Publications

enzymatic conversion of adenosine to inosine in the wobble position of yeast trnaasp: the dependence on the anticodon sequence.	we have investigated the specificity of the trna modifying enzyme that transforms the adenosine at position 34 (wobble position) into inosine in the anticodon of several trnas. for this purpose, we have constructed sixteen recombinants of yeast trnaasp harboring an axy anticodon (where x or y was one of the four nucleotides a, g, c or u). this was done by enzymatic manipulations in vitro of the yeast trnaasp, involving specific hydrolysis with s1-nuclease and rnaase a, phosphorylation with t4-po ...	1984	6369251
theoretical consideration of the effects of dilution on estimates of free thyroid hormones in serum.	when concentrations of free thyroid hormones in serum are measured by equilibrium dialysis, the serum sample is diluted by dialysis buffer, resulting in a new equilibrium for distribution of these hormones that differs from that in the original serum sample. we have derived a mathematical formulation, based on an equilibrium model for multiple ligands and multiple binding sites, to determine the effect of dilution on the concentrations of free hormones in serum. computer simulations based on the ...	1984	6370496
sequence and cloning of bacteriophage t4 gene 63 encoding rna ligase and tail fibre attachment activities.	the sequence of gene 63 of bacteriophage t4 was determined by a shotgun approach. small dna fragments, derived by sonication of a restriction fragment that encompasses the region of gene 63, were cloned in m13 vectors and sequenced by the 'dideoxy' method. the position of the gene was established by comparison with the sequence of a gene 63 amber mutant. knowledge of the dna sequence of gene 63 and surrounding regions has allowed the construction of a clone of gene 63 in which rna ligase product ...	1984	6370680
behçet's syndrome: immune regulation, circulating immune complexes, neutrophil migration, and colchicine therapy.	immune regulatory dysfunction, circulating immune complexes (cic), and polymorphonuclear (pmn) cell migration were investigated in patients with behçet's syndrome. six patients meeting rigorous clinical criteria were evaluated. only one patient showed evidence of immune regulatory dysfunction (increased t4/t8 ratio). although c1q binding and raji cell assays for cic yielded positive results in only one of five patients, all five patients had in vivo "histamine trap test" evidence of cic (all con ...	1984	6371066
phenotypic suppression of opal mutants by l-tryptophan.	l-tryptophan (codon ugg) at high concentrations, usually 20 mm, phenotypically suppressed all four phage t4 opal (codon uga) mutants tested. the suppression was incomplete. with three of the opal mutants, h301, h317, and h340, the suppression resulted in marked increases in plaque size and clarity in the restrictive host escherichia coli strain b. with the very tight opal mutant h429, the suppression was discerned only by a 1,200-fold increase in phage yield from restrictive host cells in the ch ...	1984	6371450
[changes in the state of tryptophan residues in t4 phage lysozyme during binding to a competitive inhibitor].	the analysis of the intrinsic fluorescence parameters of t4 phage lysozyme in free state and in complex with inhibitor--disaccharide-tetrapeptide from the e. coli cell wall has been carried out. a comparison of the fluorescence changes with the results obtained by difference spectrophotometry and with the data of elwell and schellman on the intrinsic fluorescence of wild type wt and mutant eri t4 phage lysozymes and a consideration of the three dimensional structure of the protein allows to repr ...	1984	6371490
the role of thyroid hormone deiodination in the regulation of hypothalamo-pituitary function.	there is extensive deiodinative metabolism of thyroxine (t4) in thyroid hormone target organs, including the pituitary and brain. in both rat and man, most of the 3,3',5-triiodothyronine (t3) in the body is produced outside the thyroid gland by deiodination of t4. t3 is the principal active form of thyroid hormone within cells. in the rat, there are at least three enzymatic iodothyronine-deiodinating pathways which can be distinguished by kinetics and substrate and inhibitor specificities. two o ...	1984	6371572
euthyroid sick syndrome. association with urosepsis in an elderly man.	a 91-year-old man with chronic incontinence managed by indwelling catheter was hospitalized for investigation of fever, hypotension, and cloudy urine. dopamine was administered to maintain adequate blood pressure, and cefazolin and tobramycin were given for presumed urosepsis. persistent bradycardia suggested hypothyroidism, but laboratory data were consistent with euthyroid sick syndrome. thyroid values returned to normal with correction of the sepsis and improvement in nutrition. exogenous thy ...	1984	6371753
t4 rna ligase mediated preparation of novel "chemically misacylated" trnaphes.	t4 rna ligase was employed for the condensation of escherichia coli trnaphe missing cytidine-75 and adenosine-76 (trnaphe-coh; the acceptor "oligomer") with each of several chemically acylated derivatives of pcpa (the donor "oligomer"). the resulting "chemically misacylated " trnaphes were obtained in 20-65% yields following chromatographic workup on deae-cellulose and benzoylated deae-cellulose. characterization of the chemically misacylated trnas was accomplished by (i) enzymatic reaminoacylat ...	1984	6372858
t lymphocyte repopulation and differentiation after bone marrow transplantation. early shifts in the ratio between t4+ and t8+ t lymphocytes correlate with the occurrence of acute graft-versus-host disease.	acute graft-versus-host disease (gvhd), a major complication of allogeneic bone marrow transplantation (bmt), is probably mediated by t lymphocytes present in the marrow graft. in this study, the repopulation of the peripheral blood with t4+ and t8+ t cells was investigated during the period preceding the occurrence of acute gvhd. twenty-four allogeneic and 11 autologous bmt recipients were monitored from day 4 post-bmt onward by the use of monoclonal antibodies, indirect immunofluorescence, and ...	1984	6372896
two-step resistance by escherichia coli b to bacteriophage t2.	numerous authors have noted the difficulty in obtaining mutants of e. coli b that are resistant to bacteriophage t2 using standard procedures of plating large numbers of cells in the presence of excess phage. yet, t2-resistant mutants appear in continuous culture at rates in consistent with this difficulty. this paradoxical result derives from the fact that resistance to t2 usually arises as a consequence of two nonindependent mutations. mutant bacteria resistant to phage t4 are very common and ...	1984	6373494
glucose metabolism in experimental hyperthyroidism: intact in vivo sensitivity to insulin with abnormal binding and increased glucose turnover.	the characteristics of the dose response of insulin on the glucose turnover rate and erythrocyte insulin binding parameters were determined in five normal men before and during experimentally induced hyperthyroidism [l-t4 (2 micrograms kg-1 day-1) for 4 weeks with additional l-t3 (1 microgram kg-1 day-1) for the following 3 weeks]. hyperthyroidism was characterized by significant rises in t3 from 1.92 +/- 0.17 (+/- sem) to 3.66 +/- 0.17 nmol/liter (p less than 0.01) and resting metabolic rate fr ...	1984	6373813
