Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| t cell subsets in allograft rejection. in situ characterization of t cell subsets in human skin allografts by the use of monoclonal antibodies. | we have provided evidence that both major t cell subsets, t4-positive (helper/inducer) and t8-positive (cytotoxic/suppressor), infiltrate human skin allografts. overall, and in the graft dermis and graft bed, t4-positive cells were predominant (1.5 to 3 times more numerous than t8-positive cells). in contrast, t8-positive cells were relatively more numerous in the epidermis and hair follicles. rejection probably proceeded by two apparently independent pathways: 1) direct contact killing of graft ... | 1982 | 6213716 |
| [t-lymphocyte subpopulations in chronic inflammatory rheumatism]. | monoclonal antibodies to a series of human lymphocytes subpopulations antigens (okt) specific of the peripheral t cells (okt3), of the inducer population (okt4) of the suppressor cytotoxic population (okt 5, okt8), and anti-ia (oki1) were used to study blood lymphocytes from 50 patients with active or inactive rheumatoid arthritis (ra) and from 23 patients with ankylosing spondylitis (as). in ra patients, there is a significant decrease of the total t cell population (t3 +) with higher percentag ... | 1982 | 6213999 |
| in vitro replication of mutagen-damaged dna: sites of termination. | we have examined the effect of dna lesions, which in vivo are potentially mutagenic, on in vitro dna synthesis carried out by a number of purified dna polymerases using a 0x174 template. both acetyl aminofluorene (aaf) adducts and uv-induced pyrimidine dimers are blocks to elongation by dna polymerases. on uv-irradiated dna templates synthesis terminates one nucleotide before the sites of pyrimidine dimers with all of the enzymes tested: pol i and pol iii holoenzyme from escherichia coli, t4 dna ... | 1982 | 6214248 |
| effect of bacterial host repair systems on the viability of hydroxylamine and methyl methanesulfonate treated t4 and lambda bacteriophages. | survival of ha1 or mms-treated t4 and lambda phages was estimated in bacterial cells differing in their ability to repair dna. it has been found that the mismatch repair system of the bacterial host, which involves muts mutr mutl uvre and dam loci, does not excise, or does so to only a limited extent, the nonpaired bases from dna of ha or mms-treated phages. mutation in pola, both in the polymerase as well as in the 5' leads to 3' exonuclease activity, have a small effect on survival of ha-treat ... | 1982 | 6214698 |
| secretion rates of thyroxine, triiodothyronine, and reverse triiodothyronine in man during surgery. | the secretion rates of t4, t3, and rt3 were studied in experiments of short duration by a new method based on determinations of the hormone difference across the thyroid combined with simultaneous electromagnetic thyroid blood flowmetry during surgery in 70 euthyroid patients. the secretion rate of t3 was similar in normal thyroid tissue and nodular goitre, but those of t4 and rt3 were lower in nodular goitre and solitary adenoma (p less than 0.05). in 61 patients with normal thyroid tissue or n ... | 1982 | 6215812 |
| the role of dna polymerase in base substitution mutagenesis on non-instructional templates. | in vitro dna synthesis on phi x174 or m13 templates with non-instructional lesions such as uv dimers or ap (apurinic/apyrimidinic) sites terminates one base before the site of the lesion when synthesis is catalyzed by t4 dna polymerase or e. coli polymerase i. e. coli polymerase i also produces termination bands at the site of ap lesions. substitution of mn2+ for mg2+ and increasing the concentration of dntp's results in elongation of the newly synthesized strand opposite the site of the lesion ... | 1982 | 6215955 |
| cell-mediated cytotoxicity against hla-d-region products expressed in monocytes and b lymphocytes. iv. characterization of effector cells using monoclonal antibodies against human t-cell subsets. | the cytotoxic effector cells that recognize hla-d-region determinants and their precursors were characterized using monoclonal antibodies against human t lymphocytes and t-cell subsets. these studies were performed using mlc combinations giving rise to cytotoxic cells specific for both class i (hla-a, b, c) and class ii (hla-d-region) antigens, and then tested against target cells displaying relevant antigens of only one class. both class i and class ii specific ctl (cytotoxic t lymphocytes) wer ... | 1982 | 6216209 |
| allospecific human t cell lines and clones which mediate hla-dr restricted helper activity. | in order to analyze the immunoregulatory activity of allospecific human t cells, we have combined the techniques of limiting dilution culture and il-2 dependent t cell growth to generate cloned alloreactive t cell lines (tcl). these tcl have been expanded in continuous culture for greater than 8 mo with retention of stable phenotypic and functional characteristics. for example, phenotypic analysis of alloproliferative tcl, utilizing a panel of monoclonal antibodies, demonstrate that these cultur ... | 1982 | 6216282 |
| effects of streptozotocin-induced diabetes mellitus on hypothalamic-pituitary-thyroid axis in rats. | the effects of streptozotocin-induced diabetes mellitus on the hypothalamic-pituitary-thyroid axis in rats were studied. streptozotocin (60 mg/kg) was injected ip. rats were decapitated at two and four weeks after the streptozotocin treatment. thyrotropin releasing hormone (trh), thyrotropin (tsh), thyroxine (t4), 3,3',5-triiodothyronine (t3), 3,3',5'-triiodothyronine (rt3), 3,3'-diiodothyronine (3,3'-t2) and 3',5'-diiodothyronine (3',5'-t2) were measured by means of the specific radioimmunoassa ... | 1982 | 6222895 |
| retardation of thyroxine-induced metamorphosis by amphenone b in toad tadpoles. | the effect of amphenone b, an inhibitor of corticoid synthesis, on thyroxine (t4)-induced metamorphosis was studied in toad tadpoles kept in thiourea. amphenone injections retarded t4-induced tail resorption markedly. the effect of amphenone was nullified by aldosterone and corticosterone added to the water in which tadpoles were kept. steroidogenic cells of adrenals in amphenone-injected animals were enlarged markedly as compared with those in the saline-injected tadpoles or the amphenone-injec ... | 1982 | 6221924 |
| [computed tomographic staging of prostatic cancer]. | 77 patients with carcinoma of the prostate (stage t1 = 4, stage t2 = 17, stage t3 = 28, stage t4 = 28) were investigated by computerized tomography for staging. intracapsular stage t1 tumors could not be identified as such. about 50% of clinical stage t2 tumors were shown to be of stage t3 by ct, whereas one fifth of the clinical stage t3 tumors were overestimated as demonstrated by ct. the extension of stage t4 tumors was more accurately depicted by ct then by clinical investigation. inspite of ... | 1982 | 6218044 |
