Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the preparative synthesis of oligodeoxyribonucleotides using rna ligase. | the synthesis of nmol quantities of defined sequences of oligodeoxyribonucleotides using t4 rna ligase has been demonstrated. reacting using from 18 to 200 nmol of substrates in which a single 2'-deoxyribonucleoside 3',5'-bisphosphate was added to an oligodeoxyribonucleotide resulted in yields from 13 to 95%. when two oligodeoxyribonucleotides were similarly joined using rna ligase, the yields ranged from 10 to 50%. although the reactions contained high concentrations of enzyme and were incubate ... | 1982 | 7043396 |
| are dna precursors concentrated at replication sites? | we have asked whether the effective concentrations of deoxyribonucleotide 5'-triphosphates (dntps) at sites of dna replication in vivo might be higher than the concentrations of dntps averaged over the entire cell volume. the approach involved determination of the dependence of dna replication rate upon thymidine triphosphate concentration, both in vivo and in vitro system that closely approximates the intracellular replication apparatus. in t4 phage-infected escherichia coli maximal rates of dn ... | 1982 | 7043458 |
| inhibitory effect of hydroquinone and related agents on multiplicity reactivation and genetic recombination of bacteriophage t4. | the multiplicity reactivation of t4d phage irradiated with uv was inhibited by treatment of infected cells with 0.5 mm hydroquinone (hq). hq also inhibited genetic recombination of t4b rii mutants. the recombination frequency was reduced by treatment of infected cells with hq in a buffer when compared with an untreated control. in addition to hq, the effects of quinone, an oxide form of hq, mercaptoethanol and alpha-tocopherol, antioxidants, were also investigated. quinone and mercaptoethanol in ... | 1982 | 7043791 |
| thyroglobulin degradation by thyroidal proteases: action of thiol endopeptidases in vitro. | we have obtained evidence of thiol endopeptidases in the thyroid which are active in thyroglobulin degradation in vitro. four pepstatin-insensitive endopeptidase fractions were distinguished in extracts of rabbit thyroids by gel filtration on bio-gel a-0.5m. an enzyme from one fraction was obtained in highly purified form and was found to be identical to cathepsin b described in other tissues. endopeptidases in the three remaining fractions were designated as cathepsins 180k, 110k, and 45k, resp ... | 1982 | 7044763 |
| a study of the mechanism of t4 dna polymerase with diastereomeric phosphorothioate analogues of deoxyadenosine triphosphate. | t4 dna polymerase copolymerizes the sp isomers of 2'-deoxyadenosine 5'-o-(1-thiotriphosphate) and 5'-o-(2-thiotriphosphate) with dttp onto a poly(d(a-t) template in the presence of various metal ions. the corresponding rp diastereomers are inactive, independent of the metal ion used. the polymer resulting from the polymerization of the sp diastereomer of 2'-deoxyadenosine 5'-o-(1-thiotriphosphate) and dttp can be degraded by the 5' leads to 3' exonuclease activity of escherichia coli dna polymer ... | 1982 | 7045112 |
| demonstration of pyrimidine dimer-dna glycosylase activity in vivo: bacteriophage t4-infected escherichia coli as a model system. | an approach to the detection of pyrimidine dimer-dna glycosylase activity in living cells is presented. mutants of escherichia coli defective in uvr functions required for incision of uv-irradiated dna were infected with phage t4 denv+ or denv- (defective in the t4 pyrimidine dimer-dna glycosylase activity). in the former case the denv gene product catalyzed the incision of uv-irradiated host dna, facilitating the subsequent excision of thymine-containing pyrimidine dimers. isolation of these di ... | 1982 | 7045391 |
| simultaneous detection of thyroglobulin (tg), thyroxine (t4), and triiodothyronine (t3) in nontoxic thyroid tumors by the immunoperoxidase method. | in 97 nontoxic thyroid tumors, detection of thyroxine(t4) and triiodothyronine(t3) was attempted by the immunoperoxidase method. t4 was demonstrated in 58 tumors (59.8%) and t3 in 76 (78.4%). the feasibility of biosynthesis of t4 and t3 by such tumors was thus established. in 65 of the tumors, we applied the immunostaining method to serial or semiserial sections to study the correlation among the localizations of thyroglobulin(tg), t4, and t3. the localization of t4 agreed relatively well with t ... | 1982 | 7046455 |
| biochemical and clinical effects of fenclofenac in thyrotoxicosis. | administration of the anti-inflammatory drug fenclofenac (600 mg bd) for 28 days to four females with thyrotoxicosis resulted in a rapid decline in thyroid hormone levels. the mean total thyroxine (t4) level of 173 +/- 8.6 (sem) nmol/l before therapy was normalized to 70 +/- 6.4 nmol/l after administration of the drug for 7 days. free t4 levels also decreased significantly but not to within the reference range in all subjects. mean total triiodothyronine (t3) and reverse t3 levels declined from ... | 1982 | 7047000 |
| roles of lipopolysaccharide and outer membrane protein ompc of escherichia coli k-12 in the receptor function for bacteriophage t4. | the roles of lipopolysaccharide and ompc, a major outer membrane protein, in the receptor function for bacteriophage t4 were studied by using escherichia coli k-12 strains having mutations in the ompc gene or in genes controlling different stages of lipopolysaccharide synthesis. the receptor activity for t4 was monitored by (i) t4 sensitivity of intact cells, (ii) phage inactivation activity of cell envelopes, and (iii) phage inactivation activity of specimens reconstituted from purified ompc an ... | 1982 | 7047495 |
| radioimmunoassay of thyroxine (t4), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (reverse t3, rt3), and 3,3'-diiodothyronine (t2). | 1982 | 7048011 | |
| role of the constant uridine in binding of yeast trnaphe anticodon arm to 30s ribosomes. | twenty-two anticodon arm analogues were prepared by joining different tetra, penta, and hexaribonucleotides to a nine nucleotide fragment of yeast trnaphe with t4 rna ligase. the oligomer with the same sequence as the anticodon arm of trnaphe bind poly u programmed 30s ribosomes with affinity similar to intact trnaphe. analogues with an additional nucleotide in the loop bind ribosomes with a weaker affinity whereas analogues with one less nucleotide in the loop do not bind ribosomes at all. reas ... | 1982 | 7048255 |
| [multivariate correspondence analysis of a visually screened eeg. application to a study of psychotropic drugs compared with placebos (author's transl)]. | eighty-seven eeg recordings were obtained from 9 healthy volunteers, medical students who were part of a controlled, double-blind, randomized study. after a first control recording, they received orally, at 1 week interval, either 3 placebos or 3 compounds: yohimbine chlorhydrate (4 mg), propranolol (40 mg), a psychotropic compound in experimentation. the polygraphic recordings were obtained 2 or 6 h after placebos or active compounds. four eeg channels were recorded simultaneously (t4-c4, t3-c3 ... | 1982 | 7048451 |
