Publications

role of ap endonuclease in dna breakage and cell inactivation of escherichia coli subjected to mild heat (52 degrees c).	the molecular mechanism of dna injury by mild heat was investigated using matched isogenic mutants of e. coli. on heating at 52 degrees c for 1 h, the number of dna single-strand breaks (ssbs) detected by the alkaline sucrose gradient sedimentation technique was consistently smaller in mutants nh5016 and bw2001, both deficient in the ap (apurinic/apyrimidinic) endonuclease of exonuclease iii, as compared with their wild-type parent ab1157. the greater number of ssbs in the wild type was accompan ...	1983	6338352
evidence for pineal gland modulation of the neuroendocrine-thyroid axis.	experiments with rats and hamsters have provided evidence for an inhibitory action of the pineal gland on the neuroendocrine-thyroid axis. while maintenance of these animals in short photoperiod results in reduced levels of circulating thyroxin (t4), pinealectomy restores the levels to normal. recent studies suggest that an active pineal gland produces a substance which inhibits thyrotrophin-releasing hormone release from the hypothalamus. several investigators have concluded that endogenous mel ...	1983	6338409
yeast 2-microns plasmid dna replication in vitro: purification of the cdc8 gene product by complementation assay.	extracts of the yeast saccharomyces cerevisiae support dna replication on exogenous yeast 2-microns plasmid dna templates. a crude extract from a s. cerevisiae cell division cycle mutant, cdc8-1, expressed the temperature-sensitive phenotype since it could be inactivated at 42 degrees c in vitro. this heat-inactivated extract was fully complemented by the addition of either wild-type or cdc8-1 single-stranded dna binding protein (ssb). restoration by ssb of the activity of the mutant cell extrac ...	1983	6338494
histiocytosis-x: in situ characterization of cutaneous infiltrates with monoclonal antibodies.	cutaneous lesions in three cases of histiocytosis-x were studied by light microscopy, electron microscopy, and immunoperoxidase technics. in each case, birbeck granule-containing histiocytosis-x cells infiltrated the epidermis and were apposed to lymphocytes. the histiocytosis-x cells and normal langerhans cells stained with anti-t6 and anti-i1 (ia-like) antibodies but were negative with anti-t3, anti-t8, anti-m1, and anti-lysozyme antibodies. in addition, the histiocytosis-x cells also stained ...	1983	6338698
monoclonal antibody characterization of surface antigens in childhood t-cell lymphoid malignancies.	although childhood t-cell acute lymphocytic leukemia (t-all) and t-cell non-hodgkin's lymphoma (t-nhl) have certain clinical features in common, t-all carries a notably poorer prognosis than does t-nhl. to determine whether the malignant cells from patients with these disorders are distinguishable, we examined bone marrow and/or blood from 51 children with t-all and tumor biopsy specimens from 17 with t-nhl, using a panel of monoclonal antibodies directed against t-cell differentiation antigens. ...	1983	6338974
dipeptide formation with misacylated trnaphes.	several misacylated p-site trnas have been prepared by the t4 rna ligase-mediated coupling of escherichia coli trnaphe missing the 3'-terminal cytidine and adenosine moieties and n-acetylaminoacyl-pcpa derivatives. the reaction proceeded in reasonable yield in each case and the trna products were purified conveniently by successive chromatographies on deae-cellulose and benzoylated deae-cellulose. the misacylated trnaphes were assayed for participation in the peptidyltransferase reaction, using ...	1983	6339501
mechanism of ribosome frameshifting during translation of the genetic code.	some frameshift mutations are strongly suppressed by limitation for particular aminoacyl-trna species. here, we show that ribosome frameshifting at a specific tryptophan codon during trp-trna limitation accounts for suppression of a group of downstream frameshift alleles in the riib gene of bacteriophage t4. genetic and physiological observations strongly suggest that ribosome frameshifting at this position depends on the binding of a noncognate (leucine) trna.	1983	6339944
stabilization of proteins by a bacteriophage t4 gene cloned in escherichia coli.	the cloned bacteriophage t4 pin gene functions to stabilize several different kinds of proteins in escherichia coli bacteria. incomplete proteins such as puromycyl polypeptides, abnormal but complete proteins such as the lambda phage tso protein, and labile eukaryotic proteins encoded by genes cloned in e. coli such as mature human fibroblast interferon are stabilized in cells in which the t4 pin gene is expressed. the cloned t4 pin gene does not seem to affect the turnover of normal e. coli pro ...	1983	6340113
[lethal and mutagenic action of solar radiation on model microbiological test systems].	lethal, mutagenic and recombonogenic action of the solar radiation on the model microorganisms--phage t4, bacteria escherichia coli and ascomycet aspergillus nidulans--has been studied. a considerable lethal effect of the solar radiation on phage t4 and e. coli was found. an increasing of mutation frequency in e. coli and a. nidulans by sunlight was also revealed. recombinogenic action of solar radiation has been demonstrated in the experiments with diploid a. nidulans strains. it was shown that ...	1983	6340743
dna injection during bacteriophage t4 infection of escherichia coli.	the process of phage t4 dna injection into the host cell was studied under a fluorescent microscope, using 4',6-diamidino-2-phenylindole as a dna-specific fluorochrome. the phage dna injection was observed when spheroplasts were infected with the artificially contracted phage particles having a protruding core. the dna injection was mediated by the interaction of the core tip with the cytoplasmic membrane of the spheroplast. a membrane potential was not required for the process of dna injection. ...	1983	6341365
the mechanism of recovery of hepatic t4-5'-deiodinase during glucose-refeeding: role of glucagon and insulin.		1983	6341769
csf cells in multiple sclerosis: monoclonal antibody analysis and relationship to peripheral blood t-cell subsets.	mononuclear cells were analyzed in csf and blood of 102 patients with ms. in csf, the majority (78%) of cells were t lymphocytes (t3+), and the ratio of inducer (t4+) to suppressor/cytotoxic (t8+) cells was 2:1. no characteristic alterations in csf phenotypes could be related to changes in circulating t8 cells or to disease activity. in a group of 75 patients, csf cell count was higher in patients with low numbers of circulating t8 cells than in those with normal t8 cells. thus, decreases in sup ...	1983	6341872
the endocrine manifestations of hemochromatosis.	we have evaluated the endocrine changes in 10 male subjects with hemochromatosis. two subjects initially had aplastic anemia, and the remainder had idiopathic hemochromatosis. four of the ten patients had diabetes mellitus. sexual dysfunction (impotence and/or decreased libido) was observed in 8 subjects. six patients had subnormal testosterone levels; fsh levels were almost uniformly low, but lh concentrations were more variable. only three patients had normal testosterone responses to hcg. hyp ...	1983	6342390
