Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
tubulin post-translational modifications in the primitive protist trichomonas vaginalis.using several specific monoclonal antibodies, we investigated the occurrence and distribution of different post-translationally modified tubulin during interphase and division of the primitive flagellated protist trichomonas vaginalis. immunoblotting and immunofluorescence experiments revealed that interphasic microtubular structures of t. vaginalis contained acetylated and glutamylated but non-tyrosinated and non-glycylated [brugerolle and adoutte, 1988: bio systems 21: 255-268] tubulin. immuno ...19968801034
molecular data suggest an early acquisition of the mitochondrion endosymbiont.the three deepest branching eucaryotic lineages in small subunit ribosomal rna phylogenies are the amitochondriate microspora, metamonada and parabasala. they are followed by either the euglenozoa (e.g. euglena and trypanosoma) or the percolozoa as the first mitochondria-containing eucaryotes. to investigate the hypothesis of an even earlier timing of the mitochondrion endosymbiosis we have amplified a partial cpn-60 coding region from the parabasalid trichomonas vaginalis and the first such seq ...19968805838
genomic organization and sequence conservation in type i trichomonas vaginalis viruses.to reveal the genetic conservation of type i trichomonas vaginalis viruses (tvv) we cloned and sequenced the 4.6-kb ds rna of a tvv-t5 isolate for comparison with the cdna sequence of a related tvv-t1 ds rna. analogous to tvv-t1, the tvv-t5 ds rna also contains an upstream capsid protein gene overlapped with a downstream rna-dependent rna polymerase (rdrp) gene by a +1 reading frame shift. a conserved ribosomal slippage heptamer (c cuu uuu) was found within the consensus 14-nt overlap, and the c ...19968806533
the contribution of the arginine dihydrolase pathway to energy metabolism by trichomonas vaginalis.the enzymes of the arginine dihydrolase pathway were measured in trichomonas vaginalis hydrogenosome-deficient lines mr-5 and mr-100, and the parent strain tv 10-02. the activities and substrate affinities of arginine deiminase, carbamate kinase and ornithine decarboxylase were similar for the hydrogenosome-deficient lines and the parent tv 10-02. the activity of catabolic ornithine carbamyltransferase, however, was found to be 5-7-fold elevated in the hydrogenosome-deficient lines; the apparent ...19968813682
testing for hiv and other sexually transmitted diseases: implications for risk behavior in women.a sample of 808 nonpregnant women residing in an area of high prevalence of sexually transmitted diseases (stds) was studied with respect to sexual risk behaviors in the 4-month period before and after testing for a series of stds. all women were tested for both chlamydia trachomatis and trichomonas vaginalis and were given the option of also taking a test for the hiv antibody. neither the experience of receiving a negative hiv test result nor that of receiving a positive versus a negative diagn ...19968818671
[characterization of the partially purified proteinase from trichomonasvaginalis].characterization of a purified proteinase from trichomonas vaginalis was carried out using bacitracin-sepharose affinity chromatography. trichomonas vaginalis kt-9 isolate was used as a source of enzyme study. proteinase activity was determined using bz-pro-phe-arg-nan as the substrate. optimum ph for the purified proteinase activity was 7.0 and 6.0, 9.0 with dtt. optimum temperature was 37 degrees c and isoelectric point was 7.2. activity of this proteinase was inhibited by e-64, antipain, leup ...19968820741
molecular approaches to the diagnosis of male infertility.the introduction of the polymerase chain reaction (pcr) as a technique to selectively amplify and identify specific dna and rna sequences has revolutionized the field of molecular medicine. application of these newly developed molecular techniques to the field of male infertility has made the delineation of subtle causes of infertility-an inapparent genital tract infection, immune system activation within the genital tract, mutations in sperm mitochondrial or chromosomal dna, alterations in sper ...19969238679
reported condom use is not associated with incidence of sexually transmitted diseases in malawi.to establish frequency of reported condom use and validate reliability of self-reporting among urban women in malawi.19968838710
the polyurethane female condom: increasing the choice for women.the polyurethane female condom is manufactured exclusively in london by chartex international and marketed under the names reality, femidom, and femy. the device consists of a disposable, odorless polyurethane sheath 17 cm long. there is a loose inner ring at the closed end of minimum diameter 5.0 cm, and a softer, fixed ring at the open, outer end of minimum diameter 6.5 cm. each condom is prelubricated with dimethicone, a non-spermicidal lubricant. the female condom was designed to be used ...199612291292
medical microbiologythis chapter discusses human protozoan parasites belonging to seven different genera—- giardia, trichomonas, dientamoeba, chilomastix, balantidium, isospora, and cryptosporidium. giardia, trichomonas, chilomastix and dientamoeba are classified as flagellates; balantidium coli is a ciliate, and isospora and cryptosporidium are coccidians. all are intestinal parasites that are transmitte ...199621413275
medical microbiologygenitourinary infections fall into two main categories: (1) primary infections due to sexually transmitted pathogenic microorganisms and (2) infections due to members of the resident flora. genital infections are uncommon in children and increase dramatically in sexually active adults, in whom sexually transmitted diseases are the second most prevalent group of reportable communicable illness in north america. sexual ...199621413302
kinetics of killing or growth of trichomonas vaginalis in the presence of aminoglycosides, neomycin and geneticin (g418).few therapeutic drugs, other than metronidazole and close derivatives, are currently available to treat trichomoniasis. trichomonas vaginalis is known to have a primitive ribosome based on small subunit ribosomal dna sequence comparisons. targeting ribosomal subunits, the aminoglycosides neomycin and geneticin (g418, distinct from gentamycin) were tested for activity against t. vaginalis. while neomycin had no effect, g418 was effective in killing cultured t. vaginalis cells at doses intermediat ...199618611765
