Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| rapid detection and identification of infectious pathogens based on high-throughput sequencing. | the dilemma of pathogens identification in patients with unidentified clinical symptoms such as fever of unknown origin exists, which not only poses a challenge to both the diagnostic and therapeutic process by itself, but also to expert physicians. | 2015 | 25836606 |
| g-quadruplexes in pathogens: a common route to virulence control? | dna can form several secondary structures besides the classic double helix: one that has received much attention in recent years is the g-quadruplex (g4). this is a stable four-stranded structure formed by the stacking of quartets of guanine bases. recent work has convincingly shown that g4s can form in vivo as well as in vitro and can affect both replication and transcription of dna. they also play important roles at g-rich telomeres. now, a spate of exciting reports has begun to reveal roles f ... | 2015 | 25654363 |
| epidemiology of meningitis in an hiv-infected ugandan cohort. | there is limited understanding of the epidemiology of meningitis among human immunodeficiency virus (hiv)-infected populations in sub-saharan africa. we conducted a prospective cohort study of hiv-infected adults with suspected meningitis in uganda, to comprehensively evaluate the etiologies of meningitis. intensive cerebrospiral fluid (csf) testing was performed to evaluate for bacterial, viral, fungal, and mycobacterial etiologies, including neurosyphilis,16s ribosomal dna (rdna) polymerase ch ... | 2015 | 25385864 |
| dysbiotic infection in the stomach. | microbiota in human alimentary tract plays important roles for homeostatic maintenance of the body. compositional difference of gut microbiota is tightly associated with susceptibility of many diseases, including inflammatory diseases, obesity, diabetes mellitus, cancer, and atherosclerosis. "dysbiosis" refers to a state of imbalance among the colonies of microorganisms within the body, which brings abnormal increase of specific minor components and decrease in the normally dominant species. sin ... | 2015 | 26523109 |
| immunoproteomic profiling of antiviral antibodies in new-onset type 1 diabetes using protein arrays. | the rapid rise in the incidence of type 1 diabetes (t1d) suggests the involvement of environmental factors including viral infections. we evaluated the association between viral infections and t1d by profiling antiviral antibodies using a high-throughput immunoproteomics approach in patients with new-onset t1d. we constructed a viral protein array comprising the complete proteomes of seven viruses associated with t1d and open reading frames from other common viruses. antibody responses to 646 vi ... | 2015 | 26450993 |
| association between herpesviruses and chronic periodontitis: a meta-analysis based on case-control studies. | numerous studies have investigated the associations between herpesviruses and chronic periodontitis; however, the results remain controversial. to derive a more precise estimation, a meta-analysis on all available studies was performed to identify the association between herpesviruses and chronic periodontitis. | 2015 | 26666412 |
| multicenter study of combination dep regimen as a salvage therapy for adult refractory hemophagocytic lymphohistiocytosis. | hemophagocytic lymphohistiocytosis (hlh) is a refractory immune disorder with a significant risk of death. although standard therapy has dramatically improved survival in hlh patients, approximately 30%, especially adults, show no response to current treatment strategies. this prospective study aimed to investigate the efficacy of liposomal doxorubicin treatment combined with etoposide and methylprednisolone (doxorubicin-etoposide-methylprednisolone; dep) as a salvage therapy for adult refractor ... | 2015 | 26289641 |
| therapeutic antiviral t cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells. | several viruses can infect the mammalian nervous system and induce neurological dysfunction. adoptive immunotherapy is an approach that involves administration of antiviral t cells and has shown promise in clinical studies for the treatment of peripheral virus infections in humans such as cytomegalovirus (cmv), epstein-barr virus (ebv), and adenovirus, among others. in contrast, clearance of neurotropic infections is particularly challenging because the central nervous system (cns) is relatively ... | 2015 | 26122661 |
| xenosurveillance: a novel mosquito-based approach for examining the human-pathogen landscape. | globally, regions at the highest risk for emerging infectious diseases are often the ones with the fewest resources. as a result, implementing sustainable infectious disease surveillance systems in these regions is challenging. the cost of these programs and difficulties associated with collecting, storing and transporting relevant samples have hindered them in the regions where they are most needed. therefore, we tested the sensitivity and feasibility of a novel surveillance technique called xe ... | 2015 | 25775236 |
| the role of latently infected b cells in cns autoimmunity. | the onset of multiple sclerosis (ms) is caused by both genetic and environmental factors. among the environmental factors, it is believed that previous infection with epstein-barr virus (ebv) may contribute in the development of ms. ebv has been associated with other autoimmune diseases, such as systemic lupus erythematous, and cancers like burkitt's lymphoma. ebv establishes a life-long latency in b cells with occasional reactivation of the virus throughout the individual's life. the role playe ... | 2015 | 26579121 |
| basophil activation by mosquito extracts in patients with hypersensitivity to mosquito bites. | hypersensitivity to mosquito bites (hmb) is a cutaneous disorder belonging to the group of epstein-barr virus (ebv)-associated t/natural killer (nk)-cell lymphoproliferative diseases, and is primarily mediated by ebv-infected nk cells. it is characterized by intense local skin reactions accompanied by general symptoms after mosquito bites, and infiltration of ebv-infected nk cells into the bite sites. however, the mechanisms underlying these reactions have not been fully examined. we recently de ... | 2015 | 25990049 |
| java project on periodontal diseases: effect of vitamin c/calcium threonate/citrus flavonoids supplementation on periodontal pathogens, crp and hba1c. | to assess in a periodontally diseased rural population deprived from regular dental care and having poor dietary conditions, the effect of vitamin c/calcium threonate/citrus flavonoids (vitc/ca/fl) supplementation on subgingival microbiota and plasma levels of vitamin c, hba1c and hscrp. | 2015 | 26549279 |