| [propranolol effect on renin-angiotensin system kinetics during experimental hyperthyroidism (author's transl)]. | the basal levels of angiotensin i (a. i), plasma renin activity (pra), plasma renin substrate (prs) and plasma renin concentration (prc) were estimated in hyperthyroid rats before and after propranolol treatment. serum levels of triiodothyronine (t3), thyroxine (t4), angiotensin i both basal and generated by incubation of plasma samples were measured by radioimmunoassay, while, prc and prs were evaluated by triiodothyronine or thyroxine treatment induced a decrease in prs and an increase in prc ... | 1982 | 7048455 |
| [immunofluorescent observation of t4 in endemic goiter tissue (author's transl)]. | 1982 | 7049621 | |
| relative importance of corticosterone and thyroxine in the postnatal development of sucrase and maltase in rat small intestine. | both corticosterone and t4 have been previously implicated as causal factors in the ontogenic increases in jejunal sucrase and maltase activities during the third week of life in the rat. furthermore, it is known that the administration of exogenous t4 during the developmental period causes significant increases in serum corticosterone concentrations. to determine whether the effects of t4 on sucrase and maltase are secondary to the corticosterone rise, we examined the effect of t4 administratio ... | 1982 | 7049675 |
| characterization of the active site of ribonucleotide reductase of escherichia coli, bacteriophage t4 and mammalian cells by inhibition studies with hydroxyurea analogues. | 1982 | 7049700 | |
| a structurally modified yeast trnaphe with six nucleotides in the anticodon loop lacks significant phenylalanine acceptance. | the deletion of nucleoside 37 from yeast trnaphe was accomplished in a three-step procedure that involved (i) specific depurination of this purine under acidic conditions and removal of the carbohydrate moiety with aniline-2-aminopyridine, (ii) removal of phosphate monoesters from the 5'-half-molecule with alkaline phosphatase, and (iii) resealing of the anticodon loop with t4 rna ligase. the course of the resealing reaction was monitored by polyacrylamide gel electrophoresis and found to be com ... | 1982 | 7050115 |
| urinary immunoprecipitation method for estimation of thyroxine to triiodothyronine conversion in altered thyroid states. | a new method is described for the estimation of t4 to t3 conversion in man and is applied to the study of hyperthyroid and hypothyroid clinical states. the method employs simultaneous iv injection of [125i]t4 and [131i]t3 with isolation of the labeled t3 tracers in 4- to 8-day pooled urine samples by a combination of solvent extraction, desalting, and immunoprecipitation procedures. using [131i]t3 as a recovery standard, the t4 to t3 conversion ratio was found to be 0.470 +/- 0.011 in euthyroid ... | 1982 | 7050140 |
| effect of thyrotoxicosis on ethanol metabolism and on hepatic ethanol oxidation enzymes. | the influence and mechanism of action of t4 treatment on ethanol metabolism was investigated by the determination of ethanol elimination in intact rats and by the estimation of alcohol dehydrogenase and the microsomal ethanol oxidizing system activity in isolated rat liver. the rate of ethanol degradation was significantly decreased to 65% of control values in t4-treated rat liver. similarly blood ethanol disappearance rate was significantly inhibited in thyrotoxic rats as compared to euthyroid ... | 1982 | 7050228 |
| mode of action of colicin s8. | the mode of action of colicin s8 has been studied and compared with that of other colicins. two minutes after the addition of colicin s8 to bacteria a considerable proportion of the colicin is inaccessible to trypsin. treatment of bacteria with colicin s8 renders them more sensitive to lysis by sodium dodecyl sulphate and also inhibits motility. like colicins k and e1, colicin s8 provokes lysis of bacteria superinfected with bacteriophage t4. colicin s8, unlike colicin e2, prevents replication o ... | 1982 | 7050299 |
| thermogenesis in the avian body and the role of the pineal in thermoregulation. | in the absence of the brown adipose tissue, maintenance of body temperature (tb) in birds is generally believed to be by shivering thermogenesis (st), though evidence for nonshivering thermogenesis (nst) is becoming available. in st, glycogen utilization by the anaerobic white muscle fibers forms the main energy process, while the aerobic red fibers appear to be largely involved in nst. winter pigeons maintained on a 12 h photophase (pp) and 12 h scotophase (sp) had higher tb during pp in both w ... | 1982 | 7051038 |
| blood transfusion and kidney transplantation: effect of small doses of blood on kidney graft function and survival. | a controlled clinical trial was started to evaluate whether small doses of blood given pretransplant determine a transfusion effect while reducing the risk of antibody production. for this purpose, 65 consecutive never transfused patients suffering from end-stage renal failure were assigned to one of two groups: the first group was transfused with 1 unit of packed red cells (containing a mean of 2350 x 10(6) leukocytes, 900 x 10(6) mononuclear cells) 3 times at 15-day intervals. the second group ... | 1982 | 7051464 |
| t-cell subsets and langerhans cells in normal and diseased conjunctiva. | to identify the infiltrating mononuclear cells in conjunctival lesions, we stained frozen tissue sections of conjunctiva with a series of monoclonal antibodies against t cells and t-cell subsets with an immunoperoxidase method. in vernal conjunctivitis and ocular pemphigoid, most lymphocytes reacted with the anti-t4 antibodies that define helper/inducer t cells (t4+); a minority reacted with the anti-t8 antibody that defines suppressor/cytotoxic t cells (t8+). however, t8+ cells predominated in ... | 1982 | 7051834 |
| the treatment of advanced carcinoma of the bladder with combination chemotherapy. | thirty patients with advanced t4 transitional cell carcinoma of the bladder were treated in a prospective randomised trial with combination chemotherapy. sixteen patients received cyclophosphamide, doxorubicin (adriamycin) and 5-fluorouracil, whilst 14 patients received methotrexate, doxorubicin and 5-fluorouracil with folinic acid rescue. there were no complete or partial responses to either regime and there was progression in all patients. the 2 regimes were shown to be relatively non-toxic bu ... | 1982 | 7052188 |
| thyroxin-evoked increase of sucrase activity in lower villus in the jejunum of adrenalectomized suckling rats. | intestinal sucrase activity, absent in the suckling rat, is evoked precociously by thyroid hormones. since enterocytes migrate from crypt to villus tip, the question arises: at which level of the villus--crypt columns do the enterocytes respond with an increase of sucrase activity to thyroxin (t4)? suckling rats (11 days old) were injected daily, subcutaneously, with t4 (2 micrograms/g bw/day). they were sacrificed 1, 2, and 3 days later. sucrase activity was determined in homogenates of the ent ... | 1982 | 7186070 |
| sex differences of japanese quails in response to acute cold exposure. changes in thyroid activity and blood glucose level. | sudden exposure of japanese quails to cold (-1 to -3 degrees c) decreases the skin temperature during 0.5 to 3 hrs without any effect on the deep body temperature. the fall in skin temperature was about two times faster in males than in females. this indicates greater resistance to cold of females in comparison with male quails. greater resistance to cold was accompanied by an increase in thyroid activity and earlier decrease in blood glucose level which may suggest that mobilization of these co ... | 1982 | 7186761 |
