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studies on aedes aegypti resistance to some insecticides in the jazan district, saudi arabia.the present study provided information on the susceptibility status of the adult and larvae of aedes aegypti mosquitoes in jazan region of saudi arabia. bioassay tests were performed on adults and larvae by using who recommended concentrations and test kits. adults of ae. aegypti mosquitoes were exposed to test papers impregnated with lambda-cyhalothrin (0.05%), cyfluthrin (0.15%), deltamethrin (0.05%), permethrin (0.75%), fenitrothion (1%), bendiocarb (0.1%) and ddt (4%) insecticides. ae. aegyp ...201627363057
the eye of the tiger, the thrill of the fight: effective larval and adult control measures against the asian tiger mosquito, aedes albopictus (diptera: culicidae), in north america.the asian tiger mosquito, aedes albopictus (skuse), is a highly invasive container-inhabiting species with a global distribution. this mosquito, similar to other stegomyia species such as aedes aegypti (l.), is highly adapted to urban and suburban areas, and commonly oviposits in artificial containers, which are ubiquitous in these peridomestic environments. the increase in speed and amount of international travel and commerce, coupled with global climate change, have aided in the resurgence and ...201627354440
fitness of walbb wolbachia infection in aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion.wolbachia endosymbionts are potentially useful tools for suppressing disease transmission by aedes aegypti mosquitoes because wolbachia can interfere with the transmission of dengue and other viruses as well as causing deleterious effects on their mosquito hosts. most recent research has focused on the wmel infection, but other infections also influence viral transmission and may spread in natural populations. here, we focus on the walbb infection in an australian outbred background and show tha ...201626711515
larvicidal activity of catechin isolated from leucas aspera against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of plant origin my serve as an alternative biocontrol technique in the future. the aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extracts of leucas aspera on the fourth-instar larvae of aedes aegypti, anopheles stephensi, and culex quinquefasciatus. the larvae were exposed to fractions with concentrations ranging ...201626711450
development of a mosquito attractant blend of small molecules against host-seeking aedes aegypti.a mosquito's dependence on olfaction in the hunt for human host could be efficiently exploited to protect humans from mosquito bites. the present study is undertaken to make the most attractant compound blend for aedes aegypti mosquitoes to lure them to traps. eleven molecules (m1-m11) at different dilutions were screened for attractancy against non-blood-fed adult female mosquitoes in an olfactometer. the results showed that the attractancy was dependent on both the chemical nature of the molec ...201626693718
differentiation of aedes aegypti and aedes notoscriptus (diptera: culicidae) eggs using scanning electron microscopy.aedes notoscriptus and aedes aegypti are both peri-domestic, invasive container-breeding mosquitoes. while the two potential arboviral vectors are bionomically similar, their sympatric distribution in australia is limited. in this study, analyses of ae. aegypti and ae. notoscriptus eggs were enabled using scanning electron microscopy. significant variations in egg length to width ratio and outer chorionic cell field morphology between ae. aegypti and ae. notoscriptus enabled distinction of the t ...201626845557
characterization of enzymatic profiles of aedes aegypti strains from the state of rio grande do norte, brazil.this study was conducted in four strains of aedes aegypti mosquitoes to evaluate the enzymatic activity profiles in the city of mossoró, rio grande do norte, and correlate them with biochemical mechanisms of resistance to insecticides. mosquitos were used to quantify the following detoxification enzymes: mixed-function oxidase (mfo), pnpa-esterase (pnpa-est), and acetylcholinesterase (ache). the profiles were compared statistically with profiles from the rockefeller strain, through the kruskal-w ...201626816185
aedes aegypti (l.) in latin american and caribbean region: with growing evidence for vector adaptation to climate change?within latin america and the caribbean region the impact of climate change has been associated with the effects of rainfall and temperature on seasonal outbreaks of dengue but few studies have been conducted on the impacts of climate on the behaviour and ecology of aedes aegypti mosquitoes.this study was conducted to examine the adaptive behaviours currently being employed by a. aegypti mosquitoes exposed to the force of climate change in lac countries. the literature on the association between ...201626796862
effect of cymbopogon citratus (lemongrass) and syzygium aromaticum (clove) oils on the morphology and mortality of aedes aegypti and anopheles dirus larvae.cymbopogon citratus (lemongrass) and syzygium aromaticum (clove) oils were evaluated to determine mortality rates, morphological aberrations, and persistence when used against third and fourth larval instars of aedes aegypti and anopheles dirus. the oils were evaluated at 1, 5, and 10 % concentrations in mixtures with soybean oil. persistence of higher concentrations was measured over a period of 10 days. for ae. aegypti, both plant oils caused various morphological aberrations to include deform ...201626796022
delivery of chitosan/dsrna nanoparticles for silencing of wing development vestigial (vg) gene in aedes aegypti mosquitoes.rna interference (rnai) has been used as a gene silencing strategy by the introduction of long double stranded rna (dsrna) for the control of pest insects. the aim of the present study was to examine whether the expression of vg gene which is responsible for wing development, can be repressed by chitosan/dsrna based nanoparticles in aedes aegypti. the vestigial gene (vg) was amplified from adult mosquito and cloned in plitmus28i vector. genetically engineered recombinant plasmid was transformed ...201626794313
larvicidal and repellent potential of zingiber nimmonii (j. graham) dalzell (zingiberaceae) essential oil: an eco-friendly tool against malaria, dengue, and lymphatic filariasis mosquito vectors?mosquitoes (diptera: culicidae) are important vectors of terms of public health relevance, especially in tropical and sub-tropical regions. the continuous and indiscriminate use of conventional pesticides for the control of mosquito vectors has resulted in the development of resistance and negative impacts on non-target organisms and the environment. therefore, there is a need for development of effective mosquito control tools. in this study, the larvicidal and repellent activity of zingiber ni ...201626792432
exploiting intimate relationships: controlling mosquito-transmitted disease with wolbachia.mosquito-transmitted diseases impose a growing burden on human health, and current control strategies have proven insufficient to stem the tide. the bacterium wolbachia is a novel and promising form of control for mosquito-transmitted disease. it manipulates host biology, restricts infection with dengue and other pathogens, and alters host reproduction to promote rapid spread in the field. in this review, we examine how the intimate and diverse relationships formed between wolbachia and their mo ...201626776329
