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protein a from staphylococcus aureus xi. fixation of human complement and of complement from guinea pig and rabbit. 19714999790
human plasma and the antibacterial effect of peritoneal dialysis solutions.the influence of human plasma on the antibacterial effect of solutions for peritoneal dialysis was studied. the solutions contained 43 meq per litre of either acetate or lactate as the source of base. enough pooled human plasma was added to half of each solution to give a total concentration of a gramme of protein per litre. the numbers of viable organisms from 15 clinical isolates each of staphylococcus aureus, escherichia coli, and pseudomonas species were counted before and after incubation i ...19715541232
the hygiene and marketing of fresh cream as assessed by the methylene blue test. a report by a working party to the director of the public health laboratory service.the hygiene and marketing of fresh cream in england and wales was investigated by a working party of the public health laboratory service (phls) between 1 october 1968 and 31 july 1969. thirty-one cream-producing dairies were visited and observations made in the light of the code of practice published by the milk and milk products technical advisory committee of the ministry of agriculture, fisheries and food, and the scottish home and health department. suggestions are made in this report to st ...19714326247
epicillin: in vitro laboratory studies.a new semisynthetic penicillin, structurally related to ampicillin, has been assigned the generic name epicillin, 6-[d-2-amino-2-(1, 4-cyclohexadienyl) acetamido]-penicillanic acid. the antimicrobial spectrum and level of activity of epicillin in vitro are similar to those of ampicillin. in studies with recent clinical isolates, these two antibiotics, when compared with carbenicillin, showed consistently higher antimicrobial activity against staphylococcus aureus, streptococcus pyogenes, escheri ...19714343402
use of the clumping factor reaction for the identification of encapsulated strains of staphylococcus aureus from human sources.using the clumping factor test on 815 strains of staphylococcus aureus, 775 positive strains were of compact morphology. of 40 negative strains, 39 were diffuse in serum-soft agar. the test may be used to detect capsulated s. aureus strains.197116558041
experimental infection of the skin in the hamster simulating human impetigo. iii. interaction between staphylococci and group a streptococci.the interaction between staphylococci and group a beta hemolytic streptococci in mixed lesions was investigated in an experimental impetigo model. a strain of staphylococcus of phage type 71, which has been shown in vitro to produce a bacteriocin for streptococci and other gram-positive organisms, eliminates or reduces group a streptococci in mixed lesions. in contrast, staphylococcal strains of phage types 75 and 81, which do not produce a demonstrable bacteriocin in vitro, exhibit no such effe ...197115776563
biological and immunological properties of encapsulated strains of staphylococcus aureus from human sources.of 875 strains of staphylococcus aureus isolated from human source clinical specimens, 37 (4.2%) were encapsulated strains. these were all negative for clumping factor and could not be typed with bacteriophages or by serology. twenty-one of these did not produce any hemolysins, 15 produced alpha hemolysin, 1 produced beta hemolysin, and 1 produced both beta and delta hemolysins. after one or two subcultures, 27 of the encapsulated strains converted to the compact variant form, all became positiv ...197016557873
flucloxacillin, a new isoxazolyl penicillin, compared with oxacillin, cloxacillin, and dicloxacillin.flucloxacillin, a new isoxazole penicillin, is active against penicillinase-producing strains of staphylococcus aureus and is well absorbed in man after oral and intramuscular administration. compared with isoxazole penicillins in current clinical use-namely, oxacillin, cloxacillin, and dicloxacillin-flucloxacillin has proved as active against gram-positive cocci, including penicillin-resistant staphylococci. the extent of binding of flucloxacillin to the protein of human serum was similar to th ...19705481218
isolation and partial characterization of a staphylococcal leukocyte cytotaxin.a factor which attracts rabbit polymorphonuclear leukocytes both in vitro and in vivo was isolated from the culture filtrate of a strain of staphylococcus aureus. the activity of the chemotactic factor was independent of fresh serum and it was nondialyzable. incubation of the factor with heat-inactivated human serum markedly inhibited its chemotactic property. the factor was heat-labile (80 c, 10 min) in the crude culture filtrate but was heat-stable when partially purified.19695776515
ammonium sulfate coprecipitation antibody determination with purified staphylococcal enterotoxins.the ammonium sulfate coprecipitation technique of farr was applied in a study of the purified enterotoxins of staphylococcus aureus. ammonium sulfate coprecipitation of iodine-131-labeled enterotoxins a, b, and c, with the use of a 1.6 m concentration of (nh(4))(2)so(4), revealed differences in the antigen-binding capacity of normal and immune rabbit sera for the enterotoxins. the coprecipitation technique provided a quantitative test for detecting antibody to enterotoxin that was more sensitive ...19694979439