| marker-dependent recombination in t4 bacteriophage. i. outline of the phenomenon and evidence suggesting a mismatch repair mechanism. | in standard crosses, some riib mutants of t4 phage were found to be susceptible to an extra recombination mechanism to which the other mutants were much less susceptible. the following observations were interpreted as evidence for the mismatch-repair nature of the phenomenon: (1) marker-dependent recombination generates exclusively double exchanges at both sides of the marker. (2) marker-dependent recombination is highly sensitive to dna base sequence at the site of the marker and to that at the ... | 1982 | 7187359 |
| marker-dependent recombination in t4 bacteriophage. ii. the evaluation of mismatch repairabilities in crosses within indicator distances. | the contribution of mismatch repair to genetic recombination in t4 phage has been evaluated by three independent approaches: (1) testing for non-additivity of recombinant frequencies; (2) measurements of double exchange frequencies in three-factor crosses: (3) comparisons of recombination abilities of mutations occupying the same site. quantitative agreement among the results of these approaches suggests that within distances much less than the mean length of hybrid regions, mismatch repair acco ... | 1982 | 7187360 |
| methyl methanesulfonate mutagenesis in bacteriophage t4. | mms induces diverse rii mutations from a wild-type background in bacteriophage t4. about 56% are base pair substitutions, about 30% are frameshift mutations, and the remainder is a miscellaneous set of rapidly reverting or leaky mutants of unknown composition; but deletions were not detected. mms-induced forward mutation is sharply reduced by the mutations px and y, which also reduce ultraviolet, photodynamic and gamma-ray mutagenesis and increase killing by all of these agents. thus, many of th ... | 1982 | 7187361 |
| structure-function relationship in thyroglobulin: amino acid sequence of two different thyroxine-containing peptides from porcine thyroglobulin. | from the cyanogen bromide (cnbr) treatment of porcine thyroglobulin a peptide of mol. wt. 15 000, cnbr-b1, was purified by gel filtration and ion-exchange chromatography. cnbr-b1 contained 50% of the thyroxine (t4) content of the protein. after digestion with trypsin and protease from staphylococcus aureus v-8, thyroxine-containing peptides were purified and analyzed by microsequence analysis using the colored edman's reagent dimethylaminoazobenzeneisothiocyanate . two different sequences harbor ... | 1982 | 7188347 |
| [effect of escherichia coli mutation affecting the rna polymerase sigma factor on phage t4 development]. | the bacterial rna polymerase sigma factor is necessary throughout t4 development. t4 can develop in the e. coli rpod800 mutant cells only at permissive temperature. rna synthesis in t4-infected mutant cells remains temperature-sensitive throughout infection as in uninfected mutant bacteria. this shows that bacterial sigma factor is necessary for all types of rna synthesis in infected e. coli. the data obtained suggest also that active sigma factor is necessary for early, but not for late t4 dna ... | 1982 | 7040940 |
| sealing of gaps in duplex dna by t4 dna ligase. | single-strand gaps in dna molecules were found to be a substrate for t4 dna ligase. sealing of the gaps was optimal at the same conditions as ligation of blunt-ended dna molecules. spermidine at a concentration of 2 mm stimulated the ligation of gaps, as well as the joining of dna molecules with cohesive and blunt ends. in addition, spermidine reduced the optimal atp concentration. the ligation of single-stranded gaps was a slow process, reaching a plateau after several hours at 25 degrees c. ap ... | 1982 | 7041091 |
| two alternative mechanisms for initiation of dna replication forks in bacteriophage t4: priming by rna polymerase and by recombination. | we show that bacteriophage t4 has two alternative mechanisms to initiate dna replication; one dependent on escherichia coli rna polymerase (rna nucleotidyltransferase, ec 2.7.7.6), and one dependent on general recombination. continued dna synthesis under recombination-defective conditions was sensitive to rifampin, an inhibitor of rna polymerase. on the other hand, dna synthesis accelerated in spite of the present of rifampin if recombination occurred. | 1982 | 7041114 |
| familial thyroid hormone resistance. | three phenotypically normal family members were discovered to have elevated thyroid function (t4, free t4, t3, 123i uptake), but were clinically euthyroid. further evaluation of pituitary and peripheral indices of thyroid hormone action was consistent with the diagnosis of peripheral resistance to thyroid hormone. basal metabolic rate, serum cholesterol, pulse wave arrival time (qkd), and serum sex hormone binding globulin levels were all normal. serum tsh was inappropriately elevated for the de ... | 1982 | 7200565 |
| diurnal variations of plasma thyrotropin, thyroxine, and triiodothyronine in female rats are phase shifted after inversion of the photoperiod. | the present study examined plasma tsh, t4, and t3 concentrations in rats throughout the day to determine if diurnal variations of these hormones occurred. female rats on a 12-h light, 12-h dark cycle (onset of light, 0730 h) were sampled by cardiac puncture at 2-h intervals throughout the day on 2 days, 1 week apart. significant diurnal variations of plasma tsh, t4, and t3 were detected (p less than 0.01), peak tsh concentrations occurred soon after the onset of light, whereas t4 and t3 concentr ... | 1982 | 7201383 |
| ultrastructure of normal human blood t lymphocyte subsets isolated by cell sorting using monoclonal antibodies. a stereological analysis. | in continuation of a previous study, the quantitative fine structural characteristics of the average normal human pan-t lymphocyte and its subsets in the peripheral blood has been established using stereological methods. t cell subpopulations were isolated and identified by means of monoclonal t3, t4 and t8 antibodies and a fluorescence-activated cell sorting machine. comparative studies on ultrastructural morphology were made between the e-rosetting lymphocyte and the t3-positive cell, both mar ... | 1982 | 6134377 |
| role of n-acetyltransferase phenotypes in bladder carcinogenesis: a pharmacogenetic epidemiological approach to bladder cancer. | a large excess of patients with bladder cancer who have previously been exposed to n-substituted aryl compounds as a result of the production of dyestuff intermediates have the slow phenotype of the enzyme n-acetyltransferase. among bladder-cancer patients in general, those presenting with t3 or t4 disease or carcinoma-in-situ also show an excess of the slower subtypes. either n-substituted aryl compounds more frequently produce tumours with this invasive potential if linked with slow acetylatio ... | 1982 | 6126711 |
| hyperthyroxinaemia due to decreased peripheral triiodothyronine production. | two patients, a boy of 8 and a women of 60 years of age, had higher than normal levels of serum total thyroxine (t4), free t4, (ft4), ft4 index, and reverse triiodothyronine, but normal serum triiodothyronine (t3) levels. the pituitary-thyroid axis could be normally stimulated by thyrotropin-releasing hormone, suggesting euthyroidism at the pituitary level. high levels of serum t4-binding globulin decreased during t3 treatment in the boy. studies show that in these patients a raised serum ft4 is ... | 1982 | 6126713 |
