Publications

recombinational bypass of pyrimidine dimers promoted by the reca protein of escherichia coli.	reca protein, in the presence of single-stranded dna binding protein and atp, promotes the complete exchange of strands between circular single-stranded dna containing pyrimidine dimers and a homologous linear duplex, converting the pyrimidine dimer-containing single-stranded dna to a circular duplex. bypass of a pyrimidine dimer during the branch-migration phase of the reaction requires approximately 20 seconds, a rate 1/50th of that in the absence of the dimer. the circular duplex product is s ...	1982	6954468
alpha-helix dipole model and electrostatic stabilization of 4-alpha-helical proteins.	a simple dipole model is developed for estimation of the electrostatic interaction energy between alpha-helices in proteins. this model is used to estimate the electrostatic stabilization in a recurrent protein tertiary structural motif, an array of four closely packed alpha-helices. it is found that, for the proteins examined (cytochrome c', hemerythrin, myohemerythrin, cytochrome b562, and a t4 phage lysozyme domain), their common antiparallel arrangement of adjacent helices confers a stabiliz ...	1982	6956878
kinetic evidence suggesting two mechanisms for iodothyronine 5'-deiodination in rat cerebral cortex.	enzymatic 5'-deiodination of 3,3',5'-triiodothyronine (rt3) and 3,3',5,5'-tetraiodothyronine (thyroxine, t4) was studied in microsomal preparations of rat cerebral cortex. evidence was obtained for the existence of two thiol-dependent 5'-deiodinase entities. one of these predominates in tissue from euthyroid and long-term hypothyroid rats, is specific for rt3, follows "ping-pong" kinetics with dithiothreitol as the cosubstrate, and is inhibited by propylthiouracil (prsura) and iodoacetate. inhib ...	1982	6956917
glucocorticoid-binding proteins in rat liver.	the distribution, metabolism and protein-binding of [3h] corticosterone in the rat liver was studied with respect to time and sex. the maximum recovery from the cell nuclei occurred 5 min after injection of the steroid. at this time, ten times more radioactivity was recovered from the liver cell nuclei of male animals compared to females. the recovered radioactivity in the cell nuclei was identified as mainly unmetabolised corticosterone and 5 alpha-dihydrocorticosterone. the radioactivity bound ...	1982	6958493
[recombination in bacteriophage t4: the nature of the disturbances introduced into genetic recombination by a region of non-homology].	effects of a non-homology region associated with a deletion marker on recombination were studied in two-factor crosses between rii mutants of t4 phage. the basic experimental approach consisted of the comparison of a deletion with the point mutation located at one of the borders of the segment deleted in crosses against the same set of the test markers. the results obtained are in good accordance with the predictions of the model of elastic interaction of migrating cross connection with the nonh ...	1982	6958635
serum gastrin and motilin in treated and untreated hyperthyroidism.	to study the gastrointestinal hormones in hyperthyroidism and the interrelations with the thyroid hormones, the preprandial serum gastrin and motilin were measured by radioimmunoassay in 104 patients before and after the treatments. the following results were obtained: 1) serum gastrin concentration in hyperthyroidism was significantly higher than that of the control, and when the patients reached euthyroidism or hypothyroidism after the treatments, the gastrin concentrations in the serum decrea ...	1982	6959740
dna primase activity associated with dna polymerase alpha from xenopus laevis ovaries.	one of the two forms of dna polymerase alpha from ovaries of the frog xenopus laevis catalyzed ribonucleoside triphosphate-dependent dna synthesis on single-stranded circular fd phage dna templates. dna synthesis was dependent on atp and added template. ctp, gtp, and utp stimulated dna synthesis but were not required and could not substitute for atp. dna synthesis was not inhibited by alpha-amanitin. neither poly(dt) nor double-stranded dna served as template. analysis of [32p]-dtmp-labeled prod ...	1982	6961403
acquisition of immune competence by a subset of human cortical thymocytes expressing mature t cell antigens.	previous studies indicated that the human thymus is composed of several discrete compartments. cortical thymocytes are reactive with a monoclonal antibody, anti-t6 (tl-like) and coexpress antigens defined by antibodies anti-t4 and anti-t8. in contrast, most medullary thymocytes are unreactive with anti-t6, express either t4 or t8 antigens, and more importantly, brightly stain with anti-t3 and anti-t1, which define mature t cell antigens. only a minor population of cortical thymocytes strongly ex ...	1982	6980916
the role of t cell differentiation markers in antigen-specific and lectin-dependent cellular cytotoxicity mediated by t8+ and t4+ human cytotoxic t cell clones directed at class i and class ii mhc antigens.		1982	6980945
characterization of hnk-1+ (leu-7) human lymphocytes. i. two distinct phenotypes of human nk cells with different cytotoxic capability.	human lymphocytes with nk and k cell activities can be identified by the hnk-1 (leu-7) monoclonal antibody. in these experiments, subsets of hnk-1+ cells from blood and bone marrow were distinguished by their expression of other cell surface antigens, their morphology, and their nk functional capability. two-color immunofluorescence analysis revealed that subpopulations of hnk-1+ cells in blood expressed antigens found on mature t cells (e.g., t1, t3, t4, t8), but none expressed antigens charact ...	1982	6980948
distribution of t-cell subsets identified by monoclonal antibodies in cell suspensions from lymph node biopsies of human b-cell lymphomas.	the distribution of t-cell subsets has been examined in cell suspensions from lymph node biopsies from 37 non-hodgkin lymphomas (nhl) of b-cell origin and 8 cases of hodgkin's disease (hd). t-inducer/helper cells (t4+ cells) and t-suppressor/cytotoxic cells (t8+ cells) were identified by the monoclonal antibodies okt4 and okt8, respectively. compared with reactive lymph nodes the t-cell subset distribution was aberrant in 18/37 (48.6%) of the b-cell lymphomas. the t-suppressor subset was dominat ...	1982	6981200
purification of bacteriophage t4 gene 61 protein. a protein essential for synthesis of rna primers in the t4 in vitro dna replication system.	an activity dependent on an intact bacteriophage t4 gene 61 is required, along with the t4 gene 41 protein, for the synthesis of ribonucleotide primers in an in vitro t4 dna replication system. in this paper, we present a method for purification of the protein catalyzing this gene 61-dependent activity based on an assay for primer-dependent dna synthesis by the t4 dna replication proteins. the t4 gene 32 helix-destabilizing protein influences the chromatographic behavior of 61 protein. the purif ...	1982	6981646
bacteriophage t4 gene 44/62 and gene 45 polymerase accessory proteins stimulate hydrolysis of duplex dna by t4 dna polymerase.		1982	6981648
