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the effect of age on the morphometric sperm traits of domestic pigs (sus scrofa domestica).the experiment was conducted using 20 male domestic pigs, which were in 2 (equal-sized) age groups: under 14 months old and over 18 months old. at least 5 ejaculates from each male were taken, and in each ejaculate, morphometric measurements of 50 spermatozoa were made. the measured parameters were head area, head length and width, and flagellum length. for each ejaculate, the basic physical traits and the frequency of occurrence of developmental anomalies of the spermatozoa were examined. it wa ...200515809675
successional behavior and occurrence matrix of carrion-associated arthropods in the urban area of medellín, colombia.forensic entomology is a scientific tool applied to the study of insect or arthropod succession at the scene of a crime or that associated with an accident or natural death. interpreting this succession provides information to determine minimum and maximum limits of the postmortem interval (pmi), that is, the time between death and the discovery of the body. this study was carried out during the rainy season, from 27 october to 12 december 2002 in an urban area of the city of medellín, colombia. ...200515813558
macro anatomical investigations of the cranial cervical ganglion in domestic pig (sus scrofa domesticus).in this study, the left and right cranial cervical ganglia (ganglion cervicale craniale) of eight young (four male, four female) domestic pigs weighing around 70-80 kg were inspected macro anatomically. the cranial cervical ganglion (ccg) was found cranio-ventrally of the distal ganglion of the vagus nerve, medial of the jugular process extremity, ventral of the atlas, dorsal of the epiglottis base and medial of the common root (cr) established by the internal carotid and occipital arteries. the ...200515929737
optimization of a blood pool contrast agent injection protocol for mr angiography.to design an ideal first-pass profile for mr angiography (mra) by optimizing a multiphasic injection protocol based on two experimental animal models.200515834909
domesticated pigs in eastern indonesia. 200516020714
vaginal contraceptive activity of a chelated vanadocene.bis(cyclopentadienyl) complexes of vanadium (iv) or vanadocenes are rapid and potent inhibitors of human sperm motility with potential as a new class of contraceptive agents. this study sought to determine the vaginal contraceptive activity of vanadocene dithiocarbamate (vddtc), a stable vanadocene (iv)-chelated complex, using the standard rabbit model as well as the domestic pig as a physiologically relevant animal model for contraception. in experiment i, ovulating new zealand white does in su ...200516022855
comparative genetic characterization of porcine circovirus type 2 samples from german wild boar populations.pcv-2 is involved in "postweaning multisystemic wasting syndrome" (pmws), "porcine dermatitis and nephropathy syndrome" (pdns), respiratory and reproductive disorders, and thereby plays a crucial role in today's swine production world-wide. the virus is apparently ubiquitous in domestic pigs and has also been demonstrated in wild pigs. up to now, a characterization of pcv-2 samples from wild pigs, which might help to estimate the possible role of wild pigs as sources of domestic pig infection, h ...200516023798
phylogenetic analysis of classical swine fever virus (csfv) field isolates from outbreaks in south and central america.to date, there is little information concerning the epidemiological situation of classical swine fever (csf) in the americas. besides summarizing the available data, genotyping of isolates from outbreaks in domestic pigs in several countries of south and central america was performed. for this, a 190 base fragment of the e2 envelope glycoprotein gene was used. european strains and isolates, and historical isolates from the united states (us) were included for comparison. in contrast to the situa ...200515845261
assessment of in vivo mr imaging compared to physical sections in vitro--a quantitative study of brain volumes using stereology.the object of the present study was to compare stereological estimates of brain volumes obtained in vivo by magnetic resonance imaging (mri) to corresponding volumes from physical sections in vitro. brains of ten domestic pigs were imaged using a 3-t scanner. the volumes of different brain compartments were obtained from mr images by two observers and from physical sections using the cavalieri estimator in combination with point counting. paired t tests revealed no significant differences betwee ...200515862205
experimental study on fluorescent microspheres as a tracer for sentinel node detection.common tracers for sentinel node navigation surgery are blue dye and technetium-99m-labelled colloids. however, in most esophageal or lung cancer patients, it is impossible to detect the sentinel node among mediastinal nodes by blue dye because of the anthracotic pigmentation of mediastinal nodes. the use of technetium-99m-labelled colloids requires a special facility, while a large hot-spot at the injection site prevents detection of the sentinel node around the primary lesion. to overcome thes ...200515868914
transscleral implantation and neurophysiological testing of subretinal polyimide film electrodes in the domestic pig in visual prosthesis development.loss of photoreceptor function is responsible for a variety of blinding diseases, including retinitis pigmentosa. advances in microtechnology have led to the development of electronic visual prostheses which are currently under investigation for the treatment of human blindness. the design of a subretinal prosthesis requires that the stimulation device should be implantable in the subretinal space of the eye. current limitations in eye surgery have to be overcome to demonstrate the feasibility o ...200515876656
comparison of male and female meiotic segregation patterns in translocation heterozygotes: a case study in an animal model (sus scrofa domestica l.).the comparison of male and female meiotic segregation patterns for individuals carrying identical reciprocal translocations has been rarely reported in mammalian species. the main comparative study involving males and females with comparable genetic background has been performed in the mouse. swine is another relevant animal model species for meiotic studies. here we present the segregation patterns determined for sows carrying one of the two following reciprocal translocations: 38, xx, rcp(3;15 ...200515878917
