Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [posterior hernia of a calcified disk in children. apropos of 2 cases]. | posterior herniation of a calcified disk was detected in two children. a 10-year-old boy presented with cervicobrachial neuralgia at the c7 level secondary to herniation of a calcified c6-c7 disk treated by surgery. a 13-year-old boy was seen with a post-traumatic painful stiffness in the thoracic region posteriorly, without neurologic complication, secondary to a herniated calcified t4-t5 disk. surgery was not performed and the course was favorable with regression of clinical signs and spontane ... | 1985 | 4087235 |
| thyroid hormones modulate the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd). | these experiments examine the role of thyroxine (t4) and triiodothyronine (t3) on the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd). the first experiment is continuation of a study reported previously (rozman et al., 1984). in this experiment, 60 male sprague-dawley rats were divided into 6 equal groups. four groups of rats were thyroidectomized by 3 mci na131 l/kg rat. five weeks later 2 of the thyroidectomized and 1 of the nonthyroidectomized groups of rats received ip 100 micrograms ... | 1985 | 4087313 |
| [clinical study of spinal intradural arachnoid cyst]. | spinal intradural arachnoid cysts (s-iac) are rarely found and only 80 cases have been reported since 1915. we report two of them. a 68-year-old male had slowly progressive dull pain in both thighs for about 6 years prior to admission. neurological examination and plain x-ray on admission showed no abnormality. myodil myelography showed a s-iac at t4 vertebral level. surgical treatment of cyst wall excision resulted in success. the other case, a 39-year-old male suddenly noticed monoparesis and ... | 1985 | 4088443 |
| [functional study of the hypophyseal-thyroid and adrenal systems in physiological pregnancy and late toxicosis]. | a comprehensive study was conducted in 30 healthy nonpregnant women, 115 healthy pregnant women and in 38 pregnant women with late toxicosis as well as in 25 healthy infants and 29 infants born to mothers with late toxicosis. a radioimmunoassay was used to measure serum thyrotropin (tsh), somatotropin (sth), adrenocorticotropin (acth), thyroxine (t4), triiodothyronine (t3), cortisol (cort) and aldosterone (aldo) levels. a catecholamine (ca) level was determined dy fluorimetry. elevated acth, tsh ... | 1985 | 4088984 |
| [changes in thyroid hormone status after injections of iodinated contrast media]. | administration of four different contrast media exerted slight but significant and prolonged effects on the hormonal thyroid status, in 53 euthyroid subjects (four subgroups), without any clinical expression of dysthyroidism. changes in serum t3, rt3 and t4 demonstrated different trends for each iodinated compound, probably depending on the regulatory thyroid mechanisms, and cm pharmacokinetics. plasma profiles of free fractions (ft3, ft4) slightly reflected consensual changes with circulating i ... | 1985 | 4089260 |
| immediate reconstruction using visceral transfer after pharyngolaryngoesophagectomy for advanced hypopharyngeal cancer. | the management of 32 stage iv cases of cancer of the hypopharynx was reviewed retrospectively with a view of selecting a pertinent treatment modality to ensure low morbidity and high survival and evaluating the applicability of abdominal viscera as a reconstructive measure after total pharyngolaryngoesophagectomy. three methods of visceral reconstruction have been used at this hospital, viz., reversed gastric tube, colon interposition, and stomach transposition. in our experience, the gastric pu ... | 1985 | 4089393 |
| activated t lymphocytes of the synovial membrane in rheumatoid arthritis and other arthropathies. | immunohistological techniques were used to identify activated t lymphocytes within the synovial membrane of patients with rheumatoid arthritis, using the monoclonal antibody (moab) rft2, which identifies a 40-k dalton molecule preferentially expressed by t blasts or activated cells. using this reagent together with a monoclonal 'cocktail' that stains all t cells, cell counts on consecutive sections of rheumatoid synovium revealed that up to 50% t lymphocytes were rft2+ (range 9.3-50.2%, mean 25. ... | 1985 | 4089540 |
| [ureterotransversopyelostomy with unilateral nephrostomy]. | supravesical urinary diversion by ureterotransversopyelostomy (utps) with unilateral nephrostomy was performed in 57 patients. the age of the 33 women ranged between 42 and 86 (mean 65), of the 24 men between 39 and 77 (mean 62) years. with a single exception, the indication for diversion was palliative: 25 patients had advanced bladder cancer (t3/t4), and 19 had undergone irradiation; 24 patients showed vesico- (recto-) vaginal fistulas due to radiation for gynecological carcinomas. in 2 patien ... | 1985 | 4090130 |
| the bioavailability of thyroxine and 3,5,3'-triiodothyronine in normal subjects and in hyper- and hypothyroid patients. | a new method for the estimation of the bioavailability of thyroxine (t4) and 3,5,3'-triiodothyronine (t3) is described based on gel separation followed by antibody extraction of labelled t4 and t3 from serum, and using the area under the curve of disappearance of the tracer (auc) for the calculations. the peak serum concentrations of radioactive labelled t4 and t3 were reached approximately 90 min after oral administration of both tracers. the relative difference of duplicate estimations was bel ... | 1985 | 4090911 |
| how to deal with undetectable and low measurable serum thyroglobulin levels in the follow-up of patients with differentiated thyroid carcinoma? | serum thyroglobulin (tg) was measured in 52 patients 3 months to 15 years (mean 5.3 years) after thyroidectomy with or without subsequent radioablation for differentiated thyroid carcinoma, before and after the interruption of suppressive thyroxine (t4) replacement therapy for 5 weeks. whole body scintigraphy was carried out at the end of the t4 withdrawal period. serum tg was undetectable (less than 3 micrograms/l) in 38 patients on t4 therapy, in 18 the scintigraphy showed a minimal accumulati ... | 1985 | 4090912 |
| [malpighian cancer of the anal canal in côte-d'or. epidemiological and therapeutic aspects]. | the registry of digestive tract tumours established for the department of côte-d'or was used to study the incidence and the treatment of squamous cell cancer of the anal canal. in the course of 7 years (1976-1982), 45 new cases of anal canal cancer were recorded. age standardized rates using world standard population are 1.3/100,000 for females, 0.3/100,000 for males. as compared to other cancer registries, côte-d'or is in the high range for females, in the average range for males, the cases inc ... | 1985 | 4092101 |
| [choice of the treatment for lip carcinoma--an analysis on 74 cases]. | this paper reports the results of the treatment of 74 patients with lip carcinoma from 1958-1974. all patients were proved by pathology and received their first course of treatment in our hospital. 44 of these patients were treated by radiation and 30, by surgery. the over-all five-year survival rate is 59% (44/74). satisfactory results were obtained in the early lesions (stages i, ii) either by surgery or radiation and the five-year survival rates are 66% (surgery 6/9, radiation 4/6). for stage ... | 1985 | 4092579 |