altered blood t-cell subsets in patients with multiple sclerosis.	we have found an alteration in t-cell subsets in patients with active multiple sclerosis, specifically an increase in the t4:t8 ratio. these findings have been reproducibly obtained over the past four years, occurring in the majority of acute patients tested early in the course of an attack and in between 25 and 40% of chronic progressive patients, depending on their stage of illness. these changes correlate with pleocytosis in spinal fluid and with other abnormalities of immune function, such a ...	1984	6373819
immunopathology of pityriasis lichenoides acuta.	eleven biopsy specimens (five papules and six dusky or crusted lesions) from four patients with pityriasis lichenoides et varioliformis acuta ( pleva ) were studied by direct immunofluorescence and immunoperoxidase technics. slight vascular deposits of igm and c3 were present in most lesions. slight perivascular deposits of fibrin were observed in early lesions; more extensive perivascular and interstitial deposits of fibrin were detected in advanced lesions. most of the infiltrating cells were ...	1984	6373855
phenotypic characterization of human t lymphocyte populations producing macrophage-activating factor (maf) lymphokines.	human t lymphocytes, which are known to produce lymphokines that can activate monocytes and macrophages, can be subdivided into two functionally relevant subsets, as indicated by the expression of the t4 and t8 antigens. to determine whether one or both of these t cell subsets is capable of generating macrophage activating factor(s) (maf) after stimulation with lectin, soluble antigen, or cell surface alloantigen, highly purified populations of t4 or t8 cells were cultured in medium containing p ...	1984	6373929
valyl-trna synthetase modification-dependent restriction of bacteriophage t4.	a strain of escherichia coli, cp 790302, severely restricts the growth of wild-type bacteriophage t4. in broth culture, most infections of single cells are abortive, although a few infected cells exhibit reduced burst sizes. in contrast, bacteriophage t4 mutants impaired in the ability to modify valyl-trna synthetase develop normally on this strain. biochemical evidence indicates that the phage-modified valyl-trna synthetase in cp 790302 is different from that previously described. although the ...	1984	6374167
[effect of thyroxine in the g0 stage of the cell cycle on the yield of chromosome aberrations in rat hepatocytes and human lymphocytes after x-irradiation].	a study was made of the mechanism of a modifying action of thyroxin (t4) on the chromosome integrity after the application thereof in vivo and in vitro following a single whole-body exposure to x-radiation with a dose of 2.19 gy. it is concluded that t4 influences the quantity of cells with chromosome aberrations stimulating the recovery of the chromosome integrity: the stimulation can be brought about at the g0 stage of the cell cycle as a result of the direct effect of the hormone on a cell.	1984	6374746
[cellular immunology tests applied to the diagnosis of immune system dysfunctions].	in clinical immunology, improved standardisation and simplification have made skin and laboratory tests more amenable both to routine use and more rigorous interpretation. an individual's immune status can be assessed by the in vivo investigation of delayed hypersensitivity to widely distributed antigens or by techniques such as the mitogen test ot lymphoblast transformation, lymphocyte population and sub-population (e.g. t4, t8...) counts. a more delicate test of phagocytosis is provided by the ...	1984	6375474
lymphocytes in the skin of patients with progressive systemic sclerosis. quantification, subtyping, and clinical correlations.	mononuclear cells (mnc) present in the dermis of the forearm and in the blood of patients with progressive systemic sclerosis (pss) were quantified and analyzed for subsets using monoclonal antibodies. the findings were correlated with cutaneous and systemic features of the disease. total t lymphocytes and their subsets, b cells, and macrophages were enumerated in the skin samples of 21 patients with pss. the dermal mnc infiltrates consisted mostly of activated t lymphocytes with a mean t helper ...	1984	6375682
effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator t4 dna polymerase mutant.	the effect of temperature on genetically well-defined mutational pathways was examined in the bacteriophage t4. the mutational site was a t4 rii ochre mutant which could revert to rii+ via a transversion or to the amber convertant via a transition. temperature did not strongly affect any of the pathways examined in a wild-type background; however, increased temperature reduced the mutational activity of a mutator dna polymerase mutant. possible models to explain the role of temperature in mutage ...	1984	6375839
base-pairing properties of o6-methylguanine in template dna during in vitro dna replication.	the kinetics of incorporation of deoxynucleotide precursors directed by the promutagenic base, o6-methylguanine (m6gua), was analyzed during in vitro replication of m6gua-containing synthetic polydeoxynucleotides by t4 and t5 phage dna polymerases and escherichia coli dna polymerase i. when poly(dt,m6dg) and poly(dc,m6dg) with covalently attached primers were replicated, o6-methylguanine paired with either thymine or cytosine but with a much higher preference for thymine. dctp and dttp acted as ...	1984	6376499
heterogeneity of mammalian dna ligase detected on activity and dna sequencing gels.	a new method to detect dna ligase activity in situ after nadodso4 polyacrylamide gel electrophoresis has been developed. after renaturation of active polypeptides the ligase reaction occurs in situ by incubating the intact gel in the presence of mg++ and atp. further treatment with alkaline phosphatase removes the unligated 5'-32p-end of oligo (dt) used as a substrate and active polypeptides having ligase activity are identified by autoradiography. analysis on dna sequencing gels of the oligo (d ...	1984	6377238
selective affinity chromatography of dna polymerases with associated 3' to 5' exonuclease activities.	the use of 5'-amp as a ligand for the affinity chromatography of dna polymerases with intrinsic 3' to 5' exonuclease activities was investigated. the basis for this is that 5'-amp would be expected to act as a ligand for the associated 3' to 5' exonuclease. the requirements for binding of escherichia coli dna polymerase i, t4 dna polymerase, and calf thymus dna polymerase delta, all of which have associated 3' to 5' exonuclease activities, to several commercially available 5'-amp supports with d ...	1984	6377960
conformational and functional similarities between glutaredoxin and thioredoxins.	the tertiary structures of thioredoxin from escherichia coli and bacteriophage t4 have been compared and aligned giving a common fold of 68 c alpha atoms with a root mean square difference of 2.6 a. the amino acid sequence of glutaredoxin has been aligned to those of the thioredoxins assuming that glutaredoxin has the same common fold. a model of the glutaredoxin molecule was built on a vector display using this alignment and the t4 thioredoxin tertiary structure. by comparison of the model with ...	1984	6378624
bacteriophage t4 bypass31 mutations that make gene 31 nonessential for bacteriophage t4 replication: mapping bypass31 mutations by uv rescue experiments.	the product of gene 31 is normally required for assembly of the t4 capsid. two mutations that each bypass that requirement are shown to be located at separate sites in gene 23, which encodes the major structural protein of the capsid. a second phenotypic effect that characterizes both bypass31 mutant strains is the ability to multiply in host-defective strains, such as hdb3-1 and groel mutants, on which wild-type t4 is unable to assemble capsids. the genetic data indicate that both phenotypic ef ...	1984	6379205
