Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| detection of cross-reacting murine i-j like determinants on a human subset of t8+ antigen binding, presenting and contrasuppressor cells. | murine i-j gene products have been found in t suppressor cells (sc) and factors, macrophages and contrasuppressor cells (csc). however, a human counterpart of the murine i-j has not been reported. as there is strong evidence that some murine anti-ia antisera cross-react with human ia antigens, the possibility was tested that mouse anti-i-j antibodies might cross-react with corresponding human class ii determinants. indeed, this revealed that three anti-i-j monoclonal antibodies (moab) and two an ... | 1984 | 6209044 |
| the effects of gonadal steroids on the content of substance p in the rat anterior pituitary. | the effects of gonadectomy and of the administration of gonadal steroids on the content of substance p in the anterior pituitary (ap-sp) were studied in adult rats. the effect of gonadal status on the ap-sp content of thyroidectomized (tx) rats was also studied. we have confirmed that the ap-sp content in adult males is higher than that in adult females. ovariectomy (ovx) caused an increase in ap-sp content which was apparent 6 days after surgery. estradiol (e2; 2 micrograms/rat daily) administe ... | 1984 | 6209122 |
| hereditary deficiency of okt4-positive cells: studies for mode of inheritance and lymphocyte functions. | two graves' patients were found to have no okt4+ cells in their peripheral blood lymphocytes (pbl). however, the reactivity of their lymphocytes with t4(t4a) and anti leu-3a was normal and no autoantibodies to the okt4 determinant were found in their sera. cytofluorographic analysis of pbl from their family members showed three types of immunofluorescence profiles with okt4. the first type was the complete okt4+ cell deficiency, the second was the normal percentage of okt4+ cells with half immun ... | 1984 | 6209207 |
| a site in a trna precursor that can be processed by the whole rnase p enzyme but not by the rna alone. | a precursor molecule for 10 sb rna, the rna moiety of the rna processing enzyme rnase p, was purified, characterized for enzymatic activity, and compared to 10 sb rna and to rnase p. in these studies the k rna, a dimeric precursor of trnagln-trnaleu, coded by bacteriophage t4, was used as a substrate. this precursor contains two rnase p cleavage sites, one at each 5' end of the two trnas. the precursor 10 sb and 10 sb rnas have the capacity to cleave the precursor trna molecule but only at the 5 ... | 1984 | 6209276 |
| [effect of deficiency and excess of thyroid hormones in the body on indolamine metabolism in the rat epiphysis cerebri]. | thyroid hormones were demonstrated to play a role in serotonin metabolism control in pinealocytes. saturation of the body with t4 shifts the process of serotonin utilization toward acetylation and subsequent ortho-methylation with primary formation of n-acetylserotonin and melatonin while total thyroidectomy blocks this process to a considerable degree. under deficiency of thyroid hormones the pathway of oxidative serotonin deamination with primary formation of 5-hydroxyindole-acetic acid is the ... | 1984 | 6209708 |
| radiotherapy of t4 bladder carcinoma. | a 16% 5-year crude survival was observed in 159 irradiated patients with t4nxmo bladder carcinoma. the presence of a t4a tumour and a good performance status were important prognostic parameters. the combination of radiotherapy and weekly injections of 5-fura (12 mg/kg) resulted in a significant 2-year survival increase. new regimens of combined radiotherapy/chemotherapy should be developed for patients with t4nxmo bladder carcinoma. the palliation effect of radiotherapy should further be evalua ... | 1984 | 6209751 |
| the semi-quantitative distribution of t4 and t6 surface antigens on human langerhans cells. | we have used indirect immunogold electron microscopy to compare the respective density of cell membrane determinants revealed by okt6 and okt4 monoclonal antibodies on normal human langerhans cells (lc): 12.9 +/- 3.5 gold granules were noted per cell section on okt4-positive lc whereas 236.8 +/- 23.5 granules were counted per cell section on okt6-reactive cells. these results confirm that human lc react with okt4 antibody and they demonstrate a marked quantitative difference on lc surface betwee ... | 1984 | 6210099 |
| effects of thyroxine and 3,5,3'-triiodothyronine in brown adipose tissue of rats. | rats of both sexes were either cold acclimated (6 +/- 1 degree c) or treated with thyroxine (t4) or 3,5,3'-triiodothyronine (t3) (500 micrograms/kg body wt daily s.c. for 3 weeks). wet weight, total proteins, lipids and nucleic acids in the interscapular brown adipose tissue (ibat) were measured. values obtained with t4 treatment were similar to those obtained with t3 treatment. t3 is the main thyroidal hormone in the rat and it is formed from t4 deiodination in liver and kidney. as t4-treated r ... | 1984 | 6210174 |
| immunoregulatory subsets of the t helper and t suppressor cell populations in homosexual men with chronic unexplained lymphadenopathy. | unexplained, generalized lymphadenopathy in homosexual men, which can be a prodrome to the acquired immunodeficiency syndrome, is associated with impaired cell-mediated immunity, a low ratio of t helper-inducer to t suppressor-cytotoxic cells (defined by the t4 and t8 monoclonal antibodies), and hypergammaglobulinemia. we performed double-marker studies on t cells by using a panel of monoclonal antibodies (ia, t17, tq1, and leu-8), which reportedly detect activation or functional subsets of the ... | 1984 | 6228564 |
| total lymphoid irradiation in kidney and liver transplantation in the baboon: prolonged graft survival and alterations in t cell subsets with low cumulative dose regimens. | prolonged kidney and liver allograft survival was produced in baboons by low cumulative doses (500 to 1200 rad) of total lymphoid irradiation (tli). continuing normal graft function for more than 1 yr after transplantation was seen in 10 animals in this series. the longest survivor (greater than 4 years) rejected a third party kidney allograft in typically acute fashion 1 yr after the original transplantation. the dose-response effect was parabolic, with a cumulative dose of 800 rad given as twi ... | 1984 | 6228586 |
| the t4 surface antigen is involved in the induction of helper function. | the okt4 monoclonal antibody reacts with a 62k m.w. glycoprotein present on a subset of human t cells with the capacity to help or induce b cell differentiation. the present studies were undertaken to determine whether the t4 molecule itself plays any role in the helper function mediated by t4+ cells. using a series of monoclonal antibodies (okt4, okt4a-e), which react with distinct noncompeting epitopes of the t4 molecule, we found that okt4a and okt4e, but not okt4, antibodies inhibited the in ... | 1984 | 6228598 |
| t-all related to intermediate cortical/medullary thymocytes displaying functional and surface characteristics of suppressor t cells. | leukemic cells of a patient with acute t-lymphoblastic leukemia (t-all) exhibiting the uncommon clinical feature of hypogammaglobulinemia were examined in terms of surface markers and immunologic functions. employing various monoclonal reagents reacting with surface antigens present on t cells and additional conventional markers, it was shown that the patient's leukemic cells expressed a phenotypical profile (e-r+, tdt+, c3d-r-, okt3-, t4-, t6(+), t 10+, t8++) corresponding to the intermediate s ... | 1984 | 6229130 |