| attenuation by hypocalcemia of pulsatile growth hormone secretion in conscious male rats. | the effect of hypocalcemia following parathyroidectomy (ptx) on growth hormone (gh) secretion was investigated in unrestrained, unanesthetized male rats bearing chronically implanted indwelling cannulae. during a 6-hour period, starting at about 10 a.m., control rats with a serum calcium (ca) value of 8.11 +/- 0.38 mg/dl (mean +/- sem) 2 weeks after sham-operation showed secretory bursts of gh similar to those observed in conscious intact rats. under hypocalcemia of 4.88 +/- 0.32 mg/dl 2 weeks a ... | 1982 | 6130487 |
| cloning of a gene responsible for the biosynthesis of glutathione in escherichia coli b. | a gene (gshi) responsible for gamma-glutamylcysteine synthetase (gsh-i) activity was cloned to construct an escherichia coli b strain having high glutathione synthesizing activity. for this purpose, two e. coli b mutants (strains c912 and rc912) were used. c912 was deficient in gsh-i activity. rc912, a revertant of c912, had a gsh-i activity that was desensitized to feedback inhibition of reduced glutathione. to clone gshi, chromosomal dnas of rc912 and plasmid vector pbr322 were digested with v ... | 1982 | 6130744 |
| beta-blockers and thoracic epidural analgesia. cardioprotective and synergistic effects. | seven groups of patients with and without hypertension or with ischaemic heart disease, treated with different beta blockers were investigated to study the circulatory effects of neurolept anaesthesia alone or combined with thoracic epidural analgesia from t4 to t12/l2 during abdominal surgery. the combination of thoracic epidural analgesia and neurolept anaesthesia in hypertensive subjects treated with non-cardioselective beta blockers induced slightly lower blood pressure than measured in simi ... | 1982 | 6152883 |
| isolation, properties and nucleolytic degradation of chromatin from escherichia coli. | a new procedure has been developed for the isolation of the chromosome complex, termed chromatin, from escherichia coli. the bacteria were subjected to low ionic strength and t4 lysozyme, followed by detergent treatment analogous to that employed for the isolation of eukaryotic chromosomes. the chromatin was an insoluble viscous material which contained approximately equal amounts of dna and rna. the protein content of the chromatin was almost three times greater than the nucleic acid content. e ... | 1982 | 6190986 |
| postnatal thyroid function in low birth weight infants: a cross-sectional assessment of free thyroxine and thyroid hormone binding globulin. | to investigate the significance of low serum thyroxine in premature infants, serum ft4, t4, tsh and tbg were measured in 7 infants with bw less than 1000 g, 8 infants with bw 1001 to 1350 g, 9 infants with bw 1351 to 2499 g, and 11 full-term infants. ft4 concentrations were lower in the lbw infants than in the ft infants. percent ft4 values in the infants with bw less than 1000 g were the highest in the groups studied, so that ft4 concentrations in those infants did not fall proportionally with ... | 1982 | 6189716 |
| restricted antigenicity of thyroxyls in human thyroglobulin. | interactions between two human iodothyronine-binding autoantisera and three preparations of human thyroglobulin (tg) were not proportional to the latter's thyroxyl residue content. probably only one of the several thyroxyl-containing sites in tg reacted with the immunoglobulins from both antisera. in the case of one of the antisera, which was thyroxine (t4)-specific, the thyroxyl residue was the immunodominant feature of the antigenic site. the other antiserum, which had a specificity for 3,5,3' ... | 1982 | 6187336 |
| a comparison of free t4 and the ratio of total t4 to t4-binding globulin in serum through pregnancy. | serum free t4 concentrations have been variously reported as either constant or falling in pregnancy. in this study, 122 serum samples from apparently normal pregnancies were used to derive euthyroid ranges throughout pregnancy for t4 and free t4 (measured by the amerlex kits) and for t4-binding globulin (tbg). the mean free t4 concentration fell with increasing gestational age, the range of values in the third trimester being narrower than the non-pregnant normal range. evidence is reviewed whi ... | 1982 | 6187501 |
| effect of acetylated and deacetylated 2-aminofluorene adducts on in vitro dna synthesis. | we have constructed primed phi x174 dna templates containing either acetylated or deacetylated aminofluorene adducts at the c-8 position of guanine. t4 dna polymerase terminates synthesis one nucleotide before the acetylated adducts but incorporates an additional nucleotide opposite the deacetylated guanylaminofluorene. these observations can be explained by the known preferred conformations of the acetylated and deacetylated guanosinylaminofluorene nucleosides--the former favoring the syn confo ... | 1982 | 6185946 |
| radioimmunoassayable prostate-specific acid phosphatase in peripheral and bone marrow sera compared in diagnosis of prostatic cancer patients. | measurements of human prostate-specific acid phosphatase by radioimmunoassay in peripheral and bone marrow sera were compared. we studied 20 patients with benign prostatic hyperplasia, 27 with untreated prostatic cancer without bone metastases and 11 with metastases, in addition to 7 with cancer treated by hormonal therapy. the prostate-specific acid phosphatase concentrations in peripheral and bone marrow serum samples were equal and did not exceed the upper limit of our health-associated refer ... | 1982 | 6183458 |
| effect of thyroxine on molecular properties of native and dansylated thyroxine-binding globulin. | the effect of thyroxine (t4) on the stability of thyroxine-binding globulin (tbg) was investigated by examining intrinsic (tryptophan) or extrinsic (dns) fluorescence as structural probes to demonstrate the presence of an induced conformation change. a thermal transition was observed at 55 degrees c for tbg by trp fluorescence, which is evidence for heat denaturation, whereas no transition was observed in the same temperature range for tbg-t4. acidification of dns-tbg at ph 4.4 produced a decrea ... | 1982 | 6184213 |
| characterization of human t suppressor-inducer, -precursor and -effector lymphocytes in the antigen-specific plaque-forming cell response. | suppression of an antigen-specific plaque-forming cell response of human blood lymphocytes can be effected by t mu+ cells that have been primed previously by antigen in vitro for 6 days. while lacking the capacity to suppress the plaque-forming response directly, these primed t mu+ suppressor-inducer cells stimulate a subpopulation of unprimed t mu gamma- cells to differentiate to t gamma + suppressor-effector cells. the t mu+, t gamma+ and t mu gamma- subsets have been shown to be heterogeneous ... | 1982 | 6184239 |
| preoperative chemo-radiotherapy in advanced head and neck cancer. | in a prospective evaluation, two preoperative courses of methotrexate, bleomycin, and cisplatin combined with 2,000 rad/10 fractions ("chemo-radiotherapy") yielded 78% responses rates in previously untreated advanced head and neck cancer. similarly staged patients receiving preoperative irradiation of 5,000 rad alone had a 67% response rate. treatment-related mortality in the chemo-radiotherapy patients was equal to that seen with standard combinations of surgery and preoperative or postoperativ ... | 1982 | 6184328 |
