Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| development of monoclonal antibodies which specifically recognize entamoeba histolytica in preserved stool samples. | we report the generation of monoclonal antibodies against a recombinant 170-kda subunit of the gal or galnac lectin of entamoeba histolytica that specifically recognize e. histolytica but not entamoeba dispar in preserved stool samples. these antibodies do not cross-react with other bowel protozoa, including entamoeba coli, giardia lamblia, and dientamoeba fragilis. | 2001 | 11158133 |
| the unusual transhydrogenase of entamoeba histolytica. | we have expressed and purified a protein fragment from entamoeba histolytica. it catalyses transhydrogenation between analogues of nad(h) and nadp(h). the characteristics of this reaction resemble those of the reaction catalysed by a complex of the nad(h)- and nadp(h)-binding subunits of proton-translocating transhydrogenases from bacteria and mammals. it is concluded that the complete en. histolytica protein, which, along with similar proteins from other protozoan parasites, has an unusual subu ... | 2001 | 11163794 |
| granulysin: a novel antimicrobial. | granulysin is a novel lytic molecule produced by human cytolytic t-lymphocytes (ctls) and natural killer (nk) cells. it is active against a broad range of microbes, including gram-positive and -negative bacteria, parasites and mycobacterium tuberculosis. it is functionally related to other antibacterial peptides, like defensins and magainins, but is structurally distinct. it has structural similarity to porcine nk-lysin and to amoebapores made by entamoeba histolytica. synthetic peptides derived ... | 2001 | 11178344 |
| a review of plant species assessed in vitro for antiamoebic activity or both antiamoebic and antiplasmodial properties. | the resurgence of the protozoal diseases amoebiasis and malaria has been known to occur, from time to time, in endemic and epidemic proportions all over the world. furthermore, the import of these individual pathogens to other areas from tropical regions encourages these protozoal diseases to occur on a global scale with considerable associated mortality and morbidity. from time immemorial, the cure of these diseases has been attempted with the use of traditional plant products, derived from suc ... | 2001 | 11180515 |
| high prevalence of infection with entamoeba dispar, but not e. histolytica, in captive macaques. | a total of 268 nonhuman primates (20 species) kept in the primate research institute, kyoto university, japan, were surveyed for intestinal amebas. total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: entamoeba histolytica/e. dispar, 53%; e. coli, 34%; e. hartmanni, 34%; iodamoeba buetschlii, 25%; endolimax nana, 8%; and e. chattoni, 3%. positive rates were higher in old world monkeys and lower in new world monkeys. all the 1 ... | 2001 | 11199843 |
| entamoeba histolytica: production of nitric oxide and in situ activity of nadph diaphorase in amebic liver abscess of hamsters. | entamoeba histolytica trophozoites were inoculated into the liver of hamsters and serum nitrate/nitrite levels [expressed as nitric oxide (no) production] were determined at different times during amebic liver abscess (ala) development. we also tested the effects of no synthase (nos) inhibitors such as n(g)-nitro-l-arginine methyl ester (l-name), aminoguanidine, and dexamethasone during ala production. since nos activity has been correlated with expression of reduced nicotinamide adenine dinucle ... | 2001 | 11199849 |
| protection of hamsters from amebic liver abscess formation by immunization with the 150- and 170-kda surface antigens of entamoeba histolytica. | a monoclonal antibody, eh3015, prevents in vitro adherence of entamoeba histolytica trophozoites to mammalian cells and inhibits amebic liver abscess formation in hamsters. by immunoaffinity chromatography with the monoclonal antibody, purified e. histolytica antigens with molecular masses of 150 and 170 kda under non-reduced conditions were obtained. hamsters were immunized with these antigens (group i) or with fractions further purified by polyacrylamide gel electrophoresis (group ii). pooled ... | 2001 | 11206109 |
| synthesis and antiparasitic activity of 2-(trifluoromethyl)-benzimidazole derivatives. | 2-(trifluoromethyl)benzimidazole derivatives substituted at the 1-, 5-, and 6-positions have been synthesized and in vitro tested against the protozoa giardia lamblia, entamnoeha histolytica. and the helminth trichinella spiralis. results indicate that all the compounds tested are more active as antiprotozoal agents than albendazole and metronidazole. one compound (20) was as active as albendazole against t. spiralis. these compounds were also tested for their effect on tubulin polymerization an ... | 2001 | 11206455 |
| overexpression of cysteine proteinase 2 in entamoeba histolytica or entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils. | to study the role of cysteine proteinases in the pathogenicity of entamoeba histolytica, we have attempted to overexpress the three main cysteine proteinases (ehcp1, ehcp2, ehcp5) of this parasite in trophozoites of e. histolytica as well as in non-pathogenic entamoeba dispar by episomal transfection. although each of the corresponding coding sequences were cloned in identical expression plasmids, we were unable to overexpress ehcp1 and ehcp5, respectively, but could substantially induce express ... | 2001 | 11207616 |
| a novel anti-inflammatory oligopeptide produced by entamoeba histolytica. | the monocyte locomotion inhibitory factor (mlif), a heat-stable oligopeptide found in the supernatant fluid of entamoeba histolytica axenic cultures was isolated by ultra-filtration, gel-sieve chromatography and high powered liquid chromatography (hplc), and its primary structure (met-gln-cys-asn-ser) established by edman sequencing and mass-spectrometry (ms). a synthetic peptide had the same selective anti-inflammatory features as the native material in comparable concentrations: in vitro inhib ... | 2001 | 11223127 |