immunoperoxidase localization of lymphocyte subsets in the host response to melanoma and nevi.	using an avidin:biotin immunoperoxidase system with monoclonal antibodies to lymphocyte subsets, we have investigated the host response to malignant melanoma and melanocytic nevi in frozen sections. eight primary melanomas, eight metastases, three dysplastic nevi, and two dermal nevi were studied with antibodies t11, t4, t8, and b1. sections were read in a semiquantitative manner by two observers. virtually all lymphocytes in these lesions were t-cells (t11 positive). in all primary melanomas, i ...	1983	6342758
thyroid hormone regulation of glucose homeostasis in the miniature pig.	the effect of different thyroid states on glucose homeostasis was investigated during metabolic adaptation to starvation in the conscious unrestrained miniature pig. moderate hyperthyroidism increased the rate of glucose turnover, whereas hypothyroidism was without effect. glucose recycling was elevated in hyperthyroid pigs, and reduced after thyroidectomy. supplementary doses of t4 normalized total glucose recycling. glucose metabolic clearance rate and pool size were unaffected by thyroid horm ...	1983	6343057
treatment of locoregionally advanced breast cancer with surgery, radiotherapy, and combination chemoimmunotherapy.	fifty-two patients with locally advanced primary breast cancer (t3, t4, n2, n3) but no evidence of distant metastases were treated with three cycles of combination chemotherapy. the regimen consisted of 5-fluorouracil, adriamycin, cyclophosphamide, and bacillus calmette-guerin (fac-bcg), followed by local therapy (simple mastectomy and/or radiotherapy to the breast/chest wall and the regional lymphatic system) and adjuvant chemotherapy for two full years. the results were compared with those in ...	1983	6343312
properties of bacteriophage t4 ribonucleoside diphosphate reductase subunits coded by nrda and nrdb mutants.	as a part of the study of the bacteriophage t4-induced deoxyribonucleotide synthetase complex, an investigation has been made of the t4 ribonucleoside diphosphate reductases formed by a series of mutants of nrda and b, the genes coding, respectively, for the alpha 2 and beta 2 subunits of the enzyme. datp affinity columns were used to isolate the enzyme by a single-step procedure. the molecular weights of the alpha and beta chains have been found to be 84,000 and 43,500, respectively, by sodium ...	1983	6343369
ribonuclease bn: identification and partial characterization of a new trna processing enzyme.	a new ribonuclease, rnase bn, has been identified and partially purified from a strain of escherichia coli lacking rnase ii and rnase d by using the artificial trna precursor trna-c-[14c]u as substrate. this enzyme is present in e. coli b but absent from the trna processing mutant strain bn which is unable to process extraneous 3' residues on certain phage t4-specified trna precursors. the properties of rnase bn clearly distinguish this enzyme from other known e. coli exoribonucleases. it is opt ...	1983	6344080
immunohistochemical localization of thyroxine (t4) and triiodothyronine (t3) in the rat thyroid gland under various experimental conditions.	detection of t4 and t3 in paraffin sections of the rat thyroid gland in various functional states was attempted by an immunoenzyme technique. both hormones showed a similar localization, being detected mainly in the colloid in the follicular lumen, and sometimes in certain regions of the cytoplasm of the follicular epithelium. following administration of tsh, their stainability was increased, and the localization within the cytoplasm became more remarkable. decrease or disappearance of stainabil ...	1983	6344530
purification and some properties of bacteriophage t4 particle-associated lysozyme.	bacteriophage t4 particle-associated lysozyme, purified to electrophoretic homogeneity, was found to be a protein with a relative molecular mass of 15000. the lysozyme was purified from the particles of bacteriophage t4 e mutant and from the lysates of the 5tsl e t4 mutant, in which the enzyme is in soluble form. in the purification procedure advantage was taken of the affinity of the enzyme for glcnac-murnac-lala-dglu-msa2pm-dala (c6 muropeptide), one of the products of the digestion of escheri ...	1983	6345157
involvement of gene 49 in recombination of bacteriophage t4.	the role of t4 gene 49 in recombination was investigated using its conditional-lethal amber (am) and temperature-sensitive (ts) mutants. when measured in genetic tests, defects in gene 49 produced a recombination-deficient phenotype. however, dna synthesized in cells infected with a ts mutant (tsc9) at a nonpermissive temperature appeared to be in a recombinogenic state: after restitution of gene function by shifting to a permissive temperature, the recombinant frequency among progeny increased ...	1983	6345264
binding of yeast trnaphe anticodon arm to escherichia coli 30 s ribosomes.	a 15-nucleotide fragment of rna having the sequence of the anticodon arm of yeast trnaphe was constructed using t4 rna ligase. the stoichiometry and binding constant of this oligomer to poly(u)-programmed 30 s ribosomes was found to be identical to that of deacylated trnaphe. the anticodon arm and trnaphe also compete for the same binding site on the ribosome. these data indicate that the interaction of trnaphe with poly(u)-programmed 30 s ribosomes is primarily a result of contacts in the antic ...	1983	6345793
effects of uv irradiation on the fate of 5-bromodeoxyuridine-substituted bacteriophage t4 dna.	we have carried out a series of experiments designed to characterize the impact of uv irradiation (260 nm) on 5-bromodeoxyuridine-labeled (heavy) t4 bacteriophage, both before and after infection of escherichia coli. in many respects, these effects differ greatly from those previously described for non-density-labeled (light) phage. moreover, our results have led us to propose a model for a novel mechanism of host-mediated repair synthesis, in which excision of uv-damaged areas is followed by in ...	1983	6345803
circular permutation analysis of phage t4 dna by electron microscopy.	phage t4 is known to have a linear duplex chromosome that is circularly permuted and terminally repeated. we found, by denaturation and self-reannealing experiments, that circular permutation in t4 native dna is not random. their multimodal distribution of permutation is compatible with the "headful packaging" model with the additional specifications that the encapsulation of dna starts at several sites and these are not random distributed.	1983	6346725
inflammatory cells in sarcoid granulomas detected by monoclonal antibodies and an esterase technique.	inflammatory cells in situ in kveim reaction papules were identified in 15 patients with avidin-biotin-peroxidase complex and biotin-avidin-peroxidase methods for surface epitopes and with a simultaneously capturing azo dye method for cytoplasmic acid alpha-naphthyl acetate esterase (anae). the spatial relationship of cells in granulomas indicates a concentric arrangement. endogenous peroxidase-negative, anae+, but okia-negative immunoincompetent epithelioid cells were in the center. t3+, anae+ ...	1983	6347482
processing of bacteriophage t4 trnas: a precursor of species 1 rna.	a precursor molecule of species 1 rna, p2sp1, that accumulates when an rne (rnase e-) mutant is infected with a t4 deletion mutant (delta 27) is also found after infection of an rne host mutant by different deletion mutants or wild type bacteriophage t4. low levels of this molecule were also found in a wild-type host infected with a wild-type t4. this precursor molecule accumulates at higher concentrations at 43 degrees c as compared to 30 degrees c or 37 degrees c. structural analysis of the pr ...	1983	6347717