| revelation of viral - bacterial interrelationship in aggressive periodontitis via polymerase chain reaction: a microbiological study. | periodontal disease is one of the most common and complex disease affecting mankind. being multifactorial in etiology it encompasses a variety of infectious entities with various unique microbial constellations and immune responses. a bacteriologic cause alone seems insufficient in explaining several clinical features of the periodontal disease. recent studies suggest that periodontal herpes viruses comprise an important source of triggering periodontal tissue destruction. the following study ai ... | 2015 | 26435627 |
| periodontopathogen and epstein-barr virus-associated periapical periodontitis may be the source of retrograde infectious peri-implantitis. | herpesviral-bacterial synergism may play a role in periodontitis and peri-implantitis etiopathogenesis. periapical periodontitis (pp) lesions can predict future apical peri-implantitis complications. | 2015 | 23675952 |
| protection from varicella zoster in solid organ transplant recipients carrying killer cell immunoglobulin-like receptor b haplotypes. | natural killer cell function is regulated by inhibitory and activating killer cell immunoglobulin-like receptors (kir). previous studies have documented associations of kir genotype with the risk of cytomegalovirus (cmv) replication after solid organ transplantation. | 2015 | 26050016 |
| quantitative nucleic acid amplification methods for viral infections. | over the past 2 decades there have been substantial improvements in the methods used to quantify viral nucleic acid in body fluids and in our understanding of how to use viral load measurements in the diagnosis and management of patients with a number of viral infections. these methods are now integrated into a wide range of diagnostic and treatment guidelines and commonly deployed in a variety of clinical settings. | 2015 | 25403817 |
| the impact of donor viral replication at transplant on recipient infections posttransplant: a prospective study. | organ donors are often implicated as the source of posttransplant recipient infection. we prospectively studied kidney and liver donor-recipient pairs to determine if donor viral replication of cytomegalovirus (cmv), epstein-barr virus (ebv), and bk polyomavirus (bkv) at transplant was a risk factor for posttransplant recipient infection and disease. | 2015 | 25148381 |
| differences in antigen-specific cd4+ responses to opportunistic infections in hiv infection. | hiv-infected individuals with severe immunodeficiency are at risk of opportunistic infection (oi). tuberculosis (tb) may occur without substantial immune suppression suggesting an early and sustained adverse impact of hiv on mycobacterium tuberculosis (mtb)-specific cell mediated immunity (cmi). this prospective observational cohort study aimed to observe differences in oi-specific and mtb-specific cmi that might underlie this. using polychromatic flow cytometry, we compared cd4+ responses to mt ... | 2015 | 26417433 |
| the etiology of juvenile idiopathic arthritis. | over the years, the commonly used term to describe juvenile idiopathic arthritis (jia) has changed. by definition, jia includes all types of arthritis with no apparent cause, lasting more than 6 weeks, in patients aged less than 16 years at onset. jia pathogenesis is still poorly understood: the interaction between environmental factors and multiple genes has been proposed as the most relevant working mechanism to the development of jia. the concept that various microbes that colonize or infect ... | 2015 | 25384710 |
| multiple sclerosis between genetics and infections: human endogenous retroviruses in monocytes and macrophages. | the etiology of multiple sclerosis (ms) is still unknown, but there is strong evidence that genetic predisposition associated with environmental factors can trigger the disease. an estimated 30 million years ago, exogenous retroviruses are thought to have integrated themselves into human germ line cells, becoming part of human dna and being transmitted over generations. usually such human endogenous retroviruses (hervs) are silenced or expressed at low levels, but in some pathological conditions ... | 2015 | 26734011 |
| the association between infectious burden and parkinson's disease: a case-control study. | the etiology of parkinson's disease (pd) remains unclear. the aim of this study was to examine the association between common pathogenic infections and pd. | 2015 | 26037459 |
| upregulation of caveolin-1 correlate with akt expression and poor prognosis in npc patients. | the aim of this study is to investigate the role of caveolin-1 (cav-1) in nasopharyngeal carcinoma (npc) progression and correlated with clinical outcomes. | 2015 | 25877996 |
| risk factors associated with the onset of relapsing-remitting and primary progressive multiple sclerosis: a systematic review. | multiple sclerosis (ms) is a chronic central nervous system disease with a highly heterogeneous course. the aetiology of ms is not well understood but is likely a combination of both genetic and environmental factors. approximately 85% of patients present with relapsing-remitting ms (rrms), while 10-15% present with primary progressive ms (ppms). ppms is associated with an older onset age, a different sex ratio, and a considerably more rapid disease progression relative to rrms. we systematicall ... | 2015 | 25802867 |
| the aetiology of pharyngotonsillitis in adolescents and adults - fusobacterium necrophorum is commonly found. | sore throat is common in primary healthcare. aetiological studies have focused on the presence of a limited number of pathogens. the aim of the present study was to investigate the presence of a wide range of bacteria and viruses, including fusobacterium necrophorum, in patients with pharyngotonsillitis and in asymptomatic controls. a prospective case control study was performed in primary healthcare in kronoberg county, sweden. patients (n=220) aged 15 to 45 years with a suspected acute pharyng ... | 2015 | 25658556 |
| viral exanthems. | determining the viral cause of a rash presents significant diagnostic challenges. we review contemporary literature on viral exanthems and suggest a structured approach to aid diagnosis. | 2015 | 25706914 |
| the first report of a previously undescribed ebv-negative nk-cell lymphoma of the gi tract presenting as chronic diarrhoea with eosinophilia. | a 74-year-old man presented with a 2-month history of watery diarrhoea. his complete blood count showed lymphopaenia and marked eosinophilia. investigations for common infectious causes including clostridium difficile toxin, stool culture, ova and parasites were negative. endoscopy revealed extensive colitis and a ct of the abdomen identified numerous large abdominal lymph nodes suspicious for lymphoma. multiple tissue samples were obtained; colon, mesenteric lymph node and bone marrow biopsy, a ... | 2015 | 26611482 |
