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how important is vertical transmission of dengue viruses by mosquitoes (diptera: culicidae)?vertical transmission of dengue viruses by mosquitoes was discovered at the end of the late 1970s and has been suggested to be a means by which these viruses persist. however, it is unclear how widespread it is in nature, and its importance in the epidemiology of this disease is still debated. here, we review the literature on vertical transmission and discuss its role in dengue's epidemiology and control. we conclude that given the number of studies that failed to find evidence of vertical tran ...201626545718
satyrization and satyrization-resistance in competitive displacements of invasive mosquito species.competitive displacements or reductions of resident populations of insects, often effected by a related species, may be caused by a variety of mechanisms. satyrization is a form of mating interference in which males of one species mate with females of another species, significantly decreasing their fitness and not generating hybrids. satyrization has been established to be the probable cause of competitive displacements of resident mosquitoes by invasive species, especially of aedes aegypti by a ...201626542083
plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review.mosquitoes (diptera: culicidae) are a key threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. mosquito young instars are usually targeted with organophosphates, insect growth regulators and microbial control agents. indoors residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have strong negative effects on human health and the environment. newer and safer tools have been recently implemented to enh ...201626541154
nepenthes ampullaria (nepenthaceae) pitchers are unattractive to gravid aedes aegypti and aedes albopictus (diptera: culicidae).nepenthes pitcher plants are colonized by a variety of specialized arthropods. as aedes mosquitoes are container breeders, nepenthes pitchers are a potential candidate oviposition site for vector species, such as aedes aegypti (l.) and aedes albopictus (skuse). however, aedes spp. are not commonly encountered in nepenthes pitchers, and the environment inside the pitchers of some species is lethal to them. one exception is nepenthes ampullaria jack, whose pitchers are known to be colonized by ae. ...201626518035
mosquito salivary allergen aed a 3: cloning, comprehensive molecular analysis, and clinical evaluation.allergic reactions to mosquito bites are an increasing clinical concern. due to the lack of availability of mosquito salivary allergens, they are underdiagnosed. here, we reported a newly cloned mosquito aedes (ae.) aegypti salivary allergen.201626608594
a novel in vitro bioassay to explore the repellent effects of compounds against mosquito aedes aegypti (diptera: culicidae).mosquitoes are vectors for many pathogens resulting in many deaths of humans. repellents play an important role in reducing mosquito bites and the spread of mosquito-borne diseases. currently, klun & debboun (k & d) and human-arm-based bioassay systems are used to identify repellent properties of compounds, extracts, and essential oils. risks involved with human-arm-based systems are allergic reactions and limited replicates. we are reporting an in vitro bioassay method “ncnpr repellent bioassay ...201626590191
the siren's song: exploitation of female flight tones to passively capture male aedes aegypti (diptera: culicidae).the need to capture male mosquitoes has intensified recently as a result of a number of male-based sterile insect technique (sit) and population-modification programs focused on aedes aegypti (l.) having initiated field releases. here, we report the results of the successful exploitation of the attraction of male ae. aegypti to female flight tones to enhance male collections in nonmechanical passive (nonbattery powered) gravid aedes traps (gat). prior to field studies, male attraction to female ...201626502754
the influence of ambient temperature on the susceptibility of aedes aegypti (diptera: culicidae) to the pyrethroid insecticide permethrin.insecticides are the most common strategy used for the management of mosquitoes. changes in ambient temperature can alter the toxicity of insecticides to ectothermic organisms. studies show organophosphate insecticides exhibit a positive correlation between ambient temperature and mortality for many insect species, and carbamate insecticides exhibit a slightly negative correlation between ambient temperature and mortality. pyrethroid insecticides exhibit a distinctly negative correlation between ...201626477050
improving the effectiveness of three essential oils against aedes aegypti (linn.) and anopheles dirus (peyton and harrison).repellency of essential oil extracted from curcuma longa, eucalyptus globulus, and citrus aurantium at various concentrations (5, 10, 15, 20, and 25 %) with and without 5 % vanillin was evaluated against female mosquitoes: aedes aegypti and anopheles dirus. the comparisons were made with a commercial chemical repellent (n,n-diethyl-3-methylbenzamide (deet) 25 % w/w; kor yor 15) by arm in cage method. it was found that the essential oils with 5 % vanillin gave the longest lasting period against t ...201626358103
first report of leptolegnia chapmanii (peronosporomycetes: saprolegniales) affecting mosquitoes in central brazil.numerous isolates of an oomycete 'fungus', leptolegnia chapmanii, are reported from brazil for the first time. this aquatic pathogen was baited with aedes aegypti sentinel larvae from stagnant, temporary bodies of water in selected locations under secondary tropical forest in and near the central brazilian city of goiânia and from more distant sites in the western and northern regions of the state of goiás. isolates were identified based on their morphological and developmental characters, compa ...201627018147
the response of claudin-like transmembrane septate junction proteins to altered environmental ion levels in the larval mosquito aedes aegypti.septate junctions (sjs) occlude the paracellular pathway and function as paracellular diffusion barriers within invertebrate epithelia. however, integral components of sjs and their contribution to barrier properties have received considerably less attention than those of vertebrate occluding junctions. in arthropods, sj proteins have only been identified in drosophila and among these are three integral claudin-like proteins, megatrachea (mega), sinuous (sinu) and kune-kune (kune), as well as a ...201627004691
ecdysis period and rate deviations of dengue mosquito vector, aedes aegypti reared in different artificial water-holding containers.artificial water-holding containers (awhcs) have been well-documented in many aedes aegypti studies for dengue surveillance and developmental research. hence, we investigated the role of different ahwcs on the development and ecdysis period of ae. aegypti dengue vector, a container breeding mosquito.201627004577