| tumor-promoting phorbol esters selectively abrogate the expression of the t4 differentiation antigen expressed on normal and malignant (sézary) t helper lymphocytes. | 12-o-tetradecanoylphorbol-13-acetate (tpa) selectively abrogates, in nanomolar concentrations, the expression of the t4 differentiation antigen, as defined by the monoclonal anti-t4 antibody (1) on il-2-producing t helper lymphocytes from normal donors and on t helper lymphocytes from a patient with malignant t cell lymphoma (sézary's syndrome); this compound does not affect the expression of cell surface antigens as defined by the antibodies anti-okt3, -t6, -t8, -t11, ia, or anti-surface immuno ... | 1982 | 6218216 |
| [homology of lysozymes of bacterial and vertebrate origin]. | theoretical analysis of structural and functional organization of vertebrate lysozymes, t4-phage lysozyme, lambda-phage endolysin and extracellular lysozyme of chalaropsis species suggests a genetic relationship between the enzymes in question. it has been shown that the lysozyme sequences exhibit both inter- and intramolecular homology. the obtained data lend support to the concept postulating a common ancestor for the lysozyme family and subsequent divergent evolution of these proteins. the tw ... | 1982 | 6218392 |
| enrichment (and depletion) of human suppressor cells with monoclonal antibodies and immunoglobulin-coated plates. | a cell separation method using immunoglobulin (ig)-coated plates, originally devised for murine spleen cells, was modified and adapted for enrichment (and depletion) of cellular subpopulations from human peripheral blood. for the direct separation of b and t cells, f(ab')2 fragments of anti-human ig were used to coat the plates. for indirect separation, the cells were first incubated with monoclonal antibodies to cell surface antigens and then separated in plates coated with anti-mouse ig. plate ... | 1982 | 6216290 |
| [effect of freezing modes on the survival of escherichia coli bacteriophages]. | the object of this work was to study the effect of freezing down to--196 degrees c at different cooling and warming rates on the survival of t3, t4 and phix174 phages. phage particles survived when t3 phage was frozen at a rate of 20-400 degrees/min and phix174 phage at a rate of 20-45 degrees/min. the survival rate of t4 phage was highest when it was frozen at a rate of 45 degrees/min. the survival of the phages depended also on the regime of warming. the susceptibility of the phages to freezin ... | 1982 | 6216396 |
| excision of apurinic sites from dna with enzymes isolated from rat-liver chromatin. | apurinic sites were excised from phi x174 rf dna with two enzymes isolated from rat liver chromatin: an apurinic/apyrimidinic endodeoxyribonuclease and a 5'-3'-exonuclease; the resulting gap was filled with dna polymerase beta also prepared from rat liver chromatin and the repair was fully terminated with t4 ligase. | 1982 | 6217070 |
| [inhibition of the biosynthesis of thyroglobulin with propranolol in the rat]. | thyroglobulin biosynthesis was studied in thyroid glands of rats treated during 30 days with a daily dose of propranolol, a non-selective beta-adrenergic blocking drug. studies were carried out by extraction of soluble proteins from homogenates after incubation of the glands in presence of [3h]-4,5-l-leucine and [3h]-d-1-galactose. thyroglobulin 19s, 12s and 4-8s soluble proteins were separated and identified by ultracentrifugation in a saccharose gradient. the glands of rats treated with propra ... | 1982 | 6220762 |
| [repercussions of anterior hypothalamic lesions on nycthemeral variations of plasma thyroxine and corticosterone in the pigeon]. | plasma thyroxine and corticosterone levels were determined by competitive protein binding assay, at 3 hr intervals, throughout the photoperiod. pigeons were kept in controlled environment (21 +/- 1 degree c; 14l6-20: 10d). intact controls exhibited low thyroxine (t4) and corticosterone (b) levels for the light phase of the photoperiod. values were rising during the night, up to a peak at 03 hr. electrolytic lesions were placed bilaterally in either the nucleus anterior medialis hypothalami or th ... | 1982 | 6220770 |
| [effects of exogenous tsh on the thyroid activity of adult or neotenic amphibians]. | in adult anuran and neotenic urodela, bred in laboratory conditions, the levels of plasma thyroid hormones are undetectable (t3 less than 50 ng/100 ml, t4 less than 5 ng/100 ml). thyroid function can be reactivated after ovine tsh treatment. under those conditions, metamorphosis is induced in the axolotl and t4 plasmatic levels reaches 0,53 +/- 0,13 micrograms/100 ml and those of t3 9 +/- 2,64 ng/100 ml. in adult anuran, thyroid reactivation under thyrotropic treatment determines an increased se ... | 1982 | 6220788 |
| thyroid function in a diabetic population. | several alterations in thyroid function are found in diabetes mellitus (see table i). the most profound changes occur in patients with insulin-dependent diabetes. plasma t4 is normal whereas plasma t3 is diminished, and the plasma level of rt3 is elevated in diabetic ketoacidosis or in patients with severely uncontrolled diabetes. these changes arise from alterations in the monodeiodination pathways of t4. both hypo- and hyperthyroidism occur with increased frequency in diabetes. there is an inc ... | 1982 | 6302931 |
| determination of the amount of homology required for recombination in bacteriophage t4. | homology is an important feature of recombination. we have used the rll cistrons of bacteriophage t4 to determine the extent of homology required for recombination. we varied the amount of homologous dna available for recombination in both marker rescue experiments and deletion-by-deletion crosses. our results suggest that the primary pathway for recombination in t4 requires 50 bp of homology. our finding that recombination is detectable when fewer than 50 bp of homology are available suggests t ... | 1982 | 6297750 |
| prevention of propranolol withdrawal mechanism by prolonged small dose propranolol schedule. | abrupt withdrawal of propranolol may be followed by a "propranolol withdrawal syndrome "due, at least in part, to enhanced beta adrenergic sensitivity. tapering propranolol dosage is frequently used in the hope of preventing adverse withdrawal events but the success of such a maneuver has not been shown. the rationale for the dose tapering schedule in this study was based on earlier observations after abrupt withdrawal of propranolol. nine hypertensive patients were gradually withdrawn from long ... | 1982 | 6278914 |
| characterization of an automated radioimmunoassay for t4, t3, t3 u, and fti. | the performance characteristics of assays is reported for thyroxine (t4), triiodothyronine (t3), and t3-uptake (t3u) using the gammaflotm automated assay system. a comparison of calculated free thyroxine index (fti) values is also presented. this automated radioimmunoassay (ria) system utilizes a combination of continuous-flow methodology and chromatographic separation techniques. the t4 assay studied had a standard curve range of 1.5 to 24.0 microgram per dl. the intra- and inter-assay precisio ... | 1982 | 6279016 |