| manganese ion as a goitrogen in the female mouse. | effect of excessive ingestion of manganese (mn) on the mouse thyroid was assessed under the conditions of normal intake of iodide. female mouse thyroids were enlarged after 7 weeks of administration of 200 mg/l mncl2 x 4h2o in drinking water; 2.74 +/- 0.25 mg for control (n = 56), and 3.31 +/- 0.28 mg for mn-treated group (n = 85) (p less than 0.001). in contrast, male mouse thyroids never became goitrous following this treatment. manganese was goitrogenic to the castrated male mouse, but it had ... | 1985 | 4092670 |
| lack of effects of thyroid hormones on human erythrocyte acetylcholine esterase. | effects of thyroid hormones and their metabolites such as l-t1, l-t2, l-t3 and l-t4 on human erythrocyte acetylcholine esterase were studied. the activity of the enzyme of intact erythrocytes was not affected by these hormones, though studied under various conditions. the physiological significance of the binding of these hormones to erythrocyte membranes remains unclear. our results indicate that the acetylcholine esterase is not a suitable enzyme for cytochemical bioassay for thyroid hormones. | 1985 | 4092676 |
| [induction of cytosolic proteins controlling mitochondrial protein synthesis by thyroid hormone]. | the effect of thyroid hormone on the generation of modulators of mitochondrial protein synthesis was investigated. the modulators were present in the 150,000 x g supernatant (s-150) prepared from wistar rat liver and kidney. a stimulator was found in the liver, and an inhibitory modulator was present in kidney tissue. generation of these modulators was stimulated by t4 administration to the animals. both the stimulatory and inhibitory modulators were inactivated by pretreatment with trypsin or b ... | 1985 | 4092784 |
| cryo-electron microscopy of helical particles tmv and t4 polyheads. | vitrified tobacco mosaic virus (tmv) and t4 polyhead suspensions have been studied by cryo-electron microscopy. the order of tmv particles is preserved to distances better than 0.3 nm when vitrified. in spite of the high beam sensitivity of unstained biological material, the resolution of images extends to 1.15 nm. radial density distributions calculated from images of tmv and the helical aggregate of tmv protein (tmvp) show that the rna is likely visualized in the virus. however, due to the ima ... | 1985 | 4093967 |
| structure-function studies of the bacteriophage t4 dna polymerase. isolation of a novel suppressor mutant. | we describe here our first attempt in using suppressor mutations to study structure-function relationships of the bacteriophage t4 dna polymerase. one intragenic suppressor mutation, j5(43) degrees, was isolated that suppresses the temperature sensitivity but not the mutator activity of tsm19, a dna polymerase mutant. thus, the substituted amino acid induced by the tsm19 lesion decreases dna polymerase fidelity, even if the temperature sensitivity has been corrected by a second amino acid substi ... | 1985 | 4093978 |
| combined modality treatment with initial chemotherapy in t4 and/or n2-n3 primary breast cancer. | 1985 | 4095120 | |
| [repercussion of the application of povidone-iodine to the umbilical stump on thyroid function of the neonate at term]. | we have studied blood tsh and t4 5-7 days after birth in 356-healthy newborns whose umbilical stump had been treated with 10% povidone-iodine. the prevalence of high tsh levels (above 20 microu/ml) was higher in this group than in the general population (3.09% vs 0.417%, p less than 0.001), and so was the rate of transient hypothyroidism (2.73% vs 0.25%, p less than 0.001). both differences were related to the number of applications of iodine given. all children were found to be normal at retest ... | 1985 | 4096451 |
| acquired immunodeficiency syndrome (aids) in infants and children: report of nine cases. | we report here nine children with aids. the risk factors of these patients were hemophilia in one, blood transfusions in four, maternal intravenous drug use in three and paternal aids in one. one baby was also of haitian parentage. the major clinical symptoms included failure to thrive, hepatomegaly, lymphadenopathy, interstitial pneumonia, recurrent bacterial and viral infections and persistent oral thrush. three infants had chronic recurrent parotitis. five infants developed opportunistic infe ... | 1985 | 3830264 |
| thyroid function in type 1 juvenile diabetes mellitus: tendency to the low t3 syndrome. | we have evaluated the thyroid function in 30 children and adolescents (average age 13 2/12 +/- 3 10/12 years) suffering from type 1 insulin-dependent diabetes (average duration of illness 5 +/- 3 3/12 years). we divided them into group a (good control) and group b (poor control), according to whether they had presented an hba1c value lower or higher than 10%. the results obtained have been compared with a group of 30 normal patients of the same age. both groups of diabetics presented t4 values w ... | 1985 | 3830971 |
| pineal-induced depression of free thyroxine in syrian hamsters. | blind hamsters had no alteration of the dialyzable fraction of serum thyroxine (t4) but had depressed total and free t4 concentrations compared to controls. prevention of the effects of blinding by pinealectomy indicates pineal influence on circulating free t4 concentration. parallel changes in free t4 and the free t4 index indicate adequacy of the index in representing pineal-induced changes in free t4. | 1985 | 3831316 |
| the substrate specificity, tissue specificity and regulation of the 5' deiodination systems in rat liver and kidney tissues. | studies were carried out to compare the 5' deiodination reactions of thyroxine (t4) and 3, 3', 5'-triiodothyronine (rt3) in rat liver and kidney homogenates. the 5'-deiodinase activity was assayed by the 3, 5, 3'-triiodothyronine (t3) produced from t4 or by the 125i-iodide released from 125i-rt3. the two 5' deiodination reactions had similar ranges of optimal ph, incubation temperature, and apparent km, t4 1.1 and rt3 1.3 microm. however, the apparent vmax values for t4 and rt3 deiodination reac ... | 1985 | 3833526 |
| changes in somatomedin activity in anorexia nervosa. | somatomedin (sm) activity, gh, t3 and t4 were investigated in 6 girls with anorexia nervosa during hospitalization and at outpatient clinic. on admission, serum t3 (27-62 ng/dl) and sm activity (0.24-0.55 u/ml) were low in all cases, while basal gh was extremely high in 2 cases. a significant negative correlation was found between sm activity and basal gh during the course of treatment (r = -0.61, p less than 0.02). the change in sm activity was related to that of the serum t3 level and a signif ... | 1985 | 3833530 |
| comparison of a new ultrafiltration method for serum free t4 and free t3 with two ria kits in eight groups of patients. | we measured serum free thyroxine (ft4) and free t3 (ft3) concentrations by a newly developed ultrafiltration (uf) method and by the diagnostic products corporation (dpc) and amersham corporation (amerlex) ria methods in 173 patients divided into eight groups: 1) normal controls, ii) normal elderly subjects, iii) hyperthyroidism, iv) hypothyroidism, v) pregnancy, vi) critically ill, vii) chronic renal failure, and viii) heparin therapy. the results were also compared with the free t4 index and fr ... | 1985 | 3833894 |
| evaluation of pituitary-thyroid axis response to acute myocardial infarction. | we have studied with seriated controls for a period of 9 days 18 patients admitted to our hospital for acute myocardial infarction (ami). slight, but non significant variations in thyroidal hormone pattern were observed: slight decrease of t3 and t4 levels, increase of reverse t3 on day 3, low free t4 levels, slight increase of tsh levels until the 3rd day. however, hormonal pattern was clearly different in patients who presented a clinical improvement (group 1a) and in patients who died for ami ... | 1985 | 3833895 |