chronic effects of amiodarone in patients with refractory ventricular tachycardia.	we examined the chronic electrophysiologic, systemic, and pharmacologic effects of chronic oral amiodarone therapy in 24 patients with refractory ventricular tachycardia and organic heart disease. chronic amiodarone therapy resulted in significant increases in r-r interval (from 798 +/- 182 msec to 912 +/- 100 msec; p less than 0.01), p-r interval (from 205 +/- 66 msec to 221 +/- 87 msec; p less than 0.02), qrs duration (from 103 +/- 24 msec to 115 +/- 28 msec; p less than 0.001), and q-tc inter ...	1983	6347914
formation of 5-hydroxymethylcytosine-containing pyrimidine dimers in uv-irradiated bacteriophage t4 dna.		1983	6348807
amino acid sequence around a hormonogenic tyrosine residue in the n-terminal region of human thyroglobulin after in vivo and in vitro iodination.	reduced and s-alkylated human thyroglobulin (htgb), normally iodinated, was previously shown by sds/page to contain a small peptide (mr 26 000) rich in thyroxine. this peptide was not found when very poorly iodinated htgb was treated under the same conditions but was present after iodination in vitro. peptide 26 k was purified from in vivo and in vitro iodinated htgb. the study of these peptides shows that: 1/ at the iodination levels studied (0.18 and 0.25% iodine) one of the preferential hormo ...	1983	6349629
[plasma concentrations of thyroid hormones in the domestic goat. seasonal variations in relation to age].	plasma thyroid hormones (t3 and t4) were investigated by immunoenzymatic methods in an umbrian indigenous goat population. seventy females and 10 males, aging 1 to 14 years, were tested in different periods of the year. mean values (m +/- sd) of 320 determinations were 2,4 +/- 1,5 ng/ml (ranging from 0,5 to 7,0) for t3 and 6,5 +/- 2,5 micrograms/dl (range 2,2 - 14,2) for t4. the highest values for both hormones were recorded in spring, the lowest at the end of the summer (mean decrease: t3 = 57% ...	1983	6349664
immunological markers of human mononuclear cells.	the immune response to an antigenic challenge results from complex intercellular communications between several populations of lymphoreticular cells. immunocompetent cells are derived from a common stem cell precursor and develop along two distinct pathways. the t-cell lineage (t-lymphocytes) originates in the thymus and mediates cellular immunity, whereas b-cells (bone marrow derived lymphocytes) mature into antibody producing cells. the maturation of b-cells into plasmacytes is under the contr ...	1983	6349849
regulation of the immune response in plasmodium falciparum malaria. i. non-specific proliferative responses in vitro and characterization of lymphocytes.	the mitogen-induced dna synthesis in vitro in lymphocytes from 20 patients acutely ill with plasmodium falciparum malaria was compared with that of 16 healthy donors. within both groups part of the donors were individuals who had only experienced short exposure or none at all to the parasite (sweden) while the other part were donors living in a malaria endemic area (colombia). the proliferative response to the t cell mitogen la (leucoagglutinin from pha) of the patients was significantly reduced ...	1983	6349879
use of monoclonal antibodies in a study of the development of t lymphocytes in the human fetus.	a panel of monoclonal antibodies (okt3, 4, 6, 8, 10, 11) was used for the identification of t lymphocyte subpopulations in cell suspensions of human fetal liver, thymus, bone marrow and spleen. in liver suspensions of 8-16 week old fetuses and in bone marrow suspensions (12-20 weeks) less than 5% of lymphocytes reacted with either okt3, 11, 4, 8 or 6, whereas the okt10 antibody bound to, respectively, 35 and 86% of lymphocytes in these tissues. in liver suspensions of 17-20 week old fetuses, abo ...	1983	6349881
cellular infiltrate in situ and response kinetics of human intradermal and epicutaneous tuberculin reactions.	monoclonal antibodies used in the avidin-biotin-peroxidase complex (abc) method and a histochemical azo-dye method for acid alpha-naphthyl acetate esterase (anae) were used to identify t lymphocytes and their functional subpopulations, b cells, and mononuclear phagocytes in tuberculin-test reactions. at 6 and 24 hr up to 52% of all dermal inflammatory cells in situ were t6+, whereas at 72 hr no t6+ cells were observed in the dermis. this suggests that in the initial phases of tuberculin reaction ...	1983	6349886
dissociation between coupled lymphocyte phenotypic and functional properties in sézary cells.	neoplastic lymphocytes derived from three patients suffering from sézary syndrome were studied by immunologic and functional analysis. populations of neoplastic t cells (greater than 90% as judged by morphologic criteria) were isolated from involved lymph nodes. sézary cells expressed t3 and t4 but not t8 membrane antigens displaying the same phenotypic pattern of normal helper/inducer t cells. on the contrary the mitogenic response of sézary cells to phytohemagglutinin appears lower than that e ...	1983	6349888
r plasmid dihydrofolate reductase with a dimeric subunit structure.	dihydrofolate reductase specified by plasmid r483 from a trimethoprim-resistant strain of escherichia coli has been purified 2,000-fold to homogeneity using dye-ligand chromatography, gel filtration, and polyacrylamide gel electrophoresis. the protein migrated as a single band on nondenaturing polyacrylamide gel electrophoresis and had a specific activity of 250 mumol/mg min(-1). the molecular weight was estimated to be 32,000 by gel filtration and 39,000 by ferguson analysis of polyacrylamide g ...	1983	6350298
macromolecular crowding allows blunt-end ligation by dna ligases from rat liver or escherichia coli.	in the presence of high concentrations of any of several types of macromolecules, dna ligase preparations from rat liver nuclei or from escherichia coli actively catalyze the blunt-end ligation of dna. this is in contrast to the lack of activity on such substrates by these enzymes under conventional assay conditions. in addition, the previously established activity of t4 dna ligase on blunt-ended molecules is greatly increased in the presence of high concentrations of macromolecules. because suc ...	1983	6351067
a structural role for dihydropteroyl hexaglutamate in the tail baseplate of various bacteriophages.	a novel non-metabolic role is proposed for dihydropteroyl hexaglutamate as a critical link binding together sub-structures of the tail of escherichia coli bacteriophage t4. six molecules of this folate compound have been found to be components of the complex tail baseplate of the phage particle. the baseplate is assembled using a total of at least 18 viral gene products in a series of reactions in which six wedge-like elements (each 0.7 x 10(6) daltons) bind symmetrically around a central tail p ...	1983	6351553
interplay among processing and degradative enzymes and a precursor ribonucleic acid in the selective maturation and maintenance of ribonucleic acid molecules.	in order to understand why the first trna (trnagln) in the t4 trna gene cluster is not produced when t4 infects an rnase iii- mutant of escherichia coli, rna metabolism was analyzed in rnase iii- rnase p- (rnc, rnp) cells infected with bacteriophage t4. after such an infection a new dimeric precursor rna molecule of trnagln and trnaleu has been identified and analyzed. this molecule is structurally very similar to k band rna that accumulates in rnc+ rnp strains. it is four nucleotides shorter th ...	1983	6351914
