Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| immunomodulatory role of thyroid hormones: in vivo effect of thyroid hormones on the blastogenic response of lymphoid tissues. | in an attempt to find out the mechanism of immunomodulation by thyroid hormones (t3 and t4), their in vivo effect on the blastogenic response of lymphocytes from various lymphoid tissues of hormone-treated and thyroidectomized rats were studied. the blastogenic response of lymphocytes from thymus, peripheral blood and mesenteric lymph nodes to pokeweed mitogen (pwm) was found to be increased significantly following t3 or t4 administration for 15 days or 30 days. however, the response to phytohae ... | 1983 | 6602446 |
| phenotypic diversity within clones of human normal t cells. | human normal t cells were selected for in vitro cloning according to the expression of t4, t8 or t10 antigens on individual cells. clones were produced from each of these cells irrespective of the antigenic phenotype of the parental cell. the cloned progeny manifested, in many cases, shifts in antigen expression. thus, t4+t8- cells gave clones expressing predominantly t4-t8+ and vice versa. the clonal expression of t4 and t8 seemed to be mutually exclusive. antigenic shifts were recorded also in ... | 1983 | 6602777 |
| evaluation of t lymphocyte subpopulations in children with nephrotic syndrome. | we have evaluated lymphocyte subsets both quantitatively and functionally in children with nephrotic syndrome. ten children had minimal change nephrotic syndrome (mcns), seven had focal segmental glomerular sclerosis (fsgs) and four had recovered from mcns. two patients with fsgs had decreased okt4+ cells and a decreased t4/t8 ratio. patients with active mcns synthesized significantly less igm in vitro than did normal subjects (p less than 0.005). in vitro igg synthesis was significantly decreas ... | 1983 | 6603300 |
| t-cell subpopulations in the peripheral blood of patients with connective tissue diseases as determined by flow cytometry using monoclonal antibodies. | we studied by flow cytometry using monoclonal antibodies the t3, t4, and t8 subpopulations of t cells in the peripheral blood of 109 patients with various connective tissue diseases who were not receiving any treatment. comparison of the results was made with those obtained with normal controls matched for age and sex with each connective tissue disease group. when compared as disease groups, patients with systemic lupus erythematosus (n = 41) had decreased t8 cells, but patients with active dis ... | 1983 | 6603315 |
| a serum-free, chemically-defined medium for function and growth of primary neonatal rat heart cell cultures. | a serum-free, chemically defined medium for supporting rhythmic contraction, maximum survival, and moderate growth of cardiac cells was achieved by using a combination of hormones and growth supplements in a mixture of equal volumes of ham's f12 and dulbecco's modified eagle's medium. the hormones and growth supplements included insulin, transferrin, selenium, fetuin, bovine serum albumin, hydrocortisone (hc), l-thyroxine (t4), and epidermal growth factor (egf). cardiac cells were grown on fibro ... | 1983 | 6603410 |
| phorbol ester modulation of t-cell antigens in the jurkat lymphoblastic leukemia cell line. | the effect of phorbol dibutyrate (pdb) on the cell surface antigens of the human t-cell acute lymphoblastic leukemia cell line, jurkat, was studied with okt monoclonal antibodies by indirect immunofluorescence assay. cells were analyzed in an ortho spectrum iii fluorescence-activated flow cytometer. the surface antigen profile of untreated jurkat cells resembled that of thymocytes; high levels of t3, t4, t6, t8, t9, t10, and t11 antigens were detected. although 89% of cells were positive for t11 ... | 1983 | 6603901 |
| evaluation of thyroxine monodeiodination to reverse triiodothyronine by tissue homogenates in vitro. | reverse-triiodothyronine (rt3) generated from thyroxine (t4) in vitro at physiological ph (7.4) is very labile and rapidly undergoes further enzymic deiodination to diiodothyronine. the paper describes the procedure in which by different ph optima, t4 to rt3 conversion distinct from t4 to t3 monodeiodination can be traced at ph 9.0, in the presence of dithiothreitol (dtt) as a donor of sulfhydryl groups. the net in vitro production of rt3 from exogenous t4 was thus measured utilizing specific ri ... | 1983 | 6603967 |
| the 3'-5' proofreading exonuclease of bacteriophage t4 dna polymerase is stimulated by other t4 dna replication proteins. | the bacteriophage t4 dna polymerase has an intrinsic 3'-5' proofreading exonuclease activity that plays a central role in determining the fidelity of t4 dna replication. in order to monitor this activity, we have measured the rate at which the polymerase decreases the size of a double-stranded dna substrate in the absence of deoxyribonucleoside triphosphates. with this assay, we find that the addition of the polymerase accessory proteins, 45 protein and 44/62 protein, increases the rate at which ... | 1983 | 6604051 |
| antigen-like effects of monoclonal antibodies directed at receptors on human t cell clones. | recent studies suggested that the clonally unique ti epitopes defined by non-cross-reactive monoclonal antibodies might represent the variable regions of the antigen receptor. here we determine whether such anti-ti antibodies could trigger clonal t cell activation. anticlonotypic monoclonal antibodies to the 49/43-kdalton heterodimer of a given clone or antibodies to the 20/25-kdalton membrane associated monomorphic t3 molecule selectively induce proliferation and il-2 secretion when linked to a ... | 1983 | 6604129 |
| phosphorylation of human growth hormone by the epidermal growth factor-stimulated tyrosine kinase. | in the present study, we have demonstrated that human growth hormone (hgh) can be phosphorylated by the epidermal growth factor (egf)-stimulated tyrosine kinase of a431 cell membranes. phosphotyrosine was the predominant phosphoamino acid released from phosphorylated hgh on partial acid hydrolysis. all five tyrosine-containing tryptic peptides of hgh are also phosphorylated by the egf-stimulated tyrosine kinase. the highest phosphate incorporation was found for peptide t4 (residues 20-38), which ... | 1983 | 6604272 |
| serologic and immunopathologic findings in temporal arteritis. | in 104 patients undergoing biopsies for temporal arteritis, lymphocyte characterization identified both t4 helper/inducer and t8 cytotoxic/suppressor lymphocytes in approximately equal numbers. b lymphocytes were absent. deposition of igm and igg was observed in three of 16 positive biopsy specimens. antinuclear antibodies were present in ten of 16 biopsy-proven cases of temporal arteritis compared with 19 of 55 in the control group with negative biopsy specimens. anti-smooth-muscle, anti-dna, a ... | 1983 | 6604457 |
| immunocompetent cells in psoriasis. in situ immunophenotyping by monoclonal antibodies. | immunocompetent cells in exacerbating untreated psoriasis vulgaris skin lesions were immunophenotypically studied by the application of a selection of monoclonal antibodies in a two-stage immunoperoxidase technique. epidermal changes include: focal accumulation of immunoglobulins in the stratum corneum, as demonstrated by a mixture of monoclonal anti-kappa and anti-lambda antibodies; focal accumulation of okm-1 positive but mo-2 negative cells high in the epidermis, reflecting granulocytes in mu ... | 1983 | 6604503 |