modified microbiological assay for rapid estimation of antibiotic concentrations in human sera.antibiotic concentrations in human sera were estimated in 5 to 6 hr by a modified microbiological assay. by using staphylococcus aureus and streptococcus pyogenes as the assay organisms, the seeded assay plates were preincubated for 2 to 6 hr and then were stored at 4 c until used for assay. paper discs saturated with the specimen were placed on the preincubated assay plates with reference discs saturated with known concentrations of antibiotic. after 5 to 6 hr of incubation, zones of antibacter ...19694896101
in vitro antimicrobial activity and human pharmacology of cephaloglycin.serum and urine concentrations of cephaloglycin (an orally absorbed derivative of cephalosporin c) were determined in normal volunteers and in patients. the in vitro activity of cephaloglycin was also studied. all strains of group a streptococci (streptococcus pyogenes) and diplococcus pneumoniae were inhibited by 0.4 mug of cephaloglycin per ml. eighty per cent of the staphylococcus aureus strains and about 50% of the escherichia coli and proteus mirabilis strains were inhibited by 1.6 mug of c ...19684385749
serological activity of protein a of staphylococcus aureus: the precipitinogen as an antigen for determining antibodies by the passive hemagglutination test.tanned sheep erythrocytes have been considered incapable of sensitization with the precipitinogen of protein a of staphylococcus aureus, so that the activity of this antigen in serological reactions has so far been studied by means of the agar diffusion test (adt) only. the precipitinogen of the protein a in this study was found to become attached to the tanned erythrocytes and to sensitize them for the passive hemagglutination test (pht). it was determined that, in contrast to nonspecific react ...19684969604
[on the ecology of staphylococcus aureus on human skin surface. 3. staphylococcus aureus artificially transferred onto normal skin surface of the forearm and other body regions]. 19685678275
cytotoxic potential of stimulated human lymphocytes.viable and immunologically competent lymphocytes from unsensitized donors damage allogeneic tissue culture cells in the presence of phytohemagglutinin (pha). this cytotoxicity is specific since syngeneic tissue culture cells are not at all or only slightly damaged under similar experimental conditions. in this investigation, the relation between the stimulation of human lymphocytes and their cytotoxicity was studied. chang cells (human liver) served as target cells in all experiments. cell damag ...19676020009
opsonizing factors in human serum. ii. the opsonin and inhibitor of phagocytosis of staphylococcus aureus. 19676057642
[on the ecology of staphylococcus aureus on the human skin surface. i. on the phenomenon of the so-called self disinfective power of the skin surface]. 19675599882
effect of streptococcus pyogenes on tissue cells.human tissue cell lines from each of the three primary germinal sources, ectoderm (conjunctiva and carcinoma of the buccal mucosa), entoderm (intestine and liver), and mesoderm (heart and monocytes) were inoculated with group a streptococcus pyogenes, staphylococcus aureus, and group d streptococci and were then observed. in addition, the effect of these bacteria on mouse fibroblasts was studied. all of the cell lines appeared to be equally susceptible to damage, but damage to the cells by s. py ...19675337771
heterogenetic antigens of gram-positive bacteria.chorpenning, frank w. (the ohio state university, columbus), and matthew c. dodd. heterogenetic antigens of gram-positive bacteria. j. bacteriol. 91:1440-1445. 1966.-soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune rabbit sera and normal human sera were then studied. antigens from all gram-positive organisms studied except corynbacteria altered red cells, causing them to react with specific bact ...19664956340
[results of the determination of resistance to corrosive sublimate in staphylococcus aureus strains of human and animal origin]. 19665979659
the use of an antibiotic nasal spray in human nasal carriers of bacteriophage typable, coagulase positive staphylococcus aureus. 19655829271
fate of staphylococcus aureus in bruised tissue.this investigation was initiated to determine the fate and behavior of a virulent marker strain of staphylococcus aureus (ms) injected intramuscularly into both control and traumatized tissues. control tissues appeared to possess a clearing mechanism highly active against this organism, whereas bruised tissues stimulated and supported its growth. this stimulation may be due to the presence of extrastromal hemoglobin in the bruised areas. by use of the disc-sensitivity procedure, extrastromal hem ...196514264842
in vitro phagocytosis of staphylococcus aureus with special reference to a possible antiphagocytic function of coagulase.borchardt, kenneth a. (tulane university school of medicine, new orleans, la.), and william a. pierce, jr. in vitro phagocytosis of staphylococcus aureus with special reference to a possible antiphagocytic function of coagulase. j. bacteriol. 87:311-315. 1964.-rabbit antisera were prepared to staphylococcus aureus strains of bacteriophage types 81 and 42d with vaccines consisting of washed, heat-killed cells grown in media which either did or did not support coagulase formation. partially purifi ...196414151049