t cell regeneration after allogeneic bone marrow transplantation.	various t cell subsets were characterized by double immunofluorescent staining using monoclonal antibodies (moab) in blood, bone marrow (bm) and tissues of 29 patients after allogeneic bm transplantation (bmt). in an attempt to prevent graft versus host disease (gvhd), 15 patients received cyclosporin a (cy a). in the remaining 14 patients the bm was pre-incubated with a moab, okt3. the regeneration of t4+ subset was delayed and the level of t8+ cells was abnormally high even 1 year after engraf ...	1983	6352107
the primary structure of escherichia coli glutaredoxin. distant homology with thioredoxins in a superfamily of small proteins with a redox-active cystine disulfide/cysteine dithiol.	an immunosorbent method using antiglutaredoxin-sepharose was developed for purification of glutaredoxin in high yield from a mutant strain of escherichia coli k 12 lacking thioredoxin reductase (c 10-17). the primary structure of the protein was determined by analyses of [14c]carboxymethylated glutaredoxin and its proteolytic fragments obtained by digestions with trypsin, clostripain, chymotrypsin and staphylococcal glu-specific extracellular protease. the single active-center disulfide has the ...	1983	6352262
simultaneous enzyme-immunocytochemical detection of antigens in monocellular specimens with monoclonal antibodies.	an immunoperoxidase and immunoalkaline phosphatase technique is described specially devised for separate cell specimens such as smears, cytospins, and tissue imprints. the reaction may be used alone or in combination for simultaneous staining with 2 different monoclonal antibodies. monoclonal antibodies leu3a, leu2a, t4, t8, anti-kappa, and anti-lambda light chain were used in combination to visualize subsets of human lymphocytes selectively. six cases of chronic lymphocytic leukemia were classi ...	1983	6352820
immunoelectron microscopic study of the distribution of t cell subsets in rheumatoid synovium.	the perivascular mononuclear cell collections of the rheumatoid synovium were examined both at the light and electron microscopic level by an immunoperoxidase staining technique using monoclonal antibodies directed against t cell subsets. these accumulations were variable in composition and size, not only in specimens from different patients but in the same specimen. some areas (lymphocyte-rich areas) contained mainly small lymphocytes in clusters and others (transitional areas) contained blast ...	1983	6352849
altered colonizing ability for mouse large intestine of a surface mutant of a human faecal isolate of escherichia coli.	escherichia coli f-17 sr a human faecal isolate, is resistant to the t-series of bacteriophages (i.e. t2 to t7). a t2-sensitive mutant of e. coli f-17 sr was isolated following acriflavin treatment. this mutant, e. coli f-17 sr ts was found to be sensitive to the entire t-series of phages. e. coli f-17 sr and e. coli f-17 sr ts did not differ quantitatively in total lps content. however, analysis of lps revealed that a large fraction of e. coli f-17 sr ts was devoid of o-side-chains. this accoun ...	1983	6352858
measurement of repair patch size by quantitation of nucleotides excised during dna repair in vivo.	escherichia coli uvr- cells, prelabeled in their dna, were infected with phage t4 denv+ or t4 denv- under conditions that preclude phage-mediated degradation of the bacterial chromosome. measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of ...	1983	6352957
expression of cloned bacteriophage t4 uvsw and uvsy genes in rec+ and rec- escherichia coli.	chimeric plasmids containing the uvsy uvsw region of the t4 genome were examined for the expression of these genes. certain of these plasmids were shown to express the uvsy or the uvsw gene products by their ability to complement the uv sensitivity of infecting uvsw or uvsy mutant phage. also, a chimeric plasmid containing both the uvsw and uvsy genes increases the survival of uv-irradiated, methyl methane sulfonate- or ethyl methane sulfonate-treated reca hosts.	1983	6352958
acid protease activity in thyroid gland from patients with graves' disease.	although the activity of lysosomal protease in graves' thyroid is considered to be increased, there has been no quantitative method to estimate the protease activity in the thyroid tissue due to the contamination of thyroglobulin (tg) which varies in susceptibility to the protease. in the present study, the proteolytic activity (pa) of thyroid lysosomal protease preparation (p25) separated from tg was assayed using 125i labeled rat tg. more than 95% of 125i-tg was hydrolyzed at ph 4.0 without de ...	1983	6353140
using t4 rna ligase with dna substrates.		1983	6353144
[time structure of endocrine secretion. ii. circannual variations in the free fractions of tri- and tetraiodothyronine, cortisol, human growth hormone and plasma insulin in healthy subjects].	circadian and circannual variations of several endocrine activities and their biological implications in health and diseases were described in men. aim of our work was to perform a further observation on circannual bioperiodicities of some hormone: free fractions of t3 and t4, cortisol, hgh, pth and serum insulin. four healthy young males volunteered to be submitted to the study from june and their blood-samples collected six times a day, every other month, in the course of one year. our data we ...	1983	6354214
insertion of nucleotides opposite apurinic/apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: uniqueness of adenine nucleotides.	m13 dna containing 20-30 apurinic/apyrimidinic (ap) sites per intact circular molecule was prepared by growing phage on an ung- dut- escherichia coli mutant and treating the dna with uracil n-glycosylase. ap sites obstruct in vitro dna synthesis catalyzed by e. coli pol i. the position at which termination of synthesis occurs was determined for four enzymes. t4 dna polymerase terminates one nucleotide before putative ap sites. dna pol i, amv reverse transcriptase, and dna polymerase alpha termin ...	1983	6354260
identification and biosynthesis of the bacteriophage t4 mot regulatory protein.	the t4 mot gene regulates middle mode rna synthesis in phage-infected cells. the mot gene product has been identified in two ways. (i) infections with amber and temperature-sensitive mot mutants both lead to the disappearance of a number of protein bands on sds-polyacrylamide gels. these are middle mode proteins whose synthesis depends on mot function. the mot protein disappears from such gels after infection with a mot amber mutant, but not with the mot missense mutant. (ii) this same protein i ...	1983	6354709
glucagon does not modulate the alterations in t3 metabolism consequent to dietary manipulation and diabetes.	low serum t3 levels and hyperglucagonemia are characteristic features of a number of catabolic states such as fasting and uncontrolled diabetes. the present study was performed to elucidate the relationship between this hyperglucagonemia and t3 metabolism. serum glucagon and t3 and hepatic t4-5'-deiodinase activity (t4 leads to t3) were examined in groups of rats (t4-treated) fed (chow versus carbohydrate), fasted, or diabetic (streptozotocin 100 mg/kg i.p.) for 48-72 h. in the carbohydrate-fed ...	1983	6354787