calcium influx enhances neuropeptide activation of ecdysteroid hormone production by mosquito ovaries.a critical step in mosquito reproduction is the ingestion of a blood meal from a vertebrate host. in mosquitoes like aedes aegypti, blood feeding stimulates the release of ovary ecdysteroidogenic hormone (oeh) and insulin-like peptide 3 (ilp3). this induces the ovaries to produce ecdysteroid hormone (ecd), which then drives egg maturation. in many immature insects, prothoracicotropic hormone (ptth) stimulates the prothoracic glands to produce ecd that directs molting and metamorphosis. the recep ...201626772671
temporal genetic stability of stegomyia aegypti (= aedes aegypti) populations.the mosquito stegomyia aegypti (= aedes aegypti) (diptera: culicidae) is the primary vector of viruses that cause yellow fever, dengue and chikungunya fever. in the absence of effective vaccines, the reduction of these diseases relies on vector control strategies. the success of these strategies is tightly linked to the population dynamics of target populations. in the present study, 14 collections from st. aegypti populations separated by periods of 1-13 years were analysed to determine their t ...201626744174
detection of aedes aegypti, aedes albopictus, and aedes koreicus in the area of sochi, russia.following the identification of aedes (ae.) aegypti in the sochi area in russia at the beginning of 2000, entomological surveys were conducted during the summers of 2007, 2011, and 2012, leading to the identification of ae. albopictus and ae. koreicus. these findings highlight russia as being the only country in the world health organization european region with a documented presence of both ae. aegypti and ae. albopictus mosquitoes. both mosquito species are found on the coasts of the black sea ...201626741323
bioactivity and laundering resistance of five commercially available, factory-treated permethrin-impregnated fabrics for the prevention of mosquito-borne diseases: the need for a standardized testing and licensing procedure.personal protective measures against hematophagous vectors constitute the first line of defense against arthropod-borne diseases. in this regard, a major advance has been the development of residual insecticides that can be impregnated into clothing. currently, however, information on specific treatment procedures, initial insecticide concentrations, arthropod toxicity, residual activity, and laundering resistance is either fragmentary or non-existent, and no world health organization pesticides ...201626738734
species distribution modelling of aedes aegypti in two dengue-endemic regions of pakistan.statistical tools are effectively used to determine the distribution of mosquitoes and to make ecological inferences about the vector-borne disease dynamics. in this study, we utilised species distribution models to understand spatial patterns of aedes aegypti in two dengue-prevalent regions of pakistan, lahore and swat. species distribution models can potentially indicate the probability of suitability of ae. aegypti once introduced to new regions like swat, where invasion of this species is a ...201626729239
determination of insecticidal effect (lc50 and lc90) of organic fatty acids mixture (c8910+silicone) against aedes aegypti and aedes albopictus (diptera: culicidae).emerging and re-emerging vector-borne diseases such as chikungunya and dengue and associated aedes vectors are expanding their historical ranges; thus, there is a need for the development of novel insecticides for use in vector control programs. the mosquito toxicity of a novel insecticide and repellent consisting of medium-chain carbon fatty acids (c8910) was examined. determination of lc50 and lc90 was made against colony-reared aedes aegypti (l.) and aedes albopictus (skuse) using probit anal ...201626718717
the role of octopamine receptor agonists in the synergistic toxicity of certain insect growth regulators (igrs) in controlling dengue vector aedes aegypti (diptera: culicidae) mosquito.the synergistic action of octopamine receptor agonists (or agonists) on many insecticide classes (e.g., organophosphorus, pyrethroids, and neonicotinoids) on aedes aegypti l. has been reported recently. an investigation of or agonist's effect on insect growth regulators (igrs) was undertaken to provide a better understanding of the mechanism of action. based on the igr bioassay, pyriproxyfen was the most potent igr insecticide tested (ec50=0.0019ng/ml). however, the lethal toxicity results indic ...201626672383
off! clip-on repellent device with metofluthrin tested on aedes aegypti (diptera: culicidae) for mortality at different time intervals and distances.the off! clip-on mosquito-repellent device was tested outdoors against aedes aegypti (l.). a single treatment device was used against batches of caged adult, nonblood fed ae. aegypti at multiple locations 0.3m from treatment center. another set of cages was stationed 0.6m from treatment. a final set of cages was placed 0.9m away. trials ran for durations of 5, 15, 30, and 60 min. initial knockdown and mortality after 24 h was recorded. the devices had effective knockdown and mortality. this was ...201626668103
expression profiles and rnai silencing of inhibitor of apoptosis transcripts in aedes, anopheles, and culex mosquitoes (diptera: culicidae).effective mosquito control is vital to curtail the devastating health effects of many vectored diseases. rna interference (rnai)-mediated control of mosquitoes is an attractive alternative to conventional chemical pesticides. previous studies have suggested that transcripts for inhibitors of apoptosis (iaps) may be good rnai targets. to revisit and extend previous reports, we examined the expression of aedes aegypti (l.) iaps (aaeiaps) 1, 2, 5, 6, 9, and a viral iap-associated factor (viaf) as w ...201626659858
spatial clustering of aedes aegypti (diptera: culicidae) and its impact on entomological surveillance indicators.spatial clustering of aedes aegypti (l.) was assessed in 0.25-km2 sampling plots located in three different neighborhoods in the city of rio de janeiro, brazil monitoring was carried out with two oviposition traps (collecting either. eggs or adult mosquitoes) over a consecutive 80-wk period. taylor power laws (tpl) were used to assess clustering in both adult and egg collections. the clustering level observed in both tpl models was sufficient to increase by two to three times the sampling effort ...201626659607
oviposition behavior in aedes aegypti and aedes albopictus (diptera: culicidae) in response to the presence of heterospecific and conspecific larvae.in mosquitoes, location of suitable sites for oviposition requires a set of visual, tactile, and olfactory cues that influences females before laying their eggs. the ability of gravid females to distinguish among potential oviposition sites that will or will not support the growth, development, and survival of their progeny is critical. aedes aegypti (l.) and aedes albopictus (skuse) share ecological niches, being highly competitive in larval stage. we studied the oviposition behavior of both sp ...201626634825