mosquito adulticidal activity of the leaf extracts of spondias mombin l. against aedes aegypti l. and isolation of active principles.aedes aegypti is a domestic mosquito and one of the primary vectors for dengue and yellow fever. since, it is a vector of deadly diseases, its control becomes essential. medicinal plants may be an alternative to adulticidal agents since they contain rich source of bioactive compounds. this study was designed to determine the adulticidal activity of spondias mombin leaf methanol crude extract, n-hexane, dichloromethane and ethyl acetate fractions against female adults of ae. aegypti mosquitoes an ...201627004574
plasmodium delichoni n. sp.: description, molecular characterisation and remarks on the exoerythrocytic merogony, persistence, vectors and transmission.malaria parasite plasmodium (novyella) delichoni n. sp. (haemosporida, plasmodiidae) was found in a widespread eurasian songbird, the common house martin delichon urbicum (hirundinidae). it is described based on the morphology of its blood stages and segments of the mitochondrial cytochrome b and apicoplast genes, which can be used for molecular identification of this species. erythrocytic meronts and gametocytes are strictly nucleophilic, and mature gametocytes possess pigment granules of marke ...201627000087
incremental cost of implementing residual insecticide treatment with delthametrine on top of intensive routine aedes aegypti control.information on the cost of implementing residual insecticide treatment (rit) for aedes control is scarce. we evaluated the incremental cost on top of intensive conventional routine activities of the aedes control programme (acp) in the city of santiago de cuba, cuba.201626996279
chemical composition, toxicity and non-target effects of pinus kesiya essential oil: an eco-friendly and novel larvicide against malaria, dengue and lymphatic filariasis mosquito vectors.mosquitoes (diptera: culicidae) are vectors of important parasites and pathogens causing death, poverty and social disability worldwide, with special reference to tropical and subtropical countries. the overuse of synthetic insecticides to control mosquito vectors lead to resistance, adverse environmental effects and high operational costs. therefore, the development of eco-friendly control tools is an important public health challenge. in this study, the mosquito larvicidal activity of pinus ke ...201626995063
composition and biological activities of the essential oil of piper corcovadensis (miq.) c. dc (piperaceae).essential oil from fresh leaves of the shrub piper corcovadensis (miq.) c. dc was obtained in 0.21% (w/w) yield by hydrodistillation in a clevenger type apparatus. thirty-one components, accounting for 96.61% of the leaf oil, were identified by gas chromatography-mass spectrometry. the major constituents of the oil were 1-butyl-3,4-methylenedioxybenzene (30.62%), terpinolene (17.44%), trans -caryophyllene (6.27%), α-pinene (5.92%), δ-cadinene (4.92%), and limonene (4.46%). bioassays against larv ...201626993082
dna barcoding and wing morphometrics to distinguish three aedes vectors in thailand.aedes aegypti (diptera: culicidae) (l.), ae. albopictus (skuse), and ae. scutellaris (walker) are important mosquito vectors of dengue and chikungunya viruses. they are morphologically similar and sympatric in some parts of their distribution; therefore, there is a risk of incorrect morphological identification. any confusion could have a negative impact on epidemiological studies or control strategies. therefore, we explored two modern tools to supplement current morphological identification: d ...201626987285
effect of two commercial herbicides on life history traits of a human disease vector, aedes aegypti, in the laboratory setting.some mosquito species utilize the small niches of water that are abundant in farmland habitats. these niches are susceptible to effects from agricultural pesticides, many of which are applied aerially over large tracts of land. one principal form of weed control in agricultural systems involves the development of herbicide-tolerant crops. the impact of sub-agricultural levels of these herbicides on mosquito survival and life-history traits of resulting adults have not been determined. the aim of ...201626965703
an animal homolog of plant mep/amt transporters promotes ammonia excretion by the anal papillae of the disease vector mosquito aedes aegypti.the transcripts of three putative ammonia (nh3/nh4 (+)) transporters, rhesus-like glycoproteins aerh50-1, aerh50-2 and amt/mep-like aeamt1 were detected in the anal papillae of larval aedes aegypti quantitative pcr studies revealed 12-fold higher transcript levels of aeamt1 in anal papillae relative to aerh50-1, and levels of aerh50-2 were even lower. immunoblotting revealed aeamt1 in anal papillae as a pre-protein with putative monomeric and trimeric forms. aeamt1 was immunolocalized to the bas ...201626944496
aedes aegypti control in brazil. 201626944024
deltamethrin-mediated survival, behavior, and oenocyte morphology of insecticide-susceptible and resistant yellow fever mosquitos (aedes aegypti).insecticide use is the prevailing control tactic for the mosquito aedes aegypti, a vector of several human viruses, which leads to ever-increasing problems of insecticide resistance in populations of this insect pest species. the underlying mechanisms of insecticide resistance may be linked to the metabolism of insecticides by various cells, including oenocytes. oenocytes are ectodermal cells responsible for lipid metabolism and detoxification. the goal of this study was to evaluate the subletha ...201626943998
[dengue, zika and chikungunya].arboviruses are transmitted by arthropods, including those responsible for the current pandemic: alphavirus (chikungunya) and flaviviruses (dengue and zika). its importance increased in the americas over the past 20 years. the main vectors are aedes aegypti and a. albopictus. dengue infection provides long lasting immunity against the specific serotype and temporary to the other three. subsequent infection by another serotype determines more serious disease. there is a registered vaccine for den ...201626942903
repellent and mosquitocidal effects of leaf extracts of clausena anisata against the aedes aegypti mosquito (diptera: culicidae).mosquitoes are rapidly developing resistance to insecticides that millions of people relied on to protect themselves from the diseases they carry, thereby creating a need to develop new insecticides. clausena anisata is used traditionally as an insect repellent by various communities in africa and asia. for this study, the repellency and adulticidal activities of leaf extracts and compounds isolated from this plant species were evaluated against the yellow fever mosquito, aedes aegypti. in the t ...201626924698
structural insights into the aedes aegypti aquaporins and aquaglyceroporins - an in silico study.there has been a fair bit of understanding on the structure-function relationship of aquaporins (aqps) from plants and vertebrates obtained from available x-ray crystallography data. however, there is a lacuna in understanding the structure of aqps from sanguinivorous insects like the mosquito where it plays a crucial role in survival. in this study, we have built homology models for the aedes aegypti aqps, identified key channel lining residues and compared the structure and sequence with ortho ...201626906718