| evaluation of a multiplex pcr assay for detection of cytomegalovirus in stool samples from patients with ulcerative colitis. | to evaluate a multiplex pcr assay for the detection of bacterial and viral enteropathogens in stool samples from patients with ulcerative colitis (uc). | 2015 | 26640344 |
| detection of epstein-barr virus dna in langerhans cell histiocytosis. | langerhans cell histiocytosis (lch) is a rare histiocytic proliferation of unknown etiology. it is characterized by granuloma-like proliferation of langerhans-type dendritic cells and mainly affects young children. although multiple investigators have suggested the possible role of viruses, such as epstein-barr virus (ebv), human herpesvirus-6 (hhv-6), herpes simplex virus (hsv) types 1 and 2, and cytomegalovirus (cmv) in the pathogenesis of lch, it remains, however, debated. | 2015 | 26870310 |
| a hospital based pilot study on epstein-barr virus in suspected infectious mononucleosis pediatric patients in india. | infectious mononucleosis (im) caused by the epstein-barr virus (ebv) is commonly diagnosed by detection of antibodies in the patient's sera. differentiation of acute from chronic and differential diagnosis of ebv-induced im from im-like syndrome caused by human cytomegalovirus (cmv) is important. the objective of this study was to standardize and use polymerase chain reaction (pcr) for diagnosis of ebv and evaluate it against enzyme-linked immunosorbent assay (elisa). | 2015 | 26517489 |
| association of herpes viruses with mild, moderate and severe chronic periodontitis. | periodontal disease is an inflammatory condition of the supporting tissues of the teeth. it is a multi-factorial and multi-etiological infectious disease process. recent evidences shows that human herpes viruses could be putative pathogens. the aim of the present study was to evaluate the association of herpes viruses especially herpes simplex viruses (hsv-1 and 2), epstein-barr virus (ebv) and human cytomegalovirus (hcmv) in patients with chronic periodontitis. | 2015 | 26393126 |
| role of non-hepatotropic viruses in acute sporadic viral hepatitis and acute-on-chronic liver failure in adults. | acute viral hepatitis (avh) is mostly caused by hepatitis e (hev) and hepatitis a (hav) viruses in india. this study was undertaken to find out the incidence of various hepatotropic and non-hepatotropic viruses in avh and acute-on-chronic liver failure (aclf) patients. | 2015 | 26589230 |
| coprevalence of epstein-barr virus, cytomegalovirus, and herpes simplex virus type-1 antibodies among united states children and factors associated with their acquisition. | epstein-barr virus (ebv), cytomegalovirus (cmv), and herpes simplex virus type-1 (hsv-1) infections are common worldwide, but age-specific prevalence of primary infection varies by race or ethnicity and geographical location. comparing demographic groups could identify factors influencing the rate of acquisition, age-specific antibody prevalence is relevant for determining when to administer prophylactic vaccines, and coprevalence suggests similar risk factors. | 2015 | 26582871 |
| study of the frequency of herpesvirus infections among patients suspected aseptic meningitis in the west of iran. | aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). | 2015 | 26568804 |
| cytomegalovirus reactivation and its clinical impact in patients with solid tumors. | cytomegalovirus reactivation can be life threatening. however, little evidence on its incidence in solid cancers is available. therefore our single center cytomegalovirus polymerase chain reaction database with altogether 890 cmv positive blood serum samples of mainly hematological and oncological patients was retrospectively analyzed to examine the occurrence of cytomegalovirus reactivation in patients with solid tumors, resulting in 107 patients tested positive for cytomegalovirus reactivation ... | 2015 | 26635891 |
| a diverse repertoire of cd4 t cells targets the immediate-early 1 protein of human cytomegalovirus. | t-cell responses to the immediate-early 1 (ie-1) protein of human cytomegalovirus (hcmv) are associated with protection from viral disease. thus, ie-1 is a promising target for immunotherapy. cd8 t-cell responses to ie-1 are generally strong. in contrast, cd4 t-cell responses to ie-1 were described to be comparatively infrequent or undetectable in hcmv carriers, and information on their target epitopes and their function has been limited. to analyze the repertoire of ie-1-specific cd4 t cells, w ... | 2015 | 26635812 |
| visceral leishmaniasis in a patient with systemic lupus erythematosus. | visceral leishmaniasis is an infection with an insidious and disabling course caused by parasites of the genus leishmania. in europe, it is mostly associated with hiv infection. systemic lupus erythematosus and its treatment are associated with increased risk of infection, neoplastic and concomitant autoimmune disorders. the association of these diseases may go unnoticed. a 60 year-old caucasian woman with lupus presented with a one-year history of fever, malaise, weakness and weight loss. the h ... | 2015 | 26793472 |
| the prevalence of infectious agents in patients with systemic sclerosis. | systemic sclerosis (ssc) is an autoimmune disease characterized by microvascular injury, excessive extracellular matrix deposition, and fibrosis in the skin and internal organs. bacterial and viral infectious agents have been suspected to be contributing factors in the development and progression of the pathologic features of ssc. | 2015 | 26775370 |
| [treatment of gaucher disease with allogeneic hematopoietic stem cell transplantation: report of three cases and review of literatures]. | to explore the efficacy of unrelated umbilical cord blood transplantation (ucbt) in the treatment of gaucher disease. | 2015 | 26758318 |
| assessment of igg antibodies against hsv1, hsv2, cmv and ebv in patients with pemphigus vulgaris versus healthy people. | regarding the implication of viruses particularly herpes in pemphigus vulgaris, we sought to assess and compare the level of immunoglobulin g (igg) antibodies against herpes simplex virus types 1 and 2 (hsv1 and hsv2), cytomegalovirus (cmv) and epstein-barr virus (ebv) in patients with pemphigus vulgaris and healthy people. | 2015 | 27507994 |
| lack of association between herpesvirus detection in saliva and gingivitis in hiv‑infected children. | the aims of this study were to compare the detection of human herpesviruses (hhvs) in the saliva of hiv-infected and healthy control children, and to evaluate associations between viral infection and gingivitis and immunodeficiency. saliva samples were collected from 48 hiv-infected and 48 healthy control children. clinical and laboratory data were collected during dental visits and from medical records. a trained dentist determined gingival indices and extension of gingivitis. saliva samples we ... | 2015 | 26200962 |