a dna vaccine expressing the e2 protein of classical swine fever virus elicits t cell responses that can prime for rapid antibody production and confer total protection upon viral challenge.immunization of domestic pigs with a dna vaccine expressing the complete e2 protein of classical swine fever virus (csfv) conferred total protection against a severe viral challenge. immunization with three doses of plasmid pcdna3.1/e2 elicited a consistent and specific, mhc class ii restricted t cell response in the three domestic pigs analyzed, in the absence of detectable anti-csfv antibodies in serum. upon challenge specific t cell responses were boosted in the three vaccinated pigs, and a r ...200515882536
[the development of the gene pool in the microevolution of sus scrofa and the role of heterozygosity in the breed-forming process].the gene pool formation of the modem domestic breeds of pig sus scrofa domestica and their genesis based on hybridization of wild ancestral forms of the european and asian origin were studied using molecular immunogenetic methods. males of the european and central asian s. scrofa subspecies (s. s. scrofa and s. s. nigripes were hybridized with domestic pigs of the swedish landrace and vietnamese black masked breeds. in addition, we examined the genotypic structure of 65 wild, aboriginal, and loc ...200515909917
a report of decomposition rates of a special burial type in edmonton, alberta from an experimental field study.regional studies that examine decomposition rates of certain faunal remains can help to determine time since death. forensic anthropologists have long used qualitative decomposition data, but linking these to more quantitative data could improve time since death estimations. experiments were developed in which domestic pigs (sus scrofa) were buried with varying characteristics then excavated and observed over a period of 15 months in edmonton, alberta. data recorded after two weeks, five weeks, ...200515932100
collagenase promotes the cellular responses to injury and wound healing in vivo.this study focuses on the growth-promoting and migration-enhancing role that clostridial collagenase plays in vitro and in vivo.200516921413
histochemical properties of fibre types in muscles of wild and domestic pigs and the effect of growth rate on muscle fibre properties.this study reports the results of a comparison of the histochemical properties of various types of porcine muscles in wild and domestic pigs. in domestic pigs, the influence of growth rates on these properties were studied in five muscles: longissimus dorsi (ld), semimembranosus (sm), gluteus superficialis (gs), infra spinam (is) and masseter (mas). the growth rate was expressed as: (i) live weight (lw) at 165±2 days and (ii) daily lw gain from day 88 to day 165 (dg) on the experimental diet. ld ...200422061529
an investigation into natural resistance to african swine fever in domestic pigs from an endemic area in southern africa.a population of domestic pigs in northern mozambique with increased resistance to the pathogenic effects of african swine fever (asf) virus was identified by the high prevalence of circulating antibodies to asf virus. an attempt was made to establish whether the resistance in this population was heritable. some of these pigs were acquired and transported to a quarantine facility and allowed to breed naturally. offspring of the resistant pigs were transferred to a high security facility where the ...200415861893
foraging behaviour in domestic pigs (sus scrofa): remembering and prioritizing food sites of different value.this experiment investigated whether domestic pigs can remember the locations of food sites of different relative value, and how a restricted retrieval choice affects their foraging behaviour. nine juvenile female pigs were trained to relocate two food sites out of a possible eight in a spatial memory task. the two baited sites contained different amounts of food and an obstacle was added to the smaller amount to increase handling time. on each trial, a pig searched for the two baited sites (sea ...200415871038
radio-frequency tissue ablation in liver trauma: an experimental study.the liver is the most frequently injured intra-abdominal organ. radio-frequency tissue ablation (rfa) with cooled tip electrodes is here experimentally used for the treatment of liver trauma. a grade iii and a grade iii to iv trauma each were produced in the livers of 10 domestic pigs. rfa was applied around the sites of injury until hemostasis was achieved. the animals were sacrificed at 0, 3, 7, 14, and 21 days and examined. the livers were subjected to histologic and radiologic examination. t ...200415586512
transepicardial autologous bone marrow-derived mononuclear cell therapy in a porcine model of chronically infarcted myocardium.cell therapy is becoming a viable strategy to improve revascularization and myocardial function after myocardial injury. we evaluated the effect of bone marrow-derived mononuclear cell (bmmnc) transplantation on collateral vessel development and myocardial function in a porcine model of chronically infarcted heart.200415721847
renal tolerance to prolonged warm ischemia time in a laparoscopic versus open surgery porcine model.to our knowledge the effects of renal warm ischemia (wi) during laparoscopic vs open surgery have not been investigated. decreased renal blood flow during pneumoperitoneum may precondition the kidney to tolerate longer wi time. traditionally 30 minutes has defined the limit of renal wi time in open surgery. however, recent reports show renal function recovery at wi times of 45 to 120 minutes. we assessed renal function recovery after prolonged wi during laparoscopic vs open surgery in a solitary ...200415538293
lung biopsy with a 12-gauge cutting needle is possible using an insertion sheath in animal models.the volume of lung tumor core biopsy specimens has been restricted because of concerns for complications such as bleeding and air leakage. in this animal experiment, we investigated the possibility of larger bore biopsies through the peripheral lung parenchyma.200415561575
[prevalence of hemorrhagic fever with renal syndrome virus in domestic pigs: an epidemiological investigation in shandong province].to investigate the epidemiological significance of hemorrhagic fever with renal syndrome virus (hfrsv) infection in domestic pigs in shandong province, and study the role of domestic pigs in the prevalence of hfrs.200415567780