| the in vitro transcription-translation of dna and rna templates by extracts of rhodopseudomonas sphaeroides. optimization and comparison of template specificity with escherichia coli extracts and in vivo synthesis. | a dna-directed coupled transcription-translation system has been developed in cell-free extracts from the facultative phototroph, rhodopseudomonas sphaeroides. the in vitro protein-synthesizing system was active when prepared from either chemoheterotrophically or photoheterotrophically grown cells. optimal activity was dependent upon: use of extracts prepared freshly from early exponential phase cells, the method of cell breakage, and the length of preincubation of the extract (s-30), as well as ... | 1982 | 6184370 |
| vitamin a and beta-carotene content of seminal fluid and sperm of normospermic and oligozoospermic men. | beta-carotene and vitamin a were estimated in 125 specimens of human seminal fluid and spermatozoa. in 72 of the specimens triiodothyronine, tetraiodothyronine, and t4-binding globulin were also determined. vitamin a in seminal fluid increased from 10.4+/-l.8 microgram (se) in severe oligozoospermia up to 19.1+/-2.5 microgram/100ml in specimens with high sperm densities. in spermatozoa, however, there was a trend for a decrease in vitamin content from 2.1+/-0.8 to 0.2 +/-0.07 microgram/108. in t ... | 1982 | 6175287 |
| a comparison of methods for assessing thyroid function in nonthyroidal illness. | we studied various tests of thyroid function in sick patients with nonthyroidal illness (nti) in order to determine the utility of each test for differentiating these patience from a group with hypothyroidism. we evaluated each test in 22 healthy volunteers who served as controls, 20 patients with hypothyroidism, 14 patients admitted to medical intensive care unit whose serum t4 was less than 5 micrograms/dl, 13 patients with chronic liver disease, 32 patients on chronic hemodialysis for renal f ... | 1982 | 6172442 |
| vitamin b-12 (cobalamin) deficiency: a heretofore undescribed control mechanism for plasma corticosteroid-binding globulin concentration in man. | six of eight patients with addisonian pernicious anemia were found to have diminished corticosteroid-binding globulin (cbg) concentrations, which returned within 2 weeks to normal after the im administration of cobalamin. t4-binding globulin was found to be completely normal before and after cobalamin replacement. other estrogen-responsive proteins, i.e. ceruloplasmin, alpha 1-trypsin inhibitor, haptoglobin, transferrin, and alpha 2-macroglobulin, also did not follow cbg concentration in a paral ... | 1982 | 6172443 |
| concanavalin a supernatant recruits antigen-insensitive igg memory b lymphocyte precursors into an antigen-sensitive precursor pool. | spleen cells from mice primed to trinitrophenyl-keyhole limpet hemocyanin (tnp-klh) generate igg anti-tnp memory responses when stimulated in vitro with either thymus-dependent (td) or thymus-independent (ti) forms of the hapten. when supernatants from con a-stimulated spleen cells (con a sup) were added to such secondary cultures the ti responses to dnp-dextran or tnp-t4 were augmented; the td response to tnp-klh was suppressed. passage over sephadex and addition of alpha-methyl-d-mannoside did ... | 1982 | 6172504 |
| effects of thyroxine binding on the stability, conformation, and fluorescence properties of thyroxine-binding globulin. | the effects of thyroxine (t4) on several molecular properties of human thyroxine-binding globulin (tbg) have been evaluated. changes in the sedimentation constant and relaxation time indicate that tbg becomes more symmetric and compact when t4 is bound. this modification in structure is associated with an increase in the stability of tbg to denaturation by either acid or guanidinium chloride. t4 binding also produces changes in the emission and excitation spectra of tbg, reflecting different env ... | 1982 | 6174149 |
| characterization of high- and low-metastatic clones derived from a methylcholanthrene-induced murine fibrosarcoma. | a methylcholanthrene-induced fibrosarcoma (3am) and several of its clones were evaluated for pulmonary metastasis, growth rate, and chromosome composition. heterogeneity was observed in the three parameters, and no correlation was found between growth rate and metastatic potential. furthermore, three clones (10, 34, and 27) were identified with distinctive, high or low, metastatic potential and marker chromosomes. the marker chromosomes characteristic for each clone were identified in the early ... | 1982 | 6174210 |
| comparative effects of thyroxine (t4) and triiodothyronine on t4-binding globulin metabolism in rhesus monkeys. | 1982 | 6174320 | |
| bleomycin, cyclophosphamide and radiotherapy in regionally advanced epidermoid carcinoma of the head and neck. | we have treated 24 patients with squamous carcinoma of the head and neck and advanced regional (n2-3) disease. the regimen consisted of 3 cycles, each of 28 days. cyclophosphamide (1 gm/m2 i.v.) was given on day 1, bleomycin (15 u i.m.) on days 2, 4, 9 and 11, and ionizing radiation (60co, 180 rad/fraction) days 1-5, and 8-12. no therapy was given on days 13-28. after three cycles of therapy, 13 patients had a complete response; following further therapy (surgery, interstitial or external beam r ... | 1982 | 6174492 |
| [free thyroxine estimation by the radioimmunoassay for t4 and tbg (author's transl)]. | 1982 | 6176375 | |
| nuclear triiodothyronine binding in mononuclear leukocytes in normal subjects and obese patients before and after fasting. | we evaluated the maximum binding capacity (mbc) and dissociation constant (kd) of nuclear t3 receptors (nt3r) in mononuclear cells in normal and obese subjects before and after fasting. mononuclear cells were isolated by isopycnic centrifugation of 50 ml heparinized blood underlayered with ficoll-paque. from 1-5 x 10(6) cells (12-60 microgram dna) were incubated for 2 h at 37 c in 0.5 ml ham's f-10 medium with 0.5% bovine serum albumin, 25 mm hepes buffer (ph 7.4), and six t3 concentrations, eac ... | 1982 | 6176590 |
| serum free thyroxine and triiodothyronine concentrations in subacute thyroiditis. | serum absolute free t4 (aft4) and t3 (aft3) concentrations were studied in 10 patients with subacute thyroiditis whose total serum t4 levels were elevated, and values were compared with those obtained in 10 patients with untreated graves' disease, whose total serum t4 concentrations were elevated to the same level as in the former group. the mean (+/- sd) basal metabolic rate in patients with subacute thyroiditis was 16 +/- 14.8%, significantly (p less than 0.001) lower than that in patients wit ... | 1982 | 6176591 |
| [ok t-positivity in stimulated t-lymphocytes]. | t-lymphocytes express different antigenic determinants which can be recognized using specific anti-t monoclonal antibodies. ok t3 (ortho diagnostics, raritan, n.j.) detects 95% circulating t-lymphocytes, while ok t4 reacts with helper/inducer t-lymphocytes and ok t8 with suppressor/cytotoxic t-lymphocytes. in normal peripheral blood the proportion of mononuclear cells (after "lymphoprep' separation) positive with the various anti-t monoclonal antibodies is, according to our standards, as follows ... | 1982 | 6177325 |
