Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the cdna sequence of trichomonas vaginalis virus-t1 double-stranded rna. | trichomonas vaginalis virus (tvv) is a nonsegmented double-stranded (ds) rna virus that infects the pathogenic protozoan t. vaginalis. to study the virus, we cloned the genomic ds rna of a tvv-t1 isolate and obtained a contiguous 4647-bp cdna sequence. two overlapping genes separated by a + 1 reading frame shift were identified on the plus strand but none on the complementary strand rna in this sequence. the upstream gene probably encodes a 75-kda capsid protein, and the downstream gene encodes ... | 1995 | 7831841 |
| maltose utilization by extracellular hydrolysis followed by glucose transport in trichomonas vaginalis. | the amitochondriate parasitic protist trichomonas vaginalis can utilize either glucose or maltose as carbon and energy source. the mechanisms of maltose utilization were explored with uptake experiments using radio-isotope labelled maltose in combination with the silicone-oil centrifugation technique and enzymatic assays measuring maltose hydrolysis. the uptake of maltose label became saturated after 2-3 h. the uptake of maltose as a function of the external maltose concentration was linear at l ... | 1995 | 7845710 |
| trichomonas vaginalis: amoeboid and flagellated forms synthesize similar proteinases. | 1995 | 7895847 | |
| association of post-cesarean delivery endometritis with colonization of the chorioamnion by ureaplasma urealyticum. | to determine if asymptomatic antenatal colonization of the chorioamnion with ureaplasma urealyticum is a risk factor for the development of post-cesarean delivery endometritis. | 1995 | 7898825 |
| evaluation of the effects of albendazole and metronidazole on the ultrastructure of giardia duodenalis, trichomonas vaginalis and spironucleus muris using transmission electron microscopy. | the three closely related parasitic protozoa, giardia duodenalis, trichomonas vaginalis and spironucleus muris, all have very different sensitivities to albendazole and metronidazole. ultrastructural studies reveal that the cytoskeletal elements of the ventral disk in g. duodenalis are affected by albendazole, whereas the other two parasites, neither of which possess this structure, are not affected by albendazole to the same extent. this suggests that albendazole may be having its primary affec ... | 1994 | 7928072 |
| unique double-stranded rnas associated with the trichomonas vaginalis virus are synthesized by viral rna-dependent rna polymerase. | most trichomonas vaginalis isolates are carriers of the multisegmented double-stranded rna (dsrna) virus. in vitro polymerase assays were performed to demonstrate the rna-dependent rna polymerase (rdrp) activity of purified particles. transcripts which comigrated with the dsrnas of the virus were readily detected as synthesized products, indicating viral rdrp activity. in addition, smaller-sized dsrna species, possibly two of approximately 700 bp (s1) and one of 500 bp (s2), were synthesized by ... | 1994 | 7933092 |
| self sampling as part of screening for sexually transmitted diseases in clinic attenders. a preliminary study. | self sampling of the secretions of the female genital tract for microbiological investigations for neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis was assessed in female attenders of the genitourinary medicine clinic in coventry and warwickshire hospital. the purpose of the study was to evaluate the possibility of offering this method of investigation to workers in the sex industry, who are reluctant to attend gum clinics. this pilot study aimed at assessing the feasibilit ... | 1994 | 7948160 |
| antioxidant defences in the microaerophilic protozoan trichomonas vaginalis: comparison of metronidazole-resistant and sensitive strains. | the sensitivity of the microaerophilic protozoan trichomonas vaginalis to oxygen and products of its reduction, and the antioxidant defences employed by this organism, were investigated. studies revealed that this amitochondrial flagellate is sensitive to oxygen tensions above those experienced in situ in the vagina (i.e. > 60 microm) and that metronidazole-resistant strains (cdc 85 and ir78) were more sensitive to elevated oxygen levels than a metronidazole-sensitive isolate (1910). in the pres ... | 1994 | 7952198 |
| carbohydrate metabolism and physiology of the parasitic protist trichomonas vaginalis studied in chemostats. | the parasitic protist trichomonas vaginalis was cultured in chemostats with glucose or maltose as carbon and energy source. the maximum growth rate was about six divisions per day independent of the substrate, and the apparent km for glucose was 0.375 mm. while growing on maltose, the growth rate depended linearly on the maltose concentration, indicating that in contrast to glucose metabolism a diffusion step is rate-limiting to maltose metabolism. cultures were examined over a wide range of gro ... | 1994 | 7952199 |
| adaptation of the carbon metabolism of trichomonas vaginalis to the nature and availability of the carbon source. | the anaerobic parasitic protist trichomonas vaginalis was adapted in chemostats to eight different conditions defined by different growth rates and carbon regimens. glucose or maltose was used as carbon and energy source. cells cultured under well-defined steady states were tested in short-term experiments. the kinetics of glucose and maltose uptake were determined and their glucokinase and alpha-glucosidase activities were measured. uptake in 20 min was measured with radiolabelled glucose and m ... | 1994 | 7952200 |
| expanded polytetrafluoroethylene patch versus braided polyester tape for the repair of incompetent cervix. | this study was done to evaluate pregnant patients with cervical incompetence using an expanded polytetrafluoroethylene (eptfe) patch versus the commonly used braided polyester tape, comparing the rate of local tissue reaction or onset of contractions, or both. | 1994 | 7952453 |
| preparations for aids vaccine evaluations. rate of new hiv infection in a cohort of women of childbearing age in malawi. | women attending the queen elizabeth central hospital in blantyre, malawi, between november 1989 and october 1993 were studied as part of a longitudinal cohort study of mother-to-infant hiv transmission. 694 hiv-seropositive and 687 hiv-seronegative women were enrolled at delivery. in the follow-up phase, women attended the clinic every 3 months for the first 24 months and every 6 months thereafter, where they were administered a questionnaire and underwent pelvic exam for the diagnosis of sexual ... | 1994 | 7865310 |