routes of transmission of swine hepatitis e virus in pigs.hepatitis e virus (hev) is believed to be transmitted by the fecal-oral route in pigs. to date, in experiments, hev has been transmitted successfully only by the intravenous or intrahepatic route. to assess the route of hev transmission, 27 pigs were separated into nine groups of three pigs. positive-control pigs were inoculated intravenously with swine hev and served as the source of hev for the other groups. uninoculated contact pigs were placed in the positive-control group. on three consecut ...200415528694
[analysis of presence and difference of sequence of porcine endogenous retrovirus in dna genomes from peripheral blood white cells of pig and mrna in tissues from mini-pigs].the aim is to investigate biological features of porcine endogenous retrovirus (perv) of pigs in china and to provide basic parameters for evaluation of biological safety of xenotransplantation from pig to human. in this study, basic biologic features of perv of dna of peripheral blood white cells of 12 species of domestic pigs in china were examined by polymerase chain reaction; analysis of difference of perv gene sequence was investigated by sliver-stained single stranded conformational polymo ...200415636362
clinical aspects of percutaneous poisoning by the chemical warfare agent vx: effects of application site and decontamination.o-ethyl s-(2-diisopropylaminoethyl) methylphosphonothioate (vx) is an extremely toxic organophosphate nerve agent that has been weaponized and stockpiled in a number of different countries, and it has been used in recent terrorist events. it differs from other well-known organophosphate nerve agents in that its primary use is as a contact poison rather than as an inhalation hazard. for this reason, we examined the effects of application site and skin decontamination on vx toxicity in anesthetize ...200415605929
spatial and temporal patterns of human african trypanosomosis (hat) transmission risk in the bipindi focus, in the forest zone of southern cameroon.vector control is an effective and cost-efficient way to disrupt the transmission of human african trypanosomosis (hat); it has nonetheless been little used to date in the disease's foci. with the aim to target trapping more precisely and to develop an optimized vector control system, a transmission risk index was used in the hat focus of bipindi, in the forest zone of southern cameroon. the authors used a simplified version of the index originally developed by laveissière et al. in 1994. the ca ...200415631068
[analysis of gene expression profiles in heart tissues from two breeds of pigs].to study the expression profiles in the heart tissues of domestic pigs,five un-normalized cdna libraries from danish adult female landrace heart, biceps femoris, aorta, left ventricle tissues and chinese adult female erhualian heart tissue were constructed. totally 35 180 expressed sequence tags (ests) were randomly sequenced. using functional clustering and statistic methods, three gene clusters and 47 genes highly expressed in landrace heart tissue were found. these genes are corresponding to ...200415490873
diurnal rhythms of pinealocyte ultrastructure, pineal serotonin content and plasma melatonin level in the domestic pig.the study was conducted to investigate diurnal changes in pinealocyte ultrastructure, pineal serotonin content and plasma melatonin concentration in the domestic pig. the immature pigs (n=24) were kept under a cycle of 12 h light : 12 h dark, with a photophase between 0800 and 2000. during the photophase the animals were exposed to direct sunlight. after four weeks the gilts were slaughtered at 0900, 1400, 2100 and 0200. the pineals were removed and divided into two parts - one for quantitative ...200415493576
a novel intestinal anastomotic device in a porcine model.the purpose of this study was to evaluate a novel, intraluminally deployed anastomotic device (ad). a survival study was conducted in 18 farm pigs. one early subject was excluded and replaced due to premature expiration. six animals were placed in 1 of 3 cohorts, with euthanasia and ad explantation planned at 2, 4, and 6 weeks. a distal small intestinal side-side [functional end-end] anastomosis using the ad was performed via midline laparotomy. fluoroscopy with double-contrast dilute barium and ...200415481291
co-circulation of two genetically distinct viruses in an outbreak of african swine fever in mozambique: no evidence for individual co-infection.in 1998, domestic pigs originating from villages within a 40 km radius of ulongwe in the northern tete province of mozambique were held in a quarantine facility for a 3-month period prior to their importation into south africa. eight of a total of 25 pigs died within the first 3 weeks of quarantine of what appeared clinically and on post mortem examination to be african swine fever (asf). organs were collected and preserved in formol-glycerosaline and the presence of asf virus in these specimens ...200415504588
first record of trichinella pseudospiralis in the slovak republic found in domestic focus.infection of trichinella spp. is widespread among wildlife in slovakia and the red fox (vulpes vulpes) is the main reservoir of trichinella britovi. trichinella spiralis has been rarely documented in sylvatic and domestic animals of this country. during routine examination of domestic pigs at the slaughter, trichinella larvae were detected by artificial digestion in a domestic pig of a large-scale breeding farm in eastern slovakia. the parasite has been identified by molecular (pcr) and biochemi ...200415725537
differential effect of experimental hypertension and hypercholesterolemia on adventitial remodeling.intima-media remodeling, as frequently assessed by changes in the external elastic lamina-to-lumen area (eella), is well-described in coronary artery disease in contrast to adventitial remodeling, especially in the early disease stage.200415591225
laser-assisted vascular end to end anastomosis of elastin heterograft to carotid artery with an albumin stent: a preliminary in vivo study.laser-assisted end to end vascular anastomosis of an elastin heterograft to native artery may prevent problems associated with currently available vascular synthetic grafts and conventional suture anastomosis.200415389735
intraocular lenses, bacterial adhesion and endophthalmitis prevention: a review.postoperative endophthalmitis following intraocular lens (iol) implantation is still one of the most feared complications of cataract surgery. bacterial adhesion to iols during their insertion is a prominent etiological factor. polypropylene was the first biomaterial that allowed this relation of cause and effect to be proven. following adhesion, bacteria replicate, congregate and form multiple layers of microcolonies which actually represent the basic structural unit of the biofilm. the bacteri ...200415472388