| the ultrastructural morphology of t lymphocytes in b-chronic lymphocytic leukaemia: a study with monoclonal antibodies and the immunogold technique. | t-lymphocytes from five patients with b-cell chronic lymphocytic leukaemia (b-cll) were analysed by light (lm) and electron microscopy (em) by means of the immunogold technique with monoclonal antibodies combined with e rosettes. lm analysis confirmed the existence of a population of e+ lymphocytes unreactive with the okt3 monoclonal antibody. the em study showed that e+ lymphocytes from b-cll can be distinguished morphologically from the leukaemic b-cells which were identified by their labellin ... | 1983 | 6604542 |
| cellular bases of the production of and response to interleukin-2 in man: role of autologous rosette-forming t-cell subsets defined with monoclonal antibodies. | in this paper we present experiments that indicate that, in man, most t-cell subpopulations produce interleukin-2 (il-2), but that the main cell subpopulation which produces it, both upon activation with phytohaemagglutinin or in autologous mixed lymphocyte cultures, is that of autologous rosette-forming (tar) t4+ t cells. conversely, the main il-2-responding t-cell subpopulation is that composed of t cells depleted of tar (t-tar) that are t8+. il-2 was also found to be more effectively produced ... | 1983 | 6604692 |
| attachment of tail fibers in bacteriophage t4 assembly. identification of the baseplate protein to which tail fibers attach. | a phage-neutralizing rabbit antiserum collected after immunization with tail-fiberless bacteriophage t4 particles was adsorbed with complete t4 phage. the resulting adsorbed serum inhibited tail fiber attachment in vitro. to identify the antigens against which this inhibitory activity was directed, blocking experiments were carried out with the adsorbed serum. isolated complete baseplates and mutant-infected-cell extracts lacking known baseplate gene products but containing gene 9 product showed ... | 1983 | 6604820 |
| recognition of chemically damaged dna by the gene 32 protein from bacteriophage t4. | we have used fluorescence spectroscopy to investigate the binding of gene 32 protein from bacteriophage t4 to dna which has been chemically modified with carcinogens or antitumor drugs. this protein exhibits a high specificity for single-stranded nucleic acids and binds more efficiently to dna modified either with cis-diaminodichloroplatinum(ii) or with aminofluorene derivatives than to native dna. this increased affinity is related to the formation of locally unpaired regions which are strong b ... | 1983 | 6605246 |
| isolation and characterization of precursors in bacteriophage t4 baseplate assembly. ii. purification of the protein products of genes 10 and 11 and the in vitro formation of the p(10/11) complex. | two bacteriophage t4 proteins which are precursors to the phage baseplate have been purified to homogeneity. these proteins, p10 and p11, are components of the p(10/11) complex, which is the first intermediate in the assembly of t4 baseplate 1/6th arms. each protein was isolated from cells infected with a t4 amber mutant defective in the production of the other protein. thus these purified proteins have never been assembled into the p(10/11) complex in vivo. sodium dodecyl sulfate/polyacrylamide ... | 1983 | 6605425 |
| distribution of t-cell subsets in follicular and diffuse lymphomas of b-cell type. | the authors examined the number and distribution of cells reacting with monoclonal antibodies to t-cell subsets in frozen tissue sections of b-cell lymphomas (30 follicular and 17 diffuse lymphomas). in five diffuse lymphomas (two lymphocytic, three small cleaved cell) the neoplastic b-lymphocytes reacted with the monoclonal antibody anti-t1. in all other cases, the monoclonal antibodies to t-cell subsets reacted only with small lymphocytes concentrated between the follicles of follicular lympho ... | 1983 | 6605689 |
| a longitudinal study of a patient with acquired immunodeficiency syndrome using t cell subset analysis. | acquired immunodeficiency syndrome (aids) has recently been documented in patients in association with opportunistic infections, kaposi's sarcoma, cytomegalovirus infections, and recreational drug use. aids is characterized by cutaneous energy, diminished peripheral lymphocyte responses to mitogens and antigens, and abnormal t cell subpopulations. these patients have been described as having decreased total t (t3+) cell numbers, virtual elimination of the t helper (t4+) cell population, and an i ... | 1983 | 6606305 |
| immunoregulatory abnormalities in evans syndrome. | immune function in six patients with evans syndrome (coombs-positive hemolytic anemia and immune thrombocytopenia) was compared to that in seven with chronic itp. the two groups differed in measurements of t-cell subsets and immunoglobulin production. evans syndrome patients had decreased t4 (t-helper) (p = 0.025), increased t8 (t-suppressor) (p = 0.008), and a decreased ratio of t4:t8 cells (p = 0.0009) when compared to controls. results in chronic itp patients were similar to those in controls ... | 1983 | 6606357 |
| human autologous rosette-forming cells. i. expression of cell surface antigens in relation to age and lymphoid organ distribution. | autologous rosette-forming cells (auto-rfc) were characterized with monoclonal antibodies to various cell surface antigens using a technique combining immunofluorescence and rosette formation. in peripheral blood, auto-rfc were t cells (leu 1+/okt3+) the majority being derived from the helper/inducer subset (leu 3a+/okt4+). a small proportion of the circulating auto-rfc were leu 2a+/okt8+ and virtually none of them bore t10, t6, and dr antigens or peanut agglutinin (pna) receptors. in the elderl ... | 1983 | 6606493 |
| [age- and sex-dependent changes in human t-lymphocyte subpopulations]. | changes in the expression of t-lymphocyte membrane antigens are seen in many diseases, particularly in autoimmune diseases. the reliability of these parameters, as well as their changes associated with age and sex, were examined in 232 healthy subjects using monoclonal antibodies of the okt series. the precision of the indirect immunofluorescence method for determining the t3, t4 and t8 antigens (corresponding antibodies okt 3, okt 4, okt 8) is within a range expected for biological testing meth ... | 1983 | 6606556 |
| passage of thyroid hormone into milk in rabbits. | in lactating rabbits of new-zealand breed the passage of iodothyronines from blood to milk was studied. the iodothyronines from the milk were extracted with different solvent systems and then separated either with the aid of paper chromatography in butanol-ethanol-ammonia system or on a sephadex g-25 fine columns with the use of alkalized ethanol. at 4 h after the administration of labelled compounds the milk/serum ratio for thyroxine (t4) was 0.3, for triiodothyronine (t3) 1.2 to 2.8 and for re ... | 1983 | 6606565 |
| serum thyroid hormone levels in colostrum deprived piglets and calves. | the level of thyroxine (t4), triiodothyronine (t3) and reverse triiodothyronine (rt3) was measured in blood of colostrum deprived calves and piglets and compared with appropriate control animals which were fed colostrum and milk according to a regular farm schedule. in most of cases, the levels of iodothyronines measured were significantly decreased in colostrum deprived (cd) calves between 36 h and 14 d of age. in piglets, the levels of iodothyronines were measured up to 72 h of age only and no ... | 1983 | 6606566 |
| studies of lymphocyte subpopulations in the liver tissue and blood of patients with chronic active hepatitis (cah). | monoclonal antibodies to antigens on the surfaces of mononuclear cells (mnc) were used to characterize lymphocyte subpopulations infiltrating portal areas and parenchyma of livers in 31 patients with chronic active hepatitis (cah). the distribution and numbers of infiltrating lymphocytes were determined in serial sections immunostained by the avidin-biotin-peroxidase complex method. t lymphocytes were the major component of inflammatory cells in the portal tracts. in the peripheral blood and por ... | 1983 | 6606644 |