| subcellular localization of the enzymes of the arginine dihydrolase pathway in trichomonas vaginalis and tritrichomonas foetus. | the enzymes of the arginine dihydrolase pathway were demonstrated in tritrichomonas foetus and their subcellular localization determined for both t. foetus and trichomonas vaginalis. ornithine carbamyltransferase (anabolic and catabolic activities), ornithine decarboxylase and carbamate kinase activity were localized predominately (56-80%) in the non sedimentable fraction of both species. a large proportion (35-40%) of the arginine deiminase was, however, recovered in the large granular fraction ... | 1994 | 7866382 |
| sensitivity of trichomonas vaginalis, tritrichomonas foetus and giardia intestinalis to bacitracin and its zinc salt in vitro. | the activity of bacitracin in vitro against giardia intestinalis, trichomonas vaginalis and tritrichomonas foetus was enhanced 5-10 times by equimolar concentrations of zinc. bacitracin and bacitracin zinc activity was tolerant to either aerobic or anaerobic culture conditions and equally effective against t. vaginalis isolates sensitive or insensitive to metronidazole. this enhancement was not due to zinc toxicity and was zinc dose dependent. | 1994 | 7886780 |
| identification of surface antigen of trichomonas vaginalis. | plasma membrane proteins of a korean isolate of trichomonas vaginalis hy-1 were fractionated for antigen analysis. homogenates of t. vaginalis were fractionated by the differential centrifugation using sucrose step-gradient method. the interface layer from the 25%/45% sucrose was collected as a plasma membrane fraction and its purity was examined by transmission electron microscopy. the antigenicity of plasma membrane fraction was analysed by enzyme-linked immunoelectrotransfer blot technique wi ... | 1994 | 7834241 |
| prevalence of vaginitis, syphilis and hiv infection in women in the orange free state. | to determine the prevalence of vaginitis, syphilis and hiv infection in women in the orange free state. | 1994 | 7839281 |
| condom use relative to knowledge of sexually transmitted disease prevention, method of birth control, and past or present infection. | the purpose of this study was to assess knowledge regarding std spread and prevention, and to assess motivational and behavioral factors that influence the use of condoms to prevent std acquisition among inner-city women at high risk for std infection. in addition, the effect of past and/or current std infection on a woman's knowledge of the mechanism of std acquisition and subsequent use of a condom to prevent std infection was explored. we utilized three inner-city clinics offering family plan ... | 1994 | 7844245 |
| evaluation of two cultural media (cplm & tym) for isolation and maintenance of trichomonas vaginalis stocks in the laboratory. | five hundreds vaginal discharge specimens were inoculated simultaneously in 2 axenic culture media (cplm & tym), in order to compare their ability to isolate and to maintain the growth of t. vaginalis in the laboratory. while both media were found to be equally good in detecting the organisms in vaginal discharges, yet, t. vaginalis stocks were maintained for a longer time in tym medium (one year), than in the cplm medium (2 weeks). the yields of the parasites with different inocula subcultured ... | 1994 | 7844426 |
| trichomoniasis in non-gonococcic urethritis among male patients. | urethral discharge of 415 male patients, age 18-50, were examined for trichomonas vaginalis by direct examination of fresh and giemsa-stained slides and cultivation in cplm media. in this study, 85 (20.7%) specimens out of 415, were found to be positive for trichomoniasis. three groups consisting of 30, 29 and 26 patients each was treated with secnidazole, metronidazole and ornidazole respectively. in the follow up controls, all patients were found to be negative for trichomoniasis and all compl ... | 1994 | 7844427 |
| sexually transmitted diseases, sexual behavior, and cocaine use in inner-city women. | the prevalence of untreated sexually transmitted diseases (stds) was assessed in a cohort of 372 sexually active inner-city women (92% black, 49% us-born) with no history of injection drug use who were recruited in brooklyn, new york, in 1990 and 1991. the presence of stds was assessed via culture, serologic analyses, and medical history. sexual and drug-use histories were obtained, as was a urine sample for toxicologic analysis. thirty-five percent of the women had at least one std (27% trichom ... | 1994 | 7998594 |
| identification and molecular cloning of four cysteine proteinase genes from the pathogenic protozoon trichomonas vaginalis. | the parasitic protozoon trichomonas vaginalis produces multiple forms of cysteine proteinase (cp). the molecular basis for this has now been examined by cloning dna fragments encoding cps. using generic degenerate oligonucleotide primers based on two well-conserved regions within the central region of all eukaryotic cps, several polymerase chain reaction fragments were isolated from t. vaginalis genomic dna and shown to encode different cps. one fragment with a well-represented sequence was used ... | 1994 | 8000542 |
| is trichomonas vaginalis a cause of cervical neoplasia? results from a combined analysis of 24 studies. | we conducted this combined analysis of available data from studies with information on this issue to clarify the association between trichomonas vaginalis infection and cervical neoplasia. | 1994 | 8002180 |
| [trichomonacidal activity of newly synthesized derivatives of benzoizothiazolinon (bit) in vitro]. | trichomonacidal activity in vitro of 2 newly synthesized derivatives of benzoizothiazolinon (bit) in comparison with metronidazole was determined by calculating lethal concentration for 50% trichomonal population (c50). experiments were carried out on 2 strains of t. vaginalis and 2 strains of t. anseris. both preparations showed considerably higher trichomonacidal activity than metronidazole. mean values of trichomonacidal activity of n-morpholinomethyl-1,2-benzoizothiazolinon-3 for t. vaginali ... | 1994 | 8023507 |
| determination of the viability of trichomonas vaginalis using flow cytometry. | in clinical laboratories, viability of trichomonas vaginalis is determined by using light microscopy (differential count of motile to nonmotile organisms). alternative methods are proposed that utilise flow cytometry. under an epifluorescence microscope, live organisms fluorescence intensely green with fluorescein diacetate (fda), whereas dead cells fluoresce orange with propidium iodide (pi). flow cytometric histograms of green versus red fluorescence reveal distinct populations for live and de ... | 1994 | 8026224 |