| immunologic abnormalities in patients receiving multiple blood transfusions. | concern for the potential risk of the acquired immunodeficiency syndrome among blood cell recipients led us to measure immunologic functions in patients who had received multiple transfusions. abnormalities of two immunologic tests, natural killer cell function and helper/suppressor (t4/t8) lymphocyte subpopulation ratio, have characterized patients with the acquired immunodeficiency syndrome and are prevalent in populations at risk for this syndrome. natural killer cell function was severely de ... | 1984 | 6229206 |
| lung t cells in hypersensitivity pneumonitis. | monoclonal antibodies okt3 (all t cells), okt4 (t-helper/inducer), and okt8 (t-suppressor/cytotoxic) were used to determine surface phenotypes of bronchoalveolar lavage and peripheral blood lymphocytes in patients with chronic hypersensitivity pneumonitis. similar studies were done in asymptomatic pigeon breeders, patients with sarcoidosis, and nonsmoking controls. increased numbers of lavage t cells were found in patients with hypersensitivity pneumonitis and sarcoidosis and in asymptomatic pig ... | 1984 | 6230036 |
| reactivity of inducer cell subsets and t8-cell activation during the human autologous mixed lymphocyte reaction. | to characterize the responding t cells in the autologous mixed lymphocyte reaction (amlr), t cells were fractionated into purified subpopulations employing monoclonal antibodies and a variety of separation techniques including fluorescence-activated cell sorting. it was found that isolated t4 cells, but not t8 cells, proliferated in response to autologous non-t cells. more importantly, within the t4 subset, the autoreactive population was greatly enriched in a fraction reactive with an autoantib ... | 1984 | 6230184 |
| t cell regulation of polyclonally induced immunoglobulin secretion in humans. | we measured the pokeweed mitogen (pwm)-induced secretion of igg by the unfractionated mononuclear cells (mnc) of young adult donors, and correlated the results with the functional activity of cell suspensions enriched for t helper (t4+) and t suppressor/cytotoxic (t8+) cells. the distribution of igg levels secreted by mnc differs from a gaussian curve, implying that the group is composed of distinct heterogeneous populations. when donors were compared who were judged to be very low responders or ... | 1984 | 6230393 |
| [t3-t4 magic formula]. | 1984 | 6230655 | |
| failure to distinguish ultrastructurally between t4+ (helper) and t8+ (suppressor/cytotoxic) t-cell subsets. | human peripheral t-cell subpopulations revealed by monoclonal antibodies by means of a rosetting method were isolated by micromanipulation and submitted to electron microscopic analysis. the t3+ subset (total t cells) displayed a high degree of heterogeneity, including multiple transitional forms, from cells with a high nuclear to cytoplasmic ratio and rare organelles to cells with a low nuclear to cytoplasmic ratio and a complex system of cytoplasmic organelles. t4+ (inducer/helper) and t8+ (su ... | 1984 | 6230715 |
| effect of t4-induced cardiac hypertrophy on o2 supply-consumption balance during normoxia and hypoxia. | the purpose of this study was to determine if thyroxine-induced hypertrophic hearts can maintain an adequate o2 supply-consumption balance both at rest and under hypoxic stress. new zealand white rabbits were given 0.5 mg/kg l-thyroxine (t4) for 3 or 16 days, and a third group served as a control. chests were opened under anesthesia, and myocardial blood flow was determined using microspheres. in half of these animals, microspectrophotometric determinations were made on left ventricular arterial ... | 1984 | 6230939 |
| rabbit myocardial membrane ca2+-adenosine triphosphatase activity: stimulation in vitro by thyroid hormone. | the in vitro stimulation of human and rabbit erythrocyte membrane ca2+-atpase activity by physiological concentrations of thyroid hormone has recently been described. to extend these observations to a nucleated cell model, ca2+-atpase activity in a membrane preparation obtained from rabbit myocardium has been studied. activity of 5'-nucleotidase in the preparation was increased 26-fold over that of myocardial homogenate, consistent with enrichment by sarcolemma. mean basal enzyme activity in mem ... | 1984 | 6230996 |
| diurnal variations of plasma growth hormone, thyrotropin, thyroxine, and triiodothyronine in streptozotocin-diabetic and food-restricted rats. | the pattern of spontaneous gh, tsh, t4, and t3 secretion has been studied in male rats in response to a 15-day period of streptozotocin diabetes or food restriction. beginning at 0900 h, groups of control (c), food-restricted (fr), diabetic (d), and insulin-treated d rats were killed every 60-90 min for a 8-h period. food restriction resulted in a significant depression of the gh, tsh, t4, and t3 peaks, whereas diabetes caused complete suppression of episodic secretion of each hormone. insulin ( ... | 1984 | 6238818 |
| helper function of human t cells with different affinity for helix pomatia a hemagglutinin in a tetanus toxoid-induced b cell differentiation system. | t cells from human peripheral blood were enriched in t4+ cells by lysis of t8+ cells with the monoclonal antibody okt8 plus complement. the t4+ subset was separated into 4 fractions differing in avidity for the lectin helix pomatia a hemagglutinin (hp). the fractions were studied for their capacity to help autologous b cells to differentiate and mature into immunoglobulin (ig) synthesis and secretion after activation with tetanus toxoid (tt) in vitro. to ensure the antigen specificity of inducti ... | 1984 | 6238830 |
| [lymphocytes and lymphocyte subpopulations]. | human lymphocytes can be subdivided in several subgroups by means of their membrane markers. t-lymphocytes, b-lymphocytes and natural killer cells can be enumerated with monoclonal antibodies reacting with surface glycoproteins or with antibodies against surface immunoglobulins. the analysis of the membrane antigens and receptors leads to a better understanding of lymphocyte differentiation from stem cells to immunocompetent mature cells. t-lymphocytes can be subdivided in a helper cell fraction ... | 1984 | 6238902 |
| [chronic lymphocytic leukemia of t-cells with a suppressor phenotype (t3+, t4-, t8+). report of 2 cases]. | 1984 | 6239401 | |
| a reappraisal of the effects of monoclonal antibodies directed at t cell differentiation antigens. | we examined the effects of monoclonal antibodies (abs) directed at t3 antigen (expressed on most post-thymic t cells), t4 antigen (helper/inducer subset) and t8 antigen (suppressor/cytotoxic subset). anti-t3 induced interleukin-2 production and proliferation of peripheral blood mononuclear cells (pbm). anti-t4 or t8 did not exhibit such properties. addition of methylprednisolone (mp) or cyclosporine (csa) to pbm activated with anti-t3 resulted in 79% and 88% suppression of proliferation, respect ... | 1984 | 6239416 |
| [lymphocyte subpopulations (t4-t8) in postoperative peritonitis]. | 1984 | 6240647 | |
