Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| [antigen retrieval immunohistochemistry under the influence of ph value and time]. | to find the best antigen retrieval buffer and retrieval time for the immunohistochemical staining of p504s, p63, cd10, and ki-67. | 2005 | 15841154 |
| a novel filtration-based processing method of liquid cytology specimens for human papillomavirus dna testing by hybrid capture ii. | we evaluated a more efficient method of processing liquid-based cervical cytology specimens for human papillomavirus (hpv) dna testing by hybrid capture ii (hcii). aliquots were made from 701 specimens in the following sequence: 4.0, 2.0, 1.0, 0.5, and 1.5 ml. the 4.0-ml aliquot was processed by the standard method (stp), and half of the processed material was tested by hcii. other aliquots were processed with a new, filtration-based processing method (npm). the 2.0-ml npm aliquot had hcii test ... | 2005 | 15842050 |
| human papillomavirus anogenital disease in hiv-infected individuals. | human papillomavirus (hpv) is one of the most common sexually transmitted infections and a significant cause of anogenital malignancies, precancer lesions, and cutaneous disease. human immunodeficiency virus (hiv)-positive individuals have a higher prevalence of hpv infection and hpv-associated anogenital disease compared to age-matched hiv-negative controls. data suggest that there has been little reduction in hpv-associated disease since the introduction of highly active antiretroviral therapy ... | 2005 | 15842614 |
| e7-expressing hacat keratinocyte cells are resistant to oxidative stress-induced cell death via the induction of catalase. | cervical carcinoma is one of the most prevalent cancers in women worldwide, and human papillomavirus (hpv) type 16 is the most common agent linked to human cervical carcinoma. in order to identify various relevant factors affected by the e7 oncogene, we established a stable cell line, which constitutively expressed e7 using the hacat human keratinocyte cell line. the increased expression and activity of catalase in the e7-expressing hacat cells (hacat/e7) were verified via matrix-assisted laser ... | 2005 | 15852342 |
| hpv-16 l1 vlp vaccine elicits a broad-spectrum of cytokine responses in whole blood. | here, we evaluated innate and adaptive immune system cytokine responses induced by hpv-16 l1 vlp in whole blood (wb) cultures from individuals receiving the vaccine (n=20) or placebo (n=4) before and after vaccination. 11 cytokines were measured: il-1beta, il-2, il-4, il-5, il-6, il-8, il-10, il-12, ifn-gamma, tnf-alpha, and gm-csf using multiplex bead arrays. cytokine profiles from wb samples clearly discriminated between vaccine and placebo recipients and between pre and post-vaccination respo ... | 2005 | 15855014 |
| human papillomavirus type 16 e6 and e7 oncoproteins upregulate c-iap2 gene expression and confer resistance to apoptosis. | inhibition of apoptosis plays an important role in the cellular immortalization and transformation induced by e6 and e7 oncoproteins of human papillomavirus (hpv). here, we report that the transcription of the inhibitor of apoptosis gene, cellular inhibitor of apoptosis protein 2, (c-iap2), is significantly upregulated in hpv16 e6/e7-immortalized human oral keratinocytes (hok16e6e7). overexpression of e6/e7 from the high-risk hpv16 or 18, but not from the low-risk hpv6, activated c-iap2 promoter ... | 2005 | 15856013 |
| marginal periodontium as a potential reservoir of human papillomavirus in oral mucosa. | although human papillomaviruses (hpvs) are associated with a number of proliferative epithelial lesions including squamous cell malignancies, they can also be detected in the normal oral mucosa in 10% to 20% of the adult population. however, the point of entry and the site of replication of hpv in the oral cavity are not known. since the gingival pocket is the only site in the oral mucosa where basal cells, known to be targets of hpv at other mucosal sites, are normally exposed to the environmen ... | 2005 | 15857068 |
| variant upstream regulatory region sequences differentially regulate human papillomavirus type 16 dna replication throughout the viral life cycle. | while the central role of the viral upstream regulatory region (urr) in the human papillomavirus (hpv) life cycle has been well established, its effects on viral replication factor expression and plasmid replication of hpv type 16 (hpv16) remain unclear. some nonprototypic variants of hpv16 contain altered urr sequences and are considered to increase the oncogenic risk of infections. to determine the relationship between viral replication and variant urrs, hybrid viral genomes were constructed w ... | 2005 | 15857977 |
| p16ink4a, cdc6, and mcm5: predictive biomarkers in cervical preinvasive neoplasia and cervical cancer. | to analyse and compare expression patterns of three potential biomarkers-p16(ink4a), cdc6, and mcm5-and evaluate their use as predictive biomarkers in squamous and glandular cervical preinvasive neoplasia. | 2005 | 15858126 |
| establishment and characterization of a human first-trimester extravillous trophoblast cell line (tev-1). | research into the biology of human trophoblast invasion has been hampered by a lack of in vitro models. the aim of this study was to establish and characterize a human extravillous trophoblast cell line from the first-trimester placenta. | 2005 | 15866109 |
| detection of human papillomavirus type 16 in bowen's disease of the web-space of the foot. | 2005 | 15868648 | |
| human papillomavirus infection and p16(ink4a) protein expression in vulvar intraepithelial neoplasia and invasive squamous cell carcinoma. | vulvar intraepithelial neoplasia (vin) is defined histopathologically by distinctive abnormalities of cellular maturation and differentiation. the purpose of this study was to investigate the functional properties of vin related to expression of p16(ink4a) protein as well as to detection of human papillomavirus (hpv) type 16 by real-time polymerase chain reaction (rt-pcr) analysis. | 2005 | 15870532 |
| papillomavirus infection in rural women in southern india. | to investigate the prevalence of, and the risk factors for, cervical infection with 44 types of human papillomavirus (hpv) in a rural area in the dindigul district, tamil nadu, india, we interviewed and obtained cervical cell samples from 1891 married women aged 16-59 years. hpv prevalence was 16.9% overall and 14.0% among women without cervical abnormalities, or 17.7 and 15.2%, respectively, age-standardised to the world standard population. in all, 21.9% of infections involved more than one hp ... | 2005 | 15668709 |
