Publications

[detection of telomerase activity in cervical lesions by non-radioactive telomeric repeat amplification protocol (trap)].	worldwide, the cervical cancer is recognized as the second most common type or malignancy in women. the implementation of the papanicolaou smear has contributed notably to diminish its morbi-mortality. however, this test has a sensibility of just 80%. the detection of telomerase activity (ta) has been proposed as a diagnostic and prognostic maker of a wide variety of tumors and their precursor lesions. in this study the detection of ta was made from samples of cervical exfoliates and its associa ...	2005	16152781
association of human papillomavirus infection with palmoplantar epidermal cysts in korean patients.	human papillomavirus (hpv) infection can develop into many different kinds of diseases ranging from simple warts to carcinoma in situ. some investigators have recently suggested that hpv infection could be closely related to palmoplantar epidermal cysts. the aim of this study was to determine the existence of hpv and its subtype in palmoplantar epidermal cysts of korean patients. we investigated 13 cases of palmoplantar epidermal cysts by employing clinical, histopathological and immunohistochem ...	2005	16159731
synchronous cin 3 and cervical lymphoma: a case report and review of the literature.	primary cervical lymphoma is rare, with less than 60 cases reported in the english literature, including a few cases of synchronous cervical intraepithelial neoplasia (cin) with cervical lymphoma. we describe a 42-year-old woman with cin 3 and malignant lymphoma of the cervix. the papanicolaou cytology only revealed dysplastic cells of cin 3, but not the malignant lymphoid cells. the histology of the cervix revealed diffuse infiltration of monotonous medium-sized lymphoid cells with dispersing t ...	2005	16164048
two distinct activities contribute to human papillomavirus 16 e6's oncogenic potential.	high-risk human papillomaviruses, such as hpv16, cause cervical cancers, other anogenital cancers, and a subset of head and neck cancers. e6 and e7, two viral oncogenes expressed in these cancers, encode multifunctional proteins best known for their ability to bind and inactivate the tumor suppressors p53 and prb, respectively. in skin carcinogenesis experiments using e6 transgenic (k14e6(wt)) mice, hpv16 e6 was found to contribute to two distinct stages in skin carcinogenesis: promotion, a step ...	2005	16166303
characterization of the global profile of genes expressed in cervical epithelium by serial analysis of gene expression (sage).	serial analysis of gene expression (sage) is a new technique that allows a detailed and profound quantitative and qualitative knowledge of gene expression profile, without previous knowledge of sequence of analyzed genes. we carried out a modification of sage methodology (microsage), useful for the analysis of limited quantities of tissue samples, on normal human cervical tissue obtained from a donor without histopathological lesions. cervical epithelium is constituted mainly by cervical keratin ...	2005	16171524
incidence trends of adenocarcinoma of the cervix in 13 european countries.	rapid increases in cervical adenocarcinoma incidence have been observed in western countries in recent decades. postulated explanations include an increasing specificity of subtype-the capability to diagnose the disease, an inability of cytologic screening to reduce adenocarcinoma, and heterogeneity in cofactors related to persistent human papillomavirus infection. this study examines the possible contribution of these factors in relation with trends observed in europe. age-period-cohort models ...	2005	16172231
human papillomavirus infection: biology, epidemiology, and prevention.	over the past several decades, knowledge of the biology and epidemiology of human papillomavirus (hpv) infection has increased tremendously. however, there are still many unanswered questions concerning the interaction of the virus with its host. the virus has been identified as a necessary causal agent for cervical squamous neoplasia and has been linked to the development of neoplasia in several other mucosal sites. the viral oncogenes e6 and e7 are the major players in the virus' scheme to eva ...	2005	16174218
pseudoinfiltrative tubal metaplasia of the endocervix: a potential form of in utero diethylstilbestrol exposure-related adenosis simulating minimal deviation adenocarcinoma.	we report three cases of unusual tubal-type endocervical glandular proliferations simulating minimal deviation adenocarcinoma in women with a history of in utero diethylstilbestrol (des) exposure. the lesions were characterized by haphazard glandular proliferations extending from 3.4 to 6.1 mm into the endocervical stroma and to the margins of excision in all cases. most of the glands were small to medium-sized and round; some exhibited a moderate degree of cystic dilatation, and occasional glan ...	2005	16175088
human papillomavirus 16 minor capsid protein l2 helps capsomeres assemble independently of intercapsomeric disulfide bonding.	the human papillomavirus (hpv) capsomeres (pentamers of major capsid protein l1), which constitute along with l2 the virion capsid, can assemble themselves alone into the l1-capsid particles in vivo and in vitro, depending on intercapsomeric disulfide bonds. to study a possible role of l2 in capsid assembly, we examined the interaction between hpv16 l2 and capsomeres under the conditions that inhibit the formation of disulfide bonds in vitro and in vivo. the purified l2 bound to free capsomeres ...	2005	16175337
correlation between deoxyribonucleic acid loads of human papillomavirus and recurrence of condylomata acuminata.	to determine the correlation between deoxyribonucleic acid (dna) loads of human papillomavirus (hpv) and recurrence of condylomata acuminata (ca), 31 cases of primary ca and 32 cases of recurrent ca were assayed for the hpv6/11 and hpv16/18 dna loads by real-time fluorogenic quantitative pcr. the results showed 62 of the 63 cases were hpv6/11 dna positive (98.4%). the ranges of hpv6/11 dna contained in primary and recurrent ca were 1.4 x 10(3)-6.7 x 10(7) and 1.2 x 10(4)-3.6 x 10(8) copies/ml, r ...	2005	16176626
[inverted naso-sinusal papilloma: analytic study on 13 cases].	inverted nasosinusal papilloma is a rare tumorous process involving mucosa of nasal fossa and paranasal sinus unilaterally. the authors studied inverted naso-sinusal papilloma observed and treated in ent and head and neck department at brazzaville university hospital.	2005	16180352
