4amn82 was studied. the maximal activity of dna-ligase was observed at the 60th min after the infection, while that of the other enzymes was revealed at the 90th min and reached 4, 45, 529, 120 and 78 units per mg of protein for dna-ligase, rna-ligase, polynucleotide kinase, dna-polymerase and exonuclease a, respectively. bacteriophage t4amn82 induced the ma ...	1981	6271263
decreased thyroidal response to thyrotropin in diabetic mice.	the effect of diabetes mellitus on the synthesis and secretion of thyroid hormone ws investigated in mice with streptozotocin-induced diabetes. thyroid glands were labeled in vivo with 131i for 2 h. in control animals, tsh stimulated the synthesis of pb127i and 131i-labeled iodothyronines and simultaneously decreased the proportion of 131i-. these effects of tsh were not observed in diabetic animals but were demonstrable in diabetic animals treated with insulin. for studies of hormone secretion, ...	1981	6271530
the ontogeny of the thyrotropin-thyroid axis in early bovine embryos.	t4 and t3 formation and their response to tsh, camp, and prostaglandin e2 (pge2) stimulation were studied by ria in cultured bovine fetal thyroids from 130 embryos of 1.2-25.0 cm crown-rump length (crl). t4 and t3 were found in all of the freshly isolated glands studied, and their concentrations increased significantly (p less than 0.05) during a 24-h incubation of glands from fetuses with a crl greater than 8.0 cm. the release of t4 (nanograms per mg tissue), but not of t3, increased consistent ...	1981	6271537
goitrous hypothyroidism due to iodide-trapping defect.	a 32-yr-old man with goitrous hypothyroidism due to an iodide-trapping defect is described. he was admitted because of goiter which had been increasing in size. his parents were unrelated, and no cases of goiter were found in his family. on admission, serum t3 was 39 ng/dl, serum t4 was 1.0 micro g/dl, and serum tsh was 217 micro u/ml. his 24-h thyroidal 131 i uptake was 0.05%. antithyroid antibodies were negative. in a tracer study, the thyroidal 131 i uptakes were 6.3% at 2 h, 4.0% at 6 h, and ...	1981	6271803
conversion and binding of tetraiodothyronine in developing rat brain.	in this study we have examined whether rat brain nuclear thyroid hormone receptors bind t4 or metabolites of t4 and whether there is a developmental change in brain t4 metabolism and binding. developing animals were injected with trace [125i]3',5'-tetraiodothyronine ([125i]t4) and after sacrifice brain nuclear and cytoplasmic fractions were examined to determine whether their radioactivity was represented by the infected [125i]t4 or any of its metabolites. of the radiothyronines specifically bou ...	1981	6272146
anthramycin inhibition of restriction endonuclease cleavage and its use as a reversible blocking agent in dna constructions.	anthramycin can form a stable complex with dna which does not dissociate upon repeated ethanol precipitations. the complex forms in less than one hour at ph 5.5. bound anthramycin seems to be located in the minor groove of the dna helix in the anthramycin dna complex, since methylation of adenosine residues at n-3 by dimethylsulfate is reduced. the anthramycin-dna complex is resistant to digestion by an excess of a number of restriction enzymes. anthramycin can be removed from dna by incubation ...	1981	6272225
dna flexibility studied by covalent closure of short fragments into circles.	the ring closure probability, or j factor, has been measured for dna restriction fragments of defined sequence bearing ecori cohesive ends and ranging in size from 126 to 4361 base pairs (bp). the j factor is defined as the ratio of the equilibrium constants for cyclization and for bimolecular association via the cohesive ends. the end-joining reactions are fast compared to covalent closure of the cohesive ends by t4 dna ligase. the rate of ligase closure is shown to be proportional to the equil ...	1981	6272277