| the cell cycle of entamoeba invadens during vegetative growth and differentiation. | the cell division cycle of entamoeba invadens was studied during vegetative growth of trophozoites and during their differentiation into cysts. during vegetative growth of trophozoites, it was observed that dna synthesis typically continued after one genome content had been duplicated. during encystation, dna synthesis was arrested after 4n genome content had been synthesised. using multi-parameter flow cytometry, the light scattering properties of cysts and trophozoites were studied. the cytopl ... | 2001 | 11223134 |
| [diagnostic image (8). intestinal amoebiasis]. | 2001 | 11225255 | |
| a plasma membrane-type ca(2+)-atpase co-localizes with a vacuolar h(+)-pyrophosphatase to acidocalcisomes of toxoplasma gondii. | ca(2+)-atpases are likely to play critical roles in the biochemistry of toxoplasma gondii, since these protozoa are obligate intracellular parasites and the ca(2+) concentration in their intracellular location is three orders of magnitude lower than in the extracellular medium. here, we report the cloning and sequencing of a gene encoding a plasma membrane-type ca(2+)-atpase (pmca) of t.gondii (tga1). the predicted protein (tga1) exhibits 32-36% identity to vacuolar ca(2+)-atpases of trypanosoma ... | 2001 | 11226155 |
| amebic infection in the human colon induces cyclooxygenase-2. | we sought to determine if infection of the colon with entamoeba histolytica induces the expression of cyclooxygenase-2 and, if it does, to determine the contribution of prostaglandins produced through cyclooxygenase-2 to the host response to amebic infection. human fetal intestinal xenografts were implanted subcutaneously in mice with severe combined immunodeficiency and allowed to grow; the xenografts were then infected with e. histolytica trophozoites. infection with e. histolytica resulted in ... | 2001 | 11292761 |
| amoebicidal and giardicidal compounds from the leaves of zanthoxylum liebmannianun. | the crude ethanol extract from the leaves of zanthoxylum liebmannianum exhibited inhibitory effect on the reproduction of trophozoites of entamoeba histolytica (ic(50)=3.48 microg/ml) and giardia lamblia (ic(50)=58.00 microg/ml). from this extract, asarinin, hyperin, beta-sitosterol, and beta-sitosterol glucoside were isolated. among them, asarinin was the most active with ic(50) values of 19.86 microg/ml for e. histolytica and 35.45 microg/ml for g. lamblia. the remaining compounds showed moder ... | 2001 | 11295310 |
| effects of miltefosine and other alkylphosphocholines on human intestinal parasite entamoeba histolytica. | the protozoan parasite entamoeba histolytica is the cause of amoebic dysentery and liver abscess. it is therefore responsible for significant morbidity and mortality in a number of countries. infections with e. histolytica are treated with nitroimidazoles, primarily with metronidazole. at this time, there is a lack of useful alternative classes of substances for the treatment of invasive amoebiasis. alkylphosphocholines (alkyl-pcs) such as hexadecyl-pc (miltefosine) were originally developed as ... | 2001 | 11302818 |
| effect of jasplakinolide on the growth, encystation, and actin cytoskeleton of entamoeba histolytica and entamoeba invadens. | the effect of jasplakinolide. an actin-polymerizing and filament-stabilizing drug, on the growth, encystation, and actin cytoskeleton of entamoeba histolytica and entamoeba invadens was examined. jasplakinolide inhibited the growth of e. histolytica strain hm-1:imss and e. invadens strain ip-1 in a concentration-dependent manner, the latter being more resistant to the drug. the inhibitory effect of jasplakinolide on the growth of e. histolytica trophozoites was reversed by removal of the drug af ... | 2001 | 11318572 |
| selective 'unlabeling' of amino acids in fractionally 13c labeled proteins: an approach for stereospecific nmr assignments of ch3 groups in val and leu residues. | a novel methodology for stereospecific nmr assignments of methyl (ch3) groups of val and leu residues in fractionally 13c-labeled proteins is presented. the approach is based on selective 'unlabeling' of specific amino acids in proteins while fractionally 13c-labeling the rest. a 2d [13c-1h] hsqc spectrum recorded on such a sample is devoid of peaks belonging to the 'unlabeled' amino acid residues. such spectral simplification aids in unambiguous stereospecific assignment of diastereotopic ch3 g ... | 2001 | 11330814 |
| microbes and microbial toxins: paradigms for microbial-mucosal interactions. vi. entamoeba histolytica: parasite-host interactions. | the protozoan intestinal parasite entamoeba histolytica remains a significant cause of morbidity and mortality worldwide. e. histolytica causes two major clinical syndromes, amebic colitis and amebic liver abscess. recent advances in the development of in vitro and in vivo models of disease, new genetic approaches, the identification of key e. histolytica virulence factors, and the recognition of crucial elements of the host response to infection have led to significant insights into the pathoge ... | 2001 | 11352795 |
| intestinal amebiasis: a diagnosis not to be missed. | entamoeba histolytica is a well-recognized cause of infectious colitis and disseminated amebic abscesses. most prevalent in the tropics and subtropics, e. histolytica infections may also occur in the developed world. we describe a case of a north american traveler with intestinal amebiasis, a diagnosis first made by colonic biopsy. we review the available diagnostic tools and the role of the surgical pathologist in the detection of this infection. | 2001 | 11358014 |
| amoeboma of ascending colon with multiple amoebic liver abscesses. | a case of amoeboma of the ascending colon with multiple amoebic liver abscess is being presented; which was mistakenly diagnosed as carcinoma of ascending colon with multiple secondaries in liver. awareness of this previously unreported association is important because it adds to our knowledge of the spectrum of intestinal amoebiasis. | 2001 | 11361280 |
