Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| antibacterial compounds from mushrooms ii: lanostane triterpenoids and an ergostane steroid with activity against bacillus cereus isolated from fomitopsis pinicola. | anti- bacillus cereus bioassay-guided fractionation of a crude extract of the american mushroom, fomitopsis pinicola, was performed using thin-layer chromatography, sephadex lh-20 column chromatography, and preparative-scale hplc. five lanostane triterpenoids (1-5) and one ergostane steroid (6) were isolated and identified. compound 1 is a new lanostane triterpenoid, and its structure was determined using 1d and 2d nmr experiments, hr-ms, and physical data. each of the purified compounds (1-6) w ... | 2010 | 19847745 |
| comparative analysis of transcriptional and physiological responses of bacillus cereus to organic and inorganic acid shocks. | comparative phenotype and transcriptome analyses were performed with bacillus cereus atcc 14579 exposed to ph 5.5 set with different acidulants including hydrochloric acid (hcl), lactic acid (hl) and acetic acid (hac). phenotypes observed included a decreased growth rate (with hcl), bacteriostatic and bactericidal conditions, with 2mm undissociated hac or hl, and 15mm undissociated hac, respectively. in the latter condition a concomitant decrease in intracellular atp levels was observed. the tra ... | 2010 | 19853945 |
| structure and regulation of the gab gene cluster, involved in the gamma-aminobutyric acid shunt, are controlled by a sigma54 factor in bacillus thuringiensis. | the structure and regulation of the gab gene cluster, involved in gamma-aminobutyric acid (gaba) shunt, were studied by characterizing gabt and gabd genes cloned from bacillus thuringiensis. deletions of the gabt and gabd genes in b. thuringiensis strain hd-73 did not affect the growth of mutant strains in rich culture media, but the growth of a gabt deletion mutant strain was reduced in basic media (containing 0.2% gaba). genome analysis indicates that the structure of the gab gene cluster in b ... | 2010 | 19854901 |
| sphingomyelinase-induced phase transformations: causing morphology switches and multiple-time-domain ceramide generation in model raft membranes. | sphingomyelinase (smase) has been shown to be involved in a variety of cell regulation processes by reorganizing the cell membrane morphology. here we report that smase can induce a reaction-induced and a solvent-mediated phase transformation, causing switches of three stationary membrane morphologies and multiple-time-domain ceramide generation in model raft membranes. the reaction-induced phase transformation, triggered by the addition of smase, transforms a pre-existing morphology to a long-l ... | 2010 | 19863058 |
| the chemical composition and antimicrobial activity of the leaf oil of cupressus lusitanica from monteverde, costa rica. | the essential oils from the leaves of three different individuals of cupressus lusitanica were obtained by hydrodistillation and analyzed by gas chromatography - mass spectrometry. a total of 49 compounds were identified in the leaf oils. the major components of c. lusitanica leaf oil were +¦-pinene (40%-82%), limonene (4%-18%), isobornyl acetate (up to 10%) and cis-muurola-4(14),5-diene (up to 7%). the essential oil was screened for antimicrobial activity, and it showed antibacterial activity a ... | 2010 | 21808533 |
| [microbiological evaluation of ready-to-eat foods manufactured by small costa rican industries]. | ready-to-eat (rte) foods are processed foodstuffs which have gained popularity in recent times because they can be ingested without further thermic treatments. in this work, the microbiological quality and safety of 90 samples of rte foods manufactured by small costa rican industries was determined to evaluate whether they represent a public health risk. twenty-six samples of pickled vegetables, 18 dips, 18 salads, and 12 sweet treats were studied. each sample was analyzed with regard to its ph, ... | 2010 | 21427886 |
| antimicrobial activity of plant essential oils against bacterial and fungal species involved in food poisoning and/or food decay. | the currative properties of aromatic and medicinal plants have been recognized since ancient times and, more recently, the antimicrobial activity of plant essential oils has been used in several applications, including food preservation. the purpose of this study was to create directly comparable, quantitative data on the antimicrobial activity of some plant essential oils prepared in the national institute of research-development for chemistry and petrochemistry, bucharest to be used for the fu ... | 2010 | 21462837 |
| evaluation of antibacterial activity of 3-butenyl, 4-pentenyl, 2-phenylethyl, and benzyl isothiocyanate in brassica vegetables. | this study investigated antibacterial activities of 4 isothiocyanates (3-butenyl, 4-phentenyl, 2-phenylethyl, and benzyl isothiocyanate) against 4 gram-positive bacteria (bacillus cereus, bacillus subtilis, listeria monocytogenes, and staphylococcus aureus) and 7 gram-negative bacteria (aeromonas hydrophila, pseudomonas aeruginosa, salmonella choleaesuis, salmonella enterica, serratia marcescens, shigella sonnei, and vibrio parahaemolyticus) by an agar disc diffusion assay. benzyl isothiocyanate ... | 2010 | 21535549 |
| antioxidative and antibacterial effects of seeds and fruit rind of nutraceutical plants belonging to the fabaceae family. | in the present study, the seeds and fruit rind of six plants of the fabaceae family were selected to evaluate their potential as antioxidant and antibacterial agents. the dried powders were individually extracted with various organic solvents by the cold percolation method, were evaluated for antibacterial activity and methanol extracts used for antioxidant activities. total phenol, protein and sugar contents were also measured. antioxidant activities were measured by dpph free radical scavengin ... | 2010 | 21776481 |
| the role of two families of bacterial enzymes in putrescine synthesis from agmatine via agmatine deiminase. | putrescine, one of the main biogenic amines associated to microbial food spoilage, can be formed by bacteria from arginine via ornithine decarboxylase (odc), or from agmatine via agmatine deiminase (agdi). this study aims to correlate putrescine production from agmatine to the pathway involving n-carbamoylputrescine formation via addi (the agua product) and n-carbamoylputrescine amidohydrolase (the agub product), or putrescine carbamoyltransferase (the ptca product) in bacteria. pcr methods were ... | 2010 | 21404211 |
| antibacterial efficacy of raw and processed honey. | in vitro antibacterial activity of methanol, ethanol, and ethyl acetate extracts of raw and processed honey was tested against gram-positive bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, enterococcus faecalis, and micrococcus luteus) and gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, and salmonella typhi). both types of honey showed antibacterial activity against tested organisms with the zone of inhibition (zoi) ranging from 6.94 to 37.94 mm, while e. c ... | 2010 | 21350671 |
| amino acids improve acid tolerance and internal ph maintenance in bacillus cereus atcc14579 strain. | this study investigated the involvement of glutamate-, arginine- and lysine-dependent systems in the acid tolerance response (atr) of bacillus cereus atcc14579 strain. cells were grown in a chemostat at external ph (ph(e)) 7.0 and 5.5. population reduction after acid shock at ph 4.0 was strongly limited in cells grown at ph 5.5 (acid-adapted) compared with cells grown at ph 7.0 (unadapted), indicating that b. cereus cells grown at low ph(e) were able to induce a marked atr. glutamate, arginine a ... | 2010 | 21356439 |