bacteriophage t4 bypass31 mutations that make gene 31 nonessential for bacteriophage t4 replication: isolation and characterization.	t4 bacteriophage mutants called bypass31 (byp31) that specifically suppress gene 31 amber mutations have been isolated and characterized. the mechanism by which the byp31 mutation, byp31-1, suppresses gene 31 nonsense mutations does not involve synthesis of gp31 or of a particular gp31 fragment; furthermore, the byp31 allele suppresses all nonsense mutations in gene 31 that have been tested. we detect no unusual properties among the t4 particles made in su- cells by the t4amn54byp31-1 double mut ...	1984	6379206
[a case of spinal epidural abscess in a renal transplant recipient].	a 44-year-old male underwent a cadaveric renal transplantation at the university hospital of tsukuba on march 3, 1979. on june 11, 1979, he was discharged this hospital. immunosuppressive therapy with azathioprine and methylprednisolone was done. on november 1, 1979, he developed back pain associated with fever. on november 3, 1979, he was admitted to the university hospital of tsukuba. laboratory study revealed leucocytosis. crp inserum was positive. but pneumonia, urinary tract infection and i ...	1984	6379492
[verification of the effectiveness of neutralization in the in vivo study of antiseptics].	well defined controls of the effectiveness of antiseptic neutralizing agents in vitro already exist (afnor standards for antiseptics and disinfectants). conversely, there is currently no standard method for verifying how effectively such agents neutralize antiseptics which have been applied to the skin. we describe a method in which the neutralizing solution is first applied to the antiseptic-treated skin and then filtered to remove skin flora. the effect of the neutralizing agent is then checke ...	1984	6379573
immunohistochemical identification of inflammatory cells in secretory and chronic otitis media and cholesteatoma using monoclonal antibodies.	monoclonal antibodies (okt 3,4,6,8 and okia) were used in conjunction with the avidin-biotin-peroxidase complex method to classify inflammatory cells in 6 biopsies of the middle ear mucosa in patients with secretory otitis media (som) and in 19 middle ear or mastoidal biopsies in patients with chronic otitis media (com). mononuclear cell infiltrates under the mucosal epithelium were found to consist mainly of t4 positive (helper-inducer) t-lymphocytes (50-60%). t8 positive (suppressor-cytotoxic) ...	1984	6380206
cleavage of trna precursors by the rna subunit of e. coli ribonuclease p (m1 rna) is influenced by 3'-proximal cca in the substrates.	trna precursor molecules that contain the cca sequence found at the 3' termini of all mature trnas are cleaved in vitro more readily by m1 rna, the catalytic subunit of e. coli rnaase p, than precursors that lack this sequence. the sensitivity to the cca sequence is not apparent when precursors are cleaved by the reconstituted rnaase p holoenzyme that contains both m1 rna and the protein subunit. these results have been obtained with monomeric precursor molecules encoded by the e. coli and human ...	1984	6380759
hybrid transfer rna genes in phage t4.	we describe the isolation and characterization of two unusual amber suppressor forms of t4 trnaleu. the sequences of the suppressor trnas can be described as hybrids of wild-type trnaleu and suppressor trnagln molecules: the chain lengths and majority of the nucleotide residues corresponded to trnaleu, but cua anticodons flanked by 2-14 residues were identical to trnagln. the uncertainty as to the exact number of flanking residues correlated with trnagln is due to the similarity of the two trna ...	1984	6380760
serum hormones and metabolites in fetally decapitated pigs.	the effects of fetal decapitation on serum hormones and metabolites were studied in utero in the pig. pig fetuses were decapitated at 45 days of gestation and serum sampled from the umbilical vein and artery of each fetus and from the uterine artery at 110 days of gestation. serum levels of cortisol were reduced in decapitated fetuses when compared to intact controls. the data suggest that the decapitated fetus derived its cortisol primarily from maternal sources. decapitation produced a deficie ...	1984	6381242
histiocytosis x cells and langerhans cells: enzyme histochemical and immunologic similarities.	the proliferating cell in histiocytosis x has been thought to be an abnormal langerhans cell since the identification in 1965 of the characteristic x-bodies morphologically identical to the birbeck granules of epidermal langerhans cells. this conclusion is based primarily on this finding and on morphologic similarities at the light microscopic level. enzyme histochemical data have been somewhat conflicting and have not provided strong support for this conclusion. recently, immunohistochemical st ...	1984	6381283
variations of thyroid hormones during total fasting in obese and in obese diabetic subjects.	fasting and hypocaloric diets are known to induce a reduction of triiodothyronine (t3) and to increase reverse triiodothyronine (rt3) in normal and obese subjects. the effect of 8-day fasting was evaluated on t3, thyroxine (t4), free t4, rt3, tsh, immunoreactive insulin (iri), thyroxine binding globulin (tbg) and glycemia in 21 obese subjects (5 males, 16 females) grouped according to the average starting blood glucose concentration in: group i, diabetic obese subjects (9 patients); group ii, no ...	1984	6381583
defining a bacteriophage t4 late promoter: bacteriophage t4 gene 55 protein suffices for directing late promoter recognition.	the rna polymerase from bacteriophage t4-infected escherichia coli, which specifically initiates transcription at phage t4 late promoters, is extensively modified by adp-ribosylation of core subunits and by binding several virus-encoded subunits. we show here that one of these subunits, the phage t4 gene 55 protein, designated gp55, alone endows unmodified rna polymerase core enzyme from uninfected e. coli with the ability to selectively initiate transcription at the phage t4 late promoters, wit ...	1984	6382259
use of monoclonal antibodies to quantitate t lymphocyte subpopulations in human cardiac allografts.	t cell subsets have been quantitated in 40 human cardiac biopsies to characterize the surface phenotype of t lymphocytes involved in acute rejection. seventeen biopsies were from patients receiving conventional immunosuppression, and 18 were from patients receiving cyclosporine (cys) as the major immunosuppressive drug. five biopsies were from nontransplanted hearts. frozen sections were treated with monoclonal antibodies of the leu series and an immunoperoxidase technique to determine numbers o ...	1984	6382712
intracellular morphogenesis of bacteriophage t4. i. gene dosage effects on early functions and tail fiber assembly.	mixed infection of nonpermissive bacteria by amber mutant and wild-type t4 phage reduces the burst size in a manner that depends on the nature of the gene product eliminated by the mutation. while the genotypic proportions produced by such mixedly infected cells were directly proportional to those in the starting mixtures, the fraction of the burst size compared to wild-type-infected cells was dependent on the kind of mutant used. for example, mutants in genes 56, 42, and 43, which control catal ...	1984	6382790