new concepts in the diagnosis and pathogenesis of trichomonas vaginalis.trichomonas vaginalis infection is the most commonly encountered sexually transmitted disease. there is a need for more accurate and rapid laboratory diagnostic methods, leading to better control and treatment strategies. various virulence factors such as adherence, contact-independent factors, hemolysis and acquisition of host macromolecules have been shown to play a role in the pathogenesis of this infection. detection of the factors that are only present in the pathogenic isolates of trichomo ...199622514457
trichomonas vaginalis weakens human amniochorion in an in vitro model of premature membrane rupture.trichomonas vaginalis (tv) infection is associated with preterm rupture of membranes (prom) and preterm birth. we evaluated the effects of tv growth and metabolism on preparations of human amniochorion to understand and characterize how tv may impair fetal-membrane integrity and predispose to prom and preterm birth.199518475407
hiv and stds: how are they linked? [letter].i write in response to dr. gina dallabeta's article with this title (hiv and stds: how are they linked?). prospective studies in sub-saharan africa have established the following: 1. seropositive commercial sex workers have a higher prevalence of genital ulcer disease (gud) and positive serology for syphilis. 2. seropositive men with chancroid more frequently have a history of prior gud. 3. seronegative men with a history of recent contact with a commercial sex worker are more likely to seroconv ...199512288717
identification of a satellite double-stranded rna in the parasitic protozoan trichomonas vaginalis infected with t. vaginalis virus t1.co-infection by a 0.5-kb small double-stranded (ds) rna together with trichomonas vaginalis virus (tvv) genomic 4.6-kb dsrna is commonly observed in a number of t. vaginalis isolates. by molecular cloning and primer extension experiments, the 497-bp cdna sequence of a 0.5-kb dsrna co-infecting with tvv-t1 in t vagina/is t1 isolate was elucidated. consistent with the replication cycle of a typical dsrna virus, a plus-strand viral rna beginning at +1 of the 0.5-kb dsrna was identified in infected ...199511831699
use of an animal model of trichomoniasis as a basis for understanding this disease in women.studies of host-parasite relationships in bovine trichomoniasis are presented as a basis for understanding mechanisms of pathogenesis and protection in trichomoniasis in women. the bovine infection is caused by trichomonas foetus, a trichomonad species closely related to the human pathogen trichomonas vaginalis. an estrogenized murine model infected with t. vaginalis is useful for the study of virulence factors, but the bovine model has advantages for the study of immunity because t. foetus infe ...19958845444
prevalence of vaginal infections with bacterial vaginosis, trichomonas vaginalis and candida albicans among pregnant women at the port moresby general hospital antenatal clinic.a clinico-sociodemographic and microbiological survey was carried out at the port moresby general hospital antenatal clinic to determine the prevalences of bacterial vaginosis, trichomonas vaginalis and candida albicans vaginal infections in pregnancy and to examine if the infections had any association with some suspected sociodemographic risk factors. the study was carried out between december 1990 and january 1991. of 206 consecutive subjects surveyed, 79 (38%) had symptomatic infection. howe ...19959522855
[the action of an aqueous extract of aloe barbadensis miller in an in-vitro culture of trichomonas vaginalis].the antiparasitic action of an aqueous extract of aloe barbadensis miller against and in vitro culture of trichomonas vaginalis was studied for the first time. three strains of this parasite were used for the study. taking an initial concentration of 400 mg/ml of the extract, double serial dilutions were performed, and final concentrations based on the dried weight of the extract were 10.4, 20.8, 41, 83, and 160 mg/ml. within 24 hours, percentages of inhibition greater than 50% were obtained fro ...19959813472
in-vitro activity of purpuromycin and mdl 63,604 against microorganisms that cause vaginitis and vaginosis.purpuromycin and its semi-synthetic derivative mdl 63,604 had in-vitro activity similar to that of amphotericin b against isolates of candida albicans. mdl 63,604 had activity similar to that of metronidazole against trichomonas vaginalis. both compounds were very active against most species of gram-positive and gram-negative anaerobes and against gardnerella vaginalis. mdl 63,604 had significantly lower mics than purpuromycin against t. vaginalis and most of the bacteria, probably due to antago ...19958821607
papillomavirus in cervicovaginal smears of women infected with human immunodeficiency virus.it has been described that women infected with the human immunodeficiency virus (hiv) present more frequent cytological abnormalities in cervicovaginal smears, generally related to infection by human papillomavirus (hpv). the present work is a study of cervicovaginal smears of 147 hiv-seropositive women submitted to routine gynecological examinations. the smears were stained by the papanicolaou method. cytopathic effects of hpv were found in 38 (25.8%) cases. nuclear atypias of cervical intraepi ...19958731285
cytotoxicity of hamycin for trichomonas vaginalis, hela and bhk-21.hamycin, a polyene antibiotic related to amphotericin b, has been used topically to treat fungal and protozoan infections in india. we assessed the cytotoxic activity of hamycin on nine metronidazole resistant or susceptible trichomonas vaginalis strains isolated from symptomatic or asymptomatic patients. cytotoxic activity of hamycin against two mammalian cell lines, bhk-21 and hela, was also determined. tritiated thymidine pulse labeling after drug-washout and a recovery period was used to dis ...19958626260
an improved method for in vitro susceptibility testing of trichomonas vaginalis.a protocol is presented for in vitro susceptibility testing of trichomonas vaginalis. a 100 ml culture of the microorganisms is prepared for inoculation into antibiotic dilutions and controls by centrifugation, washing with 10 mm hepes (ph 6.2) plus 1.5 x 10(-1) m nacl, a second centrifugation and a resuspension in the hepes-saline buffer. inclusion of the gelling agent carrageenan in the culture medium permits an ease of harvesting the trichomonads and a reproducible initial cell density of 1-4 ...19958718615