| detection and quantification of spoilage and pathogenic bacillus cereus, bacillus subtilis and bacillus licheniformis by real-time pcr. | a new primer-probe set for the detection and quantification of bacillus cereus, bacillus licheniformis and bacillus subtilis by real-time pcr (rti-pcr) was developed. for it, forty-eight strains belonging to these species were considered. the dna of these strains was isolated and a fragment of the 16s rrna gene amplified. the amplicons were sequenced and the obtained sequences were aligned with reference sequences from the genbank. for the development of the real-time pcr (rti-pcr) methodology b ... | 2010 | 21356471 |
| [myonecrosis in users of injecting drugs (a clinical case)]. | myonecrosis remains one of the severest manifestations of skin and soft tissue infections. clostridia (c. perfringens, c. novyi, c. septicum, c. sordellii, c. histolyticum) are dominant and staphylococcus aureus, streptococcus pyogenes, bacillus cereus, and bacteriodes fragilis are much less in the etiology of myonecrosis. cases of gas gangrene have recently become more frequent among injection drug users all over the world. russia has become the largest opiate market in europe and consumption o ... | 2010 | 21381347 |
| akara ogbomoso: microbiological examination and identification of hazards and critical control points. | akara ogbomoso was examined toward the establishment of hazard analysis and critical control point (haccp). the akara was produced in residential buildings with the attendant consequence of contamination. there was ample growth of aerobes, coliforms, staphylococci, shigella and yeast/mold from the samples, water and cowpea pastes. microbial contaminations occur through the processing, which can be corrected through education by adopting good hygienic and manufacturing practices. the critical con ... | 2010 | 21339157 |
| nature versus nurture in two highly enantioselective esterases from bacillus cereus and thermoanaerobacter tengcongensis. | there is an increasing need for the use of biocatalysis to obtain enantiopure compounds as chiral building blocks for drug synthesis such as antibiotics. the principal findings of this study are: (i) the complete sequenced genomes of bacillus cereus atcc 14579 and thermoanaerobacter tengcongensis mb4 contain a hitherto undescribed enantioselective and alkaliphilic esterase (bcest and ttest respectively) that is specific for the production of (r)-2-benzyloxy-propionic acid ethyl ester, a key inte ... | 2010 | 21255307 |
| studies with bioengineered nisin peptides highlight the broad-spectrum potency of nisin v. | nisin a is the most thoroughly investigated member of the lantibiotic family of antimicrobial peptides. in addition to a long history of safe use as a food antimicrobial, its activity against multi-drug resistant pathogens has resulted in a renewed interest in applying nisin as a chemotherapeutic to treat bacterial infections. the wealth of nisin-related information that has been generated has also led to the development of the biotechnological capacity to engineer novel nisin variants with a vi ... | 2010 | 21255345 |
| comparative proteome analysis of bacillus anthracis with pxo1 plasmid content. | bacillus anthracis the causative agent of anthrax, is an important pathogen among the bacillus cereus group of species because of its physiological characteristics and its importance as a biological warfare agent. tripartite anthrax toxin proteins and a poly-d-glutamic acid capsule are produced by b. anthracis vegetative cells during mammalian hosts infection and when cultured in conditions that are thought to mimic the host environment. to identify the factors regulating virulence in b. anthrac ... | 2010 | 21221933 |
| bacillus gaemokensis sp. nov., isolated from foreshore tidal flat sediment from the yellow sea. | a gram-positive, rod-shaped, endospore-forming organism, strain bl3-6(t), was isolated from tidal flat sediments of the yellow sea in the region of tae-an. a 16s rrna gene sequence analysis demonstrated that this isolate belongs to the bacillus cereus group, and is closely related to bacillus mycoides (99.0% similarity), bacillus thuringiensis (99.0%), bacillus weihenstephanensis (99.0%), bacillus cereus (98.9%), bacillus anthracis (98.8%), and bacillus pseudomycoides (98.1%). the phylogenetic d ... | 2010 | 21221948 |
| decolorization of water soluble azo dyes by bacterial cultures, isolated from dye house effluent. | the aim of this work is to isolate and characterize bacterial isolates form dye house effluent, and to check their ability of decolorizing sulfonated azo dyes, and also to study influence of various environmental parameters on same process. among seven gram positive bacterial isolates obtained form dye house effluent, m1 (bacillus cereus) and m6 were proved to be more potent for decolorizing sulfonated azo dyes under aerobic conditions. maltose as carbon source and peptone as nitrogen source enh ... | 2010 | 20392633 |
| an integrated, self-contained microfluidic cassette for isolation, amplification, and detection of nucleic acids. | a self-contained, integrated, disposable, sample-to-answer, polycarbonate microfluidic cassette for nucleic acid-based detection of pathogens at the point of care was designed, constructed, and tested. the cassette comprises on-chip sample lysis, nucleic acid isolation, enzymatic amplification (polymerase chain reaction and, when needed, reverse transcription), amplicon labeling, and detection. on-chip pouches and valves facilitate fluid flow control. all the liquids and dry reagents needed for ... | 2010 | 20401537 |
| production, secretion and biological activity of bacillus cereus enterotoxins. | bacillus cereus behaves as an opportunistic pathogen frequently causing gastrointestinal diseases, and it is increasingly recognized to be responsible for severe local or systemic infections. pathogenicity of b. cereus mainly relies on the secretion of a wide array of toxins and enzymes and also on the ability to undergo swarming differentiation in response to surface-sensing. in this report, the pathogenicity exerted by b. cereus toxins is described with particular attention to the regulatory m ... | 2010 | 22069656 |
| antimicrobial screening of ethnobotanically important stem bark of medicinal plants. | the stem barks are the rich sources of tannins and other phenolic compounds. tannins inhibited the growth of various fungi, yeast, bacteria and virus. hence, ten stem barks of ethnomedicinally important plants were screened for antibacterial and antifungal activities against human pathogenic strains. | 2010 | 21808577 |
| synthesis and anti-microbial screening of novel schiff bases of 3-amino-2-methyl quinazolin 4-(3h)-one. | in the present study, novel schiff bases were synthesized by condensation of 3-amino-2-methyl quinazolin-4-(3h)-ones with different aromatic aldehydes. the 3-amino-2-methyl quinazolin-4-(3h)-one was synthesized from anthranilic acid via the 2-methyl benzoxazin-4-one. the chemical structures of the synthesized compounds were confirmed by means of infrared (ir), (1)h-nmr, (13)c-nmr, mass spectral, and elemental analysis. these compounds were screened for anti-bacterial (staphylococcus aureus atcc ... | 2010 | 22247864 |