| a new method for identification of trichomonas vaginalis by fluorescent dna in situ hybridization. | the protozoan flagellate trichomonas vaginalis is responsible for human trichomoniasis, one of the most widespread sexually transmitted diseases in the world. several methods are currently used for laboratory diagnosis, including direct microscopic observation, cell culture, immunological techniques, and more recently, dna probing and gene amplification. this report describes an in situ hybridization technique with specific dna probes labeled with either biotin, rhodamine, or fluorescein for det ... | 1994 | 8027304 |
| sexually transmitted diseases in children: non viral including bacterial vaginosis, gardnerella vaginalis, mycoplasmas, trichomonas vaginalis, candida albicans, scabies and pubic lice. | 1994 | 8039788 | |
| the influence of nonoxynol-9-containing spermicides on urogenital infection. | the most common spermicidal compound in use in north america is nonoxynol-9. barrier methods of contraception used in combination with a spermicidal product help prevent a variety of sexually transmitted diseases. in 1991 the centers for disease control reported a total of 620,478 cases of gonorrhea, 128,569 of syphilis, and 43,672 of acquired immunodeficiency syndrome (aids). the evidence for antimicrobial activity of spermicides against sexually transmitted disease pathogens has been accumulat ... | 1994 | 8051730 |
| [urethro-adnexitis in the man and acute urethral syndrome in the woman. microbiological and immunologic studies of etiologic classification]. | both common pathogens and unconventional, fastidious bacteria, viruses, parasites, and fungi are causative agents in male urethro-adnexitis and in female acute urethral syndrome. uropathogens, neisseria gonorrhoeae, treponema pallidum, mycobacterium tuberculosis, chlamydia trachomatis, mycoplasma spp., haemophilus ducreyi, calymmatobacterium granulomatis, gardnerella vaginalis, anaerobic bacteria, herpes simplex virus type ii (hsv ii), papillomaviruses (hpv), trichomonas vaginalis and candida sp ... | 1994 | 8053087 |
| the intracellular amino acid pools of giardia intestinalis, trichomonas vaginalis, and crithidia luciliae. | the total intracellular amino acid profiles of giardia intestinalis trophozoites, trichomonas vaginalis, and crithidia luciliae were determined by sensitive amino acid analysis. the three protozoan parasites exhibited distinctively different amino acid profiles, but all three were dominated by high concentrations of intracellular alanine. this common feature suggests that alanine synthesis is a major aspect of intermediary metabolism in these protozoan parasites. there were also distinctively di ... | 1994 | 8056076 |
| cytopathologic changes associated with intrauterine contraceptive devices. a review of cervico-vaginal smears in 350 women. | cervico-vaginal smears from 350 iucd users were analysed to ascertain the range of abnormalities induced in the genital tract of these women. alteration of the microbial environment, inflammatory, degenerative, reparative and proplastic epithelial changes were the salient cytological findings. the clinical implications of these are briefly discussed. | 1994 | 8057995 |
| beta-tubulin genes of trichomonas vaginalis. | microtubules, formed by polymerization of alpha and beta-tubulins, are major structural components of the mitotic spindle, cytoskeleton, and flagella, and are also an important target for the antiparasitic benzimidazole drugs. trichomonas vaginalis, a flagellated protozoan responsible for urogenital tract infections in humans, is highly sensitive to certain benzimidazoles in vitro. as a first step towards defining the roles of microtubules in this organism, the regulation of their expression, an ... | 1994 | 8078521 |
| [condyloma acuminatum in female urethra: a case report]. | a case of female intraurethral condyloma acuminata is present in this report. a 52-year-old woman visited our hospital with the chief complaint of miction pain and bloody secretions. thirty seven years previously, she had had a pudental tumor resected which was diagnosed as condyloma acuminata. physical examination revealed two child-finger-head sized, polypoid tumors which arose from about 5 mm inside the external urethral orifice. laboratory findings were negative for rapid plasma reagin card ... | 1994 | 8085526 |
| trichomonas vaginalis: dominant g2 period and g2 phase arrest in a representative of an early branching eukaryotic lineage. | eukaryotic mitotic cell cycles have been extensively studied in yeasts and vertebrate cells but little is known about cell cycle mechanisms in early branches of the eukaryotic lineage. trichomonas vaginalis represents one of the earliest branching eukaryotic lineages available for study. in contrast with most yeasts and vertebrate cells, the t. vaginalis g2 period was prolonged, comprising 50 to 58% of the cell population. hydroxyurea, aphidicolin, and excess thymidine, all of which arrest yeast ... | 1994 | 8087109 |
| epidemiologic analysis of trichomonas vaginalis infection in inflammatory smears. | infections of the lower reproductive tract are common in indian women of reproductive age. hospital-based cytologic screening was undertaken on 63,265 women. the smears were examined for the presence of specific infections, such as candida, herpes simplex virus, human papillomavirus, trichomonas vaginalis (tv) and chlamydia. this paper highlights the prevalence of tv infection in inflammatory cervical smears. among the various infections detected, the rate of tv infection was the highest (5.1%). ... | 1994 | 8091899 |
| the interaction of lactobacillus acidophilus and trichomonas vaginalis in vitro. | recent work with a mouse model of trichomonas vaginalis infection indicated that estrogenized balb/c mice that were preinfected with lactobacillus acidophilus showed a greater duration of t. vaginalis infection as compared to a control group of mice that were not treated with l. acidophilus. to examine the interaction between t. vaginalis and l. acidophilus further we performed in vitro competitive growth assays between the 2 species. although the addition of l. acidophilus to the t. vaginalis c ... | 1994 | 8308658 |
| vaginal discharge--comparison of clinical, laboratory and microbiological findings. | of 101 fertile women (15-50 years of age) consulting in general practice due to vaginal discharge and/or genital malodor, bacterial vaginosis was diagnosed in 34% and vaginal candidiasis in 23%. the presence of chlamydia trachomatis, trichomonas vaginalis, genital herpes virus and neisseria gonorrhoeae was demonstrated in 15%, 9%, 7%, and 1% of cases, respectively. no specific genital tract pathogens were found in 25 patients, where the clinical diagnosis was psychological factors (n = 5), intra ... | 1994 | 7817733 |