| association of cottontail rabbit papillomavirus e6 oncoproteins with the hdlg/sap97 tumor suppressor. | papillomaviruses are small dna viruses that infect epithelial tissues and cause warts. human papillomavirus (hpv) infection is the primary risk factor for the development of cervical cancer. the e6 and e7 oncogenes are the only genes consistently expressed in hpv-positive cervical cancer cells. cottontail rabbit papillomavirus (crpv) induces papillomas and carcinomas on cottontail and domestic rabbits and provides an excellent animal model of hpv infection and vaccine development. crpv encodes t ... | 2005 | 15669058 |
| human papillomavirus (hpv) genotyping by hpv dna chip in cervical cancer and precancerous lesions. | the human papillomavirus (hpv) is a well-known cause of cervical cancer. hpv tests are used as an adjunct test to decrease the false-negative rate of cytological screening. however, attempts are being made to replace the cytological screening with hpv tests. therefore, this study was performed to examine the possibility of using hpv tests as screening test. | 2005 | 15670301 |
| cellular process classification of human papillomavirus-16-positive siha cervical carcinoma cell using gene ontology. | this study utilized mrna differential display and the gene ontology (go) analysis to characterize the multiple interactions of a number of genes involved in human papillomavirus (hpv)-16-induced cervical carcinogenesis. we used hpv-16-positive cervical cancer cell line (siha) and normal human keratinocyte cell line (hacat) as a control. each gene has several biological functions in the go, and hence, we chosen the several functions for each gene. and then, the specific functions were correlated ... | 2005 | 15670303 |
| polymorphism in the e6 gene of human papillomavirus type 16 in the cervical tissues of korean women. | the aim of this study was to identify sequence variants in the hpv 16 e6 gene in korean women and to examine the possible association between these sequence variants and cervical cancer development. we examined the hpv 16 dna of 215 patients with no cervical disease (ncd) (n = 105) or with cervical neoplasia (n = 110) [cervical intraepithelial neoplasia (cin), n = 61; invasive cervical carcinoma (icc), n = 49] using the nested polymerase chain reaction (pcr) and pcr-directed sequencing methods. ... | 2005 | 15670304 |
| results of a phase ii double-blinded randomized clinical trial of difluoromethylornithine for cervical intraepithelial neoplasia grades 2 to 3. | our purpose was to conduct a double-blinded randomized trial of difluoromethylornithine (dfmo) at 0.125, 0.5 gm/m2, versus placebo in the treatment of cervical intraepithelial neoplasia (cin) grades 2 to 3. a promising phase i study has shown histopathologic responses at these dose levels. | 2005 | 15671570 |
| genetic abnormalities and hpv status in cervical and vulvar squamous cell carcinomas. | cervical and vulvar cancers are diseases of the female lower genital tract, and high-risk human papillomavirus (hpv) infection is the most important risk factor for the development of both cancers. however, it is clear that additional genetic events are necessary for tumor progression, particularly in hpv-negative cases. we detected the presence of high-risk hpv16 and hpv18 genomes by gene-specific polymerase chain reaction and searched for common genetic imbalances by comparative genomic hybrid ... | 2005 | 15676146 |
| verucciform xanthoma of the penis not associated with human papillomavirus infection. | verruciform xanthoma (vx) is a rare lesion with a predilection for oral mucosa. only 16 cases of vx of the penis have been reported. histologically, vx lesions in different locations are identical; however, the etiology is controversial. previous studies have reported the presence of human papillomavirus (hpv) in vx of the skin. the purpose of this study was to determine whether hpv is a causative agent in this rare case of vx of the penis. microscopically, the lesion demonstrated prominent verr ... | 2005 | 15737058 |
| regulation of apoptosis by the papillomavirus e6 oncogene. | infection with human papillomaviruses is strongly associated with the development of multiple cancers including esophageal squamous cell carcinoma. the hpv e6 gene is essential for the oncogenic potential of hpv. the regulation of apoptosis by oncogene has been related to carcinogenesis closely; therefore, the modulation of e6 on cellular apoptosis has become a hot research topic recently. inactivation of the pro-apoptotic tumor suppressor p53 by e6 is an important mechanism by which e6 promotes ... | 2005 | 15742391 |
| abstracts of the 24th annual convention of indian association for cancer research and international symposium on human papillomavirus and cervical cancer. february 9-12, 2005, noida, india. | 2005 | 15744933 | |
| human papilloma virus, papanicolaou smears, and the college female. | this article discusses human papilloma virus (hpv), papanicolaou smears and the rational for new screening guidelines. | 2005 | 15748929 |
| genitourinary issues in the male college student: a case-based approach. | five percent of all ambulatory visits by men 18 years of age or older include genitourinary symptoms as a reason for the visit. in this article, using typical, unusual, or otherwise instructive cases, the authors review a select group of genitourinary issues in the college-age male. warts (human papilloma virus), is the most common sexually transmitted infection, and it may mimic other disease. testicular cancer is one of the most serious diseases to confront health care providers. varicoceles a ... | 2005 | 15748931 |
| the epidemiology of human papillomavirus infections. | infection with oncogenic human papillomavirus (hpv) types is a necessary cause of cervical cancer, the second most frequently occurring cancer in women worldwide. rates of acquisition of hpv are high, particularly among sexually active young adults. reported estimates of incident hpv infection among initially negative women have reached as high as 60% over a 5-year follow-up period. in this article, we review the epidemiology of hpv infection. in addition to estimates of disease frequency, we hi ... | 2005 | 15753008 |
| hpv-mediated transformation of the anogenital tract. | infection with high-risk human papillomavirus (hr-hpv) has been associated with intraepithelial neoplasia and carcinomas at various sites of the anogenital tract, including the cervix, vulva, vagina, penis and anus. although hr-hpv is a necessary cause for cervical cancer, the majority of anal cancers and a subset of cancers at other genital sites, additional (epi)genetic events are required for malignant transformation. hpv-mediated transformation of human epithelial cells has been recognized a ... | 2005 | 15753009 |
| human papillomavirus in squamous cell carcinoma of esophagus in a high-risk population. | to investigate the relation of human papillomavirus (hpv) and esophageal squamous cell carcinoma (escc) in iranian patients as compared to normal controls. | 2005 | 15754405 |