| ebv, hsv, cmv and hpv in laryngeal and oropharyngeal carcinoma in polish patients. | the role of viruses in the etiology of oral cancer has been proposed in many studies. the aim of the present study was to analyze the prevalence of epstein-barr virus, human herpes virus type 1, cytomegalovirus and human papilloma virus among patients with oral squamous cell carcinoma in a polish population. we investigated fresh-frozen tumor tissue fragments obtained from 80 patients with oscc using the polymerase chain reaction assay. hpv was detected in 32.5% (22.5% were hpv 16), more often i ... | 2015 | 25750324 |
| antibodies to early ebv, cmv, and hhv6 antigens in systemic lupus erythematosus patients. | we investigated the antibody levels against early antigens of epstein-barr virus (ebv), cytomegalovirus (cmv), and human herpesvirus 6 (hhv6) in systemic lupus erythematosus (sle) patients and healthy controls, and further correlated these antibodies to haematology/biochemistry, serology, and disease activity measures. | 2015 | 25562120 |
| the association between antibodies to neurotropic pathogens and schizophrenia: a case-control study. | exposure to neurotropic pathogens has been proposed as an environmental risk factor for schizophrenia and can be evaluated by measuring pathogen-specific immunoglobulin g (igg). seroprevalence of pathogen-specific igg reflects prior exposure, whereas igg levels are associated with reactivity or reinfection. several studies have examined these parameters in schizophrenia. however, results still remain inconclusive, as several previous studies did not correct for important confounding factors. | 2015 | 27336045 |
| oral ulceration associated with concurrent herpes simplex virus, cytomegalovirus, and epstein-barr virus infection in an immunocompromised patient. | in immunocompromised patients, oral ulcerations are common and have a wide spectrum of causes, including herpesvirus infection. we report on a case in which an oral ulcer was simultaneously infected by herpes simplex (hsv), cytomegalovirus (cmv), and epstein-barr virus (ebv) in a kidney-pancreas transplant recipient. a 46-year-old woman presented with a clinically nonspecific dorsal tongue ulcer of 3 months duration. histopathologic evaluation indicated keratinocytes exhibiting herpetic viral cy ... | 2015 | 25544404 |
| cytomegalovirus, and possibly epstein-barr virus, shedding in breast milk is associated with hiv-1 transmission by breastfeeding. | postnatal hiv-1 mother-to-child transmission (mtct) occurs in spite of antiretroviral therapy. co-infections in breast milk with cytomegalovirus (cmv) and epstein-barr virus (ebv) are associated with increased hiv-1 shedding in this compartment. we investigated cmv levels and ebv detection in breast milk as potential risk factors for mtct of hiv-1 via breastfeeding. | 2015 | 25535751 |
| valganciclovir administration to kidney donors to reduce the burden of cytomegalovirus and epstein-barr virus transmission during transplantation. | cytomegalovirus (cmv) and epstein-barr virus (ebv) infections are a significant cause of morbidity and mortality in transplant recipients and are often transmitted from the donor organ. | 2015 | 25489844 |
| viruses and disease: emerging concepts for prevention, diagnosis and treatment. | viruses cause a wide range of human diseases, ranging from acute self-resolving conditions to acute fatal diseases. effects that arise long after the primary infection can also increase the propensity for chronic conditions or lead to the development of cancer. recent advances in the fields of virology and pathology have been fundamental in improving our understanding of viral pathogenesis, in providing improved vaccination strategies and in developing newer, more effective treatments for patien ... | 2015 | 25366544 |
| genotypic detection of epstein barr virus from clinically suspected viral retinitis patients in a tertiary eye care centre, india. | to evaluate the diagnostic value of pcr on aqueous humour for detection and genotyping of epstein bar virus in patients with viral retinitis. | 2015 | 25325148 |
| expression of cyclin-dependent kinase inhibitor 2a 16, tumour protein 53 and epidermal growth factor receptor in salivary gland carcinomas is not associated with oncogenic virus infection. | it is known that human papillomavirus (hpv) infection can cause squamous cell neoplasms at several sites, such as cervix uteri carcinoma and oral squamous carcinoma. there is little information on the expression of hpv and its predictive markers in tumours of the major and minor salivary glands of the head and neck. we therefore assessed oral salivary gland neoplasms to identify associations between hpv and infection-related epidermal growth factor receptor (egfr), cyclin-dependent kinase inhibi ... | 2015 | 25012870 |
| lymphoma-like syndrome: 4 case reports about atypical presentation of primary cytomegalovirus infection in immunocompetent children. | in immunocompetent persons, primary cytomegalovirus (cmv) infection is self-limited infection. lymphoma-like syndromes have been sometimes described in adults but have not been described for children.lymphoma-like syndromes (protracted fever, alteration of the general status, and clinical lymphoproliferative syndrome) were retrospectively recorded in children attending our hospital from 1999 to 2008 for primary cmv infection. patients with immunodeficiency, coinfection (epstein-barr virus, toxop ... | 2015 | 26131836 |
| animal herpesviruses and their zoonotic potential for cross-species infection. | herpesviruses of humans and animals cause severe diseases that influence not only the health and epidemiological status but are also economically important in the context of food production. the members of herpesviridae are host specific agents that also share many properties that potentially make them capable of crossing the species barriers. the objective of presented review paper was to summarize the relationship between herpesviruses of animals and humans and their zoonotic potential. in hum ... | 2015 | 26094506 |
| seroprevalence of epstein-barr virus, cytomegalovirus, and polyomaviruses in children with inflammatory bowel disease. | young age and thiopurine therapy are risk factors for lymphoproliferative disease among patients with inflammatory bowel disease (ibd). | 2015 | 26091801 |
| the seroprevalence and salivary shedding of herpesviruses in behçet's syndrome and recurrent aphthous stomatitis. | behçet's syndrome (bs) is one of the multisystemic diseases that presents with oral ulceration and several other systemic manifestations including genital ulceration, folliculitis, erythema nodosum-like lesions, uveitis, and arthropathy. ocular manifestation, central nervous system involvement, and gastrointestinal manifestation account for most of the complications of this disease, whereas orogenital ulceration and dermatological involvement affects the quality of life. the cause of the disease ... | 2015 | 26051327 |