cytopathology and release of an rna virus from a strain of trichomonas vaginalis.a strain of trichomonas vaginalis infected with a double-stranded rna virus showed pronounced cytopathology in the form of giant syncytia generated by the recruitment of single cells. the giant cells ultimately lysed, releasing virus into the culture medium. in the infected cells, clusters of electron-dense particles resembling viral structures were found in the cytoplasm. in addition, distinctive inclusions composed of similar particles were present in the nuclei of some cells. double-stranded ...19958719959
serum antibodies to trichomonas vaginalis in invasive cervical cancer patients.to evaluate, by seroepidemiology, the possible role of the sexually-transmitted flagellate, trichomonas vaginalis, in invasive cervical cancer.19958566984
prevalence of trichomonas vaginalis in a male sexually transmitted disease clinic population by interview, wet mount microscopy, and the inpouch tv test.to determine the prevalence of trichomoniasis in male patients from their urine at a sexually transmitted disease (std) clinic using the inpouch tv culture system.19958566985
epidemiologic differences between candidial and trichomonal infections as detected in cytologic smears in taiwan.the epidemiologic differences between cytology-detected candidial and trichomonal infections were assessed in 15,933 women attending the 12 district health centres in the taipei area and a consecutive 1114 patients, visiting venereal disease clinics, whose smears were screened for cervical cancer between july 1991 and december 1992 in taipei. the pap smears were examined for the presence of specific organisms, such as trichomonas vaginalis, vaginal candida, herpes simplex virus, human papillomav ...19958570805
iron mediates trichomonas vaginalis resistance to complement lysis.trichomonas vaginalis, a sexually transmitted disease agent in humans, is readily lysed by activation of the alternative complement pathway. the parasite became resistant following growth in medium supplemented by iron compared to parasites grown in medium depleted of iron, which were readily killed by complement. the resistance to complement was dependent on iron concentration while divalent cations other than iron were ineffective, showing specific regulation of this property by iron. lactofer ...19958577239
the relationship of vaginal trichomoniasis and pelvic inflammatory disease among women colonized with chlamydia trachomatis.trichomonas vaginalis is a common sexually transmitted pathogen that has been linked to upper genital tract bacterial disease. its association with upper tract chlamydial disease has not been assessed.19958578405
[survival ability of trichomonas vaginalis in mineral baths].the role of mineral thermal baths and particularly of bath fittings such as benches and toilet seats in the spreading of trichomonas vaginalis is controversial. in order to clarify the possibility of transmission by smear infection of this ubiquitous urogenital parasite the ability to survive was investigated in 11 mineral baths in baden-württemberg by contamination of water samples of varying mineral content. in addition, trichomonas-contaminated water samples were placed in petri-dishes on dif ...19958580611
two trichomonas vaginalis surface proteinases bind to host epithelial cells and are related to levels of cytoadherence and cytotoxicity.recent reports strongly suggest that cytoadherence and cytotoxicity by trichomonas vaginalis require cysteine proteinase activity. because of the large number of cysteine proteinases synthesized by t. vaginalis, a ligand assay was used to identify specific proteinases which may selectively target host cells. two cysteine proteinases from trichomonal extracts with relative molecular masses (mr) of 65,000 daltons (65-kda) and 30-kda were found to avidly bind to hela cell and vaginal epithelial cel ...19958580681
root of the universal tree of life based on ancient aminoacyl-trna synthetase gene duplications.universal trees based on sequences of single gene homologs cannot be rooted. iwabe et al. [iwabe, n., kuma, k.-i., hasegawa, m., osawa, s. & miyata, t. (1989) proc. natl. acad. sci. usa 86, 9355-9359] circumvented this problem by using ancient gene duplications that predated the last common ancestor of all living things. their separate, reciprocally rooted gene trees for elongation factors and atpase subunits showed bacteria (eubacteria) as branching first from the universal tree with archaea (a ...19957708661
routine detection of trichomonas vaginalis in genital specimens using culture in microtitre trays.the culture of genital specimens for the detection of trichomonas vaginalis is generally regarded as the most sensitive technique available for routine use. however, the technique is relatively expensive and time-consuming, involving the preparation of one or more wet mounts from the culture to observe motile trichomonads. considerable savings in cost and time can be achieved by using culture in microtitre trays and visualisation with an inverted microscope.19958520256
trichomonas vaginalis and cervical cancer. a prospective study in china.the relationship between trichomonas vaginalis infection and cervical cancer was investigated prospectively in a cohort of 16,797 women aged 25 years or more who were followed from 1974 to 1985 within the framework of a cervical screening program in jingan, china. personal interviews were conducted by trained interviewers when the women first entered the screening program. at initial screening, 421 (2.51%) women had a positive cytologic diagnosis of t. vaginalis infection. ninety-nine incident c ...19958520717
examination of nonflagellate and flagellate round forms of trichomonas vaginalis by transmission electron microscopy.plasmodium-like round cells without sections of flagella, round flagellar cells with dividing nucleus and binuclear flagellate round forms were represented from the log-phase of trichomonas vaginalis cultures. the fine-structural characteristics of the round cells support the opinion that the cells without flagella as well as the round multinuclear flagellate cells represent developmental stages preceding the appearance of mononuclear flagellate cells. in the round cells, amitotic division was o ...19958528306