p53 intronic g13964c variant in colon cancer and its association with hpv.	inactivation of p53 is the most common change identified in human cancer. nucleotide alterations in p53 intron 6 have been reported to be associated with the dysregulation of p53 function and tumor development. the aim of our study was to assess whether the intron 6 g13964c variant of the p53 gene is associated with the presence of human papillomavirus (hpv) as a risk factor in colon cancer.	2005	16080524
the biological role of smoking, oral contraceptive use and endogenous sexual steroid hormones in invasive squamous epithelial cervical cancer.	for at least twenty years, epidemiological studies have found a correlation between cervical neoplasia and smoking and oral contraceptives. over the last ten years, laboratory evidence has supported epidemiological findings. one example is the addition of progesterone to human papillomavirus-transfected cervical cells, which has led to oncogenic cell transformation. very high concentrations of tobacco constituents in cervical mucus, as compared to serum, in smokers provided evidence of a biologi ...	2005	16080563
expression of human papillomavirus type 16 l1 in baculovirus expression systems: a case study.		2005	16082027
optimization and validation of a multiplexed luminex assay to quantify antibodies to neutralizing epitopes on human papillomaviruses 6, 11, 16, and 18.	a human papillomavirus (hpv) multiplexed competitive luminex immunoassay first described by opalka et al. (d. opalka, c. e. lachman, s. a. macmullen, k. u. jansen, j. f. smith, n. chirmule, and m. t. esser, clin. diagn. lab. immunol. 10:108--15, 2003) was optimized and validated for use in epidemiology studies and vaccine clinical trials. optimization increased both the analytical sensitivity and the clinical specificity of the assay to more effectively discriminate the low-titer antibody respon ...	2005	16085914
human papilloma virus papilloma masquerading as adenoid hypertrophy.		2005	16087035
human papillomavirus-related cervical and anal disease in hiv-infected individuals in the era of highly active antiretroviral therapy.	hiv-infected men who have sex with men remain at high risk of developing anal cancer despite the widespread use of highly active antiretroviral therapy (haart). in hiv-infected women, however, there is some evidence that haart may be associated with regression of human papillomavirus (hpv)-related cervical disease. so far, epidemiologic data provided by cancer registries have shown no reduction in the incidence of cervical and anal cancer in patients with hiv infection since the initiation of ha ...	2005	16091261
aerodigestive cancers in hiv infection.	aerodigestive (lung, head and neck) cancers are among the most prevalent and deadly neoplasms worldwide, and the incidence rates are rising. given the improvements in life expectancy of persons with hiv/aids when treated with highly active antiretroviral agents, persons with hiv infection are therefore increasingly likely to develop these malignancies. this chapter focuses on the epidemiology of these malignancies and reviews the most recent literature and current understanding of the causes and ...	2005	16093799
the utility of hpv dna triage in the management of cytological agc.	given the demonstrated utility of human papilloma virus dna triage in the management of atypical squamous cells of undetermined significance, this study sought to evaluate the potential role of human papilloma virus dna testing in the evaluation and management of cytological atypical glandular cells.	2005	16098895
differential splicing of e6 within human papillomavirus type 18 variants and functional consequences.	persistent infections of the uterine cervix with 'high-risk' human papillomavirus (hpv) are now recognized as necessary for the development of cervical cancer. among them, hpv types 16 and 18 exhibit numerous variants associated with different risks for cervical cancer development. in this study, the questions of whether different hpv type 18 variants exhibit changes in early gene transcription and the molecular mechanisms underlying these differences were investigated. it was shown that, indeed ...	2005	16099904
loss of 10p material in a child with human papillomavirus-positive disseminated bilateral retinoblastoma.	retinoblastoma (rb) is a malignant childhood tumor that results from loss or inactivation of both alleles of the rb1 gene. human papillomavirus (hpv) dna sequences have been found in rb tissue, suggesting a role of the viral infection with rb. we here describe a child with disseminated bilateral rb without familial history, who displayed a loss of material from 10p. fluorescence in situ hybridization studies showed a somatic loss of both alleles of the rb1 gene. moreover, sequences for hpv-6a we ...	2005	16102585
[evaluation of search for human papillomavirus (hpv) during cervical precancer and cancer screening: synopsis and perspectives (may 2004)].		2005	16108114
parental acceptance of the human papillomavirus vaccine.	we conducted focus groups and individual interviews to explore parental views on the human papillomavirus (hpv) vaccine. parents were generally positive about the hpv vaccine. some participants perceived their children were not at risk for acquiring hpv and questioned vaccinating young adolescents against hpv. vaccine education should target parental beliefs about hpv and the optimal age for hpv vaccine administration.	2005	16109349
immunotherapy with ctl peptide and vssp eradicated established human papillomavirus (hpv) type 16 e7-expressing tumors.	peptide-based vaccines aimed at the induction of effective t-cell responses against established tumors have not been successful in clinic and require the use of new adjuvants. one of those is a new adjuvant in which gangliosides are incorporated into the outer membrane protein complex of neisseria meningitidis to form very small size proteoliposomes (vssp). in a preclinical model of human papillomavirus hpv16-induced cervical cancer we show that vaccination with hpv 16 e7 derived minimal ctl pep ...	2005	16112257
tissue distribution of human papillomavirus 16 dna integration in patients with tonsillar carcinoma.	human papillomavirus 16 (hpv-16) has been implicated as a causative agent in a subset of head and neck squamous cell carcinomas (hnscc). this study was undertaken to discern the distribution and timing of hpv viral integration during tumorigenesis of the upper respiratory tract.	2005	16115905
profiling estrogen-regulated gene expression changes in normal and malignant human ovarian surface epithelial cells.	estrogens regulate normal ovarian surface epithelium (ose) cell functions but also affect epithelial ovarian cancer (oca) development. little is known about how estrogens play such opposing roles. transcriptional profiling using a cdna microarray containing 2400 named genes identified 155 genes whose expression was altered by estradiol-17beta (e2) in three immortalized normal human ovarian surface epithelial (hose) cell lines and 315 genes whose expression was affected by the hormone in three es ...	2005	16116479