| [free thyroxine estimation by the radioimmunassay for t4 and tbg (author's transl)]. | 1982 | 6177450 | |
| [reciprocal changes in serum tbg and t4 in hyperthyroidism treated with ptu and iodide (author's transl)]. | 1982 | 6177452 | |
| plasmid pr386 renders escherichia coli cells restrictive to the growth of bacteriophage t4 unf mutants. | the introduction of the f1 incompatibility group plasmid pr386 tc into several common laboratory strains of escherichia coli rendered them restrictive to the growth of bacteriophage t4 unf mutants, which are defective in unfolding the host genome. the growth inhibition was temperature dependent. the single mutant unf39 x 5 exhibited an efficiency of plating of less than 10(-8) at 27 degrees c. however, at 37 degrees c, complete growth inhibition occurred only when host dna degradation was also a ... | 1982 | 6177866 |
| effect of prazosin on blood lipids and on thyroid function in hypertensive patients. | nineteen patients with essential hypertension (eh) were studied as outpatients. after administration of chlorthalidone, 50 mg/day for 4 weeks, prazosin 1-4 mg/day (1.82 +/- 0.33 mg/day) was added for a period of 12 weeks. prazosin lowered supine blood pressure from 149.7 +/- 2.85/102.0 +/- 2.75 mm hg to 128.2 +/- 3.0/86.1 +/- 1.04 mm hg (p less than 0.001). prazosin did not alter heart rate significantly. prazosin increased the thyroid stimulating hormone (tsh) from 3.63 +/- 0.33 microunits/ml t ... | 1982 | 6177961 |
| i region-restricted t cell line stimulated with hapten-treated syngeneic cells: selection of clones with reactivity for both allogeneic ia determinants and self-i-a plus hapten. | the apparent switch in proliferative t cell reactivity which occurred in a b10 t cell line (b10(t4)) repeatedly stimulated with trinitrobenzene sulfonic acid (hapten)-treated syngeneic cells is described. this functionally heterogenous t cell population, apparently depleted of alloreactivity and strictly dependent on cells expressing syngeneic i-a and the hapten for its propagation, lost, after its 10th restimulation, the previously detected t cell functions, and expressed a new pattern of proli ... | 1982 | 6178594 |
| decrease of immunoglobulin g-fc receptor-bearing t lymphocytes in graves' disease. | peripheral immunoglobulin g (igg)-fc receptor-bearing t lymphocytes (t gamma cells) in patients with autoimmune thyroid diseases and destruction-induced thyrotoxicosis were assayed by measuring erythrocyte antibody-rosette formation using a microplate technique. in untreated patients with graves' disease, the percentage of t gamma lymphocytes (mean +/- sd, 8.9 +/- 3.9%; n = 15) was significantly lower (p less than 0.001) than that in normal controls (21.7 +/- 9.1%; n = 35). a similar decrease wa ... | 1982 | 6178751 |
| detection of a cellular polypeptide associated with adenovirus-coded va rna using in vitro labeling of proteins cross-linked to rna. | ultraviolet light induced rna-protein cross-linking for identification of polypeptides interacting with rna in intact cells (wagenmakers et al. 1980), is limited by the intensity of the label in the proteins or in residual nucleotides remaining attached to the proteins after rnase treatment of the rna-protein complexes. here we report a method, where th cross-linked rna-protein complexes are treated with rnase t1 and the t1-oligonucleotides covalently linked to the proteins are labeled in the 5' ... | 1982 | 6179041 |
| phenotype of cells involved in mycosis fungoides and sézary syndrome (blood and skin lesions): immunomorphological study with monoclonal antibodies. | the phenotypic expression of immunocompetent cells involved in lesions of cutaneous t-cell lymphoma (mycosis fungoides and sézary syndrome) was studied by using monoclonal antibodies specific for t-cell subsets and hla-dr antigens (okt3, t4, t6, t8) (bl 6, bl 5, bl 2). in sézary syndrome, the phenotype of sézary cell is homogeneous (t3+, t4+, t8-, t6-, bl2-) and corresponds to the phenotype expressed by the normal helper t-cell population. at the ultrastructural level, the discontinuous distribu ... | 1982 | 6179357 |
| loss of thymine dimers from mammalian cell dna. the kinetics for antibody-binding sites are not the same as that for t4 endonuclease v sites. | antiserum specific for thymine-containing dimers was used to assay dna isolated from ultraviolet-irradiated cells following different repair periods. a 50% loss in antibody-binding sites was evident 1 h post-irradiation, and within 4 h 80% of the sites were removed. this result contrasts with data obtained with dimer-specific t4 endonuclease v and does not appear to be due to masking of the dimers by repair enzymes. t4 endonuclease v treatment of ultraviolet-irradiated dna at 0 degree c resulted ... | 1982 | 6179541 |
| a cleavage site of ribonuclease f. a putative processing endoribonuclease from escherichia coli. | a new endoribonuclease activity, rnase f, was partially purified from escherichia coli cells. this activity can specifically cleave a precursor rna molecule (of species 1) isolated from t4-infected cells [n. watson & d. apirion (1981) biochem. biophys. res. commun. 103, 543-551]. the cleavage results in products which are very similar to rna molecules found in the cell, generating a 3'-phosphate and a 5'-hydroxyl groups. the cleavage takes place between a cytosine and an adenine moiety, within a ... | 1982 | 6179777 |
| thymine inhibits suppression by an escherichia coli nonsense and frameshift suppressor. | a mutant strain of escherichia coli suppressed a frameshift and some uag, uaa, and uga mutants of bacteriophage t4 at 37 degrees c but not at 31 degrees c. this suppression was inhibited by the addition of thymine or thymidine to the medium used to test phage growth. furthermore, the suppressor strain required thymine or thymidine for growth on minimal medium at 43 degrees c and if this auxotrophy was removed by reversion or recombination the strain no longer suppressed. these results suggest a ... | 1982 | 6180281 |
| laboratory tests for evaluating thyroid therapy. | appropriate dosage of levothyroxine for the treatment of hypothyroidism is assessed by determining the serum thyroxine (t4) concentration in secondary and tertiary types. in primary hypothyroidism, the optimal thyroid replacement is that which induces a normal thyroid-stimulating hormone level and a normal tsh response to administration of thyrotropin-releasing hormone. hypothyroidism often occurs in the management of hyperthyroidism. serial serum tsh measurements help in the early detection of ... | 1982 | 6180620 |
| [effect of the antitumor antibiotic bleomycin on dna polymerase activity]. | treatment with bleomycin activates considerably a repair synthesis of dna in rat liver chromatin in vitro and can cause loosening of the nucleoprotein complex, which facilitates the accessibility or repair enzymes for lesions in chromatin dna. the bleomycin action on dna-template increases severalfold the rate of synthesis catalyzed by dna polymerase beta inhibits the activity of dna polymerase i from escherichia coli and suppresses severalfold the activity of dna polymerase alpha and dna polyme ... | 1982 | 6180778 |