endocervical pathogens in women with preterm and term labour.the purpose of this study was to compare the endocervical microflora of women in preterm and term labour and to determine whether the presence of a specific microflora is significantly associated with preterm labor. a prospective study was performed in lithuania among 212 women in preterm labour (latent phase, n = 110; active phase, n = 102) and among 62 healthy women in term labour. microbiological assessment included cultures for aerobic bacteria, yeasts, and trichomonas vaginalis and direct i ...19958529951
free radical detoxification in giardia duodenalis.non-denaturing polyacrylamide gels were used to analyse superoxide dismutase (sod), catalase, peroxidase, nadh oxidase and nadh peroxidase in the microaerophilic protozoan parasite giardia duodenalis. a cytosolic h2o-producing nadh oxidase and membrane-associated nadh peroxidase were readily detected from g. duodenalis. in all giardia strains investigated the nadh oxidase was present in high levels (1.2-2 u (mg protein)-1). using the same technique, nadh oxidase activity was also detected in the ...19958538699
the relationship of experimental pathogenicity in vivo with in vitro cytoadherence and cytotoxicity of 6 different isolates of trichomonas vaginalis.the experimental pathogenic effects in vivo and in vitro of 6 different isolates of trichomonas vaginalis were studied following their inoculation into nmri mice and on to adherent cultures of hela cells. contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. the strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate wit ...19958550301
common genitourinary infections.vulvovaginal pain, itching, and burning are a triad of symptoms for which women frequently seek health care. often accompanied by vaginal discharge and dysuria, these symptoms account for as many as 5 million office visits a year. proper assessment and management of these symptoms by nurses, nurse practitioners, and nurse midwives can help to substantially improve a woman's quality of life and help prevent long-term problems. several differing syndromes or infections can be the cause of these sy ...19958551372
characterization of cdnas encoding adhesin proteins involved in trichomonas vaginalis cytoadherence.trichomonas vaginalis cytoadherence is mediated by four adhesins (ap65, ap51, ap33 and ap23). adhesin gene expression was previously shown to be up-regulated by iron. therefore, a cdna library was constructed from mrna of t. vaginalis grown in a high-iron medium. ten cdna clones (three for ap65, one for ap51, and six for ap33) were recognized with polyclonal antiserum or monoclonal antibody raised against these adhesins. no cross-hybridization among cdnas of the three adhesins was observed in so ...19958555730
sexually transmitted diseases in children and evidence of sexual abuse.during the period june 1989 to march 1991, laboratory evidence of sexually transmitted diseases (stds) was found in 107 patients at the red cross war memorial children's hospital in cape town. data was available on 96 patients aged 23 months to 14 years (mean 75.9 months). vaginal discharge was the most frequent presenting symptom (76%), particularly in those less than 5 years of age (90%). although a history of abuse was not given on presentation in 62%, evidence of abuse was subsequently elici ...19958556444
aetiology of vaginal infections in pregnant and non-pregnant women in barbados.the aetiology of vaginal discharge was studied in 175 barbadian women. pregnant women accounted for 52% (91) of the population studied. candida albicans was detected in 45% of the women, bacterial vaginosis in 28% and trichomonas vaginalis in 8.6%. bacterial vaginosis was more common in non-pregnant women (33% vs 23%) whereas c. albicans was more common in pregnant women (49% vs 39). t. vaginalis was found with almost equal frequency in pregnant and non-pregnant women. mixed infections were foun ...19958560888
clinical and epidemiological correlations between the infection with hpv 16 and hpv 18 and female cervical lesions.a number of 66 specimens from female cervical lesions were examined for infection with human papillomavirus (hpv) types 6, 11, 16, and 18 by nucleic acid hybridization in dot-blot techniques and 35 sera were tested by the immunodot-blot technique, in order to detect the presence of anti e4 and e7 hpv protein antibodies. the findings were compared with the histologic diagnosis. fifty-six per cent of specimens contained hpv dna sequences. in 47% of specimens from cervical carcinoma, hpv 11 was det ...19959179967
genital tract infections in sexually active women in barbados.ninety-eight women attending three different clinics were prospectively studied for the presence of genital tract infections, including chlamydia trachomatis. of these 98 women, 35 were presenting to a polyclinic with symptoms of genital tract infection, 55 were attending an antenatal clinic for their first visit, and 8 referred to a colposcopy clinic because of an abnormal papanicolaou smear were included. gonorrhoea was detected in one patient, syphilis in two, and trichomonas vaginalis in six ...19958838049
[effect of sodium nitrite on trichomonas vaginalis].we have investigated the action of sodium nitrite on the growth and morphologic changes of t. vaginalis and on the treatment of subcutaneous abscess by trichomonad in mice. sodium nitrite inhibited the growth of metronidazole-sensitive kt9 isolate and metronidazole-resistant cdc85 strain of t. vaginalis as concentration of 6 mm and 10 mm, respectively. intraperitoneal injection of sodium nitrite (70 micrograms, 100 micrograms, 130 micrograms/g body weight) did not reduce the size of abscess prod ...19958591013
sexually transmitted diseases and human immunodeficiency virus infection among women with genital infections in burkina faso.this study reports the prevalence of sexually transmitted diseases (stds) among gynaecological outpatients presenting at the bobo-dioulasso hospital (burkina faso) with genital infections and examines the factors associated with hiv infection in this population. of 245 eligible non-pregnant women, 220 consented to participate in the study. seventy-seven per cent had sexually transmitted infections. the most common were: trichomonas vaginalis (28%), chlamydia trachomatis (27%), bacterial vaginosi ...19957548291
drug induced myopia associated with treatment for gynecological problems.we present three cases of women who developed acute transient myopia caused by drugs for gynecological problems. one patient was treated with disothiazide for premenstrual edema. the second had acute cystitis and was treated by sulphonamide and the third developed myopia coincident with metronidazole treatment for trichomonas vaginalis. we followed these patients by a scan ultrasonographic ocular measurements, documenting reduction of the anterior chamber depth combined with lens thickening. the ...19957549443