genetic deviation in geographically close populations of the dengue vector aedes aegypti (diptera: culicidae): influence of environmental barriers in south india.mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. recently, dengue transmission has strongly increased in urban and semiurban areas, becoming a major international public health concern. aedes aegypti (diptera: culicidae) is a primary vector of dengue. shedding light on genetic deviation in a. aegypti populations is of crucial importance to fu ...201626627691
hydrothermal synthesis of titanium dioxide nanoparticles: mosquitocidal potential and anticancer activity on human breast cancer cells (mcf-7).mosquito vectors (diptera: culicidae) are responsible for transmission of serious diseases worldwide. mosquito control is being enhanced in many areas, but there are significant challenges, including increasing resistance to insecticides and lack of alternative, cost-effective, and eco-friendly products. to deal with these crucial issues, recent emphasis has been placed on plant materials with mosquitocidal properties. furthermore, cancers figure among the leading causes of morbidity and mortali ...201626621285
sustained efficacy of the novel topical repellent tt-4302 against mosquitoes and ticks.mosquitoes and ticks are blood-feeding pests of humans and animals that can vector many important aetiological agents of disease. previous research demonstrated that tt-4302 (guardian(®) wilderness) containing 5% geraniol applied to human subjects gave 5-6 h of repellency against mosquitoes (depending on species) and was repellent to ticks in vitro. this study was conducted to obtain an independent third-party evaluation of tt-4302 against stegomyia aegypti (= aedes aegypti) (diptera: culicidae) ...201626614394
spatial and temporal variation in aedes aegypti and aedes albopictus (diptera: culicidae) numbers in the yogyakarta area of java, indonesia, with implications for wolbachia releases.of mosquito vector populations, particularly through wolbachia endosymbionts. the success of these strategies depends on understanding the dynamics of vector populations. in preparation for wolbachia releases around yogyakarta, we have studied aedes populations in five hamlets. adult monitoring with biogent- sentinel (bg-s) traps indicated that hamlet populations had different dynamics across the year; while there was an increase in aedes aegypti (l.) and aedes albopictus (skuse) numbers in the ...201626576934
identification of a major quantitative trait locus determining resistance to the organophosphate temephos in the dengue vector mosquito aedes aegypti.organophosphate insecticides (op) have extensively been used to control mosquitoes, such as the vector aedes aegypti. unfortunately, op resistance has hampered control programs worldwide. we used quantitative trait locus (qtl) mapping to evaluate temephos resistance in two f1 intercross populations derived from crosses between a resistant ae. aegypti strain (recr) and two susceptible strains (moyod and red). a single major effect qtl was identified on chromosome 2 of both segregating populations ...201626576515
characterization and mosquitocidal potential of neem cake-synthesized silver nanoparticles: genotoxicity and impact on predation efficiency of mosquito natural enemies.mosquitoes (diptera: culicidae) serve as important vectors for a wide number of parasites and pathogens of huge medical and veterinary importance. aedes aegypti is a primary dengue vector in tropical and subtropical urban areas. there is an urgent need to develop eco-friendly mosquitocides. in this study, silver nanoparticles (agnp) were biosynthesized using neem cake, a by-product of the neem oil extraction from the seed kernels of azadirachta indica. agnp were characterized using a variety of ...201626573518
the impact of indoor residual spraying of deltamethrin on dengue vector populations in the peruvian amazon.dengue is an important public health problem in the amazon area of peru, resulting in significant morbidity each year. as in other areas of the world, ultra-low volume (ulv) application of insecticides is the main strategy to reduce adult populations of the dengue vector aedes aegypti, despite growing evidence of its limitations as a single control method. this study investigated the efficacy of deltamethrin s.c. applied through indoor residual spraying (irs) of dwellings in reducing a. aegypti ...201626571068
how important is vertical transmission of dengue viruses by mosquitoes (diptera: culicidae)?vertical transmission of dengue viruses by mosquitoes was discovered at the end of the late 1970s and has been suggested to be a means by which these viruses persist. however, it is unclear how widespread it is in nature, and its importance in the epidemiology of this disease is still debated. here, we review the literature on vertical transmission and discuss its role in dengue's epidemiology and control. we conclude that given the number of studies that failed to find evidence of vertical tran ...201626545718
satyrization and satyrization-resistance in competitive displacements of invasive mosquito species.competitive displacements or reductions of resident populations of insects, often effected by a related species, may be caused by a variety of mechanisms. satyrization is a form of mating interference in which males of one species mate with females of another species, significantly decreasing their fitness and not generating hybrids. satyrization has been established to be the probable cause of competitive displacements of resident mosquitoes by invasive species, especially of aedes aegypti by a ...201626542083
plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review.mosquitoes (diptera: culicidae) are a key threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. mosquito young instars are usually targeted with organophosphates, insect growth regulators and microbial control agents. indoors residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have strong negative effects on human health and the environment. newer and safer tools have been recently implemented to enh ...201626541154
nepenthes ampullaria (nepenthaceae) pitchers are unattractive to gravid aedes aegypti and aedes albopictus (diptera: culicidae).nepenthes pitcher plants are colonized by a variety of specialized arthropods. as aedes mosquitoes are container breeders, nepenthes pitchers are a potential candidate oviposition site for vector species, such as aedes aegypti (l.) and aedes albopictus (skuse). however, aedes spp. are not commonly encountered in nepenthes pitchers, and the environment inside the pitchers of some species is lethal to them. one exception is nepenthes ampullaria jack, whose pitchers are known to be colonized by ae. ...201626518035
first report of leptolegnia chapmanii (peronosporomycetes: saprolegniales) affecting mosquitoes in central brazil.numerous isolates of an oomycete 'fungus', leptolegnia chapmanii, are reported from brazil for the first time. this aquatic pathogen was baited with aedes aegypti sentinel larvae from stagnant, temporary bodies of water in selected locations under secondary tropical forest in and near the central brazilian city of goiânia and from more distant sites in the western and northern regions of the state of goiás. isolates were identified based on their morphological and developmental characters, compa ...201627018147