| surface markers on lymphocytes from human cerebrospinal fluid. identification by monoclonal antibodies. | lymphocyte subpopulations in cerebrospinal fluid (csf) and peripheral blood (pb) were studied using monoclonal antibodies and the common membrane markers. the results in three groups of patients were compared: 36 subjects with 'non-immunological disorders' (nid), 14 subjects with multiple sclerosis (ms) and 6 with subacute sclerosing panencephalitis (sspe). it was found that, in patients with nid, (1) 90% of cells were t lymphocytes, reactive with okt3; (2) the helper/suppressor (t4/t8) ratios w ... | 1983 | 6606648 |
| mononuclear cell subpopulations during normal pregnancy: i. analysis of cell surface markers using conventional techniques and monoclonal antibodies. | previous reports indicate depression of cellular immunity during pregnancy. in order to get a deeper insight into the immunoregulatory subsets of mononuclear cells in pregnant women, we analyzed the percentages of t and b lymphocytes, t cell subsets and cells expressing igg-fc and c3 receptors, and m1- and ia-like antigens in venous blood samples from healthy women and pregnant women at different gestational ages and after delivery. even if a trend toward a progressive decrease of t lymphocytes ... | 1983 | 6606987 |
| thyroid function tests in thyroid and nonthyroid disease. | modern day evaluation of thyroid disorders requires a combination of accurate clinical judgement and reliable, sensitive, and specific thyroid functions tests. principle among the latter are thyroxine (t4) 3, 5, 3'-triiodothyronine (t3), and thyroid-stimulating hormone (tsh). also playing an important role in special situations are free thyroxine, an assessment of bound and unbound thyroid-binding globulin, trh stimulation, long-acting thyroid stimulator (lats), antibodies to thyroid hormone and ... | 1983 | 6426859 |
| [clinical application of tegafur suppositories for bladder tumor]. | a tegafur suppository of 750 mg was administered daily to 20 patients with bladder tumors, whose ages ranged from 43 to 84 years (average age 63.7). histological study revealed transitional cell papilloma in 6 cases, transitional cell carcinoma in 12 cases, squamous cell carcinoma in 1 case and malignant tumor with extensive necrosis in 1 case. the result of staging and grading was as follows: 8 cases of pta, 5 cases of pt1, 9 cases of pt2, 1 case of pt3a, 2 cases of pt3b and 1 case of t4; an, 6 ... | 1983 | 6426274 |
| [spironolactone in idiopathic hirsutism, clinical and biological evaluation of treatment]. | ten midly hirsute women with normal serum levels of testosterone were treated with spironolactone (100 mg die), and the effectiveness of this dose-schedule regimen was evaluated after six months of therapy. hirsutism subsided partly in all women. we estimated however, that in 6 of the 10 women, the clinical benefit was out weighed by the side effects and other drawbacks of a long term therapy and their medication was then stopped 6 months after its initiation. the changes induced on the pituitar ... | 1983 | 6430208 |
| different rates of thyrotropin suppression after total body scan in patients with thyroid cancer: effect of an optimal saturation regimen with thyroxine or triiodothyronine. | the rate of tsh suppression in patients with differentiated thyroid cancer, when therapy is re-started after total body scan, was investigated adopting an optimal saturation regimen, either with t4 or with t3. the first group of 6 patients received t4 as follows: from day 1 to 7 = 22, 11, 6, 4, 3.5, 3.2, 3.2 micrograms/day/kg body weight (b w) and continued with 3.2; the second group of 8 patients received t3 as follows: 2.4, 1.8, 1.4, 1.2, 1.1, 1.1 micrograms/day/kg bw and continued with 1.1. a ... | 1983 | 6423722 |
| alteration in thyroid hormone concentration during and after coronary bypass operation. | the effect of a surgical stress on serum tsh, t4, free t4, t3, rt3 and tbg was measured before, during and after a coronary bypass operation. precautions were taken to age, sex, pre-operative nutritional state, anamnestic illness, pre- and post-operative medication. compared to the pre-operative period tsh increased during operation and normalized at the first post-operative day, while only minor alterations for total and free serum t4 were observed. t3 and rt3 declined at the start of extracorp ... | 1983 | 6422832 |
| spermidine and spermine stimulate the activity of t4-dna ligase. | when the ability of t4-dna ligase from e. coli nm 989 to form higher molecular weight polymers from linearized plasmid pjdb 207 was followed, it was observed that physiological concentrations (0.5 to 1.0 mm) of spermidine and spermine greatly stimulated the formation of these polymers. the effect had a strict specificity since 1,3-diaminopropane, putrescine (1,4-diaminobutane) and n1-acetylspermidine neither stimulated nor inhibited this activity of dna ligase. the structural analogues of spermi ... | 1983 | 6362665 |
| [direct and indirect parameters for free thyroxine. ii. diagnostic accuracy in disorders of thyroid function and binding protein anomalies]. | part ii of the study concerning the clinical applicability of direct and indirect parameters for free thyroxine evaluates the diagnostic accuracy of the ft4-rias immophase, gammacoat, liquisol, amerlex and lisophase in relation to ft4 index and t4/tbg ratio. this comparison of methods is done on a thoroughly classified collection (n = 640) of patients with normal and impaired thyroid function including patients with binding protein anomalies (pregnancy, estrogen-medication, phenytoin therapy, re ... | 1983 | 6422443 |
| phase i pilot study on fast neutron teletherapy for advanced carcinomas of the head and neck region. final report on local control rate and survival. | a final report is presented on the local control rate and length of survival for 100 patients with advanced squamous cell carcinomas of the head and neck region who received fast neutron teletherapy at the university of washington during the period 1973 through 1977. sixty-two patients were treated with neutrons alone and 38 were treated with a combination of neutrons and photons as part of a mixed beam fractionation scheme. the overall initial complete remission rate was 68% for the mixed beam ... | 1983 | 6821811 |
| 125iodine implants as an adjuvant to surgery and external beam radiotherapy in the management of locally advanced head and neck cancer. | 125iodine seeds either individually placed or inserted into absorbable vicryl suture carriers were utilized in conjunction with surgery and external beam radiotherapy in an attempt to increase local control rates in patients with (1) advanced oropharyngeal and laryngopharyngeal cancers (t3-t4, n2-n3), (2) massive cervical lymphadenopathy (n3) and an unknown primary site and (3) locally recurrent head and neck cancers. forty-eight patients were treated with 55 implants. the carotid artery was imp ... | 1983 | 6821872 |
| t3 may be a better agent than t4 in the critically ill hypothyroid patient: evaluation of transport across the blood-brain barrier in a primate model. | thyroid hormone transport across the blood brain barrier in hypothyroid patients is clinically important yet poorly understood. to study this question, 200 micrograms of thyroxine (t4), 100 micrograms of 3,5,3'-triiodothyronine (t3) and 100 micrograms of 3,3',5'-triiodothyronine (reverse t3) were administered separately to 3 baboons, first iv and at a later date intrathecally (it). six animals were used. three received the iv injections and three received the it injections. in each of the 18 exp ... | 1983 | 6822088 |