molecular investigation of hepatitis e virus infection in domestic and miniature pigs used for medical experiments.hepatitis e virus (hev) infection is highly prevalent among domestic pigs in japan. it has been reported that pig handlers such as farmers and veterinarians are at increased risk of contracting hev infection. pigs are regarded as the most acceptable candidate animals for xenotransplantation and, recently, they are being used as experimental animals.200415479459
phenotypic features of the domestic pigs bred in the roman settlements of pompeii and caralis.a reconstruction of the morphological features of domestic pigs from two roman settlements is here suggested by means of the study of skeletal and dental remains, with the aim at evaluating their degree of selection in comparison with wild boars. material was formed by 111 bone and tooth fragments and was uncovered during the excavations of polybius' house in pompeii and of roman buildings in the neighbourhood of caralis harbour (sardinia). the remains underwent morphological examination. the er ...200415481159
attempts to reproduce vacuolar myelinopathy in domestic swine and chickens.avian vacuolar myelinopathy (avm) was first recognized as a cause of bald eagle (haliaeetus leucocephalus) mortality in 1994 in arkansas (usa) and has since caused over 90 bald eagle and numerous american coot (fulica americana) mortalities in five southeastern states. the cause of avm remains undetermined but is suspected to be a biotoxin. naturally occurring avm has been limited to wild waterbirds, raptors, and one species of shorebird, and has been reproduced experimentally in red-tailed hawk ...200415465715
cloning of host-ptp: the only example of a protein-tyrosine-phosphatase the function of which has been lost between rodent and human.protein-tyrosine-phosphatases (ptp-ases), in concert with protein tyrosine kinases, control various biological activities such as cell growth and differentiation. in rodents, around 40 ptp-ases have been described. functional orthologue for each of these ptp-ases have been identified in human, except for ost-ptp. ost-ptp is a transmembrane ptp-ase with a restricted tissue distribution. in silico analysis on public sequence databases reveals a human ost-ptp gene orthologue that encompasses 21 kb ...200415358244
investigations into growth and differentiation in the cartilage of the condylar process in the domestic pig. a quantitative study of endochondral cartilage growth and cell distribution.the aim of this study was to examine the extent to which the ratio of the endochondral growth components cell volume increase to matrix production was subject to age-dependent and animal-specific changes per cell in the condylar cartilage of the domestic pig. in addition, it was to be investigated whether the cell distribution in the condylar cartilage and thus the tissue differentiation changes as one aspect of the aging process and whether the differentiation differs from that of primary hyali ...200415378192
laser-assisted end-to-end vascular anastomosis of elastin heterograft to carotid artery with an albumin stent in vivo.laser-assisted end-to-end vascular anastomosis of an elastin heterograft to native artery may avoid the problems associated with currently available vascular synthetic grafts and conventional suture anastomosis.200415345171
measurement of portal blood and transjugular intrahepatic portosystemic shunt flow with use of a retrograde thermodilutional catheter.direct blood flow measurement in the portal vein (pv) and in transjugular intrahepatic portosystemic shunts (tips) holds potential as a means of optimizing shunt creation and predicting outcomes. a retrograde thermodilutional flow measurement catheter was recently designed. the purpose of this study was to validate and optimize the technique of blood flow measurement in the pv with use of this catheter in a swine model of tips.200415466797
epidemiological study on porcine circovirus type 2 (pcv2) infection in the european wild boar (sus scrofa).porcine circovirus type 2 (pcv2) is considered as the causative agent of postweaning multisystemic wasting syndrome (pmws) in domestic pigs, where the virus is ubiquitous as evidenced by serological surveys. we present the results of the first nationwide sero-survey on the presence of pcv2 antibodies in european wild boars, and report the first pmws case in a wild boar from spain. sera from 656 hunter harvested wild boars from 45 different geographical sites and 22 additional imported animals we ...200415099500
novel herpesviruses of suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses.five novel herpesviruses were identified in suid species from africa (common warthog, phacochoerus africanus) and south-east asia (bearded pig, sus barbatus; babirusa, babyrousa babyrussa) by detection and analysis of their dna polymerase genes. three of the novel species, p. africanus cytomegalovirus 1, p. africanus lymphotropic herpesvirus 1 (pafrlhv-1) and s. barbatus lymphotropic herpesvirus 1 (sbarlhv-1), were closely related to known beta- (porcine cytomegalovirus) and gammaherpesviruses [ ...200415039528
adenoviral catheter-mediated intramyocardial gene transfer using the mature form of vascular endothelial growth factor-d induces transmural angiogenesis in porcine heart.it is unclear what is the most efficient vector and growth factor for induction of therapeutic vascular growth in the heart. furthermore, the histological nature of angiogenesis and potential side effects caused by different vascular endothelial growth factors (vegfs) in myocardium have not been documented.200414967735
laparoscopic partial nephrectomy using holmium laser in a porcine model.to evaluate the utility of the holmium laser for partial nephrectomy in a porcine model.200414974664
comparison of systemic responses of radiofrequency ablation, cryotherapy, and surgical resection in a porcine liver model.the degree of systemic response after hepatic radiofrequency ablation (rfa) has not been well investigated.200415231521
methylprednisolone enhances contraction of porcine femoral head epiphyseal arteries.the pathomechanism of steroid-induced femoral head necrosis is thought to be disturbed femoral head blood flow. we hypothesize that methylprednisolone increases vasocontraction of femoral head epiphyseal arteries, thereby reducing femoral head blood flow. nine immature female domestic pigs were randomly selected from a group of 18 to receive 24-hour methylprednisolone treatment, whereas the nine remaining pigs received the placebo control in a blinded fashion. after sacrifice, lateral epiphyseal ...200415232435