| heterotrophic microorganisms in deteriorated medieval wall paintings in southern italian churches. | the campania region in southern italy is noted for its large number of churches that harbour invaluable frescoes, dated from the beginnings of the 4th up to the 13th century. the wall paintings represent an integral part of the monuments, and their deterioration constitutes a potentially significant loss for the world's cultural heritage. heterotrophic microorganisms such as bacteria and mould can grow on the surface of paintings that contain a wide range of organic and inorganic constituents, a ... | 2010 | 18534834 |
| single-chain variable fragment antibodies selected by phage display against the sporozoite surface antigen p23 of cryptosporidium parvum. | cryptosporidium parvum, an apicomplexan parasite transmitted via animal fecal wastes, is the causative agent of cryptosporidiosis. clones were selected from 2 synthetic naïve human single-chain variable fragment (scfv) phagemid libraries that bound to the recombinant p23 protein of c. parvum. panning the tomlinson i and j phagemid libraries resulted in 6 distinct clones. two clones had full-length scfv sequences, while the remaining clones were either truncated or missing a section of the heavy ... | 2009 | 18576885 |
| synthesis and anti-microbial screening of some schiff bases of 3-amino-6,8-dibromo-2-phenylquinazolin-4(3h)-ones. | in the present study, a series of novel schiff bases were synthesized by condensation of 3-amino-6,8-dibromo-2-phenylquinazolin-4(3h)-ones with different aromatic aldehydes via cyclized intermediate 6,8-dibromo-2-phenyl benzoxazin-4-one. the chemical structures were confirmed by means of ir, (1)h nmr, (13)c nmr, mass spectral and elemental analysis. these compounds were screened for anti-bacterial (staphylococcus aureus atcc-9144, staphylococcus epidermidis atcc-155, micrococcus luteus atcc-4698 ... | 2009 | 18603337 |
| reduction of pollutants in pulp paper mill effluent treated by pcp-degrading bacterial strains. | two pcp-degrading bacterial strains, bacillus cereus (itrc-s6) and serratia marcescens (itrc-s7) were used for the treatment of pulp and paper mill effluent at conditions; 1.0% glucose and 0.5% peptone at 30 +/- 1 degrees c at 120 rpm for 168 h of incubation. these two bacterial strains effectively reduced colour (45-52%), lignin (30-42%), bod (40-70%), cod (50-60%), total phenol (32-40%) and pcp (85-90%) within 168 h of incubation. however, the highest reduction in colour (62%), lignin (54%), b ... | 2009 | 18622710 |
| antibacterial iridoid glucosides from eremostachys laciniata. | eremostachys laciniata (l) bunge (family: lamiaceae alt. labiatae; subfamily: lamioideae) is one of the 15 endemic iranian herbs of the genus eremostachys. a decoction of the roots and flowers of e. laciniata has traditionally been taken orally for the treatment of allergies, headache and liver diseases. three antibacterial iridoid glucosides, phloyoside i (1), phlomiol (2) and pulchelloside i (3) have been isolated from the rhizomes of this plant. the structures of these compounds were elucidat ... | 2009 | 18693303 |
| mitracarpus frigidus aerial parts exhibited potent antimicrobial, antileishmanial, and antioxidant effects. | the crude extract and the hexane, ch(2)cl(2), etoac, n-buoh, and hydromethanolic fractions of the aerial parts of mitracarpus frigidus were evaluated against promastigote forms of two species of leishmania (l. chagasi and l. amazonensis), 11 strains of bacteria (staphylococcus aureus, pseudomonas aeruginosa, salmonella enterica sorovar tythimurium, shigella sonnei, klebsiella pneumoniae, escherichia coli, micrococcus luteus, enterococcus faecalis, enterobacter cloacae, streptococcus pyogenes and ... | 2009 | 18644325 |
| purification and some properties of an extracellular ribonuclease with antiviral activity against tobacco mosaic virus from bacillus cereus. | a new ribonuclease (rnase) with tobacco mosaic virus inhibition was isolated and purified from bacillus cereus zh14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of deae-sephadex a-50 column, and gel chromatography of sephacryl s-200hr column. the enzyme was purified approximately 134-fold with a recovery of 9.2%. the rnase had an mw of 75.6 kda in sds-page, which differed from rnases reported previously. the inhibitory activity of the rnase in the purifica ... | 2009 | 18777010 |
| purification and characterization of a solvent and detergent-stable novel protease from bacillus cereus. | a protease-producing bacterium was isolated from slaughterhouse waste samples, hyderabad, india. it was related to bacillus cereus on the basis of 16s rrna gene sequencing and biochemical properties. the protease was purified to homogeneity using ammonium sulfate precipitation, and ion exchange chromatography with a fold purification of 1.8 and a recovery of 49%. the enzyme had a relative molecular weight of 28kda, ph and temperature optima for this protease were 10 and 60 degrees c. the activit ... | 2009 | 17616381 |
| [effect of phytosubstances with antioxidant activity on persistence properties of microorganisms]. | the in vitro effect of phytosubstances containing flavonoids and phenylpropanoids on the antilysozyme activity of varioous groups of microorganisms, such as staphylococci, bacilli, enterobacteria and nonfermenting bacteria was studied. the second fraction of the pink carbonic acid extract showed high antioxidant activity and most efficiently inhibited the ability of various microorganisms to inactivate the host lysozyme. the selected phytosubstance could be useful in clinical practice as an addi ... | 2009 | 20415257 |
| physiological traits of the symbiotic bacterium teredinibacter turnerae isolated from the mangrove shipworm neoteredo reynei. | nutrition in the teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria of the teredinibacter clade. the mangrove teredinidae neoteredo reynei is popularly used in the treatment of infectious diseases in the north of brazil. in the present work, the symbionts of n. reynei, which are strictly confined to the host's gills, were conclusively identified as teredinibacter turnerae. symbiont variants obtained in vitro were able to grow using casein a ... | 2009 | 21637522 |
| response of bacillus cereus atcc 14579 to challenges with sublethal concentrations of enterocin as-48. | enterocin as-48 is produced by enterococcus faecalis s48 to compete with other bacteria in their environment. due to its activity against various gram positive and some gram negative bacteria it has clear potential for use as a food preservative. here, we studied the effect of enterocin as-48 challenges on vegetative cells of bacillus cereus atcc 14579 by use of transcriptome analysis. | 2009 | 19863785 |
| inactivation of food pathogen bacillus cereus by photosensitization in vitro and on the surface of packaging material. | the study was focused on the possibility to inactivate food pathogen bacillus cereus by 5-aminolevulinic acid (ala) - based photosensitization in vitro and after adhesion on the surface of packaging material. | 2009 | 19849812 |
| rapid identification of bacterial isolates from aqueous kava (piper methysticum) extracts by polymerase chain reaction and dna sequencing. | fresh kava beverages have a limited shelf life under refrigerated conditions. the objective of this study was to isolate and identify bacteria in aqueous extracts of kava rhizome. | 2009 | 19843208 |