| thyroxine 5'-deiodinase activity in brown adipose tissue. | homogenates of rat brown adipose tissue (bat) and epididymal fat pad (epi) catalyze the 5'-deiodination of 3,3',5'-triiodothyronine (rt3) in the presence of dithiothreitol. bat, but not epi, catalyzes the 5'-deiodination of thyroxine (t4) generating equal amounts of 3,5,3'-triiodothyronine (t3) and iodide. bat 5'-deiodinase activity was unaffected by propylthiouracil (ptu) and was elevated in hypothyroid rats, whereas epi 5'-deiodinase was totally inhibited by ptu and was depressed in hypothyroi ... | 1983 | 6822208 |
| hormonal control of growth in the infant rat: further evidence that neither thyrotropin nor thyroid hormones are transferred via milk to suckling pups. | experiments were conducted to obtain information on the possible transfer of tsh and thyroid hormones from mother rats to their infants via milk. the mothers were thyroidectomized by surgical or chemical (tapazole) means to eliminate maternal thyroid hormones from milk while increasing its content of tsh. their pups were either hypophysectomized (hx) or sham hx, and some pups were also chemically thyroidectomized (ctx) by tapazole injections. surgical thyroidectomy (stx) of the mothers reduced t ... | 1983 | 6822210 |
| a transplant system for studying hormonal control of growth of fetal rat tissues: effects of hypophysectomy growth hormone, prolactin, and thyroxine. | much uncertainty still exists about whether mammalian fetuses are dependent on and/or responsive to hormones that are growth promoting postnatally. to allow this problem to be investigated more meaningfully, a transplant system was developed. paws from 14- to 15-day-old fetal rats were incubated under the kidney capsules of 28- to 30-day-old syngeneic female hosts for up to 11 days. after an initial lag period, the growth rate of the transplants was similar to that of in situ paws. the implants ... | 1983 | 6822211 |
| thyroglobulin structure-function. the amino acid sequence surrounding thyroxine. | a 19-residue, thyroxine (t4)-containing peptide, tryp-t4, has been isolated from the tryptic digest of a low molecular weight, iodine-enriched fragment derived from 19s bovine thyroglobulin. this tryptic peptide represents the only site of significant iodination in the parent polypeptide fragment. the amino acid sequence of the tryptic peptide has been determined and is nh2-asn-ile-phe-glu-t4-gln-val-asp-ala-gln-pro-leu-arg-pro-cys-glu-leu-gln- arg-cooh. the carboxyl-terminal sequence of this pe ... | 1983 | 6822549 |
| plasma thyroglobulin measurements help determine the type of thyroid defect in congenital hypothyroidism. | plasma thyroglobulin (tg) measurements were conducted in infants with congenital hypothyroidism to determine their value in the classification of the thyroid defect. twenty hypothyroid patients were examined before 50 days of age. plasma t4, t3, tsh, and tg were measured and a thyroid scan was performed on all the infants. on the basis of clinical evaluation and the thyroid scans, patients were divided into three groups: group i, ectopic or eutopic hypoplastic glands (n = 11); group ii, goiters ... | 1983 | 6822634 |
| elevation of postmortem triiodothyronine in sudden infant death syndrome and in infants who died of other causes: a marker of previous health. | we measured serum concentrations of thyroxine (t4), triiodothyronine (t3), reverse t3, free t4, thyroid-stimulating hormone, and cortisol in 62 victims of sudden infant death syndrome (sids) in 30 infants who died of known causes and in 15 living controls. the mean t3 value was elevated in 69% of those with sids. 37% of the others who died, and in no control infants. after excluding those who died of known cause who had abnormal thyroid function (abnormal postmortem concentrations of t4, free t4 ... | 1983 | 6822923 |
| phenotypic and functional heterogeneity of human cloned natural killer cell lines. | extensive efforts have recently been made to characterize cells capable of mediating natural killing activity (see ref. 1 for review) and increasing evidence has arisen that these cells were heterogeneous. by using the methods we have recently developed for cloning natural killer (nk) cells derived from peripheral blood, we have analysed the heterogeneity of human nk cells. seven cell lines showing nk activity were established and studied for 4 months. their phenotype was determined with a serie ... | 1983 | 6823293 |
| bacteriophage phi w-14: the contribution of covalently bound putrescine to dna packing in the phage head. | bacteriophage phi w-14 is unusual because its dna contains 12 mol% of the hypermodified pyrimidine, alpha-putrescinylthymine. the phi w-14 virion is similar in morphology to t4, except that the phi w-14 head is isometric rather than prolate, there is no collar-whisker structure associated with the neck, the tail fibers are short (approximately 15 nm), and the base plate terminates in small plates or knobs rather than spikes. the contractile tail sheath of phi w-14 appears to have a right-handed ... | 1983 | 6823743 |
| low thoracic epidural anaesthesia for elective cholecystectomy in a patient with congenital heart disease and pulmonary hypertension. | a 52-year-old male with pulmonary hypertension secondary to partial anomalous pulmonary venous return unassociated with atrial septal defect was given thoracic epidural anaesthesia for elective cholecystectomy. partial anomalous pulmonary venous return is a rare congenital anomaly characterized by increased pulmonary blood flow which in severe cases results in pulmonary hypertension subjecting the right ventricle to strain. an epidural catheter was placed at the t11-t12 interspace and anaesthesi ... | 1983 | 6824989 |
| [calorimetric study of the conformational transformations of bacteriophage t4]. | 1983 | 6825550 | |
| specific antibodies and immunoglobulins in the oviductal fluid of the rhesus monkey. | levels of specific antibodies against model antigens, immunoglobulins g and a and also albumin, in oviductal fluid were studied in the rhesus monkey during the periovulatory period. animals were systemically or intravaginally immunized against t4 coliphages. attempts to induce ovulation were made with human menopausal gonadotropin (hmg) and human chorionic gonadotropin (hcg) monitored by radioimmunoassay of serum estrogen and progesterone. collection of tubal fluid over 6 to 13 days was accompli ... | 1983 | 6825870 |
| plasma thyroid hormones in cyclostomes: do they have a role in regulation of glycemic levels? | plasma levels of thyroxine (t4) and triiodothyronine (t3) were measured by radioimmunoassay in intact pacific lamprey (entosphenus tridentatus) undergoing the period of natural fasting and in pacific hagfish (eptatretus stouti) maintained unfed in the laboratory. plasma t3 levels in both lamprey and hagfish were always severalfold lower than t4 levels. the influence of thyroid hormones on glycemic level was studied following intraperitoneal injection of t4 or t3 (13-20 micrograms/100 g body wt), ... | 1983 | 6826052 |
| effects of excessive iodide administered in the dry period on thyroid function and health of dairy cows and their calves in the periparturient period. | experiments were conducted in three successive years in which iodide (i) doses of 1.25, 2.5, 5.0 and 7.5 mg/kg body weight were given to 47 dairy cows during the dry period, compared with 16 control cows on basal diets of 1 ppm i. effects on cows dosed at 1.25 and 2.5 mg i/kg (50 and 100 ppm dry feed) were not different from controls in terms of vitality of calves, changes in plasma thyroxine (t4), plasma triiodothyronine (t3), thyroxine secretion rate and 10-mo milk yields of the lactation afte ... | 1983 | 6826474 |