control of mosquito-borne infectious diseases: sex and gene drive.sterile male releases have successfully reduced local populations of the dengue vector, aedes aegypti, but challenges remain in scale and in separating sexes before release. the recent discovery of the first mosquito male determining factor (m factor) will facilitate our understanding of the genetic programs that initiate sexual development in mosquitoes. manipulation of the m factor and possible intermediary factors may result in female-to-male conversion or female killing, enabling efficient s ...201626897660
spatial spread of dengue in a non-endemic tropical city in northern argentina.after more than eighty years dengue reemerged in argentina in 1997. since then, the largest epidemic in terms of geographical extent, magnitude and mortality, was recorded in 2009. in this report we analyzed the den-1 epidemic spread in orán, a mid-size city in a non-endemic tropical area in northern argentina, and its correlation with demographic and socioeconomic factors. cases were diagnosed by elisa between january and june 2009. we applied a space-time and spatial scan statistic under a poi ...201626875764
discovery of selective inhibitors targeting acetylcholinesterase 1 from disease-transmitting mosquitoes.vector control of disease-transmitting mosquitoes is increasingly important due to the re-emergence and spread of infections such as malaria and dengue. we have conducted a high throughput screen (hts) of 17,500 compounds for inhibition of the essential ache1 enzymes from the mosquitoes anopheles gambiae and aedes aegypti. in a differential hts analysis including the human ache, several structurally diverse, potent, and selective noncovalent ache1 inhibitors were discovered. for example, a pheno ...201627598521
proteomic analysis of aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets.in this work we analyzed protein expression in the aedes aegypti midgut during the larval (fourth instar, l4), pupal, and adult stages [including newly emerged (ne), sugar-fed (sf) and blood-fed (bf) females]. two-dimensional electrophoresis showed 13 spots in the midgut of larvae, 95 in the midgut of pupae, 90 in the midgut of ne, and 76 in the midgut of sf or bf females. in the larval midguts, high serpin expression was noted, while in the pupae, protein abundance was lower than in the ne, sf, ...201627597118
egg hatching and survival of immature stages of aedes aegypti (diptera: culicidae) under natural temperature conditions during the cold season in buenos aires, argentina.in temperate regions, the seasonal dynamics of aedes aegypti (l.) (diptera: culicidae) is mainly influenced by temperature. it is assumed that, during the winter season, the population remains as eggs and that the development and population growth of surviving eggs begin during the following spring. the aim of the current study was to assess egg hatching of ae. aegypti during the winter in buenos aires city (argentina), and analyze the survival of immature stages. the experiments consisted of im ...201627576509
mosquito c-type lectins maintain gut microbiome homeostasis.the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ...201627572642
toxicity and larvicidal activity of podophyllum-based lignans against aedes aegypti (diptera: culicidae).aedes aegypti l. (diptera: culicidae) is a mosquito species that has adapted to urban environments and is the main vector of dengue viruses. because of the increasing incidence of dengue, a more environmentally acceptable insecticide needs to be found. natural products have been and continue to be an important source of leading compounds that can be modified in order to develop new drugs. the lignan family of natural products includes compounds with a diverse spectrum of biological activity. pod ...201627561675
eggs viability of aedes aegypti linnaeus (diptera, culicidae) under different environmental and storage conditions in manaus, amazonas, brazil.the viability of aedes aegypti eggs was assessed in the amazon region. the eggs were maintained under different conditions: indoors (insectarium) and outdoors (natural environment), as well as in different storage types (plastic cup, paper envelope, plastic bag) for different days. egg viability was measured as the mean of hatchings observed from egg-bearing sheets of filter paper immersed in water, using three sheets randomly selected from each storage type and at both sites. there were signifi ...201627533732
determination of juvenile hormone titers by means of lc-ms/ms/ms and a juvenile hormone-responsive gal4/uas system in aedes aegypti mosquitoes.in anautogenous mosquitoes, juvenile hormone iii (jh) plays an essential role in female post-eclosion (pe) development, preparing them for subsequent blood feeding and egg growth. we re-examined the jh titer during the reproductive cycle of female aedes aegypti mosquitoes. using liquid chromatography coupled with triple tandem mass spectrometry (lc-ms/ms/ms), we have shown that it reaches its peak at 48-54 h pe in the female hemolymph and at 72 h pe in whole body extracts. this method represents ...201627530057
aedes aegypti suppression in the americas: historical perspectives. 201627529102
phylogenetic analysis of the atp-binding cassette transporter family in three mosquito species.the atp-binding cassette (abc) transporter family functions in the atp-dependent transportation of various substrates across biological membranes. abc proteins participate in various biological processes and insecticide resistance in insects, and are divided into eight subfamilies (a-h). mosquitoes are important vectors of human diseases, but the mechanism by which the abc transporter family evolves in mosquitoes is unknown. in this study, we classified and compared the abc transporter families ...201627521922
solution nuclear magnetic resonance studies of sterol carrier protein 2 like 2 (scp2l2) reveal the insecticide specific structural characteristics of scp2 proteins in aedes aegypti mosquitoes.sterol carrier protein 2 like 2 from aedes aegypti (aescp2l2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. repeated unsuccessful attempts to crystallize ligand free scp2l2 prompted us to undertake nuclear magnetic resonance (nmr) spectroscopy to determine its three-dimensional structure. we report here the three-dimensional structures and dynamics of apo-aescp2l2 and its complex with palmitate. the (15)n heteronuclear single-quantum coherence spec ...201627508310
conidiobolus macrosporus (entomophthorales), a mosquito pathogen in central brazil.a new fungal pathogen of culicinae (diptera: culicidae) adults, conidiobolus macrosporus (entomophthorales: ancylistaceae), was detected and isolated during a survey of mosquito pathogens close to the city of aruanã, goiás state, in december 2014. the morphological characteristics of c. macrosporus are presented, and reasons for some uncertainty about this identification are discussed. the pathogenicity and high virulence of this fungus for aedes aegypti were confirmed in laboratory conditions. ...201627506454