| silencing of integrated human papillomavirus type 18 oncogene transcription in cells expressing serpinb2. | the serine protease inhibitor serpinb2 (pai-2), a major product of differentiating squamous epithelial cells, has recently been shown to bind and protect the retinoblastoma protein (rb) from degradation. in human papillomavirus type 18 (hpv-18)-transformed epithelial cells the expression of the e6 and e7 oncoproteins is controlled by the hpv-18 upstream regulatory region (urr). here we illustrate that pai-2 expression in the hpv-18-transformed cervical carcinoma line hela resulted in the restora ... | 2005 | 15767426 |
| synthesis of gossypol atropisomers and derivatives and evaluation of their anti-proliferative and anti-oxidant activity. | gossypol 1, gossypolone 2, and a series of bis 3 and half schiff's bases 4 of gossypol were synthesised and tested for anti-proliferative and anti-oxidant activity. (-)-gossypol (-)-1 was the most potent inhibitor of the proliferation of the hpv-16 keratinocyte cell line (using an mtt viability assay) with a gi50 of 4.8 microm. the bis schiff's base of (-)-gossypol with l-tyrosine ethyl ester (-)-3b was the most potent inhibitor of iron/ascorbate dependent lipid peroxidation (using the thiobarbi ... | 2005 | 15878283 |
| immune responses induced by lower airway mucosal immunisation with a human papillomavirus type 16 virus-like particle vaccine. | cervical cancer results from cervical infection by human papillomaviruses (hpv), especially hpv16. previous studies have shown that intramuscular vaccination of women with an hpv16 virus-like particle (vlp) vaccine induced a strong igg response and protected against genital hpv16 infection. however, an alternative route of administration that avoids parenteral injection while inducing mucosal immunity might facilitate vaccine implementation in some settings, and partially overcome the substantia ... | 2005 | 15882523 |
| hpv and other risk factors of oral cavity/oropharyngeal cancer in the czech republic. | an association between high-risk human papillomavirus (hr hpv) infection and a risk of development of a subgroup of head and neck cancers has been proposed recently. the main risk factors of oral and oropharyngal cancer observed in our population are smoking and alcohol consumption. the incidence of oral/oropharyngeal tumours in the czech republic is relatively high and there are no data available about the prevalence of hpv dna presence in these tumours. | 2005 | 15888110 |
| presence of high-risk mucosal human papillomavirus genotypes in primary melanoma and in acquired dysplastic melanocytic naevi. | some studies have shown that cutaneous and mucosal melanoma biopsy specimens harbour human papillomavirus (hpv), suggesting that this virus may play a role in development and progression of the tumour. | 2005 | 15888145 |
| human papillomavirus-associated plantar epidermoid cyst related to epidermoid metaplasia of the eccrine duct epithelium: a combined histological, immunohistochemical, dna-dna in situ hybridization and three-dimensional reconstruction analysis. | we recently proposed that certain palmoplantar epidermoid cysts may be related to eccrine ducts and that human papillomavirus (hpv) 60 may play a role in their pathomechanism. however, the origin of palmoplantar epidermoid cysts is still controversial. | 2005 | 15888153 |
| socioeconomic status and the risk of cervical intraepithelial neoplasia grade 3 among oncogenic human papillomavirus dna-positive women with equivocal or mildly abnormal cytology. | low socioeconomic status (ses) is a reported risk factor for cervical carcinoma, but few studies have taken into account adequately the possibly confounding effects of oncogenic human papillomavirus (hpv) infection as well as access to screening and subsequent treatment. | 2005 | 15889450 |
| kinetic analysis of the interactions of human papillomavirus e6 oncoproteins with the ubiquitin ligase e6ap using surface plasmon resonance. | cervical cancers evolve from lesions generated by genital human papillomaviruses (hpv). "low-risk" genital hpvs cause benign proliferations whereas "high-risk" types have the potential to progress into cancer. high-risk hpv e6 oncoproteins interact with the ubiquitin ligase e6ap and target several cellular proteins, including p53 and proteins of the magi family, towards ubiquitin-mediated degradation. e6ap, like other e6 binding proteins such as e6bp, irf-3 and paxillin, interacts with e6 via a ... | 2005 | 15890204 |
| role of the e1--e4 protein in the differentiation-dependent life cycle of human papillomavirus type 31. | the most highly expressed protein in the productive life cycle of human papillomaviruses (hpvs) is e1--e4, but its function is not well understood. to investigate the role of e1--e4, we undertook a genetic analysis in the context of the complete hpv type 31 (hpv31) genome. a mutant hpv31 genome (e4m9) was constructed that contained a stop codon in the e4 open reading frame at amino acid 9 and was silent in the overlapping e2 coding sequence. wild-type and mutant genomes were transfected into nor ... | 2005 | 15890911 |
| papillomavirus capsid mutation to escape dendritic cell-dependent innate immunity in cervical cancer. | infection with oncogenic human papillomaviruses (hpvs), typified by hpv type 16 (hpv16), is a necessary cause of cervical cancer. prophylactic vaccination with hpv16 l1 virus-like particles (vlps) provides immunity. hpv16 vlps activate dendritic cells and a potent neutralizing immunoglobulin g (igg) response, yet many cervical cancer patients fail to generate detectable vlp-specific igg. therefore, we examined the role of the innate recognition of hpv16 l1 in vlp-induced immune responses and its ... | 2005 | 15890912 |
| diversifying selection in human papillomavirus type 16 lineages based on complete genome analyses. | human papillomavirus type 16 (hpv16) is the primary etiological agent of cervical cancer, the second most common cancer in women worldwide. complete genomes of 12 isolates representing the major lineages of hpv16 were cloned and sequenced from cervicovaginal cells. the sequence variations within the open reading frames (orfs) and noncoding regions were identified and compared with the hpv16r reference sequence. this whole-genome approach gives us unprecedented precision in detailing sequence-lev ... | 2005 | 15890941 |
| protection of beagle dogs from mucosal challenge with canine oral papillomavirus by immunization with recombinant adenoviruses expressing codon-optimized early genes. | replication-deficient adenoviral (rad5) vaccines containing codon-optimized e1, e2, e4, and e7 genes of canine oral papillomavirus (copv) were tested singly or in combination to determine which vaccines could protect against mucosal challenge with copv. in three studies, groups of 4-6 beagle dogs were immunized subcutaneously (s.c.) with 10(11) rad5 at 8-10 weeks and 4-6 weeks prior to challenge with infectious copv particles at multiple oral mucosal sites. control dogs were immunized with equiv ... | 2005 | 15892962 |