| is conversion of thyroxine to triiodothyronine and reverse triiodothyronine autoregulated? studies with short term suppression of thyroid function with 3'isopropyl 3,5-diiodothyronine. | we investigated the effect of a 6 days course of 20 micrograms of a thyromimetic thyronine analogue 3'isopropyl, 3,5-diiodothyronine (diip) on serum thyroid hormone and tsh levels in 10 normal male volunteers. tsh was inhibited progressively during the 6 days of diip treatment. by the third day after stopping the treatment this trend had already reversed. of the serum thyroid hormones the t3 level had decreased by 32% and was the first to increase 5 days after stopping treatment. serum t4 fell b ... | 1985 | 3833896 |
| influence of the neonatal suppression of tsh production (neonatal hyperthyroidism) on response to tsh in adulthood. | six-day treatment of neonatal rats with t3 or t4 resulted in a considerable depression of t4 production in response to exogenous tsh in adulthood. this supports the hypothetical conclusion that hormonal imprinting, i.e. the presence of the hormone and its interaction with the cell during the period of receptor maturation, is important for receptor amplification, which accounts for normal response to the hormone in adulthood. | 1985 | 3833900 |
| posterior fusion for idiopathic scoliosis. | a posterior fusion is the standard operative approach that will allow the orthopaedic surgeon to treat most scoliosis satisfactorily. the extent of fusion should be determined by the type and extent of the curve. all spines should not be fused from t4 to l4. the management of the blood loss has evolved to a very controlled situation, with the reasonable goal that no patient should be sensitized to homologous blood. instrumentation systems provide good correction and stability to assist in provid ... | 1985 | 3833935 |
| the pathologic response of the liver and thyroid of the rat to potassium prorenoate (sc-23992). | a 3-month dose range finding study in preparation for a 2-yr carcinogenicity study of potassium prorenoate (sc-23992), a steroid with an antihypertensive profile, is reported. the drug was administered by gavage once daily at doses of 10, 30, and 100 mg/kg/day to charles river cd rats. treatment was terminated at 13 weeks and 10 randomly selected animals from each treatment group were killed and necropsied. the remaining 10 animals in each dose group, including controls, were maintained for an a ... | 1985 | 3834584 |
| [changes in free and total thyroid hormones and reverse t3 in adult male rats during fasting]. | the serum levels of thyroid hormones (t3, f.t3, rt3, t4 and f.t4), cholesterol and triglycerides were measured in a population of adult male rats subjected to experimental fasting. comparison of bindings with results published in the literature reveals certain disagreements attributable to individual variations in the animals resulting from physical or physiological factors. this study demonstrates that deiodination of thyroxine in t3-reverse has an economic role on a metabolic level. evaluation ... | 1985 | 3836591 |
| [thymus-hypophysis-thyroid interrelation in rats after administration of a heterologous extract of thymus gland]. | the authors have investigated the effects of a thymus chromatographic fraction on tsh, t3, t4 serum values in thyroidectomized and normal controls rats before and after thymus treatment. the decrease in tsh values of thyroidectomized rats points out a possible inhibitory effect which the thymus extract may have at either the level of the adenohypophysis or hypothalamus. moreover the tsh values in the euthyroid rats after thymus treatment showed a slight decrease, this changes are much more evide ... | 1985 | 3836592 |
| a proposal of tnm classification system for cancer of the salivary gland--comprehensive retrospective study. | until recently, there has not been a sufficient number of cases of salivary gland cancer to permit the development of a clinically useful tnm classification system. a comprehensive retrospective study of 354 patients with cancer of the parotid gland from 46 institutions in japan was carried out at the request of the japanese committee on tnm classification (jjc), and we propose jjc t-categories for parotid gland cancer. the characteristic of this proposal involves t-categories determined accordi ... | 1985 | 3836630 |
| analysis of the long-term results of our combination therapy for maxillary cancer, with particular reference to treatment of t4 and n1-3 maxillary cancer. | to evaluate how far the long-term survival rate could be improved by maximum combination of chemotherapy, radiotherapy, and surgery, we have performed a trial combination therapy consisting of linac x-ray irradiation (200 rads x 30) and intraarterial infusion chemotherapy (5-fluorouracil 250 mg x 15), followed by sufficiently extensive maxillectomy and primary reconstruction. the 5-year crude and relative survival rate of 70 patients were 64.2 and 72.1% respectively. the 5-year crude survival ra ... | 1985 | 3836633 |
| surgery for squamous cell carcinoma of the tongue and floor of the mouth. | surgery for cancer of the tongue and floor of the mouth has become more varied and generally more conservative, influenced by advances in oncology and modern reconstructive methods. combined therapy is favored, with postoperative irradiation and sometimes adjunctive chemotherapy, using cis-platinum. t1 carcinomas of the tongue and floor of the mouth can be treated with either wide local excision or irradiation alone, but surgery is the preferred method. t2-t4 tumors treated by resection combined ... | 1985 | 3836654 |
| dissection of parapharyngeal space in head and neck cancer. | the parapharyngeal space is a potential space located lateral to the upper pharynx and tonsillar area. this space can be involved either by direct extension, by perineural or neural spread, or by lymph node metastasis from cancers originating in adjacent sites. between 1978 and 1984, 22 patients with t3 or t4 carcinoma of the head and neck region underwent dissection of the parapharyngeal space in conjunction with ablation of the primary tumor and standard radical neck dissection. twelve of thes ... | 1985 | 3836656 |
| [preliminary observation on changes in serum triiodothyronine (t8), thyroxine (t4) nd thyroid-stimulating hormone (tsh) in patients with chronic cor pulmonale]. | 1985 | 3837364 | |
| thyroid hormone binding to the rat heart cytosol proteins. | the properties of the thyroid hormone binding to rat heart cytosol were studied. cytosol proteins were found to bind specifically t4 with high affinity (ka approximately equal to 10(8)m-1) and rt3 with lower affinity (ka approximately equal to 10(7)m-1), but they do not bind t3. the binding of both t4 and rt3 was ph dependent, however, while t4 binding had the highest values between ph 7.0 and 10, rt3 binding increased from ph 6.0 to 10.7. divalent ions also stimulated t4 and rt3 binding. sulfhy ... | 1985 | 3837981 |
| a comparison of the effects of propylthiouracil and methimazol on circulating thyroid hormones and various measures of peripheral thyroid hormone effects in thyrotoxic patients. | two groups of patients with newly diagnosed thyrotoxicosis were treated with propylthiouracil (ptu) 400 mg every 6 h for 4 days followed by methimazol (mmi) 40 mg every 6 h for 4 days or by mmi for 4 days followed by ptu for 4 days. the shift from mmi to ptu induced a considerable decrease in serum t3 while shift from ptu to mmi led to an increase in serum t3. serum t4 decreased gradually during the whole treatment period. the opposite variations in serum t3 were accompanied by similar opposite ... | 1985 | 3838216 |