the response of claudin-like transmembrane septate junction proteins to altered environmental ion levels in the larval mosquito aedes aegypti.septate junctions (sjs) occlude the paracellular pathway and function as paracellular diffusion barriers within invertebrate epithelia. however, integral components of sjs and their contribution to barrier properties have received considerably less attention than those of vertebrate occluding junctions. in arthropods, sj proteins have only been identified in drosophila and among these are three integral claudin-like proteins, megatrachea (mega), sinuous (sinu) and kune-kune (kune), as well as a ...201627004691
ecdysis period and rate deviations of dengue mosquito vector, aedes aegypti reared in different artificial water-holding containers.artificial water-holding containers (awhcs) have been well-documented in many aedes aegypti studies for dengue surveillance and developmental research. hence, we investigated the role of different ahwcs on the development and ecdysis period of ae. aegypti dengue vector, a container breeding mosquito.201627004577
mosquito adulticidal activity of the leaf extracts of spondias mombin l. against aedes aegypti l. and isolation of active principles.aedes aegypti is a domestic mosquito and one of the primary vectors for dengue and yellow fever. since, it is a vector of deadly diseases, its control becomes essential. medicinal plants may be an alternative to adulticidal agents since they contain rich source of bioactive compounds. this study was designed to determine the adulticidal activity of spondias mombin leaf methanol crude extract, n-hexane, dichloromethane and ethyl acetate fractions against female adults of ae. aegypti mosquitoes an ...201627004574
plasmodium delichoni n. sp.: description, molecular characterisation and remarks on the exoerythrocytic merogony, persistence, vectors and transmission.malaria parasite plasmodium (novyella) delichoni n. sp. (haemosporida, plasmodiidae) was found in a widespread eurasian songbird, the common house martin delichon urbicum (hirundinidae). it is described based on the morphology of its blood stages and segments of the mitochondrial cytochrome b and apicoplast genes, which can be used for molecular identification of this species. erythrocytic meronts and gametocytes are strictly nucleophilic, and mature gametocytes possess pigment granules of marke ...201627000087
incremental cost of implementing residual insecticide treatment with delthametrine on top of intensive routine aedes aegypti control.information on the cost of implementing residual insecticide treatment (rit) for aedes control is scarce. we evaluated the incremental cost on top of intensive conventional routine activities of the aedes control programme (acp) in the city of santiago de cuba, cuba.201626996279
chemical composition, toxicity and non-target effects of pinus kesiya essential oil: an eco-friendly and novel larvicide against malaria, dengue and lymphatic filariasis mosquito vectors.mosquitoes (diptera: culicidae) are vectors of important parasites and pathogens causing death, poverty and social disability worldwide, with special reference to tropical and subtropical countries. the overuse of synthetic insecticides to control mosquito vectors lead to resistance, adverse environmental effects and high operational costs. therefore, the development of eco-friendly control tools is an important public health challenge. in this study, the mosquito larvicidal activity of pinus ke ...201626995063
composition and biological activities of the essential oil of piper corcovadensis (miq.) c. dc (piperaceae).essential oil from fresh leaves of the shrub piper corcovadensis (miq.) c. dc was obtained in 0.21% (w/w) yield by hydrodistillation in a clevenger type apparatus. thirty-one components, accounting for 96.61% of the leaf oil, were identified by gas chromatography-mass spectrometry. the major constituents of the oil were 1-butyl-3,4-methylenedioxybenzene (30.62%), terpinolene (17.44%), trans -caryophyllene (6.27%), α-pinene (5.92%), δ-cadinene (4.92%), and limonene (4.46%). bioassays against larv ...201626993082
dna barcoding and wing morphometrics to distinguish three aedes vectors in thailand.aedes aegypti (diptera: culicidae) (l.), ae. albopictus (skuse), and ae. scutellaris (walker) are important mosquito vectors of dengue and chikungunya viruses. they are morphologically similar and sympatric in some parts of their distribution; therefore, there is a risk of incorrect morphological identification. any confusion could have a negative impact on epidemiological studies or control strategies. therefore, we explored two modern tools to supplement current morphological identification: d ...201626987285
effect of two commercial herbicides on life history traits of a human disease vector, aedes aegypti, in the laboratory setting.some mosquito species utilize the small niches of water that are abundant in farmland habitats. these niches are susceptible to effects from agricultural pesticides, many of which are applied aerially over large tracts of land. one principal form of weed control in agricultural systems involves the development of herbicide-tolerant crops. the impact of sub-agricultural levels of these herbicides on mosquito survival and life-history traits of resulting adults have not been determined. the aim of ...201626965703
an animal homolog of plant mep/amt transporters promotes ammonia excretion by the anal papillae of the disease vector mosquito aedes aegypti.the transcripts of three putative ammonia (nh3/nh4 (+)) transporters, rhesus-like glycoproteins aerh50-1, aerh50-2 and amt/mep-like aeamt1 were detected in the anal papillae of larval aedes aegypti quantitative pcr studies revealed 12-fold higher transcript levels of aeamt1 in anal papillae relative to aerh50-1, and levels of aerh50-2 were even lower. immunoblotting revealed aeamt1 in anal papillae as a pre-protein with putative monomeric and trimeric forms. aeamt1 was immunolocalized to the bas ...201626944496
aedes aegypti control in brazil. 201626944024
deltamethrin-mediated survival, behavior, and oenocyte morphology of insecticide-susceptible and resistant yellow fever mosquitos (aedes aegypti).insecticide use is the prevailing control tactic for the mosquito aedes aegypti, a vector of several human viruses, which leads to ever-increasing problems of insecticide resistance in populations of this insect pest species. the underlying mechanisms of insecticide resistance may be linked to the metabolism of insecticides by various cells, including oenocytes. oenocytes are ectodermal cells responsible for lipid metabolism and detoxification. the goal of this study was to evaluate the subletha ...201626943998
[dengue, zika and chikungunya].arboviruses are transmitted by arthropods, including those responsible for the current pandemic: alphavirus (chikungunya) and flaviviruses (dengue and zika). its importance increased in the americas over the past 20 years. the main vectors are aedes aegypti and a. albopictus. dengue infection provides long lasting immunity against the specific serotype and temporary to the other three. subsequent infection by another serotype determines more serious disease. there is a registered vaccine for den ...201626942903