intracellular morphogenesis of bacteriophage t4. ii. head morphogenesis.	the relative phage yields of cells of escherichia coli infected with both wild-type and amber mutant phages deficient in head morphogenesis were determined. the decrease in burst size as a function of the ratio of mutant:wild-type-infecting phage was linear and proportional for mutants in genes 20, 22, and 23, while for mutants in genes 21, 31, and 24 the results suggest an excess of intracellular gene product. the initiation of assembly of phage particles was not delayed at reduced gene product ...	1984	6382791
monoclonal antibody analysis of mononuclear cells in myopathies. i: quantitation of subsets according to diagnosis and sites of accumulation and demonstration and counts of muscle fibers invaded by t cells.	in 76 muscle specimens (normal controls, 9; duchenne dystrophy, 11; scleroderma, 11; dermatomyositis, 13; polymyositis, 15; inclusion body myositis, 17), mononuclear cells were analyzed at perivascular, perimysial, and endomysial sites of accumulation. monoclonal antibodies reactive for b cells, t cells, t cell subsets, killer (k) or natural killer (nk) cells, and the ia antigen were used for cell typing. macrophages were identified by the acid phosphatase reaction. few extravascular mononuclear ...	1984	6383191
molecular mechanisms of chemical mutagenesis: 9-aminoacridine inhibits dna replication in vitro by destabilizing the dna growing point and interacting with the dna polymerase.	9-aminoacridine was found to inhibit dntp incorporation into dna homopolymer duplexes by phage t4 dna polymerase in vitro. systematic variation of the molar ratio of 9-aminoacridine to dna, to dna polymerase, and to dna precursors demonstrated that this inhibition at 9-aminoacridine concentrations below 10 microm was mainly due to interaction of 9-aminoacridine with the dna and suggested that the basis for the preferential inhibition of incorrect precursor incorporation was destabilization of th ...	1984	6383465
hypothyroidism in the foal.	hypothyroidism in the foal occurs as two entities because of the separate actions of thyroid hormones in regulation of metabolic rate and in cell differentiation. the hypometabolic state which results in inadequate thermogenesis and lethargy, occurs concurrently with a period when thyroid hormone secretion is inadequate. also the severity of the concurrent symptoms is related to the degree of hormone inadequacy as measured by plasma concentrations of free t4 and t3. by contrast, the developmenta ...	1984	6383812
antepartum diagnosis of goitrous hypothyroidism by fetal ultrasonography and amniotic fluid thyrotropin concentration.	ultrasonography of a 26-week-old fetus suggested the presence of a goiter. amniocentesis was performed at 26 and 38 weeks gestation to evaluate thyroid function. an elevated amniotic fluid tsh level was found on both occasions, though amniotic fluid t4, t3, and rt3 levels were similar to those of euthyroid fetuses. the diagnosis of goitrous hypothyroidism was confirmed at birth. therapy with thyroid hormone was instituted on the first day of life. growth and development of the infant have been n ...	1984	6384258
amiodarone treatment of critical arrhythmias in children and young adults.	the majority of sudden cardiac deaths in children occur in patients with prior arrhythmias and an abnormal heart. amiodarone was given to 39 young patients (35 with an abnormal heart) with arrhythmias unresponsive to conventional treatment. their age ranged from 6 weeks to 30 years with nine patients younger than 2 years of age. atrial flutter was present in 16 patients, ventricular tachycardia in 14 patients and supraventricular tachycardia in 9 patients. the most common diagnosis (14 patients) ...	1984	6384328
an analysis of dna repair and recombination functions of bacteriophage t4 by means of suppressors: the role of das.	previous studies have indicated that the bacteriophage t4 das mutations partially suppressed the dna replication defects in gene 46 and 47 mutations. here it is shown that the das mutation also suppresses the dna repair defects but not the dna replication defects of the uvsx and uvsy mutations. in contrast, the das mutation suppressed both the dna replication and repair defects of gene 46 and 47 mutations. these characteristics of das as well as those of the other suppressors, including uvsw(dar ...	1984	6385469
a new suppressor of mutations in the dna repair-recombination genes of bacteriophage t4: sur.	a new mutation designated sur was isolated as a suppressor of a mutation in the uvsx gene of t4 phage. unlike the other suppressors of mutations in genes involved in dna repair and recombination, sur has a wide range, suppressing both dna repair and replication defects in mutations in genes uvsx, uvsy, 46, 47, and 59. however, its suppressor functions may be confined to the uvsx-uvsy dna repair pathway since sur did not suppress a mutation in the denv gene. the sur mutation results in an increas ...	1984	6385470
recognition of trnas by aminoacyl-trna synthetases: escherichia coli trnamet and e. coli methionyl-trna synthetase.	in previous work we identified several specific sites in escherichia coli trnafmet that are essential for recognition of this trna by e. coli methionyl-trna synthetase (metrs) (ec 6.1.1.10). particularly strong evidence indicated a role for the nucleotide base at the wobble position of the anticodon in the discrimination process. we have now investigated the aminoacylation activity of a series of trnafmet derivatives containing single base changes in each position of the anticodon. in addition, ...	1984	6389181
identification and characterization of the alc gene product of bacteriophage t4.	bacteriophage t4 infection rapidly and almost completely inhibits transcription of host and other phage dnas. two processes have been implicated to date in this inhibition: (1) adp ribosylation of the alpha subunits of the rna polymerase, involving gpalt (which is injected with the phage dna) and, later, gpmod; and (2) the action of the t4 alc/unf gene product, synthesized immediately after infection. the latter unfolds the host genome and also blocks transcription of cytosine-containing dna. he ...	1984	6389256
identification of the bacteriophage t4 unf ( = alc) gene product, a protein involved in the shutoff of host transcription.	the introduction of plasmid pr386 into e. coli cells renders them restrictive to the growth of phage t4 unf ( = alc) mutants. this system has been used to isolate unf+ revertants, which, along with the mutant parental strains, have been used to identify the unf gene product by two-dimensional gel electrophoresis. synthesis of the unf gene product, a polypeptide of just over 18,000 daltons in size, begins within 1 min after infection and terminates at about 12 min after infection at 30 degrees. g ...	1984	6389257
electron microscope observations on the substructure of t-even (t2, t4) head.	new preparative methods have been devised which allow electron microscope observation of t-even head substructure, under conditions of negative staining. capsomeric constitution of the head shell could so be confirmed and demonstrated for t2 and t4. in addition capsomere size and shell thickness of the head of both phages have been determined and the structural organization of the dna inside t4 head revealed.	1984	6389899
