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proteus syndrome: a case report.proteus syndrome is a rare genetic disorder, characterized by partial gigantism of the hands and/or feet, asymmetry of the limbs, plantar hyperplasia, multiple hamartomatous subcutaneous tumors, hyperostoses, and long bone overgrowth. a one day old thai male infant is reported with macrosomia, hemihypertrophy of the left side of the face and left leg, large feet, macrodactyly of toes, plantar hyperplasia, large subcutaneous mass with a violet-red surface over the left side of the chest wall and ...200111560226
bioactive compounds from iostephane heterophylla (asteraceae).the novel bisabolene sesquiterpenes 3-6, were isolated from iostephane heterophylla, using bioguided fractionation. the new compounds were determined to be (12r/12s)-12,13-epoxy-xanthorrhizols (3,4) and (12r/12s)-12,13-dihydro-12,13-dihydroxy-xanthorrizols (5,6) and their structures were characterized by analysis of spectroscopic data and by chemical correlation from xanthorrhizol (2). the stereochemistry at c-12 of 5 was deduced using the modified mosher experiment. some of the isolated compoun ...200111561451
ligand diffusion in the catalase from proteus mirabilis: a molecular dynamics study.the role of the channels and cavities present in the catalase from proteus mirabilis (pmc) was investigated using molecular dynamics (md) simulations. the reactant and products of the reaction, h(2)o(2) -->1/2 o(2) + h(2)o, catalyzed by the enzyme were allowed to diffuse to and from the active site. dynamic fluctuations in the structure are found necessary for the opening of the major channel, identified in the x-ray model, which allows access to the active site. this channel is the only pathway ...200111567083
scoliosis in proteus syndrome: case report.the case of patient with scoliosis based on a rare hamartomatous, proteus syndrome, is reported.200111568716
gram-negative bacterial toe web infection: a survey of 123 cases from the district of cagliari, italy.foot intertrigo is mostly caused by dermatophytes and yeasts, less frequently by gram-positive and gram-negative bacteria. nevertheless, the importance of polymicrobial infections and especially colonizations of pseudomonas aeruginosa can cause therapy problems in relation to antibiotic resistance and the risk of potentially lethal complications.200111568743
[clinical significance of microbiological monitoring of infective agents in an urological hospital in the selection of antibacterial therapy regimens].individual microbiological examination of each patient can improve significantly the response of urological patients to antibacterial therapy. 6000 cultures of opportunistic microorganisms isolated from patients with various infectious-inflammatory diseases were studied. most of them were gram-negative: p. aeruginosa, e. coli, enterobacter spp., proteus spp. of gram-positive most frequently occurred staphylococcus spp and enterococcus faecalis. the cultures showed different spectrum of the sensi ...200111569227
[formation of intestinal microflora in children during the first year of their life].in 525 young children the state of intestinal microbiocenosis was studied every month of the first year their life. the study revealed that the process of the microflora formation lasted throughout the first year of their life and was characterized by dysbiotic disturbances. during this period the aggravation of dysbiotic changes in the intestine of these children on months 3, 6-7 and 11-12 was of particular importance. the formation of stable dysbacteriosis led to a decrease in the immunologica ...200111569262
electrochemical biosensor array for the identification of microorganisms based on lectin-lipopolysaccharide recognition.rapid identification of bacterial strains remains a well-known problem in applied medicine and, for viable pathogens, is an important diagnostic goal. we have investigated an electrochemical biosensor array, in which transduction is based on respiratory cycle activity measurements, where the microorganism's native respiratory chain is interrupted with non-native external oxidants. the selective biochemical recognition agents employed in this study are lectins that, once immobilized, recognize an ...200111569815
bacterial swarming: a biochemical time-resolved ftir-atr study of proteus mirabilis swarm-cell differentiation.fourier transform infrared spectroscopy was applied to the study of the differentiation process undergone by proteus mirabilis. this bacterium exhibits a remarkable dimorphism, allowing the cells to migrate on a solid substratum in a concerted manner yielding characteristic ring patterns. we performed an in situ noninvasive analysis of biochemical events occurring as vegetative cells differentiate into elongated, multinucleate, nonseptate, and hyperflagellated swarm cells. the major findings ari ...200111570895
[monitoring of uropathogens and their susceptibility to antibiotics].samples of urine collected from patients with complicated urology infection and hospitalized to the moscow region research clinical institute in 1986, 1991, 1995 and 1999 were analysed. of 11,444 samples examined, bacteriuria was estimated in 7143 samples. 9786 strains (29 genus) of bacteria were isolated--56.9 per cent as mono culture and 43.1 per cent as associations. susceptibility to 21 antibiotic was determined by disk diffusion method for 1607 strains; beta-lactamase production was determi ...200111573319
[clinical significance of infection pathogen resistance in a urological clinic for selection of antibacterial therapy regimes for the treatment of complicated urinary infections].data on changes of etiological structure of the complicated urinary tract infections treated at the research institute of urology and hospital no. 47 at the 5-year period (1996-2000) are given. it was shown that the most important microorganisms were the following: enterobacter spp., e. coli, proteus spp., staphylococcus spp., enterococcus faecalis. the correlation between frequency of the microorganisms isolation and their susceptibility to antimicrobial agents at different periods was analysed ...200111573320
rheumatoid arthritis: the future is not yet now. 200111574984
rheumatoid arthritis as an autoimmune disease caused by proteus urinary tract infections: a proposal for a therapeutic protocol. 200111574986