| recent progresses in the phenotypic and functional characterization of human cytolytic t lymphocytes. | over the past several years, a number of major advances have been made in our understanding of the phenotypic and functional heterogeneity of human t lymphocytes. most of these advances have resulted from: 1. the availability of monoclonal antibodies directed to t cell surface antigens; 2. the development of sensitive microassays to measure lymphocyte function in vitro; 3. the refining of t cell cloning technology. the most relevant studies in delineating how human t cell functions have been bas ... | 1984 | 6240762 |
| [characterization of a series of monoclonal antibodies against human t cells]. | a set of 6 monoclonal antibodies is characterized reacting only with human t-cells but not with b lymphocytes. the antibody bl-t1 binds to the e receptor, being there on all mature t cells and on the majority of thymocytes. all respectively the majority of peripheral t cells but only subpopulations of thymocytes are recognized by the monoclonals bl-t2, bl-t3, bl-t4 and bl-t5. for identification and quantification of mature human t cells especially the antibody bl-t2 is suitable. the antigen reco ... | 1984 | 6240938 |
| [radioimmunoassay of t3, t4, t3u, fti and tsh in diagnosis of thyroid diseases]. | 1984 | 6241093 | |
| characterization of cellular infiltrates in skin lesions of atopic eczema by means of monoclonal antibodies. | frozen skin biopsies from 3 acute and 3 chronic lesions of atopic dermatitis were examined by means of various ortho-monoclonal antibodies (okt3, 4] not equal to----4, okt6, okt8; okia and okm1) in an indirect immunofluorescence technique. the results show that both t helper (t4) and t suppressor cytotoxic (t8) lymphocytes are present in the inflammatory infiltration which predominantly contained ia mononuclear cells. despite the absence of double staining procedure, the observations suggest tha ... | 1984 | 6241157 |
| impaired immune regulation in children and adolescents with hemophilia and thalassemia in israel. | the immune function was assessed in 22 children, adolescents and young adults with asymptomatic hemophilia, and 15 with thalassemia, in israel. five patients with hemophilia and two with thalassemia were found to be severely abnormal, having cutaneous anergy, very low t-helper cells, elevated t-suppressor cells, inverted t-helper/suppressor ratio, reduced response to mitogens and antigens, and nonfunctional nk cells. four of the five hemophilia patients exhibited profound lymphopenia also. decre ... | 1984 | 6241995 |
| immunologic studies of the acquired immune deficiency syndrome: relationship of immunodeficiency to extent of disease. | immunologic and conventional laboratory studies were done in 135 previously untreated subjects including 28 (20.7%) symptom-free homosexuals and 74 (54.8%) with arc and 33 (24.5%) with aids. more than half of all patients had abnormal percentages of lymphocytes, percentages of t3+ cells, percentage and absolute number of t4+ cells, percentage of t8+ cells, t4/t8 ratio, monocyte adherence, and serum thymosin alpha 1. most immune variables worsened with progressive disease. low lymphocyte 5'nucleo ... | 1984 | 6242001 |
| [value of the perifusion of fragments of rat thyroid for the dynamic study of thyroid secretion under tsh and theophylline]. | with the technique of rat thyroid fragment perifusion the kinetics of t4 response to various stimuli can be studied with precision. the response is rapid and brief with theophylline and tsh, which activate membrane adenylcyclase; with the optimal concentrations, however, it is prolonged because only these concentrations can induce the secretion of a more slowly releasable pool of t4. | 1984 | 6242029 |
| [importance of the excretion of triiodothyronine (t3) in normal infants and children]. | serum concentrations of thyroxine (t4) and triiodothyronine (t3) are higher in normal neonates and children than in adults, having a thyroxine to triiodothyronine ratio close to 50. t3 excretion is almost as high as that of t4 (t4 to t3 ratio from 0.6 to 0.8 in children). this amount of excreted t3 represents a loss of about 1/10 of the theoretical daily production rate of the hormone. | 1984 | 6242030 |
| immunoperoxidase evaluation of lymph nodes from acquired immune deficiency patients. | immunoperoxidase evaluation of cryostat sections from acquired immune deficiency syndrome (aids) or prodromal nodes indicated that the majority (75%) had t8 dominance as contrasted to reactive hyperplasia nodes of unknown origin which had 80% t4 dominance. the cells in the aids nodes in the interfollicular and medullary sinuses areas marked intensely for ia as opposed to sparse scattering in the comparative group. finally, the follicles in the study group as opposed to the comparative group demo ... | 1984 | 6231145 |
| direct mitogenic effect of ionophore a23187 on isolated human t helper lymphocytes. | the ca2+ ionophore a23187 induces only a weak mitogenic response in cultures of unfractionated mononuclear leukocytes from human blood. when a comitogen, 12-o-tetradecanoyl-phorbol-13-acetate, which by itself is nonmitogenic, is added, a greatly increased cell proliferation is obtained. highly purified t lymphocytes respond by proliferation to a23187 alone. studies on functional subsets of t cells, fractionated by using the monoclonal antibodies okt4/okt8, revealed that a23187 is a strong mitoge ... | 1984 | 6231189 |
| relationship between systemic lupus erythematosus t cell subsets, anti-t cell antibodies, and t cell functions. | previous studies have shown that patients with systemic lupus erythematosus (sle) had differing t cell t4+/t8+ ratios and that the ratio correlated with clinical features of the disease. in the present study, we wished to determine whether the peripheral blood t cell subsets in these patients were related to the specificity of anti-t cell antibodies found in their plasma. plasma from 24 sle patients that reacted with greater than 20% of normal t cells were analyzed for their effect on in vitro p ... | 1984 | 6231307 |
| treatment of prostatic cancer: the eortc experience--preliminary results of prostatic carcinoma trials. | six prospective studies in the field of prostatic carcinoma have been carried out to date by the eortc urological group. in three phase ii studies, adriamycin, procarbazine, vindesine, and mitomycin c have been studied. two of the three protocols have been completed. in three phase iii studies, 3 mg of diethylstilbestrol (des) is compared to cyproterone acetate (cpa), medroxyprogesterone acetate (mpa), and estramustin phosphate (estracyt). these two protocols have been closed to entry. the curre ... | 1984 | 6231535 |
| prednisone and t-cell subpopulations. | alteration of t-cell subset relationships may cause many of the anti-inflammatory and immunoregulatory effects of glucocorticosteroids. the effect of oral administration of lactose or 60 mg of prednisone on peripheral blood t-lymphocyte subset profile and total eosinophil count (tec) was examined. a purified t-cell peripheral blood population was obtained and the proportion of t cells with t3, t4, t8, m1, and la surface antigens was determined before and five hours after ingestion of lactose or ... | 1984 | 6231898 |
| suppressor cells in end-stage renal disease. functional assays and monoclonal antibody analysis. | suppressor cell activity after concanavalin a induction was studied in peripheral blood mononuclear cells of patients undergoing long-term hemodialysis. suppression both of the mixed lymphocyte reaction and of allogeneic cells stimulated with phytohemagglutinin was significantly higher with peripheral blood mononuclear cells from patients undergoing hemodialysis than with cells from control subjects. expression of the ia antigen on t lymphocytes (associated with immunologic activation) was studi ... | 1984 | 6232846 |