| comparative effects of sodium ipodate and iodide on serum thyroid hormone concentrations in patients with graves' disease. | patients with thyrotoxic graves' disease were treated daily for 10 d with 1 g sodium ipodate, an iodine rich x-ray contrast agent which impairs outer ring (5'-) deiodination of t4 to t3, or with 12 drops of a saturated solution of potassium iodide (sski). t4, t3 and reverse t3 (rt3) concentrations were measured before, during, and 5 and 10 d after the administration of each drug. sski therapy induced a decrease in the serum t4 concentration from 14.7 +/- 1.3 microgram/dl (mean +/- se) to a nadir ... | 1985 | 3838710 |
| coupling of iodotyrosine catalyzed by human thyroid peroxidase in vitro. | the coupling of iodotyrosine (coupling reaction) is one of the least studied in the formation of thyroid hormone, particularly in human thyroid diseases. this paper describes a method of measuring iodotyrosine coupling catalyzed by human thyroid peroxidase (tpo) in vitro. there were two important requirements to demonstrate the coupling reaction: 1) thyroglobulin with a low thyroid hormone content, and 2) partially purified tpo. thyroglobulin with low thyroid hormone content was obtained from gr ... | 1985 | 3838997 |
| microinjection of micrococcus luteus uv-endonuclease restores uv-induced unscheduled dna synthesis in cells of 9 xeroderma pigmentosum complementation groups. | the uv-induced unscheduled dna synthesis (uds) in cultured cells of excision-deficient xeroderma pigmentosum (xp) complementation groups a through i was assayed after injection of micrococcus luteus uv-endonuclease using glass microneedles. in all complementation groups a restoration of the uv-induced uds, in some cells to the repair-proficient human level, was observed. another prokaryotic dna-repair enzyme, t4 endonuclease v, restored the uv-induced uds in a similar way after microinjection in ... | 1985 | 3839045 |
| environmental iodine intake affects the response to methimazole in patients with diffuse toxic goiter. | the response to methimazole [1-methyl-2-mercaptoimidazole (mmi)] therapy was evaluated in 18 patients with diffuse toxic goiter residing in an area of iodine deficiency (tehran) and in 18 patients residing in an area of iodine sufficiency (boston). the mean free t4 index (ft4i) decreased from 22.9 +/- 4.8 (+/- sd) to 4.9 +/- 4.3 in tehran and from 23.8 +/- 5.2 to 17.0 +/- 4.1 in boston after 4 weeks of mmi administration (10 mg, three times daily). the mean free t3 index (ft3i) decreased from 48 ... | 1985 | 3839244 |
| [anti-triiodothyronine antibodies in patients with rheumatoid arthritis associated with hashimoto's thyroiditis]. | anti-triiodothyronine antibody was found in a case of rheumatoid arthritis associated with hashimoto's thyroiditis. the patient was a 40 year-old woman who had complained of polyarthralgia, joint-swelling and stiffness for seven years. she had a rheumatoid nodule and showed a positive ra test. radiographic changes of hands and wrists showed osteoporosis, erosions and narrowing of joint space. nonsteroidal anti-inflammatory drugs had been used for seven years. the diagnosis of hashimoto's thyroid ... | 1985 | 3839465 |
| familial "hashitoxic' periodic paralysis. | four members of a chinese family who had thyrotoxicosis and periodic paralysis are described. two of these patients had "hashitoxicosis' (graves' disease and hashimoto's thyroiditis) as evidenced by the presence of thyroid antibodies in addition to elevated thyroxine (t4) levels. the other two patients were not available for testing. the association of the familial occurrence of "hashitoxicosis' and periodic paralysis does not appear to have been reported previously. | 1985 | 3839536 |
| serum and thyroid hormones t3 and t4 regulate nerve growth factor mrna levels in mouse l cells. | mouse l cells synthesize and secrete a neurotrophic factor related to the beta subunit of the submaxillary gland nerve growth factor (ngf) of male mice. use of a cdna probe which encodes the beta-ngf mrna demonstrated that l cells produce a transcript identical in size to that of the submaxillary gland. moreover, target sites of restriction enzymes ecori, psti and bamhi were not significantly rearranged in the beta-ngf gene locus of these cells. the abundance of the beta-ngf transcript was found ... | 1985 | 3839757 |
| computer simulation for hormones related to primary thyropathy. | we propose a mathematical model of the human hypothalamus-anterior pituitary-thyroid system regulating basal metabolism, and practice computer simulation concerning primary thyropathy such as graves' disease, hypothyroidism, t4-toxicosis and t3-toxicosis by use of this model. in order to throw light on properties of the system, indicial responses of the hormones, t4, t3, rt3, and tsh, and the function of the thyroid gland are computed. medical treatments for graves' disease and for hypothyroidis ... | 1985 | 3840040 |
| [diffuse toxic goiter associated with autoimmune thyroiditis]. | a study of 190 patients with diffuse toxic goiter combined with autoimmune thyroiditis has revealed some clinical features: a torpid course and a solid-elastic consistency of the thyroid. in 37.4% of the cases autoimmune thyroiditis was combined with a severe form of thyrotoxicosis. the combined form of diffuse toxic goiter correlated with a greater frequency of the detection of thyroid autoantibodies to thyroglobulin and microsomal antigen (in 83 and 78.3% of the patients respectively) as compa ... | 1985 | 3840258 |
| multicentric giant lymph node hyperplasia, kaposi's sarcoma, and lymphoma. | we studied two cases of a recently recognized systemic lymphoproliferative disease with morphological features of castleman's disease: multicentric giant lymph node hyperplasia. both patients developed kaposi's sarcoma and had laboratory evidence of immune abnormalities, including reversed t4 to t8 ratios. one patient's disease had a subacute course with fevers of unknown origin, mucocutaneous candidiasis, and progressive thrombocytopenia, while the other patient's disease had a fulminant course ... | 1985 | 3840353 |
| clinical experience with sensitive thyrotropin measurements: diagnostic and therapeutic implications. | a two-site immunoradiometric assay for serum thyrotropin (tsh) was modified to improve the analytical sensitivity. the sensitivity achieved (detection limit, approximately 0.1 microu/ml; lower limit of quantitative measurement, approximately 0.4 microu/ml) was comparable to that of the best competitive binding research assays, yet this assay can be performed routinely. serum tsh was 1.82 +/- 0.69 (mean +/- s.d.) (range 0.4-3.4 microu/ml) in healthy individuals and 1.83 +/- 0.90 microu/ml (range ... | 1985 | 3840528 |
| the clinical and metabolic responses to early surgical treatment for hyperthyroid graves' disease: a comparison of three pre-operative treatment regimens. | we have compared the clinical and metabolic responses of three groups of patients undergoing subtotal thyroidectomy for hyperthyroid graves' disease. these patients were prepared for surgery with propranolol alone, propranolol plus potassium iodide or the long acting beta-adrenoceptor antagonist nadolol plus potassium iodide. pre-treatment with potassium iodide reduced serum t4 to normal or subnormal in all patients. patients in the propranolol group had significantly higher pulse rates and temp ... | 1985 | 3840600 |