identification of aacasps7, an effector caspase in aedes albopictus.aedes albopictus mosquito is a vector of various arboviruses and is becoming a significant threat to public health due to its rapid global expansion. several reports suggest that apoptosis could be a factor limiting arbovirus infection in mosquitoes. thus, it is significant to identify apoptosis pathway and study the correlation between apoptosis and virus infection in mosquitoes. apoptosis is a type of programmed cell death that plays a vital role in immunity, development, and tissue homeostasi ...201627502418
larvicidal activity of natural and modified triterpenoids against aedes aegypti (diptera: culicidae).insecticide resistance to commonly used substances demands new molecules for the chemical control of the dengue vector aedes aegypti. because natural product sources have been an alternative to obtain larvicidal compounds, the aim of this study was to evaluate the triterpenoids betulinic (ba) and ursolic (ua) acids and their semi-synthetic derivatives against larval ae. aegypti. ba, ua, ten derivatives modified at the c-3 position and a positive control (diflubenzuron) were evaluated. larvicidal ...201627501778
coexistence of aedes aegypti and aedes albopictus (diptera: culicidae) in peninsular florida two decades after competitive displacements.the spread of aedes albopictus (skuse) eastward in the mid-1980s from its initial establishment in houston, tx, was associated with rapid declines and local disappearances of aedes aegypti (l.) in gulf coast states and florida where annual larval surveillance during the early 1990s described temporal and spatial patterns of competitive displacements in cemeteries and tire shops. approximately 20 yr later in 2013-2014, we re-visited former collection sites and sampled aquatic immatures of these t ...201627493253
microrna-309 targets the homeobox gene six4 and controls ovarian development in the mosquito aedes aegypti.obligatory blood-triggered reproductive strategy is an evolutionary adaptation of mosquitoes for rapid egg development. it contributes to the vectorial capacity of these insects. therefore, understanding the molecular mechanisms underlying reproductive processes is of particular importance. here, we report that microrna-309 (mir-309) plays a critical role in mosquito reproduction. a spatiotemporal expression profile of mir-309 displayed its blood feeding-dependent onset and ovary-specific manife ...201627489347
the sex locus is tightly linked to factors conferring sex-specific lethal effects in the mosquito aedes aegypti.in many taxa, sex chromosomes are heteromorphic and largely non-recombining. evolutionary models predict that spread of recombination suppression on the y chromosome is fueled by the accumulation of sexually antagonistic alleles in close linkage to the sex determination region. however, empirical evidence for the existence of sexually antagonistic alleles is scarce. in the mosquito aedes aegypti, the sex-determining chromosomes are homomorphic. the region of suppressed recombination, which surro ...201627485667
ecotoxicological assessment of pyriproxyfen under environmentally realistic exposure conditions of integrated vector management for aedes aegypti control in brazil.there is increasing concern to control aedes aegypti mosquito exposure in developing countries such as brazil. thus, integrated approaches using a combination of chemical, pyriproxyfen larvicide, and biological, xiphophorus maculatus, larvivorous fish species approaches are necessary and important to initiate more effective control against mosquito borne diseases. this study describes the toxicological effects of pyriproxyfen larvicide on the fish xiphophorus maculatus, the larvivorous fish spec ...201627458879
comparative proteome analysis reveals that blood and sugar meals induce differential protein expression in aedes aegypti female heads.aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. during human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. here, we report changes in protein expression in the heads of adult female ae. aegypti mosquitoes in response to the ingestion of blood or sugar. proteins extracted from the heads of ae. aegypti fed exclusively on blood (bf) or sugar (sf) we ...201627343150
redeployment of a conserved gene regulatory network during aedes aegypti development.changes in gene regulatory networks (grns) underlie the evolution of morphological novelty and developmental system drift. the fruitfly drosophila melanogaster and the dengue and zika vector mosquito aedes aegypti have substantially similar nervous system morphology. nevertheless, they show significant divergence in a set of genes co-expressed in the midline of the drosophila central nervous system, including the master regulator single minded and downstream genes including short gastrulation, s ...201627341759
susceptibility to temephos and spinosad in aedes aegypti (diptera: culicidae) from puerto rico.we examined the susceptibility to temephos and spinosad (natular ec) of eight aedes aegypti (l.) populations from puerto rico, following who method (who 2005). enzyme activity was measured for alpha- and beta-esterases, multiple function oxidases, glutathione-s-transferases, and insensitive acetylcholinesterase and was tested for correlation with the susceptibility level. the results showed that larval populations from puerto rico obtained during 2014 were found to be susceptible to both larvici ...201627330095
structural characterization and evaluation of mosquito-larvicidal property of silver nanoparticles synthesized from the seaweed, turbinaria ornata (turner) j. agardh 1848.the silver nanoparticles synthesized from turbinaria ornata (to-agnps) showed spherical with crystalline nature (20-32 nm) was evaluated against fourth instar larvae of three mosquitoes. the maximum activity of to-agnps was recorded on aedes aegypti followed by anopheles stephensi and culex quinquefasciatus with the following lethal concentration values (μg/ml): lc50 of 0.738, 1.134, and 1.494; and lc90 of 3.342, 17.982, and 22.475, respectively. the obtained respective values (μg/ml) vis-a-vis ...201627327539
temporal frequency of knockdown resistance mutations, f1534c and v1016g, in aedes aegypti in chiang mai city, thailand and the impact of the mutations on the efficiency of thermal fogging spray with pyrethroids.in thailand, control of dengue outbreaks is currently attained by the use of space sprays, particularly thermal fogging using pyrethroids, with the aim of killing infected aedes mosquito vectors in epidemic areas. however, the principal dengue vector, aedes aegypti, is resistant to pyrethroids conferred mainly by mutations in the voltage-gated sodium channel gene, f1534c and v1016g, termed knockdown resistance (kdr). the objectives of this study were to determine the temporal frequencies of f153 ...201627325294
toxicological assessment of spinosad: implications for integrated control of aedes aegypti using larvicides and larvivorous fish.integration of larvivorous fish and biolarvicides at low concentrations to control of mosquito larvae in field situations may result in a safer and more effective tool. however, the usefulness of integrated approach depends upon survival and ecological fitness of fish employed. thus, the aim of this study was to examine the genotoxic effects of combining different sublethal concentrations of spinosad, a naturally occurring neurotoxic insecticide, with male adult poecilid larvivorous guppy (poeci ...201627294296