in situ localization of lymphocyte subsets in synovial membranes of patients with rheumatoid arthritis with monoclonal antibodies.	immunological analysis of the mononuclear infiltrates in 12 rheumatoid synovial membranes was performed by means of modified peroxidase antiperoxidase technique using a panel of monoclonal antibodies, directed against t cell differentiation antigens and hla-dr (ia-like) antigens. helper/inducer t lymphocytes (okt4 +, leu3a+) were found in large numbers in nodular lymphoplasmocellular infiltrates, whereas the number of cytotoxic/suppressor t lymphocytes (okt8 +m leu2a+) was very low, resulting in ...	1982	6981702
clonal analysis of human cytotoxic t lymphocytes: t4+ and t8+ effector t cells recognize products of different major histocompatibility complex regions.	alloreactive human t lymphocytes were cloned in soft agar or by limiting dilution and subsequently propagated with interleukin 2 and alloantigen for 8 months or more. by indirect immunofluorescence every clone was reactive with anti-ia antibodies as well as the t cell-specific antibodies anti-t3 and anti-t11 and expressed either t4 or t8 antigens. all 15t8+ clones were highly cytotoxic for the sensitizing alloantigen. in contrast, only two of seven t4+ clones mediated cytotoxic effector function ...	1982	6981813
surface structures involved in target recognition by human cytotoxic t lymphocytes.	cloned human cytotoxic t lymphocytes and monoclonal antibodies inhibiting their function (anti-t3a, anti-t4a, and anti-t8a) were used to elucidate the role of t cell surface glycoproteins in cell-mediated lympholysis involving individual classes of gene products of the major histocompatibility complex on target cells. the results indicate that several surface molecules are required for specific target recognition: t3 and t4 on t4+ cytotoxic t lymphocytes and t3 and t8 on t8+ cytotoxic t lymphocy ...	1982	6981845
antigen recognition by human t lymphocytes is linked to surface expression of the t3 molecular complex.	four distinct surface molecules on human t cells are defined by the monoclonal antibodies anti-t1, anti-t3 (anti-t3a), anti-t11 and anti-t12. following cell binding, anti-t3 (anti-t3a) and anti-t1 induce independent modulation of their respective ligands, whereas anti-t11 and anti-t12 do not. to explore the biological consequences of this modulation, we used cloned populations of t4 and t8 cytotoxic t lymphocytes. anti-t3 (anti-t3a), but not anti-t1, inhibits cytotoxic t lymphocyte effector func ...	1982	6982759
genetic specificity of dna synthesized in the absence of t4 bacteriophage gene 44 protein.	upon infection of escherichia coli b with t4 phage with do amber mutation in gene 44, a minimal amount of phage dna is synthesized. this progeny dna is, for the most part, covalently attached to the parental dna. analysis of the genetic representation of this dna was performed by hybridization to cloned genetic segments. it was shown that areas preferentially replicated differ from origins observed in "normal" replication: under normal conditions, there is a strong origin in the genetic area of ...	1982	6982976
in situ characterization of mononuclear cells in rheumatoid tissues, using monoclonal antibodies. no reduction of t8-positive cells or augmentation in t4-positive cells.	the reactivity of monoclonal mouse anti-human antibodies specific for mononuclear cell surface antigens were studied by the indirect immunofluorescence technique in frozen synovial tissue sections from patients with rheumatoid arthritis (ra). most of the proliferating synovial lining cells were positive for hla-dr antigens and monocyte-specific antigens, since they reacted with the okia1 and okm1. cells positive for hla-dr and monocyte antigens were also seen scattered or in small nests in the s ...	1982	6983117
characterization by monoclonal antibodies of lymphocyte subsets present in b-enriched suspensions.	b lymphocyte enriched suspensions isolated by e rosette depletion (e-cells) or by nylon fiber adherence (adherent cells) were identified by their cellular composition using different t and b cell markers (sig,e receptor, t3, t4, t8 and ia-like antigens). the cells were isolated from peripheral blood both of healthy donors and uremic dialysis patients. a variable proportion of non-b cells was found in some preparations. this contaminant was represented mainly by null cells in e-lymphocyte suspens ...	1982	6983159
monoclonal anti-t cell antibodies are applicable to the study of inflammatory infiltrates in the central nervous system.	monoclonal anti-human t cell antibodies were tested by a modified pap technique on frozen sections of human central nervous system (cns) tissue from inflammatory and non-inflammatory conditions. it was found that whole t cells and t cell subsets--t4+ (helper-inducer) and t8+ (suppressor/cytotoxic) t cells--could be differentiated specifically from other mononuclear cells and that these markers did not cross-react with human cns tissue elements, particularly oligodendrocytes, under the same condi ...	1982	6983529
autoregulation of gene expression. quantitative evaluation of the expression and function of the bacteriophage t4 gene 32 (single-stranded dna binding) protein system.		1982	6984860
thyroid hormone stimulation in vitro of red blood cell ca2+-atpase activity: interspecies variation.	in vitro susceptibility to thyroid hormone stimulation of membrane-associated ca2+-atpase activity has been examined in red blood cells from rat, rabbit, dog, monkey, and man. monkey and human red cell ca2+-atpase activities responded comparably to 10(-10)m t4 or t3. basal and thyroid hormone-stimulated ca2+-atpase activity in rabbit erythrocytes was four-fold higher than in primate red cells. rat and dog red cell ca2+-atpase did not respond to iodothyronines in vitro.	1982	6459228
decreased suppressor t-lymphocytes in autoimmune thyroid diseases detected by monoclonal antibodies.	monoclonal antibodies reacting with cell surface antigens of helper (t4), suppressor (t8) t cells and common t-cell antigen (t3) were used by an immunofluorescence technique to enumerate peripheral t-lymphocytes in 42 patients with graves' disease and 16 patients with hashimoto's thyroiditis. the percentages of total t cells (cells which react with anti-t3) and helper/inducer cells (cells which react with anti-t4) among peripheral mononuclear cells in graves' and hashimoto's patients were not si ...	1982	6459338
rapid hydrolysis of deoxynucleoside triphosphates accompanies dna synthesis by t4 dna polymerase and t4 accessory proteins 44/62 and 45.		1982	6460024
specific sequences in native dna that arrest synthesis by dna polymerase alpha.	the effect of the dna sequence of a template on the progress of dna polymerase alpha was determined at the resolution of single nucleotides by using single-stranded, circular phi x174 dna as a template and unique phi x 174 ona fragments terminally labeled at their 3'-ends as primers. therefore, the amount of radioactivity observed was always proportional to the number and not the length of nascent dna chains. extension of a primer by alpha-polymerase revealed that 3'-ends of nascent dna chains a ...	1982	6460033