enzymatic characterization of tem-63, a tem-type extended spectrum beta-lactamase expressed in three different genera of enterobacteriaceae from south africa.the extended-spectrum beta-lactamase, tem-63, was identified in three separate genera of south african isolates: proteus mirabilis, klebsiella pneumoniae, and escherichia coli. this paper describes identification of the gene in these isolates and compares relative rates of hyrolysis between tem-63 and other known ceftazidimases.200111576794
isolation and structure elucidation of a new antifungal and antibacterial antibiotic produced by streptomyces sp. 201.an antibacterial and antifungal antibiotic was isolated from the culture filtrate of streptomyces sp. 201, and its structure was determined as 2-methyl-heptyl isonicotinate by extensive use of nmr spectroscopy. the compound exhibited marked antimicrobial activity against bacillus subtilis, shigella sp., klebsiella sp., e. coli, proteus mirabilis, and the pathogenic fungi, fusarium moniliforme, f. semitectum, f. oxysporum, f. solani and rhizoctonia solani.200111577728
proteus syndrome.a case of proteus syndrome is presented, in which severe hemihypertrophy of the left trunk and left lower extremity, scoliosis, endometriosis and huge bizarre-shaped body tumors were observed. up to 22.6 kg of tumorous tissue was excised. this syndrome was first described in 1983. the name proteus comes from a greek mythical sea god who was able to change his body form freely. this syndrome has numerous features including hemihypertrophy, macrodactyly, various subcutaneous masses, scoliosis and ...200111586023
[massive pleural empyema caused by pyonephrosis].the pleural empyema has unusual origin at extralung causes. then, is essential to identify the primary process in order to obtain the clinical setting control. previously described, but even exceptional, this case report show an empyema in which it's origin was an asymtomatic pyonephrosis, caused because of a big kidney calculus and it's discovery was suspected by the bacillus results. the treatment must include appropriate antibiotic therapy and thoracic drainage, and also the drain of the puru ...200111594181
herpes simplex virus type 2 modulates the susceptibility of human bladder cells to uropathogenic bacteria.the present study analyses the susceptibility of human bladder-derived cells (ht-1376) to the infection by herpes simplex virus type 2 (hsv-2) and chlamydia trachomatis, as well as to the adhesiveness of uropathogenic bacteria. ht-1376 cells were efficiently infected by hsv-2 strain 333, as demonstrated by immunofluorescence staining of viral antigens, titration of cytopathic effect, and visualisation by transmission electron microscopy. this cell model was also prone to c. trachomatis (serovar ...200111599790
[identification of vibrio spp bacteria on skin lesions of fisherman in the county of raposa-ma].the study was undertaken aiming at identifying bacteria from the county of raposa in the state of maranhão. the clinical sample was collected by using a swab and held in a cary-blair transport medium. enrichment in alkaline peptone water, isolation in tcbs selective indicator medium and biochemical coding of species were used for laboratory processing. fifty fisherman with age varying from 12-65 years took part on the study. vibrio bacteria isolated in 21 subjects had been identified. there was ...200111600905
identification of bacteria crucial to histamine accumulation in pacific mackerel during storage.bacterial growth and histamine formation in pacific mackerel during storage at 0, 4, 15, and 25 degrees c were monitored. to identify bacterial species contributing to histamine formation, several groups of bacteria were isolated by using selective media under temperatures corresponding to the various storage conditions. initially, low counts of bacteria were found in the gill, skin, and intestine of fresh fish, and only weak histamine formers were found in the gill. histamine was found in the m ...200111601705
zapa, a possible virulence factor from proteus mirabilis exhibits broad protease substrate specificity.the opportunistic bacterium proteus mirabilis secretes a metalloprotease, zapa, considered to be one of its virulence factors due to its iga-degrading activity. however, the substrate specificity of this enzyme has not yet been fully characterized. in the present study we used fluorescent peptides derived from bioactive peptides and the oxidized beta-chain of insulin to determine the enzyme specificity. the bradykinin- and dynorphin-derived peptides were cleaved at the single bonds phe-ser and p ...200111668347
antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections.isolates from urine samples obtained during 1999 were identified and their susceptibility to antimicrobial agents studied along with any production of extended-spectrum beta-lactamases (esbl) by escherichia coli and klebsiella pneumoniae. a total of 13774 samples were analysed using an automatic system for the detection of bacterial atp (coral, usa). of these samples, 49% were reported to be positive and uncontaminated; bacteria most frequently isolated were e. coli (47%), proteus mirabilis (7%) ...200111673032
a european perspective on nosocomial urinary tract infections i. report on the microbiology workload, etiology and antimicrobial susceptibility (esgni-003 study). european study group on nosocomial infections.to obtain information on the microbiology workload, etiology and antimicrobial susceptibility of urinary tract infection (uti) pathogens isolated in european hospitals.200111683792
epidermal naevus and segmental hypermelanosis associated with an intraspinal mass: overlap between different mosaic neuroectodermal syndromes.the epidermal naevus syndrome is a neurocutaneous syndrome characterised by the association of epidermal naevi with central nervous system, skeletal, ocular or cardiovascular abnormalities. we report on a 1.75-year-old boy who presented with spastic diparesis, a partial paresis of the left forearm and macrocephaly. he had a large epidermal naevus along the cervical spine and a segmental hypermelanosis. mri studies revealed a large intraspinal mass extending from the lower cervical to the upper t ...200111686504
evaluation of extracts of the root of landolphia owerrience for antibacterial activity.ethanolic and aqueous (cold and hot) extracts of landolphia owerrience root parts (whole-root, root-bark and root-wood) were tested for activity against ten bacterial strains using agar-well diffusion and macro-broth dilution methods, respectively. the ethanolic extracts of the whole-root and root-wood were active against 100 and 80% of the test organisms, respectively. ethanolic and aqueous extracts of the root-bark were moderately active while the aqueous (cold and hot) extracts of the root-wo ...200111694355