effects of preexposure to deet on the downstream blood-feeding behaviors of aedes aegypti (diptera: culicidae) mosquitoes.mosquito behavior is heavily influenced by the chemical molecules in the environment. this knowledge can be used to modify insect behaviors; particularly to reduce vector-host contact as a powerful method for disease prevention. n,n-diethyl-meta-toluamide (deet) is the most widely used insect repellent in the market and an excellent example of a chemical that has been used to modify insect behavior for disease prevention. however, genetic insensitivity and habituation in aedes aegypti (l.) mosqu ...201627288690
somatostatin signaling system as an ancestral mechanism: myoregulatory activity of an allatostatin-c peptide in hydra.the coordination of physiological processes requires precise communication between cells. cellular interactions allow cells to be functionally related, facilitating the maintaining of homeostasis. neuropeptides functioning as intercellular signals are widely distributed in metazoa. it is assumed that neuropeptides were the first intercellular transmitters, appearing early during the evolution. in cnidarians, neuropeptides are mainly involved in neurotransmission, acting directly or indirectly on ...201627288244
chemical composition and biological activity of essential oils from different species of piper from panama.the chemical composition of leaf essential oils from 11 species of piper from panama was analyzed by a combination gc-fid and gc-ms procedures. six of them had sesquiterpene hydrocarbons as major constituents, three were characterized by monoterpene hydrocarbons, one by a diterpene, and one by a phenylpropanoid, dillapiole. the main components identified in each species were: cembratrienol (25.4 %) in piper augustum; β-pinene (26.6 %) in piper corrugatum; α-pinene (19.4 %) in piper curtispicum; ...201627286333
reported distribution of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus in the united states, 1995-2016 (diptera: culicidae).aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) transmit arboviruses that are increasing threats to human health in the americas, particularly dengue, chikungunya, and zika viruses. epidemics of the associated arboviral diseases have been limited to south and central america, mexico, and the caribbean in the western hemisphere, with only minor localized outbreaks in the united states. nevertheless, accurate and up-to-date information for the geographical ranges of ae. aeg ...201627282817
semi-field evaluation of metofluthrin-impregnated nets on host-seeking aedes aegypti and anopheles dirus.the efficacy of a metofluthrin-impregnated net (min) known as the "mushikonazu" on the house entry behavior of female aedes aegypti and anopheles dirus mosquitoes was evaluated using a semi-field 50-m tunnel setup. while the min is labeled for the control of chironomids and moth flies, this study determined the feasibility of using the device, given its current construction and metofluthrin formulation, as a spatial repellent against mosquitoes. sentinel and cone bioassays were used to determine ...201627280351
new candidates for plant-based repellents against aedes aegypti.based on an ethnobotanical study on use for plant species against mosquito bites in the kota tinggi district, johor state, malaysia, 3 plants selected for study, citrus aurantifolia (leaves), citrus grandis (fruit peel), and alpinia galanga (rhizome), were extracted using hydrodistillation to produce essential oils. these essential oils were then formulated as a lotion using a microencapsulation process and then tested for their repellent effect against aedes aegypti. n,n-diethyl-m-toluamide (de ...201627280349
toxicology of potassium channel-directed compounds in mosquitoes.potential targets for new vector control insecticides are nerve and muscle potassium channels. in this study, the activities of known potassium channel blockers (4-aminopyridine, quinidine, and tetraethylammonium) and the insecticide propoxur were compared to three experimental catechols and several other compounds against anopheles gambiae and aedes aegypti mosquitoes. experimental catechol 1 was the most toxic experimental compound in all of the mortality assays conducted, but was at least 100 ...201627262624
evaluation of alternative killing agents for aedes aegypti (diptera: culicidae) in the gravid aedes trap (gat).the gravid aedes trap (gat) uses visual and olfactory cues to attract gravid aedes aegypti (l.) that are then captured when knocked down by a residual pyrethroid surface spray. however, the use of surface sprays can be compromised by poor availability of the spray and pesticide resistance in the target mosquito. we investigated several "alternative" insecticide and insecticide-free killing agents for use in the gat. this included long-lasting insecticide-impregnated nets (llins), vapor-active sy ...201627247350
species composition and habitat characterization of mosquito (diptera: culicidae) larvae in semi-urban areas of dhaka, bangladesh.mosquito larvae are purely aquatic and develop in water bodies, the type of which is more or less specific to each species. therefore, a study was carried out to identify the habitat characters of different mosquito species along with their species composition in semi-urban area of dhaka in bangladesh during the month of may and june 2012. a total of 6088 mosquito larvae belonging to 12 species (aedes aegypti, aedes albopictus, anopheles barbirostris, anopheles peditaeniatus, anopheles vagus, cu ...201627241953
effects of temperature on the life cycle, expansion, and dispersion of aedes aegypti (diptera: culicidae) in three cities in paraiba, brazil.the mosquito aedes aegypti is the primary vector of dengue and is common throughout tropical and subtropical regions. its distribution is modulated by environmental factors, such as temperature. this study aimed to evaluate the influence of temperature on the life cycle and expansion of ae. aegypti populations in the cities of campina grande, joão pessoa, and patos. samples of ae. aegypti were collected in the three cities and raised in the laboratory. we assessed the life cycles of the three ae ...201627232118
the effect of wolbachia on dengue outbreaks when dengue is repeatedly introduced.use of the wolbachia bacterium is a proposed new strategy to reduce dengue transmission, which results in around 390 million individuals infected annually. in places with strong variations in climatic conditions such as temperature and rainfall, dengue epidemics generally occur only at a certain time of the year. where dengue is not endemic, the time of year in which imported cases enter the population plays a crucial role in determining the likelihood of outbreak occurrence. we use a mathematic ...201627217229