azaserine: further evidence for dna damage in escherichia coli.	azaserine causes dna damage in stationary-phase cells. in our investigation of this damage, we used strains of escherichia coli differing in repair capabilities to study azaserine-induced dna damage, detected as dna strand breaks by sucrose gradient sedimentation techniques. reduced sedimentation in alkaline and neutral sucrose gradients indicated the presence of both alkali-labile sites and in situ strand breaks. azaserine induced dna single-strand breaks (ssbs) abundantly in all but the reca s ...	1984	6390189
escherichia coli dna photolyase stimulates uvrabc excision nuclease in vitro.	pyrimidine dimers are the major photoproducts produced in cellular dna upon uv irradiation. in escherichia coli there are dark and photorepair mechanisms that eliminate the dimers from dna and prevent their lethal and mutagenic effects. to determine whether these repair mechanisms act cooperatively or competitively in repairing dna, we investigated the effects upon one another of dna photolyase, which mediates photorepair, and uvrabc excision nuclease, an enzyme complex of the uvrabc gene produc ...	1984	6390436
[0.5 mg intrathecal morphine in spinal anesthesia. a double blind study on sensory block, postoperative analgesia and adverse effects].	in a randomized double-blind study the influence of morphine 0.5 mg on the development and regression of spinal anaesthesia, the postoperative analgesia and the side effects were investigated. forty-two patients received an isobaric spinal anaesthesia with tetracaine 20 mg without morphine (n = 19) or with morphine 0.5 mg (n = 23). the sensory blockade was tested by pinprick; the patients evaluated their postoperative pain with an analogue scale. arterial blood gases, respiratory rate, blood pre ...	1984	6390547
the t lymphocyte antigen receptor. a critical review of recent experimental literature.	the search for the true nature of the t-cell receptor for antigen has been impeded by several factors including: (1) the relatively low density of antigen binding receptors on t cells: (2) the lack of large quantities of homogeneous t cells for analysis; (3) the inability to study the t receptor protein independent of the t-cell surface; (4) the greater degree of complexity of the receptor compared to conventional immunoglobulin, and (5) possibly the relatively low binding affinity of the recept ...	1984	6390621
clinical significance of in situ detection of t lymphocyte subsets and monocyte/macrophage lineages in heart allografts.	seventy fresh frozen biopsies of 22 human heart allografts were stained with mouse antihuman monoclonal antibodies (okt-4, okt-8, and okm-1) using the immunoperoxidase method. the numbers of infiltrating cell phenotypes were correlated with patients' clinical status and histopathological diagnoses of the biopsies. at the clinically stable stage the number of okt-4-positive cells (t-4 cells, helper/inducer), okt-8-positive cells (t-8 cells, suppressor/cytotoxic), okm-1-positive cells (m-1 cells, ...	1984	6390825
a fundamental approach to the diagnosis of diseases of the thyroid gland.	technologic advances have given the clinician and the laboratory a large armamentarium of tests permitting measurement of virtually every aspect of thyroid function. ready availability at relatively low cost from clinical laboratories allowed these tests to be applied widely to patients. as this occurred, it became evident that abnormal test results were due to nonthyroidal illness in euthyroid sick patients far more often than true indicators of a thyroid disorder. thus, despite the elegance of ...	1984	6391785
the influence of glucose and insulin infusion on in vitro t4 monodeiodination in rat liver microsomes.		1984	6392059
syndromes related to defective iodothyronine metabolism.	"syndromes related to defective iodothyronine metabolism" have been frequently observed in clinical practice. as for methodological and pathophysiological reasons t4, t3, rt3 and tsh estimations are of limited value in these situations, thus, the interpretation of the laboratory findings becomes frequently difficult. furthermore determining the individual "whole body"- or "organ-thyroid state" requires more than measuring the serum concentrations of thyroid hormones. iodothyronine metabolism is ...	1984	6392060
ontogenesis of iodothyronine-5'-deiodinase. induction of 5'-deiodinating activity by insulin, glucocorticoid, and thyroxine in cultured fetal mouse liver.	to elucidate the regulatory mechanism of ontogenetic development of iodothyronine-5'-deiodinase in the fetal and neonatal period, fetal mouse liver of the 19th day of gestation, in which no iodothyronine-5'-deiodinating activity was detectable, was cultured in dulbecco-vogt medium supplemented with 10% thyroid hormone-depleted fetal calf serum, insulin, hydrocortisone, and thyroid hormones. iodothyronine-5'-deiodinating activity of the homogenate was assessed by the amount of iodide released fro ...	1984	6392342
induction of cytolytic activity by anti-t3 monoclonal antibody. activation of alloimmune memory cells and natural killer cells from normal and immunodeficient individuals.	the ability of the monoclonal antibody directed at the t3 antigen (anti-t3) to induce cytolytic activity was investigated since several agents that can activate t cells induce the acquisition of cytolytic activity in a variety of test systems. pretreatment of human alloimmune memory cells, generated in primary long-term mixed lymphocyte cultures, with anti-t3 resulted in the induction of statistically significant specific secondary cytolytic activity and natural killer (nk) cell-like activity. n ...	1984	6392343
[hormonal response to cold during maximum oxygen consumption in the dog].	in dogs, acute exposure to severe cold (immersion 8-13 degrees c) induced a sustained shivering, a rapid increase in oxygen consumption (vo2) and a progressive colonic temperature fall. with a colonic temperature fall rate of 2-6 degrees c/h, vo2 reached a maximum value (vo2 max), which remained steady for more than 30 min. under these conditions, we studied time-courses of plasma catecholamine, cortisol, insulin, t4, t3 and tsh concentrations, as well as of plasma glucose, ffa, lactic acid and ...	1984	6392506
a separate editing exonuclease for dna replication: the epsilon subunit of escherichia coli dna polymerase iii holoenzyme.	dna polymerase iii (poliii) holoenzyme of escherichia coli has 3'----5' exonuclease ("editing") activity in addition to its polymerase activity, a property shared by other prokaryotic dna polymerases. the polymerization activity is carried by the large alpha subunit, the product of the dnae gene. mutations affecting the fidelity of dna replication in vivo and the activity of 3'----5' exonuclease assayed in vitro are found in the dnaq gene, which specifies the epsilon subunit. to determine whethe ...	1984	6393125
a new class of escherichia coli recbc mutants: implications for the role of recbc enzyme in homologous recombination.	mutants of escherichia coli sensitive to phage t4 gene 2 mutants were obtained following ethyl methanesulfonate mutagenesis. by mapping and complementation analysis, the mutations in each of the six mutants are in recb and recc. by both in vivo and in vitro analyses, the nuclease activity of recbc enzyme is undetectable in these mutants. however, by several other criteria, such as proficiency in recombination, relative resistance to uv radiation, and viability of the cells in the culture, these ...	1984	6393130