| the effects of the herbicide 2,4-dichlorophenyl-p-nitrophenyl ether (nit) on serum thyroid hormones in adult female mice. | the administration of the herbicide 2,4-dichlorophenyl-p-nitrophenyl ether (nitrofen; nit) to adult female mice by i.p. injection at doses of 500 and 1000 mg/kg/day for 3 days, reduced serum thyroxine (t4) levels. t4 levels were reduced in the high-dosage group by 60% of the control and by 20% in the low-dose group. serum triiodothyronine (t3) levels were relatively unaffected by nit treatment at either dose. the animals appeared euthyroid, as body weights were unaffected even though t4 levels w ... | 1983 | 6829046 |
| effects of oestradiol benzoate on the pituitary-thyroid axis of male and female rats. | in the present work the effects of oestradiol benzoate (eb) on pituitary and plasma concentrations of tsh, plasma t4 and t3, and thyroidal activity of male and female rats have been studied. wistar rats weighing between 150 to 200 g were injected sc with varying doses of eb in corn oil for 9 or 30 days. the animals were exsanguinated by cardiac puncture and the hypophyses removed and individually homogenized at 4 degrees c in 200 microliters pbs buffer. pituitary and plasma tsh were measured by ... | 1983 | 6829263 |
| lack of refractoriness to stimulation with long acting thyroid stimulator of thyroid hormone synthesis and thyroid hormone secretion in mice in vivo. | the present study was undertaken to examine whether long acting thyroid stimulator (lats) induces refractoriness of thyroid hormone synthesis and thyroid hormone secretion to the stimulator in vivo. male ddy mice fed with a low iodine diet and given 5 micrograms/ml of triiodothyronine (t3) in drinking water and libitum for 4 days were injected with 0.025 ml of lats positive serum (1000%/0.25 ml in rhe mckenzie bioassay) ip every 24 h for 9 days. groups of 5 mice were sacrificed before and 1, 3, ... | 1983 | 6829264 |
| applications of carbon dioxide laser surgery and radiation. a preliminary report. | ten patients with advanced or recurrent squamous cell carcinomas of the upper airway were treated with a combination of carbon dioxide laser surgery and radiation therapy to evaluate the tolerance to rapid sequencing of both modalities in a variety of clinical situations. other considerations were to accurately stage infiltrating tumors, to provide cytoreduction for t3 and t4 tumors, and to facilitate the optimal placement of intracavitary radium applicators. because of minimal postoperative pai ... | 1983 | 6830518 |
| the influence of environmental temperature, thyroid status and a synthetic oestrogen on the induction of fatty livers in chicks. | 1. hepatic lipid content, lipogenic enzyme activity and plasma lipid concentration were measured in chicks reared at 21 degrees or 34 degrees c and after thyroxine (t4), thiouracil (tu), propylthiouracil (ptu), dienestrol diacetate (dd) or ptu with dd had been given for 14 d. 2. at 34 degrees c there was a significant increase in the total liver lipid and triglyceride content. 3. injections of t4 decreased liver lipid content whereas it was increased by feeding ptu or dd. the effects of ptu were ... | 1983 | 6831278 |
| the value of combining radiotherapy with surgery in the treatment of hypopharyngeal and laryngeal cancers. | two-hundred and six cases of hypopharyngeal and laryngeal squamous cell carcinoma treated at the institut gustave-roussy were retrospectively analyzed. all of them were treated by surgery and they were divided into three therapeutic groups following the adjuvant radiotherapy: (a) postoperative radiotherapy at doses equal to or greater than 4500 rad; (b) postoperative radiotherapy at doses less than 4500 rad; and (c) preoperative irradiation at doses less than 4500 rad. group a included a greater ... | 1983 | 6831347 |
| capillary endothelial cell multiplication in adipose tissue pads on the thyroid during the feeding of thiouracil. | it has been reported that blood capillaries in adipose tissue pads on the upper and lower poles of the thyroid gland enlarge when fischer rats are fed thiouracil (tu) in a low iodine diet. to test whether the enlargement is accompanied by proliferation of the endothelial cells, [3h]thymidine was injected into rats fed the tu-containing diet, and labeling of the endothelial cells was studied by autoradiography. nuclear labeling of the capillary endothelial cells was observed in the mixed brown an ... | 1983 | 6832066 |
| conversion of thyroxine to 3,3',5'-triiodothyronine in the guinea pig placenta: in vivo studies. | studies of placental inner-ring deiodination of t4 were carried out in pregnant guinea pigs, by in situ placental perfusion. when [131i]t4 and [125i]rt3 were administered to the mother, the ratio of fetal side to maternal side [131i]rt3 was more than 10 times greater than the corresponding ratio for [125i]rt3. when radiolabeled t4 was supplied to the fetal side of the placenta in perfusion fluid, and the perfusate recycled through the placental circuit, there was a progressive increase in labele ... | 1983 | 6832069 |
| radiation therapy of carcinoma of the tonsillar region. | from 1960 through 1976, 353 consecutive patients with carcinoma of the tonsillar region were primarily treated with radiation therapy. the mean age of patients was 58 years with males predominating (78%). patients were classified according to a tnm and stage classification. most of the tumors were large, and the specific site of origin could not be determined in 33% of the patients. the predominant identifiable sites were the soft palate and uvula, 18%, the anterior tonsillar pillar, 18%, and th ... | 1983 | 6833015 |
| on the enzymatic basis for mutagenesis by manganese. | the effects of manganese on dna synthesis fidelity are measured using t4 dna polymerase. when the nucleotide analogue 2-aminopurine deoxyribonucleoside triphosphate competes against datp at thymine sites on template dna, the aminopurine misincorporation frequency increases from 6.3% in the presence of mg2+ to 29.2% in the presence of mn2+. the major cause of the increased error rate is an approximate 4-fold increase in the frequency of aminopurine misinsertions. exonucleolytic proofreading of am ... | 1983 | 6833210 |
| higher sensitivity of primary thyrotropin in screening for congenital hypothyroidism: a myth? | we have simultaneously measured t4 and tsh concentrations from 93,000 consecutive filter paper blood samples received by the quebec network for genetic medicine. t4 was measured using the micromedic automated system and tsh was measured by ria kits from pharmacia and becton dickinson, each for a 6-month period. statistical analyses to assess quality control parameters for the various methods revealed that the t4 assay had greater precision and reproducibility than either of the 2 commercially av ... | 1983 | 6833464 |
| failure of metoclopramide to affect thyrotropin concentration in the term human fetus. | metoclopramide (met), a potent dopamine receptor-blocking drug, or saline was administered to 125 term pregnant women at various time intervals (5-412 min) before delivery. maternal serum was obtained before and after met injection. cord blood was obtained at delivery in met-treated and saline-treated (control group) women. no significant changes in serum tsh, t4, t3, or rt3 concentrations were observed in maternal or cord blood after met administration. these results suggest that, in contrast t ... | 1983 | 6833467 |