yellow fever vaccination status and safety in hemodialysis patients.the adverse effects of yellow fever (yf) vaccine in dialysis patients are not well known. there is concern about the risks and benefits of the vaccine in immunocompromised patients living in endemic areas, particularly given the risk of resurgence of urban yf with the spread of aedes aegypti mosquitoes. the purpose of this study was to assess the coverage and safety of yf vaccine in chronic dialysis patients.201627208638
urban climate versus global climate change-what makes the difference for dengue?the expansion in the geographical distribution of vector-borne diseases is a much emphasized consequence of climate change, as are the consequences of urbanization for diseases that are already endemic, which may be even more important for public health. in this paper, we focus on dengue, the most widespread urban vector-borne disease. largely urban with a tropical/subtropical distribution and vectored by a domesticated mosquito, aedes aegypti, dengue poses a serious public health threat. temper ...201627197685
entomological assessment of yellow fever-epidemic risk indices in benue state, nigeria, 2010-2011.yellow fever (yf) is a vector-borne disease affecting humans and non-human primates in tropical areas. in the past, there have been pockets of yf outbreaks in nigeria that resulted in preventable deaths. surveillance efforts towards avoiding another outbreak have been put in place with the aim of early detection and control. however, risk indices relating to the density of immature yf-mosquito vectors are given little consideration even though it is the first step in curbing a possible outbreak. ...201627189925
functional and immunohistochemical characterization of cceae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors aedes aegypti and ae. albopictus.temephos is a major organophosphate (op) larvicide that has been used extensively for the control of aedes albopictus and aedes aegypti, the major vectors for viral diseases, such as dengue fever, zika and chikungunya. resistance to temephos has been recently detected and associated with the upregulation of carboxylesterases (cces) through gene amplification, in both species. here, we expressed the cceae3a genes which showed the most striking up-regulation in resistant aedes strains, using the b ...201627180726
dsrna-mediated targeting of ribosomal transcripts rps6 and rpl26 induces long-lasting and significant reductions in fecundity of the vector aedes aegypti.ribosomal transcripts produce critical proteins that are involved in most cellular production processes. targeting ribosomal transcripts has produced mortality in mites and ticks but the effect of ribosomal transcript knockdown has not been thoroughly examined in mosquitoes. we examine the effects of triggers targeting four ribosomal proteins (rp) transcripts. although no significant mortality was observed after dsrna microinjection and subsequent blood feeding, significant contrasts were observ ...201627180677
toxicological evaluation of essential oil from the leaves of croton tetradenius (euphorbiaceae) on aedes aegypti and mus musculus.for control of aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of croton tetradenius on ae. aegypti larvae and adults. we also evaluated the acute toxicity in mus musculus. the essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. female mice were used for assessing tox ...201627169864
yellow fever cases in asia: primed for an epidemic.there is currently an emerging outbreak of yellow fever in angola. cases in infected travellers have been reported in a number of other african countries, as well as in china, representing the first ever documented cases of yellow fever in asia. there is a large chinese workforce in angola, many of whom may be unvaccinated, increasing the risk of ongoing importation of yellow fever into asia via busy commercial airline routes. large parts of the region are hyperendemic for the related flavivirus ...201627156836
δ-cadinene, calarene and .δ-4-carene from kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes.mosquitoes (diptera: culicidae) are major vectors of important pathogens and parasites. malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis cause millions of deaths every year. mosquito control is being challenging due to the development of pesticide resistance and negative environmental concerns. in this scenario, plants employed in traditional asian medicine may be alternative sources of newer and effective mosquitocides. in this research, we evaluated th ...201627151483
impact of inter- and intra-specific competition among larvae on larval, adult, and life-table traits of aedes aegypti and aedes albopictus females.few studies have taken a comprehensive approach of measuring the impact of inter- and intra-specific larval competition on adult mosquito traits. in this study, the impact of competition aedes aegypti and a. albopictus was quantified over the entire life of a cohort.competitive treatments affected hatch-to-adult survivorship and development time to adulthood of females for both species, but affected median wing length of females only for a. albopictus. competitive treatments had no significant e ...201627141149
multiple qtl determine dorsal abdominal scale patterns in the mosquito aedes aegypti.the mosquito, aedes aegypti (l.) originated in sub-saharan africa as a dark form sylvan species (a. aegypti formosus). evolution of a. aegypti aegypti type form as a human commensal facilitated its colonization of most semitropical and tropical areas. we investigated the genetic basis for abdominal white scale presence that represents the diagnostic for sylvan a. aegypti formosus (scales absent), from type form (scales present) and a. aegypti queenslandensis form (dense scaling). we performed qu ...201627130203
insights into the evolution of chemoreceptor genes superfamily in tyrophagus putrescentiae (acari: acaridae).all living organisms, including animals, plants, fungi, and bacteria, use the olfactory system to recognize chemicals or pheromone from their environment. insects detect a volatile substance using odorant receptors (ors) or gustatory receptors (grs) and ionotropic receptors (irs). the gene families of the olfactory system in acari are still not clear. in this study, we identified seven ors, one gr, and five irs from the transcriptome of the storage mite, tyrophagus putrescentiae schrank. no olfa ...201627113112
detection and distribution of v1016ikdr mutation in the voltage-gated sodium channel gene in aedes aegypti (diptera: culicidae) populations from sergipe state, northeast brazil.aedes aegypti (l.) resistance to pyrethroids was recorded in brazil few years after its introduction as the adulticide in the national dengue control program campaigns. altered susceptibility to pyrethroids had been reported in the state of sergipe, northeast brazil, through biological assays, even before its use against ae. aegypti in the state. metabolic and target-site resistance mechanisms were also revealed in samples from aracaju, the capital of sergipe. herein, we investigated the presenc ...201627113110