| a simulation of t4 bacteriophage assembly and operation. | a model is presented for the self-assembly and operation of a bacteriophage comparable with the t4 bacteriophage that infects escherichia coli. the model treats protein molecules as simple units obeying the principle free energy minimization, and exhibiting the properties of quasi-equivalence and conformational switching. a computer program incorporating the model has been developed. the results of simulation using this program are presented. | 1985 | 3840704 |
| [changes in the immune status indices of patients with diffuse toxic goiter in different stages of the disease]. | the purpose was to study some indices of immunity in the period of mercazolyl therapy and during dtg remission. t4, t3, tsh and some indices of the immunological status: rfcc, rfcl, rfcn, stimulation index (si) in the lymphocyte blast transformation reaction, serum immunoglobulins (ig a, m, g) were studied in 44 patients. in diffuse toxic goiter (dtg) rfcc, rfcl and rfcn were two times and more as less, the ig g level was raised 1.5-fold. mercazolyl therapy corrected rfcc, rfcn whereas rfcl rema ... | 1985 | 3841395 |
| homeorhesis during early lactation. euthyroid sick-like syndrome in lactating cows. | serum levels of thyroxine (t4), triiodothyronine (t3) and reverse t3 (rt3) were studied in dry, early (first trimester) and middle (second trimester) lactating purebred holstein cows. the study encompassed three different seasons: autumn, winter and spring. under comfortable weather conditions (temperature 22 degrees c; relative humidity, 40%) or moderately hot (28 degrees c; 60%), january and october, respectively, cows in early lactation exhibited significantly lower levels of t4 and t3, and h ... | 1985 | 3841446 |
| monoclonal antibody patterns in lymphomatoid papulosis. | atypical cells resembling reed-sternberg cells are a characteristic histologic feature of lymphomatoid papulosis. thus far no consistent data are available on the nature of these cells, or a possible antigenic relationship between them and reed-sternberg cells. twenty-four biopsy specimens from 14 patients with lymphomatoid papulosis were immunolabeled with antibodies against ki-1 and other reed-sternberg cell-associated antigens. in all cases a proportion of the large, atypical cells expressed ... | 1985 | 3842652 |
| primary structure of bovine thyroglobulin deduced from the sequence of its 8,431-base complementary dna. | in mammals, an adequate supply of thyroid hormones is essential for normal growth and neurological development. the biosynthesis of thyroid hormones involves an iodinated precursor protein, thyroglobulin, which may be considered an extreme example of a pro-hormone. thyroglobulin is a dimeric glycoprotein of relative molecular mass (mr) 660,000 (660k), which is secreted by the thyrocyte and stored in the lumen of the thyroid follicle. the hormonogenic reaction is extracellular, and involves iodin ... | 1985 | 3855243 |
| thyroid dysfunction and neoplasia in children receiving neck irradiation for cancer. | the reported relationship of radiation exposure and thyroid carcinoma stimulated this retrospective study of 298 patients treated at st. jude children's hospital with radiation therapy to the neck for childhood cancer to identify patients who developed subsequent thyroid abnormalities. this series includes 153 patients with hodgkin's disease, 95 with acute lymphocytic leukemia, 28 with lymphoepithelioma, and 22 with miscellaneous tumors. inclusion in the study required 5 years of disease-free su ... | 1985 | 3855682 |
| a genetic screen for mutations that increase the thermal stability of phage t4 lysozyme. | a method has been developed to screen for mutants of phage t4 lysozyme that are more stable than the wild-type enzyme. using an assay that detects lysozyme activity on petri plates [streisinger, g., okada, y., emrich, j., newton, j., tsugita, a., terzaghi, e. & inouye, m. (1966) cold spring harbor symp. quant. biol. 31, 77-84], protein synthesized during the formation of phage plaques at a permissive temperature (33 degrees c) was tested for its ability to withstand incubation at a temperature t ... | 1985 | 3856227 |
| immunologic alterations in monkeys with simian acquired immunodeficiency syndrome (saids). | levels of lymphocyte responsiveness to t- and b-cell-specific mitogens and expressions of ia, t4, t8, and t11 surface markers were monitored during the course of simian acquired immunodeficiency syndrome (saids) in four rhesus macaques that either died or became ill and survived. the monkey that died showed progressively suppressed responses to concanavalin a (con a), phytohemagglutinin (pha), pokeweed mitogen (pwm), and staphylococcus aureus cowan i strain (sac) through the time of death (5 1/2 ... | 1985 | 3857635 |
| [combination effects of cddp, acr and hcfu on progressive urothelial tumors]. | nine patients with progressive urothelial tumors treated with combination chemotherapy were evaluated. the median age of the patients was fifty-four; 6 men and 3 women; 7 of the tumors were of the renal pelvis and/or ureter and at stage c or d at the time of initial chemotherapy. another of the tumors was triple cancer (bladder, prostate, sigmoid) and the remaining case was of bladder cancer. the stage of the two bladders cancers was t4. all patients were treated with cddp (cis-platinum), acr (a ... | 1985 | 3859251 |
| interaction between intestinal and peripheral blood mononuclear cells. | effective methods are available to isolated immunocytes from human intestinal mucosa. isolated mononuclear cells from the mucosa of patients with crohn's disease and normal controls were classified according to ultrastructure and cell-surface profile by the use of a panel of monoclonal antibodies. in both compartments of the mucosa, i.e., the lamina propria and the epithelial layer, no differences were found between intestinal tissue from control patients and unaffected mucosa of patients with c ... | 1985 | 3861037 |
| perturbation of maintenance and de novo dna methylation in vitro by uvb (280-340 nm)-induced pyrimidine photodimers. | the effect of pyrimidine photodimers on transmethylation reactions catalyzed by a highly purified rat liver dna (cytosine-5-)-methyltransferase (ec 2.1.1.37) that exhibits maintenance and de novo methylation activities was studied in vitro, using the viral substrates m13 mp9 replicative form (rf) dna and the hemimethylated analog formed from primed synthesis of phage dna in the presence of 2'-deoxy-5-methylcytidine 5'-triphosphate. these dnas were irradiated with uvb (280-340 nm) at 900-3600 j/m ... | 1985 | 3862117 |
| multiple class i and class ii major histocompatibility complex allospecificities are generated with t cell receptor variable (v) domains created by a single ti beta v gene family. | 10 alloreactive cytotoxic t lymphocytes using rex ti beta variable region (v) gene segments in formation of their antigen/major histocompatibility complex (mhc) t3-ti receptor were selected, cloned, and characterized in an effort to examine the extent of receptor diversity created by this one v gene family. multiple and distinct class ii as well as class i allospecificities were generated from the formation of different ti beta v domains. five allospecificities were directed at various class i e ... | 1985 | 3862747 |