| alloantigens expressed on a subset of unstimulated human t lymphocytes that generate suppressor function. | human alloantisera were tested for antibodies reacting with t-cell subpopulations. t-cell subsets were separated using the monoclonal antibodies okt4 and okt8. five sera reacting with the t4-t8+ subset and two sera reacting with t4+t8- lymphocytes were identified. serum z. g. reacted with t4-t8+ cells from 8 of a panel of 19 donors. t cells treated with z. g. serum and rabbit complement lost the capacity to generate suppressor cells but showed no decrease in the development of cytotoxic effector ... | 1982 | 6180983 |
| elevation of serum angiotensin-converting enzyme activity in patients with hyperthyroidism. | the activity of serum angiotensin-converting enzyme (s-ace) was determined spectrophotometrically in 45 patients with hyperthyroidism (30 untreated and 15 treated and euthyroid patients), 14 patients with hypothyroidism, and 135 normotensive healthy subjects. s-ace was significantly higher in the patients with untreated hyperthyroidism (51.6 +/- 1.9 less than se greater than nmol.min/ml) than in the healthy controls (28.6 +/- 0.6 nmol.min/ml; p less than 0.001). on the other hand, s-ace was foun ... | 1982 | 6181086 |
| interstimulus interval dependence of the auditory vertex response and its magnetic counterpart: implications for their neural generation. | auditory vertex responses elicited by short tone bursts were compared with their magnetic counter parts. special attention was paid to the behaviour of the n100 deflection of the response. electrical responses were recorded from scalp locations fp2, fz, cz, pz, c4 and t4 and the magnetic responses half way between p4 and t6, at a point where the response has one of its amplitude extrema. different isis (from 1 to 16 sec) were applied in order to differentiate specific and nonspecific evoked pote ... | 1982 | 6181979 |
| effect of thyroxine on protein and nucleic acid contents of different parts of brain of singi fish (heteropneustes fossilis bloch). | effects of three consecutive days injections of thyroxine (t4) of different doses (1, 2 and 4 micrograms/g of body weight) on different parts of the brain of singi fish were studied. t4 enhanced the cranio-somatic index and the weight of different substructures of brain, viz., cerebrum, cerebellum midbrain and medulla oblongata, of singi fish at 25 degrees c. compared to the control, the protein content of cerebrum increased with all doses of t4 (1, 2 and 4 micrograms/g) and the rna content of t ... | 1982 | 6181986 |
| interaction between thyroxine and tricyclic antidepressant drugs on cardiac neurotransmitter receptors. | the effect of chronic administration of desmethylimipramine (dmi) and iprindole on cardiac neurotransmitter receptors was studied. in addition, the influence of these drugs on the action of thyroxine (t4) on myocardial receptors was investigated. administration of iprindole (10 mg/kg i.p., twice daily for 11 days) markedly increased the number of beta- adrenergic receptors in the heart and significantly reduced the affinity of these receptors for (-)[3h]dihydroalprenolol ([3h]dha). injection of ... | 1982 | 6182419 |
| interactions between dna polymerase and aminofluorene adducts that affect the recognition and possibly the mutagenicity of the lesions. | adducts of 2-aminofluorene on the c-8 position of guanine block dna synthesis and lead to mutation. n-acetylated adducts adopt the syn conformation such that in dna the guanine is displaced from the helix by the fluorene ring while unacetylated adducts prefer the anti conformation allowing normal base pairing of the guanine with cytosine. in vitro synthesis by both e. coli dna polymerase i and t4 dna polymerase terminates predominantly one nucleotide before acetylated adducts but has an increase ... | 1982 | 6182925 |
| the prevalence of thyroid-stimulating antibodies in goitrous disease assessed by cytochemical section bioassay. | thyroid-stimulating antibodies (tsab) were detectable using a highly sensitive cytochemical section bioassay in plasma from all 56 hyperthyroid patients studied, including those who had either diffuse hyperplasia or nodular goiters. the maximum dilution at which tsab was detectable ranged from 10(-2) - 10(-6). six of these patients had scintigraphic evidence of single functioning nodules, and, surprisingly, tsab was present in all. of 27 patients who had nontoxic goiter, 14 (52%) had positive ti ... | 1982 | 6119323 |
| adult t-cell lymphoma-leukaemia in blacks from the west indies. | six black patients (five born in the west indies and one in guyana), aged 21-55 years, had adult t-cell lymphoma-leukaemia diagnosed in the u.k. this disorder is rare in europe and the u.s.a., but is more common in japan. five patients had severe hypercalcaemia which correlated with disease activity, although osteolytic lesions were found in only one. other clinical features were lymphadenopathy and a high white blood-cell count (range 27-67 x 10(9)/l) with a predominance of pleomorphic lymphoid ... | 1982 | 6121963 |
| immunoreactive somatostatin and beta-endorphin content in the brain of mature rats after neonatal exposure to propylthiouracil. | the contents of immunoreactive somatostatin (ir-srif) and beta-endorphin (ir-beta-ep) in 12 brain regions were examined in rats exposed neonatally to propylthiouracil (ptu) through the mother's milk. since the dose of ptu used in this study is lower than the usual dose employed to induce hypothyroidism, a milder form of neonatal hypothyroidism resulted. this conclusion is supported by the only mild subnormal growth of rats to adulthood and serum t4 and t3 concentrations in the normal range. adul ... | 1982 | 6122558 |
| fetal and neonatal hazards of maternal hydration with 5% dextrose before caesarean section. | in a prospective randomised study, 47 fasting women undergoing elective caesarean section received one of the following three glucose regimens for prevention of hypotension before administration of epidural bupivacaine to produce anaesthesia from the t4 sensory level: (i) 150 ml of 5% dextrose in water (d5w) and 1000 ml normal saline (7.5 g dextrose); (ii) 150 ml of d5w, 350 ml of 5% dextrose in normal saline (d5ns), and 650 ml of normal saline (25 g dextrose); (iii) 150 ml d5w and 1000 ml d5ns ... | 1982 | 6122939 |
| inhibitor studies of phage t4 wild-type and mutant dna polymerases. ii. differential inhibition by pyridoxal 5'-phosphate. | the sensitivities to pyridoxal 5'-phosphate of phage t4 wild-type and two ts mutant dna polymerases, l98 (mutator) and cb 121 (antimutator), were studied. the wild-type and the mutator enzyme we inhibited to an equal extent, while the antimutator enzyme was six times more sensitive. the mode of inhibition was competitive with the deoxynucleoside triphosphate substrates. the cb121 dna polymerase had a three times lower affinity to its substrates but a twofold affinity to pyridoxal 5'-phosphate. t ... | 1982 | 6124109 |
| chronic exposure to short photoperiod inhibits free thyroxine index and plasma levels of tsh, t4, triiodothyronine (t3) and cholesterol in female syrian hamsters. | 1. chronic exposure of female syrian hamsters (mesocricetus auratus) for 9 weeks to a short photoperiod (10l:14d) depressed the pituitary-thyroid axis as indicated by a drop in circulating titers of thyroid stimulating hormone (tsh), thyroxine (t4), triiodothyronine (t3) and the free thyroxine index (ft4i) compared to animals maintained under long photoperiodic conditions (14l:10d). 2. short day treatment also reduced plasma cholesterol levels. 3. neither plasma triglycerides, glucose nor growth ... | 1982 | 6124346 |