| human papillomavirus type and tobacco use as predictors of survival in early stage cervical carcinoma. | molecular and environmental co-factors are known risk factors for cervical cancer. the aim of this study was to define the prognostic significance of hpv 18 and its phylogenetically related viruses and smoking on survival in patients with early stage cervical cancer. | 2005 | 15894364 |
| psychosocial factors and the course of cervical intra-epithelial neoplasia: a prospective study. | to investigate the influence of psychosocial factors on the course of cervical intra-epithelial neoplasia (cin). | 2005 | 15894367 |
| modifiable risk factors associated with clearance of type-specific cervical human papillomavirus infections in a cohort of university students. | previous findings regarding risk factors for human papillomavirus (hpv) persistence, other than viral determinants, identified from prospective cohort studies have been inconsistent in part because study designs have differed with respect to differing hpv detection methods and varying lengths of follow-up time. therefore, the objectives of this study were to continue the search for epidemiologic risk factors of persistent cervical hpv infections and determine what behaviors differed between thos ... | 2005 | 15894665 |
| synchronous invasive squamous cell carcinoma and clear cell adenocarcinoma of the uterine cervix: a different human papillomavirus status. | a multiple primary invasive carcinoma of the cervix is a rare condition and is seldom composed of squamous cell carcinoma and clear cell adenocarcinoma. | 2005 | 15896829 |
| prevalence of types 16 and 33 is increased in high-risk human papillomavirus positive women with cervical intraepithelial neoplasia grade 2 or worse. | high-risk human papillomavirus (hrhpv) types are causally related to cervical cancer and its high-grade precursor lesions. the risk posed by the different hrhpv types for the development of cervical intraepithelial neoplasia grade 2 or worse (> or =cin2) needs to be established. here, we present the hrhpv type-distribution in relation to cytology and histology for women participating in a cervical screening program. from 44,102 women who participated in a population-based cervical screening prog ... | 2005 | 15900579 |
| human papillomavirus genotypes and the p53 codon 72 polymorphism in cervical cancer of northeastern thailand. | human papillomavirus (hpv) infection including sub-strain identification was studied in patients with squamous cell cervical cancer (scca) in northeastern thailand. subjects were 90 cases of scca and 100 healthy controls. prevalence of high-risk group of hpv infection in the controls and the scca patients were 13.0% and 86.7%, respectively. the hpv infection significantly increased the risk for cervical cancer 43.5-fold (95% confidential interval: 17.5-110.6; p <0.00001). among hpv carrier patie ... | 2005 | 15905603 |
| comparison between in situ hybridization and real-time pcr technique as a means of detecting the integrated form of human papillomavirus 16 in cervical neoplasia. | integration of the human papillomavirus (hpv) genome is thought to be one of the causes of cancer progression. however, there is controversy concerning the physical status of hpv 16 in premalignant cervical lesions, and there have been no reports on the concordance between detection of the integrated form of hpv16 by real-time pcr and by in situ hybridization. we investigated specimens of cervical intraepithelial neoplasia (cin) and invasive carcinomas for the physical status of hpv 16 by real-t ... | 2005 | 15905694 |
| methylation of human papillomavirus genomes in cells of anal epithelia of hiv-infected men. | intra-anal malignancies disproportionately affect individuals who engage in anal intercourse because of infection with human papillomaviruses (hpvs), with an increased risk attributed to infection with hiv because of a declining immunity against hpvs. long-term persistence of hpvs suggests yet other mechanisms that determine the clinical outcome, however. because methylation of hpv dna represses oncogene expression in cervical samples, we investigated whether this mechanism also occurs in hiv-po ... | 2005 | 15905729 |
| disruption of p53 by the viral oncoprotein hpv16-e6 does not deregulate chromosomal homologous recombination in a transcriptional interference-free assay system. | in addition to its well established role in the maintenance of genome integrity by regulating transcription of genes involved in cell cycle arrest and programmed cell death, the tumour suppressor p53 has also been shown to inhibit spontaneous chromosomal homologous recombination (hr) between adjacent transcription units, raising the possibility that p53 may prevent chromosomal rearrangements by suppressing hr between repetitive dna elements (ectopic hr). consistent with its role in the maintenan ... | 2005 | 15908792 |
| human papilloma virus and breast cancer. | 2005 | 15911436 | |
| the role of diet and nutrition in cervical carcinogenesis: a review of recent evidence. | our objective was to provide an update on recent epidemiologic evidence about the role of diet and nutrition on the risk of human papillomavirus (hpv) persistence and cervical neoplasia, taking hpv into account. we conducted a systematic review and qualitative classification of all observational studies controlling for hpv infection published between march 1995 and november 2003 and of all randomized clinical trials published between january 1991 and november 2003. scientific evidence was classi ... | 2005 | 15912536 |
| [immunomodulatory treatment with beta-interferon in patients with cervical intraepithelial neoplasia and human papillomavirus infection: long-term follow-up]. | the aim of this study was to evaluate the long-term efficacy of beta-interferon treatment in the management of cervical intraepithelial neoplasia associated with human papillomavirus (hpv) infection in a selected group of patients. thirty-nine patients who had histologically proven cervical intraepithelial neoplasia i or ii with concurrent hpv infection were administered 27,000,000 iu of intramuscular beta-interferon. periodical controls were carried out based on papanicolaou smears and/or colpo ... | 2005 | 15915229 |
| preliminary evaluation of a cervical self-sampling device with liquid-based cytology and multiparameter molecular testing. | to compare the collection of a liquid-based cell sample using a cervical self-sampling device and traditional sampling using cytologic and molecular-based tests. | 2005 | 15916209 |