| amebiasis and mucosal iga antibody against the entamoeba histolytica adherence lectin in bangladeshi children. | amebiasis is the third leading parasitic cause of death worldwide, and it is not known whether immunity is acquired from a previous infection. an investigation was done to determine whether protection from intestinal infection correlated with mucosal or systemic antibody responses to the entamoeba histolytica galnac adherence lectin. e. histolytica colonization was present in 0% (0/64) of children with and 13.4% (33/246) of children without stool iga anti-galnac lectin antibodies (p= .001). chil ... | 2001 | 11372032 |
| antiprotozoal activity of the constituents of conyza filaginoides. | bioassay-guided fractionation of the antiprotozoal extract of conyza filaginoides led to the isolation of three new flavonol caffeoyl glycosides, namely, kaempferol 3-o-(6' '-o-e-caffeoyl)-beta-d-galactopyranoside (1), isorhamnetin 3-o-(6' '-o-e-caffeoyl)-beta-d-galactopyranoside (2), and quercetin 3-o-(6' '-o-e-caffeoyl)-beta-d-glucopyranoside (3). in addition, seven known compounds, erythrodiol (4), beta-caryophyllene-4,5-alpha-oxide (5), astragalin (6), isoquercitrin (7), nicotiflorin (8), na ... | 2001 | 11374974 |
| evaluation of genetic damage induced by a nitroimidazole derivative in human lymphocytes: tinidazole (tnz). | one of the useful drugs in the treatment against infestations caused by trichomonas vaginalis, entamoeba histolytica and giardia lamblia is tinidazole (tnz) 1-[2-(ethylsulfonyl) ethyl]-2-methyl-5-nitroimidazole) (gilman r.h., marquis g.s., miranda e., vestegui m., martinez h., 1988. rapid reinfection by giardia lamblia after treatment in a hyperendemic third world community. lancet i, 343-345). we decided to evaluate the potential genetic damage induced by tnz using different biological biomarke ... | 2001 | 11377093 |
| pathophysiology of amoebiasis. | few organisms are more aptly named than entamoeba histolytica, an intestinal protozoan parasite that can lyse and destroy human tissue. within the past four years, new models of e. histolytica infection have begun to illuminate how amoebic trophozoites cause intestinal disease and liver abscess, and have expanded our understanding of the remarkable killing ability of this parasite. here, i summarize recent work on the interactions between e. histolytica and human intestine, and between e. histol ... | 2001 | 11378035 |
| differential gene expression by iron-limitation in entamoeba histolytica. | 2001 | 11378206 | |
| effect of an electric field on the motility of entamoeba histolytica examined by multiple-beam interference microscopy. | a recently developed multiple-beam interference microscopic technique has been used to visualize submicroscopic structures of entamoeba histolytica and their movements in applied external electric fields. the movements were videorecorded and it was found that at low current (120 microa) pseudopods are filled with hyaline ectoplasm. at slightly higher current (about 150 microa), the amoeba stops extending the pseudopods and loosens its attachment to the surface. at higher currents (200 microa), i ... | 2001 | 11384161 |
| prevalence of viral, bacterial and parasitic enteropathogens among young children with acute diarrhoea in jeddah, saudi arabia. | the prevalence of viral, bacterial and parasitic pathogens among children of jeddah, saudi arabia, was investigated. during december 1995-october 1996, 576 faecal samples were collected from children (0-5 year(s) old) suffering from acute diarrhoea and attending hospitals and outpatient clinics in jeddah. one or more enteropathogen(s) were identified in 45.6% of the stool specimens. mixed infections were detected in 12.2% of the diarrhoeal cases. rotavirus was detected in 34.6% of the specimens ... | 2001 | 11394180 |
| [training for diagnosis of intestinal parasitic diseases in the national laboratory system of cuba]. | a national training project in the diagnosis of intestinal parasites was conducted in 1997. an initial national course was followed respectively by courses in the central, eastern, and western provinces. our results showed that cryptosporidium parvum, cyclospora cayetanensis, and leukocytes showed a significantly lower percentage of errors after the training than before (p < 0.01). the same occurred with entamoeba histolytica/e. dispar and chilomastix mesnilii (p < 0.05). among the helminths, ta ... | 2001 | 11395809 |
| disseminated acanthamoebiasis presenting as lobular panniculitis with necrotizing vasculitis in a patient with aids. | disseminated acanthamoebiasis is a rare entity, almost exclusively occurring in the immunocompromised host. | 2001 | 11401678 |
| inhibition of encystation of entamoeba invadens by wortmannin. | using an axenic encystation system in vitro, we examined the effect of wortmannin, a potent inhibitor of phosphatidylinositol 3-kinase (pi 3-kinase), which is a signaling molecule responsible for numerous cellular responses, on the encystation of entamoeba invadens. wortmannin inhibited both encystation and growth of e. invadens strain ip-1 in a dose-dependent manner, the former being more resistant to the drug than the latter. there was little decrease in the number of trophozoites after 3 days ... | 2001 | 11403379 |
| amebic liver abscess and human immunodeficiency virus infection: a report of three cases. | invasive amebiasis rarely occurs in homosexual men and human immunodeficiency virus (hiv)-infected individuals and has not been regarded as a beacon for concomitant hiv infection. we encountered a bisexual man with a protracted course of amebic liver abscess and amebic colitis. in the presence of fever, generalized lymphadenopathy, and elevated serum aminotransferase levels, hiv infection was suspected and then confirmed by a de novo seroconversion of hiv antibody. subsequently, we noted two con ... | 2001 | 11418795 |