immunotherapy for gynaecological malignancies.	gynaecological malignancies, excluding breast cancer, cause approximately 25,000 deaths yearly among women in the us. therefore, novel approaches for the prevention or treatment of these diseases are urgently required. in the case of cervical cancer, human papillomavirus (hpv) xenoantigens are readily recognised by the immune system, and their targeting has shown great promise in preclinical models of therapeutic vaccination and in clinical studies of preventative vaccination. a growing body of ...	2005	16120050
epidemiologic correlates of cervical human papillomavirus prevalence in women with abnormal pap smear tests: a taiwan cooperative oncology group (tcog) study.	to explore factors affecting human papillomavirus (hpv) prevalence in all grades of cervical neoplasia among chinese women, 1,264 women with abnormal cervical cytology attending the gynaecologic clinics of 11 major medical centres in taiwan. patients were interviewed and underwent complete gynaecologic examination including colposcopy. cervical scrapings were collected for hpv dna detection by both hybrid capture-2 (high-risk probe) and l1 consensus pcr-reverse line blot. the prevalences of hpv ...	2005	16121376
the p53 codon 72 polymorphism and risk of high-grade cervical intraepithelial neoplasia.	the arg/arg genotype versus arg/pro or pro/pro at codon 72 of the p53 gene has been implicated in increasing susceptibility of the cervix to human papillomavirus (hpv) infection and thus altering cancer risk. however, research on this topic has been contentious, which prompted us to carry out a case-control study in the montreal area.	2005	16122882
p53 mutations and human papillomavirus infection in oral squamous cell carcinomas: correlation with overall survival.	the study investigated the pattern of p53 gene mutations and human papillomavirus (hpv) infection concerning their relation to overall survival in patients with oral squamous cell carcinomas of the tongue and floor of the mouth.	2005	16129609
incomplete inhibition of the rb tumor suppressor pathway in the context of inactivated p53 is sufficient for pancreatic islet tumorigenesis.	here, we describe the surprising residual capability of the rb pathway to negatively regulate proliferation and tumorigenesis in a sv40 large t antigen (tag)-driven mouse model of pancreatic islet carcinogenesis. heterogeneous tag expression during all progression stages suggested that a threshold level of the t antigen oncoprotein might be deterministic for beta-cell hyperproliferation and led us to hypothesize that tag might not be fully inhibiting the tumor suppressor activity of rb. moreover ...	2005	16007165
epidemiological study in okinawa, japan, of human papillomavirus infection of the uterine cervix.	to investigate the prevalence and type distribution of human papillomavirus (hpv) in women with normal cervical cytology and with cervical intraepithelial neoplasia i to iii(cin) or carcinoma of the cervix in okinawa, japan.	2005	16011997
knowledge of cervical cancer and screening among women in east-central england.	this study assesses the extent and accuracy of women's knowledge of cervical cancer, risk factors, and the efficacy of the national screening program. data were obtained from a questionnaire survey of randomly selected women eligible for screening, drawn from a population in east-central england. the majority of women in the sample overestimated the current incidence of cervical cancer, both absolutely and relative to other cancers. perceiving incidence to be high was associated with reporting w ...	2005	16014118
[relations between the expression of cyclin e, p16ink4, ki67 and hpv16/18 infection in cervical exfoliated cells].	to confirm the relations between the expression of cyclin e, p16ink4, ki67 and hpv16/18 infection using cervical exfoliated cells, and evaluate the usefulness of cyclin e, p16ink4 and ki67 as biomarkers for screening of cervical carcinomas.	2005	16027780
laser capture microdissection and pcr for analysis of human papilloma virus infection.	human papilloma virus (hpv) infection is considered one of the main factors involved in the pathogenesis of endocervical adenocarcinoma. however, the cellular location of hpv in this type of tumor is controversial. we have developed a method to determine the presence of hpv type 16 in endocervical cancer cells using laser capture microdissection followed by dna extraction and qualitative polymerase chain reaction. our results show that hpv type 16 is present in endocervical adenocarcinoma cells.	2005	16028428
human papillomavirus type 16 infections and 2-year absolute risk of cervical precancer in women with equivocal or mild cytologic abnormalities.	the 2-year absolute risk for cervical precancer attributable to infection by human papillomavirus type 16 (hpv16), the most common and oncogenic hpv type, in the millions of women diagnosed annually with equivocal or mildly abnormal cytology has not been definitively evaluated.	2005	16030304
the elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (hpv) type 16 or 18 and the possible utility of type-specific hpv testing in clinical practice.	human papillomavirus (hpv) types 16 and 18 cause 60%-70% of cervical cancer worldwide, and other hpv types cause virtually all remaining cases. pooled hpv testing for 13 oncogenic types, including hpv16 and 18, is currently used in clinical practice for triage of equivocal cytology and, in conjunction with pap tests, is an option for general screening among women 30 years of age and older. it is not clear to what extent individual identification of hpv16 or hpv18 as an adjunct to pooled oncogeni ...	2005	16030305
presence and type of oncogenic human papillomavirus in classic and in differentiated vulvar intraepithelial neoplasia and keratinizing vulvar squamous cell carcinoma.	the presence and type of oncogenic papillomavirus (hpv) in classic warty/basaloid vulvar intraepithelial neoplasia and in differentiated vulvar intraepithelial neoplasia and keratinizing vulvar squamous cell carcinoma was investigated using three techniques, that is, histology, in situ hybridization, and pcr-elisa. hpv typing was performed using in situ hybridization and pcr-elisa. differentiated vulvar intraepithelial neoplasia and invasive keratinizing vulvar squamous cell carcinoma proved com ...	2005	16032717
candidate human papillomavirus (hpv) type 27b: nucleotide sequence and heterogeneity with hpv 27.	more than 100 human papillomavirus (hpv) types have been reported as tumorigenic agents. hpv types 2 and 27 were identified in benign tumors and classified closely according to the sequence homology. an hpv type 2 related sequence was identified previously in an oral papilloma. however, the correct typing and sequencing was impossible at that time. a candidate hpv was cloned by the long-pcr technique from an oral papilloma and characterized by nucleotide sequence. this virus was basically hpv ty ...	2005	16032718