| confinement regulates complex biochemical networks: initiation of blood clotting by "diffusion acting". | this study shows that environmental confinement strongly affects the activation of nonlinear reaction networks, such as blood coagulation (clotting), by small quantities of activators. blood coagulation is sensitive to the local concentration of soluble activators, initiating only when the activators surpass a threshold concentration, and therefore is regulated by mass transport phenomena such as flow and diffusion. here, diffusion was limited by decreasing the size of microfluidic chambers, and ... | 2009 | 19843446 |
| microbiological quality of saffron from the main producer countries. | a microbiological study of saffron spice was undertaken in the context of a european research project (methodologies for implementing international standards for saffron purity and quality, the acronym for which is saffic), analyzing 79 samples obtained from the main producer countries, namely greece, iran, italy, morocco, and spain. current microbiological quality criteria are the same as for other spices, but saffron is added in minute quantities during the cooking process, so the health risk ... | 2009 | 19833050 |
| new cyclopentenones from strain trichoderma sp. ylf-3. | two new cyclopentenones, trichodermone a (1) and trichodermone b (2), together with a known compound, 3-(3-oxocyclopent-1-enyl) propanoic acid (3), were isolated from the strain trichoderma sp. ylf-3. their structures were elucidated on the basis of their spectroscopic data. all compounds were assayed for antibacterial activity. compound 3 showed activity against staphyloccocus aureus and bacillus cereus. | 2009 | 19809916 |
| chemical composition and antimicrobial activity of the extracts of kefe cumin (laser trilobum l.) fruits from different regions. | structural properties (using gas chromatographic analyses: gas chromatography and gas chromatography-mass spectrometry) of some compounds (pure chemicals, essential oils, extracts, etc.) and systematic investigation of antimicrobial activities (with agar dilution and agar diffusion disc methods) were determined in the fruits of kefe cumin (laser trilobum l.) obtained from different regions (germany, india, and turkey). the main components were established as limonene (41.03-72.24%) and perillald ... | 2009 | 19817640 |
| the corynebacterium diphtheriae shaft pilin spaa is built of tandem ig-like modules with stabilizing isopeptide and disulfide bonds. | cell-surface pili are important virulence factors that enable bacterial pathogens to adhere to specific host tissues and modulate host immune response. relatively little is known about the structure of gram-positive bacterial pili, which are built by the sortase-catalyzed covalent crosslinking of individual pilin proteins. here we report the 1.6-a resolution crystal structure of the shaft pilin component spaa from corynebacterium diphtheriae, revealing both common and unique features. the spaa p ... | 2009 | 19805181 |
| [structure and function of sphingomyelinase]. | bacillus cereus is one that causes of opportunistic human infections. sphingomyelinase produced by b. cereus is assumed a virulence factor for the infection. sphingomyelinase from bacillus cereus (bc-smase) is mg(2+)-containing metalloenzyme. bc-smase is a family of neutral smase (nsmase) and mimics the actions of the endogenous mammalian nsmase in causing differentiation, development, and apoptosis. bc-smase may be a good model for the poorly characterized mammalian nsmase. activation of bc-sma ... | 2009 | 19797879 |
| valinomycin biosynthetic gene cluster in streptomyces: conservation, ecology and evolution. | many streptomyces strains are known to produce valinomycin (vlm) antibiotic and the vlm biosynthetic gene cluster (vlm) has been characterized in two independent isolates. here we report the phylogenetic relationships of these strains using both parsimony and likelihood methods, and discuss whether the vlm gene cluster shows evidence of horizontal transmission common in natural product biosynthetic genes. eight streptomyces strains from around the world were obtained and sequenced for three regi ... | 2009 | 19787052 |
| synthesis of ethyl acetate employing celite-immobilized lipase of bacillus cereus mtcc 8372. | a wide range of fatty acid esters can be synthesized by esterification and transesterification reactions catalyzed by lipases in non-aqueous systems. in the present study, immobilization of a purified alkaline extra-cellular lipase of bacillus cereus mtcc 8372 by adsorption on diatomaceous earth (celite) for synthesis of ethyl acetate via transesterification route was investigated. b. cereus lipase was deposited on celite (77% protein binding efficiency) by direct binding from aqueous solution. ... | 2009 | 19789138 |
| the impact of oxygen availability on stress survival and radical formation of bacillus cereus. | both the growth and stress survival of two model bacillus cereus strains, atcc 14579 and atcc 10987, were tested in three different conditions varying in oxygen availability, i.e., aerobic, microaerobic and anaerobic conditions. both b. cereus strains displayed highest growth rates and yields under aerobic conditions, whereas the microaerobic and anaerobic cultures showed similar reduced growth performances. the cells grown and exposed microaerobically and anaerobically were more resistant to he ... | 2009 | 19762101 |
| effect of bile salts on the dna and membrane integrity of enteric bacteria. | enteric bacteria are able to resist the high concentrations of bile encountered throughout the gastrointestinal tract. here we review the current mechanisms identified in the enteric bacteria salmonella, escherichia coli, bacillus cereus and listeria monocytogenes to resist the dangerous effects of bile. we describe the role of membrane transport systems, and their connection with dna repair pathways, in conferring bile resistance to these enterics. we discuss the findings from recent investigat ... | 2009 | 19762477 |
| the dlt operon of bacillus cereus is required for resistance to cationic antimicrobial peptides and for virulence in insects. | the dlt operon encodes proteins that alanylate teichoic acids, the major components of cell walls of gram-positive bacteria. this generates a net positive charge on bacterial cell walls, repulsing positively charged molecules and conferring resistance to animal and human cationic antimicrobial peptides (amps) in gram-positive pathogenic bacteria. amps damage the bacterial membrane and are the most effective components of the humoral immune response against bacteria. we investigated the role of t ... | 2009 | 19767427 |
| identification and classification of bcl genes and proteins of bacillus cereus group organisms and their application in bacillus anthracis detection and fingerprinting. | the bacillus cereus group includes three closely related species, b. anthracis, b. cereus, and b. thuringiensis, which form a highly homogeneous subdivision of the genus bacillus. one of these species, b. anthracis, has been identified as one of the most probable bacterial biowarfare agents. here, we evaluate the sequence and length polymorphisms of the bacillus collagen-like protein bcl genes as a basis for b. anthracis detection and fingerprinting. five genes, designated bcla to bcle, are pres ... | 2009 | 19767469 |