the epidemiological investigation of trichinella infection in brown rats (rattus norvegicus) and domestic pigs in croatia suggests that rats are not a reservoir at the farm level.whether the brown rat (rattus norvegicus) is a reservoir of trichinella spp. infection or merely an accidental host, which may be vector of trichinella spp., continues to be debated. we estimated the prevalence of trichinella sp. infection in brown rat populations and in domestic pigs in 2 villages in croatia, where trichinella sp. infection in pigs has been endemic in the past 10 yr. trichinella spiralis larvae, identified by a multiplex polymerase chain reaction analyses, were the only species ...200415270124
closure of the cystic duct during laparoscopic cholecystectomy with a new feedback-controlled bipolar sealing system in case of biliary obstruction--an experimental study in pigs.the excellent results of new devices like the new bipolar feedback-controlled sealing system (ligasure) for closure of blood vessels encouraged surgeons to use these instruments for other structures like bile ducts. the aim of this study was to evaluate the feasibility of closure of cystic duct in case of biliary obstruction.200415239216
monoclonal antibody-based immunohistochemical diagnosis of malaysian nipah virus infection in pigs.formalin-fixed, paraffin wax-embedded tissues of three malaysian farm pigs naturally infected with nipah virus were used to investigate the value of anti-nipah virus mouse monoclonal antibodies (mabs) and rabbit polyclonal antibody for immunohistochemical diagnosis. mabs 11f6 and 12a5 gave intense immunolabelling in lung tissue that had been fixed in 10% neutral buffered formalin for about 4 years, whereas the reactivity of mabs 13a5 and 18c4 and polyclonal antibody was reduced significantly by ...200415276859
effects of hypertonic versus isotonic infusion therapy on regional cerebral blood flow after experimental cardiac arrest cardiopulmonary resuscitation in pigs.to evaluate the effects of hypertonic, isooncotic, and isotonic infusion therapy on cerebral blood flow (cbf) during and after cardiopulmonary resuscitation (cpr) from experimental cardiac arrest (ca).200415451589
an anaesthetic protocol in the young domestic pig allowing neuromuscular blockade for studies of cardiac function following cardioplegic arrest and cardiopulmonary bypass.neuromuscular blockade should, for ethical reasons, not be allowed in animal experiments unless the use is strongly motivated. beforehand, the anaesthetic protocol must be documented without muscle relaxation in the species studied. documentation is difficult to obtain from the scientific literature. when focusing on cardiac function over time, in particular, the ideal anaesthetic protocol should cause no or minor alterations in cardiac variables.200415352961
feasibility of stent placement in carotid arteries with real-time mr imaging guidance in pigs.all examinations were performed with approval from the institutional animal care and use committee of columbia university. to assess the feasibility of real-time magnetic resonance (mr) imaging-guided neurovascular intervention in a swine model, the authors placed stents in the carotid arteries of five domestic pigs. seven-french vascular sheaths were placed in the target carotid arteries via femoral access by using active mr tracking. ten nitinol stents (8-10 x 20-40 mm) were successfully deplo ...200415591432
transfemoral catheterization of carotid arteries with real-time mr imaging guidance in pigs.all procedures and protocols were approved by the institutional animal care and use committee of columbia university. to determine whether transfemoral catheterization of the carotid arteries can be performed entirely with real-time magnetic resonance (mr) imaging guidance, the authors catheterized the carotid arteries in six domestic pigs by using active-tracking catheters and guidewires and mr tracking software created for neurovascular procedures. the carotid arteries were successfully cathet ...200415591433
classical swine fever virus strain 'c'. how long is it detectable after oral vaccination?to determine the persistence period of c-strain vaccine virus in immunized animals, domestic pigs and wild boars were vaccinated orally and killed on different days post vaccinationem (dpv). tissue samples were taken at necropsy from both species for detection of c-strain virus. from domestic pigs nasal swabs and faeces were also collected. during the investigation period (2-12 dpv) vaccine virus could never be detected in nasal secretions and in faeces of vaccinated domestic pigs. in contrast, ...200415458487
smooth-muscle regeneration after electrosurgical endopyelotomy in a porcine model as confirmed by electron microscopy.endopyelotomy is the preferred treatment for ureteropelvic junction (upj) obstruction because of its short operating time, limited morbidity, fast recovery, and reasonable efficacy. we used tissue and immunohistochemistry staining and electron microscopy to look at the muscle regeneration following an endopyelotomy incision in a porcine model.200415801366
inhibitory g protein overexpression provides physiologically relevant heart rate control in persistent atrial fibrillation.the need for new treatment strategies for cardiac arrhythmias has motivated our continuing development of gene therapeutic options. previously, we reported a decreased heart rate in an acute model of atrial fibrillation after atrioventricular nodal gene transfer. here, we expand those observations to persistent atrial fibrillation and severe heart failure.200415505099
assessment of subacute inflammatory and proliferative response to coronary stenting in a porcine model by local gene expression studies and histomorphometry.the aim of the study was to analyse inflammatory and proliferative response early after coronary stenting by angiography, histomorphometry and local gene expression analysis using quantitative rt-pcr. therefore, eight german domestic pigs underwent stenting of the left coronary artery. selective coronary angiography was performed after 14 days. explanted coronary arteries were examined histomorphometrically after methacrylate-embedding. snap-frozen samples were examined for local gene expression ...200414615159
pcr identification of beef, sheep, goat, and pork in raw and heat-treated meat mixtures.a pcr assay has been developed for the specific and qualitative detection of pork (sus scrofa domesticus), beef (bos taurus), sheep (ovis aries), and goat (capra hircus) in raw and heat-treated meat mixtures. a forward common primer was designed on a conserved dna sequence in the mitochondrial 12s ribosomal rna gene (rrna), and reverse primers were designed to hybridize on species-specific dna sequences of each species considered. the different sizes of the species-specific amplicons, separated ...200414717369