detection of human papillomavirus (hpv) 16-specific cd4+ t-cell immunity in patients with persistent hpv16-induced vulvar intraepithelial neoplasia in relation to clinical impact of imiquimod treatment.	topical application of the immune response modifier imiquimod is an alternative approach for the treatment of human papillomavirus (hpv)-positive vulvar intraepithelial neoplasia (vin) and aims at the immunologic eradication of hpv-infected cells. we have charted hpv16-specific immunity in 29 patients with high-grade vin and examined its role in the clinical effect of imiquimod treatment.	2005	16033846
activation of the protein kinase b pathway by the hpv-16 e7 oncoprotein occurs through a mechanism involving interaction with pp2a.	protein kinase b (pkb) or akt is one of several second messenger kinases that are activated by cell attachment and growth factor signaling, and that transmit signals to the cell nucleus to inhibit apoptosis and thereby increase cell survival during proliferation. other viral proteins target this pathway by increasing pkb/akt phosphorylation, and this pathway has been implicated in the transformation of human keratinocytes by hpv e6 and e7, together with activated notch 1. here, we examine how hp ...	2005	16044149
hpv infection in adolescents: natural history, complications, and indicators for viral typing.	human papillomavirus (hpv) is the most common sexually transmitted disease in both men and women. prevalence rates are the highest for adolescents. despite the high prevalence rates, sequelae of genital warts, dysplasia, and cancer are rare developments. knowledge about the natural history, virology, and cancerous transformation has lead to improved viral detection, including the use of hpv dna detection tests, screening efforts for hpv-related precancerous and cancerous lesions, and clinical in ...	2005	16044390
proposed sheffield quantitative criteria in cervical cytology to assist the grading of squamous cell dyskaryosis, as the british society for clinical cytology definitions require amendment.	in 1986, the british society for clinical cytology (bscc) published quantitative criteria to assist diagnosis in a three-tier grading system of squamous cell dyskaryosis. in dyskaryotic cells, area nuclear to cytoplasmic (nc) ratios below 50%, between 50% and 66% and over 66% were defined as equating with mild, moderate and severe grades respectively. following the terminology conference in 2002, however, the bscc recommended on their website that the three-tier model should be replaced by a new ...	2005	16048504
immune responses to human papillomavirus in genital tract of women with cervical cancer.	to address a question whether immune responses to hpv infection play a role in control of cervical cancer, we analyzed systemic and mucosal immune responses to hpv in women who underwent radical hysterectomy for cervical cancer (hcc) or loop conization due to cervical dysplasia (loop), or had hysterectomy for other reasons (hnn).	2005	15661235
modification of professional antigen-presenting cells with small interfering rna in vivo to enhance cancer vaccine potency.	rna interference using small interfering rna (sirna) is an effective means of silencing gene expression in cells. intradermal administration of nucleic acids via gene gun represents an efficient method for delivering nucleic acids to professional antigen-presenting cells in vivo. in this study, we show that the coadministration of dna vaccines encoding human papillomavirus type 16 e7 with sirna targeting key proapoptotic proteins bak and bax prolongs the lives of antigen-expressing dendritic cel ...	2005	15665308
determinants of human papillomavirus-negative, low-grade squamous intraepithelial lesions in the atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesions triage study (alts).	although low-grade squamous intraepithelial lesions (lsil) most often are the result of infection by human papillomaviruses (hpv), a small proportion of women with lsil have negative hpv tests. using the atypical squamous cells of undetermined significance/lsil triage study (alts) population, the authors evaluated the significance of hpv-negative lsil.	2005	15991244
colposcopy vs hybrid capture ii assay in detection of cervical human papilloma virus infection.	considering the relationship between high-risk human papillomavirus types and the presence or subsequent development of cervical high-grade preinvasive lesions, the aim of the study was to determine if the hybrid capture ii test can be used to triage women with atypical colposcopic findings.	2005	15991532
involvement of nuclear export in human papillomavirus type 18 e6-mediated ubiquitination and degradation of p53.	the e6 protein from high-risk human papillomaviruses (hpvs) targets the p53 tumor suppressor for degradation by the proteasome pathway. this ability contributes to the oncogenic potential of these viruses. however, several aspects concerning the mechanism of e6-mediated p53 degradation at the cellular level remain to be clarified. this study therefore examined the role of cell localization and ubiquitination in the e6-mediated degradation of p53. as demonstrated within, following coexpression bo ...	2005	15994771
[the association of hela cell apoptosis induced by arsenic trioxide with the down-regulation of hpv18 e6 oncogene and inhibition of telomerase activity].	to study the effect of as2o3 induction of hela cell apoptosis on hpv18 e6 expression and telomerase activity.	2005	15996315
[assessment of human papilloma virus (hpv) testing in primary screening for cervical cancer in france (may 2004)].		2005	15999430
enlarged squamous cell nuclei in cervical cytologic specimens from perimenopausal women ("pm cells") : a cause of asc overdiagnosis.	we studied the appropriateness of interpreting squamous cells with enlarged, smooth, bland nuclei in perimenopausal women ("pm cells") as atypical squamous cells (ascs). papanicolaou smears (paps) from 100 women (40-55 years old) with a cytologic interpretation of asc of undetermined significance (ascus) and human papillomavirus (hpv) testing or a biopsy within 6 months were reviewed by 2 observers without knowledge of the biopsy diagnosis or hpv results. cases in which both reviewers agreed tha ...	2005	15923175
sexual behaviour and high risk human papillomavirus infections in japanese women.		2005	15923306