| cutaneous lymphomatoid granulomatosis: a rare cause of recurrent skin ulceration. | we report the case of a 34-year-old male who presented with recurrent skin ulceration due to lymphomatoid granulomatosis. monoclonal antibody studies showed a high t helper (t4):t suppressor (t8) cell ratio in both the lesions and the peripheral blood. plasma cells in the infiltrate showed iga lambda staining but as yet no monoclonal immunoglobulin has been identified in the blood or urine. lymphocytes bearing markers of immature cells (t10) were observed in the tissue and blood. | 1984 | 6232940 |
| predictive value of excretory urography, ultrasonography, computerized tomography, and liver and bone scan in the staging of bilharzial bladder cancer in saudi arabia. | the role of ultrasonography, computed tomography (ct), and radioisotopic scanning in the staging of bilharzial bladder cancer has not been reported previously. forty patients with invasive bladder cancer seen at the king faisal specialist hospital and research centre between january 1978 and june 1981 underwent complete preoperative workup for staging of their tumors prior to radical cystectomy. the preoperative radiologic investigations included excretory urography (ivp), ultrasonography (us), ... | 1984 | 6232995 |
| helper t cell activation for the human b cell response to trinitrophenylated polyacrylamide beads: involvement of the t4 antigen. | the monoclonal antibody (mab) okt4a (but not okt4) inhibits the in vitro antibody response of human peripheral blood mononuclear cells. the target of okt4a mab is the helper t cell, as the helper cells for the antibody response to trinitrophenylated polyacrylamide beads (tnp-paa) are exclusively in the t4 subset. the okt4a mab is still suppressive when the anti-tnp response of cultures of monocyte-depleted cells is supported by purified interleukin 1. both the okt4a mab and anti-dr mab suppress ... | 1984 | 6233157 |
| a monoclonal antibody that blocks class ii histocompatibility-related immune interactions. | previous studies using conventional hetero- or isoantisera have indicated the involvement of class ii (ia) molecules in presentation of soluble antigen by monocytes to inducer t lymphocytes, stimulation of inducer t cells in mlr, and recognition of ia-bearing target cells by cytotoxic t lymphocytes (ctl). the experience in using monoclonal anti-ia reagents capable of blocking these phenomena in the human system is limited. recently, however, we have characterized a lytic igg2a monoclonal antibod ... | 1984 | 6233239 |
| analysis of t regulator cell surface markers and functional properties in multiple sclerosis. | the relative proportions as well as cell surface and functional properties of t suppressor (t8+) and t helper (t4+) cells in peripheral blood mononuclear cells ( mncs ) of ms patients were analyzed. the proportion of t8 cells compared to normal controls was suggestively lower in patients during relapses and significantly lower in those with progressive disease. the density of t8+ antigen on cells of ms patients with active disease as measured by median fluorescence intensity ( mfi ) was also dec ... | 1984 | 6233299 |
| rapidly expanded activated human killer cell clones have strong antitumor cell activity and have the surface phenotype of either t gamma, t-non-gamma, or null cells. | cloned lymphoid cell lines showing cytolytic activity were derived from natural killer (nk) cell-enriched cell fractions obtained by fluorescence-activated cell sorting of cells that reacted with b73 .1, an nk cell-specific monoclonal antibody (mca). the clones were cultured for more than 30 generations (i.e., more than 10(9) descendants from a single cell). the rapid expansion was achieved by using a special culture system developed for this purpose and based on the use of two types of allogene ... | 1984 | 6233369 |
| identification of the cellular subpopulations infiltrating rejecting cadaver renal allografts. preponderance of the t4 subset of t cells. | in the rejection response against renal allografts, the relative importance of helper/inducer t cells mediating a delayed-type hypersensitivity response and of t cells with direct cytotoxicity has not been defined. these subpopulations were identified with commercially available monoclonal antibodies and an indirect immunoperoxidase technique in 31 renal biopsies from patients undergoing acute rejection episodes and in 9 rejected nephrectomy specimens. t lymphocytes were the predominant cell pop ... | 1984 | 6233769 |
| [evaluation of the immune system in down's syndrome patients]. | the authors carry out a study to evaluate the immunoglobulin and complement factor levels and the populations and subpopulations of mononuclear cells enumerated by monoclonal antibodies, in down's syndrome (ds) patients. the igg, iga and igm immunoglobulins were significantly increased, and presented an age-dependence. the complement factors, c3 and c4 were normal. we enumerated t lymphocyte subsets in these patients using okt3, okt4, okt6, okt8, okia1 and okm1 monoclonal antibodies. the percent ... | 1984 | 6233858 |
| diminished helper/suppressor lymphocyte ratios and natural killer activity in recipients of repeated blood transfusions. | immunologic abnormalities qualitatively similar to those seen in acquired immunodeficiency syndrome (aids), including a low helper/suppressor lymphocyte ratio and low natural killer (nk) activity, have been observed in many hemophiliacs receiving clotting factor concentrates. to determine whether these changes also occur after repeated blood transfusion, we measured helper/suppressor (t4/t8) ratios and nk activity in four groups of test subjects: (a) 30 subjects with sickle cell anemia (sca) rec ... | 1984 | 6234037 |
| changes in mitogen responsiveness lymphocyte subsets after traumatic injury: relation to development of sepsis. | head injury and multiple trauma patients were evaluated for mitogen responsiveness and lymphocyte subset frequencies within the first few days after injury. the profile obtained was compared to the patient's clinical course to see if there was a relation between early immune abnormalities and the subsequent development of unanticipated sepsis. lymphocytes from multiple trauma patients were generally hyporesponsive to in vitro stimulation with a suboptimal dose of the mitogen phytohemagglutinin ( ... | 1984 | 6234119 |
| internal motion of dna in bacteriophages. | we have investigated internal motion of dna in bacteriophages by measuring fluorescence anisotropy decays of intercalated ethidium. the results showed large suppression of the internal motion of the inner dna; the interhelix interaction of the dna in the phage head is considered to enhance the effective viscosity of the dna rod and to restrict the angle of the internal motion. considering that the observed internal motion arises mainly from torsional motion of the dna, we have calculated the mov ... | 1984 | 6234311 |
| biologic functions of the okt1 t cell surface antigen. i. the t1 molecule is involved in helper function. | the okt1 (leu-1) monoclonal antibody reacts with a 69 kd glycoprotein that is present on only a fraction of functionally mature thymocytes but is maintained on most peripheral t cells. in the study presented, we examined the role of this surface molecule in t cell function. we found that addition of the okt1 antibody to b cells and autologous t4+ cells markedly enhances b cell differentiation. this enhancement was apparent when nonirradiated or irradiated t4+ cells but not t8+ cells were used. m ... | 1984 | 6234357 |