| modifications of kohn's chlorazol black e staining and wheatley's trichrome staining for temporary wet mount and permanent preparation of entamoeba histolytica. | preparation of stained smears of entamoeba histolytica has several drawbacks. we therefore tried to simplify the staining procedures by modifing kohn's chlorazol black e staining and wheatley's trichrome staining techniques. trophozoites and cysts of axenically cultured e. histolytica and entamoeba invadens, respectively, and trophozoites and cysts of e. histolytica in stools of patients were used. karyosomes and peripheral chromatin of nuclei and chromatoid bodies became distinctly visible afte ... | 2001 | 11426739 |
| a political asylum seeker with an amoebic empyema in birmingham. | 2001 | 11436450 | |
| purification and identification of major soluble 40-kda antigenic protein from entamoeba histolytica: its application for serodiagnosis of asymptomatic amebiasis. | one of the major soluble antigenic proteins of entamoeba histolytica was purified to homogeneity and identified on a molecular basis. its recombinant protein was expressed in escherichia coli as a fusion protein with shistosoma japonicum glutathione s-transferase. apparent molecular weight of the purified antigenic protein was estimated to be 40-kda and molecular-based analysis indicated that the purified protein was nadp+-dependent alcohol dehydrogenase (ehadh1). the application of the purified ... | 2001 | 11438429 |
| molecular cloning and characterization of peroxiredoxin from toxoplasma gondii. | a cdna of 1.1 kb comprising the gene encoding the peroxiredoxin of toxoplasma gondii (tgprx) has been cloned. the open reading frame of 591 bp was translated into a protein of 196 amino acids with a molecular mass of 25 kda. conserved 2 cysteine domains of phe-val-cys-pro and glu-val-cys-pro indicated tgprx belonged to 2-cys prx families. tgprx showed the highest homology with that of arabidopsis thaliana by 53.9% followed by entamoeba histolytica with 39.5% by the amino acid sequence alignment. ... | 2001 | 11441500 |
| [travelers' diarrhea]. | diarrhea is the most common health problem of travelers to tropical destinations, affecting up to over 50%, with however considerable regional and seasonal variation. orally acquired bacterial pathogens, particularly enterotoxigenic escherichia coli, are the most frequent etiology of travelers' diarrhea occurring during the first three weeks of travel. protozoal infections, e.g. giardia and entamoeba histolytica, are more often the cause of diarrhea and prolonged problems of intestinal motility ... | 2001 | 11441697 |
| treatment of diarrhea caused by giardia intestinalis and entamoeba histolytica or e. dispar: a randomized, double-blind, placebo-controlled study of nitazoxanide. | a prospective randomized, double-blind, placebo-controlled study was conducted to assess the efficacy and safety of nitazoxanide in the treatment of diarrhea caused by giardia intestinalis or entamoeba histolytica and/or e. dispar in 89 adults and adolescents, 22 of whom were diagnosed with g. intestinalis, 53 with e. histolytica and/or e. dispar, and 14 with both g. intestinalis and e. histolytica and/or e. dispar. the study medication was administered as 1 nitazoxanide 500-mg tablet or a match ... | 2001 | 11443569 |
| amoebic liver abscess in pregnancy. | we describe the case of an amoebic liver abscess (ala) presenting in the third trimester of pregnancy which raised both diagnostic and treatment dilemmas as well as being associated with preterm labour. amoebic liver abscess is caused by the protozoan organism entamoeba histolytica which is endemic in many parts of the developing world. invasion of the colonic mucosa results in the clinical syndrome of amoebic dysentery and in some cases dissemination to the liver or other organs occurs resultin ... | 2001 | 11453283 |
| multiplex polymerase chain reaction amplification and differentiation of entamoeba histolytica and entamoeba dispar dna from stool samples. | due to the clinical importance of differentiating the two species of the entamoeba histolytica/entamoeba dispar complex, we developed a multiplex polymerase chain reaction (pcr) method that overcomes time-consuming and laborious procedures. we report here a dna extraction protocol using non-fixed stool samples that avoid long lysis-incubation periods through the combined use of zirconium beads and a lysis-supporting buffer. we characterized 49 of 52 stool specimens from cuban patients with amoeb ... | 2001 | 11463120 |
| characterization of a retrotransposon-like element from entamoeba histolytica. | the protozoan parasite entamoeba histolytica is the causative agent of amoebiasis. the genome organization of this organism is not well understood. we had earlier reported the presence of a multicopy sequence, hmc, in e. histolytica. subsequent analysis showed that hmc is a member of a retrotransposon family that we have named the e. histolytica retrotransposon-like element (ehrle). four other members of this family have been characterized. the ehrle family is distributed across all chromosomes ... | 2001 | 11463465 |
| recombinational loss of a ribosomal dna unit from the circular episome of entamoeba histolytica hm-1:imss. | 2001 | 11463474 | |
| entamoeba histolytica: monoclonal antibody against the beta1 integrin-like molecule (140 kda) inhibits cell adhesion to extracellular matrix components. | we describe a monoclonal antibody (3c10) against the beta1 integrin-like molecule which immunoprecipitates two polypeptides of 140 and 155 kda from detergent-soluble extract of entamoeba histolytica. the 140-kda polypeptide has been described as a beta subunit of the amoebic fibronectin receptor as it is recognized by an anti-integrin beta1 (human) monoclonal antibody in immunoblot assay. the receptor molecules were localized with the 3c10 monoclonal antibody in intracellular and surface membran ... | 2001 | 11465991 |
| protective immunity to amebiasis: new insights and new challenges. | amebiasis, infection with the protozoan parasite entamoeba histolytica, is the third leading cause of death from parasitic diseases. despite its importance, we know little about protective immunity to amebiasis. now, studies from a cohort of children in bangladesh have provided some critical insights into immunity to intestinal amebiasis. children with mucosal iga antibodies to the amebic adherence lectin were found to be resistant to reinfection with e. histolytica. however, immunity was short- ... | 2001 | 11471109 |