heterogeneity of human t4+ inducer t cells defined by a monoclonal antibody that delineates two functional subpopulations.	a monoclonal antibody termed anti-t4 that detected approximately 60% of peripheral blood t lymphocytes was shown to define the human inducer population. in the present study, we characterized three additional monoclonal antibodies, anti-t4a, anti-t4b, and anti-tq1, that were reactive with a similar percentage of t lymphocytes. anti-t4a, anti-t4b, and anti-t4 delineated identical cell populations, while those defined by anti-tq1 differed in several respects: 1) anti-tq1 stained a minority (less t ...	1982	6459376
communicative interactions between subpopulations of human t lymphocytes required for generation of suppressor effector function in a primary antibody response.	the cellular interactions necessary for generation of human t suppressor effector function were examined in a primary in vitro antigen-specific anti-dnp antibody-forming system. during the induction of the anti-dnp response, it was found that t cells of both t4+ and t8+ subsets were necessary to suppress antibody formation, whereas after activation, only the t8+ subset was required. thus, a population of t4+ t cells appears to be necessary to activate or induce a subset of t8+ cells to suppress. ...	1982	6460807
the characterization and function of human immunoregulatory t lymphocyte subsets.	functional t lymphocyte subpopulations can be identified in humans by antibodies which detect stable glycoprotein antigens on their surface. thus, inducer t lymphocytes bear an antigen termed t4 while suppressor t lymphocytes bear an antigen termed t5. immune homeostasis results from a delicate balance between inducer and suppressor subsets within the t-cell circuit and perturbation in subset dynamics may initiate a wide variety of immunopathological disorders. here ellis reinherz and stuart sch ...	1982	6461862
evidence for mild thyroidal impairment in women undergoing endurance training.	the effects of endurance training on body composition and the pituitary-thyroid axis were studied in 29 healthy, young (mean age, 28.7 yr), regularly menstruating women. women who were initially jogging a mean of 13.5 miles/week were selected for this study to minimize dropouts. body composition, measured by hydrostatic weighing, and nonfasting plasma concentrations of t4, t3, rt3, tsh, and trh-stimulated tsh, measured by ria, were examined initially and after each subject's weekly mileage had i ...	1982	6798059
familial hyperthyroidism due to inappropriate thyrotropin secretion successfully treated with triiodothyronine.	a family of three generations is described in which six females had hyperthyroidism secondary to chronic overstimulation of the thyroid by pituitary tsh. in the untreated state, their basal levels of t4 ranged between 14-22 microgram/dl, t3 levels ranged from 205-300 ng/dl, t3 resin uptake ranged from 43-61%, tsh ranged from 5-26 microu/ml, and prl ranged from 33-75 ng/ml. basal metabolic rate (bmr) was elevated in all patients (+32 to +100%). there was no evidence of pituitary tumor, in spite o ...	1982	6798060
pituitary-thyroid feedback regulation in patients with graves' disease during antithyroid drug therapy.	in an attempt to study the mode of normalization of thyroid function in patients with graves' disease, a study was made on 140 patients with graves' disease who were eumetabolic after appropriate therapy with antithyroid drugs for more than 9 months. t3 administration failed to suppress thyroidal radioiodine uptake and serum t4 in patients with trh-unresponsive tsh secretion. in addition, exogenous tsh failed to elevate serum levels of t4 and t3. in patients with trh-responsive pituitaries, t3 a ...	1982	6798061
pattern of recovery of thyroid hormone indices associated with treatment of diabetes mellitus.	the effects of diabetes mellitus on serum thyroid hormone parameters were studied in 54 patients divided into 4 groups. treated asymptomatic patients (group i) had normal thyroid values which did not correlate with serum or urinary glucose. untreated nonketoacidotic patients (group ii) showed a mild persistent depression in t3 and an elevation in rt3. patients with ketoacidosis with a brief history of symptoms (group iii) had a moderate t3 decrease and a mild rt3 elevation which quickly resolved ...	1982	6798065
effects of neonatal hyperthyroidism on the development of the hypothalamic-pituitary-thyroid axis in the rat.	the acute and latent effects of neonatal hyperthyroidism (nh) on the hypothalamic-pituitary-thyroid axis were studied in the rat after treatment of newborn animals with l-t4 (0.4 microgram/g bw, daily) for a period of 12 days. nh was associated with a permanent reduction in body weight in both male and female rats, in addition to a delay in the attainment of peak concentrations of hypothalamic trh and pituitary and serum tsh. serum tsh, t4, and t3 concentrations also were significantly and perma ...	1982	6799277
prevalence of abnormal thyroid function test results in patients with acute medical illnesses.	we measured serum total and free thyroxine (t4) and triiodothyronine (t3) concentrations, free t4 and t3 indexes, thyroid-stimulating hormone (tsh), thyroxine-binding globulin (tbg) and thyroxine-binding prealbumin (tbpa) concentrations in 98 patients hospitalized for acute medical illnesses. the free thyroxine index (ft4i) or tsh level was abnormal in 16 percent, but only 3 percent had thyroid disease. serum fre t4 measurements by equilibrium dialysis were abnormal in 25 percent, but no additio ...	1982	6800256
familial hyperthyroxinemia due to abnormal thyroid hormone binding.	a patient had an elevated serum total thyroxine (t4) free thyroxine (free t4), free t4 index, and t4/thyroxine-binding globulin ratio. the serum triiodothyronine (t3), as well as the thyrotrophin response to thyrotrophin-releasing hormone was normal and consistent with the clinical euthyroid status. the basis for the elevated serum total t4, free t4 index, and t4/thyroxine binding globulin is the abnormally enhanced binding of thyroid hormone by albumin, or by an anomalous protein migrating with ...	1982	6800289
properties of a surface antigen expressed on activated human thymus-derived lymphocytes.	we have identified previously a quantitatively minor membrane protein (p28) with an apparent reduced m.w. of 28,000, which is biosynthetically labeled in activated human lymphocytes. rabbit antisera with activity directed against p28 (alpha-atc) were prepared and p28 was identified by immunoprecipitation in np-40 extracts of activated, extrinsically labeled lymphocytes. p28 was not expressed in appreciable amounts by unstimulated t cells, stimulated or unstimulated b cells, null cells, or adhere ...	1982	6801123
hypothalamic secretion of thyrotrophin releasing hormone is decreased in male japanese quail exposed to long daily photoperiods.	adult male japanese quail held under short daily photoperiods (8 h light: 16 h darkness; 8l: 16d) had significantly higher plasma concentrations of thyroid-stimulating hormone (tsh), tri-iodothyronine (t3) and thyroxine (t4) than did those kept under long days (16l:8d). when given a single s.c. injection of 50 microgram thyrotrophin releasing hormone (trh) the birds held under both the 8l: 16d and 16l: 8d photoperiods showed rapid increases in their blood concentrations of tsh, t4 and t3, the am ...	1982	6801171