| relationship between immune system and gram-negative bacteria. ii. natural killer cytotoxicity of salmonella minnesota rb 345-unbound human peripheral blood lymphocytes. | spontaneous binding of human peripheral blood lymphocytes (pbl) to bacteria represents a promising approach for the characterization of lymphocyte subsets mediating different functions. in the light of previous findings on the high degree of spontaneous adherence of s. minnesota rb cells to pbl, we have evaluated the natural killer (nk) cytotoxicity of pbl subpopulations that fail to bind to rb bacteria. the s. minnesota rb-unbound cell fraction exhibits higher levels of cytotoxic capacity, whic ... | 1984 | 6234358 |
| t-lymphocytes and the subpopulations of t-helper and t-suppressor cells measured by monoclonal antibodies (t11, t4, and t8) in relation to surgery under epidural and general anaesthesia. | monoclonal antibodies reacting with specific determinants on lymphocytes and their subpopulations were used to study the influence of surgery under general or epidural anaesthesia on the number of t-cell (t11), t-suppressor cells (t8), and t-helper cells (t4). the t-cell fraction decreased significantly during surgery, but was only moderately reduced on the first postoperative day. the change in the t-cell fraction was the same under both types of anaesthesia. the helper and suppressor cell frac ... | 1984 | 6234743 |
| [relation between the iodination of human thyroglobulin and the cleavage of the hormone peptide 26k n-terminal]. | at moderate iodination levels (about 20 iodine atoms/mol) human thyroglobulin yields after reduction and alkylation a hormone (t4)-containing n-terminal peptide of 26k. further iodination of the thyroglobulin in vitro results in the cleavage of this part of the molecule into smaller peptides of 22k and 18k. a precursor-product relationship between the 26k peptide segment and the latter was established by showing an identical n-terminal t4-containing sequence in the 3 peptides. cleavage of peptid ... | 1984 | 6234970 |
| unidirectional digestion with exonuclease iii creates targeted breakpoints for dna sequencing. | a method is described for the rapid generation and cloning of deletion derivatives well-suited for the sequencing of long stretches of dna. this method is based on two useful features of exonuclease iii: (1) processive digestion at a very uniform rate and (2) failure to initiate digestion at dna ends with four-base 3'-protrusions. the method was applied to a 4570-bp drosophila genomic dna fragment cloned in the single-stranded phage vector m 13mp18. an ordered set of deletion clones was made by ... | 1984 | 6235151 |
| surface molecules involved in self-recognition and t cell activation in the autologous mixed lymphocyte reaction. | although the importance of t cell lineage-specific surface glycoproteins and mhc gene products in soluble-, viral-, and alloantigen-stimulated immune responses has been well characterized, those involved in autoreactivity are less defined. to address this issue, we examined the ability of monoclonal antibodies to influence the autologous mixed lymphocyte reaction (amlr). here we show that both monoclonal anti-t3 and several monoclonal anti-t4 antibodies profoundly inhibited the autoreactivity of ... | 1984 | 6235279 |
| interaction of reovirus with cell surface receptors. iv. the reovirus type 3 receptor is expressed predominantly on murine lyt-2,3+ and human t8+ cells. | reovirus type 3 binds to approximately 20% of murine and human t cells via the viral hemagglutinin, a small outer capsid polypeptide. by using purified viral particles as a ligand in a standard plate separation technique, we have been able to enrich human peripheral blood and murine splenic t cells for reovirus receptor-positive cells (reovirus 3+) to levels of 88 to 92%. analysis of reovirus 3+ t cells with monoclonal antibodies that identify inducer and suppressor/cytotoxic cells demonstrated ... | 1984 | 6235291 |
| the complex of t4 bacteriophage gene 44 and 62 replication proteins forms an atpase that is stimulated by dna and by t4 gene 45 protein. | the bacteriophage t4 genome is believed to encode all of the proteins needed for the replication of its own dna. included among these proteins are the "polymerase accessory proteins", the products of t4 genes 44, 62 and 45. the first two of these genes specify the synthesis of the 44/62 protein complex, which is here shown to be a dna-dependent atpase, hydrolyzing either atp or datp to the corresponding nucleoside diphosphate and releasing inorganic phosphate. this nucleotide hydrolysis is great ... | 1984 | 6235378 |
| multiple sclerosis: relation of in vitro igg secretion to t suppressor cell number and function. | the proportion of ms patients whose pokeweed mitogen-stimulated mononuclear cells (mncs) secreted greater than 1,000 ng/ml igg per 10(6) cells (ie, "high responders") was increased compared with controls. the suppressor effect mediated by a constant number of t8+ cells from high responders, both ms and control, on igg secretion by standard t helper (t4+) plus b-cell cultures was significantly lower than that for the same number of t8+ cells from "low responders." the proportion of t8+ cells with ... | 1984 | 6235461 |
| anti-thyroid activity of purified thymus gland extract in male wistar rats. | the effect of a purified bovine thymus gland extract (dr. kurt mulli, gmbh, hamburg, west germany) was studied in 12-week old male wistar rats on the following: thyroid weights and morphology, t3-t4 serum levels, thyroid lactic dehydrogenase, atp-ase, acid phosphatase, and non-specific esterase activities. thymus extract was administered intramuscularly daily for 21 days at doses of 0.5, 1.0 and 2.0 ml/kg. measurements were made on day 3, 7, 14 and 21 of treatment. thyroid histology and enzyme a ... | 1984 | 6235526 |
| expression of myosin isoenzymes in cardiac-muscle cells in culture. | myosin isoenzyme profiles of rat and chicken embryonic cardiac myocytes were studied during differentiation and growth in vitro by native-gel electrophoresis and assay of ca2+-activated atpase. the electrophoretic pattern of myosin extracted from 18-day-embryonic-rat myocytes after 7 days in culture exhibits three isoenzyme bands, v1, v2 and v3, of which the slow-migrating v3 is predominant. this resembles the isoenzyme profiles from 18-20-day-embryonic ventricles in vivo. however, the isoenzyme ... | 1984 | 6235805 |
| analysis of lymphocyte subsets in patients with aplastic anaemia. | lymphocyte subsets have been measured in the blood of 28 patients with aplastic anaemia. the mean helper/inducer:suppressor/cytotoxic t lymphocyte ratio (1.24 +/- 0.74) was significantly decreased in the total population in comparison to mean ratios in a normal population (1.78 +/- 0.57) and in patients with other haematological diseases (1.82 +/- 0.92). a reversed ratio (less than or equal to 1) was present in a large proportion (53%) of aplastic patients, due both to an absolute deficiency of ... | 1984 | 6235836 |