| detection of giardia lamblia and entamoeba histolytica in stool samples by two enzyme immunoassays. | two commercially produced enzyme immunoassays (eias) to detect antigens of giardia lamblia and entamoeba histolytica in stool specimens were evaluated. a total of 276 stool specimens were collected from patients who presented with various medical complaints in the outpatient clinic of the department of infectious diseases and tropical medicine, university of munich. every specimen was examined by conventional microscopy and tested by both eia kits. when microscopy was used as the reference stand ... | 2001 | 11476438 |
| evaluation of laboratory techniques for differentiation between entamoeba histolytica and entamoeba dispar. | incorporation of clinical, epidemiological and serological parameters to the studies of entamoeba histolytica resulted in the conclusion that species complex comprising two morphologically identical amoeba were implicated with the disease. the two organisms are e. histolytica and e. dispar. the former is a pathogen and responsible for invasive amoebiasis while the later is a gut commensal. clearly accurate diagnostic tools are required to distinguish the two species. the use of a stool elisa has ... | 2001 | 11478433 |
| experimental amoebic liver abcess produced by oral administration of entamoeba histolytica cysts. | to determine the possibility of amoebic invasion and liver-abscess formation swiss albino mice were infected orally with e. histolytica cysts isolated from human stools. parasitological and histopathological changes in mice colon and liver tissues were sequentially followed. three weeks postinfection (p.i) 5% of immunocompetent and all cortisonized immunosuppressed mice passed the parasite in their stools. only 70% of the latter group of mice sacrificed at that time developed invasive intestinal ... | 2001 | 11478441 |
| liver function tests during amoebic liver abscess formation in indomethacin-treated hamsters. | establishment of entamoeba histolytica infection is facilitated through macrophage effector disruption by a prostaglandin e2 (pge2)-mediated mechanism. infection severity may be measured by weight of abscess formed. indomethacin (indo) treatment of infected hamsters reduced abscess weight by 30% at 7 days post-infection presumably by inhibition of pge2. to explain reductions in abscess development by indo treatment, we determined liver functionality in indo-treated or untreated animals, either h ... | 2001 | 11479899 |
| dysentery in patients infected with human immunodeficiency virus in zimbabwe: an emerging role for schistosoma mansoni and escherichia coli o157? | information on bloody diarrhoea in hiv-positives is scarce. a prospective study was therefore performed, in zimbabwe, to determine and compare the pathogens associated with bloody diarrhoea in 25 antiretroviral-naïve hiv-infected patients and 15 non-hiv-infected patients. stool cultures and colonic biopsies were performed. shigella was isolated from 18 (45%) of the subjects, schistosoma mansoni from eight (16%), escherichia coli h7:o157 from three (8%) and campylobacter jejunii from two (5%). th ... | 2001 | 11487372 |
| massive amebic liver abscess--an unusual presentation. | a 40-year-old woman presented with a huge lump in the right half of the abdomen and irregular menses. ultrasonography showed a cystic lump with septations, extending from the upper abdomen to the pelvis; the right ovary was not seen. on exploration, there was a large cyst arising from the right lobe of the liver; the aspirate was bilious. since the cyst wall was adherent to retroperitoneal structures, complete excision was not possible. a roux-en-y loop of jejunum was anastomosed to the cyst wal ... | 2001 | 11497177 |
| intermediate subunit of the gal/galnac lectin of entamoeba histolytica is a member of a gene family containing multiple cxxc sequence motifs. | killing by entamoeba histolytica requires parasite adherence to host galactose- and n-acetyl-d-galactosamine (gal/galnac)-containing cell surface receptors. a 260-kda heterodimeric e. histolytica gal/galnac lectin composed of heavy (hgl) and light (lgl) subunits has been previously described. here we present the cloning and characterization of igl, a 150-kda intermediate subunit of the gal/galnac lectin. igl, hgl, and lgl colocalized on the surface membrane of trophozoites. two unlinked copies o ... | 2001 | 11500468 |
| enteropathogens associated with diarrheal disease in infants of poor urban areas of porto velho, rondônia: a preliminary study. | one hundred and thirty cases of diarrhea and 43 age-matched controls, 0 to 5 years old, were studied in a pediatric outpatient unit from a poor peri urban area of porto velho, rondônia. eighty percent of diarrheal cases were observed in the groups under 2 years of age. rotavirus (19.2%) was the most frequent enteropathogen associated with diarrhea, followed by shigella flexneri (6.15%) and s. sonnei (1.5%) and salmonella sp. (6.9%). four cases of e. coli enterotoxigenic infections (3.1%), e. col ... | 2001 | 11500758 |
| serodiagnosis of amoebiasis using a recombinant protein fragment of the 29 kda surface antigen of entamoeba histolytica. | to develop an improved serodiagnostic test for amoebiasis, we performed a detailed analysis of the immunodominant epitopes of the 29 kda surface antigen and evaluated its sensitivity and specificity. enzyme-linked immunosorbent assay (elisa) based on the fragment containing the immunodominant epitope was evaluated further and compared with full-length recombinant 29 kda protein. specificity and sensitivity of the two elisas were assessed using 55 human sera of parasitic protozoa infection cases ... | 2000 | 11428340 |