autonomous thyroid nodules in adolescents: clinical characteristics and results of trh testing.	seven adolescents with autonomous thyroid nodules were evaluated over a three-year period. they had hyperfunctioning nodules on radionuclide scan which failed to suppress with exogenous administration of thyroid hormone. they were clinically euthyroid and had normal t4, free t4, and basal tsh values. however, as a group they had elevated total serum t3 concentrations, blunted tsh response to trh, and accelerated closure of cranial sutures, all of which suggested subtle hyperthyroidism. these pat ...	1982	6801244
[use of lithium carbonate in the therapy of diffuse toxic goiter].	altogether 22 patients with diffuse toxic goiter and 14 with neurocirculatory dystonia, treated with lithium carbonate, were examined. radioimmunoassay of the blood serum triiodothyronine (t3), thyroxine (t4), and thyrotropic hormone (tth) was used to evaluate the clinical and functional effect of the drug. lithium carbonate in a dose of 900-1800 mg daily during 4-6 weeks produced a significant reduction in t3 level; the level of t4 was less reduced, and the tth concentration increased. the puls ...	1982	6801641
thyroid function tests in patients with acute and resolved hepatitis b virus infection.	serum thyroid hormones and thyroid hormone binding were sequentially measured in 20 patients with acute hepatitis b infection. criteria to select patients consisted of a positive test for hepatitis b surface antigen, aspartate aminotransferase (asat) concentration greater than 400 u/l during the acute illness, and available serum specimens after recovery. the mean serum thyroxine (t4) concentration (+/- se) was 12.5 +/- 0.6 microgram/dl during acute infection and 7.4 +/- 0.3 microgram/dl after r ...	1982	6802052
assessment of optimal l-thyroxine replacement dose by the trh test.	two hundred patients taking varying l-thyroxine replacement doses were studied using a normal trh test as the index of optimal replacement dose. the mean optimal dose was 141 microgram/day. normal serum t3 and ft3i were found in most patients, whatever the trh response, and they are probably too unspecific. serum t4 and ft4i were elevated in many patients with a normal trh response. a higher range for ft4i of 102-166, although only 66.5% accurate, gave the best index of optimal l-thyroxine repla ...	1982	6802063
the nature of human erythrocyte receptors for neisseria gonorrhoeae.	normal and trypsinized human erythrocyte membranes were used as a model in the study of host cell receptors for neisseria gonorrhoeae. receptor sites were identified by adherence inhibition assays of fractions of membranes eluted from polyacrylamide gel electrophoresis columns. results indicated that inhibition of gonococcus t1 and t4 adherence was associated with erythrocyte protein band 3 and 4 and glycophorin a, the major sialoglycoprotein. further investigation revealed that band 3 preparati ...	1982	6802475
radioimmunoassay of "free thyroxin" in dried blood spots on filter paper - preliminary observations on the effective differentiation of subjects with congenital hypothyroidism from those with subnormal thyroxin-binding globulin and normal subjects.	in this sensitive, simple method for measuring "free thyroxin" (ft4) in eluates of dried blood spots on filter paper by use of a radioimmunoassay kit (amerlex free t4 ria), the measurable range of ft4 is 1.8 to 57 ng/l (equivalent to the concentration in serum), or 7 to 237 fg/tube. the mean coefficients of variation for within assay-within spots, within assay-between spots, and between assays were 5.3%, 5.0%, and 6.2%, respectively. ft4 in blood spotted on filter paper is stable for at least a ...	1982	6802516
clomiphene citrate does not modify the exaggerated thyrotrophin response to thyrotrophin-releasing hormone occurring in primary testicular failure.	patients with primary testicular failure have increased basal tsh levels and an exaggerated tsh response to trh in the presence of normal circulating levels of thyroid hormones. in order to evaluate it this tsh profile is an oestrogen-related phenomenon, sixteen patients with primary testicular failure were challenged with 200 micrograms trh prior to and after the administration of clomiphene citrate. the latter was given in a dose of 100 mg/day for 4 weeks to ten patients; 200 mg/day for 4 week ...	1982	6802529
[t4, t3, t3 resin uptake, tbg and free t4 levels for thyroid functions in normal and molar pregnancy (author's transl)].	the production of tbg in liver increases during pregnancy. the thyroid stimulating substance is also suggested to exist in molar pregnancy. we have studied the thyroid functions with t4, t3, t3 resin uptake, tbg, free t4, tsh, hcg, tbg binding capacity and t4 binding by tbg in non-pregnant women, pregnant women and molar pregnant women. t4 and t3 levels were high during pregnancy and remarkably elevated in mole. tbg levels were 49.0 +/- 5.1/micrograms/ml in 3rd. trimester, which were 2.6 times h ...	1982	6802912
are iodine-deficient rats euthyroid?	inhabitants of many severe endemic goiter areas have low serum t4 and high circulating tsh, despite normal levels of t3. this situation may be produced experimentally chronically feeding rats a low iodine diet (lid). we fed rats a remington-type lid and gave them 1% naclo4 in their drinking water for 2 days. after this, the animals were divided into three groups. one group was fed lid, supplemented with 5 micrograms i/rat.day and was used as the control group. another group was fed lid alone. th ...	1982	6804212
synergistic regulation of fetal rat liver nicotinamide adenine dinucleotide phosphate (reduced form) cytochrome c reductase activity: effects of l-triiodothyronine and hydrocortisone.	the activity of hepatic nadph cytochrome c reductase, an enzyme important in drug and steroid metabolism, increases rapidly during the perinatal period in rats. however, the regulation of this increase is not well understood. to investigate the role of hormones in the development of nadph cytochrome c reductase activity, fetal rat livers in organ culture were used in the present study. explants from 20-day-old fetal rat liver could be maintained for up to 96 h in a serum-free medium with or with ...	1982	6804220
seasonal variation of serum thyrotropin concentration and thyrotropin response to thyrotropin-releasing hormone in patients with primary hypothyroidism on constant replacement dosage of thyroxine.	ten patients with primary hypothyroidism (aged 32--66 yr), replaced on constant daily doses of l-t4 (mean +/- sd, 1.90 +/- 0.22 micrograms/kg bw), were used to examine seasonal variations in serum levels of thyroid-related hormones for a period of 14 months. basal and peak tsh concentrations after trh (500 micrograms) were higher in winter than in summer. summer values for basal tsh were all normal (normal range, less than 4.8 microu/ml), while winter values were supranormal in 5 of 10 patients. ...	1982	6804476
sp01 gene 27 is required for viral late transcription.	the sp01 mutant sus ha20 (gene 27) was found to be defective for synthesis of viral late rna. it is known that gene 27 is also required for viral dna replication. the sp01 gene 27 product resembles the t4 gene 45 product, which also has a dual role in viral dna replication and late transcription.	1982	6804643