| reduced bone mineral content in totally thyroidectomized patients: possible effect of calcitonin deficiency. | to further investigate the relationship between calcitonin deficiency and osteoporosis, we have measured bone mineral content (bmc) by single photon absorptiometry in patients made iatrogenically calcitonin deficient by prior total thyroidectomy for thyroid cancer. compared to sex-, age-, height-, and weight-matched normal controls, male patients had a significantly lower mean bmc at the midradius (1.162 +/- 0.02 vs. 1.301 +/- 0.05 g/cm; p less than 0.02) and the distal radius (1.180 +/- 0.04 vs ... | 1983 | 6833470 |
| cerebroside and sulfatide biosynthesis in the brain of snell dwarf mouse: effects of thyroxine and growth hormone in the early postnatal period. | snell dwarf mice (dw/dw) and normal mice (+/?) were injected with thyroxine (t4) (1 microgram/animal, four injections) and growth hormone (gh) (20 micrograms/animal, four injections) from the 5th to the 15th day of life. in the untreated dw/dw mouse brain, the specific activities of udp-galactose:ceramide galactosyltransferase (cgalt), paps:cerebroside sulfotransferase (cst), and 2', 3'-cyclic nucleotide 3'-phosphohydrolase (cnp) were decreased by 28, 25, and 37%, respectively, compared with the ... | 1983 | 6834036 |
| early intermediates in bacteriophage t4 dna replication and recombination. | we investigated, by density gradients and subsequent electron microscopy, vegetative t4 dna after single or multiple infection of escherichia coli with wild-type t4. our results can be summarized as follows. (i) after single infection (i.e., when early intermolecular recombination could not occur), most, if not all, t4 dna molecules initiated the first round of replication with a single loop. (ii) after multiple infection, recombinational intermediates containing label from both parents first ap ... | 1983 | 6834472 |
| the dissemination of cancer by transurethral resection of locally advanced prostate cancer. | in 1973 a study was done on 443 patients treated with radiation therapy for cancer of the prostate. an actuarial analysis was done on survival comparing patients whose cancer was diagnosed by transurethral resection of the prostate to those diagnosed by needle biopsy. this analysis indicated a doubling of recurrence and of deaths of patients diagnosed by transurethral resection of the prostate. this effect of transurethral resection of the prostate was observed in patients with t3 and t4 cancer ... | 1983 | 6834496 |
| initiation of erection and semen release by rectal probe electrostimulation (rpe). | instrumentation and methodology are described for rectal probe electrostimulation (rpe) in human males to elicit erection and allow semen collection. this system virtually eliminates shock hazard; the simultaneous monitoring of current, voltage and impedance ensures reliability and repeatability. it was tested with 8 neurologically intact subjects, and 12 paraplegic patients with lesions between t4 and l2. platinum electrodes delivered current (density never exceeding 0.37 ma per mm. at the elec ... | 1983 | 6834568 |
| [changes in thyroid hormone metabolism in subjects with liver cirrhosis]. | the behaviour of the serum levels of thyroxine (t4), 3,5,3' tri-iodothyronine (t3), tsh and of the free binding sites of the thyroid hormones binding globulins in 23 patients affected by hepatic cirrhosis, is described in function of the severity of the disease. the values obtained have been statistically compared to those of a group of 14 healthy subjects. a decrease of the serum levels of t3 directly related to the severity of the disease has been remarked in the cirrhotic patients, while no s ... | 1983 | 6835552 |
| thyrotoxicosis. clinical syndromes and laboratory diagnosis. | thyrotoxicosis is not one but many syndromes, and a variety of thyroid and nonthyroid disorders can clinically simulate thyrotoxicosis. thus, diagnosis is not always straightforward. heat intolerance and weight loss are hallmarks of the disorder. for correct identification, abnormal elevation of triiodothyronine (t3) or thyroxine (t4), specifically the free hormone or the calculated free hormone index, must be demonstrated. thyroidal uptake of iodine 123 helps to identify the etiology of the spe ... | 1983 | 6835885 |
| reversibility of ethylenethiourea-induced thyroid lesions. | groups of 50 male and 50 female sprague-dawley rats were fed diets containing 0, 75, 100, or 150 ppm ethylenethiourea (etu) for 7 weeks, at which time subgroups of 10 animals from each group were killed. subsequent subgroups of 10, 10, and 20 animals were killed after an additional 2, 3, and 4 weeks, respectively, on the control diet in order to examine whether the toxicological effects induced by etu were reversible. in both sexes, the mean body weight and feed consumption were significantly de ... | 1983 | 6836581 |
| [changes in thyroxine conversion (low t3 syndrome) in children at the time of their first manifestation of type i diabetes]. | measurements of t4, t3, rt3, and tsh were done in 27 children with newly diagnosed type i diabetes mellitus prior to institution of treatment. serum t4 concentrations were low in 18%, serum t3 concentrations were low in 37%. serum rt3 concentrations were elevated in 59%; the ratio rt3/t3 was elevated in 78%. the ratio rt3/t3 was significantly higher in the group of patients with the low ph, the low bicarbonate levels, the high blood glucose, and the high serum osmolality values than in the group ... | 1983 | 6837042 |
| hearing sensitivity in patients with myxoedema before and after treatment with l-thyroxine. | fifteen patients with confirmed myxoedema, median age 76 years, were consecutively referred for audiological evaluation. the diagnosis of myxoedema was based on the symptomatology, typical clinical appearance, increased tsh level and decreased t4. the audiological evaluation included routine ent-examination, pure-tone octave audiometry, determination of speech reception threshold and discrimination score. the function of the middle ear was evaluated by impedance audiometry, indicating both middl ... | 1983 | 6837285 |
| ultrastructural and functional effects of chronic endogenous stimulation of thyroid cells by thyrotropin. | following total thyroidectomy, a small quantity of thyroid tissue was transplanted to the spleen in order to study thyroid tissue subjected to chronically elevated levels of endogenous thyrotropin (tsh). plasma thyroxine (t4) and tsh levels were monitored and correlated with ultrastructural studies of the tissue over a 32-week experimental period. the effects of administration of an iodine-poor diet, and exogenous acute dose of tsh, and suppression of endogenous tsh through thyroxine administrat ... | 1983 | 6837480 |
| differences in iodinated peptides and thyroid hormone formation after chemical and thyroid peroxidase-catalyzed iodination of human thyroglobulin. | the distribution of iodine among the polypeptides of human goiter thyroglobulin (tg) was examined. tg was iodinated in vitro with 131i to levels of 2 to 84 gram atoms (g.a.)/mol using thyroid peroxidase (tpo) or a chemical iodination system. the samples were reduced, alkylated, and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. two low-molecular-weight peptides appeared preferentially in radioautograms of the sodium dodecyl sulfate (sds) gels of tpo-iodinated samples. io ... | 1983 | 6838223 |
| normal ranges of t4 screening values in low birthweight infants. | thyroxine (t4) screening values in infants of low birthweight in relation to birthweight and gestational age are reported. there were 86 healthy infants of low birthweight (group 1), and 29 preterm infants with respiratory distress syndrome (group 2). all the group 2 infants and 36% of those in group 1 had a t4 screening value below the cut-off point (-2.1 sd). in group 1 there was a significant increase in t4 with birthweight at a given gestational age, as well as with gestational age at a give ... | 1983 | 6838250 |