repellent and mosquitocidal effects of leaf extracts of clausena anisata against the aedes aegypti mosquito (diptera: culicidae).mosquitoes are rapidly developing resistance to insecticides that millions of people relied on to protect themselves from the diseases they carry, thereby creating a need to develop new insecticides. clausena anisata is used traditionally as an insect repellent by various communities in africa and asia. for this study, the repellency and adulticidal activities of leaf extracts and compounds isolated from this plant species were evaluated against the yellow fever mosquito, aedes aegypti. in the t ...201626924698
structural insights into the aedes aegypti aquaporins and aquaglyceroporins - an in silico study.there has been a fair bit of understanding on the structure-function relationship of aquaporins (aqps) from plants and vertebrates obtained from available x-ray crystallography data. however, there is a lacuna in understanding the structure of aqps from sanguinivorous insects like the mosquito where it plays a crucial role in survival. in this study, we have built homology models for the aedes aegypti aqps, identified key channel lining residues and compared the structure and sequence with ortho ...201626906718
control of mosquito-borne infectious diseases: sex and gene drive.sterile male releases have successfully reduced local populations of the dengue vector, aedes aegypti, but challenges remain in scale and in separating sexes before release. the recent discovery of the first mosquito male determining factor (m factor) will facilitate our understanding of the genetic programs that initiate sexual development in mosquitoes. manipulation of the m factor and possible intermediary factors may result in female-to-male conversion or female killing, enabling efficient s ...201626897660
spatial spread of dengue in a non-endemic tropical city in northern argentina.after more than eighty years dengue reemerged in argentina in 1997. since then, the largest epidemic in terms of geographical extent, magnitude and mortality, was recorded in 2009. in this report we analyzed the den-1 epidemic spread in orán, a mid-size city in a non-endemic tropical area in northern argentina, and its correlation with demographic and socioeconomic factors. cases were diagnosed by elisa between january and june 2009. we applied a space-time and spatial scan statistic under a poi ...201626875764
[new distribution areas of aedes aegypti in peru]. 201627384639
[suggestions for chikungunya control based on a sensitivity analysis of a mathematical model].chikungunya fever seriously affects peoples' health and causes chronic joint pain and even disability. chikungunya is transmitted by the bite of aedes aegypti and aedes albopictus. outbreaks have been reported in throughout the world, including latin america. mathematical modeling studies of these outbreaks have calculated the values of various​ epidemiological parameters. based on them, a mathematical model was prepared to simulate a chikungunya outbreak in a local population, which was transmi ...201627384636
physical influence on larvicidal and pupicidal activity of the silicone-based monomolecular film.although silicone-based monomolecular film (mmf) has been accepted as larvicide in several countries, its mosquito control potential has never been investigated in thailand. laboratory assessment in this study was conducted to determine the mmf efficacy against aedes aegypti. at the recommended dosage (1ml/m(2) of water surface), mortality of pupae (99.17±0.83%) was significantly greater than mortality of old and young larvae (73.33±9.13, 11.67±3.47%; respectively). pupicidal activity was rapidl ...201627443608
anti-dengue efficacy of bioactive andrographolide from andrographis paniculata (lamiales: acanthaceae) against the primary dengue vector aedes aegypti (diptera: culicidae).the current study investigated the toxic effect of the leaf extract compound andrographolide from andrographis paniculata (burm.f) against the dengue vector ae. aegypti. gc-ms analysis revealed that andrographolide was recognized as the major chemical constituent with the prominent peak area compared with other compounds. all isolated toxic compounds were purified and confirmed through rp-hplc against chemical standards. the larvicidal assays established at 25ppm of bioactive compound against th ...201627443607
historical environmental change in africa drives divergence and admixture of aedes aegypti mosquitoes: a precursor to successful worldwide colonization?increasing globalization has promoted the spread of exotic species, including disease vectors. understanding the evolutionary processes involved in such colonizations is both of intrinsic biological interest and important to predict and mitigate future disease risks. the aedes aegypti mosquito is a major vector of dengue, chikungunya and zika, the worldwide spread of which has been facilitated by ae. aegypti's adaption to human-modified environments. understanding the evolutionary processes invo ...201627439067
efficacy of thai neem oil against aedes aegypti (l.) larvae.trees with larvicidal activity may be found in thailand. we conducted this study to evaluate the efficacy and length of efficacy of thai neem (azadirachta siamensis) oil emulsion and an alginate bead of thai neem oil formulation against early fourth stage aedes aegypti larvae using a dipping test. the thai neem oil emulsion had significantly greater larvicidal activity than the alginate bead formulation at 12 to 60 hours post-exposure (p < 0.01). the thai neem oil formulation resulted in 100% mo ...201627405123
evaluation of clove oil, icaridin, and transfluthrin for spatial repellent effects in three tests systems against the aedes aegypti (diptera: culicidae).one essential oil (clove oil), one skin repellent (icaridin), and one insecticide (transfluthrin) were tested for spatial repellent effects against non-blood-fed female aedes aegypti (l.) mosquitoes. the compounds were tested in acetone dilution series using a y-olfactometer, a double cage system, and a double room system. all compounds exhibited spatial repellent effects at certain concentrations. clove oil required relative high dosages to cause high effects (y-olfactometer 6 mg, double cage 6 ...201627651437
antimosquito property of petroselinum crispum (umbellifereae) against the pyrethroid resistant and susceptible strains of aedes aegypti (diptera: culicidae).the increasing and widespread resistance to conventional synthetic insecticides in vector populations has underscored the urgent need to establish alternatives in the mosquito management system. this study was carried out with the aim to investigate the antimosquito property, larvicidal and adulticidal potential, of plant products against both the pyrethroid-susceptible and resistant strains of aedes aegypti. seventeen plant products, including essential oils and ethanolic extracts, were obtaine ...201627638800
artemisia absinthium-borne compounds as novel larvicides: effectiveness against six mosquito vectors and acute toxicity on non-target aquatic organisms.the eco-friendly control of mosquito vectors is a crucial challenge of public health importance. here we evaluated the larvicidal potential of artemisia absinthium essential oil (eo) and its three major chemical constituents against six mosquito vectors: anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. the eo was obtained by leaf hydro-distillation. its chemical composition was analyzed using gas chromatography-mass s ...201627630101