formation of a tyrosyl radical intermediate in proteus mirabilis catalase by directed mutagenesis and consequences for nucleotide reactivity.proteus mirabilis catalase (pmc) belongs to the family of nadph binding catalases. the function of nadph in these enzymes is still a matter of debate. this study presents the effects of two independent phenylalanine mutations (f194 and f215), located between nadph and heme in the pmc structure. the phenylalanines were replaced with tyrosines which we predicted could carry radicals in a nadph-heme electron transfer. the x-ray crystal structures of the two mutants indicated that neither the bindin ...200111695923
cellular membrane associated mucins in artificial urine as mediators of crystal adhesion: an in vitro enterocystoplasty model.cells are a major component of mucus in enterocystoplasties. we evaluate the role of secreted mucins on cells and cellular membranes as crystal adhesion and agglomeration mediators in artificial urine infected with proteus mirabilis.200111696777
analyses of mrp genes during myxococcus xanthus development.myxococcus xanthus is a gram-negative soil bacterium that undergoes development under starvation conditions. our previous study identified a new genetic locus, mrp, which is required for both fruiting body formation and sporulation. the locus encodes two transcripts: mrpab, which consists of a histidine kinase and an ntrc-like response regulator, and mrpc, a cyclic amp receptor protein family transcription activator. in this study, we used genetic and biochemical analyses to investigate the poss ...200111698359
examination of pineal indoles and 6-methoxy-2-benzoxazolinone for antioxidant and antimicrobial effects.oxidative modification of low density lipoprotein (ldl) induced by free radicals is implicated in the development of atherosclerosis. the aim of the present study was to examine the ability of various pineal indoles in inhibiting ldl oxidation which is accompanied by an increase in mobility in agarose gel electrophoresis and by an augmented generation of thiobarbituric acid-reactive substance induced by cu2+. it was found that the order of potencies in inhibiting malondialdehyde formation was 5- ...200111701394
proteus syndrome associated with ocular anomalies. 200111703003
predominant bacterial agents of childhood septicaemia in jos.blood cultures were performed on 891 children (aged 1 month-16 years) who presented at the jos university teaching hospital (juth) with presumed diagnosis of septicaemia, over a three year period (january 1994-december 1996) in order to determine the predominant bacterial agents. one hundred and thirty-nine (15.6%) out of 891 blood cultures were positive. five of the blood cultures yielded double isolates. staph. aureus was the commonest bacterial agent isolated and accounted for 36.0%, of all t ...200111705063
[cefepime (maxipime), large spectrum 4th generation cephalosporin, resistant to beta-lactamases].as a result of the appearance of bacterial strains resistant to 3rd generation cephlosporin, since 1993 cephalosporins of 4th generation have been developed and introduced in therapy; among them are: cefepime and cefpirome. cefepime is the most active 4th generation cephalosporin possessing the following advantages over the 3rd generation cephalosporins: high intrinsic potency due to rapid penetration into the periplasmic space; an extended spectrum of activity that includes many gram-positive a ...200112731231
using vac to treat a vascular bypass site infection. 200112964221
antimicrobial resistance patterns among urine isolates from patients in a geriatric hospital and from older patients in a general hospital in jerusalem.urinary tract infection is the most common cause of nosocomial infections in patients hospitalized in chronic care facilities. the changing spectrum of microorganisms involved in urinary tract infections and the emerging resistance require continuous monitoring to provide crucial information to guide empirical therapy and encourage prudent use of antibiotics. the aim of this survey was to assess the antimicrobial resistance profile of urine isolates from patients hospitalized in a geriatric hosp ...200112812604
the structure of the carbohydrate backbone of core-lipid a region ofthe lipopolysaccharides from proteus mirabilis wild-type strain s1959 (serotype o3) and its ra mutant r110/1959.the following structure of core-lipid a region of the lipopolysaccharide (lps) from proteus mirabilis strain 1959 (serotype o3) and its rough mutant r110/1959 (proteus type ii core) was determined using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of lps, and of the products of alkaline deacylation of the lps: incomplete substitutions are indicated by italics. all sugars are in pyranose form, alpha-hep is the residue lglycero-alpha-dmanno-hep, alpha-dd- ...200010601875
production of a tem-24 plasmid-mediated extended-spectrum beta-lactamase by a clinical isolate of pseudomonas aeruginosa.several pseudomonas aeruginosa strains, including one urinary isolate producing an extended-spectrum beta-lactamase tem-24, were isolated from a long-term-hospitalized woman. three tem-24-producing enterobacterial species (enterobacter aerogenes, escherichia coli, and proteus mirabilis) were isolated from the same patient. tem-24 and the resistance markers for aminoglycosides, chloramphenicol, and sulfonamide were encoded by a 180-kb plasmid transferred by conjugation into e. coli hb101.200010602754
convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds.a simple disk diffusion test was constructed for detection of imp-1-type metallo-beta-lactamase-producing gram-negative bacteria. two kirby-bauer disks containing ceftazidime (caz) and a filter disk containing a metallo-beta-lactamase inhibitor were used in this test. several imp-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and edta were evaluated for this test. two caz disks were placed on a mueller-hinton agar plate on which a bacterial suspension ...200010618060
immediate and long-term galvanotactic responses of amoeba proteus to dc electric fields.the long-term and immediate galvanotactic responses of amoeba proteus to the direct current electric fields (dcefs) were studied with the methods of computer-aided image analysis. it was found that in contrast to earlier reports, amoebae continued locomotion towards cathode (the negative pole) for hours and the increase in the field strength in the range 300-600 mv/mm caused the straightening of cell trajectories accompanied by the decreased frequency of the lateral pseudopods formation and less ...200010618163