| broad spectrum antiviral t cells for viral complications after hematopoietic stem cell transplantation. | major complications of hematopoietic stem cell transplantation (hsct) or solid organ transplantation (sot), such as graft rejection and graft-versus-host-disease (gvhd), are countered by suppressing the host immune system via chemotherapy and radiation, immunosuppressive drugs, or conditioning regimens such as in vivo or in vitro t-cell depletion. while immunocompromised, the patient is rendered susceptible to a number of viral infections and reactivations mainly caused by endogenous herpes viru ... | 2015 | 26046087 |
| quantification of total and 2-ltr (long terminal repeat) hiv dna, hiv rna and herpesvirus dna in pbmcs. | almost all individuals infected with human immunodeficiency virus (hiv) are also infected with cytomegalovirus (cmv) and epstein barr virus (ebv). the aims of our studies have included characterizing and measuring the latent hiv reservoir and understanding the association between asymptomatic replication of cmv (and other herpesvirus, including ebv) and this hiv reservoir (gianella et al., 2014). this protocol was designed to simultaneously co-extract dna and rna from the same peripheral blood m ... | 2015 | 27478862 |
| [acute herpesviridae hepatitis during pregnancy: a review]. | viral hepatitis are well defined during pregnancy, including hepatitis a, b, c, d or e. in contrast, viral hepatitis called non-alphabetic, like viruses herpesviridae family hepatitis [cytomegalovirus (cmv), varicella-zoster virus (vzv), epstein-barr virus (ebv) or herpes simplex virus (hsv)] are rarely described. the aim of this article is to make a focus on the care of these viral herpesviridae hepatitis during pregnancy. herpes hepatitis is more common during pregnancy, with a neonatal risk a ... | 2015 | 26033557 |
| prevalence of herpesvirus and correlation with clinical parameters in indian subjects with chronic periodontitis. | the identification of new uncultured species and viruses supports the possibility of combination of the herpes-virus-bacterial periodontal infection for periodontitis. the paucity of data and studies with larger sample size in indian subjects provides an unclear picture of the presence of the herpesvirus in this population. | 2015 | 26718301 |
| addition of varicella zoster virus-specific t cells to cytomegalovirus, epstein-barr virus and adenovirus tri-specific t cells as adoptive immunotherapy in patients undergoing allogeneic hematopoietic stem cell transplantation. | virus-specific t-cell immunotherapy is emerging as a promising management strategy for virus infections in patients after hematopoietic stem cell transplant (hsct). here we present outcomes of 10 adult patients who received multi-virus-specific t cells prophylactically after hsct. | 2015 | 26349000 |
| squash smear cytology of langerhans cell histiocytosis. | squash smear cytology of langerhans cell histiocytosis (lch) has rarely been reported. we described squash cytological findings of cranial lch. additionally, based on recent data that suggests an association of lch with either viral infection or genetic alteration, we investigated the presence of several viruses or mutation of tp53 and braf in lch tissue samples. intraoperative squash smears of a small tissue fragment excised from the lesion demonstrated a mixed population of eosinophils, neutro ... | 2015 | 26339366 |
| performance of a new rapid test for detecting anti-hepatitis e virus immunoglobulin m in immunocompetent and immunocompromised patients. | hepatitis e virus (hev) infections are widespread in both developing and developed countries. it is, therefore, important to assess the performance of systems for rapidly detecting anti-hev immunoglobulin m antibodies in human sera. | 2015 | 26305830 |
| false positivity of monospot test in an immunocompetent elderly woman with acute cytomegalovirus infection. | a 75-year-old woman presented with altered mental status, septic picture, and influenza-like symptoms. initial investigations revealed atypical lymphocytosis, thrombocytopenia, elevated liver enzymes, and a positive monospot test result. further investigation showed the epstein-barr virus viral capsid antibody igm/igg and epstein-barr virus dna by polymerase chain reaction to be negative; however, interestingly her cytomegalovirus (cmv) igm and igg were positive, suggesting that her mononucleosi ... | 2015 | 26275628 |
| early transduction produces highly functional chimeric antigen receptor-modified virus-specific t-cells with central memory markers: a production assistant for cell therapy (pact) translational application. | virus-specific t-cells (vsts) proliferate exponentially after adoptive transfer into hematopoietic stem cell transplant (hsct) recipients, eliminate virus infections, then persist and provide long-term protection from viral disease. if vsts behaved similarly when modified with tumor-specific chimeric antigen receptors (cars), they should have potent anti-tumor activity. this theory was evaluated by cruz et al. in a previous clinical trial with cd19.car-modified vsts, but there was little apparen ... | 2015 | 25734008 |
| human herpes virus reactivations and dynamic cytokine profiles in patients with cutaneous adverse drug reactions --a prospective comparative study. | sequential human herpes virus (hhv) reactivation is well known in drug reaction with eosinophilia and systemic symptom (dress), but such a phenomenon has seldom studied in other types of cutaneous adverse drug reactions (cadrs). moreover, the association between viral reactivations and cytokine or chemokine changes is largely unknown. we aimed to evaluate the viral reactivation rates of hhv-6, hhv-7, epstein-barr virus (ebv), and cytomegalovirus (cmv) in different cadrs and their impacts on clin ... | 2015 | 25727950 |
| cytomegalovirus and epstein-barr virus in breast cancer. | findings of polymerase chain reaction (pcr) studies of cytomegalovirus (cmv) and epstein-barr virus (ebv) and breast cancer vary, making it difficult to determine whether either, both, or neither virus is causally associated with breast cancer. we investigated cmv and ebv in paired samples of breast cancer and normal breast tissue from 70 women using quantitative pcr. a serum sample from each woman was tested for cmv and ebv igg. to place our results in context, we reviewed the existing literatu ... | 2015 | 25723522 |
| a new vaccine escape mutant of hepatitis b virus causes occult infection. | there is growing public concern regarding assay sensitivity to hbsag mutants in clinical diagnosis and vaccine escape. the aim of this study is to introduce a new hbsag mutant strain. the serum samples were those of patient x at the age of 3 months and 3 years respectively, and of her mother immediately before parturition, which were used to amplify the hbsag-coding dna fragments by pcr. the hbsag dna sequences were translated into their corresponding amino acid sequences and then aligned in pub ... | 2015 | 25692622 |