aedes aegypti in rural areas: public health implications. 201627622476
[zika: a defeat in the struggle against aedes aegypti]. 201627622433
biosynthesis of silver nanoparticles from mangrove plant (avicennia marina) extract and their potential mosquito larvicidal property.to identify the larvicidal activities of silver nanoparticles synthesised with avicennia marina leaf extract against the larvae of aedes aegypti and anopheleus stephensi, in vitro larvicidal activities such as lc50 and lc90 were assessed. further, characterisation such as uv and ftir analysis were carried out for the synthesised silver nanoparticles. the lc50 value of the synthesised silver nanoparticles was identified as 4.374 and 7.406 mg/l for an. stephensi and ae. aegypti larvae respectively ...201627605825
raed a 4: a new 67-kda aedes aegypti mosquito salivary allergen for the diagnosis of mosquito allergy.accurate diagnosis of mosquito allergy has been hampered by the laborious task of obtaining mosquito salivary allergens. we have previously studied 3 recombinant (r) aedes aegypti mosquito salivary allergens: raed a 1, raed a 2 and raed a 3. here, we report the expression, purification, identification and evaluation of raed a 4, a 67-kda α-glucosidase.201627603527
[effectiveness of commercial repellents against aedes aegypti (l.) in yucatan, méxico].we assessed the efficacy of commercial repellents available in yucatan against aedes aegypti, vector of dengue, chikungunya and zika.201627599081
comparative proteome analysis reveals that blood and sugar meals induce differential protein expression in aedes aegypti female heads.aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. during human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. here, we report changes in protein expression in the heads of adult female ae. aegypti mosquitoes in response to the ingestion of blood or sugar. proteins extracted from the heads of ae. aegypti fed exclusively on blood (bf) or sugar (sf) we ...201627343150
redeployment of a conserved gene regulatory network during aedes aegypti development.changes in gene regulatory networks (grns) underlie the evolution of morphological novelty and developmental system drift. the fruitfly drosophila melanogaster and the dengue and zika vector mosquito aedes aegypti have substantially similar nervous system morphology. nevertheless, they show significant divergence in a set of genes co-expressed in the midline of the drosophila central nervous system, including the master regulator single minded and downstream genes including short gastrulation, s ...201627341759
susceptibility to temephos and spinosad in aedes aegypti (diptera: culicidae) from puerto rico.we examined the susceptibility to temephos and spinosad (natular ec) of eight aedes aegypti (l.) populations from puerto rico, following who method (who 2005). enzyme activity was measured for alpha- and beta-esterases, multiple function oxidases, glutathione-s-transferases, and insensitive acetylcholinesterase and was tested for correlation with the susceptibility level. the results showed that larval populations from puerto rico obtained during 2014 were found to be susceptible to both larvici ...201627330095
structural characterization and evaluation of mosquito-larvicidal property of silver nanoparticles synthesized from the seaweed, turbinaria ornata (turner) j. agardh 1848.the silver nanoparticles synthesized from turbinaria ornata (to-agnps) showed spherical with crystalline nature (20-32 nm) was evaluated against fourth instar larvae of three mosquitoes. the maximum activity of to-agnps was recorded on aedes aegypti followed by anopheles stephensi and culex quinquefasciatus with the following lethal concentration values (μg/ml): lc50 of 0.738, 1.134, and 1.494; and lc90 of 3.342, 17.982, and 22.475, respectively. the obtained respective values (μg/ml) vis-a-vis ...201627327539
temporal frequency of knockdown resistance mutations, f1534c and v1016g, in aedes aegypti in chiang mai city, thailand and the impact of the mutations on the efficiency of thermal fogging spray with pyrethroids.in thailand, control of dengue outbreaks is currently attained by the use of space sprays, particularly thermal fogging using pyrethroids, with the aim of killing infected aedes mosquito vectors in epidemic areas. however, the principal dengue vector, aedes aegypti, is resistant to pyrethroids conferred mainly by mutations in the voltage-gated sodium channel gene, f1534c and v1016g, termed knockdown resistance (kdr). the objectives of this study were to determine the temporal frequencies of f153 ...201627325294
toxicological assessment of spinosad: implications for integrated control of aedes aegypti using larvicides and larvivorous fish.integration of larvivorous fish and biolarvicides at low concentrations to control of mosquito larvae in field situations may result in a safer and more effective tool. however, the usefulness of integrated approach depends upon survival and ecological fitness of fish employed. thus, the aim of this study was to examine the genotoxic effects of combining different sublethal concentrations of spinosad, a naturally occurring neurotoxic insecticide, with male adult poecilid larvivorous guppy (poeci ...201627294296
effects of preexposure to deet on the downstream blood-feeding behaviors of aedes aegypti (diptera: culicidae) mosquitoes.mosquito behavior is heavily influenced by the chemical molecules in the environment. this knowledge can be used to modify insect behaviors; particularly to reduce vector-host contact as a powerful method for disease prevention. n,n-diethyl-meta-toluamide (deet) is the most widely used insect repellent in the market and an excellent example of a chemical that has been used to modify insect behavior for disease prevention. however, genetic insensitivity and habituation in aedes aegypti (l.) mosqu ...201627288690
somatostatin signaling system as an ancestral mechanism: myoregulatory activity of an allatostatin-c peptide in hydra.the coordination of physiological processes requires precise communication between cells. cellular interactions allow cells to be functionally related, facilitating the maintaining of homeostasis. neuropeptides functioning as intercellular signals are widely distributed in metazoa. it is assumed that neuropeptides were the first intercellular transmitters, appearing early during the evolution. in cnidarians, neuropeptides are mainly involved in neurotransmission, acting directly or indirectly on ...201627288244
chemical composition and biological activity of essential oils from different species of piper from panama.the chemical composition of leaf essential oils from 11 species of piper from panama was analyzed by a combination gc-fid and gc-ms procedures. six of them had sesquiterpene hydrocarbons as major constituents, three were characterized by monoterpene hydrocarbons, one by a diterpene, and one by a phenylpropanoid, dillapiole. the main components identified in each species were: cembratrienol (25.4 %) in piper augustum; β-pinene (26.6 %) in piper corrugatum; α-pinene (19.4 %) in piper curtispicum; ...201627286333