| immortalization of human extravillous cytotrophoblasts by human papilloma virus gene e6e7: sequential cytogenetic and molecular genetic characterization. | extravillous cytotrophoblast (evct) cultures from the normal placentas of three pregnant women were transfected by hpve6e7. sequential cytogenetic and molecular analyses were performed to delineate genetic events that may be critical for cell immortalization. one line, pe1-e6e7, was immortalized successfully, whereas 2 other lines, pe3-e6e7 and pe4-e6e7, could not be maintained beyond crisis. before crisis, the majority of cells in all lines were karyotypically normal. during the early stages of ... | 2005 | 16271953 |
| two different global gene expression profiles in cancer cell lines established from etiologically different oral carcinomas. | cdna arrays were used to characterize the gene expression profiles in 6 oral carcinoma cell lines (ut-scc-10, ut-scc-14, ut-scc-37, ut-scc-54a and ut-scc-54b, ut-scc-74) established from 5 patients with different etiological backgrounds, including young patients, classical risk factors and lichen-derived lesions. in addition, 2 human papillomavirus (hpv)-positive cell lines (hypophraryngeal cancer and hpv16 e6/e7-transformed oral keratinocytes) were similarly tested. two distinct global gene exp ... | 2005 | 16273247 |
| analysis of tumor progression by transcriptional profiling of mouse mk16 cell lines transformed with human papillomavirus type 16 e6 and e7 oncogenes and activated h-ras. | a better understanding of the molecular basis of tumor progression and invasion is needed to improve therapy for malignant tumors. recently, we established a mouse metastatic mk16 model by transduction of secondary kidney cells with human papillomavirus type 16 (hpv16) e6 and e7 oncogenes and human h-ras activated by g12v mutation. in this study, we extended the model to mk16 cell lines derived from lung metastases and compared the oncogenicity of seven cell lines successively isolated from prim ... | 2005 | 16273273 |
| sporadic case of warts, hypogammaglobulinemia, immunodeficiency, and myelokathexis syndrome. | the term whim syndrome (whims) is an acronym describing a rare primary immunodeficiency disorder characterized by warts, hypogammaglobulinemia, immunodeficiency, and myelokathexis, the unusual association of neutropenia with bone marrow myeloid hypercellularity. whims was recently associated with mutations in the gene encoding the chemokine receptor cxcr4 and as such is the first disease ascribed to abnormalities of chemokine signaling. we report a sporadic case of whims in a woman presenting wi ... | 2005 | 16275383 |
| a population-based analysis of temporal trends in the incidence of squamous anal canal cancer in relation to the hiv epidemic. | squamous cell carcinoma of the anal canal (scca) is etiologically linked to human papillomavirus, and its incidence is increased among the immunosuppressed. we used data from the surveillance, epidemiology, and end results program to analyze the incidence of scca in relation to 3 separate periods during the hiv epidemic: the pre-hiv era (1973-1981), the hiv era (1982-1995), and the highly active antiretroviral treatment (haart) era (1996-2001). the incidence per 100,000 population of scca increa ... | 2005 | 16280701 |
| interactions with pocket proteins contribute to the role of human papillomavirus type 16 e7 in the papillomavirus life cycle. | human papillomaviruses (hpvs), most commonly the hpv16 genotype, are the principle etiological determinant for cervical cancer, a common cancer worldwide resulting in over 200,000 deaths annually. the oncogenic properties of hpvs are attributable in part to the virally encoded protein e7, best known for its ability to bind to and induce the degradation of the retinoblastoma tumor suppressor, prb, and related "pocket proteins" p107 and p130. previously, we defined a role for e7 in the productive ... | 2005 | 16282477 |
| skin hyperproliferation and susceptibility to chemical carcinogenesis in transgenic mice expressing e6 and e7 of human papillomavirus type 38. | the oncoproteins e6 and e7 of human papillomavirus type 38 (hpv38) display several transforming activities in vitro, including immortalization of primary human keratinocytes. to evaluate the oncogenic activities of the viral proteins in an in vivo model, we generated transgenic mice expressing hpv38 e6 and e7 under the control of the bovine homologue of the human keratin 10 (k10) promoter. two distinct lines of hpv38 e6/e7-expressing transgenic mice that express the viral genes at different leve ... | 2005 | 16282489 |
| awareness of general and personal risk factors for uterine cancer among healthy women. | participation rates in gynaecological cancer screening are influenced by different factors. the knowledge of general and personal risk factors for uterine cancer among women might influence their interest in gynaecological cancer screening. two thousand nine hundred women in 23 gynaecological outpatient services were invited to answer a structured questionnaire regarding general and personal risk factors for cervical and endometrial carcinoma; 2108 women participated. women with a history of can ... | 2005 | 16284496 |
| requirement of epidermal growth factor receptor for hyperplasia induced by e5, a high-risk human papillomavirus oncogene. | multicellular organisms rely on complex networks of signaling cascades for development, homeostasis, and responses to the environment. these networks involve diffusible signaling molecules, their receptors, and a variety of downstream effectors. alterations in the expression or function of any one of these factors can contribute to disease, including cancer. many viruses have been implicated in cancer, and some of these modulate cellular signal transduction cascades to carry out their life cycle ... | 2005 | 16061632 |
| association of the human papillomavirus type 16 e7 oncoprotein with the 600-kda retinoblastoma protein-associated factor, p600. | the human papillomavirus type 16 (hpv-16) e7 gene encodes a multifunctional oncoprotein that can subvert multiple cellular regulatory pathways. the best-known cellular targets of the hpv-16 e7 oncoprotein are the retinoblastoma tumor suppressor protein prb and the related pocket proteins p107 and p130. however, there is ample evidence that e7 has additional cellular targets that contribute to its transforming potential. we isolated hpv-16 e7 associated cellular protein complexes by tandem affini ... | 2005 | 16061792 |
| anal cytology: is there a role for reflex hpv dna testing? | there is an increased incidence of anal squamous carcinoma and its precursor lesions (anal intraepithelial neoplasia [ain]) among persons who engage in anal-receptive sex. analogous to cervical cancer screening, anal papanicplaou (pap) smears currently are used to screen these high-risk populations. human papilloma virus (hpv) has been implicated in anal carcinoma pathogenesis and this study was performed to assess the potential role of hpv dna testing as an adjunct to anal cytology. we correlat ... | 2005 | 16078257 |