differing thyrotropin responses to increased serum triiodothyronine concentrations produced by overfeeding and by triiodothyronine administration.	the effects of overfeeding and triiodothyronine (t3) administration on basal serum thyrotropin (trh) concentrations and the tsh response to thyrotropin-releasing hormone (trh) was studied in normal subjects. eight normal volunteers were fed their usual diet plus 2,000 kcal carbohydrate daily for 7 days. their mean serum t3 concentrations increased from 102 +/- 6 (sem) ng/dl to 126 +/- 10 ng/dl; there were no changes in serum thyroxine (t4) and basal serum tsh concentrations or the tsh response t ...	1982	6804747
familial insensitivity of the pituitary and periphery to thyroid hormone: a case report in two generations and a review of the literature.	a clinically euthyroid 2-yr-old girl was found to have diffuse goiter that measured 3 x 5.5 cm with a prominent systolic bruit. serum free t4 (3.4 ng/dl) and serum t3 (360 ng/dl) remained elevated for the next 10 months even though she remained clinically euthyroid. elevation of serum free t4 (3.0 ng/dl) and serum t3 (265 ng/dl) was also present in the 24-yr-old nongoitrous mother who had symptoms and signs of hypothyroidism. following intravenous injection of trh, basal tsh levels of 2.7 and 2. ...	1982	6804753
the effect of thyroid hormones on prolactin secretion by cultured bovine pituitary cells.	the effect of thyroid hormones and thyrotropin releasing hormone (trh) on prolactin (prl) secretion has been studied using a primary calf anterior pituitary cell culture system. after mechanical and enzymatic dispersion, cultured pituitary cells were preincubated with t3 or t4 for 48 hr prior to a 24 hr experimental incubation. t3 stimulated the release of prl into the medium in a dose-related fashion, with an ed50 of 3 nm; at 10 nm t3, a maximal 52 +/- 5% stimulation (p less than 0.001) was obs ...	1982	6804754
growth, development, and reassessment of hypothyroid infants diagnosed by screening.	thirty]six neonates in whom hypothyroidism was diagnosed after thyroid stimulating hormone screening were reassessed at 1 year. all had grown satisfactorily and the mental development scores were normal in all except two. treatment was withdrawn in 32 and persistent hypothyroidism was confirmed in 31 cases. thyroid stimulating hormone concentrations were raised in one-third of cases before the withdrawal of treatment and this was associated with generally lower concentrations of serum thyroxine ...	1982	6805554
thyroxine turnover and transport in viral hepatitis.	thyroxine turnover and transport studies were done on 20 patients in the acute phase of viral hepatitis. no significant changes in t4 degradation rate (k), the t4 distribution volume (v), and the metabolic clearance rate (mcr) were found. a significant increase of t4 serum concentration and tbg capacity, and decrease of tbpa capacity were observed. a negative correlation between tbg and tbpa capacities was found. significant interrelationships between tbg capacity and t4 serum levels as well as ...	1982	6806081
reduction of total and free triiodothyronine in serum after abortion.	the effect of abortion on serum concentration of thyroid hormones was studied in 11 normal women. after abortion, serum free and total t3 fall significantly, while free and total reverse t3, t4, tsh and tbg in serum show no significant variation. these data suggest that surgical stress in normal subjects may only influence 5'-deiodinase activity, and that the peripheral conversion of t4 to active t3 and inactive rt3 are controlled by different mechanisms.	1982	6806082
studies on the hypothalamic control of thyroid function in the rabbit fetuses.	in order to reveal the onset in ontogenesis of hypothalamic control of thyroid function 22-23-day-old fetuses of rabbits were encephalectomized. the concentrations of t3 and t4 in thyroid glands and blood serum were measured radioimmunologically on the 29th and 30th day of prenatal life. significant differences of t3 and t4 concentrations are revealed only in thyroid glands of 30-day-old encephalectomized fetuses. treatment of encephalectomized fetuses by trh 30 min prior to fixation removed the ...	1982	6806084
rna polymerase of myxococcus xanthus: purification and selective transcription in vitro with bacteriophage templates.	dna-dependent rna polymerase from vegetative cells of the gram-negative, fruiting bacterium myxococcus xanthus was purified more than 300-fold by a modified burgess procedure (lowe et al., biochemistry 18:1344-1352, 1979), using polymin p precipitation, 40 to 65% saturated ammonium sulfate fractional precipitation, double-stranded dna cellulose chromatography, a5m gel filtration chromatography, and single-stranded dna agarose chromatography. the last step separated the rna polymerase into a core ...	1982	6806251
familial euthyroid thyroxine excess: characterization of abnormal intermediate affinity thyroxine binding to albumin.	the abnormal high capacity t4 binding site of familial euthyroid t4 excess was separable from prealbumin and t4-binding globulin but not from albumin. we therefore compared t4 binding by albumin preparations isolated from the sera of normal and affected subjects. by equilibrium dialysis, albumin from affected subjects showed an extra t4 binding site (kd approximately 50 nm) in addition to the t4 binding sites of normal albumin (kd approximately 4 microm). comparison of the estimated capacity of ...	1982	6806311
new regulators of melanogenesis are associated with purified tyrosinase isozymes.	three new regulatory factors in the melanogenesis pathway were recently described: dopachrome conversion factor accelerates the conversion of dopachrome to 5,6-dihydroxyindole; indole conversion factor accelerates the conversion of 5, 6-dihydroxyindole into melanin; and indole blocking factor retards the conversion of 5, 6-dihydroxyindole into melanin. exposure of wild-type cloudman melanoma cells in culture to melanotropin (msh) removes blocking factor activity and increases indole conversion f ...	1982	6806397
[studies on streptococcal t antibodies. ii. clinical and epidemiological studies on streptococcal type t4-specific antibody examined by hemagglutination reaction (author's transl)].		1982	6806411
discrimination of protein and nucleic acids by electron microscopy using contrast variation.	a contrast variation method is described by which protein and nucleic acid components of nucleoprotein assemblies can be distinguished in the electron microscope. using contrasting media of variable electron scattering density, the contribution to image contrast of protein or nucleic acid can be matched out. the protein and dna components of t4 bacteriophages were visualized separately in this way. the distribution of rna and protein in ribosomes was studied by processing images of crystalline s ...	1982	6803416