ontogeny of embryonic chicken lung: effects of pituitary gland, corticosterone, and other hormones upon pulmonary growth and synthesis of surfactant phospholipids.	the actions of hormones on growth, cellular proliferation, and on synthetic rates of the major surfactant phospholipids, phosphatidylcholine (pc) and disaturated pc (dspc), were studied in the lung of the chick embryo. particular emphasis was placed on the effects of hypophysectomy, pituitary transplantation, and treatment with corticosterone (cort). one study was concerned with hydrocortisone (hycort), estrogen (e2), thyroxine (t4), ovine prolactin (oprl), and insulin. hypophysectomy interfered ...	1983	6354838
effects of storage times and temperatures on t3, t4, lh, prolactin, insulin, cortisol and progesterone concentrations in blood samples from cows.	little is known about stability of hormones in blood samples stored under various conditions. this study was conducted to examine stability of triiodothyronine (t3), thyroxine (t4), luteinizing hormone (lh), prolactin, insulin, cortisol and progesterone in blood and serum samples. experiment 1 was designed to determine if concentrations of these hormones were affected by exposure to cellular elements of anticoagulated and coagulated blood when stored at 4 c and room temperature (22 to 26 c). jug ...	1983	6355041
products of bacteriophage t4 genes 32 and 45 improve the accuracy of dna replication in vitro.	the six "accessory" proteins of the bacteriophage t4 specified by replication genes 32, 41, 44, 45, 61, and 62 were studied for their ability to enhance the accuracy with which phage t4 dna polymerase (product of gene 43) replicates synthetic homopolymer duplexes in vitro. two of these proteins, gene 32-protein (helix-destabilizing protein) and gene 45-protein, inhibited the selection of incorrect, but not correct, precursors, at the growing strand end. gene 32-protein is shown to enhance replic ...	1983	6355084
fate of cloned bacteriophage t4 dna after phage t4 infection of clone-bearing cells.	plasmid pbr322 replication is inhibited after bacteriophage t4 infection. if no t4 dna had been cloned into this plasmid vector, the kinetics of inhibition are similar to those observed for the inhibition of escherichia coli chromosomal dna. however, if t4 dna has been cloned into pbr322, plasmid dna synthesis is initially inhibited but then resumes approximately at the time that phage dna replication begins. the t4 insert-dependent synthesis of pbr322 dna is not observed if the infecting phage ...	1983	6355485
incorporation of thymine-containing dna precursors in wild-type and mutant t4-infected plasmolysed cells.	t4-infected cells, plasmolysed 15 min after infection, incorporate low concentrations (less than 20 microm) of deoxythymidine (tdr) into dna at a significantly greater rate than dtmp, dttp or thymine. at higher concentrations (greater than 40 microm), dtmp incorporation rate is high, approaching that of tdr at 200 microm. tdr is selectively incorporated at all concentrations tested, and is not inhibited by the other thymine containing dna precursors. incorporation of low concentrations of tdr re ...	1983	6355761
incorporation of thymine-containing dna precursors in plasmolysed cells infected by the t4 non-lethal recombination defective mutants.	incorporation of tdr is aberrant in cells plasmolysed 15 min after infection by the recombination defective t4 chi and omega mutants. the in situ results parallel those obtained in vivo: at high tdr concentrations both t4 chi and t4 omega induced incorporation is slightly reduced compared to wild type, whereas at low tdr concentration incorporation induced by t4 chi is reduced and that induced by t4 omega is increased compared to wild type. no differences between wild type and mutant induced tdr ...	1983	6355762
[topology of the structural proteins of long tail fibers of phages t4d, ddvih+ and ddvih].	topology of the products of the genes 34, 35, 36 and 37 of the bacteriophage t4d long tail fibers were determined with the aid of monospecific antibodies. the antibodies against gene product 34 were the only to interact with the proximal part of long tail fibers, but the distal part bound the antibodies against 35, 36 and 37. product of the gene 35 is located at the joint-site with the distal part and binds the distance not more than 75 a long. gene product 36 is located between these of 35 and ...	1983	6355821
t4-labeling technique and its applications with particular reference to blood group antigens in bladder tumors.	a variety of visual markers have been developed to detect specific cell surface components with scanning electron microscopy. among them bacteriophage t4 (t4) has the most distinctive shape consisting of a hexagonal head and tail. this characteristic shape of t4 makes it possible to survey the cell surface at relatively low magnification and to analyze the site specificity at high magnification. although precise localization of surface molecules cannot be mapped due to the relatively large size ...	1983	6356335
influence of local nucleotide sequence on substitution of 2-aminopurine for adenine during deoxyribonucleic acid synthesis in vitro.	three highly purified dna polymerases, escherichia coli polymerase i (enzyme a) and the polymerases induced by wild-type t4 phage and by t4 phage mutant l141 (antimutator phenotype), have been examined with respect to their tendency to incorporate the deoxyribonucleotide of 2-aminopurine [(ap)] for deoxyadenylate at specific sites in deoxyribonucleic acid (dna). using phi x174 phage dna as a template and selected phi x174 restriction fragments as specific primers, we synthesized short sequences ...	1983	6357277
in vitro deletional mutagenesis for bacterial production of the 20,000-dalton form of human pituitary growth hormone.	the 20,000-dalton (20k) variant form of human growth hormone (hgh) present in extracts from pituitary glands differs from the major form of hgh (22k, 191 amino acids) by the deletion of amino acid residues 32-46. using oligonucleotide-mediated mutagenesis, the dna coding for these amino acids was deleted from the gene previously constructed by us (goeddel et al., 1979) for microbial hgh production. the dna to be deleted was looped out by the annealing of a synthetic oligodeoxyribonucleotide to t ...	1983	6357679
differential effects of testosterone, thyroxine, and cortisol on rat submandibular gland versus renal kallikrein.	the effects of 17 alpha-methyltestosterone (t alpha), t4, or cortisol (f) on tissue kallikrein in the rat submandibular gland and renal cortex were measured. castrated male or normal female sprague-dawley rats were treated with t alpha or t4 for 2 weeks. in addition, f, t alpha, or t4 was given to adrenalectomized female rats for 2 weeks. in the submandibular glands of male rats, castration resulted in a significant reduction of both kallikrein-like alpha-n-tosyl-l-arginine methyl ester (tos-arg ...	1983	6357767
hormonal profile and glucose tolerance in late pregnancy and postpartum.	oral glucose tolerance, plasma insulin and basal levels of glucagon, hgh, hprl, hpl, tsh, t4, t3, thyroxine-binding globulin (tbg), cortisol, corticosteroid-binding globulin (cbg) and estriol were measured in 23 normal pregnant women in late gestation (31 +/- 0.4 weeks of pregnancy). twelve of these subjects could be re-examined 14 +/- 2 weeks postpartum. blood glucose was lower basal and after glucose load (100 g) in the pregnant group. fasting plasma insulin and glucose-induced insulin release ...	1983	6357985