| striated fibers in trichomonads: costa proteins represent a new class of proteins forming striated roots. | the production of monoclonal antibodies and the use of biochemical techniques revealed that b-type costa proteins in trichomonads are composed of several major polypeptides with molecular weight detected between 100 and 135 kda similar to those found in the a-type costae. although differences were observed between the two types in their fine structure, we tested whether proteins composing the two costa types belong to the same protein family. a polyclonal antibody produced against the 118 kda co ... | 1994 | 7820860 |
| hydrogenosomes in trichomonads are calcium stores and have a transmembrane electrochemical potential. | 1994 | 7821582 | |
| community based survey of std/hiv infection among commercial sex workers in calcutta (india). part-iv: sexually transmitted diseases and related risk factors. | community based survey on a sample of commercial sex workers in one red light area of calcutta, was carried out to determine prevalence of sexually transmitted diseases (std) including hiv infection and related risk factors. an alarmingly high prevalence of stds (80.56 per cent) but low hiv-seropositivity (1.13 per cent) was observed. candida albicans, neisseria gonorrhoeae and trichomonas vaginalis were detected in 23.24, 13.24 and 11.11 per cent of genital specimens respectively. by tpha test ... | 1994 | 7759801 |
| primary structure of the hydrogenosomal adenylate kinase of trichomonas vaginalis and its phylogenetic relationships. | hydrogenosomal adenylate kinase of the amitochondriate protist, trichomonas vaginalis, has been purified and the sequence of its 39 amino-terminal residues established. based on this sequence and a conserved internal region of the enzyme, a probe was obtained by dna polymerase chain reaction and used to isolate a genomic dna clone containing the gene of this enzyme. this gene exists probably as a single copy in t. vaginalis and is not interrupted by introns. the open reading frame obtained codes ... | 1994 | 7808479 |
| molecular characterization of the alpha-subunit of trichomonas vaginalis hydrogenosomal succinyl coa synthetase. | the anaerobic, parasitic protist, trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. succinyl-coenzyme a synthetase is a hydrogenosomal enzyme which catalyzes the formation of atp via substrate-level phosphorylation. we have characterized genes encoding the alpha subunit of the hydrogenosomal protein succinyl-coenzyme a synthetase (scs). the alpha-scs of t. vaginalis is encoded by a multigene family c ... | 1994 | 7808480 |
| antiprotozoal activities of benzimidazoles and correlations with beta-tubulin sequence. | benzimidazoles have been widely used since the 1960s as anthelmintic agents in veterinary and human medicine and as antifungal agents in agriculture. more recently, selected benzimidazole derivatives were shown to be active in vitro against two protozoan parasites, trichomonas vaginalis and giardia lamblia, and clinical studies with aids patients have suggested that microsporidia are susceptible as well. here, we first present in vitro susceptibility data for t. vaginalis and g. lamblia using an ... | 1994 | 7811023 |
| trichomonas vaginalis: repeated dna target for highly sensitive and specific polymerase chain reaction diagnosis. | trichomoniasis is recognised as a major sexually transmitted disease (std) in the world and may act as an acquired immunodeficiency syndromes (aids) co-factor by enhancing the transmission of human immunodeficiency virus (hiv). diagnosis of trichomonas vaginalis can be achieved by several methods, but sensitive detection means are still lacking. in this study a 2000-bp repeated dna fragment of t. vaginalis was cloned. part of a conserved region of this insert was sequenced, two primers (tvk3 and ... | 1994 | 7812190 |
| genital flora in pregnancy and its association with intrauterine growth retardation. | a study of risk factors for intrauterine growth retardation (iugr) was conducted among a cohort of 13,914 pregnant women enrolled in the multicenter vaginal infections and prematurity study. from 23 through 26 weeks of gestational age, cultures of specimens from the vagina and cervix were done for group b streptococci, neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, candida albicans, gardnerella vaginalis, mycoplasma hominis, ureaplasma urealyticum, and anaerobic gram-negati ... | 1994 | 7814541 |
| giardiavirus-resistant giardia lamblia lacks a virus receptor on the cell membrane surface. | giardia lamblia virus (glv) is a small nonenveloped double-stranded rna virus that infects specifically the parasitic protozoan g. lamblia. among the many collected strains of g. lamblia, a few turn out to be highly resistant to the virus infection. two of these strains, ac and jh, were subjected to electroporation with the rna from glv-infected g. lamblia wb strain. subsequent studies indicated the presence of glv double-stranded rna and glv protein in the electroporated and propagated cells. v ... | 1994 | 7508994 |
| diagnosis of trichomonas vaginalis in male urethritis. | trichomonas vaginalis was diagnosed in 42 (19%) of 227 adult males with urethral discharge. in 27 men (15%) t. vaginalis was isolated together with neisseria gonorrhoeae. non-gonococcal urethritis was diagnosed in 15 patients and t. vaginalis was isolated from 47% of such patients. stained smear preparations, i.e. rapidiff and acridine orange of modified diamond's media, were superior to wet smear microscopy for the identification of t. vaginalis. rapidiff stain was the most sensitive and identi ... | 1994 | 7513101 |
| [infections of the ejaculate by sexually transmissible pathogens]. | certain ejaculate infections can be traced back to sexually transmitted microorganisms, such as neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum and trichomonas vaginalis. to varying extents, these microorganisms cause such classical genital infections as urethritis, epididymitis and prostatitis as well as subclinical genital tract infections. several different pathomechanisms are under discussion for infection of the ejaculate: reduction of spermatogenesis resulting from tes ... | 1994 | 7519803 |
| the mucolytic activity of polyamines and mucosal invasion. | 1994 | 7698417 | |
| parasite polyamine metabolism: targets for chemotherapy. | 1994 | 7698477 | |
| altered purine nucleoside transport as a target for malaria chemotherapy. | 1994 | 7660955 | |