elevated serum thyroxine-binding globulin by x-chromosome transmission.	a kindred with a high prevalence of hereditary serum thyroxine-binding globulin (tbg) is described. seventeen of th 29 members of the kindred have the increased tbg trait as demonstrated by a combination of increased serum total thyroxine (t4) and total triiodothyronine (t3) and decreased t3 resin uptake. in 12 of the 17, the anomaly was confirmed by measuring serum tbg activity as maximum binding capacity of t4. the pattern of increased tbg is consistent with x-linked inheritance. one of the af ...	1982	6803486
thyrotrophin-releasing hormone responsiveness and degradation in children with chronic renal failure: effect of time of evolution.	in order to study the hypothalamic-pituitary-thyroid function in children with chronic renal failure (crf), the serum levels of l-thyroxine (l-t4), l-triiodothyronine (l-t3), reverse t3 (rt3), thyrotrophin (tsh) and prolactin (prl) were measured by radioimmunoassay (ria). values were compared with those of normal subjects. low levels of l-t4 were present in crf patients as compared to controls. l-t3 was also found to be low but less than l-t4, and rt3 was lower in patients with long evolution. n ...	1982	6803491
black thyroid. morphological, biochemical and geriatric studies on the brown granules in the thyroid follicular cells.	an autopsy case of a 73-year-old man with "black thyroid" was reported. investigations by light and electron microscopy and histochemical study of the black thyroid material disclosed the massive deposition brown granules in the follicular cells, to be residual bodies containing lipids. lipids in these granules were mainly composed of phospholipid by biochemical analysis. concerning the thyroidal function of this case the value of t4-i in the serum was within normal range (4.8-7.5 micrograms/dl) ...	1982	6803514
the t4:tbg ratio: a re-evaluation with particular reference to low and high serum tbg levels.	the thyroxine: thyroxine-binding globulin (t4: tbg) ratio is now an established part of the biochemical investigation of thyroid function. reference ranges have been reported for euthyroid subjects with tbg levels within the range 6-16 mg/l. routine assay of tbg on all thyroid function tests in this laboratory has suggested that, in patients with low or high tbg levels, the established reference ranges for t4: tbg may not be strictly applicable. a retrospective study has been made of a large num ...	1982	6803653
bromocriptine therapy in oligozoospermic infertile men.	a prospective, randomized double-blind study with crossover using bromocriptine and placebo was performed on a group of 17 infertile males with idiopathic oligozoospermia. twelve patients completed the duration of this study of eight months by receiving 5 mg of bromocriptine per day for four months followed by four months of placebo or vice versa. prior to treatment, the sperm count was 8.76 +/- 1.32 (10(6)/ml). the hormonal profile was performed prior to treatment and included estimation of pro ...	1982	6803692
a comparison of t4 and t7 dermatomal levels of analgesia for caesarean section using the lumbar epidural technique.	we compared analgesia to the t4 dermatomal level with analgesia to the t7 level with and without prophylactic intramuscular administration of ephedrine 25 mg to determine the adequacy and side effects of such analgesia for caesarean section. unmedicated patients were prehydrated (727 +/- 303 ml of saline solution) and kept in a left lateral tilt position. sufficient three per cent chloroprocaine was given to obtain analgesia to the t7 (t6-t8) dermatomal level (455 +/- 128 mg) or to the t4 (t3-t5 ...	1982	6804068
immunoglobulin binding of thyroid hormones in a case of waldenstrom's macroglobulinemia.	this paper describes a case of hypothyroidism in a patient with waldenström's disease in which the evidence of thyroid failure was accompanied by an abnormal binding of thyroid hormones in the gamma-globulin fraction. a 68-yr-old patient with waldenström's disease appeared to be hypothyroid by clinical and laboratory criteria. serum tsh was elevated; serum t3 (measured by ria) was low, while t4 levels were undetectable or very high according to the method used. serum free thyroid hormones were i ...	1982	6801074
comparison of pituitary-thyroid function in patients with endstage renal disease and in age- and sex-matched controls.	the response to i.v. bolus thyrotropin-releasing hormone (trh) of 14 dialysis patients with end-stage renal disease (esrd) was compared to the response of 14 age- and sex-matched renal clinic patients (controls) with normal renal function (serum creatinine concentrations less than 1.2 mg/dl). the mean basal serum levels of thyrotropin (tsh) were similar in the two groups. there was no difference between the two groups in the mean maximal increase in tsh after trh (6.3 microu/ml and 7.2 microu/ml ...	1982	6803061
serum t4, t3 and tbg concentrations during puberty in males.	measurements of serum thyroxine (t4), thyroxine binding globulin (tbg), and triiodothyronine (t3), using ria, were conducted in healthy israeli boys, 149 children from the age 8 years were divided into five groups according to tanner's puberty stages. the correlation with tanner's staging showed a significant decrease in t4 and tbg concentrations during puberty. however, t3 decreased only toward the last stage of puberty (between p4 to p5). our data indicate that during puberty in male subjects ...	1982	6807675
prolactin response to thyrotropin-releasing hormone in children with turner's syndrome and hyperthyroidism.	plasma prolactin (prl) response to synthetic thyrotropin-releasing hormone (trh) was studied in 26 prepubertal and 19 pubertal children with constitutional short stature, 7 patients with turner's syndrome and 10 patients with hyperthyroidism. the mean basal concentrations of plasma prl did not differ among groups. in prepubertal children prl responses to trh were comparable in both sexes, while pubertal children plasma prl levels after trh in females were significantly higher (p less than 0.05) ...	1982	6807677
thyroid function and chronic hepatosplenic schistosomiasis: peripheral conversion of thyroxine to 3,5,3'-triiodothyronine and pituitary-thyroid relationship.	the relationship between chronic hepatosplenic schistosomiaisis (ches) and circulating thyroid hormones as well as the tsh response to trh were investigated in 41 hospitalized ches patients and compared to those in 11 patients with non-ches cirrhosis with severe hepatic failure. ches patients were subdivided into 3 groups depending on the severity of parenchymal dysfunction, based upon a composite clinical and laboratory index. angiographic and hemodynamic studies of ches patients revealed alter ...	1982	6808003
human lymphocytes with either the okt4 or okt8 phenotype produce interleukin 2 in culture.	in this study, we demonstrate that both highly purified t4+ and t8+ lymphocytes can produce substantial amounts of interleukin 2(il 2) when stimulated with the combination of concanavalin a (con a) and phorbol myristate acetate. furthermore, addition of il 1 to macrophage-depleted t lymphocytes significantly increased il 2 production by lymphocytes of either the t4+ or t8+ phenotype. these findings provide a basis for further studies of the molecular mechanisms involved in human immune cell inte ...	1982	6808028