introduction of a mini-gene encoding a five-amino acid peptide confers erythromycin resistance on bacillus subtilis and provides temporary erythromycin protection in proteus mirabilis.a 15-bp mini-gene was introduced into bacillus subtilis and into stable protoplast-like l-forms of proteus mirabilis. this mini-gene encoded the peptide mvlfv and modeled a fragment of escherichia coli 23s rrna responsible for e. coli erythromycin (ery) resistance. expression of the introduced mini-gene conferred permanent ery resistance on b. subtilis. in l-forms of p. mirabilis, the ery-protective effect was maintained in the course of several generations. herewith, the mechanism of ery resist ...200010620668
synthesis of cefminox by cell-free extracts of streptomyces clavuligerus.in vitro synthesis of cefminox by cell-free extracts of streptomyces clavuligerus was investigated using alpha-ketoglutarate, l-ascorbic acid, feso(4), s-adenosyl-l-methionine, and 7alpha-demethoxycefminox as the substrates. the formation of cefminox was detected both by a biological assay with proteus vulgaris gn 76/c-1 and by high performance liquid chromatography. although the conversion rate of 7alpha-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the poten ...200010620685
aortic ring abscess and aortoatrial fistula complicating fulminant prosthetic valve endocarditis due to proteus mirabilis. 200010625192
insertional transposon mutagenesis by electroporation of released tn5 transposition complexes.dna transposition is an important biological phenomenon that mediates genome rearrangements, inheritance of antibiotic resistance determinants, and integration of retroviral dna. transposition has also become a powerful tool in genetic analysis, with applications in creating insertional knockout mutations, generating gene-operon fusions to reporter functions, providing physical or genetic landmarks for the cloning of adjacent dnas, and locating primer binding sites for dna sequence analysis. dna ...200010625401
dynamic aspects of the structured cell population in a swarming colony of proteus mirabilis.proteus mirabilis forms a concentric-ring colony by undergoing periodic swarming. a colony in the process of such synchronized expansion was examined for its internal population structure. in alternating phases, i.e., swarming (active migration) and consolidation (growth without colony perimeter expansion), phase-specific distribution of cells differing in length, in situ mobility, and migration ability on an agar medium were recognized. in the consolidation phase, the distribution of mobile cel ...200010629184
role and mechanism of action of c. pvuii, a regulatory protein conserved among restriction-modification systems.the pvuii restriction-modification system is a type ii system, which means that its restriction endonuclease and modification methyltransferase are independently active proteins. the pvuii system is carried on a plasmid, and its movement into a new host cell is expected to be followed initially by expression of the methyltransferase gene alone so that the new host's dna is protected before endonuclease activity appears. previous studies have identified a regulatory gene (pvuiic) between the dive ...200010629196
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 63 from a new serogroup o68.lipopolysaccharide of proteus penneri strain 63 was degraded by mild acid to give a high molecular mass o-specific polysaccharide that was isolated by gel-permeation chromatography. sugar and methylation analyses and nmr spectroscopic studies, including two-dimensional 1h, 1h cosy, tocsy rotating-frame noe spectroscopy, h-detected 1h,13c and 1h,31p heteronuclear multiple-quantum coherence (hmqc), and 1h, 13c hmqc-tocsy experiments, demonstrated the following structure of the polysaccharide: wher ...200010632731
rapid turnover of flhd and flhc, the flagellar regulon transcriptional activator proteins, during proteus swarming.the enterobacterial flhdc master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. during proteus mirabilis differentiation into elongated hyperflagellated swarm cells, flhdc transcription is strongly but transiently increased. we show that concentration of the flhd and flhc proteins is also tightly controlled at the posttranslational level. this is achieved by protein degradation, which is most severe after differentiation when the half-lif ...200010633123
how do intestinal t cells sense the dietary and microbial environment? 200010648476
fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes.intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ...200010648538
biosynthetically diverse compounds from a saltwater culture of sponge-derived aspergillus niger.the new compound, asperic acid (1), and the known compounds hexylitaconic acid (2), malformin c (3), pyrophen (4), and asperazine (5) were isolated from the saltwater culture of aspergillus niger derived from a caribbean sponge, hyrtios proteus. the structure elucidation of asperic acid is presented.200010650076
structure of a glycerol teichoic acid-like o-specific polysaccharide of proteus vulgaris o12.a phosphorylated o-specific polysaccharide (o-antigen) was obtained by mild acid degradation of proteus vulgaris o12 lipopolysaccharide and studied by sugar and methylation analyses, 1h-, 13c- and 31p-nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, h-detected 1h, 13c and 1h, 31p heteronuclear multiple-quantum coherence experiments. it was found that the polysaccharide consists of pentasaccharide repeating units connected via a glycerol phosphate group, and has the following struc ...200010651815
structure of the o-specific polysaccharide of proteus penneri 71 and classification of cross-reactive p. penneri strains to a new proposed serogroup o64.a neutral o-specific polysaccharide (o-antigen) was isolated from the lipopolysaccharide (lps) of the bacterium proteus penneri 71. on the basis of sugar analysis and 1h- and 13c-nmr spectroscopic studies, including two-dimensional cosy, 13c,1h heteronuclear cosy and roesy, the following structure of the trisaccharide repeating unit of the polysaccharide was established: -->3)-beta-d-glcpnac-(1-->4)-beta-d-glcpnac-(1-->3)-alpha-d-galp-(1-- > the polysaccharide has the same carbohydrate backbone ...200010651818