| polypeptides of hydrogenosome-enriched fractions from rumen ciliate protozoa and trichomonads: immunological studies. | the evolution of hydrogenosomes, energy-generating organelles of rumen ciliate protozoa and the flagellate trichomonads has been the subject of much speculation. polypeptides of the hydrogenosome-enriched fractions from the rumen ciliates, dasytricha ruminantium, isostricha spp., polyplastron multivesiculatum and eudiplodinium maggii were separated by sds-page and compared to analogous polypeptide preparations from tritrichomonas foetus. immunoblotting with antisera specific to the hydrogenosome ... | 1994 | 8181726 |
| similarity between a ubiquitous promoter element in an ancient eukaryote and mammalian initiator elements. | to identify regulatory elements that play a role in transcription initiation in ancient eukaryotes, we have analyzed the upstream regions of protein-coding genes from trichomonas vaginalis, one of the most ancient eukaryotes studied to date. characterization of seven protein-coding genes from this protist invariably revealed the presence of a highly conserved dna sequence motif immediately upstream of the coding region. this 13-nt motif was shown to surround and contain precise sites for transcr ... | 1994 | 8183951 |
| trichomonas vaginalis with a double-stranded rna virus has upregulated levels of phenotypically variable immunogen mrna. | some isolates of trichomonas vaginalis carry a double-stranded rna virus (tvv) and undergo phenotypic variation between surface expression and cytoplasmic expression of a prominent immunogen (p270). only trichomonads with tvv express p270 on the surface. northern (rna) blots using a specific cdna encoding the repeat element of the phenotypically varying p270 immunogen as a probe showed accumulation of p270 transcript only in isolates with tvv (v+) in contrast to isolates without the virus (v-). ... | 1994 | 8189538 |
| identification of the lipid moiety and further characterization of the novel lipophosphoglycan-like glycoconjugates of trichomonas vaginalis and trichomonas foetus. | the lipid moiety of the lipophosphoglycan (lpg)-like glycoconjugates of trichomonas vaginalis and trichomonas foetus, parasites of the urogenital tract of human and cattle, respectively, has been isolated and characterized by a combination of enzymatic and chemical degradation, chromatography, and mass spectrometry. the carbohydrate composition of the glycan inositol lipid core is also reported. the glycan inositol core of trichomonad glycoconjugates is unique in having more than one glcn and is ... | 1994 | 8135538 |
| molecular cloning of hydrogenosomal ferredoxin cdna from the anaerobic amoeboflagellate psalteriomonas lanterna. | anaerobic parasitic and free living protozoa and anaerobic rumen fungi often contain a characteristic organelle, the hydrogenosome. recently obtained molecular data show that hydrogenosomes in parasitic protozoa probably use a mitochondria-like protein targeting mechanism, whereas for hydrogenosomes in fungi a microbody-like mechanism is inferred. here we present, to our knowledge, the first sequence data of a hydrogenosomal protein in a free-living anaerobic protozoan. it is shown that ferredox ... | 1994 | 8286402 |
| [hemolytic activity of various strains and clones of trichomonas vaginalis and tritrichomonas suis]. | hemolytic activity of 7 isolates and 2 clones of trichomonas vaginalis and 1 isolate and 2 clones of tritrichomonas suis was determined using incubation with erythrocytes. t. vaginalis hemolyzed all human blood groups, and no correlation between pathogenicity and hemolytic activity was observed and no hemolysin released by the parasite could be detected. no hemolytic activity was observed with strains and clones of t. suis against erythrocytes from all blood groups and with swine erythrocytes. | 1994 | 7709097 |
| evaluation of affirm vp microbial identification test for gardnerella vaginalis and trichomonas vaginalis. | a commercial system (affirm vp microbial identification test; microprobe corp.) for detection of vaginal pathogens was evaluated with 176 consecutive women attending a sexually transmitted disease clinic for genital complaints. vaginal swab specimens were used for culture of gardnerella vaginalis and trichomonas vaginalis, preparation of a vaginal smear for gram stain interpretation, and wet mount evaluation. an additional swab was used to evaluate the 30-min nonisotopic oligonucleotide probe te ... | 1994 | 8126171 |
| detection of bovine trichomoniasis with a specific dna probe and pcr amplification system. | trichomoniasis is a widespread, economically important venereal disease of cattle which causes infertility and abortion. effective control of trichomoniasis has been impeded by the insensitivity of traditional diagnostic procedures, which require the isolation and cultivation of the parasite, tritrichomonas foetus, from infected cattle. we developed a 0.85-kb t. foetus dna probe by identifying conserved sequences in dnas from t. foetus that were isolated from cattle in california, idaho, nevada, ... | 1994 | 8126211 |
| [biocenosis of the vagina and infection with chlamydia trachomatis]. | the authors have examined 379 women for infection by chlamydia trachomatis, performing vaginal smears and routine bacterial cultures. c. trachomatis was discovered in 33.2% of the women, most frequently accompanied by erosion of the collum, cervicitis and cin-ca. women with c. trachomatis had the following levels of vaginal purity: 1-44.4%, 2-23.0%, 3 and 4-29.4%, 3.2% -leukocytes only. leukocytes were present in significantly more women with level 1 of vaginal purity than in the control group. ... | 1994 | 7967934 |
| analysis of the extracellular proteases of trichomonas vaginalis. | we have previously isolated two extracellular cysteine proteases (60 and 30 kda, respectively) from the cell filtrate of an isolate of trichomonas vaginalis. in this study the clinical presentation of 12 clinical isolates of t. vaginalis was correlated with their protease activity. all 12 isolates produced a 60-kda protease as demonstrated by immunoblotting. however, only 5 of 12 isolates produced a 30-kda protease in the extracellular filtrate. protease activity did not differentiate between is ... | 1994 | 7971921 |
| [comparison of latex agglutination test with conventional methods for diagnosis of trichomoniasis]. | swabs of the genito-urinary tract of 638 women were taken and subsequently examined with the use of the latex agglutination test (lat). the results were compared with wet mounts and cultures. the sensitivity of these methods were: 98, 30.7, and 46.1; the specificity: 99.8, 100, and 100; the usefulness: 99.5, 94.4, and 95.6, respectively. in acute trichomoniasis the results of lat and the conventional methods were almost identical but in the chronic and asymptomatic forms of the disease, the resu ... | 1994 | 7975652 |