| concordance of specific human papillomavirus types in sex partners is more prevalent than would be expected by chance and is associated with increased viral loads. | genital human papillomavirus (hpv) infections are generally accepted to be sexually transmitted, but studies of hpv infections in sex partners are limited. we investigated hpv type-specific concordance and viral load in 238 heterosexual couples. women with cervical intraepithelial neoplasia were the index patients in these couples. | 2005 | 16080082 |
| concordance of human papillomavirus (hpv) type between sex partners: implications for effective utilization of an hpv vaccine. | 2005 | 16080083 | |
| viral oncoprotein-induced mislocalization of select pdz proteins disrupts tight junctions and causes polarity defects in epithelial cells. | the development of human cancers is frequently associated with a failure of epithelial cells to form tight junctions and to establish proper apicobasal polarity. interestingly, the oncogenic potential of the adenovirus e4-orf1 protein correlates with its binding to the cellular pdz proteins mupp1, magi-1, zo-2 and sap97, the first three of which assemble protein complexes at tight junctions. given that e4-orf1 sequesters these three pdz proteins in the cytoplasm of fibroblasts, we postulated tha ... | 2005 | 16141229 |
| human papillomavirus vaccines and adolescents. | this review will describe human papillomavirus (hpv) vaccines in development, summarize data regarding safety and efficacy of these vaccines, and discuss key issues related to hpv vaccine implementation. | 2005 | 16141761 |
| [usefulness of searching for human papillomavirus (hpv): evaluation of screening practices for precancerous lesions of the uterine cervix]. | 2005 | 16142172 | |
| dna vaccines based on chimeric potyvirus-like particles carrying hpv16 e7 peptide (aa 44-60). | vaccine strategies for the treatment of human papillomavirus-induced cervical cancer are based mainly on the human papillomavirus 16 e7 (hpv16 e7) oncoprotein. the immunogenicity of the e7 gene has been enhanced by its fusion to many different genes. here, we linked a short sequence coding for the e7 peptide (aa 44-60) containing immunodominant epitopes for b and t cells to the 3' end of the gene coding for the whole coat protein (cp) of the poty-virus, potato virus a (pva), and its deleted form ... | 2005 | 16142370 |
| the expression of p53 protein and infection of human papilloma virus in conjunctival and eyelid neoplasms. | human papilloma virus (hpv) infection and loss of p53 function have been identified as frequent events in various human tumors. the aim of this study was to evaluate p53 protein expression and to detect hpv in the tissue samples of 45 benign (papillomas) and 38 malignant conjunctival and eyelid lesions (27 basal cell carcinomas and 11 squamous cell carcinomas). we also looked for eventual relationships between p53 expression and clinicopathological features such as age, histological type of tumo ... | 2005 | 16142387 |
| human leukocyte antigen class i and ii haplotypes and risk of cervical cancer. | human leukocyte antigen (hla) variations may affect immune response to human papillomavirus infection and subsequent cervical neoplasia risk. we investigated the frequency and relationship between hla-a-b and hla-a-b-dr haplotypes among women with cervical cancer/high-grade lesions (n=365) and cytologically normal population controls (n=681) within three cervical neoplasia studies in the us and costa rica. notable differences in haplotype frequencies were observed; the hla-a*01-b*08 haplotype oc ... | 2005 | 16185329 |
| prevalence of human papillomavirus cervical infection in an italian asymptomatic population. | in the last decade many studies have definitely shown that human papillomaviruses (hpvs) are the major cause of cervical carcinogenesis and, in the last few years, hpv testing has been proposed as a new and more powerful tool for cervical cancer screening. this issue is now receiving considerable attention in scientific and non scientific press and hpv testing could be considered the most important change in this field since the introduction of cervical cytology. this paper reports our prevalenc ... | 2005 | 16188026 |
| verrucous carcinoma of the scalp associated with human papillomavirus type 33. | verrucous carcinoma (vc) is a low-grade, well-differentiated squamous cell carcinoma of the skin or mucosae, and human papillomavirus (hpv) infection has been considered to be one of the causative factors of vc at three main sites, including the oral cavity, the genitoanal region, and the foot. however, the relationship between cutaneous vc at other sites and hpv infection remains obscure. | 2005 | 16188198 |
| routine cervical screening with primary hpv testing and cytology triage protocol in a randomised setting. | the role of high-risk human papillomavirus (hrhpv) testing in primary cervical screening has not been established. we generated a randomised evaluation design ultimately to clarify whether primary hrhpv testing implemented into routine screening can bring increase in the programme effectiveness. the aim of the present report on first-year results was to assess the cross-sectional relative validity parameters for routine hrhpv screening, in comparison with conventional screening. an equal number ... | 2005 | 16189520 |
| human papillomavirus type 26-associated periungual squamous cell carcinoma in situ in a hiv-infected patient with concomitant penile and anal intraepithelial neoplasia. | 2005 | 16198810 | |
| characterization of hpv-16 e6 dna vaccines employing intracellular targeting and intercellular spreading strategies. | human papillomavirus (hpv) e6 and e7 are consistently expressed and are responsible for the malignant transformation of hpv-associated lesions. thus, e6 and e7 represent ideal targets for therapeutic hpv vaccine development. we have previously used the gene gun approach to test several intracellular targeting and intercellular spreading strategies targeting hpv-16 e7. these strategies include the use of the sorting signal of lysosome-associated membrane protein (lamp-1), mycobacterium tuberculos ... | 2005 | 16200349 |
| reproducibility of hpv dna testing by hybrid capture 2 in a screening setting. | within a large italian randomized trial on new technologies for cervical cancer screening involving 7 laboratories with different levels of experience, an intralaboratory and interlaboratory quality control program for human papillomavirus (hpv) dna testing by hybrid capture 2 (hc2; digene, gaithersburg, md) was implemented. to monitor the hybridization and detection steps, target samples containing purified, concentration-defined, hpv dna were introduced in each test run. only 3 of 1,024 showed ... | 2005 | 16203283 |