long-term retention of endoscopically placed hydrogel prostheses at the lower esophageal sphincter in pigs.using flexible endoscopic methods, hydrogel prostheses can be safely placed, retained for long periods of time, and removed from the esophageal submucosa of pigs. this new technique may have future applications in the treatment of gastroesophageal reflux disease in selected situations.200414752644
[trichinellosis outbreaks in spain (1990-2001)].trichinellosis is a helminth infection having a wide geographical distribution. it is acquired by ingestion of raw or undercooked meat infected with trichinella spp. the present work reviews the human trichinellosis outbreaks recorded in spain during 1990-2001, taking into account both the geographical distribution and source of the infection.200414756987
how long can we preserve the pulmonary graft inside the nonheart-beating donor?the use of lungs from nonheart-beating donors (nhbd) might significantly alleviate the organ shortage. extending the preharvest interval in nhbd would facilitate distant organ retrieval. we hypothesized that prolonged topical cooling inside nhbd after 60 minutes of initial warm ischemia would not affect the pulmonary graft.200414759412
effect of food consistency on temporomandibular joint morphology: an experimental study in pigs.to investigate whether there are any correlations between increased masticatory loading, degree of tooth wear, and the size, form, and macroscopic surface of the temporomandibular joints (tmjs).200415022535
comprehensive evaluation of ureteral healing after electrosurgical endopyelotomy in a porcine model: original report and review of the literature.endopyelotomy (ep) has yet to equal the success achieved with open dismembered pyeloplasty. to understand better the ureteral response to ep we performed a timed histopathological evaluation of the porcine ureter after acucise (applied medical, rancho santa margarita, california) ep.200414713843
respiratory changes during prolonged increased intra-abdominal pressure in pigs.increased intra-abdominal pressure (iap) elevates thoracic pressure and airway pressures and reduces lung compliance in humans and laboratory animals. we studied respiratory alterations and arterial blood gas changes in pigs with iap maintained at 20 mmhg or 30 mmhg for 3 h.200415025609
prevalence of porcine endogenous retrovirus in domestic pigs in japan and its potential infection in dogs xenotransplanted with porcine pancreatic islet cells.the prevalence of porcine endogenous retrovirus (perv) proviral dna among various pig breeds raised in japan was investigated by polymerase chain reaction (pcr). moreover, potential infection of perv was investigated by pcr and reverse transcriptase-polymerase chain reaction (rt-pcr) in experimentally induced diabetic dogs (n=5) implanted with the diffusion chamber type bio-artificial endocrine pancreas (bio-aep) containing porcine pancreatic endocrine (pe) cells. no immunosuppressant was used a ...200415031539
betaine improves growth, but does not induce whole body or hepatic palmitate oxidation in swine (sus scrofa domestica).dietary betaine may reduce carcass fat in growing pigs. we explored the effects of betaine on short-term growth and in vivo and in vitro fatty acid oxidation. pigs were housed in metabolism crates and fed diets containing either 0% (control), 0.125% or 0.5% betaine at 80% of ad libitum energy intake. fatty acid oxidation was measured during intravenous infusions of 1-(13)c-palmitate and in hepatocytes incubated in the presence or absence of betaine and carnitine. co2 and palmitate isotopic enric ...200414720598
changes of lymphatic flow in case of pancreatic duct obstruction in the pig--as a model of pancreatic cancer.to investigate the lymph network change in pancreatic duct obstruction in pigs as a model of pancreatic cancer invading pancreatic duct, six domestic pigs weighing 17-40 kg underwent surgery as protocol. two of them were controls, and the others underwent ligation of the pancreatic duct as a model of ductal obstruction. a ch 40 and lipiodol mixture was injected in their pancreas at 7 or 21 days after first operation. radiographic examination had been also performed. five or 14 days later, they w ...200415000259
experimental disc degeneration due to endplate injury.the aim of this study was to create an experimental model of disc degeneration that closely mimicked human disc degeneration. in six domestic pigs, an l4 cranial endplate perforation into the nucleus pulposus was made. three months postoperatively, compressive testing was performed on the l2-l4 motion segments, and intradiscal pressure was measured in the intervening discs. histochemical and morphologic examinations were made on the excised degenerated and adjacent discs. a significant reduction ...200414734978
acute myocardial infarction: contrast-enhanced multi-detector row ct in a porcine model.to assess the role of contrast material-enhanced retrospectively electrocardiographically (ecg) gated multi-detector row computed tomography (ct) in the detection of acute myocardial infarction in a porcine model of total coronary occlusion.200415118118
uptake of 111in-z2d3 on spect imaging in a swine model of coronary stent restenosis correlated with cell proliferation.small targets such as cell proliferation in the coronary arteries may potentially be detected with single-photon imaging using high-radiotracer-specific activity. we hypothesized that an antibody linked to polymers to increase specific radioactivity can be visualized on spect images and that counts in the target will correlate with the strength of the biologic signal.200414960651
description and evaluation of a ventriculo-coronary artery bypass device that provides bi-directional coronary flow.the objective of this study was to assess acute patency of a new myocardial revascularization device that connects the left ventricular cavity to a coronary artery (termed ventriculo-coronary artery bypass, vcab) thereby providing proximal and distal blood flow from the site of the anastomosis.200414690731