immunohistochemical demonstration of multiple hpv types in laryngeal squamous cell carcinoma.	the aim of this study was to determine the prevalence of human papillomaviruses (hpv) types 6, 11, 16, 18, 31, 33, 42, 51, 52, 56 and 58 in laryngeal squamous cell carcinoma specimens using immunohistochemical reactions and to correlate the presence of hpv with the clinical and pathological characteristics of these patients. tissue samples were collected from 40 patients with primary laryngeal squamous cell carcinoma (lscc) and from 33 subjects with non-neoplastic laryngeal lesions or laryngeal ...	2005	15924277
the human papillomavirus 16 e6 protein can either protect or further sensitize cells to tnf: effect of dose.	high-risk strains of human papillomavirus, including hpv 16, cause human cervical carcinomas, due in part to the activity of their e6 oncogene. e6 interacts with a number of cellular proteins involved in host-initiated apoptotic responses. paradoxically, literature reports show that e6 can both protect cells from and sensitize cells to tumor necrosis factor (tnf). to examine this apparent contradiction, e6 was transfected into u2os cells and stable clones were treated with tnf. intriguingly, clo ...	2005	15933739
human papillomavirus type 18 variant lineages in united states populations characterized by sequence analysis of lcr-e6, e2, and l1 regions.	while hpv 16 variant lineages have been well characterized, the knowledge about hpv 18 variants is limited. in this study, hpv 18 nucleotide variations in the e2 hinge region were characterized by sequence analysis in 47 control and 51 tumor specimens. fifty of these specimens were randomly selected for sequencing of an lcr-e6 segment and 20 samples representative of lcr-e6 and e2 sequence variants were examined across the l1 region. a total of 2770 nucleotides per hpv 18 variant genome were con ...	2005	15936050
outcome after a cervical cytology report of low-grade squamous abnormality in australia.	the current study was undertaken to assist with providing an evidence-based approach to the management of women with cervical cytology reports of low-grade squamous abnormality as used in the australian organized screening program.	2005	15937925
hpv 16 e6 sequence variations in indian patients with cervical neoplasia.	in a cross-sectional study performed between june 2001 and november 2003, the hpv 16 e6 gene of 50 women with cervical neoplasia and 20 cytologically normal women ('controls') was sequenced following amplification by pcr. the 350t to g variant was seen in 35 (70%) of 50 patients' isolates while it was seen in only 3 (15%) of the 20 isolates from the 'controls'. the higher occurrence of the 350g variant among the patients was statistically significant (p<0.01). isolates from patients were grouped ...	2005	15939534
[intraepithelial cervical squamous lesions: comparison between pap-test, colposcopy, phase contrast microscopy and histology; usefulness of their synergic use].	in the last 10 years the tumors of cervix have showed a significant reduction in incidence, while the preneoplastic lesions are increased (linked often to human papilloma virus [hpv] infection), and so it is enhanced the role of early diagnosis.	2005	15940075
acquisition and persistence of human papillomavirus infection in younger men: a prospective follow-up study among danish soldiers.	no data is yet available on incidence or persistence of human papillomavirus (hpv) infection in men. we enrolled 374 younger male conscripts (18-29 years) in a prospective study, and they were examined twice with an interval of 6 to 8 months. data collection included a questionnaire and a sample of cells from the penis for hpv detection using pcr. in addition, the presence of chlamydia trachomatis dna was assessed in urine samples by means of pcr. the hpv prevalence at the first and second exami ...	2005	15941967
extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells.	the ectopic expression of antibody fragments inside mammalian cells (intrabodies) is a challenging approach for probing and modulating target activities. we previously described the shuttling activity of intracellularly expressed escherichia coli beta-galactosidase conferred by the single-chain fv (scfv) fragment 13r4 equipped with nuclear import/export signals. here, by appending to scfvs the proteolytic pest signal sequence (a protein region rich in proline, glutamic acid, serine and threonine ...	2005	15943819
b lymphocyte activation by human papillomavirus-like particles directly induces ig class switch recombination via tlr4-myd88.	vaccination with human papillomavirus type 16 (hpv16) l1 virus-like particles (vlp) induces both high titer neutralizing igg and protective immunity. because protection from experimental infection by papillomavirus is mediated by neutralizing igg, we sought the mechanisms that trigger humoral immunity to hpv16 l1 vlp. we find that hpv16 l1 vlp bind to murine b lymphocytes thereby inducing activation-induced cytidine deaminase expression and ig class switch recombination to cause the generation o ...	2005	15944297
ultra-deformable liposomes containing bleomycin: in vitro stability and toxicity on human cutaneous keratinocyte cell lines.	formulations of ultra-deformable liposomes containing bleomycin (bleosome) have previously been described and proposed for topical treatment of skin cancer [lau, k.g., chopra, s., maitani, y., 2003. entrapment of bleomycin in ultra-deformable liposomes. s. t. p. pharm. sci. 13, 237-239]. in this study, the stability of various bleosome formulations was characterised and a purification process was established to isolate bleosome for testing on cultures of either human cutaneous keratinocytes (neb ...	2005	15946810
a single amino acid substitution improves the in vivo immunogenicity of the hpv16 oncoprotein e7(11-20) cytotoxic t lymphocyte epitope.	one of the few and most extensively studied human papilloma virus (hpv) type 16 oncoprotein e7-derived cytotoxic t lymphocyte (ctl) epitopes is ymldlqpett, presented to ctl by hla-a2.1. we previously identified an altered peptide ligand (apl) of this epitope with increased binding affinity for hla-a2.1, ymldlqpetv. herein, the in vivo immunogenicity of this apl was investigated in hla-a2.1 transgenic hhd mice. both in vitro and direct ex vivo analysis, performed using newly generated hhd tetrame ...	2005	15963358
diagnosis and management of oncogenic cervical human papillomavirus infection.	cervical hpv infection should be managed less as a typical sti and more as a strong risk factor predisposing to cervical cancer development. hpv infection is undeniably transmitted predominately through sexual contact.however, the fact that more than 80% of women followed over time will acquire at least one hr-hpv infection reflects the ubiquitous nature of the infection and the ease of transmission. although the behavioral profiles typically associated with an increased risk for sti (including ...	2005	15963882