[effect of cladonia alpestris on trichomonas vaginalis in vitro].in this paper, an experimental research is reported on the effect of water extract of cladonia alpestris and s-(-) usnic acid on trichomonas vaginalis in vitro. the results showed that both the water extract of cladonia alpestris and s-(-)usnic acid exhibited a strong effect against trichomonas vaginalis in vitro. as the time of action of the agents was prolonged, the mortality of trichomonas vaginalis increased. for s-(-) usnic acid, 0.4 mg/ml was the lowest effective concentration against tric ...19957554162
[exploration on experimental conditions of dot-elisa for trichomonas vaginalis using intact parasite antigen-monoclonal antibody detecting system].this paper deals with the effect of different experimental conditions including the number of parasite, blocking fluid, incubation time and different types of antigens on dot-elisa for trichomonas vaginalis. five microlitres of freshly washed intact worms at a concentration of 7.7 x 10(5)-3 x 10(8) cells/ml were added onto nitrocellulose membrane blocked with 1% bsa, 10% bovine serum or milk power (0.1%, 0.25%, 0.5%, 5%). dot-elisa using nitrocellulose membrane containing whole cell antigen 2.5 ...19957554165
generation of interleukin-8 from human monocytes in response to trichomonas vaginalis stimulation.neutrophils are the predominant inflammatory cells found in the vaginal discharges of patients with trichomonas vaginalis infection. we have investigated the possible role of interleukin-8 (il-8) in the inflammatory response elicited by t. vaginalis infection. this study has shown that t. vaginalis induces blood monocytes to produce large amounts of bioactive il-8, mainly by membrane components of t. vaginalis (mtv). monocyte-derived il-8 induced by mtv was dose and time dependent. the peak leve ...19957558293
primary structure and eubacterial relationships of the pyruvate:ferredoxin oxidoreductase of the amitochondriate eukaryote trichomonas vaginalis.in the eukaryotic unicellular organism trichomonas vaginalis a key step of energy metabolism, the oxidative decarboxylation of pyruvate with the formation of acetyl-coa, is catalyzed by the iron-sulfur protein pyruvate:ferredoxin oxidoreductase (pfo) and not by the almost-ubiquitous pyruvate dehydrogenase multienzyme complex. this enzyme is localized in the hydrogenosome, an organelle bounded by a double membrane. pfo and its closely related homolog, pyruvate:flavodoxin oxidoreductase, are enzym ...19957563125
molecular and phylogenetic analysis of pcr-amplified cyclin-dependent kinase (cdk) family sequences from representatives of the earliest available lineages of eukaryotes.cyclin-dependent kinase (cdk) and cell division control (cdc2) sequences are strongly conserved among eukaryotes and may complement the use of other sequence families in eukaryotic phylogenetic inference. we synthesized degenerate pcr primers to amplify the catalytic region of cdk homologs in representatives of the earliest available lineages of eukaryotes. cdk family sequence-based, maximum-likelihood distance measurements with neighbor-joining, and fitch-margoliash least-squares analyses produ ...19957563127
paromomycin for nitroimidazole-resistant trichomonosis. 19957564825
carbohydrate involvement in the association of a prokaryotic cell with trichomonas vaginalis and tritrichomonas foetus.previous studies have shown that trichomonas vaginalis are capable of ingesting bacteria. this observation was confirmed in the present study and extended to tritrichomonas foetus. using a special strain of escherichia coli grown under conditions that produced fimbriae presenting a lectin-like molecule recognizing mannose, we showed that the bacteria attached to and were ingested by trichomonads through a mechanism involving cell-to-cell recognition. absence of the fimbriae or addition of alpha- ...19957567902
[bacteriological findings in patients with cervical intra-epithelial neoplasia].in order to evaluate the cervical flora in patients with histologically confirmed cervical intraepithelial neoplasia (grades i to iii) the microbiologic results of 216 patients with cin were compared with those of 100 symptom free controls. gardnerella vaginalis combined with mykoplasma spp. and bacteroides spp. were found in 22% of the patients with dysplasia and in 5% of the control group. the difference was significant (p < 0.004). microorganisms causing sexually transmitted diseases like nei ...19957571906
primary structure of the hydrogenosomal malic enzyme of trichomonas vaginalis and its relationship to homologous enzymes.the complete nucleotide sequence has been established for two genes (maea and maeb) coding for different subunits of the hydrogenosomal malic enzyme [malate dehydrogenase (decarboxylating) ec 1.1.1.39] of trichomonas vaginalis. two further genes (maec and maed) of this enzyme have been partially sequenced. the complete open reading frames code for polypeptides of 567 amino acids in length. these two open reading frames are similar with less than 12 percent pairwise nucleotide differences and les ...19957581334
trichomonas vaginalis: ultrastructural bases of the cytopathic effect.the in vitro cytopathic effect of trichomonas vaginalis on epithelial cells was explored through the interaction of trophozoites of the virulent strain gt-10 with mdck monolayers. the interaction was analyzed through electrophysiology, video microscopy, and transmission and scanning electron microscopy. electrical measurements revealed that living parasites produced severe damage to the cell monolayers within 30 min, manifested as a rapid decrease in transepithelial resistance. microscopic obser ...19957581342
uptake of amino acids by the parasitic, flagellated protist trichomonas vaginalis.hplc techniques have been applied to study amino acid uptake and release by trichomonas vaginalis under a variety of conditions. studies on the growth of t. vaginalis in complex media and the survival of the parasite in simple media, with and without amino acids and/or maltose, have shown that the growth or survival of t. vaginalis is better in the presence of maltose than when it is absent, and that greater amounts of amino acids are consumed by t. vaginalis in the absence of maltose. the resul ...19957582024