| bacteriophage t4-induced anticodon-loop nuclease detected in a host strain restrictive to rna ligase mutants. | the fate of host trnas during t4 bacteriophage infection was investigated with escherichia coli ctr5x, the only known host strain that is restrictive to rna ligase and polynucleotide kinase mutants. three ctr5x trna species were cleaved during infection. one was leucine trna1, which was cleaved in the extra arm, as reported elsewhere for e. coli b infected with bacteriophage t2 or t4. the other two were specific to e. coli ctr5x and were not cleaved in various other hosts. one of the cleaved ctr ... | 1982 | 6296815 |
| [diagnostic reliability of scintigraphy in hepatic metastases of breast cancer]. | the authors have reviewed their experiences in determining the presence of liver metastases in 103 patients by radiocolloid scanning. the sensitivity of liver scanning proved to be quite low if the presence of focal defects of tracer's distribution was chosen as the diagnostic criterion. the inclusion of less restrictive criteria, such as liver enlargement or irregular distribution of the tracer, resulted in an higher sensitivity without lowering the predictive value of a negative scan. using th ... | 1982 | 6296928 |
| [endocrine reaction pattern in the course of a one-phase tramadol-n2o combination anesthesia]. | the effects of tramadol-n2o-anaesthesia on the per- and postoperative change in blood concentrations of cortisol, prolactin, thyroxine, triiodothyronine, cyclic amp, glucagon, antidiuretic hormone, pth-peptide (44-68), glucose, lactate, pyruvate and free fatty acids (ffa) were investigated in connection with elective orthopaedic surgery. anaesthesia in man with tramadol and nitrous oxide were found to be associated with a significant elevation of plasma cortisol and plasma prolactin in man. howe ... | 1982 | 6297329 |
| viricidal capability of resin-triiodide demand-type disinfectant. | polyoma, newcastle disease virus, and adenovirus, as well as two coliphages, lambda and t4, were inactivated by strong base quaternary ammonium anion-exchange resin-triiodide. organic matter interfered with viral inactivation capability of the resin-triiodide. the viruses, as they were being inactivated by the resin disinfectant beads, were not retained or filtered by the beads. | 1982 | 6297389 |
| impairment of renal compensatory hypertrophy by hypothyroidism in the rat. | renal compensatory hypertrophy (rch) occurs in hypothyroid rate, but it is impaired when compared to rch found in euthyroid controls. it is due to cellular hypertrophy as the dna content does not change and the protein/dna ratio increases in the compensating kidney. rch is enhanced by thyroxine (t4) with a rise in the dna content of the compensating kidney, but the protein/dna ratio does not change indicating that hypertrophy is as important as hyperplasia. corticotrophin (acth) given to eu and ... | 1982 | 6280010 |
| studies on the mechanism of thyroid hormone stimulation in vitro of human red cell ca2+-atpase activity. | the stimulation in vitro of human red blood cell ca2+-atpase activity by thyroxine (t4) and triiodothyronine (t3) in physiological concentrations is shown to depend upon binding of iodothyronines to red cell membranes. calmodulin enhances the activity of thyroid hormone in this model system but there is no direct interaction of calmodulin and hormone. | 1982 | 6280012 |
| digestion of highly modified bacteriophage dna by restriction endonucleases. | the ability of thirty type ii restriction endonucleases to cleave five different types of highly modified dna has been examined. the dna substrates were derived from relatively large bacteriophage genomes which contain all or most of the cytosine or thymine residues substituted at the 5-position. these substituents were a proton (pbs1 dna), a hydroxymethyl group (sp01 dna), a methyl group (xp12 dna), a glucosylated hydroxymethyl group (t4 dna), or a phosphoglucuronated, glucosylated 4,5-dihydrox ... | 1982 | 6280151 |
| low protein-high carbohydrate diet induces alterations in the serum thyronine-binding proteins in the rat. | the serum t3 concentration was increased in 8-week-old lean zucker rats fed a low protein-high carbohydrate diet for 2 weeks. this increase was secondary to the generation of a binding protein migrating in the postalbumin zone in polyacrylamide gel electrophoresis employing 125i-labeled t3 and is termed rat thyronine-binding globulin. the presence of this t3-binding protein in serum resulted in a marked decrease in the percent free t3 assessed by equilibrium dialysis and a normal free t3 concent ... | 1982 | 6280968 |
| nucleic binding affinity of bacteriophage t4 gene 32 protein in the cooperative binding mode. | this study reports on various parameters which affect the binding stoichiometry for complexes of bacteriophage t4 gene 32 protein (p32) and single stranded polynucleotides (determined by uv absorbance and fluorescence quenching) and presents results of a quantitative electron spin resonance assay to determine physiologically effective binding affinity differences of nucleic acid binding proteins. the assay employs macromolecular spin probes (spin-labeled nucleic acids) which are used to determin ... | 1982 | 6281260 |
| transcriptional initiation and processing of the small ribosomal rna of yeast mitochondria. | we have identified the nucleotide at which transcription initiates on the yeast mitochondrial small (14 s) rrna gene by sequencing of rna labeled at the 5' initiating triphosphate with vaccinia virus guanylyltransferase [alpha-32p]gtp (in vitro capping reaction). initiation occurs within the stem of a 12-base palindromic repeat. the initiation sequence has homology with the large (21 s) ribosomal rna initiation sequence that has been previously determined. we have also sequenced the 5' and 3' en ... | 1982 | 6281273 |
| investigations into the etiology of elevated serum t3 levels in protein-malnourished rats. | thyroid function studies and the peripheral metabolism of thyroid hormone were examined in rats fed a low protein diet (9% casein) for 4-8 wk. compared to animals fed a normal protein diet ad libitum, both the low protein rats and a pair-fed control group weighed less at the end of the study. however, serum total t3 levels were significantly higher only in the protein deficient rats. the elevated serum t3 was not explainable by enhanced peripheral t4 to t3 conversion, as there was no evidence of ... | 1982 | 6281617 |
| a comparison of the phage t4 gene 32 protein and escherichia coli rna polymerase binding sites on hamster papovavirus dna. | phage t4 gene 32 protein and escherichia coli rna polymerase were bound to hamster papovavirus dna. the binding regions were identified by electron microscopy employing a protein-free spreading technique. after gene 32 protein treatment four denaturation regions could be mapped, at 0.04-0.12, 0.30-0.36, 0.50-0.60 and 0.75-0.90 dna map units, respectively, using the unique bamhi cleavage site as zero point. eight rna polymerase binding sites can be found which are localized at positions 0.05; 0.1 ... | 1982 | 6282329 |
| computed tomography of malignant tumors of the nasal cavity and paranasal sinuses. | staging of malignant tumors of the nasal cavity and paranasal sinuses by computed tomography (ct) was studied in a total of 49 patients, 33 with squamous cell carcinoma and 16 with tumors of other histologic types. involved sites by the tumor were studied, and clinical staging was made using ct findings alone according to ajc classification for maxillary sinus tumors. surgical findings for comparison were available for most cases. of 33 squamous cell carcinomas and of 16 tumors with other histol ... | 1982 | 6282436 |