multiple neoplastic lesions of the lower genital tract in a human papilloma virus-infected kidney-pancreas allograft recipient: a case report.	states of immunodeficiency are associated with an increased rate of certain cancers. immunosuppressed allograft recipients are at high risk of human papilloma virus (hpv)-related de novo malignancies. a female pancreas plus kidney transplant patient developed multiple genital malignancies within 6 years. the genome of human papilloma virus type 16 was detected in malignant lesions obtained from surgical procedures. all detected lesions were removed at an early state of development.	2005	15964348
[modulator role of p16ink4a in human papillomavirus infection].		2005	15970162
the impact of high risk human papillomavirus testing in an inner london colposcopy clinic.	this is an audit of a new technique to improve the colposcopy service. samples were tested for high risk hpv dna using digene hybrid capture ii. sixty-four percent of the sampled women under 30 had detectable high risk hpv dna, decreasing to 44% in 30--39 year olds and to 27% in women over 40. high risk hpv prevalence increased with severity of cytology, although 22% with normal colposcopy had detectable high risk hpv. of those women treated for cervical dysplasia, 83% had detectable high risk h ...	2005	15977240
technology evaluation: hspe7 (stressgen).	stressgen is developing hspe7, a recombinant fusion protein comprising the human papillomavirus (hpv) e7 antigen and the heat shock protein hsp65 from mycobacterium bovis, as a potential therapy for conditions associated with hpv infection. this therapy is currently undergoing phase iii clinical trials.	2005	15977424
financial requirements of immunisation programmes in developing countries: a 2004-2014 perspective.	vaccines are a key contributor to public health, especially in developing countries. despite numerous demonstrations of the cost-effectiveness of immunisation, vaccines spending accounted for only 1.7% of the total pharmaceutical market in 2002, when unicef estimated that 34 million children were not reached by routine immunisation, most of them in developing countries. several international organizations or initiatives, like the global alliance for vaccines and immunisation (gavi), have defined ...	2005	15979769
prostate cancer and sexually transmitted diseases: a meta-analysis.	prostate cancer is the most common neoplasm of american men and the second most common cause of cancer-related deaths. research suggests that infection and subsequent inflammation may be an important risk factor in the pathogenesis of prostate cancer. in this meta-analysis, we examine the current epidemiological evidence for the association between specific sexually transmitted diseases (stds) and prostate cancer.	2005	15988645
the role of viral integration in the development of cervical cancer.	the development of invasive cervical cancer is associated with human papillomavirus (hpv) infection and subsequent integration into the host epithelium. more than 99% of cervical cancers contain hpv sequences, and many of these contain a truncated hpv genome integrated into a single position within the host genome. studies examining the role of viral integration in cervical cancer development have found that the sites of integration appear randomly distributed throughout the genome. this, and th ...	2005	15771901
1h and 15n resonance assignment, secondary structure and dynamic behaviour of the c-terminal domain of human papillomavirus oncoprotein e6.	e6 is a viral oncoprotein implicated in cervical cancers, produced by human papillomaviruses (hpvs). e6 contains two putative zinc-binding domains of about 75 residues each. the difficulty in producing recombinant e6 has long hindered the obtention of structural data. recently, we described the expression and purification of e6-c 4c/4s, a stable, folded mutant of the c-terminal domain of hpv16 e6. here, we have produced 15n-labelled samples of e6-c 4c/4s for structural studies by nmr. we have as ...	2005	15772752
absence of human papillomavirus in esophageal carcinomas from southwestern kenya.	esophageal squamous cell cancer is highly prevalent in south-western kenya. the role of human papillomavirus (hpv) in esophageal cancers from this region was evaluated. biopsies of 29 esophageal squamous cell cancers were assayed for hpv dna sequences by reverse line blot polymerase chain reaction, using 27 hpv type-specific probes. viral sequences were found in none of the specimens. these results suggest the hpv is unlikely to be an etiologic factor for esophageal squamous cell cancers in this ...	2005	15773838
human papillomavirus genotypes and their association with cervical neoplasia in a cohort of western australian women.	human papillomavirus (hpv) is known to be the cause of almost all cervical cancers. the genotypes have been classified into high and low risk types according to their oncogenic potential. however, data for many of the genotypes are limited and some (hpv-26, 53, and 66) have no agreed status. a study was undertaken to determine the hpv genotype distribution in women of western australia and the association with cervical neoplasia. liquid based cervical samples from a cohort of 282 western austral ...	2005	15778980
after the promise: the std consequences of adolescent virginity pledges.	to examine the effectiveness of virginity pledges in reducing std infection rates among young adults (ages 18-24).	2005	15780782
detection of persistent high risk human papillomavirus infections with hybrid capture ii and spf10/lipa.	hpv infection in young women is common. however only a certain number of hpv genotypes are oncogenic. it is necessary for high risk hpv infection to persist at the cervix for a considerable time before oncogenesis occurs.	2005	15780805
opposing effects of bovine papillomavirus type 1 e6 and e7 genes on fas-mediated apoptosis.	programmed cell death (pcd), best exemplified by apoptosis, is a genetically programmed process of cellular destruction that is indispensable for normal development and homeostasis of multicellular organisms. tumor necrosis factor alpha (tnf) and related cytokines are employed by host defenses to eliminate virally infected cells through induction of apoptosis. many viruses have evolved specific gene products to modulate this process. we have recently shown that the bovine papillomavirus type 1 ( ...	2005	15782122
immunotherapeutic efficacy of vaccines generated by fusion of dendritic cells and hpv16-associated tumour cells.	utilization of vaccines generated by fusion of dendritic cells and tumour cells is a promising approach to tumour immunotherapy. we have examined the therapeutic efficacy of vaccines generated by fusion of hpv16-associated tumour cells tc-1 with syngeneic and allogeneic dendritic cells. locally administered hybrid cells generated by fusion of mhc class i+ tc-1 cells and syngeneic dc inhibited the growth of mhc class i+ tc-1 tumours, but not the growth of mhc class i- tc-1/a9-derived tumours. the ...	2005	15783088