| human papillomavirus infections of the genital and respiratory tracts in young children. | human papillomavirus (hpv) causes papillomas (warts) on the skin and respiratory mucosal surfaces (laryngeal and oral papillomas) in addition to condyloma acuminata (anogenital warts). hpv has become one of the most common sexually transmitted diseases in adults. vertical transmission from mother to infant during birth is well recognized. laryngeal papillomas are the most common tumors of the larynx in children worldwide, and recurrent lesions are common occurrences. anogenital warts in children ... | 2005 | 16210110 |
| identification of human papillomavirus 16-e6 protein-derived peptides with the potential to generate cytotoxic t-lymphocytes toward human leukocyte antigen-a24+ cervical cancer. | human papillomavirus 16 (hpv16)-e6 and -e7 proteins are considered to be appropriate targets in specific immunotherapy for cervical cancer. in this study, we attempted to identify epitope peptides from the hpv16-e6 protein that have the potential to generate cytotoxic t-lymphocytes (ctls) toward human leukocyte antigen (hla)-a24+ cervical cancer. two hpv16-e6 peptides at positions 75-83 and 91-100 effectively induced peptide-specific ctls from peripheral blood mononuclear cells of hla-a24+ cervi ... | 2005 | 16211234 |
| prognostic value of human papillomavirus in the survival of head and neck cancer patients: an overview of the evidence. | despite improvements over the last decade, survival rates for head and neck squamous cell carcinoma (hnscc) remain around 50% and variable. recently, a subset of hnscc has been shown to contain human papillomavirus (hpv) and the presence of hpv in tumors constitutes a prognostic marker of disease. hpv-dna in these tumors (overwhelmingly genotype 16, a high-risk type also found in cervical cancer) is present at high copy numbers, frequently integrated, and often transcriptionally active. in compa ... | 2005 | 16211291 |
| a bi-functional sirna construct induces rna interference and also primes pcr amplification for its own quantification. | rna interference (rnai) is a process of post-transcriptional gene silencing initiated by double-stranded rnas, including short interfering rna (sirna). silencing is sequence-specific and rnai has rapidly become central to the study of gene function. rnai also carries promise for selective silencing of viral and endogenous genes causal for disease. to detect the very low levels of sirna effective for rnai we modified the 3' end of the sense strand of sirna with a nuclease-resistant dna hairpin. w ... | 2005 | 16214804 |
| esophageal squamous papillomatosis. | esophageal squamous papillomatosis is rare and has been associated with gastroesophageal reflux and recurrent respiratory papillomatosis. we report a case of extensive esophageal papillomatosis, no airway involvement and a slowly progressive clinical course with progressive strictures and ultimately fatal squamous cell carcinoma. in-situ hybridization performed on biopsy specimens was negative for high-risk human papilloma virus types. due to the paucity of reported cases, little is conclusively ... | 2005 | 16215437 |
| structural and energetic origins of sequence-specific dna bending: monte carlo simulations of papillomavirus e2-dna binding sites. | dna bending is an important structural feature for indirect readout in protein-dna recognition. the binding of papillomavirus e2 transcription factors to their dna binding sites is associated with dna bending, providing an attractive model system to study the origins of sequence-specific dna bending. the consensus e2 target is of the general form accgn(4)cggt with a variable four base pair region. we applied a new all-atom monte carlo (mc) algorithm that combines effective sampling with fast con ... | 2005 | 16216581 |
| primary prevention of human papillomavirus-dependent neoplasia: no condom, no sex. | cervix cancer is one of several neoplastic disorders that arise following transfer of human papillomavirus (hpv) during unprotected sexual intercourse, and like most other sexually transmitted diseases (stds), is largely preventable by consistent condom use. this primary prevention strategy has received little support, however, when compared with massive secondary prevention initiatives involving cervical screening. the reasons for this anomalous situation are complex, and include: (i) the asymp ... | 2005 | 16223580 |
| sexually-transmitted viral diseases in women: clinical and epidemiological aspects and advances in laboratory diagnosis. | sexually transmitted diseases (stds) have long been known, but they have only recently been recognized as causes of significant long-term morbidity, mainly as a result of increased knowledge concerning viral stds. the relationship of these diseases with conditions such as anogenital cancer and acquired immunodeficiency syndrome (aids) has made viral stds an important issue in the healthcare of women and infants, and in reproductive health. the evolution of the aids pandemic is now characterized ... | 2005 | 16224631 |
| length of human immunodeficiency virus disease and not immune status is a risk factor for development of anal carcinoma. | the anal epithelium is subject to dysplastic change in patients with human immunodeficiency virus (hiv). we sought to determine if the duration of hiv disease or the patient's immune status were associated with the development of anal carcinoma. | 2005 | 16226949 |
| coming to terms with vietnam: the viet/american cervical cancer prevention project. | the viet/american cervical cancer prevention project embraces a dual mission. we seek to develop sustainable, cost-effective cervical cancer prevention services for women in vietnam. because the problem of cervical cancer in vietnam is in part a legacy of the second indochinese war, we also seek to examine obstacles to reconciliation by presenting what most acknowledge to be a remedy in advance of what some will perceive to be an accusation. certain research and commercial interests have produce ... | 2005 | 16240403 |
| identification of hpv variants. | the vast majority of anogenital carcinomas are caused by high-risk human papillomaviruses (hpvs), and among western nations hpv-16 is usually the most predominant cancer-associated type. as a dna virus, hpv type 16 has a relatively stable genome that is believed to have co-evolved with its host over the millennia. nevertheless, among the "wild" populations of hpv-16 that are circulating, a large number of variants have been identified, and these may have considerably different pathogenic potenti ... | 2005 | 16350393 |