| k562-derived whole-cell vaccine enhances antitumor responses of car-redirected virus-specific cytotoxic t lymphocytes in vivo. | adoptive transfer of epstein-barr virus (ebv)-specific and cytomegalovirus (cmv)-specific cytotoxic t cells (ctl) genetically modified to express a chimeric antigen receptor (car) induces objective tumor responses in clinical trials. in vivo expansion and persistence of these cells are crucial to achieve sustained clinical responses. we aimed to develop an off-the-shelf whole-cell vaccine to boost car-redirected virus-specific ctls in vivo after adoptive transfer. as proof of principle, we valid ... | 2015 | 25691731 |
| development of a time-resolved fluorescence immunoassay for epstein-barr virus zta iga antibodies in human serum. | the epstein-barr virus (ebv) transactivator protein (zebra) is an immediate-early protein that plays an important role in the switch from latency to productive cycle in ebv virus. zebra is an important marker of ebv reactivation. in order to diagnose ebv infection status correctly and timely, a novel immunoassay was developed based on an indirect time-resolved fluoroimmunoassay (trfia) for zta iga, which used recombinant zta antigen as solid-phase antigen and eu(3+)-labeled mouse antihuman iga a ... | 2015 | 25651045 |
| impact of epstein barr virus-related complications after high-risk allo-sct in the era of pre-emptive rituximab. | we monitored 133 high-risk allo-sct recipients for 6 months after transplant for ebv reactivation by quantitative real-time pcr. rituximab was given as pre-emptive therapy for viremia >1000 copies/ml. the 1-year cumulative incidence of ebv reactivation was 29.4% (95% confidence interval (ci): 18-40) in patients monitored due to initial high-risk characteristics (n=93) and 31.8% (95% ci: 19.7-44) in those followed because of the development of refractory gvhd (n=40). overall response rate to ritu ... | 2015 | 25581404 |
| 6-year-old boy with recurrent sinopulmonary infections and lymphadenopathy. | 2015 | 25956317 | |
| effects of tofacitinib on lymphocyte sub-populations, cmv and ebv viral load in patients with plaque psoriasis. | plaque psoriasis is a debilitating skin condition that affects approximately 2% of the adult population and for which there is currently no cure. tofacitinib is an oral janus kinase inhibitor that is being investigated for psoriasis. | 2015 | 25951857 |
| large-scale multiplex polymerase chain reaction assay for diagnosis of viral reactivations after allogeneic hematopoietic stem cell transplantation. | viral reactivations following hematopoietic stem cell transplantation are thought to result from the breakdown of both cell-mediated and humoral immunity. as a result, many viruses could be reactivated individually or simultaneously. using a multiplex polymerase chain reaction (pcr), we prospectively examined many kinds of viral dnas at a time in 105 patients who underwent allogeneic hematopoietic stem cell transplantation. in total, 591 whole blood samples were collected weekly from pre- to 42 ... | 2015 | 25946433 |
| donor cd4 t cell diversity determines virus reactivation in patients after hla-matched allogeneic stem cell transplantation. | delayed reconstitution of the t cell compartment in recipients of allogeneic stem cell grafts is associated with an increase of reactivation of latent viruses. thereby, the transplanted t cell repertoire appears to be one of the factors that affect t cell reconstitution. therefore, we studied the t cell receptor beta (tcrβ) gene rearrangements of flow cytometry-sorted cd4(+) and cd8(+) t cells from the peripheral blood of 23 allogeneic donors before g-csf administration and on the day of apheres ... | 2015 | 25873100 |
| selection of adenovirus-specific and epstein-barr virus-specific t cells with major histocompatibility class i streptamers under good manufacturing practice (gmp)-compliant conditions. | despite antiviral drug therapies, human adenovirus (hadv), cytomegalovirus (cmv) and epstein-barr virus (ebv) infections still contribute substantially to transplant-related death of patients after hematopoietic stem cell transplantation. earlier clinical studies demonstrated successful adoptive transfer of magnetically selected cmv-specific t cells via removable, and thus good manufacturing practice-compliant, major histocompatibility class i streptamers. thus, the primary focus of the present ... | 2015 | 25866178 |
| anti-cmv-igg positivity of donors is beneficial for allohsct recipients with respect to the better short-term immunological recovery and high level of cd4+cd25high lymphocytes. | hematopoietic stem cell transplantation from anti-cytomegalovirus immunoglobulin g (anti-cmv-igg) positive donors facilitated immunological recovery post-transplant, which may indicate that chronic cmv infection has an effect on the immune system. this can be seen in the recipients after reconstitution with donor lymphocytes. we evaluated the composition of lymphocytes at hematologic recovery in 99 patients with hematologic malignancies post hematopoietic stem cell transplantation (hsct). anti-c ... | 2015 | 25807050 |
| viral-induced cd28 loss evokes costimulation independent alloimmunity. | belatacept, a b7-specific fusion protein, blocks cd28-b7 costimulation and prevents kidney allograft rejection. however, it is ineffective in a sizable minority of patients. although t-cell receptor and cd28 engagement are known to initiate t-cell activation, many human antigen-experienced t-cells lose cd28, and can be activated independent of cd28 signals. we posit that these cells are central drivers of costimulation blockade resistant rejection (cobrr) and propose that cobrr might relate to a ... | 2015 | 25801976 |
| evaluation of a viral microarray based on simultaneous extraction and amplification of viral nucleotide acid for detecting human herpesviruses and enteroviruses. | in this study, a viral microarray based assay was developed to detect the human herpesviruses and enteroviruses associated with central nervous system infections, including herpes simplex virus type 1, type 2 (hsv1 and hsv2), epstein-barr virus (ebv), cytomegalovirus (cmv), enterovirus 71 (ev71), coxsackievirus a 16 (ca16) and b 5(cb5). the dna polymerase gene of human herpesviruses and 5'-untranslated region of enteroviruses were selected as the targets to design primers and probes. human herpe ... | 2015 | 25774509 |