incidence of cervical squamous intraepithelial lesions associated with hiv serostatus, cd4 cell counts, and human papillomavirus test results.	recent cervical cancer screening guidelines state that the interval between screenings can be safely extended to 3 years in healthy women 30 years or older who have normal cytology results and have negative test results for oncogenic human papillomavirus (hpv) dna.	2005	15784870
is p16(ink4a) expression more useful than human papillomavirus test to determine the outcome of atypical squamous cells of undetermined significance-categorized pap smear? a comparative analysis using abnormal cervical smears with follow-up biopsies.	to correlate high-risk human papillomavirus (hr-hpv) viral load to p16(ink4a) expression in atypical squamous cells of undetermined significance (ascus)-categorized pap smears with follow-up biopsies in order to elucidate their relationships in gynecological pathology.	2005	15790434
vascular endothelial growth factor (vegf) up-regulates epidermal growth factor receptor (egf-r) in cervical cancer in vitro: this action is mediated through hpv-e6 in hpv-positive cancers.	epidermal growth factor receptor (egf-r) up-regulation in cervical cancer cells leads to an increase in cell proliferative insulin-like growth factor ii (igf-ii) and vascular endothelial growth factor (vegf) and a decrease of the anti-proliferative igf-binding protein-3 (igf-bp3). the objectives for this study are: (a) to find if vegf, in turn, up-regulates egf-r and down-regulates igf-bp3; (b) to determine if human papilloma virus (hpv-e6) mediates this action of vegf in hpv-positive cells; and ...	2005	15790460
parallel nanoliter detection of cancer markers using polymer microchips.	a general multipurpose microchip technology platform for point-of-care diagnostics has been developed. real-time nucleic acid sequence-based amplification (nasba) for detection of artificial human papilloma virus (hpv) 16 sequences and siha cell line samples was successfully performed in cyclic olefin copolymer (coc) microchips, incorporating supply channels and parallel reaction channels. samples were distributed into 10 parallel reaction channels, and signals were simultaneously detected in 80 ...	2005	15791339
antiviral activity of cidofovir on a naturally human papillomavirus-16 infected squamous cell carcinoma of the head and neck (scchn) cell line improves radiation sensitivity.	high risk hpv types 16 and 18 are associated with cervical cancer and squamous cell carcinoma of the head and neck (scchn). cidofovir is an antiviral drug used to treat hpv-induced laryngeal papillomatosis and other viral infections, with initial reports suggesting activity in cervical carcinoma cells. we investigated the effects of cidofovir on a naturally hpv-16-transformed scchn cell line (upci:scc090), in comparison with a cervical carcinoma cell line (caski) of similar viral characteristics ...	2005	15792615
the mitotic chromosome binding activity of the papillomavirus e2 protein correlates with interaction with the cellular chromosomal protein, brd4.	the papillomavirus transcriptional activator, e2, is involved in key functions of the viral life cycle. these include transcriptional regulation, viral dna replication, and viral genome segregation. the transactivation domain of e2 is required for each of these functions. to identify the regions of the domain that mediate binding to mitotic chromosomes, a panel of mutations has been generated and their effect on various e2 functions has been analyzed. a structural model of the bovine papillomavi ...	2005	15795266
induction of the human papillomavirus type 31 late promoter requires differentiation but not dna amplification.	the human papillomavirus (hpv) life cycle is linked to the differentiation state of the host cell. in virus-infected undifferentiated basal epithelial cells, hpv genomes are maintained as episomes at low copy number. upon differentiation, a concomitant increase in viral copy number and an induction of late gene expression from a differentiation-specific promoter is seen. to investigate whether late gene expression was dependent on the amplification of the viral genome, inhibitors of dna replicat ...	2005	15795277
adenoviral p53 effects and cell-specific e7 protein-protein interactions of human cervical cancer cells.	we investigated the time-course tumor growth suppression effects of recombinant adenovirus expressing p53 on human cervical cancer cells and cell-specific e7 protein-protein interactions in cell lysates using surface plasmon resonance (spr) biosensor. six hpv-infected human cervical cancer cell lines (hpv 16-positive cells, caski and siha cells; hpv 18-positive cells, hela and helas3 cells; and hpv negative c33a and ht3 cells) were used. after infection with adcmvp53, the cell-specific growth in ...	2005	15797321
immunohistochemical expression of p16ink4a and bcl-2 according to hpv type and to the progression of cervical squamous intraepithelial lesions.	inactivation of the cell cycle inhibitor gene p16mts1 seems to be involved in human papillomavirus (hpv)-related carcinogenesis because e6 and e7 oncoproteins may impair p16ink4a and, indirectly, bcl-2 functions. in this study, we analyzed the role of immunohistochemical expression of p16ink4a and bcl-2 in hpv-infected cervical biopsies as prognostic markers of the progression of squamous intraepithelial lesion (sil). sixty-five cervical biopsies were stratified into two subgroups according to t ...	2005	15805425
hpv type concordance in sexual couples determines the effect of condoms on regression of flat penile lesions.	we earlier demonstrated, in a randomised clinical trial, that the regression time of flat penile lesions in male sexual partners of women with cervical intraepithelial neoplasia (cin) was shorter in men who used condoms compared to those who did not. to further evaluate this finding, we examined whether the effect of condom use on the regression of flat penile lesions depends on the presence of human papillomavirus (hpv) type concordance in sexual couples, as determined in cervical and penile sc ...	2005	15812547
inhibitory effect of jaceosidin isolated from artemisiaargyi on the function of e6 and e7 oncoproteins of hpv 16.	jaceosidin (4',5,7-trihydroxy-3',6-dimethoxyflavone) was isolated from artemisia argyi as a putative oncogene inhibitor. jaceosidin inhibited binding between oncoprotein e6 of the human papillomavirus and the p53 tumor suppressor protein. in addition, jaceosidin inhibited binding between the e7 oncoprotein and the rb tumor suppressor protein, and also inhibited the function of hpv-16 harboring cervical cancer cells, including siha and caski. collectively, jaceosidin inhibited the functions of th ...	2005	15814270