| [development of young rabbits exposed to an intense electric field]. | newborn rabbits were exposed 18 hours a day for 6 weeks to an intense electric field; then they were killed, the body weight and the weights of the main organs had the same values as the control ones. the compositions of liver and interscapular brown fat were not modified. there was no significant effect of the electric field either on plasma levels of acth, gh, t3, t4, corticosterone, cortisol or on plasma levels of glucose, triglycerides and cholesterol. the histology of thyroid and adrenals s ... | 1984 | 6235896 |
| [mechanisms of the effect of exogenous thyroxine on the regulation of the immune response in mice]. | the influence of repeated administration of thyroxin (t4) in doses of 4 or 4000 micrograms/100 g body weight daily upon t-b co-operation and specific t-suppressor formation was studied on cba male rats. a stimulating effect of t4 low doses on the lymph node t-cell ability was demonstrated to co-operate with the normal marrow b-cells over a process of generation of the humoral response to the sheep red blood cells within the system of singeneic transfer. the injection of various t4 doses to donor ... | 1984 | 6236457 |
| human b-cell-stimulatory-factor production by both t4+ and t8+ lymphocytes. | the t-cell subsets responsible for the production of human b-cell-stimulatory factor (bsf) have been identified. peripheral blood mononuclear cells (pbmnc), e-rosette-forming cells, and isolated t4+/t8+ subpopulations were cultured for 5 days with and without phytohemagglutinin (pha) stimulation; the supernatants were then assayed for bsf. bsf activity was detected by a costimulation assay using either staphylococcus aureus cowan i (sac) or goat anti-human igm as a b-cell comitogen. supernatants ... | 1984 | 6236886 |
| effects of prostaglandin e2 on responses of t-cell subsets to mitogen and autologous non-t-cell stimulation. | the cellular basis for prostaglandin e2 (pge2)-mediated suppression of human t-cell proliferative responses was examined. proliferation in response to concanavalin a was found to be more sensitive to the suppressive influence of pge2 than was that to phytohemagglutinin. studies of separated t cells indicated that t4+ cells were inhibited to a greater extent than were t8+ cells. addition of interleukin 2 (il-2) to the cultures did not overcome the suppression. in studies conducted in the autologo ... | 1984 | 6236922 |
| peripheral blood t-cell subsets studied by monoclonal antibodies in type 1 (insulin-dependent) diabetes: effect of blood glucose control. | peripheral t lymphocyte subsets were investigated, using monoclonal antibodies, in 14 patients with acute diabetes of duration less than 1 month (before insulin treatment) and after prolonged strict blood glucose control, and also in 40 healthy volunteers. at the time of diagnosis, the percentage total t cells was decreased (67.6 +/- 8.4 versus 72.8 +/- 6.6%), but t4 'helper' cells and t8 'suppressor/cytotoxic' cells were in the normal range. the t4/t8 ratio was not significantly higher than in ... | 1984 | 6237013 |
| lymphocyte sub-population counts after a single 40 mg administration of piroxicam in 20 patients with rheumatoid arthritis. a placebo-controlled study. | patients with active rheumatoid arthritis are characterized by a decrease in the number of circulating t-suppressor lymphocytes (identified by okt8), resulting in an imbalance between helper (identified by okt4) and suppressor cells. piroxicam is a non-steroidal anti-inflammatory agent which modulates lymphocytic functions, especially by reducing the concentration of the rheumatoid factor in vitro and in vivo. a double-blind placebo-controlled study was performed in 20 patients suffering from ac ... | 1984 | 6237051 |
| natural killer cell function and modulation by alpha ifn and il2 in aids patients and prodromal subjects. | we have examined the primary immune response, the numbers of the total t (t-11+) cells, t-helper (t4+) cells, t-suppressor (t8+) cells, nk cells (leu7+) and the level of serum igg in 83 healthy male heterosexuals and compared the data to those obtained from 12 clinically diagnosed aids patients and 92 asymptomatic male homosexuals. the absolute number of total t (t11) and t helper (t4) cell populations were significantly reduced among most of the asymptomatic homosexual males and even more decre ... | 1984 | 6237206 |
| [autologous mixed lymphocyte culture (amlc)]. | the autologous mixed lymphocyte culture (amlc) in humans has been reviewed. normal human t cells are stimulated to proliferate in vitro when co-cultured with mitomycin-c-treated or x-irradiated autologous non-t-cells. this reactivity, termed autologous mixed lymphocyte reaction, is thought to represent a self-recognitive mechanism that might be important in regulating the cellular interactions involved in the generation of normal immune response. the nature of the stimulator cell remains a matte ... | 1984 | 6237558 |
| lymphokine production and lymphocyte subpopulations in patients with head and neck squamous carcinoma. | prior studies of impaired cellular immune reactivity in patients with head and neck squamous carcinoma (hnsc) suggest that immune deficiency associated with tumor growth may be related, in part, to alterations in immunoregulatory functions. to determine if production of soluble mediators of the immune response (lymphokines) is impaired in patients with hnsc, leukocyte migration inhibition in response to phytohemagglutinin was assessed in 32 patients with hnsc and 29 normal subjects and was corre ... | 1984 | 6237633 |
| growth, interleukin-2 production, and responsiveness to il-2 in t4-positive t lymphocyte populations from malignant cutaneous t cell lymphoma (sézary's syndrome): the effect of cyclosporin a. | functional analysis and surface phenotyping using monoclonal antibodies have revealed that malignant t lymphocyte populations in the peripheral blood of patients with sézary's syndrome resemble the t helper cell populations from normal individuals. in this article we have studied the effects of the immunosuppressive drug cyclosporine a (csa) on growth, interleukin-2 (il-2) production, and the induction of il-2 responsiveness of peripheral blood monocytes (pbms) from five patients with sézary's s ... | 1984 | 6237693 |
| production of ige-potentiating factor in man by t cell lines bearing fc receptors for ige. | the regulation of human ige production in vitro by soluble t cell factors was examined. t cells were isolated from the peripheral blood of 2 patients with the hyper-ige syndrome on the basis of their expression of fc receptors for human ige (fc epsilon r). the t cells were incubated with human myeloma ige (10 micrograms/ml), washed, reacted with immunosorbent-purified goat anti-human ige conjugated with fluorescein isothiocyanate, and then separated into fc epsilon r+ and fc epsilon r- t cells o ... | 1984 | 6237918 |
| a comparative study of streptococcal antigen-binding human t8+ cells and monocytes, in relation to the hla-drw6 locus and the helper, suppressor and contrasuppressor functions. | binding of streptococcal antigen was compared between t8+ cells and monocytes (mo) from drw6+ and drw6- subjects. both t8+ cells and mo from drw6- subjects bind streptococcal antigen (sa) specifically and almost entirely at a concentration of 1000 ng per 0.1 ml. however, the drw6+, t8+ cells bind sa at a concentration of 1 ng but the drw6+ mo bind sa at both 1 and 1000 ng of sa per 0.1 ml. the hla-dr-dependent shift in the binding concentration of sa applied to mo as well as t8+ cells, except th ... | 1984 | 6237980 |