an epidemic of group a, type 4 streptococcal carriers among school children and their desk location at school.	during a follow-up study of pharyngeal carrier of beta-hemolytic streptococci among school children in three classes (ages 8-9) in sapporo city, an epidemic of group a, t4 streptococcal carrier was observed. the epidemic started in february 1978 in class ii (35 pupils) and spread to class i (36 pupils) in may. class iii developed only three carriers during the course. information on the desk location of those children at school and those on some host factors or on several environmental factors w ...	1983	6358701
physical and genetical analysis of bacteriophage t4 generalized transduction.	this report describes a comparison of the efficiency of transduction of genes in e. coli by the generalized transducing bacteriophages t4gt7 and p1cm. both phages are capable of transducing many genetic markers in e. coli although the frequency of transduction for particular genes varies over a wide range. the frequency of transduction for most genes depends on which transducing phage is used as well as on the donor and recipient bacterial strains. analysis of t4gt7 phage lysates by cesium chlor ...	1983	6358795
effect of propylthiouracil, and of bacterial endotoxin (lps), on thyroid hormones, respiratory rate, cutaneous and renal blood flow in rabbits.	rabbits in a warm environment reacted to i.v. injections of 10 mg/kg propylthiouracil (ptu) with an immediate fall of serum triiodothyronine (t3) concentration, associated with decreases of respiratory rate and cutaneous blood flow. simultaneously renal blood flow increased, while arterial blood pressure fell slightly. a rise in the animals' core temperature by 1.1 degree c, on average, contributed to the impression that ptu mimicked the stimulation of the normal thermoregulatory response patter ...	1983	6359054
polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of dna or deoxyribooligonucleotides by t4 dna ligase in polymer solutions.	the rates of blunt-end and cohesive-end ligation of dna by t4 dna ligase are increased by orders of magnitude in the presence of high concentrations of a variety of nonspecific polymers such as polyethylene glycol, ficoll, bovine plasma albumin, or glycogen. blunt-end ligation of small self-complementary oligodeoxyribonucleotides is also stimulated. the use of polyethylene glycol 6000 in such systems is characterized in some detail. conditions are suggested which either greatly increase the rate ...	1983	6359064
anticodon loop size and sequence requirements for recognition of formylmethionine trna by methionyl-trna synthetase.	previous work from our laboratory identified several specific sites in escherichia coli trnafmet that are essential for recognition of this trna by e. coli methionyl-trna synthetase (ec 6.1.1.10). particularly strong evidence indicated a role for the nucleotide base at the wobble position of the anticodon in the discrimination process. to further investigate the structural requirements for recognition in this region, we have synthesized a series of trnafmet derivatives containing single base cha ...	1983	6359155
identification of bacteriophage t4d gene 29 product, a baseplate hub component, as a folylpolyglutamate synthetase.	an assay for folylpolyglutamate synthetase activity in extracts of uninfected and bacteriophage t4d-infected escherichia coli b has been developed. t4d infection induced the formation of a new synthetase raising the total synthetase activity three-fold. extracts obtained after infection with t4 gene 51, 27 or 28 amber mutants showed increased synthetase activities while extracts obtained from cells infected with a t4d gene 29 amber mutant did not show any increase in synthetase activity. the pha ...	1983	6360172
the distribution of lymphoid and macrophage like cell subsets of sarcoid and kveim granulomata: possible mechanism of negative ppd reaction in sarcoidosis.	immunohistological observations of lymphoid and non-lymphoid cell subsets in biopsies of sarcoid skin granulomas have been compared with positive kveim tests and the sites of ppd injection in sarcoid patients. monoclonal antibodies have been used in indirect immunofluorescence often in combination with histochemical methods for the detailed characterization of the cells involved. the antibodies used included two new reagents, rfd-1 and rfd-2, which react with interdigitating cells and acid phosp ...	1983	6360446
thyroid hormones in milk: physiological approach--a review.	reported values of the concentration of thyroid hormones in milk vary substantially. there are some doubts on the specificity of methods used for their estimation. we aimed, therefore, to study the effects of mother milk on thyroid function parameters in sucklings as well as at the effect of milk secretion on maternal thyroid. according to fukuda et al. [1980] the lactation induces a hypothyroid state in the rat (high tsh, low thyroid hormones in maternal plasma). in our study the weaning of 18- ...	1983	6360658
effect of thyroid hormones from maternal milk on some measures of body growth and endocrine system in newborn sucklings.	the effect of thyroidectomy (tx) in lactating mother on the development of sucklings was studied during first 15 days of life. tx resulted in a decrease of thyroxine (t4) level in maternal milk which further resulted in a decrease of the level of t4, insulin and glucose in serum of newborns, and a decrease of body weight and of the glycogen content in liver. it is suggested that the hypoglycemia, decrease of liver glycogen and retardation of body growth under such conditions result from the inhi ...	1983	6360659
[effect of thyroid hormones on the beta-adrenergic receptor internalization in isolated rat hepatocytes].	the effect of the thyroid hormone(t4) on the internalization of beta-adrenergic receptors in isolated hepatocytes was examined. uptake of [3h]-dihydroalprenolol(dha) by the cells, which were prepared from untreated-rats, was increased by the time-dependent manner in vitro. the amount of incorporated-[3h]-dha was decreased by further incubation in the presence of 10(-5) m of d, 1-isoproterenol. however, about 30% of incorporated-[3h]-dha remained in the cells by this treatment. the amount of this ...	1983	6321250
triiodothyronamine--a beta-adrenergic metabolite of triiodothyronine?	triiodothyronamine (triam) is a potential metabolite of triidothyronine (t3), resulting from decarboxylation of the side-chain. in an attempt to elucidate the physiological properties of triam we have investigated the binding of triam to beta-adrenergic receptors, using turkey-erythrocytes and performing binding studies with ( (-)(3h)-dihydroalprenolol) ( (-)(3h)-dha) as a specific beta-adrenergic ligand. the inhibition constant ki for triam was determined as 5 x 10(-6) m, compared to dopamine ( ...	1983	6319258