| genetic complementation of uv-induced dna repair in chinese hamster ovary cells by the denv gene of phage t4. | the denv gene of phage t4, encoding the pyrimidine dimer-specific dna repair enzyme endonuclease v, has been introduced by dna transfection into the uv-sensitive dna repair-deficient chinese hamster ovary (cho) cell line uv5. transformants were first selected for resistance to the antibiotic g418 conferred by the neo gene from tn5 carried by the same plasmid. a majority of the isolated g418-resistant uv5 clones also showed an increased resistance to 254-nm uv light. one clone, designated i-a1, w ... | 1985 | 3865186 |
| ion etching bacteriophage t4: support for a spiral-fold model of packaged dna. | ion etching of bacteriophage t4 erodes virus components progressively from the outside to the inside while preserving the overall structure. the terminal portion of the t4 dna molecule packaged can be specifically radiolabeled and was found to be eroded more rapidly than the remainder of the dna. this strongly suggests that the first dna to enter the prohead is condensed in the center of the capsid and is therefore shielded from the ion beam by the surrounding last packaged dna. the results supp ... | 1985 | 3865208 |
| alterations in vitamin a and thyroid hormone status in anorexia nervosa and associated disorders. | vitamin a and thyroid hormone status was investigated in 27 patients with anorexia nervosa. subjects were divided into three groups based on eating behavior and serum carotene concentrations: anorexic (dietary restriction), normal carotene; anorexic, elevated serum carotene; bulimic, elevated serum carotene. all bulimic subjects fulfilling selection criteria were hypercarotenemic (weight loss and reduced metabolic rate). data were compared to normal healthy volunteers. serum retinol and retinol- ... | 1985 | 3865529 |
| lack of maternal metabolic, endocrine, and environmental influences in the etiology of cleft lip with or without cleft palate. | we studied metabolic, endocrine, and environmental factors in 59 women who had delivered a child with cleft lip with or without cleft palate (cl +/- cp) and compared these values with those of 56 mothers of unaffected children. there was no significant difference between the two groups with respect to race, age, weight, height, education, parity, menstrual history, medical illnesses, or the use of contraceptives, tobacco, alcohol, or caffeine. all patients had a normal xx karyotype confirmed by ... | 1985 | 3868431 |
| a study on antisperm antibody in homosexual men. | using a hemagglutination assay we surveyed antibody distribution to human sperm antigen in a sexually transmitted disease (std) clinic population and in an aids study group. we found no significant difference for the mean antisperm antibody titres between sexually active heterosexual men, women or homosexual men, homosexual men with or without aids, and no correlation between t4/t8 lymphocyte ratios and antibody titres in homosexual men. we conclude that levels of antibody to sperm antigens are ... | 1985 | 3868684 |
| plasma concentrations of thyroid hormones in dogs: influence of sampling hour, breed and age. | thyroxine (t4) and triiodothyronine (t3) plasma concentrations have been determined during 24-hr sampling periods in six mongrels (age 12-36 months), six beagles (age 35-37 months), three labradors (age 3.5 months) and three beagles (age 5 months). the mean t4 levels of the labradors were significantly lower than the values found for mongrels or older beagles (p less than 0.05), whereas t3 was higher in the 5 month old beagles compared to the mongrels (p less than 0.001), young beagles (p less t ... | 1985 | 3870842 |
| murine anti-swine t4 and t8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic t cells. | two murine monoclonal antibodies reactive with porcine nylon wool-nonadherent t cells were examined by flow microfluorometry analysis for cellular distribution, and by functional analysis for their effect on mlr and ctl assays. the two determinants recognized, called pt4 and pt8, were shown to be expressed on almost mutually exclusive cell populations, with only 6 to 15% of t cells expressing both antigens. among the 15% of nylon wool-nonadherent cells that did not express either pt4 or pt8, 5% ... | 1985 | 3871107 |
| identification and characterization of a b cell growth inhibitory factor (bif) on bcgf-dependent b cell proliferation. | the culture supernatants of con a-activated human peripheral blood mononuclear cells (pbm) contained at least two regulatory factors upon b cell proliferation. one was b cell growth factor (bcgf), which activated antigen-stimulated b cells to proliferation and clonal expansion, and the other was its inhibitory factor, arbitrarily named b cell growth inhibitory factor (bif). this bif inhibited the effect of bcgf on anti-mu-stimulated b cells or the monoclonal mature b cell line (cll-t.h.) obtaine ... | 1985 | 3871108 |
| plain-film criteria for excluding aortic rupture in blunt chest trauma. | emergency room supine chest films of 86 consecutive patients with blunt chest trauma and possible aortic rupture were reviewed. sixteen radiographic signs were analyzed independently and in combination. only two signs associated with aortic rupture were statistically significant: deviation of the nasogastric tube to the right at the t4 level and depression of the left main-stem bronchus below 40 degrees from the horizontal. false positives and false negatives occurred with each individual radiog ... | 1985 | 3871138 |
| t cell chronic lymphocytic leukemia with lymphocytes of unusual immunologic phenotype and function. | this report discusses a case of t cell chronic lymphocytic leukemia (t-cll) in an elderly white man whose lymphocytes expressed a post-thymic phenotype except for the coexpression of t4 and t8 on 80% to 95% of the cells. because of the uncommon phenotype, in vitro functional assays were performed that showed decreased mitogenic responses but normal helper activity for b cell immunoglobulin secretion and normal suppressor activity of lectin-induced mitogenesis. morphologic evaluation by both ligh ... | 1985 | 3871160 |
| atopic asthma: t-cell response to corticosteroids. | atopic asthma is associated with diminished cell-mediated immunity and elevated levels of ige, both of which may be caused by imbalances of t-lymphocyte subsets. we analyzed the response of peripheral blood t-cell subsets to two commonly used corticosteroid preparations as a probe of t-cell subset regulation. we administered prednisone (p) 60 mg or 20 mg, beclomethasone dipropionate (bdp) aerosol, 336 micrograms, placebo, or bdp vehicle in a double-blind protocol to 15 atopic asthmatic patients ... | 1985 | 3871188 |
| the isolation and sequence of the gene encoding t8: a molecule defining functional classes of t lymphocytes. | the t cell surface glycoproteins t4 and t8 are thought to mediate efficient cell-cell interactions in the immune system and in this way may be responsible for the appropriate targeting of subpopulations of t cells. we have used gene transfer combined with subtractive hybridization to isolate both cdna and functional genomic clones encoding the t8 protein. the sequence of the cdna reveals that t8 is a transmembrane protein with an n-terminal domain which shares significant homology to immunoglobu ... | 1985 | 3871356 |
| modification of the contractile activity of isolated rat atria by lectin-activated t-lymphocyte subsets. | we had previously shown that human t-lymphocytes (erfc) that had been activated for a short time with phytohemagglutinin (pha) produced positive inotropic and chronotropic effects on spontaneously beating rat atria (sterin-borda, l., et al., naunyn scmiedeberg's arch. pharmacol. 324, 58, 1983). in this study, we first prepared t4-rich (t4) and t8-rich (t8) cells from erfc by selective lysis with okt4 and okt8 monoclonal antibodies and rabbit complement. then, we tested the effect of pha-stimulat ... | 1985 | 3871361 |