| identification of bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks. | this report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, bacillus anthracis. bacillus cultures were prepared under standardized conditions and inactivated according to a recently developed ms-compatible inactivation protocol for highly pathogenic microorganisms. maldi-tof ms was then emplo ... | 2009 | 19767470 |
| identification of a polymorphic collagen-like protein in the crustacean bacteria pasteuria ramosa. | pasteuria ramosa is a spore-forming bacterium that infects daphnia species. previous results demonstrated a high specificity of host clone/parasite genotype interactions. surface proteins of bacteria often play an important role in attachment to host cells prior to infection. we analyzed surface proteins of p. ramosa spores by two-dimensional gel electrophoresis. for the first time, we prove that two isolates selected for their differences in infectivity reveal few but clear-cut differences in p ... | 2009 | 19770039 |
| characterization of single heat-activated bacillus spores using laser tweezers raman spectroscopy. | heat activation of dormant bacterial spores is a short treatment at a sublethal temperature that potentiates and synchronizes spore germination. in this paper, laser tweezers raman spectroscopy (ltrs) was used to study the heat activation of single spores of bacillus cereus and bacillus subtilis. we measured the raman spectra of single spores without treatment, during heat activation at 65 degrees c (b. cereus) or 70 degrees c (b. subtilis), and following heat activation and cooling to 25 degree ... | 2009 | 19770863 |
| inhibition of salmonella enteritidis by cerein 8a, edta and sodium lactate. | the ability of the bacteriocin cerein 8a to inhibit salmonella enteritidis in combination with edta and sodium lactate was investigated. salmonella enteritidis was incubated with combinations of cerein 8a (3200au/ml) and edta (20, 50, 100 mmol/l) or sodium lactate (200 mmol/l). all treatments caused a significant reduction in the od(600) values of salmonella enteritidis cultures. the addition of cerein 8a plus edta resulted in higher inhibition in comparison with the bacteriocin alone; the great ... | 2009 | 19775768 |
| study on the antibiotic activity of microcapsule curcumin against foodborne pathogens. | curcumin as an edible natural pigment has several biological activities but its use as a food colorant and preservative is restricted due to low stability and insolubility in water. stabilizing it with microencapsulation to obtain microcapsule curcumin can improve its stability and solubility. the microcapsule curcumin was studied for their antibacterial and antifungal activities against some foodborne pathogens and spoilage microbe such as escherichia coli, yersinia enterocolitica, staphylococc ... | 2009 | 19775769 |
| expression of recombinant antibacterial lactoferricin-related peptides from pichia pastoris expression system. | four recombinant antimicrobial peptide (ramp) cdnas, constructed from two goat lactoferricin-related peptide cdnas (glfcin and glfcin ii) with/without (his)(6)-tag, were cloned into ppiczalphac and transformed into pichia pastoris smd1168h. after methanol induction, these ramps were expressed and secreted into broth. they were purified after cm-sepharose (without his-tg), histrap (with his-tg) and sephadex g-25 chromatographies. the yield of purified ramp was 0.15 mg/ml of broth. these 4 ramps w ... | 2009 | 19780544 |
| sympatric soil communities of bacillus cereus sensu lato: population structure and potential plasmid dynamics of pxo1- and pxo2-like elements. | eighty soil-borne bacillus cereus group isolates were collected from two neighbouring geographical sites in belgium. their genetic relationships and population structure were assessed using multilocus sequence typing analysis of five chromosomal genes, while the contribution of extrachromosomal elements to the population dynamics was gauged by the presence, diversity and transfer capacity of pxo1- and pxo2-like plasmids. globally, the bacterial population displayed a broad diversity, including a ... | 2009 | 19780824 |
| effects of moderately high pressure plus heat on the germination and inactivation of bacillus cereus spores lacking proteins involved in germination. | to determine the germination and inactivation of bacillus cereus spores lacking various germination proteins using moderately high pressure (mhp) and heat. | 2009 | 19780952 |
| volatile constituents and antibacterial screening of the essential oil of chenopodium ambrosioides l. growing in nigeria. | the essential oil of the aerial parts of chenopodium ambrosioides l. has been isolated by hydrodistillation and analyzed using gc-ms. the major components were found to be alpha-terpinene (63.1%), p-cymene (26.4%) and ascaridole (3.9%). the oil displayed no antibacterial activity against either gram-positive bacteria bacillus cereus or staphylococcus aureus, or the gram-negative bacterium escherichia coli (mic=1250 microg/ml). a cluster analysis of c. ambrosioides essential oils reveals at least ... | 2009 | 19731609 |
| biosynthesis and transcriptional analysis of thurincin h, a tandem repeated bacteriocin genetic locus, produced by bacillus thuringiensis sf361. | thurincin h, a bacteriocin produced by bacillus thuringiensis sf361 isolated from honey, strongly inhibited the growth of bacillus cereus f4552. the bacteriocin was purified by 65% ammonium sulfate precipitation of the culture supernatant, followed by octyl-sepharose cl-4b and reverse-phase hplc. the molecular mass of the bacteriocin was determined to be 3139.51 da and the 14 amino acids of the bacteriocin at the n-terminus were identified. the complete amino acid sequence of mature thurincin h ... | 2009 | 19732155 |
| in vitro antimicrobial effect of satureja wiedemanniana against bacillus species isolated from raw meat samples. | in this study a total of 30 raw meat samples obtained from ankara, turkey were screened for the presence of bacillus species. among the meat samples analyzed, the predominant species isolated was bacillus circulans; other bacillus species were identified as bacillus firmus, bacillus lentus, bacillus megaterium, bacillus licheniformis, bacillus mycoides, bacillus sphaericus, and bacillus cereus. minced meat samples were more contaminated with bacillus species than sliced beef sample. from these s ... | 2009 | 19735196 |
| microbial toxin's effect on mitochondrial survival by increasing k+ uptake. | we studied the effects of toxins, which inhibited the motility of boar spermatozoa, on rat liver mitochondria. the toxins studied were originally from bacteria isolated from moisture-damaged buildings where inhabitants exhibited symptoms, or from food causing poisoning. some strains of bacillus cereus and streptomyces griseus produced potassium ionophoric peptides cereulide and valinomycin (mikkola, et al., european journal of biochemistry 1999; 263: 112-117). of interest is that channels were f ... | 2009 | 19736254 |
| antibiotic resistance in bacteria: novel metalloenzyme inhibitors. | beta-lactam antibiotics are among the most important drugs used to fight bacterial infection. overuse and misuse of beta-lactam antibiotics has caused the evolution of resistance mechanisms, allowing pathogenic bacteria to survive antibiotic treatment. the major source of resistance to beta-lactam antibiotics occurs through production of enzymes called beta-lactamases capable of catalyzing hydrolysis of the beta-lactam rings in these drug compounds. the metallo-beta-lactamases have become a majo ... | 2009 | 19751419 |