should screening of genital infections be part of antenatal care in areas of high hiv prevalence? a prospective cohort study from kigali, rwanda, 1992-1993. the pregnancy and hiv (ege) group.to study the prevalence and incidence of genital infections and their association with hiv-1 infection among pregnant women in kigali, rwanda.19957590709
ignored trichomonal infestation diagnosed by papanicolaou smear.to compare the occurrence of trichomonas vaginalis as demonstrated by culture and by papanicolaou (pap) smears in a sexually transmitted disease (std) clinic.19957590721
genital ulcers associated with human immunodeficiency virus-related immunosuppression in female sex workers in abidjan, ivory coast.a cross-sectional study among female sex workers in abidjan was conducted to study the association between sexually transmitted diseases and human immunodeficiency virus (hiv) infection and hiv-related immunosuppression. among 1209 women tested for hiv, 962 (80%) were seropositive. hiv infection was independently associated with a longer duration of sex work, a lower price for intercourse, being an immigrant, and having a positive treponema pallidum hemagglutination test (p < .05). genital ulcer ...19957594681
small extrachromosomal nucleic acid segments in protozoan parasites.viruses have been described in the following protozoa: babesia spp., trichomonas vaginalis, giardia lamblia, leishmania braziliensis and eimeria spp. in order to study the babesia bovis virus, merozoites have been prepared from the blood of infected cattle. agarose gel electrophoresis of nucleic extracts from the bovine protozoa b. bovis and babesia bigemina were separated into genomic dna and at least two additional nucleic acids. one molecule with a relative mobility of 5.5 kilobase pairs (kbp ...19957597793
pcr-based study of conserved and variable dna sequences of tritrichomonas foetus isolates from saskatchewan, canada.the protozoan parasite tritrichomonas foetus causes infertility and spontaneous abortion in cattle. in saskatchewan, canada, the culture prevalence of trichomonads was 65 of 1,048 (6%) among 1,048 bulls tested within a 1-year period ending in april 1994. saskatchewan was previously thought to be free of the parasite. to confirm the culture results, possible t. foetus dna presence was determined by the pcr. all of the 16 culture-positive isolates tested were pcr positive by a single-band test, bu ...19957615746
phylogeny of trichomonads inferred from small-subunit rrna sequences.small subunit (16s-like) ribosomal rna sequences were obtained from representatives of all four families constituting the order trichomonadida. comparative sequence analysis revealed that the trichomonadida are a monophyletic lineage and a deep branch of the eukaryotic tree. relative to the early divergent eukaryotic assemblages the branching pattern within the trichomonadida is very shallow. this pattern suggests the trichomonadida radiated recently, perhaps in conjunction with their animal hos ...19957620466
trichomoniasis in men: old issues and new data.trichomoniasis is common among sexually active women, but its importance as a cause of morbidity among sexually active men remains uncertain.19957624817
multidrug resistance and p-glycoproteins in parasitic protozoa.drug resistance has emerged as a devasting impediment to the treatment and control of diseases of parasitic origin. the underlying mechanisms that contribute to this drug resistance in field isolates, however, are poorly understood. members of the p-glycoprotein gene (pgp) family have been identified, cloned, and sequenced in plasmodia, leishmania, and entamoeba, and variations in pgp copy number and/or expression have been implicated as a basis for drug resistance in each of these genera. the s ...19957629055
lower genital tract infections in infertile nigerian women compared with controls.to investigate the possibility that infertile nigerian women have a higher rate of cervical colonisation with pathogenic and facultative organisms than fertile controls.19957635492
trichomonas vaginalis transmission in a family. 19957635505
barrier methods of contraception.although a recent cavalieri d'oro et al. article correctly concludes that while barrier methods reduce the risk of gonorrhea and hiv, they may be less consistent for other diseases, the review does not include the female condom, the newest method of barrier contraception. laboratory tests have shown polyurethane, the material of which the new condom is manufactured, to be impermeable to hiv and cytomegalovirus. similar permeability studies using bacteriophages smaller than hepatitis and hiv sh ...19957635509
analysis of human immunoglobulin-degrading cysteine proteinases of trichomonas vaginalis.trichomonas vaginalis is a protozoan parasite that causes a widely distributed sexually transmitted disease (std). since immunoglobulin g (igg) antibodies to specific trichomonad immunogens are found in serum and vaginal washes (vws) from patients with trichomoniasis, a potential mechanism of immune evasion by this parasite might be the ability of t. vaginalis proteinases to degrade human immunoglobulins (igs). incubation of human igg with lysates of t. vaginalis organisms resulted in time- and ...19957642267
amniotic fluid interleukin-6: correlation with upper genital tract microbial colonization and gestational age in women delivered after spontaneous labor versus indicated delivery.our purpose was to determine whether amniotic fluid interleukin-6 is increased and inversely proportional to gestational age in women with chorioamnion colonization and spontaneous labor versus women delivered for medical or obstetric indications.19957645642
cell cytotoxicity of sodium nitrite, sodium nitroprusside and roussin's black salt against trichomonas vaginalis.we have investigated the action of sodium nitrite and other nitrosyl complexes, such as sodium nitroprusside and roussin's black salt, on the growth of metronidazole-sensitive and resistant strains of trichomonas vaginalis and their hydrogenosomal enzymes. all three chemicals inhibited the growth of t. vaginalis: sodium nitrite at 8 mm, sodium nitroprusside at 1.2 mm and roussin's black salt at 0.2 mm. metronidazole-sensitive (kt9) and resistant (cdc85) isolates showed similar cytotoxicity again ...19957649439
the inflammatory papanicolaou smear: what does it mean?to determine the correlation between inflammation detected on papanicolaou smear and specific lower genital tract agents, and, based on these findings, to develop recommendations for follow-up tests and treatment of young women with inflammation on smears.19957651643