| analysis of a single-domain p-glycoprotein-like gene in the early-diverging protist trichomonas vaginalis. | we have characterized a gene encoding an adenosine triphosphate (atp) binding cassette (abc) transmembrane protein from trichomonas vaginalis, an early-diverging protozoan parasite. this gene, tvpgp1, encodes a 589-amino acid protein with an amino-terminal hydrophobic region, 6 potential membrane-spanning segments and a carboxy-terminal atp binding site. tvpgp1 is most similar in sequence to mammalian p-glycoproteins, 170 kda transport proteins which are frequently overexpressed in multiple drug ... | 1994 | 7984175 |
| monocyte-derived interleukin-8 involved in the recruitment of neutrophils induced by trichomonas vaginalis infection. | 1994 | 7996015 | |
| the role of microscopy in the diagnosis of sexually transmitted infections in women. | microscopy of genital secretions is routinely performed in female patients attending genitourinary medicine clinics. it diagnoses only 50-70% of gonorrhoea, 40-80% of trichomoniasis and has no value in the diagnosis of chlamydial infection. this study was therefore conducted to reassess the role of routine microscopy in female patients. | 1994 | 8206469 |
| trichomoniasis: its clinical significance and diagnostic challenges. | trichomoniasis is a significant std worldwide. clinical studies have demonstrated that trichomoniasis may have serious side effects both in pregnancy and in a predisposition to retrovirus infection. therefore it is important that clinicians request the most accurate diagnostic test available. this is mandated by the significant percentage of both male and female patients that may be asymptomatic. numerous clinical studies have indicated that the most sensitive test for t. vaginalis is with cultu ... | 1994 | 10163483 |
| a study on the in vitro susceptibility of trichomonas vaginalis to metronidazole. | the susceptibility of 32 clinical isolates of trichomonas vaginalis to metronidazole has been studied under aerobic and anaerobic conditions. of 32 patients with vaginal trichomoniasis, 27 (84%) were cured by a standard metronidazole treatment regimen (200 mg thrice daily for seven days). the geometric means of minimum inhibitory concentration (mic) under aerobic and anaerobic conditions for these isolates were 2.0 and 0.4 micrograms/ml, respectively; the geometric means of minimum lethal concen ... | 1994 | 9747331 |
| intestinal parasitic infections and swamp development in sierra leone. | the prevalence of entamoeba histolytica, giardia lamblia and other intestinal and urogenital parasites were assessed in five inland valley swamp (ivs) development faming communities in the moyamba district, south-central sierra leone. stool and urine samples were submitted by 1106 individuals and examined by the iron-haematoxylin staining and the formalin-ether concentration techniques for faecal sample and centrifugation method for the urine samples. the overall parasitic infection rate was 61. ... | 1994 | 12153344 |
| trichomonas vaginalis: diagnosis and clinical characteristics in pregnancy. | the objectives of this study were to 1) determine the prevalance and characterize the symptomatology of trichomonas vaginalis (tv) infection in pregnant women on entry into prenatal care in an inner-city population; 2) compare conventional microscopic methods vs. culture techniques in diagnosing tv in both symptomatic and asymptomatic pregnant patients; and 3) correlate wet mount microscopic and microbiologic characteristics of varying manifestations of trichomoniasis. | 1994 | 18472879 |
| trichomonas vaginalis in pregnancy. | 1994 | 18475347 | |
| detection of trichomonas vaginalis using the polymerase chain reaction in pregnant and non-pregnant women. | trichomonas vaginalis vaginal infections are often both asymptomatic and difficult to detect by current methods. we evaluated the ability of a newly developed polymerase chain reaction (pcr) assay to identify t. vaginalis in vaginal samples from pregnant and non-pregnant women. | 1994 | 18475360 |
| isolation, identification and sensitivity pattern of microorganisms isolated from the urine of pregnant women. | the present studies were conducted to detect and identify the microorganism from the urine of pregnant women having urinary tract infection. the antibiotic susceptibility of these isolated microorganisms was also determined. the microorganisms found responsible for the infection were bacteria, fungi, yeast and protozoa. among the bacteria two were identified as gram-positive cocci i.e. staphylococcus aureus and s. epidermidis, the remaining two were gram-negative bacilli which were escherichia c ... | 1994 | 16414749 |
| the existence of lipophosphoglycanlike molecules in trichomonads. | the trichomonods, trichomonas vaginalis and tritrichomonas foetus, appear to express novel lipophosphoglycon (lpg) like glycoconjugates on their cell surface, which are structurally distinct from leishmania lpgs'. in this article, bibhuti singh discusses the structural and cellular aspects o f these molecules, and speculates on their biological significance. | 1994 | 15275484 |
| the female condom: std protection in the hands of women. | in 1993, the us food and drug administration (fda) approved the female condom reality. it provides better protection against sexually transmitted diseases (stds) than does the male condom. the polyurethane sheath is more resistant to tears and covers the vagina, cervix, and vulva. since it does not depend on erection of the penis, the female condom protects women from stds/hiv that can be transmitted through the pre-ejaculate. in light of the aids epidemic, fda approved the female condom based o ... | 1993 | 12288250 |
| std and contraception. | pelvic inflammatory disease (pid) is caused by several pathogens. it is difficult to diagnose on clinical grounds alone. the best diagnostic tool for pid is laparoscopy. women using contraceptives tend to be more sexually active and to have multiple sex partners than their counterparts and hence are at a greater risk of sexually transmitted diseases (stds). oral contraceptives (ocs) appear to protect against pid but may increase the risk of lower genital tract chlamydial infection. among oc ... | 1993 | 12318983 |