recent changes in the local distribution of aedes aegypti (diptera: culicidae) in south florida, usa.disease transmission is directly tied to the spatial distribution of disease vectors. the distribution of aedes aegypti (l.) in the united states has diminished since the introduction of aedes albopictus (skuse) in the 1980s. however, ae. aegypti persists in some urban areas, particularly in south florida. the pattern of habitat segregation of these two species is well documented, but the consistency of this phenomenon over time is unknown. to examine the dynamics of the local distributions of t ...201627113103
characteristics and efficacy of three commercial handheld thermal foggers with pyrethroid insecticides against three species of mosquitoes.the field study's objectives were to compare the physical characteristics as well as efficacy with multiple insecticides for bonide(®) fog rx insect fogger, black flag(®) propane insect fogger, and burgess(®) outdoor propane insect fogger. evaluations were conducted with 7 machine chemical combinations, 3 depths of spray, and 3 species of laboratory-reared mosquitoes, anopheles quadrimaculatus, culex quinquefasciatus, and aedes aegypti. combinations of these factors were analyzed in conjunction ...201627105215
age-stage, two-sex life table characteristics of aedes albopictus and aedes aegypti in penang island, malaysia.the life table developmental attributes of laboratory colonies of wild strains of aedes albopictus and aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (r0), and mean generation time (t). the total preadult development time was 9.47 days for ae. albopictus and 8.76 days fo ...201627105211
comparative analysis of midgut bacterial communities in three aedine mosquito species from dengue-endemic and non-endemic areas of rajasthan, india.dengue viruses are transmitted to humans through the bites of infected female aedine mosquitoes. differences in the composition and structure of bacterial communities in the midguts of mosquitoes may affect the vector's ability to transmit the disease. to investigate and analyse the role of midgut bacterial communities in viral transmission, midgut bacteria from three species, namely stegomyia aegypti (= aedes aegypti), fredwardsius vittatus (= aedes vittatus) and stegomyia albopicta (= aedes al ...201627094337
profile distribution of juvenile aedes species in an urban area of malaysia.dengue fever (df) is an urban vector-borne disease transmitted by aedes aegypti and aedes albopictus. both species deposit their eggs in favorable breeding sites either in natural or artificial containers. an understanding of their habitat characteristics is crucial in curbing df outbreaks201627076510
homology modeling and molecular docking studies of drosophila and aedes sex peptide receptors.the drosophila melanogaster sex peptide receptor (drmspr), which is a g protein-coupled receptor (gpcr), is known as the specific receptor for sex peptide (sp). it is responsible for the reproductive behavior in the drosophila model system; in particular, it is involved in the post-mating responses such as the increase in egg-laying ability and decrease in receptivity in females. in a previous study, we discovered a small molecule agonist of drmspr for the first time, which could not, however, a ...201627060892
functional validation of the carbon dioxide receptor in labial palps of helicoverpa armigera moths.adult moths possess an organ in their labial palps, the labial-palp pit organ, which is specialized for sensing carbon dioxide (co2). they use co2 as a cue to detect healthy plants and find food or lay eggs on them. the molecular bases of the co2 receptor in drosophila melanogaster and aedes aegypti have been reported, but the molecular mechanisms of the co2 receptor in lepidoptera remains elusive. in this study, we first re-examined three putative helicoverpa armigera co2 gustatory receptor gen ...201627060445
molecular cloning, sequence analysis and developmental stage expression of a putative septin gene fragment from aedes albopictus (diptera: culicidae).septins belong to gtpases that are involved in vital cellular activities, including cytokinesis. although present in many organisms, they are yet to be isolated from aedes albopictus. this study reports for the first time on a serendipitous isolation of a partial septin sequence from ae. albopictus and its developmental expression profile. the ae. albopictus partial septin sequence contains 591 nucleotides encoding 197 amino acids. it shares homology with several insect septin genes and has a cl ...201627059016
biological and chemical characterization of a new larvicide ovitrap made of plastic with pyriproxyfen incorporated for aedes aegypti (diptera: culicidae) control.aedes aegypti (l.) is a species of international concern because of its ability to transmit serious human arboviral diseases including yellow fever, dengue, and chikungunya, which have spread to all continents. ovitraps are containers constructed to imitate aedes' natural breeding sites and have been used for many decades as a sensitive and inexpensive surveillance tool for detecting the presence of container-inhabiting mosquitoes. in addition to their value for vector surveillance, various ovit ...201627052297
evaluation of clove oil, icaridin, and transfluthrin for spatial repellent effects in three tests systems against the aedes aegypti (diptera: culicidae).one essential oil (clove oil), one skin repellent (icaridin), and one insecticide (transfluthrin) were tested for spatial repellent effects against non-blood-fed female aedes aegypti (l.) mosquitoes. the compounds were tested in acetone dilution series using a y-olfactometer, a double cage system, and a double room system. all compounds exhibited spatial repellent effects at certain concentrations. clove oil required relative high dosages to cause high effects (y-olfactometer 6 mg, double cage 6 ...201627651437
antimosquito property of petroselinum crispum (umbellifereae) against the pyrethroid resistant and susceptible strains of aedes aegypti (diptera: culicidae).the increasing and widespread resistance to conventional synthetic insecticides in vector populations has underscored the urgent need to establish alternatives in the mosquito management system. this study was carried out with the aim to investigate the antimosquito property, larvicidal and adulticidal potential, of plant products against both the pyrethroid-susceptible and resistant strains of aedes aegypti. seventeen plant products, including essential oils and ethanolic extracts, were obtaine ...201627638800
artemisia absinthium-borne compounds as novel larvicides: effectiveness against six mosquito vectors and acute toxicity on non-target aquatic organisms.the eco-friendly control of mosquito vectors is a crucial challenge of public health importance. here we evaluated the larvicidal potential of artemisia absinthium essential oil (eo) and its three major chemical constituents against six mosquito vectors: anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. the eo was obtained by leaf hydro-distillation. its chemical composition was analyzed using gas chromatography-mass s ...201627630101
aedes aegypti in rural areas: public health implications. 201627622476
[zika: a defeat in the struggle against aedes aegypti]. 201627622433