| template-prime-dependent turnover of (sp)-datp alpha s by t4 dna polymerase. the stereochemistry of the associated 3' goes to 5'-exonuclease. | t4 dna polymerase converts (sp)-2'-deoxyadenosine 5'-o-(1-thio[1-18o2]triphosphate) to 2'-deoxyadenosine 5'-o-[18o]-phosphorothioate in the presence of poly(d(a-t).poly(d(a-t)) template-primer. control experiments involving either omitting the poly(d(a-t)).poly(d(a-t) template-primer or employing the (rp)-2'-deoxyadenosine 5'-o-(1-thiotriphosphate) diastereomer showed no reaction. it is assumed, therefore, that this conversion as in the p--o case involves incorporation of the thionucleotide into ... | 1982 | 6282851 |
| the gol site: a cis-acting bacteriophage t4 regulatory region that can affect expression of all the t4 late genes. | 1982 | 6283094 | |
| ligation of oligonucleotides by pyrimidine dimers--a missing 'link' in the origin of life? | one of the principal photochemical reactions of dna on exposure to uv is the formation of intrastrand cyclobutane-type pyrimidine dimers. the efficiency of this reaction depends on both the wavelength of the uv2 and the specific nucleotide sequence in the dna. the formation of the pyrimidine dimer and its repair in living cells have been studied extensively. we have examined the possibility that pyrimidines at the ends of dna strands may be adequately juxtaposed for dimer formation by the presen ... | 1982 | 6283388 |
| thyroid hormone binding to putative nuclear receptors in human mononuclear blood cells. | nuclear protein extracts from mononuclear cells in normal subjects, subjects with the clinical syndrome of thyroid hormone resistance and cultured b lymphocytes bound thyroxine (t4) ka; 1 . 3-3 . 5 x 10(9) m-1) and triiodothyronine (t3) (ka; 1 . 2-3 . 9 x 10(9) m-1) with similar affinities. when an extra wash step was introduced, the maximum specific binding of t4 and t3 was reduced by 75% and binding was abolished by three washes. these data suggest binding is to a serum protein contaminant pre ... | 1982 | 6284102 |
| bacteriophage t4 gene 45. sequences of the structural gene and its protein product. | bacteriophage t4 gene 45 codes for a protein whose functions are required for both t4 dna replication and t4 late gene transcription. to facilitate studies of the interactions of 45 protein with the t4 dna replication complex and with rna polymerase, we have determined the primary structure of 45 protein. the amino acid sequence of 45 protein has been determined by correlating nucleotide sequence analysis of gene 45 with protein chemistry studies of 45 protein. our studies indicate that gene 45 ... | 1982 | 6284751 |
| thyroid-stimulating hormone (tsh) receptor antibodies and antibodies stimulating adenyl cyclase in relatives from two families with a high prevalence of graves' hyperthyroidism: a ten-year follow-up study. | thyroid-stimulating hormone (tsh) receptor antibodies and antibodies stimulating adenyl cyclase were measured in 47 relatives of patients with graves' hyperthyroidism from two families with a high prevalence of the disease, in whom bioassays for the long-acting thyroid stimulator (lats) had been performed 10 years earlier. tests were also carried out in six propositi from the two families and age- and sex-matched normal subjects from six families. there had been no new cases of hyperthyroidism s ... | 1982 | 6284789 |
| ontogeny of beta-adrenergic receptors in the rat lung: effects of hypothyroidism. | beta-adrenergic receptors were identified in membrane fractions of rat lung with the beta-adrenergic antagonists (-)-[3h]-dihydroalprenolol (-)-[3h]dha) and (+/-)-[125i]-iodohydroxybenzylpindolol ((+/-)-[125i]hyp). binding capacity (bmax) for (-)-[3h]dha increased progressively from 46 +/- 7 on day 18 of gestation to 510 +/- 70 femtomoles . mg-1 protein (mean +/- s.d.) on postnatal day 28, at which time adult bmax was attained. an increase in (-)-[3h]dha binding capacity of the lung was observed ... | 1982 | 6285262 |
| beta-adrenergic receptors and catecholamine sensitive adenylate cyclase in developing rat ventricular myocardium: effect of thyroid status. | the present study was designed to describe the relationships among thyroid status, myocardial growth and myocardial beta-adrenergic receptors in the developing rat ventricle. in normal rat myocardium the beta-adrenergic binding capacity (bmax) for (-)-[3h] dha decreased with increasing age and heart size. in order to determine the effect of thyroid status on ventricular growth characteristics and beta-adrenergic receptors, animals were rendered: (1) hypothyroid with propylthiouracil (ptu), (2) e ... | 1982 | 6285264 |
| congenital secondary hypothyroidism with low serum gh and prolactin levels in a 27-day-old male infant. | a male infant with secondary hypothyroidism is described. within the first month after birth, the patient manifested feeding difficulties, lethargy, persistent jaundice, umbilical hernia, and large anterior and open posterior fontanels. the roentgenogram of the knee joints at 27 days showed absence of the distal femoral epiphyses. his serum thyroid-stimulating hormone (tsh) level was low despite decreased levels of triiodothyronine (t3) and tetraiodothyronine (t4) in serum. assessment of the hyp ... | 1982 | 6285548 |
| cloning of uvsw and uvsy genes of bacteriophage t4. | 1982 | 6285592 | |
| lack of stimulation of renal (na+ +k+)-atpase by thyroid hormones in the rabbit. | the effect of 1-3,5,3'-triiodothyronine (t3) and thyroxine (t4) on (na+ +k+)-atpase activities was examined in rabbit kidneys because in this tissue almost 80% of the metabolism is connected to active sodium transport. t3-receptor concentrations were estimated as 0.62 and 0.80 pmol/mg per dna in the cortex and outer medulla, respectively. a dose of 0.5 mg t3/kg body weight for 3 days increased basal metabolic rate by almost 60%, and the mitochondrial 1-alpha-glycerophosphate dehydrogenase activi ... | 1982 | 6285989 |
| [endemic goiter in the man region (ivory coast)]. | an epidemiological study involving 616 subjects carried out in the man region, côte-d'ivoire, showed endemic goitre in 54,4% of the group overall and in 80 % of females. thyroid function of subjects chosen at random in the goitrous and nongoitrous group (105 g and 71 ng respectively) was identical. compared with a french control group t4 was significantly lower but t3 and tsh were higher, which represents a high tbg level with low iodine elimination. high levels of tsh are not necessarily associ ... | 1982 | 6286160 |
| postnatal action of growth and thyroid hormones on the retarded cerebral myelinogenesis of snell dwarf mice (dw). | snell dwarf mice (dw) showed a lower cnpase activity (59% of the normal controls) only in the cerebrum among different parts of the cns, and a strikingly reduced level of spontaneous locomotion activity with an indistinct diurnal periodicity in a 24-h record at 40 days of age. daily administration of bgh and t4 to the dwarfs during the first 40 days of postnatal life restored cnpase activity to the level of the normal controls, and was accompanied by normalization of the pattern of spontaneous l ... | 1982 | 6286878 |