the pathway of secretion of proteinases in trichomonas vaginalis.trichomonads secrete large amounts of hydrolytic enzymes into liquid growth medium. proteinase release by trichomonas vaginalis has been quantified after resuspension of the parasite in a simple buffered maltose medium. after 6 h incubation, 70-90% of each of two cysteine proteinase activities, one towards benzyloxycarbonyl-arginyl-arginine 4-nitroanilide (z-rr-nan) and the other active towards n-benzoyl-prolyl-phenylalanyl-arginine 4-nitroanilide (bz-pfr-nan), was extracellular. this release wa ...19957657450
characterization of double-stranded rna satellites associated with the trichomonas vaginalis virus.three small and distinct satellite double-stranded rnas (dsrnas) denoted s1, s1', and s2 were recently described for a trichomonas vaginalis isolate harboring a dsrna virus. since characterization of these satellite dsrnas might provide insight into the virus replication cycle and virus-host interactions, full-length cdnas to s1 and s1' dsrnas were synthesized and sequenced. s1 dsrna has 688 bp, and s1' dsrna has 616 bp. a 228-bp open reading frame that begins at nucleotide 37 was detected on a ...19957474105
cloning and molecular characterization of two genes encoding adhesion proteins involved in trichomonas vaginalis cytoadherence.cytoadherence to the vaginal epithelium is a critical step in infection by the eukaryotic flagellate trichomonas vaginalis. four trichomonad surface proteins (ap65, ap51, ap33 and ap23) mediate cytoadherence. the cdna encoding the ap65 adhesin was isolated from a phagemid cdna expression library by screening with antiserum and monoclonal antibody (mab) raised against the purified trichomonad ap65 protein. two clones, f11.2 and f11.5, coded for immuno-crossreactive recombinant proteins that posse ...19957476210
analysis for adhesins and specific cytoadhesion of trichomonas vaginalis. 19957476404
screening for chlamydia trachomatis infection in pregnant women in martinique.to determine the prevalence of chlamydia trachomatis urogenital infection and to identify behavioral, demographic, and clinical factors associated with the infection in pregnant women in martinique.19957482104
spectrum of genital human papillomavirus infection in a female adolescent population.human papillomavirus infection is a sexually transmitted disease associated with cervical dysplasia and carcinoma.19957482107
male urethritis with and without discharge: a clinical and microbiological study.the definition of male urethritis in the absence of urethral discharge has not been well established. the sensitivity of urethral swabs and first-catch urine is controversial.19957482108
the preterm prediction study: significance of vaginal infections. national institute of child health and human development maternal-fetal medicine units network.our purpose was to evaluate the association of bacterial vaginosis, trichomonas vaginitis, and monilial vaginitis with spontaneous preterm birth at < 35 weeks 0 days.19957485327
pharmacodynamics of metronidazole determined by a time-kill assay for trichomonas vaginalis.the pharmacodynamic effects of metronidazole on trichomonas vaginalis have been poorly characterized. the present in vitro study was performed to characterize the relationship between killing of trichomonads and metronidazole exposure (metronidazole concentration and time of exposure). five laboratory strains and five recent clinical isolates of t. vaginalis were studied. the minimum lethal concentrations (mlcs) of metronidazole for the strains ranged from 0.8 to 25 micrograms/ml under anaerobic ...19957486930
concerted evolution in protists: recent homogenization of a polyubiquitin gene in trichomonas vaginalis.ubiquitin is a 76-amino-acid protein with a remarkably high degree of conservation between all known sequences. ubiquitin genes are almost always multicopy in eukaryotes, and often are found as polyubiquitin genes--fused tandem repeats which are coexpressed. seventeen ubiquitin sequences from the amitochondrial protist trichomonas vaginalis have been examined here, including an 11-repeat fragment of a polyubiquitin gene. these sequences reveal a number of interesting features that are not seen i ...19957490769
the prevalence of sexually transmitted diseases in children and adolescents evaluated for sexual abuse in cincinnati: rationale for limited std testing in prepubertal girls.to determine the prevalence of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, syphilis, and human immunodeficiency virus (hiv) infection in sexually abused children and to develop selective criteria for sexually transmitted disease (std) testing in these children in our community.19957491226
yi-s, a casein-free medium for axenic cultivation of entamoeba histolytica, related entamoeba, giardia intestinalis and trichomonas vaginalis.pancreatic digests of casein are major ingredients of media used in the axenic cultivation of lumen-dwelling parasitic protozoa, especially entamoeba, giardia, and trichomonads. the digest used almost exclusively in the development of these media, medo-peptone (trypticase bbl), has not been available since 1981. moreover, none of dozens of similar type digests tested since then in our laboratory has proved equal to medo-peptone, and in the last two years it has become increasingly difficult to o ...19957496385
office laboratory diagnosis of vaginitis. clinician-performed tests compared with a rapid nucleic acid hybridization test.the traditional diagnosis of vaginitis incorporates patient symptoms, clinical findings observed during vaginal examination, and laboratory analysis of vaginal fluid. the purpose of this study was to evaluate routine clinician-performed office laboratory diagnostic techniques for women with abnormal vaginal symptoms, and to compare these results with those obtained by a dna hybridization test for trichomonas vaginalis, gardnerella vaginalis, and candida species.19957500067
heterocyclic betaines. xxii. azinium(azolium) 4-nitrobenzimidazolate inner salts and their derivatives with several interannular spacers. synthesis, characterization and antitrichomonal activity.the synthesis of an ensemble of pyridinium(imidazolium) 4-nitrobenzimidazolate betaines and their derivatives with several interannular linkages has been explored. their antiprotozoal activity has also been examined.19957774033