spatial comparison between wall shear stress measures and porcine arterial endothelial permeability.a better understanding of how hemodynamic factors affect the integrity and function of the vascular endothelium is necessary to appreciate more fully how atherosclerosis is initiated and promoted. a novel technique is presented to assess the relation between fluid dynamic variables and the permeability of the endothelium to macromolecules. fully anesthetized, domestic swine were intravenously injected with the albumin marker evans blue dye, which was allowed to circulate for 90 min. after the an ...200414715506
spontaneous gasping generates cardiac output during cardiac arrest.to measure stroke volumes coincident with spontaneous gasping during untreated ventricular fibrillation and to evaluate the effects of gasping.200414707585
functional and phylogenetic analyses of a melanocortin-4 receptor mutation in domestic pigs.the melanocortin-4 receptor (mc4r), a g protein-coupled seven-transmembrane receptor, which is expressed in the brain, plays an important role in the control of mammalian energy homeostasis. a missense mutation (asp298asn) was identified in the porcine mc4r gene, which is associated with growth and food intake traits. the asn298 mutation occurs within a highly conserved motif, npliy, of all members of g protein-coupled receptors; whereas, asp298 is conserved in all five melanocortin receptor sub ...200414732454
emerging encephalitogenic viruses: lyssaviruses and henipaviruses transmitted by frugivorous bats.three newly recognized encephalitogenic zoonotic viruses spread from fruit bats of the genus pteropus (order chiroptera, suborder megachiroptera) have been recognised over the past decade. these are: hendra virus, formerly named equine morbillivirus, which was responsible for an outbreak of disease in horses and humans in brisbane, australia, in 1994; australian bat lyssavirus, the cause of a severe acute encephalitis, in 1996; and nipah virus, the cause of a major outbreak of encephalitis and p ...200415119765
[histological investigation of the micromorphological effects of the application of a laser needle--results of an animal experiment].in an experimental animal study (sus scrofa domesticus) we investigated the effects of the new technique of laser needle stimulation (wavelength: 685 nm; energy density: 4.6 kj/cm2 per point; application duration: 20 min). the results revealed changes in microcirculatory parameters of the skin resulting in an increase in blood flow. however, the quality and intensity of the laser light did not induce micromorphological alterations in the skin.200415032490
biological transmission of vesicular stomatitis virus (new jersey serotype) by simulium vittatum (diptera: simuliidae) to domestic swine (sus scrofa).the role of hematophagous arthropods in vesicular stomatitis virus (new jersey serotype; vsv-nj) transmission during epizootics has remained unclear for decades in part because it has never been shown that clinical or subclinical disease in a livestock host results from the bite of an infected insect. in this study, we investigated the ability of vsv-nj-infected black flies (simulium vittatum zetterstedt) to transmit the virus to domestic swine, sus scrofa l. experimental evidence presented here ...200414989350
3d pulmonary perfusion mri and mr angiography of pulmonary embolism in pigs after a single injection of a blood pool mr contrast agent.the purpose of this study was to assess the feasibility of contrast-enhanced 3d perfusion mri and mr angiography (mra) of pulmonary embolism (pe) in pigs using a single injection of the blood pool contrast gadomer. pe was induced in five domestic pigs by injection of autologous blood thrombi. contrast-enhanced first-pass 3d perfusion mri (te/tr/fa: 1.0 ms/2.2 ms/40 degrees; voxel size: 1.3 x 2.5 x 4.0 mm3; ta: 1.8 s per data set) and high-resolution 3d mra (te/tr/fa: 1.4 ms/3.4 ms/40 degrees; vo ...200414997336
prevalence of antibodies to hepatitis e virus among apparently healthy humans and pigs in bali, indonesia: identification of a pig infected with a genotype 4 hepatitis e virus.in indonesia where hepatitis e virus (hev) is believed to be highly endemic, only three outbreaks of hev transmission have been documented to date in restricted areas (west kalimantan and east java). a total of 1,115 serum samples collected from apparently healthy individuals in bali, lombok, and surabaya in indonesia in 1996 where epidemic hev transmissions have never been reported, were tested for igg class antibodies to hev (anti-hev). in bali, anti-hev was detected in 20% (54/276) of the tes ...200415042646
linkage disequilibrium in the domesticated pig.this study investigated the extent of linkage disequilibrium (ld) in two genomic regions (on chromosomes 4 and 7) in five populations of domesticated pigs. ld was measured with d' and tested for significance with the fisher exact test. effects of genetic (linkage) distance, chromosome, population, and their interactions on d' were tested both through a linear model analysis of covariance and by a theoretical nonlinear model. the overall result was that (1) the distance explained most of the vari ...200415082558
routine vascular control using the hem-o-lok clip in laparoscopic nephrectomy: animal study and clinical application.during laparoscopic nephrectomy (lpn), a stapling device is often used for vascular control, especially of the renal vein. herein, we report our experience using a polymer clip (hem-o-lok) for routine control of the vessels during lpn in the animal and clinical setting.200415006060
needle-based ablation of renal parenchyma using microwave, cryoablation, impedance- and temperature-based monopolar and bipolar radiofrequency, and liquid and gel chemoablation: laboratory studies and review of the literature.small renal tumors are often serendipitously detected during the screening of patients for renal or other disease entities. rather than perform a radical or partial nephrectomy for these diminutive lesions, several centers have begun to explore a variety of ablative energy sources that could be applied directly via a percutaneously placed needle-like probe. to evaluate the utility of such treatment for small renal tumors/masses, we compared the feasibility, regularity (consistency in size and sh ...200415006061
surgical construction of an in vivo carotid siphon model to test neurovascular devices.we developed an animal model to evaluate vascular trauma induced by endovascular devices that are proposed for the treatment of cerebrovascular diseases.200415113480
new metallic mr stents for artifact-free coronary mr angiography: feasibility study in a swine model.the objective of this study was to investigate the potential for artifact-free coronary magnetic resonance angiography (cmra) in the presence of dedicated metallic mr stents in vitro and in a swine model.200415087718