| current problems of amebiasis in japan and recent advances in amebiasis research. | amebiasis is epidemic in two major populations in japan: male homosexuals and institutionalized people. currently available diagnostic, chemotherapeutic, and prophylactic measures and their problems are discussed. recent advances in basic research on amebiasis are also described with new findings of unique metabolisms and intracellular structures and organization. discoveries and analyses of the unique features presented by this parasitic protist help in our elucidation of the pathogenic mechani ... | 2000 | 11227020 |
| secondary infections of aids autopsy cases in japan with special emphasis on mycobacterium avium-intracellulare complex infection. | in order to study the frequency of secondary infections of aids autopsy cases in japan, especially the frequency of mycobacterium aviumintracellulare complex (mac) infection, retrospective autopsy study was conducted between 1986 and 1997 at the affiliated hospital of institute of medical sciences, university of tokyo. secondary infections of various organs from 43 aids autopsy cases were examined using histopathology, genetic diagnosis of tuberculosis, ziehl-neelsen stain for acid-fast bacilli ... | 2000 | 11211316 |
| actin induction during pma and camp-dependent signal pathway activation in entamoeba histolytica trophozoites. | activation of pkc or camp-dependent signalling pathways in entamoeba histolytica triggers the phosphorylation of proteins involved in actin rearrangements necessary for adhesion and locomotion. analogous motifs to sre and cre sequences--known to respond to pma and camp--were identified within the 5' regulatory region (5'rr) of one of the parasite actin genes. these sequences could be involved in the actin transcriptional upregulation reported during signalling. to test this hypothesis, a plasmid ... | 2000 | 11207595 |
| caspase 3-dependent killing of host cells by the parasite entamoeba histolytica. | the parasite entamoeba histolytica is named for its ability to lyse host tissues. to determine the factors responsible, we have initiated an examination of the contribution of parasite virulence factors and host caspases to cellular destruction by the parasite. amoebic colitis in c3h/hej mice was associated with extensive host apoptosis at sites of e. histolytica invasion. in vitro studies of e. histolytica-jurkat t-cell interactions demonstrated that apoptosis required contact via the amoebic g ... | 2000 | 11207613 |
| ruptured liver abscess with fulminant amoebic colitis: case report with review. | amoebic liver abscess is the commonest extra intestinal manifestation of amoebiasis. intraperitoneal rupture of liver abscess and fulminant necrotizing amoebic colitis are rare occurrences which complicate a severe form of invasive disease caused by entamoeba histolytica. these complications are associated with a high morbidity and mortality. synchronous pathological lesions in colon and liver are rare. still rare is the occurrence of complicated colonic and hepatic invasive amoebiasis presentin ... | 2000 | 11194588 |
| partial purification and characterization of dolichol phosphate mannose synthase from entamoeba histolytica. | dolichol phosphate mannose synthase, an essential enzyme in glycoprotein biosynthesis, was partially purified from e.histolytica by hydrophobic interaction and affinity chromatography with octyl sepharose cl-4b and affi-gel 501, respectively. reducing agents, particularly dithiothreitol, positively influenced enzyme activity and stability, indicating a role of sulfhydryl groups on the transferase function. activity did not depend on phospholipids; however, it was significantly stimulated by phos ... | 2000 | 11159922 |
| trichomonas vaginalis: chromatin and mitotic spindle during mitosis. | the mitotic phases and the changes that the chromatin and mitotic microtubules undergo during mitosis in the sexually transmitted parasite trichomonas vaginalis are described. parasites arrested in the gap 2 phase of the cell cycle by nutrient starvation were induced to mitosis by addition of fresh whole medium. [(3)h] thymidine labeling of trichomonad parasites for 24 h showed that parasites have at least four synchronic duplications after mitosis induction. fixed or live and acridine orange (a ... | 2000 | 11162363 |
| molecular cloning and cellular localization of an unusual bipartite entamoeba histolytica polypeptide with similarity to actin binding proteins. | 2000 | 11163453 | |
| recent developments in amoebiasis:the gal/galnac lectins of entamoeba histolytica and entamoeba dispar. | amoebiasis is responsible for 50000-100000 deaths annually. invasive amoebic disease begins with the attachment of entamoeba histolytica trophozoites to colonic mucin, a process mediated by the amoebic gal/galnac lectin. the non-pathogenic counterpart, e. dispar, is morphologically identical but genetically distinct. investigations comparing the gal/galnac lectin from these two organisms are under way. | 2000 | 11137050 |
| [a case of aids complicated with liver tuberculosis]. | we experienced a double infection of tuberculosis and amebiasis of the liver. a 28 year old male with aids was admitted to our hospital because of severe diarrhea and liver abscess by entamoeba histolytica. in spite of improvement of the diarrhea and liver abscess by the therapy against e. historicica, serum levels of gamma-gtp and alp remained high and hepatosplenomegaly gradually increased. a liver biopsy was performed. pathology showed a granulomatous lesion with langhans' giant cells. from t ... | 2000 | 11140084 |
| experimental amebiasis: immunohistochemical study of immune cell populations. | distribution of immune cell populations was studied in a c3h/hej mouse model of intestinal amebiasis from 5 to 60 days post-inoculation with entamoeba histolytica, using immunoperoxidase techniques. at various time intervals, the ceca from mice were fixed in 10% formalin, dehydrated, embedded and sectioned at 5 microm. sections were incubated with conjugated peroxidase-labelled antibodies to mouse iga, igm, and igg. color was developed with 3, 3'-diaminobenzidine tetrahydrochloride (dab)/h2o2 so ... | 2000 | 11140454 |