| enteropathogenicity of bacillus cereus isolated from some nigerian foods. | bacillus cereus is a soil bacterium and has been isolated from cooked and uncooked rice, milk and dairy products, meat, spices, garlic, vegetables, cereals and infant formulae. | 2009 | 19761179 |
| production of bioactive metabolites by nocardia levis mk-vl_113. | to isolate and identify the bioactive compounds produced by nocardia levis mk-vl_113. | 2009 | 19708882 |
| assay of enterocin as-48 for inhibition of foodborne pathogens in desserts. | enterocin as-48 was tested against staphylococcus aureus, bacillus cereus, and listeria monocytogenes in different kinds of desserts. the highest activity against s. aureus was detected in baker cream. however, in yogurt-type soy-based desserts and in gelatin pudding, as-48 (175 arbitrary units [au]/g) reduced viable cell counts of s. aureus by only 1.5 to 1.8 log units at most. the efficacy of as-48 in puddings greatly depended on inoculum size, and viable s. aureus counts decreased below detec ... | 2009 | 19722396 |
| the genome and variation of bacillus anthracis. | the bacillus anthracis genome reflects its close genetic ties to bacillus cereus and bacillus thuringiensis but has been shaped by its own unique biology and evolutionary forces. the genome is comprised of a chromosome and two large virulence plasmids, pxo1 and pxo2. the chromosome is mostly co-linear among b. anthracis strains and even with the closest near neighbor strains. an exception to this pattern has been observed in a large inversion in an attenuated strain suggesting that chromosome co ... | 2009 | 19729033 |
| effect of mixed antimicrobial agents and flavors in active packaging films. | active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ... | 2009 | 19711918 |
| an extracytoplasmic function sigma factor controls beta-lactamase gene expression in bacillus anthracis and other bacillus cereus group species. | the susceptibility of most bacillus anthracis strains to beta-lactam antibiotics is intriguing considering that the closely related species bacillus cereus and bacillus thuringiensis typically produce beta-lactamases and the b. anthracis genome harbors two beta-lactamase genes, bla1 and bla2. we show that beta-lactamase activity associated with b. anthracis is affected by two genes, sigp (ba2502) and rsip (ba2503), predicted to encode an extracytoplasmic function sigma factor and an anti-sigma f ... | 2009 | 19717606 |
| inhibition of nitrate reduction by chromium (vi) in anaerobic soil microcosms. | chromium is often found as a cocontaminant at sites polluted with organic compounds. for nitrate-respiring microbes, cr(vi) may be not only directly toxic but may also specifically interfere with n reduction. in soil microcosms amended with organic electron donors, cr(vi), and nitrate, bacteria oxidized added carbon, but relatively low doses of cr(vi) caused a lag and then lower rates of co(2) accumulation. cr(vi) strongly inhibited nitrate reduction; it occurred only after soluble cr(vi) could ... | 2009 | 19684175 |
| expression of bacillus cereus hemolysin ii in bacillus subtilis renders the bacteria pathogenic for the crustacean daphnia magna. | hemolysin ii (hlyii) is a pore-forming toxin of the opportunistic pathogen bacillus cereus. despite our understanding of the mechanism of hlyii cytotoxicity in vitro, many of its characteristics, including potential target cells, conditions of its action and expression, are not known. here we report that the expression of hlyii in bacillus subtilis renders the bacteria hemolytic and is able to kill the crustacean daphnia magna. the hemolytic activity of hlyii-encoded b. subtilis strains in cultu ... | 2009 | 19686345 |
| characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (lepidoptera: noctuidae). | the characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (anticarsia gemmatalis) were the objectives of this study. twelve aerobic and anaerobic isolates of proteolytic bacteria were obtained from the caterpillar gut in calcium caseinate agar. the number of colony forming units (cfus) of proteolytic bacteria was higher when the bacteria were extracted from caterpillars reared on artificial diet rather than on soybean leaves (1.73 +/- 0.35 x 10(3) ... | 2009 | 19689886 |
| changes in membrane fatty acids and murein composition of bacillus cereus and salmonella typhi induced by gamma irradiation treatment. | this present study was carried out to evaluate the effects of gamma irradiation on the fatty acids (fa) and mureins composition of two different radiotolerant bacteria. bacillus cereus lspq 2872 and salmonella typhi atcc 19430 were used for this study. the bacterial strains were treated with a sublethal radiation dose of 1kgy to cause a cellular damage. gas chromatography (gc) and high performance liquid chromatography (hplc) analysis were performed to demonstrate respectively the modification o ... | 2009 | 19651456 |
| bacillus anthracis physiology and genetics. | bacillus anthracis is a member of the bacillus cereus group species (also known as the "group 1 bacilli"), a collection of gram-positive spore-forming soil bacteria that are non-fastidious facultative anaerobes with very similar growth characteristics and natural genetic exchange systems. despite their close physiology and genetics, the b. cereus group species exhibit certain species-specific phenotypes, some of which are related to pathogenicity. b. anthracis is the etiologic agent of anthrax. ... | 2009 | 19654018 |
| complete sequence of three plasmids from bacillus thuringiensis inta-fr7-4 environmental isolate and comparison with related plasmids from the bacillus cereus group. | bacillus thuringiensis is an insect pathogen used worldwide as a bioinsecticide. it belongs to the bacillus cereus sensu lato group as well as bacillus anthracis and b. cereus. plasmids from this group of organisms have been implicated in pathogenicity as they carry the genes responsible for different types of diseases that affect mammals and insects. some plasmids, like paw63 and pbt9727, encode a functional conjugation machinery allowing them to be transferred to a recipient cell. they also sh ... | 2009 | 19654019 |
| production and structural characterization of crystalline silver nanoparticles from bacillus cereus isolate. | to date, biosynthesis of metal nanoparticles has been intensively studied using bacteria and fungi. we have isolated and identified metal resistant bacterial strains from electroplating industries, they produce silver nanoparticles. the reduction reaction of aqueous silver nitrate with bacterial biomass was carried out for 120 h. bacteria produced metallic nanoparticles showed a strong absorbance at surface plasmon resonance wavelength around 420 nm. the size and morphology of these nanoparticle ... | 2009 | 19660920 |
| the synthesis of 2- and 3-aryl indoles and 1,3,4,5-tetrahydropyrano[4,3-b]indoles and their antibacterial and antifungal activity. | a series of 2- and 3-aryl substituted indoles and two 1,3,4,5-tetrahydropyrano[4,3-b]indoles were synthesized from indole and 5-methoxyindole. the 2-aryl indoles were synthesized from the 1-(phenylsulfonyl)indole derivatives using magnesiation followed by iodination. the 2-iodinated compounds were then subjected to suzuki-miyaura reactions. in addition, the 3-aryl indoles were made from the corresponding 3-bromoindoles using suzuki-miyaura reactions. the 1,3,4,5-tetrahydropyrano[4,3-b]indoles we ... | 2009 | 19660944 |