downregulation of n-cadherin in the neointima stimulates migration of smooth muscle cells by rhoa deactivation.the aim of the study was to analyze whether cadherin- and rho-family gtpases-mediated dynamic rearrangement of cell-cell adhesion play an important role during human arterial smooth muscle cell (hasmc) migration.200415023568
a novel population of calretinin-positive neurons comprises reelin-positive cajal-retzius cells in the hippocampal formation of the adult domestic pig.calretinin-containing neurons in the hippocampal formation, including the subiculum, presubiculum, parasubiculum, and entorhinal cortex, were visualized with immunocytochemistry. calretinin immunoreactivity was present exclusively in non-principal cells. the largest immunoreactive cell population was found in the outer half of the molecular layer of the dentate gyrus and in the stratum lacunosum-moleculare of ammon's horn. a proportion of these cells were also immunoreactive for reelin, a cajal- ...200415132437
interaction between a perfluorocarbon emulsion and radiographic contrast media.this study evaluated specially designed perfluorocarbon (pfc) emulsions as blood substitutes in case of induced ischemia of the left heart ventricle in healthy farm pigs. two hundred ml of perfluorocarbon emulsion were infused while 200 ml of blood were simultaneously drawn. radiographic contrast media were given to aid placement of balloon catheters in the left coronary artery. histopathological analysis showed that right heart failure caused the deaths of both pigs. particles (up to>3 micro) o ...200415152157
the effects of birth weight and postnatal growth patterns on fat depth and plasma leptin concentrations in juvenile and adult pigs.low birth weight is associated with altered adipose tissue deposition and regulation of leptin production. this study determined the effects of naturally occurring variations in birth weight in pigs on postnatal growth patterns, body fat depth and plasma leptin and other hormone concentrations. low (< 1.47 kg) and high (> 1.53 kg) birth weight piglets were studied at 3 months (juvenile; n= 47) and 12 months of age (young adult; n= 17). at each age, arterial and venous catheters were inserted und ...200415121800
immunogenicity and t cell recognition in swine of foot-and-mouth disease virus polymerase 3d.immunization of domestic pigs with a vaccinia virus (vv) recombinant expressing foot-and-mouth disease virus (fmdv) 3d protein conferred partial protection against challenge with infectious virus. the severity reduction of the clinical symptoms developed by the challenged animals occurred in the absence of significant levels of anti-3d circulating antibodies. this observation suggested that the partial protection observed was mediated by the induction of a 3d-specific cellular immune response. t ...200415110524
delayed portal vein thrombosis after experimental radiofrequency ablation near the main portal vein.portal venous blood flow may protect adjacent tumour cells from thermal destruction with radiofrequency ablation (rfa). this study aimed to investigate the local effect of rfa on the main portal vein branch, and the completeness of cellular ablation in its vicinity, with or without a pringle manoeuvre using a porcine model.200415122617
trichinellosis associated with bear meat--new york and tennessee, 2003.trichinellosis is a parasitic infection caused by tissue-dwelling trichinella roundworms and is associated traditionally with ingestion of pork from infected domestic swine. as a result of improvements in swine production, trichinellosis has declined steadily in the united states. however, infection also can result from eating the meat of wild animals. during 1997-2001, a total of 72 cases of trichinellosis (median: 12 cases annually; range: 11-23 cases) were reported to cdc; the majority of the ...200415254452
porcine wound models for skin substitution and burn treatment.skin regeneration is an important field of tissue engineering. especially in larger burns and chronic wounds, present treatments are insufficient in preventing scar formation and promoting healing. initial screening of potentially interesting products for skin substitution is usually done by in vitro tests. before entering the clinic, however, in vivo studies in immunocompetent animals are necessary to prove efficacy and provide information on safety aspects. we have obtained extensive experienc ...200414697858
splanchnic metabolism during gut ischemia and short-term endotoxin and hemorrhagic shock as evaluated by intravasal microdialysis.the splanchnic area is of considerable interest in different types of shock. to characterize the metabolic changes in the splanchnic region in response to different types of shock we used a model where shock-induced metabolic changes in the splanchnic region were studied by the use of intravasal microdialysis. 23 anesthetized domestic pigs were randomized into four groups: group i, serving as controls (n = 5); group ii, mesenteric ischemia for 180 followed by 120 min of reperfusion (n = 5); grou ...200415167688
in vivo porcine intradiscal pressure as a function of external loading.spinal loading during daily activity as it relates to the ability of the intervertebral disc to sustain its integrity has been a major issue in spinal research. the purpose of this investigation was to establish the relationship between the intervertebral disc pressure in the nucleus and the load applied to the motion segment in an in vivo porcine model.200415280761
ultrasound strain imaging of altered myocardial stiffness: stunned versus infarcted reperfused myocardium.in this study we evaluate the diastolic deformation of ischemic/reperfused myocardium and relate this deformation to tissue elastic properties.200415173032
in vitro coronary artery imaging with sixteen-slice helical computed tomography.to determine the benefits of submillimeter coronary computed tomography (ct) angiography in vitro.200415191099
detection and genetic characterization of porcine circovirus type 2 (pcv2) in pigs from croatia.porcine circovirus type 2 (pcv2) from the circoviridae family has recently been associated with two serious diseases of swine, post-weaning multisystemic wasting syndrome (pmws) and porcine dermatitis and nephropathy syndrome (pdns). during 2002, several outbreaks of clinical disease in pigs with weights ranging from 10 to 70 kg occurred on four farms in different locations in croatia. the signs were consistent with pmws and pdns. apart from progressive weight loss, pneumonia and/or diarrhoea, m ...200415196907
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