| identification and properties of myocardial myoglobin as a binder of iodothyronines. | gel filtration of dog myocardial cytosol previously incubated with [125i]t4 or [125i]t3 revealed hormone binding in three fractions, one of which, m-2, was presumptively identified as myoglobin by absorbance maximum, molecular weight and specific immunodiffusion. gel chromatography of purified horse or dog myoglobins incubated with labeled t3 or t4 resulted in coelution of the myoglobin and iodothyronine peaks. excess unlabeled thyroid hormone displaced no more than 25% of tracer bound to myoglo ... | 1983 | 6838502 |
| amino acid sequence of the unique 3,5,3'-triiodothyronine-containing sequence from porcine thyroglobulin. | a unique t3-containing peptide was isolated by chemical and enzyme treatments from ptgb of different iodine contents and microsequenced: leu-ala-ser-lys-ser-t3. this site can also form t4 to a small extent. it contains all the t3 of the protein and presents a special susceptibility to proteolysis thus suggesting a likely mechanism to explain that the thyroid secretes t3 preferentially to t4 in vivo. | 1983 | 6838607 |
| the chediak-higashi gene in humans. iii. studies on the mechanisms of nk impairment. | lymphocytes from six chediak-higashi (ch) patients were markedly depressed in their ability to lyse tumour cell targets in both 51cr release and single cell cytotoxicity assays. the frequency of lymphocytes bearing the okm1 marker and the frequency of t3+, t4+, t8+, ia+, mo1+, mo2+ and b1+ cells was normal among sheep erythrocyte rosetting (e+) and non-rosetting (e-) peripheral blood leucocytes analysed by flow cytofluorography. cells expressing the nk shared markers, okm1, mac-1, fcr, and the c ... | 1983 | 6839545 |
| role of thyroid in tissue respiration of birds. | thyroidectomy after 1 week, 1 month, and 13 months significantly depressed and low level of l-t4 significantly elevated the oxygen uptake of the liver and skeletal muscle of spotted munia and common myna. a higher dose of l-t4 had no effect on the rate of respiration. a reexamination of the published results on submammalian vertebrates together with those presented here suggests that an uniform pattern of response of skeletal muscle emerges if the values from thyroidectomized animals are compare ... | 1983 | 6840527 |
| a major role for bacteriophage t4 dna polymerase in frameshift mutagenesis. | t4 dna polymerase strongly influences the frequency and specificity of frameshift mutagenesis. fifteen of 19 temperature-sensitive alleles of the dna polymerase gene substantially influenced the reversion frequencies of frameshift mutations measured in the t4 rii genes. most polymerase mutants increased frameshift frequencies, but a few alleles (previously noted as antimutators for base substitution mutations) decreased the frequencies of certain frameshifts while increasing the frequencies of o ... | 1983 | 6840538 |
| acute and transient activation of pituitary-thyroid axis during unforced restriction in rats: component of nonshivering thermogenesis in conscious animals? | groups of 6-8 male wistar olac spf rats weighing about 300 g were subjected to unforced restriction (ur) in small cages with a metallic bottom and a plexiglas cover for various intervals from 2 min to 72 h. an acute activation of the pituitary-thyroid axis was found which was manifested by an increase of thyrotropin (tsh) and thyroxine (t4) levels at 2-5 min of ur. this was presumably due to the emotional effect of a rapid transfer and to the placing of the animals into restriction cages. later, ... | 1983 | 6840669 |
| the influence of the number of fractions and of overall treatment time on local control and late complication rate in squamous cell carcinoma of the larynx. | three hundred and ten patients with t3/t4, n0, m0 squamous cell carcinoma were irradiated with 200 kv x rays with total doses ranging from 4,900 to 6,200 rad, given in 21 to 35 fractions in 32-63 days. after a minimum follow-up period of 3 years, the local control rate was 50%; 21 severe late complications were observed among the patients. the dependence of local control rate and of late complication rate on the dose per fraction and on overall treatment time was analyzed by various statistical ... | 1983 | 6841183 |
| the primary treatment of advanced vocal cord cancer: laryngectomy or radiotherapy? | when different treatment modalities yield equal results in cancer treatment, the least mutilating is preferred. if results are different, however, the survival rate after treatment must be weighed against the quality of life. considerable controversy exists concerning the primary treatment modality for advanced glottic cancer, with some authors defending surgery (with or without radiotherapy) and others defending radiotherapy as sole treatment, with laryngectomy reversed for local failures. from ... | 1983 | 6841184 |
| the quality of life of patients following treatment for laryngeal cancer. | one hundred and twenty-nine patients have been interviewed 9 to 15 months following treatment for laryngeal cancer to determine the post treatment quality of voice and life. the patients were subdivided into 3 groups, successfully irradiated t1 and t2 patients, (89 patients), successfully irradiated t3 and t4 patients (24 patients) and those treated by surgery (16 patients). ninety-three percent of t1 and t2 patients and 79% of t3 and t4 patients are working following treatment as compared to 44 ... | 1983 | 6841185 |
| peripheral metabolism of thyroid hormones in man. i. direct measurement of the conversion rate of thyroxine to 3,5,3'-triiodothyronine (t3) and determination of the peripheral and thyroidal production of t3. | we describe here a new method for the direct measurement of the conversion rate of t4 to t3 in man. the metabolic study was performed in 23 subjects: 13 healthy controls, 7 t4-treated hypothyroids, and 3 sick euthyroid patients. the experimental protocol involved the simultaneous iv bolus injection of [125i]t4 and [131i]t3, and the use of sephadex g-25 column chromatography to determine the plasma concentrations of [125i]t4, [131i]t3, and [125i]t3 newly formed through 5'-monodeiodination of labe ... | 1983 | 6841556 |
| rapid pituitary and peripheral tissue responses to intravenous l-triiodothyronine in hypothyroidism. | acute cardiovascular, renal, pulmonary, metabolic, and pituitary responses to therapy of hypothyroidism with 25 micrograms iv t3 (group i g-i, n = 11) or 50 micrograms iv t3 (group ii, g-ii, n = 10)/day for 1 week have been studied. serum t3 levels were acutely normalized in both groups with the mean basal serum t3 levels (x +/- se) after 7 days, 98 +/- 10 micrograms/dl and 229 +/- 19 ng/dl, respectively. myocardial performance, noninvasively assessed by the pulse wave arrival time (qkd) and the ... | 1983 | 6841560 |
| abnormalities in the plasma concentrations of thyroxine, tri-iodothyronine and growth hormone in sex-linked dwarf and autosomal dwarf white leghorn domestic fowl (gallus domesticus). | plasma concentrations of gh were high in young birds (2-12 weeks old) and low in older birds (15 and 18 weeks old) in the control cornell k strain of white leghorn fowl. there was little variation in the plasma concentrations of tri-iodothyronine (t3) and thyroxine (t4) with age in this strain. similar patterns of plasma hormone concentrations with age were observed in autosomal recessive dwarf chickens. however, the plasma concentrations of t3 and t4 tended to be depressed while that of gh was ... | 1983 | 6842122 |