| [frequency of vaginal trichomoniasis and candidiasis and its relation to the clinical profile]. | eight hundred samples of vaginal swabs were taken from women with active sexual life attending the cervicouterine cancer opportune detection service at the familial medicine unit no. 18 of the national institute of social security, to study the incidence and prevalence of vaginal trichomoniasis, the presence of yeasts of the genus candida and their relationships with clinical features of vulvovaginitis. identification of parasites was made by means of direct examination with light microscope; 14 ... | 1993 | 8209117 |
| synthesis of some 4-oxo-delta 2-thiazolin-2-ylhydrazones as potential antiprotozoal agents. | several 4-oxo-delta 2-thiazolin-2-ylhydrazone derivatives 6 (6-1 - 6-19) derived from the cyclization reaction between the thiosemicarbazones 5 and homologous alpha-chloroacids were prepared. only compound 6-2 was found to be active against entamoeba histolytica and trichomonas vaginalis. | 1993 | 8216678 |
| killing of trichomonas vaginalis by complement-mediated lysis is not associated with the presence of trichomonas vaginalis virus. | we have examined the possible relationship between trichomonad killing by human serum and the presence of virus-encoded double-stranded ribonucleic acid in t. vaginalis (tvv). indirect immunofluorescence assay revealed that non-immune serum (nis) and t. vaginalis-immune serum (tvis) had immunoglobulin g (igg) antibody titres of 1:8 and 1:256, respectively, against t. vaginalis. among the 12 isolates of t. vaginalis examined, 9 were infected with tvv. upon long-term (> 9 months) culture, of the 9 ... | 1993 | 8225773 |
| multiple double-stranded rna segments are associated with virus particles infecting trichomonas vaginalis. | previous studies demonstrated that some isolates of the sexually transmitted protozoan trichomonas vaginalis are infected with a nonsegmented, double-stranded rna (dsrna) virus. a reexamination of the total dsrna extracted from several virus-harboring isolates indicated the presence of at least three dsrnas with sizes ranging from 4.8 to 4.3 kbp. the double-stranded nature of each of the three segments was determined by hybridization experiments using riboprobes of opposite polarities obtained f ... | 1993 | 8230417 |
| design, synthesis and biological study of new antiparasitic spiroarsoranes. | thirty-eight new spiroarsoranes were synthesized after structure-activity relationship studies from the first series. these compounds were predicted to cross more easily the membrane of protozoae or the cuticle of nematodes and to reach their biological target with efficiency. the spiroarsoranes were evaluated for their antiparasitic properties, on helminths and protozoa models in regard of their parent arsonic acids. the following parasite models were used: entamoeba histolytica and trichomonas ... | 1993 | 8240448 |
| hiv and other sexually transmitted diseases at a rural hospital in zimbabwe. | to define the epidemiological characteristics of std patients attending an outpatient clinic in rural zimbabwe, to examine the aetiologic agents causing infection and to determine their relationship with hiv infection. | 1993 | 8244351 |
| the association between sexually transmitted pathogens and cervical intra-epithelial neoplasia in a developing community. | objective--to determine the association of sexually transmitted pathogens in women with cervical intra-epithelial neoplasia (cin). setting--an urban tertiary referral hospital serving a large indigent developing community. participants--48 women attending a colposcopy clinic and 49 women attending a family planning clinic. methods--vaginal, endocervical, rectal swab specimens and sera were collected for the detection of sexually transmitted pathogens. cervical cytology was performed on all patie ... | 1993 | 8244352 |
| enhancement of the antitrichomonal activity of 5-nitroimidazole derivatives by hydrogen peroxide. | the in vitro sensitivity of nine trichomonas vaginalis isolates to commonly employed 5-nitroimidazoles (metronidazole, nimorazole, ornidazole and tinidazole) was evaluated in absence and in presence of sub-inhibitory concentrations of hydrogen peroxide (h2o2). co-incubation with h2o2 and 5-nitroimidazole compounds decreased the mic values of the strains exhibiting cross-resistance to these drugs. it was suggested that h2o2 produced in the inflammatory process during trichomonal infection could e ... | 1993 | 8264426 |
| [trichomonas vaginalis in women attending gynecology clinics]. | in this study trichomonas vaginalis was investigated in vaginal swab specimens from 234 women attending to the gynecology outpatient clinics with various reasons. trichomonas vaginalis was detected by wet mount in 8 women (3.4%) and, by culture using modified diamond's medium in 9 women (3.8%). of 9 cases only 2 were suspected for trichomoniasis by gynecological examination. | 1993 | 8264450 |
| trichomonal abscess of the median raphe of the penis. | 1993 | 8270343 | |
| chronic perianal ulcerations: role of trichomonas vaginalis? | 1993 | 8282308 | |
| comparative pathogenicity of trichomonas vaginalis isolated from symptomatic and asymptomatic cases. | pathogenicity of 19 isolates of trichomonas vaginalis obtained from vaginal specimens were studied in the murine model by intraperitoneal inoculation. sixteen isolates were recovered from the females with various clinical conditions and 3 isolates were from normal healthy females. pathogenicity level of these isolates were studied by inoculating 5 mice per isolate through intraperitoneal route and the animals were sacrificed on the 10th day post-inoculation. in general, all the isolates recovere ... | 1993 | 7996493 |
| antitrichomonal action of emodin in mice. | emodin, an active component contained in the root and rhizome of rheum palmatum l. (polygonaceae), was found to have an inhibitory effect on the pathogenicity of trichomonas vaginalis in mice. emodin delayed the development of subcutaneous abscesses due to infection of this parasite. also, it cures the intravaginal infection of trichomonads through oral administration. in cell cultures, it reduced the cytotoxic effect of this parasite towards mammalian cells. this inhibition was markedly reverse ... | 1993 | 8133650 |
| a trichomonas vaginalis cdna with partial protein sequence homology with a plasmodium falciparum excreted protein abra. | we have previously isolated two extracellular cysteine proteases (60 kda, 30 kda) from the cell filtrate of an isolate of trichomonas vaginalis. all isolates tested produced a 60 kda protease as demonstrated by immunoblot with cross-reacting rabbit serum. a t. vaginalis cdna library was constructed using lambda gt11. a 760 bp t. vaginalis specific cdna was sequenced and demonstrates partial protein sequence homology with an extracellular cysteine protease of plasmodium falciparum. these results ... | 1993 | 8298655 |