immunological monitoring of viral infections in renal transplant recipients.	twelve viral episodes occurred in 54 antilymphocyte globulin-treated renal transplant recipients (two primary cytomegalovirus, seven cytomegalovirus reactivations, one chickenpox, two influenza). in 11 of 12 cases the ratio between peripheral t4 and t8 subsets fell (from 1.698 to 0.986, p less than 0.01) due both to a reduction of t4+ and to an increase of t8+ cells. t10+ and 5/9 subsets also increased. suppressor t-cell function, as measured by two simultaneous assays, was enhanced. in antibody ...	1983	6318220
contributions of various types of damage to inactivation of t4 bacteriophage by protons.	mean doses for damage induced by 3.7-mev protons in t4 phage were measured for the following effects: inactivation, killing, adsorption, dna injection, capsid rupture with dna release, and single- and double-strand dna breaks. these effects have been related to phage survival in the same experiment because of the variability inherent in such measurements. the experiments were carried out in nutrient broth, phosphate buffer, and phosphate buffer plus histidine as suspension media. the following c ...	1983	6318256
nuclear specific thyroxine binding in human mononuclear blood cells.	a method for measuring specific, high affinity, low capacity nuclear binding sites for thyroxine in human mononuclear blood cells is described. the binding is temperature and ph dependent and saturable. the cross-reactivity of triiodothyronine for the thyroxine binding sites was only 5% at the 50% inhibition level. chromatographic and electrophoretic studies suggested two nuclear thyroxine binding proteins with a molecular weight of 66,000 and 140,000 daltons. maximal specific nuclear binding ca ...	1983	6318301
isolated acth deficiency accompanied by 'primary hypothyroidism' and hyperprolactinaemia.	a 55 year old man with isolated acth deficiency is reported. the lesion would appear to be located in the pituitary gland since plasma acth and cortisol did not respond to lysine vasopressin and corticotrophin releasing factor (crf). a fall in t4, a rise in basal values of tsh, prolactin (prl), lh and fsh, excessive responses of tsh and prl to trh, and hyperreactive responses of lh and fsh to lrh were observed. these hormonal changes were examined before and after administration of cortisol. the ...	1983	6318488
nuclear triiodothyronine receptors and metabolic response in perinatally protein-deprived rats.	pregnant rats from day 14 of pregnancy and pups were fed a control diet (24% casein) or a deprived diet (8% casein) to obtain a control and a pre- and post-natal protein-deprived group. from 50 days of age, all groups were fed a balanced commercial stock diet for different periods. a significant reduction in body weight was observed in the perinatally protein-deprived group (ppd) after the different periods of nutritional recovery studied. maximal binding capacity (mbc) and apparent affinity con ...	1983	6318491
use of t4 dna polymerase replacement synthesis for specific labeling of plasmid-cloned inserts.	a strategy employing t4 dna polymerase replacement synthesis is described whereby only the insert portion of recombinant plasmids are radioisotopically labeled. prior purification of the inserted dna is not required. the recombinant plasmid is first digested with one or more restriction endonucleases selected to cleave the vector segment into fragments at least 30% shorter than the insert dna segment. this mixture of fragments is then digested by the t4 dna polymerase-associated 3' exonuclease i ...	1983	6318604
thyroid hormones and the reactivities of genetic variants of human erythrocytic glucose-6-phosphate dehydrogenase.	thyroid hormones, throxine (t4) and triiodothyronine (t3) which are known to activate glucose-6-phosphate dehydrogenase (g6pd) activity in vivo act as substrate inhibitors of g6pd in vitro. t4 competitively inhibits nadp in human erythrocyte g6pd variants g6pda, g6pdb and g6pda- with inhibition constants of 2.40 +/- 0.90 x 10(-6), 3.44 +/- 0.63 x 10(-6) and 6.53 +/- 0.60 x 10(-6) mol/l, respectively. the inhibition is, however, noncompetitive with respect to g6p in the three variants. t3 also ha ...	1983	6317373
modulation of thyroid hormone nuclear receptor levels by l-triiodothyronine (t3) in the rat pituitary.	the concentration of thyroid hormone nuclear receptors varies from one tissue to another, the anterior pituitary (ap) gland possessing the highest. since 3,5,3',1-triiodothyronine (t3) controls within a narrow range the secretion of tsh from the pituitary gland, this study was carried out to establish whether t3 modulates its own pituitary nuclear receptors and if so, whether this modulation is correlated with the thyroidal status and tsh secretion. salt-solubilized t3 nuclear receptors were mea ...	1983	6317485
corticosteroids in serum of rana catesbeiana during development and metamorphosis.	radioimmunoassays (ria) for aldosterone (aldo), corticosterone (b), and cortisol (f) were used to measure corticosteroids in serum of rana catesbeiana tadpoles and of young and adult frogs. because of the uncertain identity of the cortisol-like material in the ria, it was designated "f." serum aldo was detectable in tadpoles by stage vii and it remained at about 2 ng/ml in most stages until midclimax; then its concentration rose to high levels in froglets and frogs. serum b appeared later in dev ...	1983	6317513
gene 49 endonuclease vii is not essential for multiplicity reactivation of bacteriophage t4.	endonuclease vii, the product of phage t4 gene 49, has been shown previously to resolve holliday structures in vitro. two different processes, genetic recombination and multiplicity reactivation are presumed to have holliday structure intermediates. other workers have shown that genetic recombination is reduced in a gene 49 mutant infection. however, in the present study, multiplicity reactivation of uv-irradiated ts or amber mutant phage defective in gene 49 was nearly identical to that of uv-i ...	1983	6318042
transfusion-associated cytomegalovirus infection and acquired immune deficiency syndrome in an infant.	an infant who received multiple blood transfusions in the neonatal intensive care unit developed a transfusion-associated cmv infection at age 11 weeks and thereafter was noted to have hepatosplenomegaly, mitogen hyporesponsiveness, persistent viruria, an abnormal distribution of t-lymphocyte subpopulations, and poor growth. he has had recurrent opportunistic infections, including pneumocystis carinii pneumonia. six donors of blood products received by this infant were investigated; one was foun ...	1983	6315905
energetics of dna twisting. i. relation between twist and cyclization probability.	the twisting potential of dna has been determined directly by a method that measures the cyclization probability or j-factor of ecori restriction fragments as a function of dna twist. the cyclization probability is proportional to kc, the equilibrium constant for cyclization of the restriction fragment via its cohesive ends (shore et al., 1981). here we vary the twist of the dna by making small internal additions to or deletions from a 242 bp ecori restriction fragment. a series of 12 dna molecu ...	1983	6315955
adult t-cell lymphoma leukemia in western countries.	a new t-cell disorder has recently emerged: the so-called adult t-cell lymphoma leukemia (atll) initially described in japan. subsequently, atll cases were recognized in patients from the caribbean. we summarize the clinical and hematological features of 19 published cases from western countries, in addition to a new case we encountered. the leukemic cells display characteristic morphological features and a t3+t4+t8-t6- surface antigenic phenotype. overall survival is of short duration, but remi ...	1983	6316778