| pregnancy-associated growth factor. i. a proliferative agent which expands adult and cord t4 cells in unfractionated cultures. | experiments were designed to examine the effects of pregnancy-associated growth factor (pagf), a substance found in commercial preparations of crude human chorionic gonadotropin (hcg), on unfractionated human cord blood cells (cbc) and adult peripheral blood lymphocytes (pbl) cultured in 5% fetal calf serum (fcs). comparisons of pagf-induced [3h]tdr incorporation in nine pairs of simultaneously cultured cbc and pbl with phytohemagglutinin (pha) and tetanus toxoid (tt) showed that all cbc and pbl ... | 1985 | 3871378 |
| purification and properties of the bacteriophage t4 gene 61 rna priming protein. | the bacteriophage t4 gene 61 protein is required, together with the gene 41 protein and single-stranded dna, for the synthesis of the pentaribonucleotides that are used as primers for the start of each new okazaki dna fragment during t4 dna replication. using this priming activity as an assay, we have purified the 61 protein to essential homogeneity in milligram amounts. the priming activity was identified with the product of t4 gene 61 by using two-dimensional polyacrylamide gel electrophoresis ... | 1985 | 3871437 |
| ontogeny of the t-cell antigen receptor within the thymus. | the expression of t-cell antigen receptors during t-cell ontogeny is an important issue that bears directly on such questions as where t-cell tolerance is acquired, at what stage t cells become susceptible to repertoire selection, and why most thymocytes die within the thymus. the thymus rudiment is colonized during days 11 and 12 of gestation, but it is not until day 19 that significant numbers of functional thymocytes are present. although much is known about the ontogeny of function- and spec ... | 1985 | 3871511 |
| glucocorticoids enhance the in vitro ig synthesis of pokeweed mitogen-stimulated human b cells by inhibiting the suppressive effect of t8+ t cells. | the in vitro effects of three potent glucocorticoids (gc) (dexamethasone, prednisolone, cortisone) on human lymphocyte functions were investigated. in pharmacological concentrations gc strongly suppressed lymphocyte transformation induced by t-cell mitogen (concanavalin a and phytohaemagglutinin). after pokeweed mitogen (pwm) stimulation gc enhanced the number of immunoglobulin (ig)-producing cells without affecting the proliferative response. mineralocorticoid aldosterone showed no effect. addi ... | 1985 | 3871537 |
| the effects of hyperthyroidism on experimental autoimmune thyroiditis in the rat. | the effects of hyperthyroidism on experimental autoimmune thyroiditis were investigated in the rat. rats were given t4 twice daily by sc injection in amounts sufficient to raise circulating hormone levels 10-fold 4 h after administration. thyroiditis was induced by immunization with rat thyroglobulin (tg) in complete freund's adjuvant, and the severity of the disease was assessed by comparison with saline-treated controls. thymic and splenic hypertrophy were found in t4-treated animals, whereas ... | 1985 | 3871692 |
| frequency and surface phenotype of human t lymphocytes producing interleukin 2. analysis by limiting dilution and cell cloning. | in this study we have determined the frequency and distribution of interleukin 2 (il2)-producing cells and their precursors (il2-p) in the two major subsets of human t cells. the two subsets were identified on the basis of their reactivity (or lack thereof) with anti-t4 or anti-t8 monoclonal antibodies. t4+t8- and t4-t8+ cells were first isolated from peripheral blood t cell populations by positive or negative selection using the fluorescence-activated cell sorter, and then analyzed for total il ... | 1985 | 3871697 |
| agar human t cell colony growth promoted by a b + null cell-derived lymphokine distinct from il 2. | human t cell agar colonies can be grown under pha stimulation from either mature t cells or their e rosette-negative (e-), okt3- peripheral blood and bone marrow precursors. colonies comprise a majority of mature e+, okt3+ cells and a minor (5 to 10%) population of immature e-, t3-, t8-, t4-, dr+, t10+, rfb1+ cells, which upon replating in subculture, can generate secondary colonies of okt3+, e+, okt4+, okt8+ cells. secondary colony formation can serve as a test for growth requirement of colony ... | 1985 | 3871813 |
| heterogeneity of human natural killer cells. | the cell surface antigens and specificity of cultures of human natural killer (nk) cells, propagated in the presence of interleukin 2, were analyzed at the single-cell level. with the use of a micropipette isolation procedure, clones were initiated from cells of defined phenotype. the cell surface markers on the resultant clones were not stable and often diverged from those on the cells selected for initiation. clones manifesting nk activity had low expression of t4 and t8 and most had some expr ... | 1985 | 3871873 |
| [hla and myasthenia. subdivision in 3 categories]. | genetic susceptibility (hla types), clinical and pathological findings, amount of acetylcholine receptor antibodies and t lymphocyte subpopulations were studied in 63 patients with myasthenia gravis (mg). the frequency of hla-dr5 was increased among patients (0.50 versus 0.23 in controls, pc less than 0.01, relative risk 3.3) and that of hla-dr3 previously described as associated with mg was slightly increased (0.31 versus 0.20 in controls). the relative frequencies of two t cell subpopulations ... | 1985 | 3871932 |
| human t-cell clones from autoimmune thyroid glands: specific recognition of autologous thyroid cells. | the thyroid glands of patients with autoimmune diseases such as graves' disease and certain forms of goiter contain infiltrating activated t lymphocytes and, unlike cells of normal glands, the epithelial follicular cells strongly express histocompatibility antigens of the hla-dr type. in a study of such autoimmune disorders, the infiltrating t cells from the thyroid glands of two patients with graves' disease were cloned in mitogen-free interleukin-2 (t-cell growth factor). the clones were expan ... | 1985 | 3871967 |
| correlation between immunologic function and clinical subpopulations of patients with the acquired immune deficiency syndrome. | the present study was designed to determine whether there was a significant correlation between the clinical presentation of patients with aids or aids-related illnesses and the degree of their underlying immunologic abnormalities. in 17 patients who presented with opportunistic infections, the mean number of t4 lymphocytes was 34/mm3 and the mean proliferative response to phytohemagglutinin 26,000 cpm; in 12 patients who presented with kaposi's sarcoma alone, the mean number of t4 cells was 231 ... | 1985 | 3872068 |
| a longitudinal evaluation of thyroid function in critically ill surgical patients. | thyroid hormone alterations (known as the "sick-euthyroid syndrome") are common following major surgery, but the time course for appearance and recovery from these alterations has not previously been longitudinally studied in a large group of surgical patients. the authors prospectively studied 59 patients undergoing major surgery (coronary artery bypass grafting, pneumonectomy, or subtotal colectomy). compared with preoperative values, the mean serum t4, t3, free t3, and tsh concentrations decr ... | 1985 | 3872103 |