| histological analysis of cervical intraepithelial neoplasia. | a wide interobserver variation is seen even among competent histopathologists in the routine diagnosis of cervical intraepithelial neoplasia (cin). as a result, early detection of low-grade cin (cin 1) lesions, in particular, remains a major challenge both in routine diagnosis and in cervical screening. in this chapter, the salient diagnostic features of human papillomavirus infection and cin lesions are demonstrated. | 2005 | 16350395 |
| analysis of p16ink4a and integrated hpv genomes as progression markers. | most cervical cancers are preventable when the precursor lesions are detected in time. human papilloma viruses (hpvs) are the main risk factors for cervical cancer development, but there is a high percentage of healthy women infected with hpv that never develop a lesion. only a small percentage of low-grade dysplasias finally grow out to invasive cancer. several biomarkers can be used to identify lesions at risk for malignant progression. overexpression of p16ink4a is induced by the viral oncopr ... | 2005 | 16350398 |
| hpv dna detection and typing in inapparent cutaneous infections and premalignant lesions. | epidemiological studies, which address the role of human papillomavirus (hpv) in the pathogenesis of (pre)malignant cutaneous lesions, focus on the hpv b1 subgroup comprising the so-called epidermodysplasia verruciformis (ev)-associated hpv types. to detect and type hpv dna in human materials, polymerase chain reaction (pcr)-based assays are used. in this chapter, a nested, broad-spectrum pcr method using a mixture of primers and a type-specific pcr using specific primers are described. the broa ... | 2005 | 16350401 |
| propagation of infectious, high-risk hpv in organotypic "raft" culture. | the organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (hpv) life cycle, including virion morphogenesis. this system utilizes hpv-containing cell lines that are either derived from biopsies or created by the transfection of keratinocytes with hpv genomic dna. when grown as raft cultures, these lines allow for a detailed study of all stages of the viral life cycle. in this chapter, we describe in detail how ... | 2005 | 16350404 |
| analysis of adeno-associated virus and hpv interaction. | it is slowly becoming accepted that adeno-associated virus (aav) is another significant factor involved in cervical carcinogenesis. however, unlike human papillomavirus (hpv), which is positively associated with cervical cancer, aav is negatively associated with this cancer. this negative association appears to be through a direct and complex bi-directional interaction between aav and hpv. essentially all assays used for studying hpv can be used for studying the aav-hpv interaction. this is beca ... | 2005 | 16350413 |
| codon optimization of papillomavirus genes. | early and late genes of human and animal papillomaviruses show a codon composition seemingly unfavorable for expression in mammalian cells. it remains unclear how the viruses manage to achieve high levels of late gene expression during the viral life cycle. one possible solution could be that the availability of certain t-rnas changes with progressing stages of cellular differentiation. previous studies have demonstrated that modification of codon usage of papillomavirus late (l1 and l2) and ear ... | 2005 | 16350416 |
| generation and applications of hpv pseudovirions using vaccinia virus. | this chapter outlines the generation and application of human papillomavirus type 33 (hpv 33) pseudovirions. these pseudovirions are structurally indistinguishable from native virions and are therefore valuable tools for the study of papillomavirus/cell interactions. the method describes (1) the construction of vaccinia viruses recombinant for the major and minor hpv capsid proteins, l1 and l2, respectively, (2) the transfection of cos7 cells with a marker plasmid replicating to high copy number ... | 2005 | 16350418 |
| the hpv xenograft severe combined immunodeficiency mouse model. | the permissive propagation of papillomaviruses outside their natural hosts has not been possible, which is an important restriction to the study of human papillomavirus (hpv) infections and associated diseases. since the mid-1980s, several models have been described that rely on the growth of hpv in susceptible human xenografts implanted in immunodeficient mice. the severe combined immunodeficiency (scid) mouse has been particularly suited to this approach, and to reproduce reliably the macrosco ... | 2005 | 16353337 |
| [efficacy and safety of 5% imiquimod cream in external genital warts: a 6 month follow-up evaluation]. | one of the main issues raised by the management of external genital warts is their potential risk of recurrence. however, nearly all studies assessing recurrences have been conducted with a 3 month follow-up. since the latency of the human papillomavirus is long, such 3 month follow-up might be too short to detect the true recurrence rate. we therefore conducted a study evaluating patients with external genital warts, treated with an immune response modifier, 5% imiquimod cream, and followed up ... | 2005 | 16327713 |
| a theoretical discussion of the impact of stigma on psychological adjustment to having a sexually transmissible infection. | this paper provides a discussion of the utility of stigma theory as a conceptual framework for the interpretation and analysis of the psychological impact of contracting a sexually transmissible infection (sti). most particularly, it focuses on those viral infections that cannot be medically cured, such as genital herpes and genital human papilloma virus. recent research in the area of stis suggests that the stigma associated with these conditions can hinder psychosocial and sexual adjustment po ... | 2005 | 16335743 |
| human papillomavirus: burden of illness and treatment cost considerations. | the monetary and personal costs to society of human papillomavirus (hpv) infection are enormous. in order to make a comparison of different treatment methods we reviewed the entire literature on hpv treatment from january 1966 to december 2003 using medline, with particular reference to published meta-analyses, randomized controlled and comparative studies. patient-applied therapies offer patients the possibility of convenient and, on the whole, pain-free treatment. podofilox (podophyllotoxin) a ... | 2005 | 16343025 |
| high prevalence of human papillomavirus infections in urine samples from human immunodeficiency virus-infected men. | infection with human immunodeficiency virus (hiv) and the resulting immunosuppression are associated with an increased risk for human papillomavirus (hpv) persistence and related malignancies. in the present study we investigated the prevalence of hpv in urine samples from 104 hiv-infected men with low cd4+ cell counts (<100 per mm(3)) and 115 urine samples from hiv-negative men. a high prevalence of hpv dna (39.4%) was found in the hiv patients. most of the hpv types were high risk (81.4%), wit ... | 2005 | 16333078 |