| in vitro antiplasmodial, antiamoebic, and cytotoxic activities of some monomeric isoquinoline alkaloids. | twenty-one alkaloids have been assessed for activities against plasmodium falciparum (multidrug- resistant strain k1) in vitro; 18 of these are reported for the first time. two protoberberine alkaloids, dehydrodiscretine and berberine, were found to have antiplasmodial ic(50) values less than 1 m, while seven alkaloids-allocrytopine, columbamine, dehydroocoteine, jatrorrhizine, norcorydine, thalifendine, and ushinsunine-had values between 1 and 10 m. these results are discussed in the context of ... | 2000 | 11141105 |
| inhibition of gene expression in entamoeba by the transcription of antisense rna: effect of 5' and 3' regulatory elements. | down regulation of gene expression by antisense rna is one of the ways to investigate the specific contribution of certain components to the physiology and activities of a cell. a successful inhibition of gene expression in entamoeba trophozoites was achieved in stable transfectants by using hybrid plasmid constructs containing promotors that produce transcripts which do not bind to polysomes. different promotors were found to be required for entamoeba histolytica or entamoeba dispar. in e. hist ... | 2000 | 10717304 |
| compositional pressure and translational selection determine codon usage in the extremely gc-poor unicellular eukaryote entamoeba histolytica. | it is widely accepted that the compositional pressure is the only factor shaping codon usage in unicellular species displaying extremely biased genomic compositions. this seems to be the case in the prokaryotes mycoplasma capricolum, rickettsia prowasekii and borrelia burgdorferi (gc-poor), and in micrococcus luteus (gc-rich). however, in the gc-poor unicellular eukaryotes dictyostelium discoideum and plasmodium falciparum, there is evidence that selection, acting at the level of translation, in ... | 2000 | 10721724 |
| the endocytic pathway in entamoeba histolytica. | we studied the endocytic pathway of entamoeba histolytica using (a) confocal laser scanning microscopy to observe living cells labeled with fluorescent probes and (b) transmission electron microscopy of cells incubated in the presence of gold-labeled proteins or in a cytochemical medium designed for the localization of acid phosphatase activity. images of acridine-orange-labeled cells showed that most of the intracellular vacuoles were acidic and were also labeled with lucifer yellow, a fluoresc ... | 2000 | 11097295 |
| the application of the atomic force microscope to studies of medically important protozoan parasites. | both living and fixed specimens of the medically-important parasitic protozoa, trypanosoma cruzi, toxoplasma gondii, giardia lamblia, entamoeba histolytica, and acanthamoeba spp. were studied by atomic force microscopy (afm). the preparation of fixed specimens was similar to methods used for either scanning or transmission electron microscopy. afm scanning was performed using both contact and tapping modes. a classical fixation procedure utilizing glutaraldehyde followed by ethanol dehydration w ... | 2000 | 11108031 |
| two novel calcium-binding proteins from cytoplasmic granules of the protozoan parasite entamoeba histolytica. | we report on the molecular characterisation of two novel granule proteins of the protozoon and human pathogen entamoeba histolytica. the proteins, which were named grainin 1 and 2, show a considerable structural similarity to calcium-binding proteins, particularly within ef-hand motifs. each grainin possesses three of these putative calcium-binding sites. based on careful inspection of known structures of protein families containing ef-hands, a domain of grainin 1 covering two ef-hand motifs was ... | 2000 | 11113449 |
| [bacteriological quality of human drinking water in high-margination zones in chiapas]. | to analyze the bacteriological quality of drinking water (bqdw) and its association with diarrhoea and intestinal parasites in children 1 to 14 years old, in areas of high levels of poverty in chiapas, mexico. | 2000 | 11125624 |
| socioeconomic status, clinical features, laboratory and parasitological findings of hepatic amebiasis patients--a hospital based prospective study in bangladesh. | socioeconomic status, clinical, laboratory and parasitological features of 31 hospitalized amebic liver abscess (ala) and 8 amebic hepatitis (ah) patients were studied. thirty-seven (94.9%) of the total 39 cases were from low socioeconomic class and 2 (5.1%) were from middle class (p<0.001). sixteen (51.6%) ala and 5 (62.5%) ah patients were admitted with duration of disease for 3 weeks or more. twenty-one (67.7%) ala and 3 (37.5%) ah cases gave no previous history of diarrhea or dysentery. epig ... | 2000 | 11127347 |
| auxotrophy to lipoproteins of entamoeba histolytica cultivated under axenic conditions. | entamoeba histolytica grows in media without serum but with a mixture of aminoacids, vitamins, lipoproteins, free cholesterol, phospholipids and fatty acids called pacsr. the ability of lipoproteins and free lipids to support growth of three e. histolytica strains (hk9, hmi:imss and hm3:imss) was analysed. tubes containing 5 ml culture medium, amino acids, vitamins and either 120-1,200 microg lipoproteins/ml or 0.017-0.10 mg free lipids/ml (predissolved in absolute ethanol) were inoculated with ... | 2000 | 11133106 |
| [amebiasis]. | 2000 | 11043054 | |
| outbreak of amebiasis in a family in the netherlands. | human-to-human transmission of entamoeba histolytica is rare in industrialized countries. we describe an outbreak of amebiasis in a family in the netherlands, demonstrating that even with western standards of hygiene, persistent cyst passage may result in the transmission of e. histolytica to household contacts. if e. histolytica is isolated from a person living in an area of nonendemicity, it may be worthwhile to test all family members for cyst passage. | 2000 | 11049797 |