regulation of transcription in the segment of the bacteriophage t4 genome containing three of the head protein genes: plasmid-specific and phage chromosome-specific effects.	the activity of the phage t4 late promoters, p22, p23, and p24, is regulated by the gene 33, 45, and 55 products and, through the gene 43 product, by dna replication. the accumulation of rna of opposing polarity, with 5' ends mapping to q22 and q23, as described by t. elliott, g. a. kassavetis, and e. p. geiduschek (virology, 1984, 260-282), is subject to the same regulation. when located on a plasmid, the p23 promoter escapes the gene 43 product dependence.	1984	6393567
effects of short-term thyroxine treatment on pancreatic cytology and responses of blood sugar, serum insulin and serum free fatty acids to epinephrine infusion.	the influence of short-term treatment with l-thyroxine on pancreatic histology and on the responses of glycemia, insulinemia and serum free fatty acids to a continuous l-epinephrine infusion in the absence or presence of alpha- and beta-adrenergic blockade was studied in male dogs. l-epinephrine dosage: 0.06 microgram/kg body weight/min for 55 min. two experimental groups were studied, one treated for 10 days with sodium l-thyroxine, one daily dose of 100 micrograms/kg body weight, the other of ...	1984	6393671
hormonal and environmental interactions on thyroid function in the chick embryo and posthatching chicken.	recent research has focused on developmental changes in thyroxine (t4) metabolism as well as hormonal and environmental interactions upon peripheral monodeiodination. toward the end of incubation and the time of air space membrane perforation, concentrations of 3,3', 5'-triiodothyronine t3 increase more rapidly than t4, while reverse t3 (rt3) decreases. administration of glucocorticoids or prolactin (hormones known to increase at the end of incubation) 2 to 3 days prior to air chamber perforatio ...	1984	6394703
interrelation of the avian immune and neuroendocrine systems.	our understanding of immunology and endocrinology has been enriched by studies with different avian species. numerous experiments have identified the role of the avian thymus and bursa of fabricius in the immune response and the interrelation of these glands and their cellular products with the neural endocrine system. the growth pattern of the thymus and bursa are influenced by steroids. increases in thymi growth appear to be controlled, in part, by fluctuations in thyroid activity. sex-linked ...	1984	6394704
[role of excessive body weight and hormonal changes in the development of the main types of hyperlipoproteinemia].	hyperlipoproteinemia (hlp) was detected in 67.3% of 118 males without chd (with the mean age of 44.7 years) engaged in intellectual occupations. the excessive body weight in this group was twice higher, and blood insulin 1.5 times higher, while the sth levels twice lower as compared to patients with normolipoproteinemia (nlp). the mean concentrations of acth, cortisol, t3, t4 in both groups were equal and did not exceed normal. in males with the ii a type of hlp versus those with the ii b and iv ...	1984	6394867
generalized histiocytosis x in the elderly: a light and electron microscope and monoclonal antibody study.	two elderly female patients are described with generalized histiocytosis x (letterer-siwe disease). in each case, a definitive diagnosis was not established until ultrastructural and immunoperoxidase investigations had been performed. the histopathological findings in skin biopsies from each patient were similar. light microscopy demonstrated a bandlike epidermotrophic cellular infiltrate which included large atypical cells (histiocytosis x cells). electron microscopy showed that these cells con ...	1984	6396195
cell-mediated immunity in nutritional deficiency.	dietary deficiencies of specific nutrients profoundly alter cell-mediated immune responses in man and experimental animals. both moderate and severe deficiencies are associated with significant changes in immunocompetence. diets with inadequate levels of protein, calories, vitamin a, pyridoxine, biotin and zinc result in loss of thymic cellularity. secondary to thymic atrophy, the production of thymic hormones critical for the differentiation of t lymphocytes is reduced, especially in protein-ca ...	1984	6396715
effect of iodine administration on thyroid function in diabetic patients.	iodide-induced hypothyroidism has been observed in subjects treated with compounds with mild antithyroid activity. the hypoglycemic agent tolbutamide belongs to the aminoheterocyclic group, a class of compounds with antithyroid effect. thus it was thought interesting to study the effect of large doses of iodide on thyroid function in diabetics chronically treated with tolbutamide. basal thyroid function as assessed by clinical examination and iodothyronine and tsh concentrations was normal in al ...	1984	6397025
kinetics of incorporation of o6-methyldeoxyguanosine monophosphate during in vitro dna synthesis.	o6-methyldeoxyguanosine triphosphate (m6dgtp), known to be produced in vivo by methylation of deoxyguanosine triphosphate with simple methylating mutagens, is utilized by prokaryotic dna polymerases during in vitro replication of synthetic and natural dna template-primers. a study of the kinetic behavior of m6dgtp during dna replication in vitro and of its effect on dna replication indicates that m6dgtp acts as an analogue of datp with kappm of about 6 microm for escherichia coli dna polymerase ...	1984	6386047
increased hepatic microsomal t3 production in vitro induced by insulin administration in rats in vivo.	rats were treated with insulin infusion over 12 h with or without addition of cycloheximide and compared with controls. t4 to t3 deiodination in microsomal liver fraction was measured. insulin treatment led to an increase in t3 production, whereas this effect was blunted by the inhibition of protein synthesis by cycloheximide. neither serum concentrations of t4 nor the liver content of non-protein-bound sulph-hydryl groups explained the alterations in t3 production. it is strongly suggested that ...	1984	6386278
hormonal responses to high and low planes of nutrition in weanling thoroughbreds.	growth-related skeletal diseases in young horses have been associated with high planes of nutrition, although the mechanisms underlying such an association have not been determined. it is likely that nutrition-induced effects on growth rate or growth quality involve the endocrine system. hormonal and metabolic responses to the ingestion of meals containing either 80% (diet a) or 160% (diet b) of national research council energy and protein recommendations were examined in eight thoroughbred wean ...	1984	6386779
the effect of a bacteriophage t4-induced polypeptide on host rna polymerase interaction with promoters.	after infection of escherichia coli with bacteriophage t4, the host rna polymerase acquires several small phage-induced polypeptides (stevens, a. (1974) biochemistry 13, 493-503) and its alpha subunits get adp-ribosylated by a virus-specific enzyme (zillig, w., mailhammer, r., skorko, r., and rohrer, h. (1977) curr. top. cell. regul. 12, 263-271). the modified polymerase displays changed enzymatic properties including sensitivity to increased salt concentration and a higher transition temperatur ...	1984	6386813
isolation and characterization of precursors in bacteriophage t4 baseplate assembly. iii. the carboxyl termini of protein p11 are required for assembly activity.	the assembly activity and electrophoretic mobility of a t4 bacteriophage baseplate protein, p11, have been found to be affected by digestion with the proteases trypsin, subtilisin and carboxypeptidase y. analysis of the trypsin limit-digestion product of p11 by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and size analysis by high performance liquid chromatography indicate that there is a decrease of approximately 5000 in the molecular weight of the p11 molecule or a loss of 2500 in ...	1984	6387156