| [disseminated histoplamosis in adolescent mimicking granulomatosis with polyangiitis]. | systemic histoplasmosis is an invasive fungal infection that may mimic primary vasculitis, particularly granulomatosis with polyangiitis (gpa), and was rarely described in adult patients. we reported an immunocompetent patient with disseminated histoplasmosis mimicking gpa who fulfilled european league against rheumatism (eular)/pediatric rheumatology international trials organisation (printo)/pediatric rheumatology european society (pres) validated classification criteria. | 2015 | 25802128 |
| viral markers in patients with hemophagocytosis: a prospective study in a tertiary care hospital. | hemophagocytic syndrome (hps) is a rare clinicopathological condition characterized by the activation of macrophages with prominent hemophagocytosis in bone marrow and other reticulo-endothelial systems. hps can be familial or secondary to infections including viruses. | 2015 | 22771647 |
| copy number variation in cep57l1 predisposes to congenital absence of bilateral acl and pcl ligaments. | absence of the anterior (acl) or posterior cruciate ligament (pcl) are rare congenital malformations that result in knee joint instability, with a prevalence of 1.7 per 100,000 live births and can be associated with other lower-limb abnormalities such as acl agnesia and absence of the menisci of the knee. while a few cases of absence of acl/pcl are reported in the literature, a number of large familial case series of related conditions such as acl agnesia suggest a potential underlying monogenic ... | 2015 | 26561035 |
| the role of magt1 in genetic syndromes. | disturbances in magnesium homeostasis, often linked to altered expression and/or function of magnesium channels, have been implicated in a plethora of diseases. this review focuses on magnesium transporter 1 (magt1), as recently described changes in this gene have further extended our understanding of the role of magnesium in human health and disease. the identification of genetic changes and their functional consequences in patients with immunodeficiency revealed that magnesium and magt1 are ke ... | 2015 | 26422833 |
| epstein-barr virus reactivation in allogeneic stem cell transplantation is highly related to cytomegalovirus reactivation. | monitoring of epstein-barr virus (ebv) load and pre-emptive rituximab is an appropriate approach to prevent post-transplant lymphoproliferative disease (ptld) occurring after hematopoietic stem cell transplantation (hsct). this pre-emptive approach, based on ebv-dna monitoring through a quantitative polymerase chain reaction, was applied to 101 consecutive patients who underwent allo hsct at our institute (median age 50). a single infusion of rituximab was administered to 11 of 16 patients who w ... | 2015 | 23781897 |
| real-time pcr of cytomegalovirus and epstein-barr virus in adult egyptian patients with systemic lupus erythematosus. | infections may act as environmental triggers for induction of systemic lupus erythematosus (sle). we sought to explore the relative frequencies of epstein-barr virus (ebv) and human cytomegalovirus (cmv) in adult egyptian patients with sle and their correlation with disease activity and damage. | 2015 | 24341363 |
| human cytomegalovirus and epstein-barr virus infection impact on (18)f-fdg pet/ct suvmax, ct volumetric and kras-based parameters of patients with locally advanced rectal cancer treated with neoadjuvant therapy. | it has long been debated whether human cytomegalovirus (hcmv) and epstein-barr virus (ebv) are associated with rectal cancer. the gene products of hcmv and ebv contribute to cell-cycle progression, mutagenesis, angiogenesis and immune evasion. the aim of this prospective study was to analyse the association between infection of a tumour by hcmv and ebv and clinical, histological, metabolic ((18)f-fdg uptake), volumetric (from ct) and molecular (kras status) features and long-term outcomes in a h ... | 2015 | 25269837 |
| graft versus leukemia response without graft-versus-host disease elicited by adoptively transferred multivirus-specific t-cells. | a 12-year-old boy with refractory acute lymphoblastic leukemia received a haploidentical transplant from his mother. as prophylaxis for epstein-barr virus (ebv), cytomegalovirus (cmv) and adenovirus, he received ex vivo expanded virus-specific donor t cells 3.5 months after transplant. four weeks later leukemic blasts bearing the e2a deletion, identified by fluorescent in situ hybridization (fish), appeared transiently in the blood followed by a fish-negative hematological remission, which was s ... | 2015 | 25266309 |
| epstein-barr virus reactivation in critically ill immunocompetent patients. | herpes viruses can be reactivated among immunocompetent patients in intensive care unit (icu). cytomegalovirus (cmv) and herpes simplex virus (hsv) have been the most studied. we hypothesized that epstein-barr virus (ebv) could also be reactivated in immunocompetent patients during their stay in icu and that this would be associated with morbidity and mortality. | 2015 | 25179711 |
| a heterogeneous hierarchy of co-regulatory receptors regulates exhaustion of hcv-specific cd8 t cells in patients with chronic hepatitis c. | the functionality of virus-specific t cells is regulated by a sophisticated network of an expanding repertoire of co-regulatory receptors, which could be harnessed for immunotherapeutic applications. however, targeting particular pathways during persistent virus infections has resulted in variable outcomes. the extent to which t cell exhaustion can be reversed, by targeting multiple co-regulatory pathways, still remains not fully investigated. | 2015 | 25131771 |
| viral surveillance and subclinical viral infection in pediatric kidney transplantation. | the more potent immunosuppressive therapy that has successfully reduced the incidence of acute rejection and improved graft outcomes has also resulted in a higher incidence of viral complications. sensitive molecular methods now allow for the detection of subclinical viral infection, which is increasingly recognized due to the adoption of routine post-transplant viral surveillance protocols. the goal of viral surveillance is the detection of subclinical viral infection that triggers an intervent ... | 2015 | 25125226 |
| epstein-barr virus associated peri-implantitis: a split-mouth study. | herpesviral-bacterial synergism may play a potential role in periodontitis and peri-implantitis (pi) etiopathogenesis. pi lesions can worsen depending on specific microbial challenge and host susceptibility. this cross-sectional split-mouth study aimed to substantiate herpesviral-bacterial co-infection in pi patients and assess associations with periodontopathogen salivary contamination. | 2015 | 24802631 |
| an observational study of inflammation in the central nervous system in patients with bipolar disorder. | the potential influence of infections and immunological changes on the aetiology and pathogenesis of bipolar disorder (bd) has been discussed. our aim was to detect intrathecal specific antibody synthesis against the neurotropic infectious agents that have previously been linked to bd. | 2015 | 25109751 |