| prevalence of antibodies to lymphadenopathy-associated retrovirus in african patients with aids. | the presence of antibodies to lymphadenopathy-associated retrovirus (lav) was determined by a radioimmunoprecipitation assay and by an enzyme-linked immunosorbent solid assay of sera from zairian patients with the acquired immune deficiency syndrome (aids) in 1983. thirty-five of 37 patients (94 percent) and 32 of 36 patients (88 percent), respectively, were seropositive by the two tests. in a control group of 26 patients, six (23 percent) showed positive results in these tests. of these six con ... | 1984 | 6238406 |
| nonsteroidal anti-inflammatory drugs and immunologic function. overview of the european experience. | a large number of nonsteroidal anti-inflammatory drugs are available in europe. there appears to be little difference in overall clinical effectiveness of these drugs, despite individual patient variation, suggesting a common mode of action. occasionally, a nonsteroidal anti-inflammatory drug will show some evidence of a more impressive anti-rheumatic effect, perhaps related to a more prolonged or different mode of action. the evidence for an immunologic effect of nonsteroidal anti-inflammatory ... | 1984 | 6238529 |
| regulation of hepatic triiodothyronine production in the streptozotocin-induced diabetic rat. | the effect of diabetes on 3,5,3'-triiodothyronine (t3) production was determined in the isolated perfused rat liver. induction of diabetes with streptozotocin resulted in decreased serum thyroxine (t4) and t3 levels and a progressive decline in hepatic t3 production over 5 days. the decline in t3 production resulted from decreased conversion of t4 to t3, whereas t4 uptake was unchanged. insulin administration restored serum t4 and t3, hepatic conversion of t4 to t3, and t3 production to normal l ... | 1984 | 6238537 |
| lymphocyte subpopulations in patients with allergic rhinitis. | the lymphocyte subpopulations were classified using monoclonal antibodies specific for b lymphocytes (b1 antibodies), t lymphocytes (t11 and okt3 antibodies), helper/inducer t cells (t4 antibodies) and suppressor/cytotoxic t cells (t8 antibodies). three groups of subjects were studied: 20 normal controls, 29 patients with allergic rhinitis and a subgroup of nine patients who had received immunotherapy. the proportion of b lymphocytes, total t cells and t4 positive (helper/inducer) cells were not ... | 1984 | 6238546 |
| deficient helper cell function as a cause of diminished pokeweed mitogen blastogenic responses in patients with non-hodgkin's lymphomas. | an investigation has been made of immunoregulatory t-cell function in the non-hodgkin's lymphomas by comparing immunoregulation of healthy control and patient peripheral blood lymphocyte blastogenic responses to pokeweed mitogen. normal mononuclear leukocytes (mnl) had significantly higher responses than patient mnl. mnl were subsequently separated into t- and non-t-cell fractions by differential e-rosette sedimentation for co-culture experiments. when normal non-t-cells and autologous irradiate ... | 1984 | 6238671 |
| on the fidelity of dna replication. the accuracy of t4 dna polymerases in copying phi x174 dna in vitro. | the fidelity with which wild type t4 dna polymerase copies phi x174 amber 3 plus strand dna at position 587 in vitro has been measured. synthesis is initiated by hybridizing to the template a haeiii restriction fragment whose 3'-oh terminus is 83 nucleotides from the amber 3 site. based on gel electrophoresis of product dna molecules and genetic marker rescue data, t4 dna polymerase copies significantly beyond the mutant site. transfection analysis shows that the a x t leads to g x c mutation at ... | 1984 | 6229537 |
| a family with hereditary lack of t4+ inducer/helper t cell subsets in peripheral blood lymphocytes. | 1984 | 6229573 | |
| regulatory interactions governing the proliferation of t cell subsets stimulated with pokeweed mitogen. | serial phenotyping of human peripheral blood mononuclear cells (pbmc) cultured with pokeweed mitogen (pwm) demonstrated an excess of t8+ cells after stimulation. preferential expansion of the t8+ cell compartment was a result of t8+ cell blast transformation while t4+ cells generated fewer blasts and tended to remain as small resting cells. when the proliferative behavior of t cell subsets in pwm-stimulated pbmc with physiologic proportions of t4+ and t8+ cells was compared with that of cultures ... | 1984 | 6229576 |
| [fruste form of hyperthyroidism manifested by auricular arrhythmia. importance of the assay of the free fraction of thyroxine (ft4) and the role of the trh test]. | the trh test was used to detect hyperthyroidism in 87 patients aged from 38 to 85 years who presented with atrial arrhythmia with or without heart disease. the patients had no clinical evidence of thyrotoxicosis, and total thyroxine (t4), free thyroxine index (fti) and triiodothyronine (t3) values were normal. hyperthyroidism was diagnosed in the 18 patients (21%) with negative trh test; 15 of them had high free thyroxine (ft4) levels. the most common causes of hyperthyroidism were "warm" nodule ... | 1984 | 6229741 |
| [assay of the free fraction of thyroxine. its role in the study of thyroid function]. | in a prospective study of 467 subjects with normal, increased or decreased thyroid function the results of free thyroxine (ft4) assays by 2 radioimmunological methods were compared with the free thyroxine index (ft1) and the t4/tbg ratio. it appeared from this study that ft4 assays were at least as good as the ft1 to confirm the diagnosis of euthyroidism and were distinctly superior to measurements of ft1, t4 adn t3 in patients with abnormal thyroid function. in view of the close correlation obs ... | 1984 | 6229759 |
| regulation of normal human blood neutrophilic, macrophagic, and eosinophilic committed stem cell proliferation by autologous blood t lymphocyte subsets. | the influence of t lymphocyte (t cell) subsets on the proliferation of the blood neutrophilic, macrophagic, and eosinophilic cluster and colony (aggregate) forming cells (cfc) was evaluated. the t cells and the cfc-enriched b-null cells (mononuclear cells depleted of monocytes and t cells) and null cells (b-null) cells depleted of b lymphocytes) were separated from the blood of normal individuals. the population enriched for okt4 (t4 cells) monoclonal antibody-binding t helper/inducer cells and ... | 1984 | 6229296 |
| dissociation of helper/inducer t-cell functions: immunodeficiency associated with mycobacterial histiocytosis. | mycobacterial histiocytosis is a generally fatal disease characterized by abundant amounts of undigested atypical mycobacteria present within tissue macrophages that diffusely infiltrate the affected lymph nodes. the immune functions of a 22-year-old man with infection due to mycobacterium avium were investigated. although the patient had a marked increase in serum immunoglobulin levels and detectable antibodies toward mycobacterial antigens, he had no cellular response to these antigens both in ... | 1984 | 6229377 |
| vasoactive intestinal peptide stimulation of human thyroid cell function. | neurons containing vasoactive intestinal peptide (vip) have been found to reach thyroid follicular cells in man and other mammals. human thyroid cells in culture were exposed to different concentrations of vip and tsh, and thyroid cell activation was assessed by measurements of camp accumulation. vip exerted a dose-dependent activation, and this thyroid-stimulating effect was more rapid and short-acting than that of tsh. at low concentrations of the two agents, their effects were additive, but t ... | 1984 | 6315758 |