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 8 from a new serogroup o67.an acidic o-specific polysaccharide was obtained by mild acid degradation of the proteus penneri 8 lipopolysaccharide and found to contain d-glucose, d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2,6-dideoxy-l-galactose (l-fucnac) and 2-aminoethyl phosphate (petn) in the ratios 2 : 1 : 1 : 1 : 1 : 1. 1h and 13c nmr spectroscopy was applied to the intact and dephosphorylated polysaccharides, and the following structure of the hexasaccharide repea ...200010651819
functional morphology of the inner ear and underwater audiograms of proteus anguinus (amphibia, urodela).octavolateral sensory organs (auditory and lateral line organs) of cave salamander proteus anguinus are highly differentiated. in the saccular macula of the inner ear the complex pattern of hair cell orientation and the large otoconial mass enable particle displacement direction detection. additionally, the same organ, through air cavities within the body, enables detection of underwater sound pressure changes thus acting as a hearing organ. the cavities in the lungs and mouth of proteus are a r ...200010653179
the ultrastructure of photoreceptor cells in the pineal organ of the blind cave salamander, proteus anguinus (amphibia, urodela).we studied ultrastructure of the photoreceptor cells in the pineal organ of blind, depigmented, neotenic cave salamander, proteus anguinus. unlike in epigean vertebrates the outer segments of most photoreceptor cells consists of concentrically arranged lamellae, however; in few cells, the outer segments contain 7-9 plasma membrane disks. in both types of photoreceptor cells the outer segments enclose lumps of vesicles of different sizes. the photoreceptor cells of proteus anguinus are similar to ...200010653183
problems related to determination of mics of oximino-type expanded-spectrum cephems for proteus vulgaris.during in vitro susceptibility testing of clinical isolates of proteus vulgaris, we noted that the mics of several expanded-spectrum cephems were much higher in the broth microdilution method than in the agar dilution method (termed the mic gap phenomenon). here we investigated the mechanism of the mic gap phenomenon. cephems with the mic gap phenomenon were of the oximino type, such as cefotaxime, cefteram, and cefpodoxime, which serve as good substrates for inducible class a beta-lactamase (cu ...200010655366
antibacterial activity of some folklore medicinal plants used by tribals in western ghats of india.a series of 30 indian folklore medicinal plants used by tribal healers to treat infections, were screened for antibacterial properties at 10 mg/ml concentration by using disc diffusion method against bacillus subtilis, escherichia coli, klebsiella aerogenes, proteus vulgaris, pseudomonas aerogenes and staphylococcus aureus. twenty plant species showed activity against one or more species of bacteria used in this assay; among them the leaf extracts of cassia occidentalis and cassia auriculata exh ...200010661885
biological activities of lipopolysaccharides of proteus spp. and their interactions with polymyxin b and an 18-kda cationic antimicrobial protein (cap18)-derived peptide.the saccharide constituents of lipopolysaccharides (lps) of proteus spp. vary with the strain and contain unique components about which little is known. the biological activities of lps and lipid a from s- and r-forms of 10 proteus strains were examined. lps from all s-form proteus strains was lethal to d-(+)-galactosamine (galn)-loaded, lps-responsive, c3h/hen mice, but not to lps-hypo-responsive c3h/hej mice. p. vulgaris 025 lps evoked strong anaphylactoid reactions in n-acetylmuramyl-l-alanyl ...200010670563
proteus, a technical and management model for aquatic risk assessment of industrial spills.the assessment of risks to the aquatic environment related to industrial installations is a priority in environmental pollution control in the netherlands. major accidents to the surface water such as the sandoz incident, but also the high number of smaller accidents that occur every year has invoked the need for an effective method to assess these risks. two different models have been used in this field in the netherlands over several years. these two software applications, veris and risam were ...200010677674
microbiologic survey of long-term care facilities.we undertook a microbiologic survey of long-term care facilities to categorize bacteria found in cultures of residents. culture and sensitivity data were collected on 566 samples from indwelling bladder catheters, percutaneous gastrostomy tubes, nares, stool, wounds, pressure ulcers, and tracheostomies in 25 nebraska and iowa facilities. information was also collected on resident factors (eg, presence of indwelling urinary catheter, prior antibiotic administration) and institutional variables (e ...200010679131
biochemical sequence analyses of ges-1, a novel class a extended-spectrum beta-lactamase, and the class 1 integron in52 from klebsiella pneumoniae.klebsiella pneumoniae ori-1 was isolated in 1998 in france from a rectal swab of a 1-month-old girl who was previously hospitalized in cayenne hospital, cayenne, french guiana. this strain harbored a ca. 140-kb nontransferable plasmid, ptk1, that conferred an extended-spectrum cephalosporin resistance profile antagonized by the addition of clavulanic acid, tazobactam, or imipenem. the gene for ges-1 (guiana extended-spectrum beta-lactamase) was cloned, and its protein was expressed in escherichi ...200010681329
proteus syndrome and immunodeficiency.a 10 year old boy with proteus syndrome presented with a pericardial effusion of unknown aetiology. immunological investigation revealed low serum igg and iga, accompanied by low levels of specific antibodies to pneumococcal and haemophilus type b polysaccharides. circulating lymphocyte surface marker profile revealed t and b cell lymphopenia. this is the first report of hypogammaglobulinaemia occurring in the proteus syndrome.200010685928
occurrence and antibiotic sensitivity of enterobacteriaceae isolated from a group of jordanian patients with community acquired urinary tract infections.the type and antibiotic sensitivity of urinary tract pathogens may differ in various communities. of 207 isolates recovered from midstream urine specimens collected from a group of patients with community acquired urinary tract infections (uti), 86% were species of enterobacteriaceae. the most frequently recovered pathogens were escherichia coli (82%), klebsiella spp. (7.3%), proteus spp. (6.2%), enterobacter spp. (3.4%) and citrobacter spp. (1.1%). high rates of resistance were found against am ...200010697742