| dna glycosylases. | various dna glycosylases exist, which initiate the first step in base-excision repair. a summary of the kinetic and physical characteristics of three classes of dna glycosylases are presented here. the first class discussed, include glycosylases which recognize alkylated dna. various data from enzymes derived from both prokaryotic and eukaryotic sources is discussed. the second class deals with a glycosylase that recognizes and initiates the excision of pyrimidine dimers in dna. to date, this en ... | 1982 | 6287206 |
| dna precursors in chemical mutagenesis: a novel application of dna sequencing. | recently, we have shown that deoxyribonucleoside residues in the cellular dna precursor pool are generally more susceptible to methylation than are residues within the dna duplex. the n-1 position of adenosine, for example, was found to be at least 13,000 times more susceptible to methylation by n-methyl-n-nitrosourea (mnu) than the same site in the dna. these results suggest that potential sites for alkylation in the double-strand duplex are relatively inaccessible to direct alkylation in vivo. ... | 1982 | 6287277 |
| t4 late transcripts are initiated near a conserved dna sequence. | bacteriophage t4 late transcription is unusual, among prokaryotes, in its complexity. late transcription requires the host rna polymerase, the products of t4 genes, 33, 45 and 55, and other small polypeptides, the genes of which have not been identified. in addition the dna template must be "competent' for late transcription. first the dna must contain the substituted base 5-hydroxymethyl cytosine in place of cytosine (this requirement is eliminated by a mutation in the t4 alc gene). second, the ... | 1982 | 6287296 |
| enzymatic replacement in vitro of the first anticodon base of yeast trnaasp: application to the study of trna maturation in vivo, after microinjection into frog oocytes. | a combination of several enzymes, rnase-t1, nuclease s1, t4-polynucleotide kinase and t4-rna ligase were used to prepare and modify different fragments of yeast trnaasp (normal anticodon g u c). this allowed us to reconstitute, in vitro, a chimeric trna that has any of the four bases g, a, u or c, as the first anticodon nucleotide, labelled with (32p) in its 3' position. such reconstituted (32p) labelled yeast trnaasp were microinjected into the cytoplasm or the nucleus of the frog oocyte and ch ... | 1982 | 6287419 |
| familial elevations of total and free thyroxine in healthy, euthyroid subjects without detectable binding protein abnormalities. | elevations of serum thyroxine without thyrotoxicosis or binding protein abnormalities have been documented in 8 of 13 family members, representing 4 generations. this syndrome appears to represent an elevated threshold for the amount of free thyroxine substrate required to maintain adequate t3 production form the peripheral monodeiodination of t4. it reiterates the need for a prudent re-evaluation of all clinically euthyroid patients with elevated serum thyroxine concentrations before concluding ... | 1982 | 6287778 |
| single-stranded gaps as localized targets for in vitro mutagenesis. | short single-stranded gaps in circular dna molecules can be generated enzymatically, often at predetermined sites. these can serve as targets for in vitro mutagenesis procedures that result in alterations in nucleotide sequence within or very near the gap. deamination of unpaired cytosine residues with sodium bisulfite has been used to induce mutations in the bgli restriction site of sv40 dna and within defined regions of the beta-lactamase gene on pbr322. a new method of induction of mutations ... | 1982 | 6287991 |
| t4 endonuclease vii cleaves holliday structures. | t4 endonuclease vii cleaves holliday structures in vitro by cutting two strands of the same polarity at or near the branch point. the two unbranched duplexes produced by cleavage each contain a strand break that can be sealed by dna ligase. this suggests that the cut sites are at the same position in the nucleotide sequence in each strand. the joint action of endonuclease vii and dna ligase can therefore resolve holliday structures into genetically sensible products. these observations account f ... | 1982 | 6288255 |
| adrenal innervation may be an extrapituitary mechanism able to regulate adrenocortical rhythmicity in rats. | the daily rhythm of plasma corticosteroid concentration was studied in nonhypophysectomized rats and in hypophysectomized rats implanted with beeswax pellets containing acth and t4. in the first experiment, male hypophysectomized rats were placed on a 12-h light, 12-h dark cycle (onset of light, 0600 h) with food and water available ad libitum. beeswax pellets containing 1.5 mg acth and 150 micrograms t4 were implanted sc in these rats. beginning 4 days after the implantation of acth and t4, dai ... | 1982 | 6288356 |
| isolation and characterization of two mutant forms of t4 polynucleotide kinase. | the purification of polynucleotide kinase from escherichia coli infected by two different mutants in the t4 polynucleotide kinase (pset) gene is described. the pset 1 enzyme has virtually no 3' specific phosphatase activity and normal polynucleotide kinase activity. the pset 47 enzyme has very little phosphatase activity and no kinase activity. however, enzyme isolated from a pset 1, pset 47 mixed infection appears to contain heterodimers with considerably more phosphatase activity. thus, the ps ... | 1982 | 6288679 |
| independent locations of kinase and 3'-phosphatase activities on t4 polynucleotide kinase. | we have used two chemical modification reagents and three proteases to study the relationship between the two activities of t4 polynucleotide kinase. in each case, conditions were found where one of the two activities of the enzyme could be eliminated without greatly reducing the other. taken together, these data indicate that the two activities are catalyzed by amino acid residues located in separate active sites on the polypeptide chain. specific exopeptidase digestion indicates that the kinas ... | 1982 | 6288680 |
| bacteriophage t4 gene 41 protein, required for the synthesis of rna primers, is also a dna helicase. | bacteriophage t4 gene 41 protein is one of the two phage proteins previously shown to be required for the synthesis of the pentaribonucleotide primers which initiate the synthesis of new chains in the t4 dna replication system. we now show that a dna helicase activity which can unwind short fragments annealed to complementary single-stranded dna copurifies with the gene 41 priming protein. t4 gene 41 is essential for both the priming and helicase activities, since both are absent after infection ... | 1982 | 6288720 |
| cea positivity in sera and breast tumor tissues obtained from the same patients. | it is well known that the concentration of serum cea correlates best with colorectal carcinomas but less well with other solid tumors. the aim of the present study was to elucidate further the poor relationship between confirmed primary mammary carcinomas and cea serum levels, 108 of these tumors have hence been studied for their tissular cea positivity and the results obtained were compared with the cea serum values found preoperatively in the same patients. techniques employed were the indirec ... | 1982 | 6289290 |