| in vitro expansion and analysis of cloned cytotoxic t cells derived from patients with chronic t gamma lymphoproliferative disorders. | patients with t gamma lymphocytosis are a heterogeneous group, clearly distinguishable from other patients with chronic t lymphoproliferative disorders and usually without proven malignancy. we have attempted in vitro cloning of lymphocytes from three patients with an expansion of phenotypically and functionally different types of t gamma cells. one had t3+ b73.1+ t4 t8+ okm1+ t gamma cells exerting antibody-dependent cellular cytotoxicity (adcc) and natural killer (nk) cell cytotoxicity; anothe ... | 1985 | 3872139 |
| effect of chemotherapy on t-lymphocyte subsets in b-cell proliferative disorders. | the t-cell subset distribution and the activity markers of 41 patients with multiple myeloma, waldenström's macroglobulinaemia and benign monoclonal gammopathy were repeatedly analysed using monoclonal antibodies (t3, t4, t8, anti-tac, anti dr) and rosetting techniques. in myeloma and macroglobulinaemia the relative and absolute numbers of t4+ cells were diminished while the absolute number of t8+ cells was not decreased. no significant difference between stage i and iii of the myeloma disease w ... | 1985 | 3872145 |
| epidermal growth factor in mouse ocular tissue: effects of thyroxine and exogenous epidermal growth factor. | using a specific and sensitive epidermal growth factor (egf) radioimmunoassay, we identified radioimmunoassayable egf both in developing and adult mouse ocular tissues. in neonatal animals ocular egf concentrations increase during the first 4-9 days and then decline between 9 and 15 days. thyroxine (t4) administration (0.4 micrograms/g body weight/day from day 0) increased local egf concentrations in eye and skin of 7-day-old neonatal mouse pups. however, this treatment did not affect submandibu ... | 1985 | 3872442 |
| cloning, nucleotide sequence, and overexpression of the bacteriophage t4 rega gene. | the bacteriophage t4 rega gene codes for a regulatory protein that controls the expression of a number of t4 early genes, apparently at the level of translation. restriction fragments containing the rega structural gene have been cloned into phage m13, and the nucleotide sequence has been determined. translation of the dna sequence predicted that rega protein contains 122 amino acids, with a mr of 14,620. a dna fragment carrying 85% of the coding sequence of rega has been cloned into the phage l ... | 1985 | 3872458 |
| epidermal growth factor in mouse urine: non-blood origin, and increase by sialoadenectomy and t4 therapy. | to study the origin and regulation of urinary epidermal growth factor (u-egf), we measured egf in adult female mouse plasma, urine and kidney tissue. we also studied the effects of excision of the submandibular salivary gland (smg) and, after smg excision and sham operation, 10-day therapies with t4 (0.4 microgram/g daily), testosterone propionate (tp, 12.5 micrograms/g every 48 h), and smg extract (25 micrograms egf daily). we measured egf with a specific liquid phase radioimmunoassay. the mean ... | 1985 | 3872550 |
| disproportionate lymphoid cell subsets in thalassaemia major: the relative contributions of transfusion and splenectomy. | the relative proportions of t-cell (okt3-positive, okt4-positive and okt8-positive) and b-cell (sig-positive) populations in peripheral blood obtained from 29 chronically transfused patients with beta-thalassaemia major were compared with those of 17 healthy controls. changes attributable to blood transfusion and/or splenectomy are described. the percentage of okt8-positive (t-suppressor) cells found in the thalassaemic patients increased linearly (p less than 0.001) with the number of units tra ... | 1985 | 3872679 |
| phorbol ester-induced differentiation of chronic b lymphocytic leukaemia cells--regulatory impact of autologous and allogeneic accessory cells. | phorbol ester (tpa) induction of chronic lymphocytic leukaemia (cll) cells can be used as a model system for the study of human b cell differentiation. we have analysed the role of accessory cells and the correlation to target cell surface ig phenotype in tpa-induced morphological and functional differentiation of 12 cll populations. a more than three-fold increase in secreted igm was seen in 10 of 12 cases, with the strongest responses in patients having monoclonal serum igm. cll populations ne ... | 1985 | 3872753 |
| enhancement of mitogen response and surface marker analysis of lymphocytes from young and old donors after preliminary incubation in vitro. | we have examined the detailed kinetics of pha induced proliferation of freshly isolated mononuclear cells from young and old human donors. our studies confirm that donors over the age of 60 years may have a decreased number of pha responsive cells and that these cells have a significantly diminished rate of entry into the first cell cycle. age of the donor does not affect the time at which significant numbers of cells appear in first s-phase, the duration of first s-phase, the doubling time in e ... | 1985 | 3872977 |
| implantation site in complete molar pregnancy: a study of immunologically competent cells with monoclonal antibodies. | the nature and intensity of inflammatory cellular infiltrate in the implantation sites of 10 complete molar pregnancies were evaluated by immunohistochemical staining of frozen tissue sections with monoclonal antibodies. as compared to the implantation site in normal pregnancy, there was an increase in the number of infiltrating inflammatory cells in the molar implantation site. most of the inflammatory cells were t cells with predominance of t4+ (leu-3a+) cells over t8+ cells. inflammatory cell ... | 1985 | 3873176 |
| lymphocyte subpopulations in graves' ophthalmopathy. | we assessed the percentages of t-lymphocyte subsets, b lymphocytes, monocytes, and granulocytes in the mononuclear cell preparations of euthyroid patients with minimal and severe graves' ophthalmopathy. patients with active werner class 4-5 and class 6 ophthalmopathy had statistically significant elevations of suppressor/cytotoxic t8+ lymphocytes. during successful corticosteroid therapy, the number of t8+ lymphocytes returned to the normal range. mirroring these results, the t4/t8 ratio was ini ... | 1985 | 3873236 |
| hydrodynamic studies of a dna-protein complex. dimensions of the complex of single-stranded 145 base dna with gene 32 protein of phage t4 deduced from quasi-elastic light scattering. | the translational diffusion coefficient of the saturated complex of single-stranded 145 base dna and the helix-destabilizing protein of phage t4, gp32, can be measured at equilibrium by means of quasi-elastic light scattering. if the complex is considered as a rigid rod one can estimate its dimensions by combining the translational diffusion coefficient with earlier data on rotational diffusion. it was found that the average base-base distance of the 145 base dna in the complex is between 4.3 an ... | 1985 | 3873355 |
| somatic gene mutations in vivo as indicated by the 6-thioguanine-resistant t-lymphocytes in human blood. | the clonal and the autoradiographic assays for 6-thioguanine-resistant (tgr) t-lymphocytes (t-lys) in human blood are reviewed. studies of tgr colonies recovered from clonal assays show that the mutant t-lys (i) are either helper (t4) or suppressor (t8) cells, (ii) possess stable tgr phenotypes, (iii) are deficient in hypoxanthine guanine phosphoribosyltransferase (hprt), and (iv) have structural alterations in the hprt gene. tgr t-ly mutant frequencies (mfs) determined by clonal assays are of t ... | 1985 | 3873612 |