mixed infection in adult bacterial meningitis.12 adult patients suffering from bacterial meningitis caused by mixed infection were identified at kaohsiung chang gung memorial hospital over a period of 13 years (1986-1998), and they accounted for 6.5% (12/184) of our culture-proven adult bacterial meningitis. the 12 cases included seven males and five females, aged 17-74 years. six of the 12 cases had community-acquired infections and the other six had nosocomially-acquired infections. ten of the 12 cases had associated underlying diseases, ...200010697784
evaluation of the discriminatory powers of the dienes test and ribotyping as typing methods for proteus mirabilis.a total of 63 clinical isolates of proteus mirabilis collected over a 19-month period were typed by the dienes test and ribotyping. ribotyping was performed using the fully automated riboprinter microbial characterization system (qualicon, wilmington, del.). isolates that were indistinguishable by the dienes test and/or ribotyping were characterized further by pulsed-field gel electrophoresis (pfge). most of the isolates represented unique strains as judged by the dienes test and ribotyping. for ...200010699000
trends in the susceptibilities of proteus mirabilis isolates to quinolones. 200010702570
ctx-m-type beta-lactamases: an emerging group of extended-spectrum enzymes.ctx-m-type beta-lactamases constitute a novel group of class a beta-lactamases with extended-spectrum properties. they are encoded by transferable plasmids and found in various enterobacteria, mostly salmonella typhimurium, escherichia coli, klebsiella pneumoniae and proteus mirabilis. ctx-m enzymes share extensive sequence similarity with the chromosomal beta-lactamases of klebsiella oxytoca. they efficiently hydrolyze many newer broad-spectrum oximino-beta-lactams including cefotaxime, ceftria ...200010720804
ciprofloxacin in the treatment of chronic bacterial prostatitis: a prospective, non-comparative multicentre clinical trial with long-term follow-up. the german prostatitis study group.the efficacy and safety of ciprofloxacin 500 mg bd for 28 days were assessed in 65 adult males with symptomatic bacterial prostatitis, from eight centres in germany. urine and prostatic secretions were obtained for culture. clinical signs and symptoms were evaluated at 12-18 days during treatment, end of treatment (4-9 days post-treatment), and 1, 3, 6 and 9 months post-treatment. safety was monitored during and at the end of treatment. e. coli was the most frequent pathogen causing infection (3 ...200010720805
nucleotide sequence of the bla(rtg-2) (carb-5) gene and phylogeny of a new group of carbenicillinases.we determined the nucleotide sequence of the bla gene for the acinetobacter calcoaceticus beta-lactamase previously described as carb-5. alignment of the deduced amino acid sequence with those of known beta-lactamases revealed that carb-5 possesses an rtg triad in box vii, as described for the proteus mirabilis gn79 enzyme, instead of the rsg consensus characteristic of the other carbenicillinases. phylogenetic studies showed that these rtg enzymes constitute a new, separate group, possibly ance ...200010722515
comparable endotoxic properties of lipopolysaccharides are manifest in diverse clinical isolates of gram-negative bacteria.in general there is a poor correlation between serum lipopolysaccharide (lps; the biologically active constituent of endotoxin) levels and mortality in septic patients. the objective of this study was to determine if chemical, structural, or biological differences among lps from different clinical isolates of gram-negative bacteria might explain this discrepancy. lps preparations were made using the hot phenol-water extraction method from eight clinical isolates of gram-negative bacteria. as a p ...200010722580
microbial populations and volatile compounds in the 'bone taint' spoilage of dry cured ham.'bone taint' is one of the most important causes of spoilage of dry cured ham. this alteration is characterized by a foul-smelling odour. the microbial population and volatile compounds associated with incipient 'bone taint' were evaluated. enterobacteriaceae species were found at levels of 7.94 x 10(5) cfu g(-1) in spoiled hams and were not detected in unspoiled hams. serratia sp. and proteus sp. are the main organisms characterized. the volatile compounds from the spoiled hams give rise to hig ...200010728563
treatment of gram-negative bacterial meningitis in term neonates with third generation cephalosporins plus amikacin.the aim of this retrospective study was to evaluate the clinical efficacy in terms of mortality and long-term morbidity of third generation cephalosporins and amikacin in combination for the treatment of gram-negative bacterial meningitis in a homogeneous group of neonates. a 15-year experience (1983-1997) with 72 term neonates without central nervous system anomalies and with gram-negative organisms grown in their cerebrospinal fluid treated with the above combination of antibiotics is presente ...200010729716
minimum inhibitory concentration determinations for various antimicrobial agents against 1570 bacterial isolates from turkey poults.minimum inhibitory concentrations (mics) were determined for 1570 bacteria from eight geographic locations (1204 escherichia coli, 231 other enteric gram-negative bacilli [including citrobacter spp., enterobacter spp., klebsiella spp., proteus spp., and salmonella spp.], 31 pseudomonas spp., 18 coagulase-positive staphylococci, 26 coagulase-negative staphylococci, and 55 streptococci and enterococci) by the national committee for clinical laboratory standards broth microdilution procedure. antim ...200010737648
three-year surveillance study of nosocomial bacterial resistance in argentina. the antimicrobial committee; and the national surveillance program (sir) participants group.a national surveillance program (sir) was introduced in 1996 in argentina by the antimicrobial committee of the argentinean society for microbiology to assess bacterial resistance. the present study reports the rates of nosocomial bacterial resistance found by this program.200010737844