phagocytosis and intracellular killing of staphylococcus aureus by human and rabbit blood leukocytes. 196414214395
some physiological charcteristics of two sets of phage-propagating strains of staphylococcus aureus.a number of physiological characteristics were studied on some 29 strains of phage-propagating staphylococci belonging to the basic international series and the seto-wilson bovine-adapted set. all the cultures except strain 73 were coagulase-positive, with reciprocal titers ranging from 2 to 8,192. strain 73 was again an exception with respect to phosphatase activity. group 1 yielded high values for both phosphatase and oxygen uptake but low values for extracellular protein. resistance to penici ...196313978076
characteristics of a strain of staphylococcus aureus grown in vivo and in vitro.beining, paul r. (the catholic university of america, washington, d.c.) and e. r. kennedy. characteristics of a strain of staphylococcus aureus grown in vivo and in vitro. j. bacteriol. 85:732-741. 1963.-a comparative survey was conducted on the characteristics of a strain of staphylococcus aureus after it had been grown in vitro (vsb) and after it had been collected from the peritoneal exudate of an infected guinea pig (gsb). both vsb and gsb strains gave the same results when studied in an ext ...196314044937
dissociation of phagocytosis and intracellular killing of staphylococcus aureus by human blood leukocytes. 196314053299
synergistic activity between pr8 influenza virus and staphylococcus aureus in the guinea pig. 196314071350
further studies on staphylococci in meats. iv. the bacteriophage pattern and antibiotic sensitivity of isolates from nonfrozen meats.of 272 isolates of staphylococcus aureus recovered from 173 samples of 10 market meats from 27 stores, 173 (63.2%) were phage typable, employing 28 phages. sixty per cent of the phage-typable strains belonged to group iii, followed by 14.5% to mixed groups i and iii, 10.4% to group i, 8.7% to all mixed groups, 4.6% to group ii, and 1.7% to group iv. the most commonly recovered patterns were 83, 53/83, and other similar combinations of 53. the nonpigmented strains which did not have bound coagula ...196214451458
the occurrence of staphylococcus aureus in mother's milk. 195914436208
[notes on behavior of anti-influenza antibodies, o antistreptolysin and alpha-antistaphylolysin titers in asiatic influenza patients]. 195813510075
importance of staphylococcus aureus in pneumonia in the 1957 epidemic of influenza a. 195813564806
[micrococcus phogenes var. aureus in human faces]. 195813615796
[absorption, diffusion and elimination of novobiocin in man; in vitro activity in 50 strains of staphylococcus]. 195713418472
the responsibility of spermine for the antibacterial action of human semen. 195713429207
[staphylococcal immune-adhesion reaction in man]. 195713442210
the virulence of staphylococcus pyogenes for man; a study of the problems of wound infection. 195713499821
[bacteriophage typing and antibiotic sensitivity of m. pyogenes strains isolated from various human organs]. 195613369903
staphylococcus pyogenes: the antibiotic sensitivity of strains isolated from human carriers. 195413221813
[studies on the naturally acquired immunity against staphylococcus beta toxin in man and different species of animals]. 195313094182
[the presence of micrococcus pyogenes in human feces]. 195313124827
studies on the pneumococcus : ii. dissolution of pneumococci at varying hydrogen ion concentrations. effect of temperature, previous killing of the organisms, and fresh human serum on the phenomenon. behavior of other organisms.suspensions of living pneumococci in approximately isotonic standard solutions and in approximately isotonic bouillon with ph varying from about 4.0 to 8.0 after incubation show dissolution of organisms in those solutions having a ph higher than about 5.0. dissolution is most marked at a critical range of about ph 5.0 to 7.0. some dissolution also takes place toward the more alkaline end of the scale. no dissolution occurs at the most acid end of the scale. dissolution in the standard solutions ...192219868639
the comparative resistance of bacteria and human tissue cells to certain common antiseptics.the comparative resistance of bacteria and human tissue cells to antiseptics and other chemicals may be easily tested by tissue cultures under conditions which approximate those found in the living body. a comparative study shows that while human cells (connective tissue and wandering cells) are highly resistant to many antiseptics, they are in general more easily killed than bacteria (staphylococcus aureus). of the antiseptics tested, which include mercuric chloride, iodine, potassium mercuric ...191619868066
an experimental study of opsonic immunity to staphylococcus aureus.1. the administration of staphylococcus aureus, killed by heat (vaccine), produces a high degree of opsonic immunity in rabbits. 2. such increase of opsonin affords protection against living virulent staphylococcus in direct proportion to the amount of opsonins present in the serum and complete recovery may follow subsequent inoculation, if the opsonic power be high. 3. frequent administration of vaccines may produce a diminution of the opsonic power of the serum. 4. immune opsonins are most act ...191019867314
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