biosynthesis of silver nanoparticles from mangrove plant (avicennia marina) extract and their potential mosquito larvicidal property.to identify the larvicidal activities of silver nanoparticles synthesised with avicennia marina leaf extract against the larvae of aedes aegypti and anopheleus stephensi, in vitro larvicidal activities such as lc50 and lc90 were assessed. further, characterisation such as uv and ftir analysis were carried out for the synthesised silver nanoparticles. the lc50 value of the synthesised silver nanoparticles was identified as 4.374 and 7.406 mg/l for an. stephensi and ae. aegypti larvae respectively ...201627605825
raed a 4: a new 67-kda aedes aegypti mosquito salivary allergen for the diagnosis of mosquito allergy.accurate diagnosis of mosquito allergy has been hampered by the laborious task of obtaining mosquito salivary allergens. we have previously studied 3 recombinant (r) aedes aegypti mosquito salivary allergens: raed a 1, raed a 2 and raed a 3. here, we report the expression, purification, identification and evaluation of raed a 4, a 67-kda α-glucosidase.201627603527
[effectiveness of commercial repellents against aedes aegypti (l.) in yucatan, méxico].we assessed the efficacy of commercial repellents available in yucatan against aedes aegypti, vector of dengue, chikungunya and zika.201627599081
wolbachia restricts insect-specific flavivirus infection in aedes aegypti cells.mosquito-borne viruses are known to cause disease in humans and livestock and are often difficult to control due to the lack of specific antivirals and vaccines. the wolbachia endosymbiont has been widely studied for its ability to restrict positive-strand rna virus infection in mosquitoes, although little is known about the precise antiviral mechanism. in recent years, a variety of insect-specific viruses have been discovered in mosquitoes and an interaction with mosquito-borne viruses has been ...201627692043
detecting multiple dna human profile from a mosquito blood meal.criminal traces commonly found at crime scenes may present mixtures from two or more individuals. the scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime. thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid in suspect investigation. the aim of the study was to obtain a single aedes aegypti mosquito profile from a human dna mixture containing genet ...201627706606
excito-repellency of citrus hystrix dc leaf and peel essential oils against aedes aegypti and anopheles minimus (diptera: culicidae), vectors of human pathogens.the essential oils of kaffir lime (citrus hystrix dc.) at four different concentrations (0.5, 1.0, 2.5, and 5.0% v/v) were studied for their repellency, excitation, and knockdown properties against laboratory strains of aedes aegypti (l.) and anopheles minimus theobald using an excito-repellency test system. both contact and noncontact escape responses to leaf- and peel-derived kaffir lime oils were observed. comparing unadjusted escape responses for an. minimus, leaf oil had strong combined irr ...201627694144
identification and expression analysis of two 14-3-3 proteins in the mosquito aedes aegypti, an important arboviruses vector.the 14-3-3 proteins are evolutionarily conserved acidic proteins that form a family with several isoforms in many cell types of plants and animals. in invertebrates, including dipteran and lepidopteran insects, only two isoforms have been reported. 14-3-3 proteins are scaffold molecules that form homo- or heterodimeric complexes, acting as molecular adaptors mediating phosphorylation-dependent interactions with signaling molecules involved in immunity, cell differentiation, cell cycle, prolifera ...201627592842
dengue in a changing climate.dengue is the world's most important arboviral disease in terms of number of people affected. over the past 50 years, incidence increased 30-fold: there were approximately 390 million infections in 2010. globalization, trade, travel, demographic trends, and warming temperatures are associated with the recent spread of the primary vectors aedes aegypti and aedes albopictus and of dengue. overall, models project that new geographic areas along the fringe of current geographic ranges for aedes will ...201627475051
[translation into practice of empowerment strategies for dengue prevention: facilitators and barriers].objective to identify facilitators and barriers to the process of translating community empowerment strategies into the practice of the national aedes aegypti control program in cuba. methods a case study of the process was conducted from 2005 to 2009 at national and provincial levels of the control program. data from participant observation, in-depth interviews, and document analysis were triangulated and analyzed inductively and deductively. results the main facilitators identified included a ...201627754520
isolation and identification of mosquito (aedes aegypti) biting-deterrent compounds from the native american ethnobotanical remedy plant hierochloë odorata (sweetgrass).hierochloë odorata (l.) p. beauv. (poaceae), commonly known as sweetgrass, has documented use as an insect repellent by the flatheads of montana and blackfoot of alberta. both the flatheads of montana and blackfoot of alberta would use braided plant material in a sachet in clothing or burn them from one end as incense, air/clothing freshener, and insect repellent. this study evaluated the insect-repellent properties of this plant using an in vitro mosquito aedes aegypti feeding bioassay-directed ...201627744691
dengue related osteonecrosis of the maxillary dento-alveolar bone.dengue is a mosquito transmitted flaviviral infection which can give rise to severe haemorrhage (dengue haemorrhagic fever/dhf) and with capillary leakage induces hypovolemic shock (dengue shock syndrome/dss). although dengue symptoms and complications have been known for many decades there has only been one documented case of osteonecrosis of the maxilla which was treated by excision of the necrotic bone. in this case of dengue infection, extensive maxillary osteonecrosis and minimal root resor ...201627743399
pyrethroid resistance in aedes aegypti and aedes albopictus: important mosquito vectors of human diseases.aedes aegypti and a. albopictus mosquitoes are vectors of important human disease viruses, including dengue, yellow fever, chikungunya and zika. pyrethroid insecticides are widely used to control adult aedes mosquitoes, especially during disease outbreaks. herein, we review the status of pyrethroid resistance in a. aegypti and a. albopictus, mechanisms of resistance, fitness costs associated with resistance alleles and provide suggestions for future research. the widespread use of pyrethroids ha ...201627742355
mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats.mosquitoes are insects of interest because several species vector disease-causing pathogens to humans and other vertebrates. we previously reported that mosquitoes from long-term laboratory cultures require living bacteria in their gut to develop, but development does not depend on particular species of bacteria. here, we focused on three distinct but interrelated areas of study to better understand the role of bacteria in mosquito development by studying field and laboratory populations of aede ...201627718295
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