proteus syndrome: mri characteristics of plantar cerebriform hyperplasia.proteus syndrome is a rare congenital hamartomatous syndrome with a variety of abnormalities. it shares many features with other congenital hamartomatous disorders, but cerebriform hyperplasia of the soles and the palms is known as a quite distinctive characteristic in the dermatologic literature. the purpose of this case report is to demonstrate the mri features of plantar cerebriform hyperplasia in a 9-year-old boy with known proteus syndrome.200010741500
is rheumatoid arthritis a form of reactive arthritis? 200010743787
infection and rheumatoid arthritis: guilt by association? 200010743788
antimicrobial activity and spectrum of the new glycylcycline, gar-936 tested against 1,203 recent clinical bacterial isolates.the in vitro activity of gar-936, a new semisynthetic glycylcycline, was evaluated in comparison with two tetracyclines and several other antimicrobial agents. a total of 1,203 recent clinical isolates were tested by reference broth or agar dilution methods. among the members of the family enterobacteriaceae, gar-936 was generally two- to four-fold more active than minocycline, and two- to 16-fold more active than tetracycline. all enteric bacilli mic90 results were < or = 4 microg/ml; the excep ...200010744364
antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections in france. multicentre study group.the aim of this study was to determine the distribution and antibiotic susceptibility patterns of bacterial strains isolated from adults with community-acquired urinary tract infections (uti) in france. from december 1996 to march 1997, each of 15 private laboratories in france consecutively collected about 80 non-duplicate strains isolated from adult outpatients with uti, including patients receiving care at home, and tested their susceptibility by the disk diffusion test. a total of 1160 strai ...200010746497
in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin.the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ...200010747819
clinical relevance of proteus mirabilis in hospital patients: a two year survey.a retrospective study was performed on 1072 non-duplicate isolates of proteus mirabilis, taken in the period april 1996 to march 1998, and on 100 patient charts randomly selected during the same period. p. mirabilis isolates accounted for 7.7% of enterobacteriaceae. the isolates were predominantly from urine (70.2%); of the total, 38.0% were penicillinase-producing isolates, 6.9% were extended-spectrum beta-lactamase (esbl)-producing isolates and 3.6% produced inhibitor-resistant beta-lactamase ...200010747835
germline and germline mosaic pten mutations associated with a proteus-like syndrome of hemihypertrophy, lower limb asymmetry, arteriovenous malformations and lipomatosis.germline pten mutations cause cowden syndrome (cs) and bannayan-riley-ruvalcaba syndrome (brr), two hamartoma-tumour syndromes, and somatic pten alterations have been shown to participate, to a greater or lesser extent, in a wide variety of sporadic neoplasia. pten is a tumour suppressor and dual-specificity phosphatase which affects apoptosis via its lipid phosphatase activity in the phosphoinositol-3-kinase and akt pathway as well as inhibiting cell spreading via the focal adhesion kinase path ...200010749983
trends of antimicrobial resistance in bacterial isolates from a small animal referral hospital.a longitudinal, retrospective investigation was made of antimicrobial resistance in bacterial isolates obtained from clinical cases in a small animal hospital between 1989 and 1997. isolates of escherichia coli and staphylococcus species were used as gram-negative and gram-positive indicator organisms, respectively, and the annual prevalence of antimicrobial resistance was calculated for each organism to each of nine (for e coli) and 11 (for staphylococcus species) appropriate antimicrobials, in ...200010706308
[the resistance of recent clinical isolates against isepamycin, other aminoglycosides and injectable beta-lactams].clinical isolates collected from clinical facilities across japan in 1998 were tested against five aminoglycosides and three beta-lactams. the resistance of 50 strains each of methicillin sensitive staphylococcus aureus, methicillin resistant staphylococcus aureus (mrsa), staphylococcus epidermidis, escherichia coli, citrobacter freundii, klebsiella pneumoniae, enterobacter sp., serratia sp., pseudomonas aeruginosa and proteus sp. (p. mirabilis 25 strains and p. vulgaris 25 strains) to the amino ...200010709145
structure of the o-specific polysaccharide of the bacterium proteus mirabilis o29.an o-specific polysaccharide was obtained by mild acid degradation of p. mirabilis o29 lipopolysaccharide (lps) and found to contain 2-acetamido-2-deoxy-d-galactose and d-glucuronic acid (d-glca) in the ratio 3:1. studies of the polysaccharide by 1h- and 13c-nmr spectroscopy including two-dimensional correlation spectroscopy (cosy), total correlation spectroscopy (tocsy), nuclear overhauser effect spectroscopy (noesy), and h-detected 1h,13c-heteronuclear multiple-quantum coherence (hmqc) experim ...200010713543
[an observation on long-term influence of middle ear bacterial infection on inner ear function and systemic immune reaction].to understand whether long-term inner ear heat shock response related to heat shock protein(hsp70) caused by middle ear bacterial infection and the potential influence on inner ear function.200012768776
diabetic foot: presentation and treatment.the aim of this study is to determine the mode of presentation, medical and surgical treatment in diabetic foot.200011500678
plant growth promotion and fungicidal activity in a siderophore-producing strain of proteus sp.a proteus strain inhibited mycelial growth of fusarium oxysporum in vitro. seed bacterization showed significant plant growth promotion and fusarium-wilt suppression activity of phaseolus mungo in a gnotobiotic system. the culture filtrate of this strain exhibited three prominent bands in uv-vis spectra between 300 and 400 nm. the growth promotion assay of the extracted compound against different indicator organisms indicated the production of a compound related to a 2-oxoacid-type siderophore. ...200011501420
antibacterial and molluscicidal activities of the essential oil of chrysanthemum viscidehirtum.the volatile fraction of chrysanthemum viscidehirtum aerial parts, consisting mainly of limonene, beta-farnesene and many oxygenated sesquiterpenes, was screened for activity against 21 microbial strains. this essential oil exhibited activity against all germs tested, in particular salmonella typhi and proteus mirabilis. it also showed molluscicidal activity against bulinus truncatus.200011449503