| characterization of lactobacillus spp. isolated from the feces of breast-feeding piglets. | lactobacillus spp., referred to as ij-1 and ij-2, were isolated from the feces of breast-feeding piglets and analyzed for probiotic properties. according to the analyses of 16s rdna sequence, lactobacillus sp. ij-1 showed greater than 99% homology with lactobacillus reuteri dsm 20016(t), and lactobacillus sp. ij-2 had greater than 99% homology with the l. gasseri atcc 33323(t) and l. johnsonii atcc 33200(t). the ph changes in the culture media of lactobacillus sp. ij-1 and lactobacillus sp. ij-2 ... | 2009 | 19664551 |
| positively cooperative binding of zinc ions to bacillus cereus 569/h/9 beta-lactamase ii suggests that the binuclear enzyme is the only relevant form for catalysis. | metallo-beta-lactamases catalyze the hydrolysis of most beta-lactam antibiotics and hence represent a major clinical concern. while enzymes belonging to subclass b1 have been shown to display maximum activity as dizinc species, the actual metal-to-protein stoichiometry and the affinity for zinc are not clear. we have further investigated the process of metal binding to the beta-lactamase ii from bacillus cereus 569/h/9 (known as bcii). zinc binding was monitored using complementary biophysical t ... | 2009 | 19665032 |
| development of a real-time pcr assay for detection and quantification of enterotoxigenic members of bacillus cereus group in food samples. | a highly sensitive real-time pcr (qpcr) procedure, targeting the phosphatidylcholine-specific phospholipase c gene (pc-plc), was developed for specific detection and quantification of strains belonging to bacillus cereus group. the target region was selected based on the enterotoxigenic profiles of 75 bacillus strains. the inclusivity and exclusivity of the rti-pcr assay were assessed with 59 isolates of the b. cereus group, 16 other bacillus spp., and 4 non-bacillus strains. the assay was also ... | 2009 | 19665814 |
| characterization of sucrose-glutamic acid maillard products (sgmps) degrading bacteria and their metabolites. | two aerobic bacteria rnbs1 and rnbs3 capable to degrade and utilize sucrose-glutamic acid maillard products (sgmps) as carbon, nitrogen and energy source were isolated and characterized as alcaligenes faecalis (dq659619) and bacillus cereus (dq659620) respectively by 16s rrna gene sequence analysis. in present study, mixed bacterial culture was found more effective compared to axenic culture rnbs1 and rnbs3 decolourizing 73.79%, 66.80% and 62.56% sgmps, respectively. the sgmps catabolizing enzym ... | 2009 | 19665891 |
| the cca anticodon specifies separate functions inside and outside translation in bacillus cereus. | bacillus cereus 14579 encodes two trnas with the cca anticodon, trna(trp) and trna(other). trna(trp) was separately aminoacylated by two enzymes, trprs1 and trprs2, which share only 34% similarity and display different catalytic capacities and specificities. trprs1 was 18-fold more proficient at aminoacylating trna(trp) with trp, while trprs2 more efficiently utilizes the trp analog 5-hydroxy trp. trna(other) was not aminoacylated by either trprs but instead by the combined activity of lysrs1 an ... | 2009 | 19667754 |
| antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens. | crude extracts were prepared using the solvent ethanol from the seaweeds viz., gracilaria edulis, calorpha peltada and hydroclothres sp. and screening for their antibacterial activity against six bacterial pathogens were also carried out. the test bacterial strains were escherichia coli, enterobacter aerogenes, staphylococcus aureus, pseudomonas aeruginosa, streptococcus faecalis and bacillus cereus. ethanol extract of gracilaria edulis inhibited growth of all the test organisms except bacillus ... | 2009 | 19673167 |
| novel and unique diagnostic biomarkers for bacillus anthracis infection. | a search for bacterium-specific biomarkers in peripheral blood following infection with bacillus anthracis was carried out with rabbits, using a battery of specific antibodies generated by dna vaccination against 10 preselected highly immunogenic bacterial antigens which were identified previously by a genomic/proteomic/serologic screen of the b. anthracis secretome. detection of infection biomarkers in the circulation of infected rabbits could be achieved only after removal of highly abundant s ... | 2009 | 19648366 |
| biofilm formation and cell surface properties among pathogenic and nonpathogenic strains of the bacillus cereus group. | biofilm formation by 102 bacillus cereus and b. thuringiensis strains was determined. strains isolated from soil or involved in digestive tract infections were efficient biofilm formers, whereas strains isolated from other diseases were poor biofilm formers. cell surface hydrophobicity, the presence of an s layer, and adhesion to epithelial cells were also examined. | 2009 | 19648377 |
| [a case of sepsis in ilbw infant caused by bacillus cereus]. | bacillus cereus is an aerobic or facultative anaerobic gram-positive rod which is ubiquitous in the environment. the incidence of neonatal infections is very low. the clinical course is serious, usually life- threatening or permanently damaging the central nervous system. the immature immune system, subjected to invasive procedures, increases the morbidity risk in this age group, especially in extremely low birth weight infants. diagnostic difficulties and insusceptibility to ''first- line'' ant ... | 2009 | 19648658 |
| antimicrobial susceptibility, beta-lactamase and enterotoxin production in bacillus cereus isolates from clinical and food samples. | the antimicrobial susceptibility of 30 clinical and 30 food bacillus cereus isolates was determined. all isolates were susceptible to streptomycin, ciprofloxacin and gentamicin, 90 % of them to clindamycin and vancomycin, and 67 % to erythromycin. all isolates were resistant to amoxicillin with clavulanic acid, ampicillin, cefotaxime, ciprofloxacin, cloxacillin, cefotaxime with clavulanic acid and penicillin. the mic values (determined by e-tests) were 48-256 mg/l for ampicillin, 0.19-1.5 mg/l f ... | 2009 | 19649741 |
| superdormant spores of bacillus species have elevated wet-heat resistance and temperature requirements for heat activation. | purified superdormant spores of bacillus cereus, b. megaterium, and b. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations, thus allowing isolation of the superdormant spores, and even with alternate germinants. however, these superdormant spores exhibited significant germination with the original or a ... | 2009 | 19592590 |
| complete genome sequence of the extremophilic bacillus cereus strain q1 with industrial applications. | bacillus cereus strain q1 was isolated from a deep-subsurface oil reservoir in the daqing oil field in northeastern china. this strain is able to produce biosurfactants and to survive in extreme environments. here we report the finished and annotated genome sequence of this organism. | 2009 | 19060151 |
| effect of olive powder on the growth and inhibition of bacillus cereus. | a study was carried out to investigate the antimicrobial effect of olive powder on bacillus cereus. microorganism spores were inoculated in nutrient broth containing different olive powder concentrations and incubated at 32 degrees , 20 degrees , and 7 degrees c. results indicated that this food ingredient had a bacteriostatic effect. it was observed that b. cereus cells did not grow at 7 degrees c. in the cases where growth occurred, it was modelled and parameters were deduced. in general the h ... | 2009 | 19061367 |