| nucleotide sequences involved in bacteriophage t4 gene 32 translational self-regulation. | we have determined the nucleotide sequence of a cloned segment of the bacteriophage t4d chromosome, which contains the regulatory sequences and the structural gene (gene 32) for the single-stranded dna binding protein (gp32). the amino acid sequence predicted by translation of the structural gene agrees well with that published for gp32 [williams, k. r., lo-presti, m. b., setoguchi, m. & konigsberg, w. h. (1980) proc. natl. acad. sci. usa 77, 4614-4617]. to localize the nucleotide sequence invol ... | 1982 | 6289325 |
| resistance to thyroid hormones. a disorder frequently confused with graves' disease. | five patients from two unrelated families were found to have goiter and elevated serum concentrations of thyroxine (t4) and triiodothyronine (t3) without symptoms or signs of hyperthyroidism. all had measurable concentrations of thyroid stimulating hormone (tsh), and in four who were tested, there was an increase in tsh concentration following thyrotropin releasing hormone (trh) administration. we believe these five patients have general resistance to the effects of thyroid hormones and need ele ... | 1982 | 6289763 |
| ligation of restriction endonuclease-generated dna fragments using immobilized t4 dna ligase. | 1982 | 6289831 | |
| photoaffinity labelling of the rat liver nuclear thyroid hormone receptor with [125i]triiodothyronine. | [125i]triiodothyronine (t3) was used as a photoreactive probe for the thyroid hormone nuclear receptor in photoaffinity labelling experiments. autoradiograms of photolysis products electrophoresed on either one or two-dimensional gels showed that [125i]t3 covalently, but nonspecifically, labelled many proteins in the partially purified receptor preparations used. however, one of these proteins with an estimated molecular weight of 47,000 and an isoelectric point of approximately 6.2 +/- 0.5 ph u ... | 1982 | 6290199 |
| a case with isolated acth deficiency accompanying chronic thyroiditis. | an unusual case of isolated acth deficiency with coexisting chronic thyroiditis in a 53-year-old man is reported. the patient was admitted with a 2-year history of generalized fatigue, a 13-kg weight loss, muscular weakness, and frequent hypotensive and hypoglycemic attacks. on admission serum thyroxine and triiodothyronine were significantly elevated. basal tsh concentration was not detected and tsh showed no response to trh, but one month after replacement therapy with hydrocortisone it was sh ... | 1982 | 6290200 |
| bacteriophage t4 as a generalized dna-cloning vehicle. | we have designed a method for inserting foreign dna segments into bacteriophage t4. a plasmid containing t4 dna is opened within the t4 sequence and the foreign dna is inserted in vitro. recombination in vivo, between t4 and the doubly chimeric plasmid, results in insertion of the foreign dna into the genome of viable t4 phage. we have demonstrated the method by inserting a 203-bp dna fragment from the lactose operon of escherichia coli, into the dispensable region of the riib gene of t4. with m ... | 1982 | 6290333 |
| cytochrome c reduction by triiodothyronine (t3). | triiodothyronine (t3), the active thyroid hormone, reduced cytochrome c non-enzymatically. this reduction was partially inhibited by superoxide dismutase (sod). thyroxine (t4) was only minimally effective as a reductant in this system. the effect of t3 on mitochondrial oxidative phosphorylation may involve a direct redox mechanism. | 1982 | 6290858 |
| [treatment of inflammatory breast cancer. controlled study of a combination therapy program]. | the authors report 27 cases of inflammatory carcinoma of the breast (t3-t4, m0) and propose: induction chemotherapy (adriamycin, vincristin, methotrexate) conventional local and regional radiotherapy, sometimes complementary surgical exeresis, and a complement of consolidation chemotherapy (cyclophosphamid, methotrexate, fluoro-uracil or adriamycin, vincristin, and methotrexate). in these forms, where the prognosis is very bad, this therapeutic regimen may improve considerably the median remissi ... | 1982 | 6291165 |
| thyroid hormone administration in vivo regulates the activity of hepatic glycogen phosphorylase phosphatase. | short term (48 h) administration in vivo of either t4 or t4, but not the biologically inactive d-isomer of t3, was associated with a decrease in basal glycogen phosphorylase alpha activity and an increase in phosphorylase alpha phosphatase activity of rat hepatocytes. this influence of thyroid hormones on hepatic phosphorylase alpha and phosphorylase phosphatase activities was shown to be dose dependent. as little as 0.0025 mg t3/kg bw administered in vivo at 48, 24, and 3 h before liver excisio ... | 1982 | 6291901 |
| effects of hormones on mammary adenosine 3',5'-monophosphate levels and metabolism in normal and adrenalectomized lactating rats. | prostaglandin e1 (pge1) t4, and epinephrine (epi) were incubated with mammary tissue slices from normal and adrenalectomized (adx) rats and adx rats receiving cortisol (adx + c). pge1, t4, and the combination of epi, t4, and pge1 increased mammary slice camp levels in all groups. no differences were observed among treatment groups in basal camp levels or responses to hormones. glucose-1-14c oxidation and incorporation into lipids were stimulated by pge1, t4, and the combination of epi, t4, and p ... | 1982 | 6291908 |
| a family of cloning vectors containing the lacuv5 promoter. | a family of plasmids containing short pieces of escherichia coli lac promoter dna has been constructed. dna fragments from any source may be inserted directly into the unique ecori sites of some of these plasmids to achieve transcription under the control of the lacuv5 promoter. alternatively, the plasmids serve as convenient sources of lac dna fragments ('portable promoters') containing the 'up' promoter mutations uv5 or ps (super promoter) as well as the wild-type promoter. pop95-2, pop95-5, p ... | 1982 | 6292048 |
| herpes simplex virus infection of human t-cell subpopulations. | the ability of herpes simplex virus type 1 to productively infect human t-cell subpopulations was examined. unstimulated helper/inducer (t4+) and cytotoxic/suppressor (t8+) lymphocytes limited herpes simplex virus replication as effectively as unseparated peripheral blood t cells (t3+). phytohemagglutinin stimulation before infection resulted in equivalently productive herpes simplex virus infections in the three cell fractions. | 1982 | 6292113 |
| effect of sustained-release theophylline administration on pituitary-thyroid axis. | the effect of therapeutic doses of theophylline on the pituitary-thyroid axis was studied in four normal adults and in 15 asthmatic children on theophylline treatment. in the four normal adults, sustained-release theophylline was administered every 12 hr for 60 hr, and serial determinations of serum theophylline, thyroxine (t4), triiodothyronine (t3), reverse triiodothyronine (rt3), thyrotropin (tsh), and cyclic amp (camp) were done. in all four cases, serum t4 increased significantly (134% +/- ... | 1982 | 6292280 |