[clinico-laboratory parallels during celiac disease in children].forty-five patients with celiac disease and 73 with the celiakia syndrome were observed. the clinical picture was identical and was characterized by disordered intestinal absorption. depression of t and b lymphocytes was typical of both forms of the disease. high values of antigliadin iga and igg correlated with the severity of atrophic processes in the small intestinal mucosa. intestinal microflora was characterized by a decrease in the main defense flora and growth of hemolytical and lactonega ...200011486746
[increased antibacterial activity of antibiotics with etonium in vitro].the activity of compositions of antibiotics cefasolin, benzylpenicillin and gentamycin with etonium in respect to museum strains staphylococcus aureus 209, escherichia coli k-12, proteus vulgaris 410, p. mirabilis, pseudomonas aeruginosa 19, klebsiella pneumoniae 5054 and polyresistance strains s. aureus, e. coli, p. mirabilis in vitro was researched. the increase of the compositions activity as a result of synergy in the action of their component 4 up to 4496 times has been established.200011421003
antimicrobial agent resistance in bacterial isolates from patients with diarrhea and urinary tract infection in the sudan.antimicrobial sensitivity tests were performed on four-hundred and ninety-seven bacterial isolates from sudanese patients with diarrhea or urinary tract infections. shigella dysenteriae type i and enteropathogenic escherichia coli showed high resistance rates (percentage of isolates showing antibiotic resistance) against the commonly-used antimicrobial agents: ampicillin, amoxicillin, chloramphenicol, tetracycline, cotrimoxazole, nalidixic acid, sulfonamide, and neomycin, and were completely sen ...200011421374
microbiology of chronic suppurative otitis media with cholesteatoma.to report the result of causative organisms in chronic suppurative otitis media with cholesteatoma.200011369953
[xanthogranulomatous pyelonephritis. our experience and review of the literature].ten cases of xanthogranulomatous pyelonephritis (xgp) with diagnostic and therapeutic observation are described and other experiences reported in the literature are analyzed.200011315325
pemphigus vulgaris and disseminated nocardiosis.infectious diseases, in particular septicaemia from staphylococcus aureus, proteus vulgaris and pseudomonas aeruginosa, are the most severe and frequent complications for the immunosuppressive therapy of pemphigus. infection by nocardia asteroides in subjects with pemphigus vulgaris is rare. we report the sixth case found of such an association; the subject died of disseminated nocardiosis while receiving steroids and immunosuppressive drugs, 4 years after being diagnosed with chronic pemphigus ...200011305389
bladder washouts in the management of long-term catheters.it has been estimated that 40-50% of patients with long-term catheters can suffer with catheter blockage. this not only causes distress to the patient but also increases the demands on community nurses' time and resources. infection with bacteria such as proteus causes the urine to become alkaline. crystalline deposits can then form inside the catheter lumen which causes blockage. nurses often manage blocked catheters with the use of bladder 'washouts' or bladder instillations. however, the lite ...200011261025
[antibacterial activity of urine after administration of ofloxacin for 5 days].the antibacterial activity of ofloxacin was evaluated in urine over a period of 96 h after oral administration for 5 days of 200 mg twice a day in 12 healthy female volunteers. bacteriostatic and bactericidal activity of urines were studied for five strains of enterobacterias recovered from urinary infections: two strains of escherichia coli nal-s and nal-r, two strains of proteus mirabilis nal-s and nal-r, and one strain of klebsiella pneumoniae nal-s. mean urinary concentrations of ofloxacin w ...200011234464
[diagnostic value of a novel nutrient medium for isolation and cultivation of pathogens causing enteric yersiniosis and pseudotuberculosis].a new nutrient medium for isolation and cultivation of the causative agents of enteric yersiniosis and pseudotuberculosis was found to have advantages over endo medium in its differentiating and inhibiting properties. this medium permitted the easy differentiation of yersinia pseudotuberculosis from y. enterocolitica, as well as from escherichia coli, shigella flexneri, klebsiella pneumoniae, k. rhinoscleromatis, hafnia, enterobacter and citrobacter by color; from proteus inconstans by swarming. ...200011210625
bacteriological study of pus isolates from neuropathic plantar ulcers associated with acute inflammatory phase.in this retrospective study, sensitivity of organisms cultured from ulcers of leprosy patients without and with diabetes mellitus, diabetic patients without leprosy and patients with ulcers from other causes was examined. the profile of organisms grown from these groups of patients did not differ significantly. however, there was a high prevalence of organisms like proteus, e. coli and enterococcus in the ulcers of leprosy patients indicating faecal contamination of the ulcers. co-trimaxazole an ...200011212478
[clinical and laboratory efficacy of the novel difluoroquinolone sparflo (sparfloxacin) in therapy of skin and soft tissue infections].results of clinical trial of new difluoroquinolone--sparflo (sparfloxacin, dr. reddy's laboratories ltd) are presented. sparfloxacin was used in the treatment of 24 patients at the department of wounds and wounds infections (11 patients) and at the department of burn wounds (13 patients) of the a.v. vishnevsky institute of surgery. after the treatment with sparfloxacin pathogen eradication was stated in 18 patients, eradication with superinfection--in 13 patients, persistence--in 3 patients. thu ...200011212495
[electrophoretic forms of glucose-6-phosphate dehydrogenase, acid phosphatase and esterase in amoeba species amoebas].glucose-6-phosphate dehydrogenase (g6pd), acid phosphatase and esterases in free-living amoebae of 7 amoeba species were investigated with the use of disc-electrophoresis in polyacrylamide gel. the evidence provided is suggestive that the electrophoretic isoenzyme patterns of acid phosphatase and esterases (and g6pd in some cases), in addition to a few morphological characters, can serve as a taxonomic criterion for species identification within this genus, as well as for revealing erroneously c ...200011213727
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