selective elution and purification of living trichomonas vaginalis using gravitational field-flow fractionation.gravitational field-flow fractionation is one of the simplest separation methods for biological materials. its potential in parasitology is demonstrated for trichomonas vaginalis, a parasite responsible for one of the most widespread sexually transmitted diseases. it was observed that this unicellular parasite can be purified in a culture medium with a recovery of 85% for the living trophozoites. the parasite retention characteristics were different when motile living and non-motile dead cells w ...19957780601
sequence of cdna coding for a 65 kda adhesive protein for the specific detection of trichomonas vaginalis by pcr.a trichomonas vaginalis cdna library was constructed and recombinant plaques were screened using rabbit immunoglobulins specific for p65, a protozoan protein involved in pathogenicity that we identified in a previous study. a 1.38 kilobases cdna fragment coding for the p65 protein was cloned in e. coli and then sequenced. on the basis of of the sequence obtained, six primers were synthesised and used to set up a polymerase chain reaction. the presence of a specific amplicon in all 30 clinical is ...19957781986
neonatal trichomonas vaginalis infection.clinical infection with trichomonas vaginalis (t. vaginalis) in the neonate is an unusual occurrence. we present a case of t. vaginalis in a 2-week-old girl with vaginal discharge. our patient had complete resolution of symptoms after treatment with metronidazole. we found few references in the medical literature on the treatment of this condition. this report discusses the pathogenesis and treatment of t. vaginalis in the neonate.19957782625
[in vitro effect of metronidazole ester on trichomonas vaginalis and entamoeba histolytica].metronidazole ester, a new nitroimidazole drug, was synthesized by the department of physical chemistry, school of pharmacy, shanghai medical university. the trichomonadicidal and amoebicidal activity of metronidazole ester was evaluated in vitro by the cultivation of trichomonas vaginalis and entamoeba histolytica at different drug concentrations. the results indicated that the 100% lethal concentration for t. vaginalis and e. histolytica was 2.6 micrograms/ml and 40 micrograms/ml, respectively ...19957788891
[effects of different fixatives for trichomonas vaginalis in indirect fluorescent antibody test].this paper dealt with the effects of different fixatives for trichomonas vaginalis in indirect fluorescent antibody test. t. vaginalis fixed by formalin, methanol or alcohol displayed clear flagella. the parasites fixed by methanol and alcohol showed specific ring-like fluorescence with 1-2 bright dots. antigen slides prepared from different trichomonas strains, different number of parasites and anti-trichomonas vaginalis mcab strains in ifa presented different results. antigen slides showed the ...19957788900
drug therapies for sexually transmitted diseases. clinical and economic considerations.sexually transmitted diseases (stds) are common, and result in immense social and economic costs. in some countries they have a major demographic impact. because many stds facilitate the transmission of hiv, the consequences of stds are further increasing. at the same time, this association between stds and hiv provides one of the ways in which drug therapy should be very cost effective. the perspective taken in this article is a societal one, and broader issues than those directly related to dr ...19957789285
influence of humoral immunity on leukotriene b4 production by neutrophils in response to trichomonas vaginalis stimulation.neutrophils are the predominant inflammatory cells found in vaginal discharges from patients with trichomonas vaginalis infection. in this study, we investigated the effect of humoral immunity on leukotriene b4 (ltb4) generation by neutrophils in the inflammatory response of vaginal trichomoniasis. as quantitated by a radioimmunoassay, no release of ltb4 was detected from neutrophils (5 x 10(6)/ml) interacted with trichomonads (1 x 10(6)/ml). however, specific immunoglobulin g(igg) but not f(ab' ...19957792096
parasite-induced permeation of nucleosides in plasmodium falciparum malaria.a mechanism which mediates the transport of the nonphysiological nucleoside, l-adenosine, was demonstrated in plasmodium falciparum infected erythrocytes and naturally released merozoites. l-adenosine was not a substrate for influx in freed intraerythrocytic parasites or in normal human erythrocytes nor was l-adenosine transported in a variety of cell types including other parasitic protozoa such as crithidia luciliae, trichomonas vaginalis, giardia intestinalis, or the mammalian cells, buffalo ...19957794964
association between reproductive tract infections and cervical inflammatory epithelial changes.to determine the association, if any, between different reproductive tract infections and cervical inflammatory epithelial changes.19957709322
the involvement of terminal carbohydrates of the mammalian cell surface in the cytoadhesion of trichomonads.in the present study the parental cells and glycosylation mutants of chinese hamster ovary (cho) cells were used to analyze the influence of surface carbohydrates on the cytoadhesion of trichomonads. trichomonas vaginalis and tritrichomonas foetus were allowed to interact with host cells for 2 h at 37 degrees c. alternatively, cho cells were treated with 10 mm periodate prior to the assays. both trichomonads adhered to all cho cell clones tested. a remarkable difference could be observed between ...19957731918
leucotriene b4 levels in the vaginal discharges from cases of trichomoniasis. 19957741600
self-administered vaginal swabs for population-based assessment of trichomonas vaginalis prevalence. 19957815873
comparisons of ribosomal rna sequences from amitochondrial protozoa: implications for processing, mrna binding and paromomycin susceptibility.the amitochondrial (a-mt) protozoa include four groups of organisms that are of interest as important human parasites and as probable descendents of the earliest branches of eukaryotic evolution. these organisms have not been directly compared in terms of structure and function of a specific molecule. we sequenced portions of their rrna-encoding genes coding for the internal transcribed spacers (its1 and 2) and adjoining small subunit (ss), 5.8s and large subunit (ls) rrnas. included are sites f ...19957828924
Refresh
firstprev
...21222324252627282930...
nextlast
Displaying items 2601 - 2700 of 4838