| summary of the third international conference on behçet's disease, tokyo, japan, october 23-24, 1981. | behçet's disease was associated with hla-b5, dr5, and mt2 antigens in japanese. no virus or other agent was clearly implicated. pathologic studies showed brainstem strokes and gut perforation from discrete ulcers. there was a significant reduction in t4 inducer-helper lymphocytes. immune complexes in serum were detected in 30 to 50% of patients, and antibodies to the glycolipid ga1 were found in 91% of patients with neurologic involvement. although several neutrophil functions were enhanced, for ... | 1983 | 6842475 |
| evidence that bacteriophage t4 eph1 is a missense hoc mutation. | an electrophoretic mutation of bacteriophage t4, eph1, appears to code for a missense hoc (highly antigenic outer capsid) protein. this is based on the observation that particles lacking hoc protein (hoc- particles), after incubation in a crude extract of escherichia coli infected with phage carrying the eph1 mutation acquired the electrophoretic mobility of the eph1 strain (the electrophoretic mobility of the eph1 strain itself is slower than that of hoc- particles). thus, it is likely that dur ... | 1983 | 6842681 |
| changes in myocardial cell size and number during the development and reversal of hyperthyroidism in neonatal rats. | increased workload was produced in newborn rats to study myocyte structure during conversion from hyperplastic to hypertrophic growth. newborn rats were given l-thyroxine (t4) for 12 days and killed at that time or 20 days after discontinuing t4 treatment. myocytes were isolated by a perfusion technique with collagenase. cellular dimensions were determined with a sonic digitizer. mean cell volumes were also determined with a coulter counter for comparison with values obtained with the digitizer. ... | 1983 | 6843089 |
| large granular lymphocytes in blood of healthy donors and patients with tumors. | the percentage of large granular lymphocytes (lgl) in the peripheral blood of patients with less advanced tumors (t1 and t2) averaged 2.15 +/- 1.3 as compared with 3.8 +/- 1.5 in patients with more progressive tumors (t3 and t4) and with metastases. the values of lgl remained constant as long as 6 weeks both in the diseased and healthy subjects and were not age- nor sex-related. this observation confirms the usefulness and reliability of the test as one of the markers of the immunologic state si ... | 1983 | 6843723 |
| [effect of alpha particles on bacteriophage t4]. | exponential survival curves were obtained for a dry film culture of bacteriophage t4 br+ after exposure to both alpha-particles and gamma-quanta. relative biological effectiveness of alpha-particles was 4.68 with respect to survival. the mutation spectrum after alpha-irradiation slightly differed from that produced by gamma-radiation. | 1983 | 6844561 |
| cytochemical analysis of human peripheral blood lymphocyte subsets defined by monoclonal antibodies. | human blood lymphocyte subpopulations, revealed by a panel of commercially available monoclonal antibodies by means of a rosetting technique, were submitted to direct cytochemical analysis and were shown to have distinguishing characteristics. t cells reactive with okt3 antibody (t3+) displayed higher beta-glucuronidase and dot-like alpha-naphthyl acetate acid esterase (anae) activity than t3- cells. helper/inducer cells (t4+) were characterized by a high level of dot-like anae activity, whereas ... | 1983 | 6844875 |
| effects of thyroxine supplementation on metabolic rate and aerobic capacity in a lizard. | effects of thyroxine (t4) on standard and maximal rates of o2 consumption and on citrate synthase activity, an index of mitochondrial oxidative capacity, were examined in the lizard dipsosaurus dorsalis. two weeks of daily injections of 200 ng t4/g body mass resulted in significant increases in standard (+60%) and maximal (+15%) rates of o2 consumption. citrate synthase activity in liver and in red iliofibularis and gastrocnemius muscles was significantly higher than in controls. correlational a ... | 1983 | 6846574 |
| a comparison of ajc and jjc proposals on tnm classification of maxillary sinus carcinoma. | the proposal of the jjc, established in 1977 in an effort to establish a tnm classification of maxillary sinus carcinoma, proved superior to the american joint committee for cancer staging and end results recording (ajc) proposal in terms of reproducibility, well-balanced distribution of cases of t, and prognostication using tnm as the indices, after a nationwide analysis of 474 n0m0 patients who were among the 541 patients first treated between 1970 and 1973 in 11 leading japanese medical insti ... | 1983 | 6847512 |
| the influence of methimazole on thyroglobulin-induced autoimmune thyroiditis in the rat. | experimental autoimmune thyroid disease was induced in august rats by immunization with rat thyroglobulin in complete freund's adjuvant. disease severity, assessed by thyroid histology and circulating levels of anti-tg antibody measured by an enzyme immunoassay, was maximal between 30 and 60 days after the initial immunization and thereafter waned. thyroid function through the duration of the disease, assessed by measurement of serum tsh levels by ria, remained normal. once the natural history o ... | 1983 | 6847827 |
| thyroid-stimulating hormone and thyroglobulin levels decrease with chronological age in children and adolescents. | tsh, thyroglobulin (tg), and the free t4 index were determined and related to chronological age in 24 normal boys (aged 7.6-17.6 yr; mean, 12.6 +/- 1.5 yr) and 20 normal girls (aged 7.8-17.0 yr; mean, 12.2 +/- 1.4 yr). there was no significant (p greater than 0.1) difference in mean serum tsh (3.06 +/- 2.07 vs. 2.97 +/- 2.07 microunits/ml) and tg levels (25.3 +/- 10.0 vs. 28.0 +/- 14.8 ng/ml) or free t4 index (9.6 +/- 1.6 vs. 9.4 +/- 1.3 micrograms/dl) between boys and girls. serum tsh levels co ... | 1983 | 6847868 |
| alterations in systolic time intervals in primary hypothyroidism as a consequence of warming. | the systolic time intervals (sti) of the preejection period, left ventricular ejection time and their ratio are influenced by thyroid function, the ratio increasing as thyroid secretion diminishes. we have previously shown that the majority of patients with primary hypothyroidism have a subnormal body temperature and that warming such individuals is accompanied by a fall in circulating levels of serum t4, t3, and tsh. on this occasion we studied 24 patients with primary hypothyroidism. fifteen o ... | 1983 | 6847870 |
| the influence of percutaneous fine needle aspiration on serum thyroglobulin. | to determine whether percutaneous needle aspiration of the thyroid affects tests of thyroid function, in particular thyroglobulin (tg), serum tg, t4, free t4 index, t3, and tsh were measured before and after percutaneous needle aspiration biopsy of the thyroid in 25 subjects. seven control subjects were tested before and after vigorous external manual palpation of the thyroid. serial measurements were made additionally in 3 subjects undergoing thyroid surgery to assess how quickly serum tg incre ... | 1983 | 6847872 |
| identification of thyroxine in human breast milk by gas chromatography-mass spectrometry. | in several previous publications it has been reported that human breast milk contains significant amounts of t4. great differences in concentrations were found by different authors. in all these publications t4 was assayed by ria, and it seems probable that the varying results obtained are due to varying specificity of the antibodies used in the assay. definite demonstration of the presence of t4 in breast milk requires a more specific method than ria. in the present work we have used combined g ... | 1983 | 6847873 |