| entamoeba histolytica: diminution of erythrophagocytosis, phospholipase a(2), and hemolytic activities is related to virulence impairment in long-term axenic cultures. | 2000 | 11052870 | |
| synthesis and antiamoebic activity of new cyclooctadiene ruthenium(ii) complexes with 2-acetylpyridine and benzimidazole derivatives. | reaction of [ru(eta4-c8h12) (ch3cn)2 cl2] with 2-(2-pyridyl) benzimidazole or schiff bases derived from 2-acetylpyridine and s-methyldithiocarbazate, s-benzyldithiocarbazate and thiosemicarbazide leads to form new complexes of the type [ru(eta4-c8h12)(l)cl2] (where l=ligand). in vitro, most of the compounds exhibited potent activity and the ru derivatives 1a [ru(eta4-c8h12)(2-acpy-smdt)cl2], 2a [ru(eta4-c8h12)(2-acpy-sbdt)cl2] and 3a [ru(eta4-csh12)(2-acpy-tsc)cl2] were found more active than me ... | 2000 | 11055329 |
| characteristics of serine acetyltransferase from escherichia coli deleting different lengths of amino acid residues from the c-terminus. | some properties of serine acetyltransferases (sats) from escherichia coli, deleting 10-25 amino acid residues from the c-terminus (satdeltac10-deltac25) were investigated. the specific activity depended only slightly on the length of the c-terminal region deleted. although the sensitivity of satdeltac10 to inhibition by l-cysteine was similar to that for the wild-type sat, it became less with further increases in the length of the amino acid residues deleted. satdeltac10 was inactivated on cooli ... | 2000 | 11055390 |
| intestinal amoebiasis: delayed-type hypersensitivity response in mice. | delayed-type hypersensitive (dth) response was evaluated in c3h/hej mice intestinally infected with entamoeba histolytica. infected and non-infected control mice were challenged with amoebic antigen on day 5, 10, 15, 20, 25, 30, 40, 50, and 60 post-infection. maximum footpad swelling was observed after 24 hours of the challenge. the e. histolytica-infected mice exhibited a dth response on day 5, 15, 20, 25, 40 and 60 post-infection. however, on day 10, 30, and 50, such response was similar to th ... | 2000 | 11057067 |
| arbitrarily primed pcr fingerprinting of rna and dna in entamoeba histolytica. | differences were detected in the gene expression of strains of e. histolytica using rna (rap-pcr) and dna fingerprinting (rapd). analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. the 260 bands generated by using five different primers for rap-pcr, as well as rapd, were employed in the construction of dendograms. the dendogram obtained based on the rapd products permitted the distinction of ... | 2000 | 11058934 |
| early lateral transfer of genes encoding malic enzyme, acetyl-coa synthetase and alcohol dehydrogenases from anaerobic prokaryotes to entamoeba histolytica. | the fermentation enzymes, which enable the microaerophilic protist entamoeba histolytica to parasitize the colonic lumen and tissue abscesses, closely resemble homologues in anaerobic prokaryotes. here, genes encoding malic enzyme and acetyl-coa synthetase (nucleoside diphosphate forming) were cloned from e. histolytica, and their evolutionary origins, as well as those encoding two alcohol dehydrogenases (adhe and adh1), were inferred by means of phylogenetic reconstruction. the e. histolytica m ... | 2000 | 11069669 |
| iron hydrogenases and the evolution of anaerobic eukaryotes. | hydrogenases, oxygen-sensitive enzymes that can make hydrogen gas, are key to the function of hydrogen-producing organelles (hydrogenosomes), which occur in anaerobic protozoa scattered throughout the eukaryotic tree. hydrogenases also play a central role in the hydrogen and syntrophic hypotheses for eukaryogenesis. here, we show that sequences related to iron-only hydrogenases ([fe] hydrogenases) are more widely distributed among eukaryotes than reports of hydrogen production have suggested. ge ... | 2000 | 11070057 |
| reincidence of amebic liver abscess: a case report. | 2000 | 11070200 | |
| a new approach for chemotherapy against entamoeba histolytica. | 2000 | 11070202 | |
| effect of cyclosporine a on entamoeba histolytica. | 2000 | 11070203 | |
| phagocytosis of quinfamide particles by entamoeba histolytica. | 2000 | 11070204 | |
| in vivo inhibition of reduced thiol compounds from entamoeba histolytica hk9 by phenothiazines and related tricyclic drugs. | 2000 | 11070205 | |
| antiamebic activity of aqueous extracts from five medicinal plants from northeastern mexico. | 2000 | 11070206 | |
| antiamebic activity of the metronidazole-zinc association. | 2000 | 11070207 | |
| characterization of plasma membrane-associated antigens of diagnostic and prophylactic importance in entamoeba histolytica. | 2000 | 11070208 | |
| antibodies against entamoeba histolytica hemolysin hly4 in sera from patients with invasive amebiasis. | 2000 | 11070209 | |
| diagnostic tests using idiotype expression in amebiasis. | 2000 | 11070210 | |
| systemic antiamebic antibodies induced orally in mice: detection by an elisa test using preserved entamoeba histolytica-antigens without enzymatic inhibitors. | 2000 | 11070211 | |
| a simple stool concentration method for the detection and preservation of the vegetative forms of entamoeba histolytica/entamoeba dispar. | 2000 | 11070212 | |
| cytological and immunological methods to identify entamoeba histolytica and entamoeba dispar trophozoites in patients from guanajuato, mexico. | 2000 | 11070213 | |
| prevalence of entamoeba histolytica and entamoeba dispar in a highly endemic rural population. | 2000 | 11070214 | |
| differentiation of entamoeba histolytica and entamoeba dispar using pcr-shela and comparison of antibody response. | 2000 | 11070218 | |
| incidence of entamoeba histolytica/entamoeba dispar in international travelers, extracommunitary immigrants, and adopted children. | 2000 | 11070219 | |
| purification and biochemical characterization of three cysteine proteases of entamoeba histolytica with potential application in epidemiologic trials. | 2000 | 11070220 | |
| seroprevalence of anti-entamoeba histolytica antibodies by iha and elisa assays in blood donors from puebla, mexico. | 2000 | 11070221 |