| [research of antiparasitic agents: 1(nitrophenyl)-3-(methyl-3-indolyl)-prop-2-ene-1-ones (nitroindolylchalcones)]. | the synthesis and the study of the antiparasitic properties of various 1-(nitro-phényl)-3-(méthyl-3-indolyl)-prop-2-ene-1-ones (nitroindolylchalcones) show interesting antiprotozoal activities on e. histolytica and t. vaginalis. no significative activity appears in vivo with animals on helminthes. | 1993 | 8154794 |
| vaginal yeast infections in diabetic women. | two hundred and three diabetic women (89 with and 114 without genital symptoms) were examined for the presence of yeasts and trichomonas vaginalis. yeasts were isolated from the vaginas of 35.5% of patients and were more common in the symptomatic group (48.0%) than the asymptomatic group (25.4%; p < 0.05). candida albicans was isolated from 12.8% of all patients and showed a significant association with pruritus vulvae (p < 0.05). a significant association was also shown between the presence of ... | 1993 | 8191325 |
| protein kinases in divergent eukaryotes: identification of protein kinase activities regulated during trypanosome development. | the role of protein kinases in organisms that diverged early in the eukaryotic lineage is relatively unexplored. in this study, we determined that primitive parasitic protozoa possess multiple protein-serine kinases and inferred the presence of protein-tyrosine kinases through sensitive immunoblotting techniques. to further explore the role of protein kinases in parasite development, we examined the activity of eight renaturable protein kinases during the life cycle of the protozoan parasite try ... | 1993 | 7681984 |
| [a rapid staining method for the study of the flagellar and round forms of trichomonas vaginalis from culture]. | this method has been developed for the routine observation of the different forms of trichomonas vaginalis in axenic culture with a low rate of cell loss. this rapid dyeing process is especially suitable for the examination of the ratio between the flagellated and round forms of the parasite. as a result of this staining one can not see fine cell details but can firmly distinguish between flagellated forms and multi-nucleated, multi-flagellated forms (i.e. dividing cells forms). | 1993 | 7685223 |
| drug-resistant trichomonas vaginalis. | 1993 | 7689025 | |
| sexual transmission of hepatitis c virus and hiv-1 infection in female intravenous drug users. | we examined the relationship between the presence of antibody to hcv and sexually transmitted diseases in 151 female, intravenous drug users aged 17-43 years. anti-hcv was present in 49 of 66 hiv-1-negative women (74.2%), and 63 of 85 hiv-1-positive women (74.1%). anti-hcv seropositivity was significantly related to sexually transmitted infections (ureaplasma urealyticum infections excluded) in hiv-1-seronegative women, but not in hiv-1-positive women. also, in hiv-1-negative subjects. trichomon ... | 1993 | 7691651 |
| neotran--a new double-active pessary for the treatment of vaginitis. | the common causes of vaginitis have changed in recent years. infection with trichomonas vaginalis is much less frequent than it used to be. the most common causes of vaginitis are candidiasis and bacterial vaginosis due to infection with mixed bacterial flora. in general practice, treatment of vaginitis is often begun before microbiological confirmation of the diagnosis is available. a vaginal pessary has been formulated to provide an effective therapy in all three forms of vaginitis mentioned a ... | 1993 | 8319819 |
| trichomonas vaginalis haemolysis: evidence of functional pores formation on red cell membranes. | we have investigated the mechanisms used by trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mm egta. osmotic protection experiments using carbohydrates with different molecular diameters as protectants demonstrated that the cytolytic activity of t. vaginalis is inhibited in 75 mm stachyose. on the basis of our data, we hypothesize a cytopathic mechanism mediated by t ... | 1993 | 8319880 |
| purification of cysteine proteinases from trichomonads using bacitracin-sepharose. | bacitracin affinity chromatography has been used to purify proteinases of the parasitic protozoon tritrichomonas foetus. it proved superior to other affinity chromatography methods we have tested for the purification of trichomonad proteinases and should prove a useful procedure for purifying cysteine proteinases from these parasites and other parasitic protozoa. the main cysteine proteinases of t. foetus were purified over 100-fold to be free from the majority of other cell proteins. about 90 m ... | 1993 | 8319888 |
| interaction between facilitated diffusion of glucose across the plasma membrane and its metabolism in trichomonas vaginalis. | the parasitic protist trichomonas vaginalis transports glucose across the plasma membrane by facilitated diffusion. the km of the transporter for glucose was 1.6 mm. the uptake of labelled glucose in a minimal medium not allowing growth reached saturation only after 2.5 h, indicating the turnover of storage carbohydrate. organisms grown on glucose showed higher activities both of the transporter and of the subsequent metabolic pathway than organisms grown on maltose. at low external glucose conc ... | 1993 | 8319891 |
| trichomonas vaginalis and early evolving dna and protein sequences of the cdc2/28 protein kinase family. | the human sexually transmitted parasite trichomonas vaginalis is a representative of one of the three earliest evolving eukaryotic lineages. we investigated whether t. vaginalis has dna sequences and peptides related to cell division control molecules universal among yeasts and higher eukaryotes. a t. vaginalis cell division control (cdc2/28) homologue was amplified by the polymerase chain reaction and sequenced. the absolute similarity with other cdc2/28 genes was 47%, with conservative replace ... | 1993 | 8326863 |
| trichomonas vaginalis: strain differences in adhesion to plastic and virulence in vitro and in vivo. | trichomonas vaginalis isolated from clinical cases were grown axenically in tyi-s-33 medium. various strains of this flagellate showed different adhesion characteristics in medium containing bovine serum or cohn fractions thereof. these were not inversely related to the time in axenic culture. the adsorption of serum components to the parasites was assessed in relationship to adhesion but in many cases was probably not adhesion-related. all strains tested for virulence in vitro were almost equal ... | 1993 | 8327454 |