| survival of foodborne pathogens in grain products and the effect of catechins. | we investigated the survival of bacterial pathogens in grain products. salmonella enteritidis and staphylococcus aureus survived for more than 3 weeks in grain flakes. although s. enteritidis in grain flakes reached a level of less than 10(2) cfu/g, the surviving organisms grew rapidly in milk and reached 10(9) cfu/g after 25-hr incubation. when catechins were present on the grain flakes, the numbers of s. enteritidis and s. aureus were significantly decreased. in addition, catechins reduced the ... | 2009 | 19602859 |
| broad range evaluation of the matrix solubilization (matrix lysis) strategy for direct enumeration of foodborne pathogens by nucleic acids technologies. | a previously published rapid (<5 h) proof-of-concept protocol for the concentration of the foodborne pathogen listeria monocytogenes from milk, based on the solubilization of the food matrix, was further evaluated. the original protocol was modified to detect gram-negative and other gram-positive bacteria and to broaden the range of food matrices by using lutensol instead of sodium dodecyl sulfate as the main detergent in the buffer. a new protocol using a protease and sucrose buffer was establi ... | 2009 | 19610333 |
| simultaneous identification of seven foodborne pathogens and escherichia coli (pathogenic and nonpathogenic) using capillary electrophoresis-based single-strand conformation polymorphism coupled with multiplex pcr. | the objective of this study was to develop a novel technique for parallel analysis of eight important foodborne microbes using capillary electrophoresis-based single-strand conformation polymorphism (ce-sscp) coupled with multiplex pcr. specific primers for multiplex pcr amplification of the 16s rrna gene were designed, corresponding to eight species of bacteria, including escherichia coli, clostridium perfringens, campylobacter jejuni, salmonella enterica, listeria monocytogenes, vibrio parahae ... | 2009 | 19610337 |
| a response surface model to describe the effect of temperature and ph on the growth of bacillus cereus in cooked rice. | this study was performed to develop a predictive model for the growth rate of bacillus cereus in cooked rice. a response surface methodology (rsm) was used with a combination of storage temperature (10 to 40 degrees c) and ph value (5.4 to 6.8). the growth curves generated under different conditions were fitted using a modified gompertz equation, and the relationship of the growth rate to the growth curves was modeled using an rsm quadratic polynomial equation. the predictive model was significa ... | 2009 | 19610344 |
| bacillus cereus in refrigerated milk submitted to different heat treatments. | the possibility of the survival, germination, and multiplication of bacillus cereus in extended-shelf-life milk prompted research into the occurrence of the bacteria in refrigerated milk submitted to different heat treatments. samples were submitted to ultrapasteurization (138 degrees c for 2 s), "superpasteurization" (96 degrees c for 13 s), and pasteurization (74 degrees c for 15 s) and stored under refrigeration at 4 +/- 2 degrees c for up to 6 weeks. the milk was analyzed for its sensory qua ... | 2009 | 19610345 |
| synthesis, characterization and evaluation of antibacterial activity of some thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties. | a series of thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties were synthesized starting from 5-[4-(4-x-phenylsulfonyl)phenyl]-4h-1,2,4-triazole-3-thioles 3a-c, x=h, cl, br. thus, alkylation of 1,2,4-triazoles 3 with phenacyl bromide or 4-bromophenacyl bromide afforded s-substituted 1,2,4-triazoles 4, 5. these new intermediates 4 and 5, in the presence of h(2)so(4) (c), were cyclized to 2-[4-(4-x-phenylsulfonyl)phenyl]-6-(4-y-phenyl)[1,3]thiazolo[3,2-b]-[1,2,4]-triazoles 6, 7 ... | 2009 | 19616347 |
| the small acid soluble proteins (sasp alpha and sasp beta) of bacillus weihenstephanensis and bacillus mycoides group 2 are the most distinct among the bacillus cereus group. | the bacillus cereus group includes bacillus anthracis, b. cereus, bacillus thuringiensis, bacillus mycoides and bacillus weihenstephanensis. the small acid soluble spore protein (sasp) beta has been previously demonstrated to be among the biomarkers differentiating b. anthracis and b. cereus; sasp beta of b. cereus most commonly exhibits one or two amino acid substitutions when compared to b. anthracis. sasp alpha is conserved in sequence among these two species. neither sasp alpha nor beta for ... | 2009 | 19616612 |
| antimicrobial, mechanical, and barrier properties of cassava starch-chitosan films incorporated with oregano essential oil. | the physicochemical and antimicrobial properties of starch-chitosan films incorporated with oregano essential oil (oeo) have been investigated. the antimicrobial effects of starch-chitosan-oeo films against bacillus cereus, escherichia coli, salmonella enteritidis, and staphylococcus aureus were determined by the disk inhibition zone method. the film mechanical properties, water vapor permeability (wvp), fourier transform infrared spectra (ftir), and thermograms (tga) were also determined. films ... | 2009 | 19627142 |
| differentiation of whole bacterial cells based on high-throughput microarray chip printing and infrared microspectroscopic readout. | using robotic automation, a microarray printing protocol for whole bacterial cells was developed for subsequent label-free and nondestructive infrared microspectroscopic detection. using this contact microspotting system, 24 microorganisms were printed on zinc selenide slides; these were 6 species of listeria, 10 species of vibrio, 2 strains of photobacterium damselae, yersinia enterocolitica 289, bacillus cereus atcc 14529, staphylococcus aureus, atcc 19075 (serotype 104 b), shigella sonnei 201 ... | 2009 | 19630511 |
| literature review on the safety of toyocerin, a non-toxigenic and non-pathogenic bacillus cereus var. toyoi preparation. | bacillus cereus var. toyoi is a naturally occurring, non-toxigenic and non-pathogenic strain of b. cereus. safety studies were conducted on a b. toyoi preparation (toyocerin, including but not limited to enterotoxicity, eye irritation, genotoxicity, acute, subchronic and chronic toxicity studies and human clinical trials. in rabbits, toyocerin did not exhibit enterotoxicity and was only slightly irritating to the eyes. it was non-mutagenic in an ames assay at up to 10,000 microg/plate and did no ... | 2009 | 19631708 |
| kefiran protects caco-2 cells from cytopathic effects induced by bacillus cereus infection. | the aim of this work was to evaluate the ability of kefiran to antagonize cytopathic effects triggered by bacillus cereus strain b10502 on cultured human enterocytes (caco-2 cells). cell damage was evaluated by f-actin labelling, scanning electron microscopy and determination of ratios of necrotic and detached cells. to assess the interaction between kefiran and bacteria or eukaryotic cells, flow cytometric analysis was conducted with fitc-labelled kefiran. kefiran significantly protected infect ... | 2009 | 19633916 |