| the diagnostic application of radiocolloid liver scintigraphy in breast carcinoma. | the authors have reviewed their experiences in determining the presence of liver metastases in 103 patients by radiocolloid scanning. the sensitivity of liver scanning proved to be quite low if the presence of focal defects in the distribution of the tracer was chosen as the diagnostic criterion. the inclusion of less restrictive criteria such as liver enlargement or irregular distribution of the tracer, resulted in a higher sensitivity without lowering the predictive value of a negative scan. u ... | 1982 | 6292871 |
| post-excitatory depression in thoracic sympathetic efferent neural traffic during a cardiogenic hypertensive chemoreflex. | serotonin injected in the left atrium activates a cardiogenic hypertensive chemoreflex in dogs. to elucidate patterns of the neural traffic, records were obtained from thoracic sympathetic efferent nerves (either the anterior ansa of the left stellate ganglion or the t4 input to the left stellate) in 8 anesthetized dogs with chest open. serotonin (200 micrograms, left atrium) caused a massive sympathetic discharge during the hypertension and bracardia characteristic of the chemoreflex. following ... | 1982 | 6293455 |
| photoaffinity labeling of human thyroxine-binding prealbumin with thyroxine and n-(ethyl-2-diazomalonyl)thyroxine. | to facilitate studies of thyroid hormone-binding proteins, we have synthesized and tested the photoaffinity analogues n-(ethyl-2-diazomalonyl)-3,5,3'-triiodothyronine (edm-t3) and n-(ethyl-2-diazomalonyl)thyroxine (edm-t4). the binding affinities of l-edm-t4 and d-edm-t4 to human thyroxine-binding prealbumin were 4% and 13.2%, respectively, that of l-thyroxine (l-t4). for comparison the affinities of l-edm-t3 and d-edm-t3 to crude rate liver nuclear receptor preparation were 0.1% and 0.7%, respe ... | 1982 | 6293553 |
| topoisomerase involvement in multiplicity reactivation of phage t4. | the products of phage t4 genes 39, 52 and probably 60 have been previously characterized as forming a type ii dna topoisomerase. other evidence suggested that this topoisomerase promotes normal initiation of dna replication, and that when it is defective its loss is partially compensated for by the host gyrase. we present evidence here that mutants defective in genes 39, 52 and 60 have reduced ability to carry out multiplicity reactivation (mr, a form of recombinational repair) of phage dna dama ... | 1982 | 6293912 |
| [gonadotropin and plasma cyclic nucleotides]. | plasma cyclic nucleotides in some physiological conditions were measured in normal women and the changes of cyclic amp were studied after the loading of gonadotropins in normal and castrated women. we demonstrated a circadian rhythm of cyclic amp and its variation during menstrual cycle in normal women and also demonstrated its significant increase in pregnant women. but there was no significant variation of plasma cyclic gmp levels in those physiological conditions. plasma cyclic amp was increa ... | 1982 | 6294197 |
| transcription in vitro of an isolated fragment of bacteriophage t4 genome. | 1982 | 6294307 | |
| on the role of the single-stranded dna binding protein of bacteriophage t4 in dna metabolism. i. isolation and genetic characterization of new mutations in gene 32 of bacteriophage t4. | the product of gene 32 of bacteriophage t4 is a single-stranded dna binding protein involved in t4 dna replication, recombination and repair. functionally differentiated regions of the gene 32 protein have been described by protein chemistry. as a preliminary step in a genetic dissection of these functional domains, we have isolated a large number of missense mutants of gene 32. mutant isolation was facilitated by directed mutagenesis and a mutant bacterial host which is unusually restrictive fo ... | 1982 | 6294482 |
| t4 bacteriophage-coded polynucleotide kinase and rna ligase are involved in host trna alteration or repair. | 1982 | 6294988 | |
| structure of the tyrosyl radical in bacteriophage t4-induced ribonucleotide reductase. | ribonucleotide reductase induced by bacteriophage t4 in escherichia coli contains an organic free radical necessary for enzymatic activity. its epr spectrum at 77k is similar to but not identical with that of the corresponding radical in the enzyme from uninfected e. coli studied previously. isotope substitutions now show that the radical in the t4-induced enzyme also is localized to a tyrosine residue with its spin density delocalized over the aromatic ring of tyrosine. the difference between t ... | 1982 | 6273437 |
| involvement of alpha 1-adrenergic receptors in the inhibitory effect of catecholamines on the thyrotropin-induced release of thyroxine by the mouse thyroid. | tsh enhanced the release of t4 from the mouse thyroid incubated in vitro. norepinephrine, a nonspecific alpha-adrenergic agonist, and methoxamine, an alpha 1-agonist, inhibited the tsh-stimulated release of t4 at 10(-4) and 10(-5) m, whereas clonidine, an alpha 2-agonist, exerted a weak inhibition. the inhibitory effect of 10(-5) m norepinephrine on the t4 release stimulated by tsh was prevented by prazosin, an alpha 1-antagonist, at concentrations higher than 10(-7) m, whereas yohimbine, an alp ... | 1982 | 6274625 |
| thyroxine binding globulin and thyroid function tests in patients with hepatocellular carcinoma. | to determine the prevalence of elevated serum concentrations of thyroxine binding globulin (tbg) in patients with hepatocellular carcinoma (hcc) and the influence of the associated cirrhosis, tbg was measured in 39 patients with hcc, 22 with and 17 without cirrhosis, in 20 patients with cryptogenic macronodular cirrhosis but without hcc, and in 40 matched controls. the mean serum tbg concentration in the patients was 34.5 +/- 17.7 microgram per ml, compared to 21.4 +/- 6.8 microgram pr ml in con ... | 1982 | 6274780 |
| physical mapping and cloning of bacteriophage t4 anti-restriction endonuclease gene. | we have proposed that the ability of t4 to produce non-glucosylated progeny after a single cycle of growth on a galu rgla rglb+ mutant of escherichia coli is due to the initiation of the rglb+ function by a phage-coded, anti-restriction endonuclease protein. based on this hypothesis, we screened t4 deletion mutants for failure to give a burst in this host. the absence of an arn gene in phage mutants lacking the 55.5- to 58.4-kilobase region is verified by their inability to protect secondary inf ... | 1982 | 6276366 |
| purification and characterization of normal and mutant forms of t4 endonuclease v. | endonuclease v of bacteriophage t4 has been purified to physical homogeneity from t4d-infected escherichia coli 1100. the enzyme, whose molecular weight is 16,000, possessed two distinct catalytic activities, a pyrimidine dimer-dna glycosylase and an apurinic/apyrimidinic endonuclease. they acted on uv-irradiated poly(da) . poly(dt) in a sequential manner; the glycosylase cleaved the n-glycosyl bond between the 5'-pyrimidine of a dimer and the corresponding sugar and then the endonuclease hydrol ... | 1982 | 6277906 |