comparison of three methods for measuring locations of long range base pairing in single-stranded dna by electron microscopy.	a comparison was made of three procedures used for measuring the locations of long range base pairing in single-stranded dna. all three procedures gave equivalent results for the location of a transposon, tn903, and for a loop created by pairing between the nut and operator segments on an ecori restriction fragment of lambda dna. dna molecules prepared by a t4 gene 32 protein procedure had the best contrast. the highest frequency of measurable loops resulted from a procedure which utilized ammon ...	1983	6316805
a high affinity thyroid hormone binding protein in the cytosol of embryonic rat brain cells in primary cultures.	a thyroid hormone binding protein(s) has been characterized in the cytosol of fetal rat brain cells in primary cultures. this protein is closely related to the one described in brain supernatants with respect to its electrophoretic mobility, binding kinetic parameters and estimated molecular weight (65 000 daltons). however, in contrast to the brain cytosolic binding protein, two classes of affinity sites for triiodothyronine (t3) and thyroxine (t4) have been demonstrated: a high affinity site ( ...	1983	6316973
thyroid hormone receptors in human cultured fibroblasts: evidence for cellular t4 transport and nuclear t3 binding.	in cultured normal human skin fibroblasts specific and saturable binding sites for triiodothyronine (t3) have been revealed. in fact radiolabelled t3 binds rapidly to intact cells with maximum uptake after 1 hour, while nuclear binding is delayed, the equilibrium being reached after 2 hours. in intact cells it is possible to identify a single binding site for 125i-t3, with a ka = 1.8 x 10(10)m-1 and ro = 1.25 x 10(-11)m, similarly in nuclei it was possible to identify a single binding site of ka ...	1983	6303932
changes in t-lymphocyte subsets during childhood epstein-barr virus infectious mononucleosis.	lymphocyte subsets were measured using monoclonal antibodies in 11 children with epstein-barr virus-induced infectious mononucleosis and compared with those of 10 normal children. in acute infectious mononucleosis the percentage of t8+ lymphocytes was greater while the percentage of t4+ lymphocytes and the t4+ to t8+ ratio were less than those measured in normal children. the percentage and absolute number of t lymphocytes, as enumerated by e rosetting, did not differ from the values for normal ...	1983	6304139
pituitary receptors during development in the rat. i. trh binding capacity.	in order to study the role of thyroid releasing hormone (trh) in the control of thyroid stimulating hormone (tsh) secretion during the neonatal period, we measured the binding of [3h]-trh to pituitary homogenate of rats at various stages of development. in 2-day-old animals, the number of [3h]-trh binding sites were similar to that of adult male animals (23 +/- 8 fmole/mg protein versus 21 +/- 7 fmole/mg protein, respectively). between 5-21 days of age, the number of [3h]-trh binding sites was s ...	1983	6304605
limited proteolysis studies on the escherichia coli single-stranded dna binding protein. evidence for a functionally homologous domain in both the escherichia coli and t4 dna binding proteins.	limited proteolysis can be used to remove either 42 or 62 amino acids at the cooh terminus of the 18,873-dalton escherichia coli single-stranded dna binding protein (ssb). since poly(dt), but not d(pt)16, increases the rate of this reaction, it appears that cooperative ssb binding to single-stranded dna (ssdna) is associated with a conformational change that increases the exposure of the cooh terminus to proteolysis. as a result of this dna-induced conformational change, we presume that the cooh ...	1983	6298232
evidence for thyroid hormone receptors in uterine nuclei.	nuclear preparations from the rat uterus contain specific, high affinity binding sites for t3, with the properties expected of a thyroid hormone receptor. the kd value for the binding of t3 to these sites is 9.3 +/- 0.1 x 10(-10) m and the maximum number of binding sites is 0.11 +/- 0.02 pmoles t3 bound per mg of dna. competition experiments with triac, d-t3, t4, and rt3 compared to l-t3 revealed relative affinities of 87%, 22%, 15%, and 1.6% respectively. t3 binding sites solubilized from uteri ...	1983	6298566
chronic effect of tsh on human thyroid tissue in organ culture.	the chronic effect of tsh on thyroidal camp concentrations and release of thyroid hormones was investigated using human thyroid tissue in organ culture. normal human thyroid slices were placed in ham's f-10 synthetic culture medium in falcon organ tissue culture dishes, and incubated at 37 degrees in a humidified atmosphere of 5% co2 in air. medium was changed everyday and daily t3 or t4 release was determined using concentration of t3 or t4 in the medium. after incubation, slices were transferr ...	1983	6298795
effects of a polybrominated biphenyl mixture in the rat and mouse. i. six-month exposure.	a 1973 environmental accident in michigan resulted in exposure of humans via the food chain to polybrominated biphenyl (pbb). to better characterize the toxicity of the halogenated polycyclic aromatic hydrocarbon class of chemicals, rodents were dosed with pbb and their target organs examined for morphological, histological, biochemical, and selected endocrine changes. male and female rats and mice were given 125 po doses of pbb over a 6-month period at 0.1, 0.3, 1.0, 3.0, and 10.0 mg/kg of body ...	1983	6302948
structural features of very fast sedimenting dna formed by gene 49 defective t4.	very fast sedimenting dna (vfs dna) of t4 phage, which is formed by infection with a mutant in gene 49, was examined by electron microscopy after mild treatment with dnase i. it showed y-shaped, branched strands in addition to linear strands. each branch contained a single-stranded interruption about 60 nucleotides long at its proximal end. the average number of branches per t4 dna unit was close to the average number of sites susceptible to gene 49 nuclease. both numbers were consistently chang ...	1983	6302980
binding of thyroid hormones to isolated hepatic nuclei from rana catesbeiana tadpoles.	this study is concerned with the characteristics of thyroid hormone binding to isolated hepatic nuclei from premetamorphic tadpoles. conditions essential for nuclear stability and/or demonstration of saturable binding included 220-mosmol buffers containing 0.1 mm zncl2 and removal of most of the melanin granules; binding of t4 and t3 to melanin was significant, but unsaturable. scatchard analysis of [125i]t3 binding to nuclei in the presence of increasing concentrations of t3 revealed the presen ...	1983	6303753
tsh secretion and regulation in endemic goiter and endemic cretinism.	in endemic goiter patients living in a chronic iodide deficiency area (less than 50ug/day) tsh basal levels and the tsh response to trh is significantly higher (31 +/- 8uu/ml) than in normal controls (10 +/- 4uu/ml). there is a negative correlation between levels of iodine intake and serum tsh concentration and thyrotropin is thus increased when iodine decreased. also it was shown that serum tsh levels correlate inversely with the serum concentration of t4 (r=0.69) but not with the usually norma ...	1983	6304776