identification of bacteriophage t4d gene products 26 and 51 as baseplate hub structural components.	products of two bacteriophage t4d genes, 26 and 51, both known to be essential for the formation of the central hub of the phage tail baseplate, have been partially characterized chemically, and their biological role has been examined. the gene 26 product was found to be a protein with a molecular size of 41,000 daltons and the gene 51 product a protein of 16,500 daltons. the earlier proposal (l. m. kozloff and j. zorzopulos, j. virol. 40:635-644), from observations of a 40,000-dalton protein in ...	1984	6387173
vaccinia virus induces ribonucleotide reductase in primate cells.	infection of monkey kidney (bsc-40) cells with vaccinia virus strain wr resulted in a marked increase in ribonucleoside diphosphate reductase (ec 1.17.4.1) activity as measured by cdp reduction in cell-free extracts. after a synchronous infection, increased activity was detected at 2 h, peaked at 4 to 5 h, and then declined between 6 and 8 h to the endogenous cellular level. the induction, detectable at 0.5 pfu/cell, correlated strongly with multiplicity of infection to 10 pfu/cell and continued ...	1984	6387175
genetic analysis of dar, uvsw, and uvsy in bacteriophage t4: dar and uvsw are alleles.	a genetic study of the t4 dar (dna arrested synthesis restoration) mutations was performed by two- and three-factor crosses. the dar mutations restore the viability of gene 59 mutants. mapping studies of the dar mutations indicated that the dar gene extended over 16 map units. this high recombination frequency is attributed to an increased level of recombination in the dar region. two other mutations, uvsy and uvsw, known to be located in the vicinity of dar, were studied. these studies indicate ...	1984	6387177
the low-t3, low-t4 syndrome of nonthyroidal illness: adaptive temporary hypothyroidism.		1984	6387461
disulfide bond engineered into t4 lysozyme: stabilization of the protein toward thermal inactivation.	by recombinant dna techniques, a disulfide bond was introduced at a specific site in t4 lysozyme, a disulfide-free enzyme. this derivative retained full enzymatic activity and was more stable toward thermal inactivation than the wild-type protein. the derivative, t4 lysozyme (ile3----cys), was prepared by substituting a cys codon for an ile codon at position 3 in the cloned lysozyme gene by means of oligonucleotide-dependent, site-directed mutagenesis. the new gene was expressed in escherichia c ...	1984	6387910
treatment of acute graft-versus-host disease with monoclonal antibody okt3. clinical results and effect on circulating t lymphocytes.	eight recipients of a bone-marrow graft from hla-identical, mlr-nonreactive sibling donors who had developed grade ii-iv acute graft-versus-host disease (agvhd), were given 14 consecutive daily injections of 5 mg of a murine anti-t-cell monoclonal antibody (mca) called okt3. four patients with grade ii agvhd showed a complete response; two patients with grade ii had a partial response, and two patients (one with grade ii and one with grade iv) showed no improvement at all. the main side effect w ...	1984	6388068
kinetics and mechanism of dissociation of cooperatively bound t4 gene 32 protein-single-stranded nucleic acid complexes. 1. irreversible dissociation induced by sodium chloride concentration jumps.	the dissociation kinetics of cooperatively bound bacteriophage t4 gene 32 protein from a variety of single-stranded homopolynucleotides has been investigated by stopped-flow techniques. irreversible dissociation of the complexes was induced by rapidly increasing the salt concentration and monitoring the increase in tryptophan fluorescence upon dissociation of the gene 32 protein. the dependence of the apparent dissociation rate constant on initial fractional saturation of the nucleic acid lattic ...	1984	6388631
kinetics and mechanism of dissociation of cooperatively bound t4 gene 32 protein-single-stranded nucleic acid complexes. 2. changes in mechanism as a function of sodium chloride concentration and other solution variables.	the dissociation kinetics of bacteriophage t4 coded gene 32 protein-single-stranded nucleic acid complexes have been examined as a function of monovalent salt concentration, temperature, and ph in order to investigate the details of the dissociation of cooperatively bound protein. fluorescence stopped-flow techniques were used, and irreversible dissociation was induced by a combination of [nacl] jumps and mixing with excess nucleic acid competitor. this made it possible to directly investigate t ...	1984	6388632
direct in vitro effect of thyroid hormones on 25-hydroxyvitamin d3 metabolism in the perfused rat kidney.	we have shown that thyroid hormones have a direct effect on the renal metabolism of 25-hydroxyvitamin d3 (25ohd3). using a new high performance chromatographic technique based upon a cyano-bonded packing, we studied the metabolism of [26,27-3h]25ohd3 in the isolated perfused rat kidney in vitro. we measured the rate of synthesis of 24,25-dihydroxyvitamin d3 [24,25-(oh)2d3], 24-oxo-25-hydroxyvitamin d3 [24-oxo-25ohd3], and 24-oxo-23,25-dihydroxyvitamin d3 [24-oxo-23,25-(oh)2d3] by the kidneys of ...	1984	6418528
measurement of free thyroxine and free triiodothyronine in thyrotoxicosis and hypothyroidism.	free t4 and free t3 concentrations in thyrotoxic and hypothyroid patients at presentation differentiated those with thyroid disease from euthyroid control subjects and were closely correlated with t4/tbg ratio. a further group of thyrotoxic patients was followed serially during treatment with carbimazole. measurements of free t4 and free t3 against closely reflected thyroid status with changes in the hormone measurements being paralleled by changes in t4/tbg ratio.	1984	6420093
serum triiodothyronine and hyperthyroidism in a population sample of women.	the serum t3 assay has been regarded as the most sensitive single test for hyperthyroidism although impaired conversion of t4 to t3 in non-thyroidal illness (nti) might decrease its diagnostic sensitivity. the present report gives experience from the t3 assay in middle-aged females under conditions similar to those in a general health survey. the assays were performed during two periods with an interval of six years. in 1974-75 we studied a representative sample (n = 1283) of women of ages 44, 5 ...	1984	6420094
measurement of free thyroxine and free triiodothyronine in asymptomatic subjects with high concentrations of serum tsh and a history of radioiodine therapy.	total and free thyroid hormones were measured in a group of asymptomatic subjects with high circulating tsh concentrations and a previous history of radioiodine therapy. subjects in the high tsh group all fell within the normal range for measurements of total t4, total t3 and t4/tbg ratio. concentrations of free t4 or free t3 were below the normal range in more than half (9 out of 17) of the high tsh group suggesting that these measurements are more sensitive indices of thyroid failure than meas ...	1984	6430600
alteration of hormone levels in normal males given the anabolic steroid stanozolol.	anabolic steroids have widespread metabolic effects but, to date, their proven clinical indications have been limited. recently the 17 alpha-alkylated steroid, stanozolol, has been shown to be of value in a variety of commonly occurring vascular diseases. its endocrine effects have received little attention and we have investigated the effect of administering a 14 d course of stanozolol (10 mg orally per day) on a variety of important hormonal pathways in nine healthy male subjects. significant ...	1984	6430603