reported distribution of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus in the united states, 1995-2016 (diptera: culicidae).aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) transmit arboviruses that are increasing threats to human health in the americas, particularly dengue, chikungunya, and zika viruses. epidemics of the associated arboviral diseases have been limited to south and central america, mexico, and the caribbean in the western hemisphere, with only minor localized outbreaks in the united states. nevertheless, accurate and up-to-date information for the geographical ranges of ae. aeg ...201627282817
semi-field evaluation of metofluthrin-impregnated nets on host-seeking aedes aegypti and anopheles dirus.the efficacy of a metofluthrin-impregnated net (min) known as the "mushikonazu" on the house entry behavior of female aedes aegypti and anopheles dirus mosquitoes was evaluated using a semi-field 50-m tunnel setup. while the min is labeled for the control of chironomids and moth flies, this study determined the feasibility of using the device, given its current construction and metofluthrin formulation, as a spatial repellent against mosquitoes. sentinel and cone bioassays were used to determine ...201627280351
new candidates for plant-based repellents against aedes aegypti.based on an ethnobotanical study on use for plant species against mosquito bites in the kota tinggi district, johor state, malaysia, 3 plants selected for study, citrus aurantifolia (leaves), citrus grandis (fruit peel), and alpinia galanga (rhizome), were extracted using hydrodistillation to produce essential oils. these essential oils were then formulated as a lotion using a microencapsulation process and then tested for their repellent effect against aedes aegypti. n,n-diethyl-m-toluamide (de ...201627280349
toxicology of potassium channel-directed compounds in mosquitoes.potential targets for new vector control insecticides are nerve and muscle potassium channels. in this study, the activities of known potassium channel blockers (4-aminopyridine, quinidine, and tetraethylammonium) and the insecticide propoxur were compared to three experimental catechols and several other compounds against anopheles gambiae and aedes aegypti mosquitoes. experimental catechol 1 was the most toxic experimental compound in all of the mortality assays conducted, but was at least 100 ...201627262624
evaluation of alternative killing agents for aedes aegypti (diptera: culicidae) in the gravid aedes trap (gat).the gravid aedes trap (gat) uses visual and olfactory cues to attract gravid aedes aegypti (l.) that are then captured when knocked down by a residual pyrethroid surface spray. however, the use of surface sprays can be compromised by poor availability of the spray and pesticide resistance in the target mosquito. we investigated several "alternative" insecticide and insecticide-free killing agents for use in the gat. this included long-lasting insecticide-impregnated nets (llins), vapor-active sy ...201627247350
species composition and habitat characterization of mosquito (diptera: culicidae) larvae in semi-urban areas of dhaka, bangladesh.mosquito larvae are purely aquatic and develop in water bodies, the type of which is more or less specific to each species. therefore, a study was carried out to identify the habitat characters of different mosquito species along with their species composition in semi-urban area of dhaka in bangladesh during the month of may and june 2012. a total of 6088 mosquito larvae belonging to 12 species (aedes aegypti, aedes albopictus, anopheles barbirostris, anopheles peditaeniatus, anopheles vagus, cu ...201627241953
effects of temperature on the life cycle, expansion, and dispersion of aedes aegypti (diptera: culicidae) in three cities in paraiba, brazil.the mosquito aedes aegypti is the primary vector of dengue and is common throughout tropical and subtropical regions. its distribution is modulated by environmental factors, such as temperature. this study aimed to evaluate the influence of temperature on the life cycle and expansion of ae. aegypti populations in the cities of campina grande, joão pessoa, and patos. samples of ae. aegypti were collected in the three cities and raised in the laboratory. we assessed the life cycles of the three ae ...201627232118
the effect of wolbachia on dengue outbreaks when dengue is repeatedly introduced.use of the wolbachia bacterium is a proposed new strategy to reduce dengue transmission, which results in around 390 million individuals infected annually. in places with strong variations in climatic conditions such as temperature and rainfall, dengue epidemics generally occur only at a certain time of the year. where dengue is not endemic, the time of year in which imported cases enter the population plays a crucial role in determining the likelihood of outbreak occurrence. we use a mathematic ...201627217229
yellow fever vaccination status and safety in hemodialysis patients.the adverse effects of yellow fever (yf) vaccine in dialysis patients are not well known. there is concern about the risks and benefits of the vaccine in immunocompromised patients living in endemic areas, particularly given the risk of resurgence of urban yf with the spread of aedes aegypti mosquitoes. the purpose of this study was to assess the coverage and safety of yf vaccine in chronic dialysis patients.201627208638
urban climate versus global climate change-what makes the difference for dengue?the expansion in the geographical distribution of vector-borne diseases is a much emphasized consequence of climate change, as are the consequences of urbanization for diseases that are already endemic, which may be even more important for public health. in this paper, we focus on dengue, the most widespread urban vector-borne disease. largely urban with a tropical/subtropical distribution and vectored by a domesticated mosquito, aedes aegypti, dengue poses a serious public health threat. temper ...201627197685
entomological assessment of yellow fever-epidemic risk indices in benue state, nigeria, 2010-2011.yellow fever (yf) is a vector-borne disease affecting humans and non-human primates in tropical areas. in the past, there have been pockets of yf outbreaks in nigeria that resulted in preventable deaths. surveillance efforts towards avoiding another outbreak have been put in place with the aim of early detection and control. however, risk indices relating to the density of immature yf-mosquito vectors are given little consideration even though it is the first step in curbing a possible outbreak. ...201627189925
functional and immunohistochemical characterization of cceae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors aedes aegypti and ae. albopictus.temephos is a major organophosphate (op) larvicide that has been used extensively for the control of aedes albopictus and aedes aegypti, the major vectors for viral diseases, such as dengue fever, zika and chikungunya. resistance to temephos has been recently detected and associated with the upregulation of carboxylesterases (cces) through gene amplification, in both species. here, we expressed the cceae3a genes which showed the most striking up-regulation in resistant aedes strains, using the b ...201627180726