| construction of a hybrid plasmid molecule containing the total coding region of semliki forest virus 26s mrna. | we have constructed a hybrid plasmid molecule that contains the complete coding sequences from the 26s mrna of semliki forest virus. five fragments which together covered the mrna sequence were isolated from three original hybrid plasmids and joined together at five different restriction endonuclease cleavage sites using t4 dna ligase. | 1982 | 6278065 |
| temperature dependence of the activity of dna-modifying enzymes: endonucleases and dna ligase. | the activities of 17 endonucleases: the restriction endonucleases avai, bam hi, ecori, hindiii, psti and sali, which cleave pbr322 dna once: alui, avaii, cfoi, haeiii, hhai, hinfi, hpaii and taqi, which cut pbr322 dna several times, and three 'unspecific' nucleases (s1 nuclease, staphylococcal nuclease and dnase i from bovine pancreas) were determined between 0 degrees and 65 degrees c. the reaction was followed by the disappearance of covalently closed circular pbr322 dna, using the alkaline et ... | 1982 | 6279395 |
| the topoisomerase activity of t4 amg39 mutant is restored in mu lysogens. | 1982 | 6279432 | |
| characterization of the free radical of mammalian ribonucleotide reductase. | mouse fibroblast 3t6 cells, selected for resistance to hydroxyurea, were shown to overproduce protein m2, one of the two nonidentical subunits of mammalian ribonucleotide reductase. packed resistant cells gave an epr signal at 77 k very much resembling the signal given by the tyrosine-free radical of the b2 subunit of escherichia coli ribonucleotide reductase. also, the m2-specific free radical was shown to be located at a tyrosine residue. of the known tyrosine-free radicals of ribonucleotide r ... | 1982 | 6279610 |
| yeast dna topoisomerase ii. an atp-dependent type ii topoisomerase that catalyzes the catenation, decatenation, unknotting, and relaxation of double-stranded dna rings. | an activity from the yeast saccharomyces cerevisiae, initially noted for its catalysis of aggregation of covalently closed double-stranded dna rings in the presence of atp, has been identified as a type ii dna topoisomerase and is designated yeast dna topoisomerase ii. the formation of the dna aggregate, which has been shown to be a network of dna rings that are topologically interlocked, requires the presence of a yeast dna-binding protein in addition to the topoisomerase. in the absence of the ... | 1982 | 6279616 |
| na-k-dependent atpase in red cells and thyroid status. | the relationship between ouabain-sensitive atpase (na-k atpase) activity in erythrocytes and the thyroid status was studied in 36 patients with graves' disease and 58 patients receiving l-thyroxine (t4) replacement therapy. forty normal children served as control. total atpase activity in 4 untreated hypothyroid patients was significantly reduced (11.0 +/- 4.6 vs 17.3 +/- 4.1 micrograms-p/h/mg-protein, p less than 0.01), and na-k atpase was undetectable, both of which were normalized after 4 wee ... | 1982 | 6303767 |
| effects of tsh on camp levels and thyroid hormone release in human thyroid 'autonomous' nodules: relationship with iodothyronine and iodine content in thyroglobulin. | the effect of tsh on the adenylate cyclase-camp system and in vitro iodothyronine release, together with the iodothyronine and iodine content of 19s thyroglobulin, were studied in seven clinically euthyroid patients with autonomous thyroid nodules. basal camp and cgmp content together with phosphodiesterase and protein-kinase activities were normal in nodular, and suppressed in extranodular tissue. tsh-dependent camp accumulation was reduced in nodular tissue, but normal in the suppressed extran ... | 1982 | 6299622 |
| effects of thyroxine on transmembrane resting potentials of skeletal muscle cells in culture. | the effect of thyroxine (t4) was studied on the transmembrane resting potential (em) of rat skeletal muscle cells in culture. in agreement with previous studies, we found the em of developing muscle cells to increase with age from day 3 to day 7 or 8, at which time a plateau is reached. the values of em we obtained (-65 to -75 mv) were 15-20 mv higher than those (-50 to -55 mv) reported by others. treatment of mature myotubes with t4 resulted in a significant increase in resting em. there was on ... | 1982 | 6298437 |
| [comparison of the values of total thyroxine (t4), radiotriiodothyronine binding coefficient (t3cu) and the free thyroxine index (ft4i) in the serum of premature infants in relation to their gestational age]. | 1982 | 6304610 | |
| effect of proofreading and dam-instructed mismatch repair systems on n4-hydroxycytidine-induced mutagenesis. | the role of the proofreading (3' leads to 5' exonuclease) function of t4 dna polymerase and the mismatch repair system of e. coli on n4-hydroxycytidine (oh4cyd) induced mutagenesis was investigated. oh4cyd-induced mutation is strongly suppressed when the proofreading activity increases as a result of the presence of tscb87--antimutator polymerase or elevated temperature (43 degrees c vs 30 degrees c). mutagenic activity of oh4cyd, however, is little, if at all, affected by the presence of the ts ... | 1982 | 6750321 |
| aberrant immunoregulatory control of b lymphocyte function in lepromatous leprosy. | the capacity of peripheral blood mononuclear (pbm) cells from patients with leprosy to generate immunoglobulin-secreting cells in response to pokeweed mitogen (pwm) was evaluated by a reverse haemolytic plaque forming cell (pfc) assay. the pfc responses of pbm cells from patients with lepromatous (lpr) leprosy were significantly higher (p less than 0.01) than those of pbm cells from normal controls and patients with tuberculoid leprosy. co-culture of t lymphocytes from normal donors with pbm cel ... | 1982 | 6751630 |
| [haptoglobins, streptococcus agglutination and streptococcal infections]. | the haptoglobins in man and in mammals react with streptococci bearing the t4-antigen. hp 2-2 and hp 2-1 react with high titres like complete agglutinins while hp 1-1 acts like a blocking antibody. it is surprising to observe the similarity of the behaviour of the haptoglobins and of igg and igm. streptococci carrying hp 1-1 yield a positive coombs-test. the coombs-serum however, is an anti-human-haptoglobin-body from sheep. by means of supplements it has been possible to increase partly conside ... | 1982 | 6752004 |
| fcigg receptor-bearing lymphocytes and monoclonal antibody-defined t cell subsets in atopic dermatitis: effect of treatment with thymopoietin pentapeptide (tp-5). | lymphocyte subpopulations were determined in the blood of patients with atopic dermatitis (ad) before and after treatment with the thymopoietin pentapeptide tp-5. the relative and absolute numbers of lymphocytes bearing suppressor/cytotoxic cell markers (fcigg+e+ and t8+ cells) were significantly decreased in the untreated patients and the t4+/t8+ cell ratio was increased, indicating an imbalance between lymphocyte subpopulations in ad. patients who had been treated for 6 weeks with tp-5 display ... | 1982 | 6752042 |