presence of gangliosides in the structure of membrane receptor for thyrotrophin.	thyroid gangliosides were isolated from bovine thyroid tissue. neutral lipids and glycolipids were removed by chromatography on a deae-cellulose column and residual acidic phospholipids by alkaline degradation. thyroid gangliosides were separated by chromatography on a silica acid column into 9 different fractions. thyroid gangliosides were found to be active in the inhibition of [125i]tsh binding to thyroid plasma membranes and this results from a direct action between thyrotrophin and ganglios ...	1981	6272516
[clinical and laboratory features of endemic goiter in the man region (ivory coast) (author's transl)].	an epidemiological study involving 616 subjects carried out in the man region, côte d'ivoire, showed endemic goiter in 54,5% of the group overall and in 80% of females. thyroid function of subjects chosen at random in the goitrous and non-goitrous group (105 g and 71 ng respectively) was identical. compared with a french control group t4 was significantly lower but t3 and tsh were higher. tbg level is high with low iodine elimination. high levels of tsh are not necessarily associated with low t3 ...	1981	6285798
the stereochemical course of phosphoryl transfer catalysed by polynucleotide kinase (bacteriophage-t4-infected escherichia coli b).	polynucleotide kinase (bacteriophage-t4-infected escherichia coli b) catalyses the transfer of the [gamma-16o,17o,18o]phosphoryl group from 5'[gamma(s)-16o,17o,18o]atp to 3'-amp with inversion of configuration at the phosphorus atom. the simplest interpretation of this observation is that the [gamma-16o,17o,18o]phosphoryl group is transferred directly from atp to the co-substrate by an 'in-line' mechanism.	1981	6279097
investigations on myelination in vitro. regulation of sulfolipid synthesis by thyroid hormone in cultures of dissociated brain cells from embryonic mice.	l-3,5,3'-triiodothyronine (t3) has been shown to influence the synthesis of myelin-associated lipids in cultures of cells dissociated from brains of embryonic mice (bhat, n. r., sarlieve, l., subba rao, g., and pieringer, r. a. (1979) j. biol. chem. 254, 9342-9344). this culture system was used in the present study to gain additional information on the regulation of the synthesis of myelin lipids by thyroid hormone. the rate of synthesis of the myelin associated sulfolipids remained drastically ...	1981	6256388
primary structure of the bacteriophage t4 dna helix-destabilizing protein.	the amino acid sequence of the single-stranded dna-binding protein encoded by gene 32 of bacteriophage t4 has been determined by manual and automated sequencing of peptides derived from cyanogen bromide cleavage and digestion with trypsin, chymotrypsin, and staphylococcal protease. tryptic digestion of citraconylated or succinylated gene 32 protein yields five peptides containing 4, 27, 42, 65, and 163 residues, respectively, which can be separated by sephadex chromatography. each of these trypt ...	1981	6257686
porcine granulosa cells in suspension culture. i. follicle-stimulating hormone induction of human chorionic gonadotropin-binding sites on cells from small follicles.	granulosa cells were aspirated from small follicles from the ovaries of 3- to 6-month-old pigs, washed, and cultured in sealed spinner flasks. medium 199 was supplemented with 10% fetal bovine serum, insulin, dexamethasone, t4, and antibiotics. fsh, lh, or hcg was added daily where appropriate. cultures were sampled every other day for 10 days. cells were examined microscopically, and cellular [125i]hcg binding and dna content were measured. the cells aggregated and underwent morphological lutei ...	1981	6258909
some substrate properties of analogues of oligothymidylates with p-s-c5' bonds.	the action of t4 polynucleotide kinase, t4 dna polymerase, e. coli dna polymerase i, snake venom phosphodiesterase (vpde) and s1 nuclease on analogues of oligothymidilates with p-s-c5' bonds and the ability of these analogues to prime the replication of poly (da) by t4 dna polymerase were studied. these analogues were shown to be substrates for all these enzymes. substitution of these analogues for corresponding oligothymidilates in the reaction mixtures resulted in drop in rates of enzymic reac ...	1981	6259619