[the metabolism of thyroid hormones in chronic renal insufficiency].	the levels of tri-iodothyronine (t3), free t3 index (ft3i), thyroxine (t4), free t4 index (ft4i), thyrothopine (tsh), reverse t3(rt3), t3 resin uptake (t3u) and thyroxine-binding globulin (tbg) were investigated in 30 conservatively treated euthyrodic kidney patients with varying degrees of disturbance to kidney function. the authors found that the phenomena of the so-called "low t3 syndrome" (low t3 and ft3 while t4 is low to normal and ft4 and tsh are normal) appear not only in patients with a ...	1982	6808788
tests of thyroid function important to the ophthalmologist.	the most useful tests to establish a diagnosis of endocrine exophthalmos are the following: to prove that thyroid gland activity is increased: serum t3 and t4 assays. to demonstrate immunologic abnormalities: thyroid antibody testing and the tda test. to demonstrate an abnormal hypothalamic-pituitary-thyroid axis: the trh test. to demonstrate enlarged ocular muscles: orbital ultrasonography and computerized tomography.	1982	6809298
propranolol improves the impaired tsh response to trh in patients with autonomously functioning euthyroid multinodular goitre.	ten patients with autonomously functioning euthyroid multinodular goitre received propranolol (inderal retard 160 mg daily) for 4 weeks in order to investigate whether normalization of the trh test or an increased tsh response to trh could be obtained by decreasing serum t3 levels. serum t3 decreased significantly after 2 and 4 weeks of propranolol administration. serum t4 increased during this period, although the change was only significant after 4 weeks of propranolol. t3 resin uptake did not ...	1982	6809365
effect of diabetic control on the level of circulating thyroid hormones.	basal plasma levels of thyroxine (t4), triiodothyronine (t3) and reverse t3 were determined by radioimmunoassay in 44 control subjects, 44 type 1 (insulin-dependent) and 39 type 2 (non insulin-dependent) diabetic patients aged from 15 to 75 years. all were clinically euthyroid. the quality of diabetic control was assessed by the percentage of glycosylated haemoglobin. in both the diabetic groups there was a significant decrease in t3 and a rise in reverse t3 whereas t4 was normal. we found no si ...	1982	6809514
reduction of the fraction of circulating helper-inducer t cells identified by monoclonal antibodies in psoriatic patients treated with long-term psoralen/ultraviolet-a radiation (puva).	ultraviolet radiation has been found to alter the distribution and function of human lymphocytes. to determine whether photochemotherapy (puva) alters circulating levels of t cell subset marker-bearing lymphocytes, cells from 9 patients with psoriasis undergoing puva therapy for several years (mean 4.6 +/- 1.4 yr), 17 patients with active untreated psoriasis, and 20 healthy volunteers were reacted with monoclonal antibodies to t cell surface markers, including okt3 (all peripheral blood t cells) ...	1982	6809841
"symptomless" autoimmune thyroiditis in depression.	the magnitude of the thyroid-stimulating hormone (tsh) response induced by thyrotropin-releasing hormone (trh) helps identify patients whose thyroid is failing. many of these patients have been found to have hashimoto's thyroiditis, symptomless autoimmune thyroiditis (sat), and subclinical hypothyroidism. while patients with sat are clinically euthyroid, what might be "symptomless" for the endocrinologist might be a syndrome presenting with psychiatric symptoms to the psychiatrist. as a prelimin ...	1982	6810401
advanced carcinoma of the nasopharynx. a clinical study of 274 patients.	a total of 274 patients with a diagnosis of nasopharyngeal carcinoma was treated in eight veterans administration hospitals over a period of 22 years. of the 274 patients, 256 (93%) had squamous-cell carcinoma, while 18 (7%) had other tumors. most of the squamous-cell carcinoma patients (82%) had stage iv disease; cervical lymph node metastases were found in 193 (75%), and distant metastases were present in 22 (9%). the actuarial 5-, 10-, and 15-year survival rates for the 256 squamous-cell carc ...	1982	6810406
effects of dexamethasone on the hypothalamic-pituitary-thyroid axis in rats.	effects of dexamethasone on the hypothalamic-pituitary-thyroid axis in rats were studied. rats given saline (group a), 25 micrograms of dexamethasone/100 g body weight (group b) or 500 micrograms o dexamethasone/100 g body weight (group c) were serially decapitated and brain tissues and blood were obtained. trh contents in the hypothalamus, plasma concentrations of trh, tsh, t4, t3 and reverse t3 were measured by specific radioimmunoassay. trh contents in the hypothalamus were significantly incr ...	1982	6810611
the oral trh stimulation test. a new diagnostic tool in "non-toxic" multinodular goitre.	we have developed a 28-hour trh stimulation test based on the ft3i response to repeated oral trh stimulation. the normal ft3i response in 15 controls was greater than or equal to 40 arb.u. twenty-five euthyroid patients with multinodular goitre were studied. fourteen had normal ft3i responses and normal basal levels of serum t4, ft4i, serum t3 and ft3i. eleven patients had ft3i responses below 20 arb.u. although the patients' individual basal values of serum t4, ft4i, serum t3 and ft3i were wit ...	1982	6810657
partial hypopituitarism and hyperprolactinemia: successful induction of ovulation with bromocriptine and human menopausal gonadotropins.	the case of a patient who developed partial hypopituitarism (hypogonadotropism and growth hormone deficiency) following transphenoidal removal of a prolactinoma is described. hypogonadotropism persisted despite restoration of normoprolactinemia with bromocriptine therapy. successful induction of ovulation with human menopausal gonadotropin (hmg) and bromocriptine suppression of the hyperprolactinemia was carried out, resulting in a pregnancy. the pros and cons of operative and nonoperative manag ...	1982	6811337
effect of prednisone on serum and urinary thyroid hormone levels in children during the nephrotic syndrome.	thyroxine (t4), triiodothyronine (t3), free t4, and thyroxine binding globulin (tbg) were measured in serum and urine, thyrotropin (tsh) only in serum, in children with nephrotic syndrome (ns) of less than one month's duration, in children with the disease for more than one month and with steroid treatment, and in children in total or partial remission. there was a significant decrease in serum t4, t3 and tbg in nephrotic children compared to normal children. there was no change in free t4 but a ...	1982	6811505
periodicity in the length of 3'-poly(a) tails from native globin mrna of rabbit.	globin mrna from rabbit reticulocytes was labelled at the 3'-end with [5'-32p]pcp by t4 rna ligase. the 3'-poly(a) tail was released by digestion of mrna with t1 ribonuclease and its size distribution determined by gel electrophoresis and autoradiography. the length of the 3'-poly(a) tails varied from about 15-150 residues, but the size distribution exhibited peaks in the abundance of poly(a) species at intervals of approx. 25 residues. this periodicity appears to reflect the manner in which pro ...	1982	6812023