| prevalence and risk factors of gammaherpesvirus infection in domestic cats in central europe. | gammaherpesviruses (ghvs) are a large group of dsdna viruses that can infect humans and several animal species. the two human ghvs, epstein-barr virus and kaposi's sarcoma-associated herpesvirus are known for their oncogenic properties in individuals with immunodeficiency. recently, the first feline ghv, felis catus gammaherpesvirus 1 (fcaghv1) was discovered and frequently found in domestic cats in australia, singapore and the usa. fcaghv1 is more likely to be detected in cats co-infected with ... | 2015 | 26377918 |
| inclusion bodies of recombinant epstein-barr virus capsid antigen p18 as potential immobilized antigens in enzyme immunoassays for detection of nasopharyngeal carcinoma. | development of indirect enzyme-linked immunosorbent assays (elisas) often utilizes synthetic peptides or recombinant proteins from escherichia coli as immobilized antigens. because inclusion bodies (ibs) formed during recombinant protein expression in e. coli are commonly thought as misfolded aggregates, only refolded proteins from ibs are used to develop new or in-house diagnostic assays. however, the promising utilities of ibs as nanomaterials and immobilized enzymes as shown in recent studies ... | 2015 | 26164385 |
| the b subunit of escherichia coli enterotoxin helps control the in vivo growth of solid tumors expressing the epstein-barr virus latent membrane protein 2a. | latent membrane protein 2a (lmp2a) is expressed on almost all epstein-barr virus (ebv)-associated tumors and is a potential target for immunotherapeutic intervention and vaccination. however, lmp2a is not efficiently processed and presented on major histocompatibility antigens class i molecules to generate potent cytotoxic t-lymphocytes (ctl) responses capable of killing these tumors. the b subunit of escherichia coli enterotoxin (etxb), causes rapid internalization and processing of membrane-bo ... | 2015 | 25641882 |
| the v-val subtype epstein-barr virus nuclear antigen 1 promotes cell survival after serum withdrawal. | epstein-barr virus (ebv) can establish latent infection and has been associated with various human cancers. epstein-barr nuclear antigen 1 (ebna1) is the only viral protein that is expressed in all ebv-associated malignant tissues. the n- and c-terminal domains of ebna1, which are connected by internal glycine/alanine-rich short repeat sequences of various sizes, show sequence divergence across ebv strains isolated from around the world. at least five subtypes have been described, according to t ... | 2015 | 25434292 |
| epstein-barr virus infection is equally distributed across the invasive ductal and invasive lobular forms of breast cancer. | the role of epstein-barr virus (ebv) in the pathogenesis of breast cancer is still unclear, although a growing body of evidence supports a link. the aim of this study was to investigate if ebv infection was more prevalent in invasive ductal carcinoma or invasive lobular carcinoma. an immunohistochemical marker for ebv (epstein-barr virus nuclear antigen 1 (ebna1) clone e1-2.5) was applied to a tissue micro array section. the tissue micro array contained 80 cases of invasive ductal carcinoma, and ... | 2015 | 26481274 |
| transcriptional activation of epstein-barr virus brlf1 by usf1 and rta. | during its lytic cycle, epstein-barr virus (ebv) expresses rta, a factor encoded by brlf1 that activates the transcription of viral lytic genes. we found that upstream stimulating factor (usf) binds to e1, one of the five e boxes located at - 79 in the brlf1 promoter (rp), to activate brlf1 transcription. furthermore, rta was shown to interact with usf1 in coimmunoprecipitation and glutathione s-transferase (gst)-pulldown assays, and confocal laser-scanning microscopy further confirmed that thes ... | 2015 | 26297580 |
| identification of the critical attribute(s) of ebv gp350 antigen required for elicitation of a neutralizing antibody response in vivo. | vaccine prophylaxis with ebv glycoprotein 350 (gp350) subunit plus adjuvant has been demonstrated clinically to protect individuals against infectious mononucleosis (im), but the specifications of the antigen required to elicit this protection has remained largely theoretical. previous studies have shown that antibodies to gp350 comprise the principle component of ebv-neutralizing sera. further, a murine monoclonal antibody against gp350 (clone 72a1) is able to prevent infection by the virus bot ... | 2015 | 26485517 |
| invasive fusobacterium necrophorum infections and lemièrre's syndrome: the role of thrombophilia and ebv. | the purpose of this investigation was to describe the clinical spectrum of invasive fusobacterium necrophorum infections and lemièrre's syndrome, to examine the role of underlying thrombophilia and concomitant mononucleosis in lemièrre's syndrome, and to describe thromboembolic complications. patients with invasive f. necrophorum infections were identified either prospectively or retrospectively through the regional database of clinical microbiology from 2000 to 2015. patient records were review ... | 2015 | 26272176 |
| epstein-barr virus association with peptic ulcer disease. | background. helicobacter pylori (hp) infection and nonsteroidal anti-inflammatory drugs (nsaid) use are considered the main risk to develop peptic ulcer disease (pud). however, pud also occurs in the absence of hp infection and/or nsaid use. recently, we have found evidence that epstein-barr virus (ebv) reactivation increases the risk to develop premalignant and malignant gastric lesions. objective. to study a possible association between ebv and pud. methods. antibodies against an ebv reactivat ... | 2015 | 26199856 |
| frequency of seropositivity against infectious agents amongst pemphigus vulgaris patients: a case-control study on strongyloides stercoralis, helicobacter pylori, toxoplasma gondii, leishmania major, and epstein-barr virus. | environmental and genetic factors may contribute to the pathogenesis of pemphigus vulgaris (pv) as an autoimmune disease. we aimed to determine rates of seropositivity for immunoglobulin g (igg) antibodies against a number of infectious agents in untreated and treated pv patients and in healthy individuals. | 2015 | 26175264 |
| case–control study of epstein–barr virus and helicobacter pylori serology in latin american patients with gastric disease. | chronic tissue damage induced by helicobacter pylori (hp)-driven inflammation is considered the main risk of gastric carcinoma (gc). epstein–barr virus (ebv) infection has also been associated with gc. in this study, we aim to address the role of ebv in inflammatory gc precursor lesions and its added risk to hp infection. | 2015 | 25996206 |