| the effect of streptococcus pneumoniae infection on the binding of thyroxine to purified rat liver plasma membranes. | hepatic plasma membranes were isolated from control and streptococcus pneumoniae-infected rats in order to determine the effect of s. pneumoniae infection on the binding capacity and affinity of hepatic plasma membranes for t4. infection did not affect the purity or the yield of isolated membranes. a significant decrease in both total and free serum t4 was observed during infection. scatchard analysis of membrane binding, determined under optimal conditions, confirmed the presence of high affini ... | 1984 | 6317353 |
| ontogenesis of the nuclear 3,5,3'-triiodothyronine receptor in the human fetal brain. | a high-affinity t3 binding site with the binding specificity of the nuclear t3 receptor is present in the brain of the human fetus at midgestation. its concentration was found to be very low at 10 weeks of gestation, and increased by a factor of 10 up to the 16th week, in coincidence with the period of neuroblast multiplication. liver, heart, and lung also contained receptor. both t4 and t3 were present in the brain, as measured by ria. in other tissues, however, only t4 was detected, suggesting ... | 1984 | 6317365 |
| a hotspot for transition mutations in the riib gene of bacteriophage t4. i. the extent of the hotspot. | we have previously demonstrated that the sequence 5'tggcaa 3' located at codons 32-33 of the riib gene of bacteriophage t4 is a hotspot for transition mutations (nelson et al. 1981). here i report the properties of the same tggcaa sequence introduced into the gene at codons 11-12. the sequence is highly mutable in both locations, suggesting that its high mutability is due to features of the tggcaa itself and is not dependent on the immediate juxtaposition of additional external sequences. within ... | 1984 | 6318044 |
| nih conference. acquired immunodeficiency syndrome: epidemiologic, clinical, immunologic, and therapeutic considerations. | the acquired immunodeficiency syndrome is a new disease whose cause is unknown but is almost surely due to a transmissible agent, most likely a virus. the disease is clearly spread by sexual contact, particularly homosexual activity. blood-borne transmission constitutes the other major recognized form of spread of the disease, although it is highly likely that the disease is not readily spread through casual, nonsexual, non-blood-borne routes. although the disease is still highly concentrated in ... | 1984 | 6318629 |
| cruciform-resolvase interactions in supercoiled dna. | t4 endonuclease vii, which cleaves holliday-like junctions in dna, specifically cleaves short inverted repeats in supercoiled plasmids. these sequences are subject to site-specific cleavage by single-strand-specific nucleases, and cruciform formation has been suggested as an explanation for this observation. this proposal is greatly strengthened by the present data, since a formal analogy between cruciform structures and holliday junctions exists. resolution of a variety of unrelated cruciform s ... | 1984 | 6319022 |
| cloning and physical mapping of an early region of the bacteriophage t4 genome. | we have cloned dna restriction fragments from the largely nonessential region of bacteriophage t4 located between genes 39 and 56. the cloned dna fragments were used to construct a precise map of the sites in this region recognized by eight restriction endonucleases. this restriction map allowed us to compare the cytosine-containing t4 dna used for cloning with the hydroxymethylcytosine-containing dna of wild-type t4; there were no detectable rearrangements in the region tested. we were also abl ... | 1984 | 6319228 |
| leydig cell hypoplasia causing male pseudohermaphroditism: diagnosis 13 years after prepubertal castration. | an 11-yr-old patient with male pseudohermaphroditism who was castrated at nine days of age and raised thereafter as a female was evaluated to determine the cause of abnormal sexual differentiation. stimulation with acth for 8 h revealed no abnormality in the biosynthesis of cortisol or adrenal androgens. the administration of fluoxymesterone (10 mg, orally, daily) for 5 weeks at age 13 yr led to significant decrements in serum levels of sex steroid-binding globulin and t4-binding globulin to a d ... | 1984 | 6319451 |
| characterization of guinea pig cytomegalovirus dna. | the genome of guinea pig cytomegalovirus (gpcmv) was analyzed and compared with that of human cytomegalovirus (hcmv). gpcmv and hcmv dnas were isolated from virions and further purified by cscl centrifugation. purified gpcmv dna sedimented as a single peak in a neutral sucrose gradient and was infectious when transfected into guinea pig embryo fibroblast cells. the cytopathology was characteristic of that seen after infection with gpcmv. virus dna purified from virions isolated from infected gpe ... | 1984 | 6319742 |
| nuclear thyroxine and 3,5,3'-triiodothyronine receptors in human mononuclear blood cells during pregnancy. | serum free t4, free t3, tsh and maximal nuclear binding capacity for t4 and t3 in mononuclear blood cells were measured in 12 control women, in 12 normal pregnant women in the first and in 12 women in the third trimester. serum free t4 and t3 were decreased in late pregnancy compared to control women, serum free t4: 9.1 pmol/l(mean) vs 12.9 pmol/l(mean); serum free t3: 4.0 pmol/l(mean) vs 6.2 pmol/l(mean), without any change in tsh levels: 2.2 mu/l(mean) vs 1.8 mu/l (mean). concomitantly, the ma ... | 1984 | 6320566 |
| putative nuclear triiodothyronine receptors in tadpole erythrocytes: regulation of receptor number by thyroid hormone. | putative thyroid hormone (th) receptors have been detected in the nuclei of red blood cells (rbcs) from rana catesbeiana tadpoles, and their binding characteristics have been examined. nuclear t3 saturation analyses were carried out in vitro in intact rbcs suspended in phosphate-buffered amphibian ringer. after incubation with t3, intact nuclei were obtained by centrifugation after lysing the cells in a sucrose-tris-hcl buffer containing 0.2% saponin and then adding triton x-100 (final concentra ... | 1984 | 6321136 |
| okt4+ cytotoxic t cells can lyse targets via class i molecules and can be blocked by monoclonal antibody against t4 molecules. | human cytotoxic t lymphocytes (ctl) have been shown to recognize either class i or class ii major histocompatibility (mhc) products. this recognition has been correlated with the expression of okt antigens on the surface of the ctl. thus, okt4+ ctl have been shown to be reactive with class ii products, whereas okt8+ effectors recognize class i molecules. in this study, responder cells were separated according to their okt4 or okt8 cell surface phenotype on a fluorescence-activated cell sorter (f ... | 1984 | 6332131 |
| immunoregulation in an isolated 12-year-old boy with congenital severe combined immunodeficiency. | we report the evaluation of in vitro immunoregulation in a 12-year-old untreated boy with severe combined immunodeficiency (scid). severely hypogammaglobulinemic, the patient was incapable of a specific antibody response to either natural substances or administered antigens. ficoll-hypaque-isolated peripheral blood mononuclear cells (mnl) from the patient failed to respond to pokeweed mitogen (pwm) with the normal increment in immunoglobulin-secreting cells, as measured by a reverse hemolytic pl ... | 1984 | 6332299 |