thyroid hormones regulate hepatic glycogen synthase.	short term (48 h) in vivo administration of either t3 or t4 was associated with an increase in hepatic glycogen synthase activity in the rat. administration of 0.25 mg t3/kg bw 48, 24, and 2 h before enzyme preparation increased the total glycogen synthase activity by approximately 50% and increased the percentage of synthase in the i or active form from 19 +/- 1% to 63 +/- 3%. these effects of thyroid hormones on glycogen synthase were rapid (expressed within 24 h) and dose dependent with respe ...	1984	6430678
thyrotropin-releasing hormone stimulates body weight gain and increases thyroid hormones and growth hormone in plasma of cockerels.	the effect of trh on growth in 4-week-old cockerels was examined in two separate experiments. daily injection of trh via the brachial vein stimulated growth in 4-week-old cockerels over 17 days of treatment in the first experiment and over 25 days in the second. in the first experiment, trh at 1.0 micrograms and 10.0 micrograms/bird caused significant (p less than 0.05) increases of 12.0% and 12.4%, respectively, in growth rate, whereas in the second experiment, only the 1.0 micrograms/bird leve ...	1984	6430679
hormone levels of anterior pituitary gland and serum in nagase analbuminemia rats.	the hormone levels in the anterior pituitary gland and serum in nagase analbuminemia rats (nar), a mutant strain established from sprague-dawley rats with hyperlipidemia, were examined. for the anterior pituitary gland, the prolactin, tsh, gh, lh and fsh contents in male nar were significantly lower than those of normal rats. in female nar, prolactin, tsh and lh levels were also lower than those in normal rats, whereas fsh and gh were normal. for the serum, the concentrations of tsh, total t3, t ...	1984	6430688
impaired pituitary thyrotroph function in uncontrolled type ii diabetes mellitus: normalization on recovery.	altered thyroid hormone metabolism with decreased serum t3 and increased rt3 concentrations in patients with uncontrolled diabetes mellitus has been well documented. however, data regarding tsh secretion are sparse, especially the influence of glycemic control. therefore, we examined serum t4, free t4, t3, rt3, t3 resin uptake, and tsh as well as the tsh response to trh administration [expressed as tsh increment (delta tsh) and area under the curve (theta tsh)] in 29 newly discovered type ii dia ...	1984	6430948
paradoxical response of thyrotropin to l-dopa and presence of dopaminergic receptors in a thyrotropin-secreting pituitary adenoma.	we studied the dopaminergic control of tsh secretion in a patient with hyperthyroidism due to a tsh-secreting pituitary adenoma. a 34-yr-old previously thyroidectomized woman had mild clinical hyperthyroidism and a diffuse goiter without exophthalmos. she complained of headaches and had bitemporal hemianopsia. serum t4 and t3 by ria were elevated, and tsh was 112 microu/ml (normal range, 1.1-7.2). the alpha-subunit to tsh molar ratio was 1.7 (normal, less than 1), as reported in other patients w ...	1984	6430950
thyroid hormone abnormalities at diagnosis of insulin-dependent diabetes mellitus in children.	comprehensive evaluation of thyroid hormone indices was performed in 58 children with insulin-dependent diabetes mellitus (iddm) at the time of diagnosis and prior to insulin therapy. two patients were found to have primary hypothyroidism, with markedly elevated tsh and very low t4, free t4, t3, and reverse t3 concentrations. the remaining 56 patients had the transient alterations in thyroid hormone indices that are characteristic of "euthyroid sick" or "low t3" syndrome. mean tsh and reverse t3 ...	1984	6431066
the thyroid function in young men during prolonged exercise and the effect of energy and sleep deprivation.	thyroid function has been investigated in 24 young military cadets participating in a 5 d ranger training course with heavy physical exercise, calorie deficiency and deprivation of sleep. the cadets were divided into three groups, each differing in the amount of sleep and food consumption. the serum levels of thyroid hormones (t4, ft4, t3, rt3) and tbg showed a biphasic pattern during the course. initially there was an increased secretion concomitant with an increased deiodination of t4 to t3 an ...	1984	6432374
calcitonin inhibits trh-induced tsh secretion.	the authors investigated in 14 healthy volunteers the effect of a single dose of 50 mrcu of synthetic salmon calcitonin on plasma levels of thyroxine (t4), triiodothyronine (t3), thyrotropin (tsh), calcium, phosphorus and magnesium and on the thyrotropic secretory reserve assessed by means of the trh test. t4 and t3 levels did not change significantly within 120 min after administration of calcitonin. tsh levels during the 60th minute declined insignificantly. trh stimulated tsh levels after adm ...	1984	6432564
effects of anti-thyrotropin-releasing hormone anti-serum treatment during the neonatal period on the development of rat thyroid function.	effects of anti-thyrotropin-releasing hormone (trh) anti-serum treatment during the neonatal period on the development of rat thyroid function were studied. on postnatal days 2 and 4, rats were administered anti-trh anti-serum ip, and they were serially decapitated at the 4th, 8th and 12th week after birth. trh, thyrotropin (tsh), thyroxine (t4) and 3,3',5-triiodothyronine (t3) were measured by radioimmunoassay. immunoreactive trh (ir-trh) in the hypothalamus did not change significantly after a ...	1984	6432670
effects of long-term amiodarone treatment on thyroid function.	serum determinations of total t4, total t3, reverse t3, free t3 index, free t4, free t4 index, tbg, t4/tbg ratio, tsh and cholesterol were carried out on 18 euthyroid patients with coronary heart disease. serum samples were obtained before treatment and after 15 days, 2, 4, 6, 8, 10, 12, 14 and 16 months of treatment with amiodarone (400 mg/day). patients were divided into two groups, according to patterns of tsh response to thyrotrophin-releasing hormone (trh): (i) patients with normal response ...	1984	6432715
human cytolytic t lymphocyte interactions with vascular endothelium and fibroblasts: role of effector and target cell molecules.	monoclonal antibodies (mab) against cell surface structures have been used to identify several molecules involved in the interaction of human cytolytic t lymphocytes (ctl) with lymphoid and other bone marrow-derived targets. in allograft rejection or in graft-vs-host disease, however, major cellular targets are vascular and stromal cells, especially endothelium. yet little is known about whether the same cell surface molecules are involved in the interactions of ctl with these cell types. we ass ...	1984	6432903
functional isotypes are not encoded by the constant region genes of the beta subunit of the t cell receptor for antigen/major histocompatibility complex.	human t cell clones and a cdna probe specific for constant regions of the beta subunit of the antigen/major histocompatibility complex (mhc) receptor, tic beta 1 and tic beta 2, were employed to determine whether these genes were differentially used by functional classes of t lymphocytes. dna from 10 interleukin-2-dependent t cell clones including class i and class ii mhc-specific cytotoxic t lymphocytes (n = 6), t4+ inducer t lymphocytes (n = 2), and t8+ suppressor t lymphocytes (n = 2) showed ...	1984	6432935