| somatostatin: a paracrine contribution to hypothyroidism in hashimoto's thyroiditis. | thyroid tissue from 18 consecutive cases of hashimoto's thyroiditis treated surgically were stained immunohistochemically for neurone specific enolase (nse), somatostatin, calcitonin and thyroglobulin. cells staining for nse and somatostatin were present in 14 cases. in four cases large numbers of cells including oxyphil cells stained for nse. consecutive sections showed an identical staining pattern of these cells for somatostatin. sections stained for calcitonin showed few or no positively sta ... | 1982 | 6124555 |
| dna synthesis at a fork in the presence of dna helicases. | in a mixture of escherichia coli dna polymerase iii holoenzyme, single-strand-binding protein, artificially forked lambda bacteriophage dna with primer annealed to the leading side of the fork, dntps and atp, dna synthesis is enhanced by helicase ii, less so by helicases, i, iii or rep protein of e. coli or t4 phage helicase. the effect of helicase ii depends on atp, it is enhanced by helicase iii, and it is not observed using dna polymerase i or t4 dna polymerase. in the absence of dntps helica ... | 1982 | 6125389 |
| bacteriophage t4 late promoters: mapping 5' ends of t4 gene 23 mrnas. | more than thirty 5' ends of rna molecules which transverse the bacteriophage t4 late gene, gene 23, have been mapped by nuclease protection and reverse transcription, to a 1.7 kilobase pair (kbp) region upstream of gene 23. most of these 5' ends arise from rna processing and degradation. two sites at which rna synthesis is initiated have been identified by guanylylation of diphosphate- or triphosphate-terminated rna with vaccinia virion "capping" enzyme and are situated approximately 0.1 and 0.9 ... | 1982 | 6201352 |
| alterations of t-cell subsets in primary biliary cirrhosis. | to determine whether abnormalities of immunoregulatory t cells are associated with primary biliary cirrhosis, we characterized peripheral blood mononuclear cells in 16 patients with primary biliary cirrhosis and compared them with 30 normal controls. for this analysis we used monoclonal antibodies to the surface antigens on helper/inducer (t4+) and suppressor (t8+) t cell subsets and to a common t cell antigen (t3+). in contrast to normal persons, patients with primary biliary cirrhosis had redu ... | 1982 | 6210473 |
| influence of thyroid hormone administration on myosin atpase activity and myosin isoenzyme distribution in the heart of diabetic rats. | previous studies have shown that diabetes mellitus leads in rats to a 45% decrease in cardiac ca++ activated myosin atpase, a change in myosin isoenzyme distribution and a lowering of plasma t4 and t3 levels. hypothyroidism causes similar changes in myosin atpase and myosin isoenzyme distribution. we determined if thyroid hormone administration in physiological replacement dose (0.3 microgram t3/100 g bw) or pharmacological doses (3 micrograms t3/100 g bw and 10 micrograms t4/100 g bw) can norma ... | 1982 | 6210824 |
| the relationship between human t-lymphocyte subsets defined by monoclonal antibodies and by avidity differences to sheep erythrocytes. | by e-rosette sedimentation procedures and fluorescence activated cell sorting of monoclonal antibody-coated t lymphocytes, we have investigated the relationship between the human t lymphocyte subsets defined by the monoclonal antibodies against the t4 and t8 antigens and those defined by avidity differences to sheep erythrocytes. through a correlation was demonstrated between the t4+ (inducer) cells and the high avidity ("active") t cells and between the t8+ (suppressor) cells and low avidity t ... | 1982 | 6211030 |
| characterization of immunoregulatory t lymphocytes during ageing by monoclonal antibodies. | monoclonal antibodies of the okt series were used to identify t lymphocytes (okt3+) and their inducer (okt4+) and suppressor-cytotoxic (okt8+) subsets in the peripheral blood mononuclear cells (pbmc) of 32 healthy old-aged people more than 70 years old (16 men and 16 women) compared to 47 adults (29 men, 18 women) less than 40 years old. the absolute lymphocyte count in the peripheral blood was not significantly influenced by age or sex. both the proportions and the absolute numbers of t3+ and t ... | 1982 | 6211314 |
| the regulation of ig synthesis after marrow transplantation. iv. t4 and t8 subset function in patients with chronic graft-vs-host disease. | this study compares pokeweed mitogen-activated immunoglobulin synthesis functions of t, t4 t8, and b cells from 13 marrow graft recipients with chronic graft-vs-host disease (gvhd), 10 long-term healthy marrow graft recipients, and 20 normal individuals. t cells expressed helper function (greater than 20% of the control) in eight of 10 long-term healthy patients and in only four of 13 patients with chronic gvhd. t4 cells expressed helper activity in all 10 long-term healthy patients, whereas t4 ... | 1982 | 6211479 |
| childhood multiple sclerosis: clinical features and demonstration of changes in t cell subsets with disease activity. | using monoclonal antibodies that identify t cell subsets in human beings (t4 = inducer cell; t5 = suppressor/cytotoxic cell), we have previously shown that most patients with multiple sclerosis (ms) have decreased t5 cells and an elevated t4:t5 ratio during periods of disease activity. we have recently studied three children with ms beginning at 4 years 11 months, 101/2 years, and 2 years 11 months of age. clinical symptoms included a relapsing brainstem syndrome in the first, devic syndrome and ... | 1982 | 6213188 |
| specific effect of the thyroid on testicular enzymes involved in carbohydrate metabolism. i. hypothyroidism. | the effect of thyroidectomy (tx) on some key enzymes involved in glycolysis and the hmp-shunt pathway were studied in the testis of pre-pubertal, pubertal and adult rats. hexokinase (hk) and phosphofructokinase (pfk) did not show any significant change in activity after tx. however, pyruvate kinase (pk) activity was reduced after tx in the testis of pre-pubertal animals although in pubertal and adult rats it was not affected. both glucose-6-phosphate dehydrogenase (g6p-dh) and 6-phosphogluconate ... | 1982 | 6213568 |
| the human autologous mixed lymphocyte reaction. iii. immune circuits. | the human autologous mixed lymphocyte reaction represents the proliferative response of t cells to determinants presented on autologous non-t cells. purified t4+ cells vigorously proliferate in response to stimulation by either (b + null) cells or m phi, whereas purified t8+ cells proliferate very little without a source of help. such help can be provided by mitomycin c-treated t4+ cells, which indicates that proliferation of t4+ cells is not necessary for the help. m phi suppress the human amlr ... | 1982 | 6213705 |
| cellular origin of interleukin 2 (il 2) in man: evidence for stimulus-restricted il 2 production by t4+ and t8+ t lymphocytes. | the human t cell subset(s) responsible for production of interleukin 2 (il 2) was investigated in the present study. for this purpose, highly purified t4+ and t8+ t lymphocytes were stimulated with mitogens and alloantigens. subsequently, culture supernatants were analyzed for il 2 activity in each of two assay systems: 1) proliferation of long-term t cell lines and 2) induction of cytotoxic effector cells from a resting t8+ population incubated in mlc. mitogen stimulation led to secretion of eq ... | 1982 | 6213706 |