family history as a co-factor for adenocarcinoma and squamous cell carcinoma of the uterine cervix: results from two studies conducted in costa rica and the united states.	previous work suggests that cervical cancer may aggregate in families. we evaluated the association between a family history of gynecological tumors and risk of squamous cell and adenocarcinomas of the cervix in 2 studies conducted in costa rica and the united states. the costa rican study consisted of 2,073 women (85 diagnosed with cin3 or cancer, 55 diagnosed with cin2 and 1,933 controls) selected from a population-based study of 10,049 women. the u.s. study consisted of 570 women (124 with in ...	2005	15818615
induction of human papillomavirus type 16-specific immunologic responses in a normal and an human papillomavirus-infected populations.	human papillomavirus (hpv) infection, especially with the oncogenic genotypes, is the most important risk factor for developing cervical cancer. we focused on generating hpv16 e7-specific cytotoxic cd8(+) t lymphocytes and evaluating hpv16 e7-specific immune responses in hpv16-infected and uninfected populations. peripheral blood mononuclear cells (pbmcs) were first collected from an uninfected group with an human lymphocyte antigen (hla) a2 haplotype (four volunteers). mature monocyte-derived d ...	2005	15819706
human papillomavirus genome detection by in situ hybridization in fine-needle aspirates of metastatic lesions from head and neck squamous cell carcinomas.	patients with head and neck squamous cell carcinoma (hnscc) often present with metastatic disease. the diagnosis of metastatic lesions usually is determined by fine-needle aspiration. human papillomavirus (hpv) is now being considered as a causative agent in a subset of hnscc. the objectives of this study were, first; to search for the presence of hpv dna by in situ hybridization (ish) in metastatic lesions from hnscc using alcohol-fixed, archival, cytopathologic material; second, to characteriz ...	2005	15822131
human papillomavirus status in advanced cervical cancer: predictive and prognostic significance for curative radiation treatment.	human papillomavirus (hpv) infection plays a major role in oncogenesis of squamous cell carcinoma of the cervix. this study was performed to investigate if hpv status and e2 gene integrity are prognostic parameters for clinical outcome and predictive for radiation response. forty women with locally advanced cervical cancer treated with curative radiotherapy were analyzed for hpv infection and e2 gene integrity by multiplex polymerase chain reaction. statistical analyses were performed for overal ...	2005	15823112
a case with multiple gynecological malignancies.	a patient with cervical non-hodgkin lymphoma was treated with chemotherapy. fourteen months after the diagnosis of the lymphoma, an endometrial adenocarcinoma was detected as a secondary malignant tumor. the patient was treated with surgery followed by radiotherapy. approximately 7 years after the diagnosis of endometrial cancer, vaginal invasive squamous cell carcinoma was diagnosed as the third primary malignancy, and a second-line palliative radiotherapy was applied. seven months after the la ...	2005	15823128
human papillomaviruses in lymph node neck metastases of head and neck cancers.	the results of this study corroborate earlier findings that human papillomavirus (hpv)16 is the most prevalent type of hpv in squamous cell carcinomas of the head and neck (scchns) and reinforce a possible influence of hpv on scchn progression by showing that the majority of hpv-positive patients harbor hpv16 (or hpv33) both in their primary tumors and in lymph node neck metastases (lnnms).	2005	15823814
cell-surface display of e7 antigen from human papillomavirus type-16 in lactococcus lactis and in lactobacillus plantarum using a new cell-wall anchor from lactobacilli.	the human papillomavirus type-16 (hpv-16) e7 protein is considered a major viral oncoprotein involved in cervical cancer (cxca) and a potential candidate for the development of a vaccine against this neoplasia. here, two lactic acid bacteria (the model one lactococcus lactis and a probiotic one lactobacillus plantarum) were engineered to deliver an e7 mutant protein (e7mm), which has a reduced transforming activity and consequently, could fit better to therapeutic use in humans than the native f ...	2005	15823960
human papillomavirus 16 virus-like particles use heparan sulfates to bind dendritic cells and colocalize with langerin in langerhans cells.	langerhans cells (lc), the immature dendritic cells (dc) that reside in epithelial tissues are among the first immune cells to encounter human papillomavirus (hpv) and are not activated by hpv virus-like particles (vlps) in contrast to dc. the notion that the differences in response to hpv vlps between lc and dc are associated with different types of cell binding and intracellular trafficking has been addressed. inhibition experiments with heparin and sodium chlorate showed that heparan sulfates ...	2005	15831940
proteomic analysis of progressive factors in uterine cervical cancer.	human papillomavirus (hpv) infections play a crucial role in the progress of cervical cancer. the high-risk hpv types are frequently associated with the development of malignant lesions. some of the latest studies have demonstrated that the high-risk hpv 16 and 18 are predominantly detected in the more aggressive cancers. in the present study, we aimed to establish the proteomic profiles and characterization of the tumor related proteins by using two-dimensional gel electrophoresis (2-de) and ma ...	2005	15838902
genital human papillomavirus testing by in situ hybridization in liquid atypical cytologic materials and follow-up biopsies.	to describe cases of hpv testing by dna in situ hybridization performed on atypical cervicovaginal samples collected by a liquidsed method that were negative for hpv dna on cytology but revealed cervical intraepithelial neoplasia on follow-up biopsies.	2005	15839614
study of the viral infections and cytokines associated with recurrent aphthous ulceration.	mouth ulcers are one of the most common oral complaints. however, the association between oral ulceration and viruses and cytokines is uncertain. we detected the presence of human papilloma virus (hpv), cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv)-1, hsv-2 and human herpesvirus (hhv)-8 dna in oral tissues by polymerase chain reaction (pcr) and southern hybridization techniques, and quantified the serum levels of cytokines including interleukin (il)-2, il-4, granulo ...	2005	15840465