dsrna-mediated targeting of ribosomal transcripts rps6 and rpl26 induces long-lasting and significant reductions in fecundity of the vector aedes aegypti.ribosomal transcripts produce critical proteins that are involved in most cellular production processes. targeting ribosomal transcripts has produced mortality in mites and ticks but the effect of ribosomal transcript knockdown has not been thoroughly examined in mosquitoes. we examine the effects of triggers targeting four ribosomal proteins (rp) transcripts. although no significant mortality was observed after dsrna microinjection and subsequent blood feeding, significant contrasts were observ ...201627180677
toxicological evaluation of essential oil from the leaves of croton tetradenius (euphorbiaceae) on aedes aegypti and mus musculus.for control of aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of croton tetradenius on ae. aegypti larvae and adults. we also evaluated the acute toxicity in mus musculus. the essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. female mice were used for assessing tox ...201627169864
yellow fever cases in asia: primed for an epidemic.there is currently an emerging outbreak of yellow fever in angola. cases in infected travellers have been reported in a number of other african countries, as well as in china, representing the first ever documented cases of yellow fever in asia. there is a large chinese workforce in angola, many of whom may be unvaccinated, increasing the risk of ongoing importation of yellow fever into asia via busy commercial airline routes. large parts of the region are hyperendemic for the related flavivirus ...201627156836
δ-cadinene, calarene and .δ-4-carene from kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes.mosquitoes (diptera: culicidae) are major vectors of important pathogens and parasites. malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis cause millions of deaths every year. mosquito control is being challenging due to the development of pesticide resistance and negative environmental concerns. in this scenario, plants employed in traditional asian medicine may be alternative sources of newer and effective mosquitocides. in this research, we evaluated th ...201627151483
impact of inter- and intra-specific competition among larvae on larval, adult, and life-table traits of aedes aegypti and aedes albopictus females.few studies have taken a comprehensive approach of measuring the impact of inter- and intra-specific larval competition on adult mosquito traits. in this study, the impact of competition aedes aegypti and a. albopictus was quantified over the entire life of a cohort.competitive treatments affected hatch-to-adult survivorship and development time to adulthood of females for both species, but affected median wing length of females only for a. albopictus. competitive treatments had no significant e ...201627141149
multiple qtl determine dorsal abdominal scale patterns in the mosquito aedes aegypti.the mosquito, aedes aegypti (l.) originated in sub-saharan africa as a dark form sylvan species (a. aegypti formosus). evolution of a. aegypti aegypti type form as a human commensal facilitated its colonization of most semitropical and tropical areas. we investigated the genetic basis for abdominal white scale presence that represents the diagnostic for sylvan a. aegypti formosus (scales absent), from type form (scales present) and a. aegypti queenslandensis form (dense scaling). we performed qu ...201627130203
insights into the evolution of chemoreceptor genes superfamily in tyrophagus putrescentiae (acari: acaridae).all living organisms, including animals, plants, fungi, and bacteria, use the olfactory system to recognize chemicals or pheromone from their environment. insects detect a volatile substance using odorant receptors (ors) or gustatory receptors (grs) and ionotropic receptors (irs). the gene families of the olfactory system in acari are still not clear. in this study, we identified seven ors, one gr, and five irs from the transcriptome of the storage mite, tyrophagus putrescentiae schrank. no olfa ...201627113112
detection and distribution of v1016ikdr mutation in the voltage-gated sodium channel gene in aedes aegypti (diptera: culicidae) populations from sergipe state, northeast brazil.aedes aegypti (l.) resistance to pyrethroids was recorded in brazil few years after its introduction as the adulticide in the national dengue control program campaigns. altered susceptibility to pyrethroids had been reported in the state of sergipe, northeast brazil, through biological assays, even before its use against ae. aegypti in the state. metabolic and target-site resistance mechanisms were also revealed in samples from aracaju, the capital of sergipe. herein, we investigated the presenc ...201627113110
recent changes in the local distribution of aedes aegypti (diptera: culicidae) in south florida, usa.disease transmission is directly tied to the spatial distribution of disease vectors. the distribution of aedes aegypti (l.) in the united states has diminished since the introduction of aedes albopictus (skuse) in the 1980s. however, ae. aegypti persists in some urban areas, particularly in south florida. the pattern of habitat segregation of these two species is well documented, but the consistency of this phenomenon over time is unknown. to examine the dynamics of the local distributions of t ...201627113103
characteristics and efficacy of three commercial handheld thermal foggers with pyrethroid insecticides against three species of mosquitoes.the field study's objectives were to compare the physical characteristics as well as efficacy with multiple insecticides for bonide(®) fog rx insect fogger, black flag(®) propane insect fogger, and burgess(®) outdoor propane insect fogger. evaluations were conducted with 7 machine chemical combinations, 3 depths of spray, and 3 species of laboratory-reared mosquitoes, anopheles quadrimaculatus, culex quinquefasciatus, and aedes aegypti. combinations of these factors were analyzed in conjunction ...201627105215
age-stage, two-sex life table characteristics of aedes albopictus and aedes aegypti in penang island, malaysia.the life table developmental attributes of laboratory colonies of wild strains of aedes albopictus and aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (r0), and mean generation time (t). the total preadult development time was 9.47 days for ae. albopictus and 8.76 days fo ...201627105211
comparative analysis of midgut bacterial communities in three aedine mosquito species from dengue-endemic and non-endemic areas of rajasthan, india.dengue viruses are transmitted to humans through the bites of infected female aedine mosquitoes. differences in the composition and structure of bacterial communities in the midguts of mosquitoes may affect the vector's ability to transmit the disease. to investigate and analyse the role of midgut bacterial communities in viral transmission, midgut bacteria from three species, namely stegomyia aegypti (= aedes aegypti), fredwardsius vittatus (= aedes vittatus) and stegomyia albopicta (= aedes al ...201627094337
profile distribution of juvenile aedes species in an urban area of malaysia.dengue fever (df) is an urban vector-borne disease transmitted by aedes aegypti and aedes albopictus. both species deposit their eggs in favorable breeding sites either in natural or artificial containers. an understanding of their habitat characteristics is crucial in curbing df outbreaks201627076510
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