thyroid associated components in serum during normal pregnancy.	thyroxine-binding globulin (tbg(, thyroxine-binding pre-albumin (tbpa), thyroxine (t4), free-t4, triiodothyronine (t3), and thyroid-stimulating hormone (tsh) were studied throughout apparently normal pregnancy in 290 cases grouped in 2 week intervals. tbg increased to a plateau level reached in the 24th week and was hereafter unchanged until term. t4 showed an increase until the 16th week of pregnancy and levelled off to a constant level for the rest of the pregnancy. free-t4 declined to almost ...	1982	6812340
abnormalities in thyroid function tests in patients admitted to medical service.	serum thyroid hormone, thyrotropin (tsh) and thyroxine-binding globulin (tbg) concentrations, free thyroxine index values, and free thyroxine concentrations were measured at the time of admission in all 77 patients hospitalized on a medical service on four separate days. serum thyroxine (t4) concentrations and serum free t4 index values were decreased in 19.5% and 11.7%, respectively, and increased in 3.9% and 11.7%, respectively; serum free t4 concentrations were decreased in 6.8% and increased ...	1982	6812519
developmental changes in pituitary-thyroid function in the human fetus and newborn.	maturation of the hypothalamic pituitary thyroid axis as reflected in cord serum thyroid hormone concentrations was assessed in premature and full term infants born between 26 and 43 weeks gestation. measurements of thyroxine (t4), free t4 (ft4), thyrotropin (tsh) and thyroxine binding globulin (tbg) in cord sera were correlated with gestational age, sex and birthweight and compared to similar measurements in well two month old infants and adults. there were significant increases in t4, ft4, and ...	1982	6813103
thyroid function in children with cystic fibrosis.	serum concentrations of t4, t3, reverse t3 (rt3), tsh, thyroxine binding globulin (tbg) and thyroxine binding prealbumin (tbpa) were measured and a trh-stimulation test was performed in 10 iodide untreated children affected by cystic fibrosis (cf) and in 84 controls. as compared to the controls, cf patients had lower t4 and rt3, similar t3 and tbg and increased t3:t4 ratios. they also had lower tbpa, but this could not account for the low t4. finally they had higher basal and trh-stimulated tsh. ...	1982	6813123
are selective increases in serum thyroxine (t4) due to iodinated inhibitors of t4 monodeiodination indicative of hyperthyroidism?		1982	6813344
function of pituitary-thyroid axis in copper-deficient rats.	thirty male weanling sprague-dawley rats were fed either a copper deficient diet (0.85 mg cu/kg) or a copper-adequate diet (8 mg cu/kg). after 7 weeks, the rats were fasted for 12 hours, and injected intravenously with thyrotropin-releasing hormone (trh, 30 ng/100 g body weight). six rats from each treatment were killed at 0, 0.5, 1, 2 and 4 hours after trh injection. sera obtained at 0, 0.5 and 1 hour, and at 0.2 and 4 hours were used for the radioimmunoassay of thyroid-stimulating hormone (tsh ...	1982	6813435
[comparison of free thyroxine index, t4/tgb ratio, and t4/unbound tbg as an indirect indicator of blood free thyroxine].		1982	6813544
metabolic responses of the rooster after exogenous thyroid hormones.	total sensible heat loss, o2 consumption, and co2 production were monitored in 20 white leghorn roosters (1.85 +/- .05 kg body weight) subjected to either saline, or triiodothyronine (20 micrograms/kg; t3), or thyroxine (50 micrograms/kg; t4) administered in a single dose. treatment occurred after a 26-hr fast and the fast was maintained for the duration of the experiment. plasma t3 concentrations in birds treated with t3 were higher than controls for an 8-hr period after treatment (p less than ...	1982	6813837
[neonatal electrocardiographic manifestations of congenital hypothyroidism. correlation with echocardiographic data].	twelve infants, average age 5, 4 weeks (range 3 to 8 weeks) with congenital hypothyroidism were studied. in addition to routine evaluation including plasma t3, t4 and tsh estimation and the establishment of a clinical index of hypothyroidism on electrocardiogram and an echocardiogram were performed. the following variables were analysed: heart rate, qrs axis in the frontal plane, pr interval in lead ii, corrected qt interval in v5, amplitude of the p waves in lead ii, r waves in v3r, v1, v5 and ...	1982	6814384
are pyrimidine dimers tolerated during dna replication of uv-irradiated parvovirus minute-virus-of-mice in mouse fibroblasts?	the replication of the single-stranded dna of parvovirus minute-virus-of-mice (mvm) was depressed when virus was exposed to uv-light prior to infection of mouse fibroblasts. most of the viral dna containing pyrimidine dimers was permanently blocked in its conversion to double-stranded replicative forms (rf). yet dimers might be tolerated to a low extent, considering that a minor fraction of parental rf molecules was sensitive to the action of the uv-specific endonuclease v of bacteriophage t4, u ...	1982	6814534
a bacteriophage t4 dna packaging related dna-dependent atpase-endonuclease.	a new bacteriophage t4-induced dna-dependent atpase-endonuclease was purified to essential homogeneity from an extract of late infected escherichia coli. both dna-dependent atpase and endonuclease activities co-chromatograph, co-sediment, and have been renatured from a single 43-kilodalton protein eluted following sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting that both activities are exerted by one multifunctional protein. duplex, single-stranded, and supercoiled dnas are ...	1982	6815174
pituitary-thyroid axis after bilateral orchiectomy in men.	the functional interaction between the testis and thyroid was evaluated in 13 patients affected by prostatic carcinoma before and after bilateral orchiectomy. two different patterns of thyroid hormones were noted. immediately after surgery, free thyroxine (t4) increased and total triiodothyronine (t3) decreased probably as a result of surgical stress at anesthesia. later, free t4 levels decreased while total t4 and thyroxine binding globulin (tbg) were unchanged. such changes may be due to quali ...	1982	6816159
structure-function relationships in escherichia coli translational elongation factor g: modification of lysine residues by the site-specific reagent pyridoxal phosphate.	translational elongation factor g (ef-g) of escherichia coli was modified with the selective, site-specific lysine reagent pyridoxal phosphate (plp). the reaction results in the modification of a maximum of 12 lysine residues, one of which is essential for guanosine 5'-triphosphate (gtp) binding and whose modification is inhibited by the presence of gtp. formation of a reversible adduct between 2,3-butanedione and an essential arginine similarly located in the gtp binding site [rohrbach, m.s., & ...	1982	6816267
thyroid abnormalities in patients with myotonic dystrophy.	myotonic dystrophy (md) is a systemic genetic disorder predominantly affecting musculature. although endocrine disturbances have been described, data relating to thyroid gland involvement are sparse and conflicting. to further investigate this aspect, twenty md patients and twenty age-and sex-matched controls were studied. all were clinically euthyroid and there were no significant differences between circulating levels (mean +/- sd) of t4 (101 . 5 +/- 28.2 v. 103 . 8 +/- 27 . 7 nmol/l), t3 (1 . ...	1982	6816488